AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017749.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017749.01.T01 MTR_3g111540 89.548 354 14 1 1 331 1 354 0.0 632
MsG0380017749.01.T01 MTR_3g077500 30.063 316 200 7 24 324 61 370 2.70e-44 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017749.01.T01 AT1G48460 60.584 274 104 1 58 331 71 340 7.58e-120 349
MsG0380017749.01.T01 AT1G48460 53.650 274 86 2 58 331 71 303 1.70e-98 293
MsG0380017749.01.T01 AT5G63040 29.582 311 208 4 15 321 55 358 2.16e-34 129
MsG0380017749.01.T01 AT5G63040 29.582 311 208 4 15 321 55 358 2.63e-34 129

Find 79 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGAGGCTATTCCCTTCT+AGG 0.161760 3:+100296006 MsG0380017749.01.T01:CDS
AGTTCTAGTGTTCCTGTTAA+TGG 0.210562 3:+100293725 MsG0380017749.01.T01:CDS
GAGCTCTTACTTATGGGTTT+TGG 0.226459 3:+100293865 MsG0380017749.01.T01:CDS
AGACAAACCTCAAATATAAT+AGG 0.229602 3:-100295672 None:intergenic
AGCAGCACAGCCAACTAAAA+AGG 0.270995 3:-100295592 None:intergenic
TTAGAGCTGTACTGTAAATA+TGG 0.272745 3:-100295429 None:intergenic
CATCAACTTCTCGGCAAAAT+TGG 0.283065 3:-100295862 None:intergenic
AAGAGGAGAGCTCTTACTTA+TGG 0.285229 3:+100293858 MsG0380017749.01.T01:CDS
GTTCTAGTGTTCCTGTTAAT+GGG 0.303684 3:+100293726 MsG0380017749.01.T01:CDS
GAACCATTTCATGGTAAATC+AGG 0.312758 3:+100293764 MsG0380017749.01.T01:CDS
GAACGAAGTGGAGCTTTGTT+TGG 0.345152 3:+100295924 MsG0380017749.01.T01:CDS
TGCATACAATACCCAGTAAC+TGG 0.357271 3:-100295962 None:intergenic
TTTATTACTGACACGATGTT+TGG 0.357578 3:-100293350 None:intergenic
GGTGTGCGAATCAATTATTT+TGG 0.360901 3:-100296239 None:intergenic
CCACTTCGTTCTAGTACTTC+AGG 0.361982 3:-100295912 None:intergenic
TCTAGTCCCTATTATATTTG+AGG 0.362027 3:+100295665 MsG0380017749.01.T01:CDS
GCTTCTGATTCCAATGTAAA+TGG 0.366214 3:+100293677 MsG0380017749.01.T01:CDS
ATTTGCATTTGAGAGACATT+TGG 0.369154 3:+100295617 MsG0380017749.01.T01:CDS
TACTGGGTATTGTATGCATT+TGG 0.376974 3:+100295967 MsG0380017749.01.T01:CDS
AACGGGTTGGAACCATTTCA+TGG 0.395145 3:+100293755 MsG0380017749.01.T01:CDS
TGGAGCTTTGTTTGGAATGA+TGG 0.401307 3:+100295932 MsG0380017749.01.T01:CDS
GAACCTGATTTACCATGAAA+TGG 0.410530 3:-100293767 None:intergenic
ACCTCTGCTTTCCTCACAAC+GGG 0.412478 3:+100295489 MsG0380017749.01.T01:CDS
AGAGGAGAGCTCTTACTTAT+GGG 0.413551 3:+100293859 MsG0380017749.01.T01:CDS
CGGAGCGGATAACATTTATA+AGG 0.421430 3:+100296086 MsG0380017749.01.T01:three_prime_UTR
AAAGATGATATGAATGCAAC+AGG 0.422615 3:-100293890 None:intergenic
GACAAACCTCAAATATAATA+GGG 0.437022 3:-100295671 None:intergenic
GAGAAGTGAAGTGGTTGAGA+GGG 0.440340 3:-100293328 None:intergenic
TACAGTACAGCTCTAAAAGA+TGG 0.450227 3:+100295436 MsG0380017749.01.T01:CDS
TTTGATTTCAGCATTCAAAC+AGG 0.454931 3:+100293598 MsG0380017749.01.T01:intron
CATAGAGTACATCAACTTCT+CGG 0.455751 3:-100295871 None:intergenic
AAAAGATGGGGTCTCATCAC+TGG 0.458268 3:+100295450 MsG0380017749.01.T01:CDS
ACTTCCCTTCTTCTACAGAT+TGG 0.459140 3:-100293814 None:intergenic
AAGGCGATTAACTTCAATGT+AGG 0.460653 3:+100296105 MsG0380017749.01.T01:three_prime_UTR
GAAGTTGATGTACTCTATGA+AGG 0.469649 3:+100295875 MsG0380017749.01.T01:CDS
CAGAGGTTATATATCCTTTG+AGG 0.492149 3:-100295473 None:intergenic
TTCCAATGCACCATTTACAT+TGG 0.492222 3:-100293687 None:intergenic
GGAGAAGTGAAGTGGTTGAG+AGG 0.505328 3:-100293329 None:intergenic
CATTGATGACGTTTCTCTTG+AGG 0.505870 3:+100295991 MsG0380017749.01.T01:CDS
ACAGTACAGCTCTAAAAGAT+GGG 0.516922 3:+100295437 MsG0380017749.01.T01:CDS
GTCGGTGGTTCGCATGGCAG+CGG 0.518805 3:+100295554 MsG0380017749.01.T01:CDS
ACAGACGTGGGTCATCGTGC+TGG 0.519318 3:+100295640 MsG0380017749.01.T01:CDS
ATTCTGCCACAGTTCTTCGT+TGG 0.519550 3:+100293914 MsG0380017749.01.T01:CDS
ATCTGTAGAAGAAGGGAAGT+TGG 0.519792 3:+100293817 MsG0380017749.01.T01:CDS
CTTCGTTGGCACTGTTGTTG+CGG 0.536995 3:+100293928 MsG0380017749.01.T01:CDS
TTCTAGTGTTCCTGTTAATG+GGG 0.540217 3:+100293727 MsG0380017749.01.T01:CDS
ACACACCAATCTGTAGAAGA+AGG 0.540836 3:+100293809 MsG0380017749.01.T01:CDS
GGTCTCATCACTGGCCTCAA+AGG 0.549169 3:+100295459 MsG0380017749.01.T01:CDS
TTCCAATGTAAATGGTGCAT+TGG 0.552698 3:+100293685 MsG0380017749.01.T01:CDS
GAGCTCTCCTCTTGTTTGAA+CGG 0.557951 3:-100293848 None:intergenic
GTCACATACAAAAGAAGAAG+AGG 0.559688 3:-100295528 None:intergenic
GACCTGGTCGGTGGTTCGCA+TGG 0.562381 3:+100295548 MsG0380017749.01.T01:CDS
TGCCATGCGAACCACCGACC+AGG 0.563381 3:-100295550 None:intergenic
TGTACTGTAAATATGGCCTC+TGG 0.563744 3:-100295422 None:intergenic
GGAAGAGTGTTGTACATAGT+CGG 0.575600 3:+100296066 MsG0380017749.01.T01:three_prime_UTR
AAGTTGATGTACTCTATGAA+GGG 0.577690 3:+100295876 MsG0380017749.01.T01:CDS
TCCGAGGCACTGTTCTATGT+CGG 0.580851 3:+100295366 MsG0380017749.01.T01:CDS
ATCTGCTCCGTTCAAACAAG+AGG 0.588809 3:+100293841 MsG0380017749.01.T01:CDS
GAGAGACATTTGGACAGACG+TGG 0.588933 3:+100295627 MsG0380017749.01.T01:CDS
CACACCAATCTGTAGAAGAA+GGG 0.595884 3:+100293810 MsG0380017749.01.T01:CDS
TTTCTGCTACTGGCCTAGAA+GGG 0.597512 3:-100296019 None:intergenic
TCAAATATAATAGGGACTAG+AGG 0.608339 3:-100295663 None:intergenic
TGTGACTTCATTCTCTTCCG+AGG 0.611821 3:+100295350 MsG0380017749.01.T01:CDS
AACCTCTGCTTTCCTCACAA+CGG 0.613720 3:+100295488 MsG0380017749.01.T01:CDS
TCTAGTGTTCCTGTTAATGG+GGG 0.617974 3:+100293728 MsG0380017749.01.T01:CDS
AGTTGATGTACTCTATGAAG+GGG 0.619565 3:+100295877 MsG0380017749.01.T01:CDS
TCCGACATAGAACAGTGCCT+CGG 0.628444 3:-100295367 None:intergenic
AGGTGGCTGATCGTGTCCAG+AGG 0.630587 3:+100295406 MsG0380017749.01.T01:CDS
TCTAGTACTTCAGGTGACGC+AGG 0.632274 3:-100295903 None:intergenic
TTTGTATGTGACCTGGTCGG+TGG 0.632802 3:+100295539 MsG0380017749.01.T01:CDS
CGATGTTTGGAGAAGTGAAG+TGG 0.648063 3:-100293337 None:intergenic
CCTGAAGTACTAGAACGAAG+TGG 0.656210 3:+100295912 MsG0380017749.01.T01:CDS
CAGTACAGCTCTAAAAGATG+GGG 0.663248 3:+100295438 MsG0380017749.01.T01:CDS
AGAGACATTTGGACAGACGT+GGG 0.672518 3:+100295628 MsG0380017749.01.T01:CDS
ATACAAAAGAAGAAGAGGAA+CGG 0.679901 3:-100295523 None:intergenic
ACCCGTTGTGAGGAAAGCAG+AGG 0.694025 3:-100295490 None:intergenic
ACAGTGCCAACGAAGAACTG+TGG 0.710210 3:-100293920 None:intergenic
AGTGTTGTACATAGTCGGAG+CGG 0.720519 3:+100296071 MsG0380017749.01.T01:three_prime_UTR
AATAATTGATTCGCACACCG+TGG 0.724531 3:+100296243 MsG0380017749.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATGATTTGATTTTAAA+AGG + Chr3:100293956-100293975 MsG0380017749.01.T01:CDS 10.0%
!!! ATTTATAACAATTTGTTGAA+TGG - Chr3:100293572-100293591 None:intergenic 15.0%
!!! GAAATTATTTTGTTTACTTA+AGG - Chr3:100294854-100294873 None:intergenic 15.0%
!! GTTAATACCAAATTAGTTAT+TGG + Chr3:100294752-100294771 MsG0380017749.01.T01:intron 20.0%
!! TATAAAGCCAATAACTAATT+TGG - Chr3:100294762-100294781 None:intergenic 20.0%
!! TGGGATTGAAAAAATTTAAA+AGG + Chr3:100293642-100293661 MsG0380017749.01.T01:CDS 20.0%
!!! AAAGACATTGTTTATTTTCT+CGG - Chr3:100296197-100296216 None:intergenic 20.0%
!!! AAATGTACGATTTTTGTTTT+TGG + Chr3:100293527-100293546 MsG0380017749.01.T01:intron 20.0%
!!! AATGTACGATTTTTGTTTTT+GGG + Chr3:100293528-100293547 MsG0380017749.01.T01:intron 20.0%
!!! ATGATAATTGTTTTTGTTGT+AGG + Chr3:100294606-100294625 MsG0380017749.01.T01:intron 20.0%
!!! ATTAAAGAACCTTCATTTTT+GGG + Chr3:100293623-100293642 MsG0380017749.01.T01:CDS 20.0%
!!! CAGAATTTTATTTTACTTGT+CGG - Chr3:100294568-100294587 None:intergenic 20.0%
! AAAAGAATGGAGTTACATAA+AGG + Chr3:100295239-100295258 MsG0380017749.01.T01:intron 25.0%
! AAGAAGAAAAAAAGTTAGTG+TGG - Chr3:100293429-100293448 None:intergenic 25.0%
! AGACAAACCTCAAATATAAT+AGG - Chr3:100295675-100295694 None:intergenic 25.0%
! ATCAGCAATATAAACACATT+AGG - Chr3:100294675-100294694 None:intergenic 25.0%
! CCATTCATAATTACAAAACA+TGG - Chr3:100294384-100294403 None:intergenic 25.0%
! CCATTCATAATTTCAAAACA+TGG - Chr3:100294968-100294987 None:intergenic 25.0%
! GACAAACCTCAAATATAATA+GGG - Chr3:100295674-100295693 None:intergenic 25.0%
! GTTAATGCCAAATTATTTAG+TGG + Chr3:100294192-100294211 MsG0380017749.01.T01:intron 25.0%
! TATATGGTAGTATGTTGTTA+AGG + Chr3:100294716-100294735 MsG0380017749.01.T01:intron 25.0%
!! AGATATCAGAAACAGAATTT+TGG + Chr3:100293488-100293507 MsG0380017749.01.T01:intron 25.0%
!! CCATGTTTTGTAATTATGAA+TGG + Chr3:100294381-100294400 MsG0380017749.01.T01:intron 25.0%
!! GATATCAGAAACAGAATTTT+GGG + Chr3:100293489-100293508 MsG0380017749.01.T01:intron 25.0%
!! GATTAAAGAACCTTCATTTT+TGG + Chr3:100293622-100293641 MsG0380017749.01.T01:CDS 25.0%
!! TAAATGATAGTTTGTGACAT+AGG + Chr3:100294043-100294062 MsG0380017749.01.T01:intron 25.0%
!! TATTTTACTTGTCGGAAATT+CGG - Chr3:100294560-100294579 None:intergenic 25.0%
!! TCACAATATCTGCATTTTAT+TGG - Chr3:100295335-100295354 None:intergenic 25.0%
!! TTTATGTAACTCCATTCTTT+TGG - Chr3:100295240-100295259 None:intergenic 25.0%
!!! ATATTCTGGTTGTTATTTCA+CGG - Chr3:100294640-100294659 None:intergenic 25.0%
!!! CCATGTTTTGAAATTATGAA+TGG + Chr3:100294965-100294984 MsG0380017749.01.T01:intron 25.0%
!!! GAAAATTACTGAGTTTTGTA+TGG + Chr3:100296038-100296057 MsG0380017749.01.T01:exon 25.0%
!!! TTTTTCAATCCCAAAAATGA+AGG - Chr3:100293635-100293654 None:intergenic 25.0%
AAAGATGATATGAATGCAAC+AGG - Chr3:100293893-100293912 None:intergenic 30.0%
AAGAGTTATACAACAAACAG+AGG - Chr3:100294480-100294499 None:intergenic 30.0%
AGAAATGCAGAGAAATTCAA+GGG - Chr3:100295009-100295028 None:intergenic 30.0%
AGTTCTTTGTCTACACTATA+TGG + Chr3:100294700-100294719 MsG0380017749.01.T01:intron 30.0%
ATACAAAAGAAGAAGAGGAA+CGG - Chr3:100295526-100295545 None:intergenic 30.0%
ATTTGCATTTGAGAGACATT+TGG + Chr3:100295617-100295636 MsG0380017749.01.T01:CDS 30.0%
CATCAAGCCACTAAATAATT+TGG - Chr3:100294202-100294221 None:intergenic 30.0%
GAAATTCTGTTCCAAAAGAA+TGG + Chr3:100295226-100295245 MsG0380017749.01.T01:intron 30.0%
TAGAAATGCAGAGAAATTCA+AGG - Chr3:100295010-100295029 None:intergenic 30.0%
TCAAATATAATAGGGACTAG+AGG - Chr3:100295666-100295685 None:intergenic 30.0%
TCAATTACCAATAGTCTTTG+AGG - Chr3:100294807-100294826 None:intergenic 30.0%
TCTAGTCCCTATTATATTTG+AGG + Chr3:100295665-100295684 MsG0380017749.01.T01:CDS 30.0%
TGAAACGATCAATTATCAAC+AGG - Chr3:100294255-100294274 None:intergenic 30.0%
TGAGTTCTCACTAACATATA+TGG + Chr3:100295088-100295107 MsG0380017749.01.T01:intron 30.0%
TTAGAGCTGTACTGTAAATA+TGG - Chr3:100295432-100295451 None:intergenic 30.0%
TTTATTACTGACACGATGTT+TGG - Chr3:100293353-100293372 None:intergenic 30.0%
! AATTTAAAGCACAGAACAGA+AGG - Chr3:100294017-100294036 None:intergenic 30.0%
! ATTTTGCCAAAATCGCTAAA+AGG - Chr3:100295143-100295162 None:intergenic 30.0%
! CAAAGACTATTGGTAATTGA+TGG + Chr3:100294807-100294826 MsG0380017749.01.T01:intron 30.0%
! GTAACTGATAATGTTTATGC+TGG - Chr3:100294297-100294316 None:intergenic 30.0%
! TGTATACCTTTTAGCGATTT+TGG + Chr3:100295134-100295153 MsG0380017749.01.T01:intron 30.0%
! TTCTGTTCTGTGCTTTAAAT+TGG + Chr3:100294016-100294035 MsG0380017749.01.T01:intron 30.0%
! TTTGATTTCAGCATTCAAAC+AGG + Chr3:100293598-100293617 MsG0380017749.01.T01:intron 30.0%
! TTTGTTTGTGCTGTTAAATG+TGG + Chr3:100295269-100295288 MsG0380017749.01.T01:intron 30.0%
!! AAGTTGATGTACTCTATGAA+GGG + Chr3:100295876-100295895 MsG0380017749.01.T01:CDS 30.0%
!! ACTACTTGTTGTTATCTTTG+TGG + Chr3:100294934-100294953 MsG0380017749.01.T01:intron 30.0%
!! ACTGAGTTTTGTATGGAATT+GGG + Chr3:100296045-100296064 MsG0380017749.01.T01:exon 30.0%
!! CTGTTTGTTGTATAACTCTT+GGG + Chr3:100294480-100294499 MsG0380017749.01.T01:intron 30.0%
!! TACTGAGTTTTGTATGGAAT+TGG + Chr3:100296044-100296063 MsG0380017749.01.T01:exon 30.0%
!! TCTGTTTGTTGTATAACTCT+TGG + Chr3:100294479-100294498 MsG0380017749.01.T01:intron 30.0%
!!! TATTTTGGCTGTAAACAAAC+AGG - Chr3:100296227-100296246 None:intergenic 30.0%
AAGCTAACAACAACTAGTGT+TGG - Chr3:100294348-100294367 None:intergenic 35.0%
AAGGCGATTAACTTCAATGT+AGG + Chr3:100296105-100296124 MsG0380017749.01.T01:three_prime_UTR 35.0%
ACAAATCCGATAGAATAACG+TGG - Chr3:100295175-100295194 None:intergenic 35.0%
ATAACAACAAGTAGTGTCAG+TGG - Chr3:100294929-100294948 None:intergenic 35.0%
ATGGAGTTACATAAAGGTCA+TGG + Chr3:100295245-100295264 MsG0380017749.01.T01:intron 35.0%
CAGAGGTTATATATCCTTTG+AGG - Chr3:100295476-100295495 None:intergenic 35.0%
CATAGAGTACATCAACTTCT+CGG - Chr3:100295874-100295893 None:intergenic 35.0%
GAAATGCAGAGAAATTCAAG+GGG - Chr3:100295008-100295027 None:intergenic 35.0%
GAACCATTTCATGGTAAATC+AGG + Chr3:100293764-100293783 MsG0380017749.01.T01:CDS 35.0%
GAACCTGATTTACCATGAAA+TGG - Chr3:100293770-100293789 None:intergenic 35.0%
GCTTCTGATTCCAATGTAAA+TGG + Chr3:100293677-100293696 MsG0380017749.01.T01:CDS 35.0%
GGAACAGCAATCATATATTC+TGG - Chr3:100294654-100294673 None:intergenic 35.0%
GTCACATACAAAAGAAGAAG+AGG - Chr3:100295531-100295550 None:intergenic 35.0%
GTGTTTCCACGTTATTCTAT+CGG + Chr3:100295166-100295185 MsG0380017749.01.T01:intron 35.0%
GTTCTAGTGTTCCTGTTAAT+GGG + Chr3:100293726-100293745 MsG0380017749.01.T01:CDS 35.0%
GTTCTCACTAACATATATGG+AGG + Chr3:100295091-100295110 MsG0380017749.01.T01:intron 35.0%
TCAATTATCAACAGGCTTTG+AGG - Chr3:100294247-100294266 None:intergenic 35.0%
TCTCACTCAGATAACATACA+TGG + Chr3:100294438-100294457 MsG0380017749.01.T01:intron 35.0%
TGAGAGGAATAACAACAACT+TGG + Chr3:100295753-100295772 MsG0380017749.01.T01:intron 35.0%
TGTATAACTCTTGGGTATCT+TGG + Chr3:100294488-100294507 MsG0380017749.01.T01:intron 35.0%
TTCCAATGCACCATTTACAT+TGG - Chr3:100293690-100293709 None:intergenic 35.0%
TTCCAATGTAAATGGTGCAT+TGG + Chr3:100293685-100293704 MsG0380017749.01.T01:CDS 35.0%
TTCTAGTGTTCCTGTTAATG+GGG + Chr3:100293727-100293746 MsG0380017749.01.T01:CDS 35.0%
! ACTCAGTAATTTTCTGCTAC+TGG - Chr3:100296032-100296051 None:intergenic 35.0%
! ACTGATAATGTTTATGCTGG+AGG - Chr3:100294294-100294313 None:intergenic 35.0%
! AGTTCTAGTGTTCCTGTTAA+TGG + Chr3:100293725-100293744 MsG0380017749.01.T01:CDS 35.0%
! AGTTGATGTACTCTATGAAG+GGG + Chr3:100295877-100295896 MsG0380017749.01.T01:CDS 35.0%
! ATGGTGATTTTCCAGTTACT+GGG + Chr3:100295951-100295970 MsG0380017749.01.T01:CDS 35.0%
! CAGGTTCTGTCTCTTTTTAT+GGG + Chr3:100293783-100293802 MsG0380017749.01.T01:CDS 35.0%
! CTTTTTGTCTACACTTTGGT+AGG + Chr3:100294139-100294158 MsG0380017749.01.T01:intron 35.0%
! GGTGTGCGAATCAATTATTT+TGG - Chr3:100296242-100296261 None:intergenic 35.0%
! GTTCTGTGCTTTAAATTGGA+TGG + Chr3:100294020-100294039 MsG0380017749.01.T01:intron 35.0%
! TACTGGGTATTGTATGCATT+TGG + Chr3:100295967-100295986 MsG0380017749.01.T01:CDS 35.0%
! TCAGGTTCTGTCTCTTTTTA+TGG + Chr3:100293782-100293801 MsG0380017749.01.T01:CDS 35.0%
! TGCTCTTTTTGTCTACACTT+TGG + Chr3:100294135-100294154 MsG0380017749.01.T01:intron 35.0%
! TTCTTCTTTTGTATGTGACC+TGG + Chr3:100295532-100295551 MsG0380017749.01.T01:CDS 35.0%
!! ACAGTACAGCTCTAAAAGAT+GGG + Chr3:100295437-100295456 MsG0380017749.01.T01:CDS 35.0%
!! ACTAGTTGTTGTTAGCTTTG+TGG + Chr3:100294350-100294369 MsG0380017749.01.T01:intron 35.0%
!! GAAGTTGATGTACTCTATGA+AGG + Chr3:100295875-100295894 MsG0380017749.01.T01:CDS 35.0%
!! TACAGTACAGCTCTAAAAGA+TGG + Chr3:100295436-100295455 MsG0380017749.01.T01:CDS 35.0%
!! TGAAATTGAATTGACTTGCC+AGG + Chr3:100295809-100295828 MsG0380017749.01.T01:intron 35.0%
AAGAGGAGAGCTCTTACTTA+TGG + Chr3:100293858-100293877 MsG0380017749.01.T01:CDS 40.0%
ACACACCAATCTGTAGAAGA+AGG + Chr3:100293809-100293828 MsG0380017749.01.T01:CDS 40.0%
ACTTCCCTTCTTCTACAGAT+TGG - Chr3:100293817-100293836 None:intergenic 40.0%
AGAGGAGAGCTCTTACTTAT+GGG + Chr3:100293859-100293878 MsG0380017749.01.T01:CDS 40.0%
AGTGTAGACAAAAAGAGCAG+CGG - Chr3:100294134-100294153 None:intergenic 40.0%
ATCTGTAGAAGAAGGGAAGT+TGG + Chr3:100293817-100293836 MsG0380017749.01.T01:CDS 40.0%
ATGCGAACCTCAAAGACTAT+TGG + Chr3:100294797-100294816 MsG0380017749.01.T01:intron 40.0%
CACACCAATCTGTAGAAGAA+GGG + Chr3:100293810-100293829 MsG0380017749.01.T01:CDS 40.0%
CATCAACTTCTCGGCAAAAT+TGG - Chr3:100295865-100295884 None:intergenic 40.0%
CCTGTTAATGGGGGTTATAA+CGG + Chr3:100293737-100293756 MsG0380017749.01.T01:CDS 40.0%
CGGAGCGGATAACATTTATA+AGG + Chr3:100296086-100296105 MsG0380017749.01.T01:three_prime_UTR 40.0%
CTGTTAATGGGGGTTATAAC+GGG + Chr3:100293738-100293757 MsG0380017749.01.T01:CDS 40.0%
GCTGATACAATCTGTATACC+TGG - Chr3:100295830-100295849 None:intergenic 40.0%
TCTAGTGTTCCTGTTAATGG+GGG + Chr3:100293728-100293747 MsG0380017749.01.T01:CDS 40.0%
TGCATACAATACCCAGTAAC+TGG - Chr3:100295965-100295984 None:intergenic 40.0%
TGTACTGTAAATATGGCCTC+TGG - Chr3:100295425-100295444 None:intergenic 40.0%
TGTTTCCATGGTGTTCTGTT+GGG + Chr3:100294519-100294538 MsG0380017749.01.T01:intron 40.0%
TTGGCAAAACTGTGTTTCCA+TGG + Chr3:100294507-100294526 MsG0380017749.01.T01:intron 40.0%
! ACTTGCAACATTTTTGCAGG+TGG + Chr3:100295389-100295408 MsG0380017749.01.T01:CDS 40.0%
! CAATTTTCAAACCCGTTGTG+AGG - Chr3:100295503-100295522 None:intergenic 40.0%
! CGCTAAAAGGTATACAGCAA+AGG - Chr3:100295130-100295149 None:intergenic 40.0%
! CTAACAACAACTAGTGTTGG+TGG - Chr3:100294345-100294364 None:intergenic 40.0%
! GAGCTCTTACTTATGGGTTT+TGG + Chr3:100293865-100293884 MsG0380017749.01.T01:CDS 40.0%
! GATGGTGATTTTCCAGTTAC+TGG + Chr3:100295950-100295969 MsG0380017749.01.T01:CDS 40.0%
! TCTTTTGTATGTGACCTGGT+CGG + Chr3:100295536-100295555 MsG0380017749.01.T01:CDS 40.0%
!! AATAATTGATTCGCACACCG+TGG + Chr3:100296243-100296262 MsG0380017749.01.T01:three_prime_UTR 40.0%
!! CAGTACAGCTCTAAAAGATG+GGG + Chr3:100295438-100295457 MsG0380017749.01.T01:CDS 40.0%
!! CATTGATGACGTTTCTCTTG+AGG + Chr3:100295991-100296010 MsG0380017749.01.T01:CDS 40.0%
!! CTGCATTTCTAGTGCTTCTT+GGG + Chr3:100295019-100295038 MsG0380017749.01.T01:intron 40.0%
!! GCTATAGCTCCCTTTTTAGT+TGG + Chr3:100295582-100295601 MsG0380017749.01.T01:CDS 40.0%
!! GGAAGAGTGTTGTACATAGT+CGG + Chr3:100296066-100296085 MsG0380017749.01.T01:three_prime_UTR 40.0%
!! GTTTTAGATGTAGACTCTCG+TGG + Chr3:100295042-100295061 MsG0380017749.01.T01:intron 40.0%
!! TCTGCATTTCTAGTGCTTCT+TGG + Chr3:100295018-100295037 MsG0380017749.01.T01:intron 40.0%
!! TGCATTTCTAGTGCTTCTTG+GGG + Chr3:100295020-100295039 MsG0380017749.01.T01:intron 40.0%
!! TGGAGCTTTGTTTGGAATGA+TGG + Chr3:100295932-100295951 MsG0380017749.01.T01:CDS 40.0%
AAAACCCCAACAGAACACCA+TGG - Chr3:100294527-100294546 None:intergenic 45.0%
AAAAGATGGGGTCTCATCAC+TGG + Chr3:100295450-100295469 MsG0380017749.01.T01:CDS 45.0%
AACCTCTGCTTTCCTCACAA+CGG + Chr3:100295488-100295507 MsG0380017749.01.T01:CDS 45.0%
ACTTTGGTAGGACACTGTTG+AGG + Chr3:100294151-100294170 MsG0380017749.01.T01:intron 45.0%
AGAGACATTTGGACAGACGT+GGG + Chr3:100295628-100295647 MsG0380017749.01.T01:CDS 45.0%
AGCAGCACAGCCAACTAAAA+AGG - Chr3:100295595-100295614 None:intergenic 45.0%
AGTGGTAAGTACAGAACCAG+AGG - Chr3:100294911-100294930 None:intergenic 45.0%
AGTGTTGTACATAGTCGGAG+CGG + Chr3:100296071-100296090 MsG0380017749.01.T01:three_prime_UTR 45.0%
ATCTGCTCCGTTCAAACAAG+AGG + Chr3:100293841-100293860 MsG0380017749.01.T01:CDS 45.0%
ATTCTGCCACAGTTCTTCGT+TGG + Chr3:100293914-100293933 MsG0380017749.01.T01:CDS 45.0%
CCACTTCGTTCTAGTACTTC+AGG - Chr3:100295915-100295934 None:intergenic 45.0%
CCGTTATAACCCCCATTAAC+AGG - Chr3:100293740-100293759 None:intergenic 45.0%
CGATGTTTGGAGAAGTGAAG+TGG - Chr3:100293340-100293359 None:intergenic 45.0%
GAGAAGTGAAGTGGTTGAGA+GGG - Chr3:100293331-100293350 None:intergenic 45.0%
GCAGCACAGCCAACTAAAAA+GGG - Chr3:100295594-100295613 None:intergenic 45.0%
GTGTTTCCATGGTGTTCTGT+TGG + Chr3:100294518-100294537 MsG0380017749.01.T01:intron 45.0%
GTTTCCATGGTGTTCTGTTG+GGG + Chr3:100294520-100294539 MsG0380017749.01.T01:intron 45.0%
TCTTGAGGCTATTCCCTTCT+AGG + Chr3:100296006-100296025 MsG0380017749.01.T01:CDS 45.0%
TGTGACTTCATTCTCTTCCG+AGG + Chr3:100295350-100295369 MsG0380017749.01.T01:CDS 45.0%
! CCTGAAGTACTAGAACGAAG+TGG + Chr3:100295912-100295931 MsG0380017749.01.T01:CDS 45.0%
! CGGACTTGCAACATTTTTGC+AGG + Chr3:100295386-100295405 MsG0380017749.01.T01:CDS 45.0%
! GAGCTCTCCTCTTGTTTGAA+CGG - Chr3:100293851-100293870 None:intergenic 45.0%
! TTTCTGCTACTGGCCTAGAA+GGG - Chr3:100296022-100296041 None:intergenic 45.0%
!! AACGGGTTGGAACCATTTCA+TGG + Chr3:100293755-100293774 MsG0380017749.01.T01:CDS 45.0%
!! GAACGAAGTGGAGCTTTGTT+TGG + Chr3:100295924-100295943 MsG0380017749.01.T01:CDS 45.0%
!! TAATGGGGGTTATAACGGGT+TGG + Chr3:100293742-100293761 MsG0380017749.01.T01:CDS 45.0%
!! TCAGTTCTGAAGCAAACCTC+TGG + Chr3:100294892-100294911 MsG0380017749.01.T01:intron 45.0%
!! TTTTCTGCTACTGGCCTAGA+AGG - Chr3:100296023-100296042 None:intergenic 45.0%
ACAGTGCCAACGAAGAACTG+TGG - Chr3:100293923-100293942 None:intergenic 50.0%
ACCTCTGCTTTCCTCACAAC+GGG + Chr3:100295489-100295508 MsG0380017749.01.T01:CDS 50.0%
ATGCAGCTTGACGAGTCTAC+TGG + Chr3:100295728-100295747 MsG0380017749.01.T01:intron 50.0%
CTTCGTTGGCACTGTTGTTG+CGG + Chr3:100293928-100293947 MsG0380017749.01.T01:CDS 50.0%
CTTTCGCAGTCACAAGCAAC+TGG + Chr3:100293449-100293468 MsG0380017749.01.T01:intron 50.0%
GAGAGACATTTGGACAGACG+TGG + Chr3:100295627-100295646 MsG0380017749.01.T01:CDS 50.0%
GGAGAAGTGAAGTGGTTGAG+AGG - Chr3:100293332-100293351 None:intergenic 50.0%
GGTGGTCAGTACAGAACTAG+AGG - Chr3:100294327-100294346 None:intergenic 50.0%
TCCGACATAGAACAGTGCCT+CGG - Chr3:100295370-100295389 None:intergenic 50.0%
TCTAGTACTTCAGGTGACGC+AGG - Chr3:100295906-100295925 None:intergenic 50.0%
TTTGTATGTGACCTGGTCGG+TGG + Chr3:100295539-100295558 MsG0380017749.01.T01:CDS 50.0%
!! TCCGAGGCACTGTTCTATGT+CGG + Chr3:100295366-100295385 MsG0380017749.01.T01:CDS 50.0%
ACCCGTTGTGAGGAAAGCAG+AGG - Chr3:100295493-100295512 None:intergenic 55.0%
! GACGAGTCTACTGGTCTGAG+AGG + Chr3:100295737-100295756 MsG0380017749.01.T01:intron 55.0%
! GGTCTCATCACTGGCCTCAA+AGG + Chr3:100295459-100295478 MsG0380017749.01.T01:CDS 55.0%
ACAGACGTGGGTCATCGTGC+TGG + Chr3:100295640-100295659 MsG0380017749.01.T01:CDS 60.0%
AGGTGGCTGATCGTGTCCAG+AGG + Chr3:100295406-100295425 MsG0380017749.01.T01:CDS 60.0%
GACCTGGTCGGTGGTTCGCA+TGG + Chr3:100295548-100295567 MsG0380017749.01.T01:CDS 65.0%
GTCGGTGGTTCGCATGGCAG+CGG + Chr3:100295554-100295573 MsG0380017749.01.T01:CDS 65.0%
TGCCATGCGAACCACCGACC+AGG - Chr3:100295553-100295572 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 100293298 100296265 100293298 ID=MsG0380017749.01;Name=MsG0380017749.01
Chr3 mRNA 100293298 100296265 100293298 ID=MsG0380017749.01.T01;Parent=MsG0380017749.01;Name=MsG0380017749.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0.66|0.75|1|0.66|0.75|4|216|331
Chr3 exon 100293298 100293364 100293298 ID=MsG0380017749.01.T01:exon:35755;Parent=MsG0380017749.01.T01
Chr3 exon 100293609 100293977 100293609 ID=MsG0380017749.01.T01:exon:35756;Parent=MsG0380017749.01.T01
Chr3 exon 100295346 100295686 100295346 ID=MsG0380017749.01.T01:exon:35757;Parent=MsG0380017749.01.T01
Chr3 exon 100295831 100296265 100295831 ID=MsG0380017749.01.T01:exon:35758;Parent=MsG0380017749.01.T01
Chr3 CDS 100293298 100293364 100293298 ID=MsG0380017749.01.T01:cds;Parent=MsG0380017749.01.T01
Chr3 CDS 100293609 100293977 100293609 ID=MsG0380017749.01.T01:cds;Parent=MsG0380017749.01.T01
Chr3 CDS 100295346 100295686 100295346 ID=MsG0380017749.01.T01:cds;Parent=MsG0380017749.01.T01
Chr3 CDS 100295831 100296049 100295831 ID=MsG0380017749.01.T01:cds;Parent=MsG0380017749.01.T01
Chr3 three_prime_UTR 100296050 100296265 100296050 ID=MsG0380017749.01.T01:three_prime_utr;Parent=MsG0380017749.01.T01
Gene Sequence

>MsG0380017749.01.T01

ATGGAACTTCAAACTCTTTGTTGCGCTCTTCCCTCTCAACCACTTCACTTCTCCAAACATCGTGTCACATTCAAACAGGTGATTAAAGAACCTTCATTTTTGGGATTGAAAAAATTTAAAAGGACTATTACACATGCTTCTGATTCCAATGTAAATGGTGCATTGGAAGTTGAACTGAAACAAAGTTCTAGTGTTCCTGTTAATGGGGGTTATAACGGGTTGGAACCATTTCATGGTAAATCAGGTTCTGTCTCTTTTTATGGGTTGACACACCAATCTGTAGAAGAAGGGAAGTTGGTATCTGCTCCGTTCAAACAAGAGGAGAGCTCTTACTTATGGGTTTTGGCTCCTGTTGCATTCATATCATCTTTGATTCTGCCACAGTTCTTCGTTGGCACTGTTGTTGCGGCTTTCTTTAATGATTTGATTTTAAAAGATATTGTGACTTCATTCTCTTCCGAGGCACTGTTCTATGTCGGACTTGCAACATTTTTGCAGGTGGCTGATCGTGTCCAGAGGCCATATTTACAGTACAGCTCTAAAAGATGGGGTCTCATCACTGGCCTCAAAGGATATATAACCTCTGCTTTCCTCACAACGGGTTTGAAAATTGCCGTTCCTCTTCTTCTTTTGTATGTGACCTGGTCGGTGGTTCGCATGGCAGCGGTTGTTGCTATAGCTCCCTTTTTAGTTGGCTGTGCTGCTCAATTTGCATTTGAGAGACATTTGGACAGACGTGGGTCATCGTGCTGGCCTCTAGTCCCTATTATATTTGAGGTATACAGATTGTATCAGCTAACAAAAGCAGCCAATTTTGCCGAGAAGTTGATGTACTCTATGAAGGGGCTACCTGCGTCACCTGAAGTACTAGAACGAAGTGGAGCTTTGTTTGGAATGATGGTGATTTTCCAGTTACTGGGTATTGTATGCATTTGGTCATTGATGACGTTTCTCTTGAGGCTATTCCCTTCTAGGCCAGTAGCAGAAAATTACTGA
Protein sequence

>MsG0380017749.01.T01

MELQTLCCALPSQPLHFSKHRVTFKQVIKEPSFLGLKKFKRTITHASDSNVNGALEVELKQSSSVPVNGGYNGLEPFHGKSGSVSFYGLTHQSVEEGKLVSAPFKQEESSYLWVLAPVAFISSLILPQFFVGTVVAAFFNDLILKDIVTSFSSEALFYVGLATFLQVADRVQRPYLQYSSKRWGLITGLKGYITSAFLTTGLKIAVPLLLLYVTWSVVRMAAVVAIAPFLVGCAAQFAFERHLDRRGSSCWPLVPIIFEVYRLYQLTKAANFAEKLMYSMKGLPASPEVLERSGALFGMMVIFQLLGIVCIWSLMTFLLRLFPSRPVAENY*