AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004750.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004750.01.T01 MTR_1g086000 99.065 107 1 0 1 107 37 143 1.70e-75 219
MsG0180004750.01.T01 MTR_6g060170 40.260 77 46 0 6 82 3 79 7.36e-17 70.1
MsG0180004750.01.T01 MTR_1g076840 30.952 84 58 0 3 86 34 117 3.93e-13 61.2
MsG0180004750.01.T01 MTR_6g059920 32.258 93 63 0 9 101 36 128 7.10e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004750.01.T01 AT2G32650 80.189 106 21 0 1 106 34 139 3.44e-61 183
MsG0180004750.01.T01 AT2G32650 80.189 106 21 0 1 106 34 139 3.44e-61 183
MsG0180004750.01.T01 AT2G32180 80.189 106 21 0 1 106 34 139 3.52e-61 183
MsG0180004750.01.T01 AT2G32180 80.189 106 21 0 1 106 34 139 3.52e-61 183
MsG0180004750.01.T01 AT4G10290 35.065 77 47 1 9 82 16 92 1.45e-11 57.0
MsG0180004750.01.T01 AT4G10300 29.762 84 59 0 3 86 36 119 6.97e-11 55.5

Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTCATGCAATTTGTTGC+CGG 0.346254 1:-82119032 MsG0180004750.01.T01:CDS
GAGGCTGATCTTTGGTTCAA+TGG 0.368609 1:-82118981 MsG0180004750.01.T01:CDS
CTGCCAATCCCATGGCAATC+TGG 0.379483 1:+82119115 None:intergenic
ATGTTTCAAGATGGTCAGTA+TGG 0.381328 1:-82119151 MsG0180004750.01.T01:CDS
AGTGGTTTGAGGCTGATCTT+TGG 0.382114 1:-82118989 MsG0180004750.01.T01:CDS
CGTTATAGTTTCCGAGCATA+TGG 0.389309 1:-82118945 MsG0180004750.01.T01:CDS
ATGAAACGCTTGCTCCCTTC+AGG 0.397604 1:+82119047 None:intergenic
TTGTTGCCGGAGATTTAATC+CGG 0.479684 1:-82119019 MsG0180004750.01.T01:CDS
CAATGTGAAGGTAGAAAGAA+AGG 0.481006 1:-82119201 MsG0180004750.01.T01:CDS
CCTCAAACCACTTCGGATAC+CGG 0.486692 1:+82119000 None:intergenic
AATCCAGATTGCCATGGGAT+TGG 0.487823 1:-82119118 MsG0180004750.01.T01:CDS
CGGATACCGGATTAAATCTC+CGG 0.495277 1:+82119013 None:intergenic
CAGATTGCCATGGGATTGGC+AGG 0.499106 1:-82119114 MsG0180004750.01.T01:CDS
GGATTGGCAGGTAGACCAAC+TGG 0.504664 1:-82119102 MsG0180004750.01.T01:CDS
CACATTGTATAATTCTTCCA+AGG 0.523416 1:+82119217 None:intergenic
AAACTATAACGTTCTTGATA+CGG 0.530071 1:+82118957 None:intergenic
AATGAAGATAGAGAAATCCT+TGG 0.554986 1:-82119234 MsG0180004750.01.T01:CDS
AGATGGTCAGTATGGAAGAC+CGG 0.563715 1:-82119143 MsG0180004750.01.T01:CDS
CTCAGCTTGATGTTTCAAGA+TGG 0.565626 1:-82119160 MsG0180004750.01.T01:CDS
CCGGTATCCGAAGTGGTTTG+AGG 0.566818 1:-82119000 MsG0180004750.01.T01:CDS
AATAATCATCACCATATGCT+CGG 0.568249 1:+82118934 None:intergenic
CGGAAAATCCAGATTGCCAT+GGG 0.572454 1:-82119123 MsG0180004750.01.T01:CDS
CCGGAAAATCCAGATTGCCA+TGG 0.585607 1:-82119124 MsG0180004750.01.T01:CDS
CTTCTTCTATGTACACCAGT+TGG 0.594279 1:+82119087 None:intergenic
CAACTGGTGTACATAGAAGA+AGG 0.600561 1:-82119086 MsG0180004750.01.T01:CDS
AAGTGAGAGTTGTTCCTGAA+GGG 0.600643 1:-82119061 MsG0180004750.01.T01:CDS
GAAGTGAGAGTTGTTCCTGA+AGG 0.600726 1:-82119062 MsG0180004750.01.T01:CDS
TGGTCTACCTGCCAATCCCA+TGG 0.603266 1:+82119107 None:intergenic
GGAAGAATTATACAATGTGA+AGG 0.609686 1:-82119213 MsG0180004750.01.T01:CDS
ATTTAATCCGGTATCCGAAG+TGG 0.624736 1:-82119007 MsG0180004750.01.T01:CDS
AGATCAGCCTCAAACCACTT+CGG 0.631094 1:+82118993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACTATAACGTTCTTGATA+CGG + Chr1:82119211-82119230 None:intergenic 25.0%
AATAATCATCACCATATGCT+CGG + Chr1:82119234-82119253 None:intergenic 30.0%
CACATTGTATAATTCTTCCA+AGG + Chr1:82118951-82118970 None:intergenic 30.0%
GGAAGAATTATACAATGTGA+AGG - Chr1:82118952-82118971 MsG0180004750.01.T01:CDS 30.0%
ATGTTTCAAGATGGTCAGTA+TGG - Chr1:82119014-82119033 MsG0180004750.01.T01:CDS 35.0%
CAATGTGAAGGTAGAAAGAA+AGG - Chr1:82118964-82118983 MsG0180004750.01.T01:CDS 35.0%
AAGTGAGAGTTGTTCCTGAA+GGG - Chr1:82119104-82119123 MsG0180004750.01.T01:CDS 40.0%
ATTTAATCCGGTATCCGAAG+TGG - Chr1:82119158-82119177 MsG0180004750.01.T01:CDS 40.0%
CAACTGGTGTACATAGAAGA+AGG - Chr1:82119079-82119098 MsG0180004750.01.T01:CDS 40.0%
CGTTATAGTTTCCGAGCATA+TGG - Chr1:82119220-82119239 MsG0180004750.01.T01:CDS 40.0%
CTCAGCTTGATGTTTCAAGA+TGG - Chr1:82119005-82119024 MsG0180004750.01.T01:CDS 40.0%
CTTCTTCTATGTACACCAGT+TGG + Chr1:82119081-82119100 None:intergenic 40.0%
TTGTTGCCGGAGATTTAATC+CGG - Chr1:82119146-82119165 MsG0180004750.01.T01:CDS 40.0%
!! CGTTTCATGCAATTTGTTGC+CGG - Chr1:82119133-82119152 MsG0180004750.01.T01:CDS 40.0%
!!! TCAAGCTGAGCTAGTTTTTC+AGG + Chr1:82118995-82119014 None:intergenic 40.0%
AATCCAGATTGCCATGGGAT+TGG - Chr1:82119047-82119066 MsG0180004750.01.T01:CDS 45.0%
AGATCAGCCTCAAACCACTT+CGG + Chr1:82119175-82119194 None:intergenic 45.0%
AGATGGTCAGTATGGAAGAC+CGG - Chr1:82119022-82119041 MsG0180004750.01.T01:CDS 45.0%
CGGAAAATCCAGATTGCCAT+GGG - Chr1:82119042-82119061 MsG0180004750.01.T01:CDS 45.0%
CGGATACCGGATTAAATCTC+CGG + Chr1:82119155-82119174 None:intergenic 45.0%
GAAGTGAGAGTTGTTCCTGA+AGG - Chr1:82119103-82119122 MsG0180004750.01.T01:CDS 45.0%
! AGTGGTTTGAGGCTGATCTT+TGG - Chr1:82119176-82119195 MsG0180004750.01.T01:CDS 45.0%
! CCATGGCAATCTGGATTTTC+CGG + Chr1:82119044-82119063 None:intergenic 45.0%
! GAGGCTGATCTTTGGTTCAA+TGG - Chr1:82119184-82119203 MsG0180004750.01.T01:CDS 45.0%
ATGAAACGCTTGCTCCCTTC+AGG + Chr1:82119121-82119140 None:intergenic 50.0%
CCGGAAAATCCAGATTGCCA+TGG - Chr1:82119041-82119060 MsG0180004750.01.T01:CDS 50.0%
CCTCAAACCACTTCGGATAC+CGG + Chr1:82119168-82119187 None:intergenic 50.0%
CCGGTATCCGAAGTGGTTTG+AGG - Chr1:82119165-82119184 MsG0180004750.01.T01:CDS 55.0%
CTGCCAATCCCATGGCAATC+TGG + Chr1:82119053-82119072 None:intergenic 55.0%
TGGTCTACCTGCCAATCCCA+TGG + Chr1:82119061-82119080 None:intergenic 55.0%
! CAGATTGCCATGGGATTGGC+AGG - Chr1:82119051-82119070 MsG0180004750.01.T01:CDS 55.0%
! GGATTGGCAGGTAGACCAAC+TGG - Chr1:82119063-82119082 MsG0180004750.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82118932 82119255 82118932 ID=MsG0180004750.01;Name=MsG0180004750.01
Chr1 mRNA 82118932 82119255 82118932 ID=MsG0180004750.01.T01;Parent=MsG0180004750.01;Name=MsG0180004750.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|107
Chr1 exon 82118932 82119255 82118932 ID=MsG0180004750.01.T01:exon:23385;Parent=MsG0180004750.01.T01
Chr1 CDS 82118932 82119255 82118932 ID=MsG0180004750.01.T01:cds;Parent=MsG0180004750.01.T01
Gene Sequence

>MsG0180004750.01.T01

ATGAAGATAGAGAAATCCTTGGAAGAATTATACAATGTGAAGGTAGAAAGAAAGGTGTCACCTGAAAAACTAGCTCAGCTTGATGTTTCAAGATGGTCAGTATGGAAGACCGGAAAATCCAGATTGCCATGGGATTGGCAGGTAGACCAACTGGTGTACATAGAAGAAGGTGAAGTGAGAGTTGTTCCTGAAGGGAGCAAGCGTTTCATGCAATTTGTTGCCGGAGATTTAATCCGGTATCCGAAGTGGTTTGAGGCTGATCTTTGGTTCAATGGTCCGTATCAAGAACGTTATAGTTTCCGAGCATATGGTGATGATTATTGA

Protein sequence

>MsG0180004750.01.T01

MKIEKSLEELYNVKVERKVSPEKLAQLDVSRWSVWKTGKSRLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFVAGDLIRYPKWFEADLWFNGPYQERYSFRAYGDDY*