Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019980.01.T01 | XP_013443594.1 | 100 | 74 | 0 | 0 | 1 | 74 | 90 | 163 | 2.29E-44 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019980.01.T01 | B9RW00 | 70.27 | 74 | 22 | 0 | 1 | 74 | 90 | 163 | 2.83E-32 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019980.01.T01 | A0A072TJ77 | 100.000 | 74 | 0 | 0 | 1 | 74 | 90 | 163 | 1.10e-44 | 150 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001079.01 | MsG0480019980.01 | 0.808384 | 3.180016e-50 | 1.815789e-47 |
| MsG0180002808.01 | MsG0480019980.01 | 0.833841 | 4.230578e-56 | 4.931775e-53 |
| MsG0180002935.01 | MsG0480019980.01 | 0.818619 | 1.783314e-52 | 1.341491e-49 |
| MsG0180003375.01 | MsG0480019980.01 | 0.814415 | 1.558846e-51 | 1.045379e-48 |
| MsG0180003482.01 | MsG0480019980.01 | 0.816603 | 5.078438e-52 | 3.614810e-49 |
| MsG0180005353.01 | MsG0480019980.01 | 0.818485 | 1.912996e-52 | 1.433831e-49 |
| MsG0180005757.01 | MsG0480019980.01 | 0.847107 | 1.425212e-59 | 2.506853e-56 |
| MsG0380014410.01 | MsG0480019980.01 | 0.804541 | 2.055523e-49 | 1.062636e-46 |
| MsG0380014734.01 | MsG0480019980.01 | 0.838328 | 3.069250e-57 | 4.096854e-54 |
| MsG0480019980.01 | MsG0480020722.01 | 0.829183 | 5.937029e-55 | 6.031504e-52 |
| MsG0480019980.01 | MsG0480022309.01 | 0.809160 | 2.170616e-50 | 1.265040e-47 |
| MsG0480019980.01 | MsG0480023322.01 | 0.826579 | 2.510992e-54 | 2.364166e-51 |
| MsG0480019980.01 | MsG0580025023.01 | 0.812984 | 3.219824e-51 | 2.076868e-48 |
| MsG0480019980.01 | MsG0580027036.01 | 0.828210 | 1.020505e-54 | 1.007658e-51 |
| MsG0480019980.01 | MsG0580028302.01 | 0.809028 | 2.316647e-50 | 1.345335e-47 |
| MsG0480019980.01 | MsG0680031464.01 | 0.803478 | 3.419432e-49 | 1.720156e-46 |
| MsG0480019980.01 | MsG0780040658.01 | 0.816842 | 4.489504e-52 | 3.216608e-49 |
| MsG0480019980.01 | MsG0780040878.01 | 0.812203 | 4.771638e-51 | 3.014777e-48 |
| MsG0480019980.01 | MsG0780040977.01 | 0.808943 | 2.415818e-50 | 1.399699e-47 |
| MsG0480019980.01 | MsG0780041180.01 | 0.800255 | 1.570272e-48 | 7.277739e-46 |
| MsG0480019980.01 | MsG0880043550.01 | 0.804765 | 1.845906e-49 | 9.598546e-47 |
| MsG0480019980.01 | MsG0880046463.01 | 0.855529 | 5.933265e-62 | 1.373967e-58 |
| MsG0480019980.01 | MsG0880047714.01 | 0.823209 | 1.566492e-53 | 1.340094e-50 |
| MsG0380016476.01 | MsG0480019980.01 | 0.803540 | 3.320437e-49 | 1.673057e-46 |
| MsG0480018440.01 | MsG0480019980.01 | 0.829966 | 3.830051e-55 | 3.980539e-52 |
| MsG0280006485.01 | MsG0480019980.01 | 0.815131 | 1.081805e-51 | 7.395638e-49 |
| MsG0280008659.01 | MsG0480019980.01 | 0.833357 | 5.588304e-56 | 6.420233e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019980.01.T01 | MTR_0004s0210 | 100.000 | 74 | 0 | 0 | 1 | 74 | 90 | 163 | 2.78e-48 | 150 |
| MsG0480019980.01.T01 | MTR_4g081880 | 50.000 | 60 | 30 | 0 | 2 | 61 | 121 | 180 | 2.61e-12 | 58.9 |
| MsG0480019980.01.T01 | MTR_0097s0070 | 43.333 | 60 | 34 | 0 | 9 | 68 | 133 | 192 | 2.42e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019980.01.T01 | AT1G03700 | 59.459 | 74 | 30 | 0 | 1 | 74 | 91 | 164 | 4.77e-29 | 101 |
| MsG0480019980.01.T01 | AT4G03540 | 62.162 | 74 | 28 | 0 | 1 | 74 | 91 | 164 | 5.71e-25 | 91.3 |
| MsG0480019980.01.T01 | AT5G44550 | 44.444 | 54 | 30 | 0 | 15 | 68 | 130 | 183 | 6.25e-11 | 55.5 |
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATGTGATTGCAAAATT+TGG | 0.163683 | 4:-31102648 | None:intergenic |
| GCTTTAGTCGCTAGTTTCAT+TGG | 0.393377 | 4:+31102674 | MsG0480019980.01.T01:CDS |
| CTCAAGCATCTCTGCAGCTT+TGG | 0.400682 | 4:+31102559 | MsG0480019980.01.T01:CDS |
| GTCATGTGATTGCAAAATTT+GGG | 0.430792 | 4:-31102647 | None:intergenic |
| GCTGCTTGGCTACCGATTTG+TGG | 0.463642 | 4:+31102617 | MsG0480019980.01.T01:CDS |
| ATGGAAACAATTATGCTGCT+TGG | 0.504739 | 4:+31102603 | MsG0480019980.01.T01:CDS |
| ATAGCTCAAGTGGCAAAGAA+TGG | 0.508607 | 4:+31102584 | MsG0480019980.01.T01:CDS |
| TTGGGAACTGAACCACAAAT+CGG | 0.511633 | 4:-31102629 | None:intergenic |
| AGGGTCAAGGACAGTATGAA+TGG | 0.516263 | 4:-31102729 | None:intergenic |
| TTCTCAAAAGGAGAGGGTCA+AGG | 0.544463 | 4:-31102742 | None:intergenic |
| AGCTTTGGCTATAGCTCAAG+TGG | 0.581117 | 4:+31102574 | MsG0480019980.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTCATGTGATTGCAAAATTT+GGG | - | Chr4:31102650-31102669 | None:intergenic | 30.0% | |
| TGTCATGTGATTGCAAAATT+TGG | - | Chr4:31102651-31102670 | None:intergenic | 30.0% | |
| ! | AAATTTTGCAATCACATGAC+AGG | + | Chr4:31102650-31102669 | MsG0480019980.01.T01:CDS | 30.0% |
| ! | AAGTTTTTCTCAAAAGGAGA+GGG | - | Chr4:31102751-31102770 | None:intergenic | 30.0% |
| ATGGAAACAATTATGCTGCT+TGG | + | Chr4:31102603-31102622 | MsG0480019980.01.T01:CDS | 35.0% | |
| ! | CAAGTTTTTCTCAAAAGGAG+AGG | - | Chr4:31102752-31102771 | None:intergenic | 35.0% |
| ATAGCTCAAGTGGCAAAGAA+TGG | + | Chr4:31102584-31102603 | MsG0480019980.01.T01:CDS | 40.0% | |
| TTGGGAACTGAACCACAAAT+CGG | - | Chr4:31102632-31102651 | None:intergenic | 40.0% | |
| ! | GCTTTAGTCGCTAGTTTCAT+TGG | + | Chr4:31102674-31102693 | MsG0480019980.01.T01:CDS | 40.0% |
| AGGGTCAAGGACAGTATGAA+TGG | - | Chr4:31102732-31102751 | None:intergenic | 45.0% | |
| TTCTCAAAAGGAGAGGGTCA+AGG | - | Chr4:31102745-31102764 | None:intergenic | 45.0% | |
| ! | AGCTTTGGCTATAGCTCAAG+TGG | + | Chr4:31102574-31102593 | MsG0480019980.01.T01:CDS | 45.0% |
| CTCAAGCATCTCTGCAGCTT+TGG | + | Chr4:31102559-31102578 | MsG0480019980.01.T01:CDS | 50.0% | |
| !! | GCTGCTTGGCTACCGATTTG+TGG | + | Chr4:31102617-31102636 | MsG0480019980.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 31102548 | 31102772 | 31102548 | ID=MsG0480019980.01;Name=MsG0480019980.01 |
| Chr4 | mRNA | 31102548 | 31102772 | 31102548 | ID=MsG0480019980.01.T01;Parent=MsG0480019980.01;Name=MsG0480019980.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|74 |
| Chr4 | exon | 31102548 | 31102772 | 31102548 | ID=MsG0480019980.01.T01:exon:10186;Parent=MsG0480019980.01.T01 |
| Chr4 | CDS | 31102548 | 31102772 | 31102548 | ID=MsG0480019980.01.T01:cds;Parent=MsG0480019980.01.T01 |
| Gene Sequence |
| Protein sequence |
