AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380018052.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018052.01.T01 MTR_3g117890 98.718 156 2 0 1 156 1 156 3.26e-116 325
MsG0380018052.01.T01 MTR_4g073040 79.245 159 27 3 1 156 1 156 2.24e-88 254
MsG0380018052.01.T01 MTR_5g022620 61.491 161 53 3 1 156 1 157 1.02e-65 197
MsG0380018052.01.T01 MTR_1g030630 53.205 156 64 3 1 156 1 147 1.64e-53 166
MsG0380018052.01.T01 MTR_8g091420 51.613 155 69 3 2 156 5 153 5.52e-49 155
MsG0380018052.01.T01 MTR_3g087770 51.923 156 66 4 2 156 5 152 6.44e-49 155
MsG0380018052.01.T01 MTR_5g025150 47.436 156 71 3 1 156 1 145 1.61e-45 146
MsG0380018052.01.T01 MTR_6g003990 45.062 162 76 3 1 156 1 155 6.85e-45 145
MsG0380018052.01.T01 MTR_7g083060 48.077 156 71 3 1 156 1 146 7.89e-45 144
MsG0380018052.01.T01 MTR_3g073670 47.742 155 71 3 2 156 5 149 2.90e-42 138
MsG0380018052.01.T01 MTR_6g003990 46.341 123 61 1 34 156 1 118 3.89e-36 121
MsG0380018052.01.T01 MTR_5g055020 37.821 156 88 3 1 156 1 147 1.23e-34 118
MsG0380018052.01.T01 MTR_1g078090 38.686 137 76 4 25 156 52 185 2.87e-24 93.2
MsG0380018052.01.T01 MTR_6g026890 36.029 136 81 4 25 156 15 148 4.08e-23 89.4
MsG0380018052.01.T01 MTR_2g091235 36.429 140 77 3 28 156 4 142 4.26e-22 86.3
MsG0380018052.01.T01 MTR_6g045203 36.364 143 70 6 28 156 5 140 5.05e-18 75.9
MsG0380018052.01.T01 MTR_7g013660 48.485 66 33 1 26 91 4 68 3.92e-14 65.5
MsG0380018052.01.T01 MTR_3g435930 34.586 133 74 3 26 156 116 237 4.24e-14 67.4
MsG0380018052.01.T01 MTR_1g083310 36.765 68 43 0 25 92 14 81 2.56e-12 63.5
MsG0380018052.01.T01 MTR_1g092670 45.455 66 35 1 26 91 4 68 4.57e-12 58.9
MsG0380018052.01.T01 MTR_7g108560 36.765 68 42 1 24 91 20 86 7.12e-12 62.0
MsG0380018052.01.T01 MTR_7g113110 42.857 63 35 1 30 92 37 98 1.02e-11 62.0
MsG0380018052.01.T01 MTR_3g010130 55.769 52 21 2 1 52 1 50 2.53e-11 56.6
MsG0380018052.01.T01 MTR_1g107565 39.706 68 40 1 24 91 11 77 3.72e-11 60.5
MsG0380018052.01.T01 MTR_3g067750 41.538 65 37 1 26 90 12 75 4.95e-11 58.9
MsG0380018052.01.T01 MTR_7g100450 46.269 67 34 1 26 92 209 273 8.54e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018052.01.T01 AT4G38580 76.282 156 34 1 1 156 1 153 1.11e-84 245
MsG0380018052.01.T01 AT4G38580 76.282 156 34 1 1 156 1 153 1.11e-84 245
MsG0380018052.01.T01 AT4G35060 70.701 157 41 3 1 156 1 153 1.89e-76 224
MsG0380018052.01.T01 AT5G66110 66.667 138 44 1 19 156 12 147 2.19e-66 199
MsG0380018052.01.T01 AT5G66110 66.667 138 44 1 19 156 12 147 2.19e-66 199
MsG0380018052.01.T01 AT5G66110 65.854 123 40 1 34 156 1 121 1.48e-58 178
MsG0380018052.01.T01 AT1G71050 56.329 158 61 4 1 156 1 152 9.64e-54 167
MsG0380018052.01.T01 AT1G22990 49.057 159 71 2 1 156 1 152 2.00e-50 159
MsG0380018052.01.T01 AT1G22990 46.795 156 74 2 1 156 1 147 2.34e-47 150
MsG0380018052.01.T01 AT4G39700 48.734 158 74 4 2 156 5 158 1.24e-44 144
MsG0380018052.01.T01 AT5G17450 45.860 157 76 3 1 156 1 149 1.48e-42 139
MsG0380018052.01.T01 AT4G08570 44.231 156 76 4 2 156 5 150 3.28e-40 132
MsG0380018052.01.T01 AT5G17450 43.396 106 51 3 52 156 8 105 1.51e-25 94.4
MsG0380018052.01.T01 AT1G06330 38.000 150 71 6 27 156 3 150 4.89e-24 91.7
MsG0380018052.01.T01 AT2G18196 33.824 136 84 4 25 156 45 178 4.05e-20 82.4
MsG0380018052.01.T01 AT4G10465 33.566 143 89 3 18 156 43 183 1.80e-19 80.9
MsG0380018052.01.T01 AT3G56891 32.544 169 89 6 10 156 1 166 1.57e-18 77.8
MsG0380018052.01.T01 AT2G18196 33.071 127 79 4 34 156 1 125 2.37e-16 71.2
MsG0380018052.01.T01 AT3G56891 33.103 145 73 5 34 156 1 143 3.69e-16 71.2
MsG0380018052.01.T01 AT1G29100 33.974 156 65 4 27 156 4 147 1.26e-15 69.7
MsG0380018052.01.T01 AT1G29100 33.333 153 64 4 30 156 1 141 3.31e-14 65.9
MsG0380018052.01.T01 AT1G56210 39.744 78 46 1 25 102 39 115 9.06e-13 65.1
MsG0380018052.01.T01 AT1G66240 43.939 66 36 1 26 91 33 97 1.20e-12 60.8
MsG0380018052.01.T01 AT3G56240 47.761 67 34 1 26 92 3 68 2.19e-12 60.8
MsG0380018052.01.T01 AT3G56240 47.761 67 34 1 26 92 3 68 2.19e-12 60.8
MsG0380018052.01.T01 AT3G02960 28.571 133 85 2 24 156 124 246 4.53e-12 62.4
MsG0380018052.01.T01 AT5G27690 39.130 69 41 1 24 92 28 95 1.74e-11 61.2
MsG0380018052.01.T01 AT3G06130 39.706 68 40 1 24 91 8 74 4.39e-11 60.1

Find 47 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACACCGCAAACAATTTC+AGG 0.262177 3:-103765936 MsG0380018052.01.T01:intron
AGCACACAAGGTCACTGTTC+AGG 0.319616 3:-103765735 MsG0380018052.01.T01:CDS
TCGTACGTACCTTGTGCATA+TGG 0.340646 3:+103765614 None:intergenic
TACGACAACAATCAGTATTC+TGG 0.342642 3:-103765557 MsG0380018052.01.T01:CDS
ATACGCGCCACCACTTTGTT+AGG 0.355486 3:+103765701 None:intergenic
TCGCTCACCGCACTGGTAAG+AGG 0.420324 3:-103765679 MsG0380018052.01.T01:CDS
CTTTCCTATCAATATCAACT+TGG 0.425944 3:+103765756 None:intergenic
GTAGTTGTTTCTAACATAAC+CGG 0.435319 3:+103765577 None:intergenic
AAGAGATAATATGGGTGCTC+TGG 0.436844 3:-103766002 None:intergenic
GGGTGTGCAACGACGTCGTA+TGG 0.444075 3:+103765635 None:intergenic
CTATTACCTGAAATTGTTTG+CGG 0.461292 3:+103765930 None:intergenic
GAAATTGTTTGCGGTGTTTG+TGG 0.465422 3:+103765939 None:intergenic
ACGACGTCGTATGGAACATA+TGG 0.471597 3:+103765644 None:intergenic
TCAGTATTCTGGATCCCATC+TGG 0.473879 3:-103765546 MsG0380018052.01.T01:CDS
TGAACCAAGTTGATATTGAT+AGG 0.475748 3:-103765760 MsG0380018052.01.T01:CDS
AAATTGTTTGCGGTGTTTGT+GGG 0.476948 3:+103765940 None:intergenic
GCACACAAGGTCACTGTTCA+GGG 0.478112 3:-103765734 MsG0380018052.01.T01:CDS
TACGACAAGAAAGCACCTTC+CGG 0.490150 3:-103765596 MsG0380018052.01.T01:CDS
ATGATATCACATGACAGAAC+AGG 0.494670 3:+103765463 None:intergenic
CTGGTAAGAGGGCTGAGATA+TGG 0.502117 3:-103765667 MsG0380018052.01.T01:CDS
CGTACGTACCTTGTGCATAT+GGG 0.507821 3:+103765615 None:intergenic
GTGTTTGTGGGAACTTCCAC+CGG 0.512215 3:+103765952 None:intergenic
TGGAACTAGCACGCGCCAGA+TGG 0.513296 3:+103765531 None:intergenic
GCGCGTATCGCTCACCGCAC+TGG 0.514553 3:-103765686 MsG0380018052.01.T01:CDS
CGCTCACCGCACTGGTAAGA+GGG 0.517783 3:-103765678 MsG0380018052.01.T01:CDS
TCTGAGCTATTTGACTGCTC+CGG 0.534149 3:-103765971 MsG0380018052.01.T01:CDS
AAAGTGAGAAGGTCGGTGGA+AGG 0.544215 3:-103765794 MsG0380018052.01.T01:CDS
GGTGGAAGTGAAAGTGAAGA+TGG 0.547212 3:-103765837 MsG0380018052.01.T01:CDS
GAGCTATTTGACTGCTCCGG+TGG 0.554467 3:-103765968 MsG0380018052.01.T01:CDS
GGAACTAGCACGCGCCAGAT+GGG 0.557432 3:+103765532 None:intergenic
AGGTCGGTGGAAGGAATGAA+AGG 0.586836 3:-103765785 MsG0380018052.01.T01:CDS
GAGAAAAGTGAGAAGGTCGG+TGG 0.602080 3:-103765798 MsG0380018052.01.T01:CDS
AGCGGTGGTGTAGCGAACTT+CGG 0.605621 3:+103765508 None:intergenic
TCACATGACAGAACAGGCGG+TGG 0.625825 3:+103765469 None:intergenic
CGAGAGAAAAGTGAGAAGGT+CGG 0.626298 3:-103765801 MsG0380018052.01.T01:CDS
TATTGATAGGAAAGCACACA+AGG 0.639331 3:-103765747 MsG0380018052.01.T01:CDS
GATGCGAGAGAAAAGTGAGA+AGG 0.650873 3:-103765805 MsG0380018052.01.T01:CDS
CACATGACAGAACAGGCGGT+GGG 0.664346 3:+103765470 None:intergenic
TTGTTTCTAACATAACCGGA+AGG 0.675813 3:+103765581 None:intergenic
TGTTGAACCTAACAAAGTGG+TGG 0.684270 3:-103765708 MsG0380018052.01.T01:CDS
CACACAAGGTCACTGTTCAG+GGG 0.691299 3:-103765733 MsG0380018052.01.T01:CDS
GGTGGTGTAGCGAACTTCGG+TGG 0.699721 3:+103765511 None:intergenic
ATATCACATGACAGAACAGG+CGG 0.726418 3:+103765466 None:intergenic
GTTGCACACCCATATGCACA+AGG 0.727359 3:-103765623 MsG0380018052.01.T01:CDS
GTATGTTGAACCTAACAAAG+TGG 0.728726 3:-103765711 MsG0380018052.01.T01:CDS
TCTCAGCCCTCTTACCAGTG+CGG 0.734386 3:+103765672 None:intergenic
AAAGTGAAGATGGACTGCGA+AGG 0.769260 3:-103765827 MsG0380018052.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAACATATGAATATGAA+TGG + Chr3:103765564-103765583 None:intergenic 15.0%
!! TATATATATATATGTACAGA+CGG - Chr3:103765603-103765622 MsG0380018052.01.T01:CDS 15.0%
! ACATATGAATATGAATGGAA+TGG + Chr3:103765559-103765578 None:intergenic 25.0%
ATATATATATGTACAGACGG+TGG - Chr3:103765606-103765625 MsG0380018052.01.T01:CDS 30.0%
CTATTACCTGAAATTGTTTG+CGG + Chr3:103765534-103765553 None:intergenic 30.0%
CTTTCCTATCAATATCAACT+TGG + Chr3:103765708-103765727 None:intergenic 30.0%
!! GTAGTTGTTTCTAACATAAC+CGG + Chr3:103765887-103765906 None:intergenic 30.0%
!! TGAACCAAGTTGATATTGAT+AGG - Chr3:103765701-103765720 MsG0380018052.01.T01:CDS 30.0%
AAATTGTTTGCGGTGTTTGT+GGG + Chr3:103765524-103765543 None:intergenic 35.0%
GTATGTTGAACCTAACAAAG+TGG - Chr3:103765750-103765769 MsG0380018052.01.T01:CDS 35.0%
TACGACAACAATCAGTATTC+TGG - Chr3:103765904-103765923 MsG0380018052.01.T01:intron 35.0%
! TATTGATAGGAAAGCACACA+AGG - Chr3:103765714-103765733 MsG0380018052.01.T01:CDS 35.0%
!! TTGTTTCTAACATAACCGGA+AGG + Chr3:103765883-103765902 None:intergenic 35.0%
CAAACACCGCAAACAATTTC+AGG - Chr3:103765525-103765544 MsG0380018052.01.T01:CDS 40.0%
GAAATTGTTTGCGGTGTTTG+TGG + Chr3:103765525-103765544 None:intergenic 40.0%
TGTTGAACCTAACAAAGTGG+TGG - Chr3:103765753-103765772 MsG0380018052.01.T01:CDS 40.0%
ACGACGTCGTATGGAACATA+TGG + Chr3:103765820-103765839 None:intergenic 45.0%
CGAGAGAAAAGTGAGAAGGT+CGG - Chr3:103765660-103765679 MsG0380018052.01.T01:CDS 45.0%
CGTACGTACCTTGTGCATAT+GGG + Chr3:103765849-103765868 None:intergenic 45.0%
GATGCGAGAGAAAAGTGAGA+AGG - Chr3:103765656-103765675 MsG0380018052.01.T01:CDS 45.0%
GGTGGAAGTGAAAGTGAAGA+TGG - Chr3:103765624-103765643 MsG0380018052.01.T01:CDS 45.0%
TCAGTATTCTGGATCCCATC+TGG - Chr3:103765915-103765934 MsG0380018052.01.T01:intron 45.0%
TCGTACGTACCTTGTGCATA+TGG + Chr3:103765850-103765869 None:intergenic 45.0%
TCTGAGCTATTTGACTGCTC+CGG - Chr3:103765490-103765509 MsG0380018052.01.T01:CDS 45.0%
!! AAAGTGAAGATGGACTGCGA+AGG - Chr3:103765634-103765653 MsG0380018052.01.T01:CDS 45.0%
!! TACGACAAGAAAGCACCTTC+CGG - Chr3:103765865-103765884 MsG0380018052.01.T01:intron 45.0%
AAAGTGAGAAGGTCGGTGGA+AGG - Chr3:103765667-103765686 MsG0380018052.01.T01:CDS 50.0%
AGCACACAAGGTCACTGTTC+AGG - Chr3:103765726-103765745 MsG0380018052.01.T01:CDS 50.0%
AGGTCGGTGGAAGGAATGAA+AGG - Chr3:103765676-103765695 MsG0380018052.01.T01:CDS 50.0%
ATACGCGCCACCACTTTGTT+AGG + Chr3:103765763-103765782 None:intergenic 50.0%
CACACAAGGTCACTGTTCAG+GGG - Chr3:103765728-103765747 MsG0380018052.01.T01:CDS 50.0%
CTGGTAAGAGGGCTGAGATA+TGG - Chr3:103765794-103765813 MsG0380018052.01.T01:CDS 50.0%
GAGAAAAGTGAGAAGGTCGG+TGG - Chr3:103765663-103765682 MsG0380018052.01.T01:CDS 50.0%
GCACACAAGGTCACTGTTCA+GGG - Chr3:103765727-103765746 MsG0380018052.01.T01:CDS 50.0%
GTGTTTGTGGGAACTTCCAC+CGG + Chr3:103765512-103765531 None:intergenic 50.0%
GTTGCACACCCATATGCACA+AGG - Chr3:103765838-103765857 MsG0380018052.01.T01:CDS 50.0%
! ATTTTCGTCGCTGAAAGCGG+TGG + Chr3:103765971-103765990 None:intergenic 50.0%
! GGGATTTTCGTCGCTGAAAG+CGG + Chr3:103765974-103765993 None:intergenic 50.0%
AGCGGTGGTGTAGCGAACTT+CGG + Chr3:103765956-103765975 None:intergenic 55.0%
CACATGACAGAACAGGCGGT+GGG + Chr3:103765994-103766013 None:intergenic 55.0%
GAGCTATTTGACTGCTCCGG+TGG - Chr3:103765493-103765512 MsG0380018052.01.T01:CDS 55.0%
TCACATGACAGAACAGGCGG+TGG + Chr3:103765995-103766014 None:intergenic 55.0%
TCTCAGCCCTCTTACCAGTG+CGG + Chr3:103765792-103765811 None:intergenic 55.0%
GGAACTAGCACGCGCCAGAT+GGG + Chr3:103765932-103765951 None:intergenic 60.0%
GGGTGTGCAACGACGTCGTA+TGG + Chr3:103765829-103765848 None:intergenic 60.0%
GGTGGTGTAGCGAACTTCGG+TGG + Chr3:103765953-103765972 None:intergenic 60.0%
TGGAACTAGCACGCGCCAGA+TGG + Chr3:103765933-103765952 None:intergenic 60.0%
! CGCTCACCGCACTGGTAAGA+GGG - Chr3:103765783-103765802 MsG0380018052.01.T01:CDS 60.0%
! TCGCTCACCGCACTGGTAAG+AGG - Chr3:103765782-103765801 MsG0380018052.01.T01:CDS 60.0%
GCGCGTATCGCTCACCGCAC+TGG - Chr3:103765775-103765794 MsG0380018052.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 103765469 103766014 103765469 ID=MsG0380018052.01;Name=MsG0380018052.01
Chr3 mRNA 103765469 103766014 103765469 ID=MsG0380018052.01.T01;Parent=MsG0380018052.01;Name=MsG0380018052.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|156
Chr3 exon 103765937 103766014 103765937 ID=MsG0380018052.01.T01:exon:30965;Parent=MsG0380018052.01.T01
Chr3 exon 103765469 103765861 103765469 ID=MsG0380018052.01.T01:exon:30964;Parent=MsG0380018052.01.T01
Chr3 CDS 103765937 103766014 103765937 ID=MsG0380018052.01.T01:cds;Parent=MsG0380018052.01.T01
Chr3 CDS 103765469 103765861 103765469 ID=MsG0380018052.01.T01:cds;Parent=MsG0380018052.01.T01
Gene Sequence

>MsG0380018052.01.T01

ATGGGTGCTCTGGATCACATTTCTGAGCTATTTGACTGCTCCGGTGGAAGTTCCCACAAACACCGCAAACAATTTCAGACGGTGGAAGTGAAAGTGAAGATGGACTGCGAAGGATGCGAGAGAAAAGTGAGAAGGTCGGTGGAAGGAATGAAAGGTGTGAACCAAGTTGATATTGATAGGAAAGCACACAAGGTCACTGTTCAGGGGTATGTTGAACCTAACAAAGTGGTGGCGCGTATCGCTCACCGCACTGGTAAGAGGGCTGAGATATGGCCATATGTTCCATACGACGTCGTTGCACACCCATATGCACAAGGTACGTACGACAAGAAAGCACCTTCCGGTTATGTTAGAAACAACTACGACAACAATCAGTATTCTGGATCCCATCTGGCGCGTGCTAGTTCCACCGAAGTTCGCTACACCACCGCTTTCAGCGACGAAAATCCCACCGCCTGTTCTGTCATGTGA

Protein sequence

>MsG0380018052.01.T01

MGALDHISELFDCSGGSSHKHRKQFQTVEVKVKMDCEGCERKVRRSVEGMKGVNQVDIDRKAHKVTVQGYVEPNKVVARIAHRTGKRAEIWPYVPYDVVAHPYAQGTYDKKAPSGYVRNNYDNNQYSGSHLARASSTEVRYTTAFSDENPTACSVM*