AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000023.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000023.01.T01 MTR_1g026078 88.043 92 3 2 1 84 68 159 7.14e-46 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000023.01.T01 AT1G76060 71.250 80 19 1 1 76 75 154 7.65e-35 116

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCACTCGGTTGCTTAT+CGG 0.353596 1:+443116 MsG0180000023.01.T01:CDS
ACGATGCCAAAGGCGTTCTA+TGG 0.381120 1:+443027 MsG0180000023.01.T01:CDS
AAGGATCGTCGCGGTGGCAG+TGG 0.428548 1:+443191 MsG0180000023.01.T01:CDS
ATCACGCCATAGAACGCCTT+TGG 0.477887 1:-443033 None:intergenic
TGCGAGGTTTGAGACGGATC+CGG 0.482393 1:+443091 MsG0180000023.01.T01:CDS
AGCAACCGAGTGACAATCTC+CGG 0.496816 1:-443110 None:intergenic
GATCGTCGCGGTGGCAGTGG+CGG 0.542143 1:+443194 MsG0180000023.01.T01:CDS
TTCACATGGCACGATGCCAA+AGG 0.565614 1:+443017 MsG0180000023.01.T01:CDS
TGAGCTTGCGAGGTTTGAGA+CGG 0.591164 1:+443085 MsG0180000023.01.T01:CDS
GTCACTCGGTTGCTTATCGG+TGG 0.599413 1:+443119 MsG0180000023.01.T01:CDS
GTAAAGAGTTTGAGCTTGCG+AGG 0.613271 1:+443075 MsG0180000023.01.T01:CDS
GTTGCTTATCGGTGGACGTG+AGG 0.631369 1:+443127 MsG0180000023.01.T01:CDS
CAACGCTATTGATAAGCTTG+CGG 0.647873 1:+443157 MsG0180000023.01.T01:CDS
ATTCAGAAGGATCGTCGCGG+TGG 0.657849 1:+443185 MsG0180000023.01.T01:CDS
GCTTGCGGAACAGATTCAGA+AGG 0.661253 1:+443172 MsG0180000023.01.T01:CDS
CGGATCCGGAGATTGTCACT+CGG 0.686306 1:+443105 MsG0180000023.01.T01:CDS
CAGATTCAGAAGGATCGTCG+CGG 0.756987 1:+443182 MsG0180000023.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTGTCACTCGGTTGCTTAT+CGG + Chr1:443116-443135 MsG0180000023.01.T01:CDS 40.0%
CAACGCTATTGATAAGCTTG+CGG + Chr1:443157-443176 MsG0180000023.01.T01:CDS 40.0%
! GTGCTTCACGATTTTTCACA+TGG + Chr1:443003-443022 MsG0180000023.01.T01:CDS 40.0%
GTAAAGAGTTTGAGCTTGCG+AGG + Chr1:443075-443094 MsG0180000023.01.T01:CDS 45.0%
AGCAACCGAGTGACAATCTC+CGG - Chr1:443113-443132 None:intergenic 50.0%
ATCACGCCATAGAACGCCTT+TGG - Chr1:443036-443055 None:intergenic 50.0%
CAGATTCAGAAGGATCGTCG+CGG + Chr1:443182-443201 MsG0180000023.01.T01:CDS 50.0%
GCTTGCGGAACAGATTCAGA+AGG + Chr1:443172-443191 MsG0180000023.01.T01:CDS 50.0%
TGAGCTTGCGAGGTTTGAGA+CGG + Chr1:443085-443104 MsG0180000023.01.T01:CDS 50.0%
TTCACATGGCACGATGCCAA+AGG + Chr1:443017-443036 MsG0180000023.01.T01:CDS 50.0%
!! ACGATGCCAAAGGCGTTCTA+TGG + Chr1:443027-443046 MsG0180000023.01.T01:CDS 50.0%
ATTCAGAAGGATCGTCGCGG+TGG + Chr1:443185-443204 MsG0180000023.01.T01:CDS 55.0%
CGGATCCGGAGATTGTCACT+CGG + Chr1:443105-443124 MsG0180000023.01.T01:CDS 55.0%
GTCACTCGGTTGCTTATCGG+TGG + Chr1:443119-443138 MsG0180000023.01.T01:CDS 55.0%
GTTGCTTATCGGTGGACGTG+AGG + Chr1:443127-443146 MsG0180000023.01.T01:CDS 55.0%
! TGCGAGGTTTGAGACGGATC+CGG + Chr1:443091-443110 MsG0180000023.01.T01:CDS 55.0%
AAGGATCGTCGCGGTGGCAG+TGG + Chr1:443191-443210 MsG0180000023.01.T01:CDS 65.0%
GATCGTCGCGGTGGCAGTGG+CGG + Chr1:443194-443213 MsG0180000023.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 442978 443232 442978 ID=MsG0180000023.01;Name=MsG0180000023.01
Chr1 mRNA 442978 443232 442978 ID=MsG0180000023.01.T01;Parent=MsG0180000023.01;Name=MsG0180000023.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|84
Chr1 exon 442978 443232 442978 ID=MsG0180000023.01.T01:exon:7855;Parent=MsG0180000023.01.T01
Chr1 CDS 442978 443232 442978 ID=MsG0180000023.01.T01:cds;Parent=MsG0180000023.01.T01
Gene Sequence

>MsG0180000023.01.T01

CTGAGTCTCTACAGAGACATTCTTCGTGCTTCACGATTTTTCACATGGCACGATGCCAAAGGCGTTCTATGGCGTGATCTTCTTAGAGATAGCGCTCGTAAAGAGTTTGAGCTTGCGAGGTTTGAGACGGATCCGGAGATTGTCACTCGGTTGCTTATCGGTGGACGTGAGGCTGTCAACAACGCTATTGATAAGCTTGCGGAACAGATTCAGAAGGATCGTCGCGGTGGCAGTGGCGGTTTTGATCAAACGTGA

Protein sequence

>MsG0180000023.01.T01

LSLYRDILRASRFFTWHDAKGVLWRDLLRDSARKEFELARFETDPEIVTRLLIGGREAVNNAIDKLAEQIQKDRRGGSGGFDQT*