AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024270.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024270.01.T01 MTR_5g007880 89.474 133 13 1 34 165 2 134 1.86e-77 233
MsG0580024270.01.T01 MTR_5g007880 89.474 133 13 1 34 165 2 134 3.68e-77 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCACCAGTTCCAGTAAA+TGG 0.199349 5:+2786266 MsG0580024269.01.T01:CDS
CGTGAAACTGCAGAAGCTTT+TGG 0.202603 5:-2786236 MsG0580024270.01.T01:three_prime_UTR
TGTATTTGCTAACCTTTATT+AGG 0.261303 5:-2784799 MsG0580024270.01.T01:three_prime_UTR
GAAAATCTGCTCACAACTAA+TGG 0.271857 5:-2788048 MsG0580024270.01.T01:CDS
ACCTTGTTATTGTCCTCTTC+TGG 0.276965 5:-2785013 MsG0580024270.01.T01:three_prime_UTR
GTAGTCTTCTAAGTTGTATA+TGG 0.288003 5:-2784925 MsG0580024270.01.T01:three_prime_UTR
AACTGATGCCTGATAGAAAA+AGG 0.304850 5:+2785070 MsG0580024269.01.T01:intron
GTGTAAACTAACCCATTTAC+TGG 0.330313 5:-2786278 MsG0580024270.01.T01:three_prime_UTR
TACTATAATGCTTGTGCATT+TGG 0.335807 5:-2784947 MsG0580024270.01.T01:three_prime_UTR
TAAATTTGAATTACCTTTGT+TGG 0.343920 5:+2788946 None:intergenic
ACTTACTTATGGAACTCTTT+AGG 0.344380 5:+2787634 None:intergenic
AAGAAGTTCATGCAATATTT+CGG 0.347349 5:+2787854 None:intergenic
AAAATCTGCTCACAACTAAT+GGG 0.354040 5:-2788047 MsG0580024270.01.T01:CDS
TTGTACTACAAGTTGATTGC+TGG 0.357928 5:-2785195 MsG0580024270.01.T01:three_prime_UTR
ACATTTACTGTATAGTGTAT+AGG 0.365889 5:-2784847 MsG0580024270.01.T01:three_prime_UTR
AATATTGTGTTTATGATGAT+TGG 0.376132 5:-2789682 MsG0580024270.01.T01:CDS
ACTAACCCATTTACTGGAAC+TGG 0.376681 5:-2786272 MsG0580024270.01.T01:three_prime_UTR
CATTTACTGTATAGTGTATA+GGG 0.383314 5:-2784846 MsG0580024270.01.T01:three_prime_UTR
ACAGAAAGTTGGGCATATTC+TGG 0.385740 5:+2788081 None:intergenic
GAAACGTGCAATGGAGGCTT+TGG 0.387822 5:-2789152 MsG0580024270.01.T01:CDS
TCGGATGATGGACGACATAA+AGG 0.400815 5:+2788974 None:intergenic
AAATTTGAATTACCTTTGTT+GGG 0.407924 5:+2788947 None:intergenic
CATTTACAGTCACAGAAAGT+TGG 0.409130 5:+2788070 None:intergenic
AAACTAGATTCAAGAAAGGT+TGG 0.424013 5:-2785151 MsG0580024270.01.T01:three_prime_UTR
ATTTACAGTCACAGAAAGTT+GGG 0.425288 5:+2788071 None:intergenic
AAGATGCCATCTAAAACTTA+AGG 0.426832 5:+2785108 MsG0580024269.01.T01:intron
AAAGTTGTTGCTTAATCCAC+TGG 0.428214 5:-2787317 MsG0580024270.01.T01:three_prime_UTR
CAGTACAGTACCTTGTGAAA+TGG 0.436121 5:+2788244 None:intergenic
TCATTAGAAGTAGAGGGTGG+TGG 0.447897 5:+2789007 None:intergenic
TGCTGGAAAGCCACTCTGCT+TGG 0.452437 5:-2785178 MsG0580024270.01.T01:three_prime_UTR
TTATCAATGCTGCTGAATCA+AGG 0.456566 5:-2789174 MsG0580024270.01.T01:CDS
TTGTTCCAGGAATAATCAAT+TGG 0.458394 5:-2787183 MsG0580024270.01.T01:intron
AATTGCAGTTGTTCCGAAGA+AGG 0.466042 5:-2785227 MsG0580024270.01.T01:intron
TTCTTCATCTTCTCAAACAA+GGG 0.478707 5:-2787734 MsG0580024270.01.T01:three_prime_UTR
ACAAATACAGAACCCTTCTT+CGG 0.485262 5:+2785214 MsG0580024269.01.T01:intron
TGCCACCAGTTCCAGTAAAT+GGG 0.485264 5:+2786267 MsG0580024269.01.T01:CDS
CAATTGGCTCAATGTCTCCT+AGG 0.493446 5:-2787167 MsG0580024270.01.T01:three_prime_UTR
CAATTTAATTAGCTATTGGC+AGG 0.494801 5:-2784880 MsG0580024270.01.T01:three_prime_UTR
ATTACCTTTGTTGGGTGTAT+CGG 0.513719 5:+2788955 None:intergenic
TCTTTCCACATGTCATGCAT+TGG 0.514242 5:+2787346 None:intergenic
ATTACTTACACATGAATAGC+AGG 0.527926 5:+2789613 None:intergenic
TTGTTGGGTGTATCGGATGA+TGG 0.531290 5:+2788962 None:intergenic
GGCATCTTTCGTTTCACATG+AGG 0.532003 5:-2785093 MsG0580024270.01.T01:three_prime_UTR
TGCACAAGCATTATAGTACA+TGG 0.536128 5:+2784952 MsG0580024269.01.T01:intron
GGGAGGATCATTAGAAGTAG+AGG 0.538164 5:+2789000 None:intergenic
TTAATTAGCTATTGGCAGGA+AGG 0.541419 5:-2784876 MsG0580024270.01.T01:three_prime_UTR
ATTGCATGAACTTCTTCAGA+AGG 0.549621 5:-2787847 MsG0580024270.01.T01:CDS
ATTGCAGTTGTTCCGAAGAA+GGG 0.549801 5:-2785226 MsG0580024270.01.T01:intron
CTTGCACAAAATTATCAAGA+AGG 0.550602 5:+2787767 None:intergenic
TTCTAAGTTGTATATGGAGA+GGG 0.560215 5:-2784919 MsG0580024270.01.T01:three_prime_UTR
AGATAAACTAGATTCAAGAA+AGG 0.561327 5:-2785155 MsG0580024270.01.T01:three_prime_UTR
TTTCTTCATCTTCTCAAACA+AGG 0.563095 5:-2787735 MsG0580024270.01.T01:three_prime_UTR
TTTGGTCGTGCCATTTCACA+AGG 0.566627 5:-2788254 MsG0580024270.01.T01:intron
CTCCTAGGTGCAGATCTACA+TGG 0.569680 5:-2787152 MsG0580024270.01.T01:three_prime_UTR
ATCAAGGAAACGTGCAATGG+AGG 0.577785 5:-2789158 MsG0580024270.01.T01:CDS
TAATCTGTAGAGAAAGAAGA+TGG 0.577837 5:-2788105 MsG0580024270.01.T01:intron
AACCCATTTACTGGAACTGG+TGG 0.599675 5:-2786269 MsG0580024270.01.T01:three_prime_UTR
GGAGGATCATTAGAAGTAGA+GGG 0.608328 5:+2789001 None:intergenic
CTTCTAAGTTGTATATGGAG+AGG 0.612047 5:-2784920 MsG0580024270.01.T01:three_prime_UTR
TGATGGACGACATAAAGGCA+AGG 0.631995 5:+2788979 None:intergenic
AAGTTGTTGCTTAATCCACT+GGG 0.636456 5:-2787316 MsG0580024270.01.T01:intron
GCAGCACAAAAGTACATGCA+AGG 0.640542 5:-2787820 MsG0580024270.01.T01:CDS
GATGGACGACATAAAGGCAA+GGG 0.646981 5:+2788980 None:intergenic
TCTGCTCACAACTAATGGGG+AGG 0.647144 5:-2788043 MsG0580024270.01.T01:intron
GGACGACATAAAGGCAAGGG+AGG 0.652720 5:+2788983 None:intergenic
TCAAGCCAATGCATGACATG+TGG 0.654449 5:-2787351 MsG0580024270.01.T01:three_prime_UTR
TGAATCAAGGAAACGTGCAA+TGG 0.657298 5:-2789161 MsG0580024270.01.T01:CDS
AAAGGTTAGCAAATACATCA+CGG 0.664286 5:+2784805 MsG0580024269.01.T01:intron
GGATCATTAGAAGTAGAGGG+TGG 0.668208 5:+2789004 None:intergenic
ACCAGAAGAGGACAATAACA+AGG 0.669557 5:+2785012 MsG0580024269.01.T01:intron
AAATCTGCTCACAACTAATG+GGG 0.672764 5:-2788046 MsG0580024270.01.T01:CDS
TAGTTTATCTCCAAGCAGAG+TGG 0.678445 5:+2785168 MsG0580024269.01.T01:intron
TCATCCGATACACCCAACAA+AGG 0.686196 5:-2788959 MsG0580024270.01.T01:intron
CACCATGTAGATCTGCACCT+AGG 0.706017 5:+2787150 None:intergenic
GGTTAGCAAATACATCACGG+TGG 0.748854 5:+2784808 MsG0580024269.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTTTTTTACTTATTTA+AGG + Chr5:2785825-2785844 MsG0580024269.01.T01:intron 10.0%
!!! TTGATAATTAATGAATAATA+AGG - Chr5:2788428-2788447 MsG0580024270.01.T01:intron 10.0%
!!! TTTTTTATTTCATTTTCTTA+GGG - Chr5:2786183-2786202 MsG0580024270.01.T01:intron 10.0%
!! AAAAAGTTAATACATTTATG+TGG - Chr5:2785839-2785858 MsG0580024270.01.T01:intron 15.0%
!! TAAAAGAAAAGAAAAACAAA+GGG - Chr5:2785370-2785389 MsG0580024270.01.T01:intron 15.0%
!!! AATTAACAAATCAAAGTATT+TGG + Chr5:2789229-2789248 None:intergenic 15.0%
!!! ATTGAATCACTTTTTATTTA+GGG - Chr5:2788935-2788954 MsG0580024270.01.T01:intron 15.0%
!!! ATTTCATTTTCTTAGTAAAA+GGG + Chr5:2789019-2789038 None:intergenic 15.0%
!!! CTTTTTTATTTCATTTTCTT+AGG - Chr5:2786182-2786201 MsG0580024270.01.T01:intron 15.0%
!!! TATTTCATTTTCTTAGTAAA+AGG + Chr5:2789020-2789039 None:intergenic 15.0%
!!! TCTATTTTTGTTTTTTGTTT+TGG - Chr5:2787369-2787388 MsG0580024270.01.T01:intron 15.0%
!!! TTAATGATTTTCTTTCTTTT+AGG + Chr5:2785414-2785433 MsG0580024269.01.T01:intron 15.0%
!!! TTTGCTTATGATTATTTTTA+AGG - Chr5:2785879-2785898 MsG0580024270.01.T01:intron 15.0%
!!! TTTTCAATTTAATTAGCTAT+TGG - Chr5:2789588-2789607 MsG0580024270.01.T01:intron 15.0%
!! AAAAAAAAAATCCAAGTGTA+AGG + Chr5:2787807-2787826 None:intergenic 20.0%
!! AAATTTGAATTACCTTTGTT+GGG + Chr5:2785528-2785547 MsG0580024269.01.T01:intron 20.0%
!! AAGAATTAAGAAAGAGAAAA+TGG + Chr5:2788995-2789014 None:intergenic 20.0%
!! AATATTGTGTTTATGATGAT+TGG - Chr5:2784790-2784809 MsG0580024270.01.T01:three_prime_UTR 20.0%
!! AATTTGTAACTTCATTTATG+TGG - Chr5:2786323-2786342 MsG0580024270.01.T01:intron 20.0%
!! AGAATTAAGAAAGAGAAAAT+GGG + Chr5:2788994-2789013 None:intergenic 20.0%
!! AGTCATTCTTATAAACAATA+TGG - Chr5:2788715-2788734 MsG0580024270.01.T01:intron 20.0%
!! ATGAAACAACATAAAAAACA+TGG - Chr5:2786802-2786821 MsG0580024270.01.T01:intron 20.0%
!! ATTTCCATAAGCTAAAAAAT+AGG + Chr5:2785618-2785637 MsG0580024269.01.T01:intron 20.0%
!! ATTTGTAACTTCATTTATGT+GGG - Chr5:2786324-2786343 MsG0580024270.01.T01:intron 20.0%
!! CTAAAAGAAAAGAAAAACAA+AGG - Chr5:2785369-2785388 MsG0580024270.01.T01:intron 20.0%
!! TAAATTTGAATTACCTTTGT+TGG + Chr5:2785529-2785548 MsG0580024269.01.T01:intron 20.0%
!! TTAGAAAAACAAAATCGATT+TGG + Chr5:2785171-2785190 MsG0580024269.01.T01:intron 20.0%
!!! ATGTTTTTTATGTTGTTTCA+TGG + Chr5:2786803-2786822 None:intergenic 20.0%
!!! ATTCTATTTTAAGTAGTGAA+AGG + Chr5:2788295-2788314 None:intergenic 20.0%
!!! ATTTGCTACATATAATTGTA+CGG - Chr5:2788825-2788844 MsG0580024270.01.T01:intron 20.0%
!!! GATTGAATCACTTTTTATTT+AGG - Chr5:2788934-2788953 MsG0580024270.01.T01:intron 20.0%
!!! TTTCATTTTCTTAGTAAAAG+GGG + Chr5:2789018-2789037 None:intergenic 20.0%
!!! TTTTGATCTAACTAAAACTT+TGG + Chr5:2788970-2788989 None:intergenic 20.0%
! AAAACTTCAACTTAAGCAAA+CGG + Chr5:2789147-2789166 None:intergenic 25.0%
! AAAGCTCAAGATAAATGAAA+TGG + Chr5:2787430-2787449 None:intergenic 25.0%
! AACAGAATTAAACTCACTAT+AGG + Chr5:2785144-2785163 MsG0580024269.01.T01:intron 25.0%
! AAGAAGTTCATGCAATATTT+CGG + Chr5:2786621-2786640 None:intergenic 25.0%
! AAGTCAAATTAGAACAATCA+GGG + Chr5:2786559-2786578 None:intergenic 25.0%
! AAGTGATTCAATCGTAAATT+AGG + Chr5:2788927-2788946 None:intergenic 25.0%
! AATATTTCATATGCATCGAA+AGG - Chr5:2787527-2787546 MsG0580024270.01.T01:intron 25.0%
! ACAATTATATGTAGCAAATC+TGG + Chr5:2788824-2788843 None:intergenic 25.0%
! ACATAAAAAACATGGATCAA+TGG - Chr5:2786810-2786829 MsG0580024270.01.T01:intron 25.0%
! ACATTTACTGTATAGTGTAT+AGG - Chr5:2789625-2789644 MsG0580024270.01.T01:CDS 25.0%
! ACTATGCTAAAGTTTCAAAA+CGG + Chr5:2788465-2788484 None:intergenic 25.0%
! ACTTATTCAATATGACACTT+AGG - Chr5:2785578-2785597 MsG0580024270.01.T01:intron 25.0%
! AGACGAGTAAAAAAATCAAT+AGG + Chr5:2788892-2788911 None:intergenic 25.0%
! AGATAAACTAGATTCAAGAA+AGG - Chr5:2789317-2789336 MsG0580024270.01.T01:intron 25.0%
! ATGTGACACTTACAATATTA+TGG - Chr5:2789074-2789093 MsG0580024270.01.T01:CDS 25.0%
! CAAAAAACAAAAATAGAGTG+TGG + Chr5:2787366-2787385 None:intergenic 25.0%
! CATTAATACTTGAGAAATAC+AGG + Chr5:2788346-2788365 None:intergenic 25.0%
! CATTTACTGTATAGTGTATA+GGG - Chr5:2789626-2789645 MsG0580024270.01.T01:CDS 25.0%
! GCTTATATGTTTGAATGTAA+AGG - Chr5:2788592-2788611 MsG0580024270.01.T01:intron 25.0%
! TAAGTCAAATTAGAACAATC+AGG + Chr5:2786560-2786579 None:intergenic 25.0%
! TGAAATATAAAAACCAGAAG+AGG + Chr5:2789475-2789494 None:intergenic 25.0%
! TTACAATATTATGGCTGAAT+TGG - Chr5:2789083-2789102 MsG0580024270.01.T01:CDS 25.0%
!! AACTTATGTTGAATGCTTTT+AGG - Chr5:2787843-2787862 MsG0580024270.01.T01:CDS 25.0%
!! ATAAACCCAAATGCATTTTT+CGG - Chr5:2786081-2786100 MsG0580024270.01.T01:intron 25.0%
!! CTAACCTTTATTAGGTTTTT+GGG - Chr5:2789681-2789700 MsG0580024270.01.T01:CDS 25.0%
!! TAACCTTTATTAGGTTTTTG+GGG - Chr5:2789682-2789701 MsG0580024270.01.T01:CDS 25.0%
!! TGTATTTGCTAACCTTTATT+AGG - Chr5:2789673-2789692 MsG0580024270.01.T01:CDS 25.0%
!! TTCATTTATCTTGAGCTTTT+TGG - Chr5:2787431-2787450 MsG0580024270.01.T01:intron 25.0%
!! TTCATTTTCTTAGGGAATTT+CGG - Chr5:2786191-2786210 MsG0580024270.01.T01:intron 25.0%
!! TTTTAATGACTTATCAGTTC+TGG - Chr5:2789163-2789182 MsG0580024270.01.T01:CDS 25.0%
!! TTTTCATTTCGTCTTTCATT+GGG - Chr5:2786959-2786978 MsG0580024270.01.T01:intron 25.0%
!! TTTTTCATTTCGTCTTTCAT+TGG - Chr5:2786958-2786977 MsG0580024270.01.T01:intron 25.0%
!!! ATTGCCTATTTTTTAGCTTA+TGG - Chr5:2785611-2785630 MsG0580024270.01.T01:intron 25.0%
!!! CTTATGATTATTTTTAAGGC+TGG - Chr5:2785883-2785902 MsG0580024270.01.T01:intron 25.0%
!!! GATAATCCTTAAGTTTTAGA+TGG - Chr5:2789358-2789377 MsG0580024270.01.T01:intron 25.0%
!!! GATTGAACATATTGACTTTT+TGG + Chr5:2786488-2786507 None:intergenic 25.0%
!!! TTTCTTCATTTTGATTCTGA+AGG - Chr5:2786285-2786304 MsG0580024270.01.T01:three_prime_UTR 25.0%
AAAATCTGCTCACAACTAAT+GGG - Chr5:2786425-2786444 MsG0580024270.01.T01:intron 30.0%
AAACCCCAAAAACCTAATAA+AGG + Chr5:2789688-2789707 None:intergenic 30.0%
AAACTAGATTCAAGAAAGGT+TGG - Chr5:2789321-2789340 MsG0580024270.01.T01:intron 30.0%
AAAGGTTAGCAAATACATCA+CGG + Chr5:2789670-2789689 None:intergenic 30.0%
AAATTTGTCTGCATAACATG+TGG + Chr5:2788098-2788117 None:intergenic 30.0%
ACTTACTTATGGAACTCTTT+AGG + Chr5:2786841-2786860 None:intergenic 30.0%
AGAATTAAACTCACTATAGG+AGG + Chr5:2785141-2785160 MsG0580024269.01.T01:intron 30.0%
ATATGGCTGAGATTCTTATT+TGG - Chr5:2788732-2788751 MsG0580024270.01.T01:intron 30.0%
ATTACTTACACATGAATAGC+AGG + Chr5:2784862-2784881 MsG0580024269.01.T01:intron 30.0%
ATTATATGTAGCAAATCTGG+TGG + Chr5:2788821-2788840 None:intergenic 30.0%
ATTCAAATGAGAAACATTCC+CGG + Chr5:2787733-2787752 None:intergenic 30.0%
ATTTACAGTCACAGAAAGTT+GGG + Chr5:2786404-2786423 None:intergenic 30.0%
CAATTTAATTAGCTATTGGC+AGG - Chr5:2789592-2789611 MsG0580024270.01.T01:intron 30.0%
CTTGCACAAAATTATCAAGA+AGG + Chr5:2786708-2786727 None:intergenic 30.0%
GTAGTCTTCTAAGTTGTATA+TGG - Chr5:2789547-2789566 MsG0580024270.01.T01:intron 30.0%
TAAATACTTGCATGACTGTT+TGG + Chr5:2786052-2786071 MsG0580024269.01.T01:intron 30.0%
TAACTAGCAGACAACTATTT+AGG + Chr5:2788057-2788076 None:intergenic 30.0%
TAAGTAGTGAAAGGATTACT+CGG + Chr5:2788286-2788305 None:intergenic 30.0%
TAATCTGTAGAGAAAGAAGA+TGG - Chr5:2786367-2786386 MsG0580024270.01.T01:intron 30.0%
TACTATAATGCTTGTGCATT+TGG - Chr5:2789525-2789544 MsG0580024270.01.T01:intron 30.0%
TTATTCCTGGAACAATAAAC+AGG + Chr5:2787284-2787303 None:intergenic 30.0%
TTCTAAGTTGTATATGGAGA+GGG - Chr5:2789553-2789572 MsG0580024270.01.T01:intron 30.0%
TTCTTCATCTTCTCAAACAA+GGG - Chr5:2786738-2786757 MsG0580024270.01.T01:intron 30.0%
TTGTTCCAGGAATAATCAAT+TGG - Chr5:2787289-2787308 MsG0580024270.01.T01:intron 30.0%
TTTCATTTCGTCTTTCATTG+GGG - Chr5:2786960-2786979 MsG0580024270.01.T01:intron 30.0%
TTTCTTCATCTTCTCAAACA+AGG - Chr5:2786737-2786756 MsG0580024270.01.T01:intron 30.0%
! AAGATGCCATCTAAAACTTA+AGG + Chr5:2789367-2789386 None:intergenic 30.0%
! ACACTTTATGGAAGTATGTT+TGG - Chr5:2786877-2786896 MsG0580024270.01.T01:intron 30.0%
! ATGAACAGTGATTACTGATT+AGG + Chr5:2787080-2787099 None:intergenic 30.0%
! GAGAAGATGTATTTTATGAC+AGG - Chr5:2788638-2788657 MsG0580024270.01.T01:intron 30.0%
! GAGCTTTTTGGATAATTACA+AGG - Chr5:2787443-2787462 MsG0580024270.01.T01:intron 30.0%
! GCTAACCTTTATTAGGTTTT+TGG - Chr5:2789680-2789699 MsG0580024270.01.T01:CDS 30.0%
! TAAACCCAAATGCATTTTTC+GGG - Chr5:2786082-2786101 MsG0580024270.01.T01:intron 30.0%
! TTTACTTATTTAAGGCCTGA+TGG + Chr5:2785817-2785836 MsG0580024269.01.T01:intron 30.0%
! TTTCTAAGAACACCAAAAGT+AGG + Chr5:2787595-2787614 None:intergenic 30.0%
!! AAGGACAGTTATTTTGTCAT+TGG - Chr5:2787462-2787481 MsG0580024270.01.T01:intron 30.0%
!! ACAGTTATTTTGTCATTGGT+TGG - Chr5:2787466-2787485 MsG0580024270.01.T01:intron 30.0%
!! CTTCTTGATAATTTTGTGCA+AGG - Chr5:2786706-2786725 MsG0580024270.01.T01:intron 30.0%
!! GAAGTATTTTACGCAATCAT+TGG - Chr5:2787626-2787645 MsG0580024270.01.T01:intron 30.0%
!!! ATGATTATTTTTAAGGCTGG+AGG - Chr5:2785886-2785905 MsG0580024270.01.T01:intron 30.0%
!!! ATTGGTTTTGTCATCCAAAA+AGG - Chr5:2784808-2784827 MsG0580024270.01.T01:three_prime_UTR 30.0%
!!! CTACATGGTGCTATTATTTT+AGG - Chr5:2787335-2787354 MsG0580024270.01.T01:three_prime_UTR 30.0%
!!! GATTGGTGCTATGTTAATTT+TGG + Chr5:2784991-2785010 MsG0580024269.01.T01:intron 30.0%
!!! GTGTTCAGTTGCTTTTAAAA+TGG - Chr5:2788545-2788564 MsG0580024270.01.T01:intron 30.0%
!!! TGATTATTTTTAAGGCTGGA+GGG - Chr5:2785887-2785906 MsG0580024270.01.T01:intron 30.0%
!!! TTTTAGAGTTCAGACTTCAA+AGG + Chr5:2786512-2786531 None:intergenic 30.0%
AAATACAGGAATAACAGCTC+TGG + Chr5:2788332-2788351 None:intergenic 35.0%
AAATCTGCTCACAACTAATG+GGG - Chr5:2786426-2786445 MsG0580024270.01.T01:intron 35.0%
AACTGATGCCTGATAGAAAA+AGG + Chr5:2789405-2789424 None:intergenic 35.0%
ACAAACCTGTTTATTGTTCC+AGG - Chr5:2787276-2787295 MsG0580024270.01.T01:intron 35.0%
ACAAATACAGAACCCTTCTT+CGG + Chr5:2789261-2789280 None:intergenic 35.0%
AGAGACCTTGAACTTACTTA+TGG + Chr5:2786852-2786871 None:intergenic 35.0%
ATTACCTTTGTTGGGTGTAT+CGG + Chr5:2785520-2785539 MsG0580024269.01.T01:intron 35.0%
ATTCACAGGTTTCATTACAG+CGG + Chr5:2787571-2787590 None:intergenic 35.0%
ATTGCATGAACTTCTTCAGA+AGG - Chr5:2786625-2786644 MsG0580024270.01.T01:intron 35.0%
CAAAAATAAGCCAATCCAAG+CGG - Chr5:2785777-2785796 MsG0580024270.01.T01:intron 35.0%
CAAATTTGTACAGCAAACAC+TGG + Chr5:2788411-2788430 None:intergenic 35.0%
CATTTACAGTCACAGAAAGT+TGG + Chr5:2786405-2786424 None:intergenic 35.0%
CTTCTAAGTTGTATATGGAG+AGG - Chr5:2789552-2789571 MsG0580024270.01.T01:intron 35.0%
GAAAATCTGCTCACAACTAA+TGG - Chr5:2786424-2786443 MsG0580024270.01.T01:intron 35.0%
GAGTTCCATAAGTAAGTTCA+AGG - Chr5:2786844-2786863 MsG0580024270.01.T01:intron 35.0%
GATTTAATAACAGTACCCAG+TGG + Chr5:2787174-2787193 None:intergenic 35.0%
GGAATTCTTAGATTCATGCT+AGG - Chr5:2786981-2787000 MsG0580024270.01.T01:intron 35.0%
GGTCTCTAAACTACACTTTA+TGG - Chr5:2786865-2786884 MsG0580024270.01.T01:intron 35.0%
GTGTAAACTAACCCATTTAC+TGG - Chr5:2788194-2788213 MsG0580024270.01.T01:intron 35.0%
TAGTAAGATGAGCTTCTGAT+TGG - Chr5:2787681-2787700 MsG0580024270.01.T01:three_prime_UTR 35.0%
TGCACAAGCATTATAGTACA+TGG + Chr5:2789523-2789542 None:intergenic 35.0%
TGTGCAATTCATCAATAGCA+AGG + Chr5:2787196-2787215 None:intergenic 35.0%
TTAATTAGCTATTGGCAGGA+AGG - Chr5:2789596-2789615 MsG0580024270.01.T01:intron 35.0%
TTATCAATGCTGCTGAATCA+AGG - Chr5:2785298-2785317 MsG0580024270.01.T01:intron 35.0%
TTGAGCCAATTGATTATTCC+TGG + Chr5:2787297-2787316 None:intergenic 35.0%
! AAAGTTGTTGCTTAATCCAC+TGG - Chr5:2787155-2787174 MsG0580024270.01.T01:three_prime_UTR 35.0%
! AAGTTGTTGCTTAATCCACT+GGG - Chr5:2787156-2787175 MsG0580024270.01.T01:three_prime_UTR 35.0%
! AGTGTAAGGAGCATGAAAAA+AGG + Chr5:2787793-2787812 None:intergenic 35.0%
! GATGTATTTTATGACAGGCA+TGG - Chr5:2788643-2788662 MsG0580024270.01.T01:intron 35.0%
! GGTTTAGCATTTTCTGTTGT+AGG + Chr5:2787409-2787428 None:intergenic 35.0%
! GTGTAAGGAGCATGAAAAAA+GGG + Chr5:2787792-2787811 None:intergenic 35.0%
! TCAGCACTTTTATTCTCTCA+CGG - Chr5:2787236-2787255 MsG0580024270.01.T01:intron 35.0%
! TTAGGGAATTTCGGTCTTTT+TGG - Chr5:2786200-2786219 MsG0580024270.01.T01:intron 35.0%
! TTGACTCGAAAGTCGTTAAT+AGG - Chr5:2789046-2789065 MsG0580024270.01.T01:CDS 35.0%
! TTGGTGTTCTTAGAAAGAGT+TGG - Chr5:2787599-2787618 MsG0580024270.01.T01:intron 35.0%
! TTTTTCATGCTCCTTACACT+TGG - Chr5:2787793-2787812 MsG0580024270.01.T01:exon 35.0%
!! AGCAGCATTGATAAGAAAAC+TGG + Chr5:2785292-2785311 MsG0580024269.01.T01:intron 35.0%
!! AGCTGTTTCTACATTTCAAG+AGG - Chr5:2785938-2785957 MsG0580024270.01.T01:intron 35.0%
!! GCTGTTTCTACATTTCAAGA+GGG - Chr5:2785939-2785958 MsG0580024270.01.T01:intron 35.0%
!! TTGTACTACAAGTTGATTGC+TGG - Chr5:2789277-2789296 MsG0580024270.01.T01:intron 35.0%
AATTGCAGTTGTTCCGAAGA+AGG - Chr5:2789245-2789264 MsG0580024270.01.T01:intron 40.0%
ACACAGAATTGTGATGCTCA+AGG + Chr5:2789123-2789142 None:intergenic 40.0%
ACAGAAAGTTGGGCATATTC+TGG + Chr5:2786394-2786413 None:intergenic 40.0%
ACCAGAAGAGGACAATAACA+AGG + Chr5:2789463-2789482 None:intergenic 40.0%
ACGAGGGAAGAAGAGAAATA+AGG + Chr5:2784926-2784945 MsG0580024269.01.T01:intron 40.0%
ACTAACCCATTTACTGGAAC+TGG - Chr5:2788200-2788219 MsG0580024270.01.T01:intron 40.0%
AGAAAGCGAATAGGTTATCG+TGG + Chr5:2785055-2785074 MsG0580024269.01.T01:intron 40.0%
ATAAAGACATGAGCATCCGT+GGG + Chr5:2787502-2787521 None:intergenic 40.0%
ATACACTACCACAAACTGCA+GGG + Chr5:2786924-2786943 None:intergenic 40.0%
ATGTTGCTGGCAATAGAAAG+AGG + Chr5:2784951-2784970 MsG0580024269.01.T01:intron 40.0%
ATTGCAGTTGTTCCGAAGAA+GGG - Chr5:2789246-2789265 MsG0580024270.01.T01:intron 40.0%
CAAGAGGGAAATAAGCAATG+TGG - Chr5:2785954-2785973 MsG0580024270.01.T01:intron 40.0%
CAATGCATGTTGAGTCATCT+AGG + Chr5:2787962-2787981 None:intergenic 40.0%
CAGTACAGTACCTTGTGAAA+TGG + Chr5:2786231-2786250 MsG0580024269.01.T01:CDS 40.0%
CGAGGGAAGAAGAGAAATAA+GGG + Chr5:2784925-2784944 MsG0580024269.01.T01:intron 40.0%
CTAGCAGACAACTATTTAGG+AGG + Chr5:2788054-2788073 None:intergenic 40.0%
CTAGCCCGAAAAATGCATTT+GGG + Chr5:2786089-2786108 MsG0580024269.01.T01:intron 40.0%
CTATGGAGGAAGATGAAGAT+TGG + Chr5:2785008-2785027 MsG0580024269.01.T01:intron 40.0%
GACAACTATTTAGGAGGTAG+AGG + Chr5:2788048-2788067 None:intergenic 40.0%
GACTGTGTTAGAAAGCGAAT+AGG + Chr5:2785064-2785083 MsG0580024269.01.T01:intron 40.0%
GCAATAGAAAGAGGATACGA+GGG + Chr5:2784942-2784961 MsG0580024269.01.T01:intron 40.0%
GGAGGATCATTAGAAGTAGA+GGG + Chr5:2785474-2785493 MsG0580024269.01.T01:intron 40.0%
GGTTGGATGTTTATTTCCCA+CGG - Chr5:2787483-2787502 MsG0580024270.01.T01:intron 40.0%
TAGTTTATCTCCAAGCAGAG+TGG + Chr5:2789307-2789326 None:intergenic 40.0%
TATACACTACCACAAACTGC+AGG + Chr5:2786925-2786944 None:intergenic 40.0%
TATCCACATGTTATGCAGTG+TGG - Chr5:2788170-2788189 MsG0580024270.01.T01:intron 40.0%
TCATCTTCCTCCATAGAACA+CGG - Chr5:2785012-2785031 MsG0580024270.01.T01:three_prime_UTR 40.0%
TCTAGCCCGAAAAATGCATT+TGG + Chr5:2786090-2786109 MsG0580024269.01.T01:intron 40.0%
TCTTTCCACATGTCATGCAT+TGG + Chr5:2787129-2787148 None:intergenic 40.0%
TGAATCAAGGAAACGTGCAA+TGG - Chr5:2785311-2785330 MsG0580024270.01.T01:intron 40.0%
TGATACAGAAGAGATGTTGC+TGG + Chr5:2784964-2784983 MsG0580024269.01.T01:intron 40.0%
TGCTTCAAAACCGTGTTCTA+TGG + Chr5:2785025-2785044 MsG0580024269.01.T01:intron 40.0%
TTCAAAACCGTGTTCTATGG+AGG + Chr5:2785022-2785041 MsG0580024269.01.T01:intron 40.0%
! AACCTGTGAATGCCTACTTT+TGG - Chr5:2787580-2787599 MsG0580024270.01.T01:intron 40.0%
! ACATGAGGCCTTTTTCTATC+AGG - Chr5:2789394-2789413 MsG0580024270.01.T01:intron 40.0%
! ATGAGCTTCTGATTGGTTCA+AGG - Chr5:2787688-2787707 MsG0580024270.01.T01:three_prime_UTR 40.0%
! CTGCTTGTTACTCAAGAAAG+CGG + Chr5:2788688-2788707 None:intergenic 40.0%
! GTGAAACTGCAGAAGCTTTT+GGG - Chr5:2788237-2788256 MsG0580024270.01.T01:intron 40.0%
! GTTGAGTCATCTAGGAGTTT+TGG + Chr5:2787954-2787973 None:intergenic 40.0%
! TTTAAGGCCTGATGGAACAA+TGG + Chr5:2785809-2785828 MsG0580024269.01.T01:intron 40.0%
!! ACCTTGTTATTGTCCTCTTC+TGG - Chr5:2789459-2789478 MsG0580024270.01.T01:intron 40.0%
!! CAGTTTTCTTCTGAAAGAGG+AGG - Chr5:2786587-2786606 MsG0580024270.01.T01:intron 40.0%
!! CTGCAGTTTTCTTCTGAAAG+AGG - Chr5:2786584-2786603 MsG0580024270.01.T01:intron 40.0%
!!! ATAGAACACGGTTTTGAAGC+AGG - Chr5:2785024-2785043 MsG0580024270.01.T01:three_prime_UTR 40.0%
AACCCATTTACTGGAACTGG+TGG - Chr5:2788203-2788222 MsG0580024270.01.T01:intron 45.0%
ACTCCACACTGCATAACATG+TGG + Chr5:2788176-2788195 None:intergenic 45.0%
ATACCTAGGTCTTCTTCTCC+GGG - Chr5:2787712-2787731 MsG0580024270.01.T01:three_prime_UTR 45.0%
ATCAAGGAAACGTGCAATGG+AGG - Chr5:2785314-2785333 MsG0580024270.01.T01:intron 45.0%
ATGCCACCAGTTCCAGTAAA+TGG + Chr5:2788209-2788228 None:intergenic 45.0%
CACCAAAAGTAGGCATTCAC+AGG + Chr5:2787585-2787604 None:intergenic 45.0%
CATAAAGACATGAGCATCCG+TGG + Chr5:2787503-2787522 None:intergenic 45.0%
CATACCTAGGTCTTCTTCTC+CGG - Chr5:2787711-2787730 MsG0580024270.01.T01:three_prime_UTR 45.0%
GACCATACCATTGTTCCATC+AGG - Chr5:2785799-2785818 MsG0580024270.01.T01:intron 45.0%
GAGGGAAATAAGCAATGTGG+AGG - Chr5:2785957-2785976 MsG0580024270.01.T01:intron 45.0%
GATGGACGACATAAAGGCAA+GGG + Chr5:2785495-2785514 MsG0580024269.01.T01:intron 45.0%
GGATCATTAGAAGTAGAGGG+TGG + Chr5:2785471-2785490 MsG0580024269.01.T01:intron 45.0%
GGCAATAGAAAGAGGATACG+AGG + Chr5:2784943-2784962 MsG0580024269.01.T01:intron 45.0%
GGCATCTTTCGTTTCACATG+AGG - Chr5:2789379-2789398 MsG0580024270.01.T01:intron 45.0%
GGGAGGATCATTAGAAGTAG+AGG + Chr5:2785475-2785494 MsG0580024269.01.T01:intron 45.0%
GGGCTGTAGTAGTAATGACA+AGG + Chr5:2786904-2786923 None:intergenic 45.0%
GGTTAGCAAATACATCACGG+TGG + Chr5:2789667-2789686 None:intergenic 45.0%
TCAAGCCAATGCATGACATG+TGG - Chr5:2787121-2787140 MsG0580024270.01.T01:intron 45.0%
TCATCCGATACACCCAACAA+AGG - Chr5:2785513-2785532 MsG0580024270.01.T01:intron 45.0%
TCATTAGAAGTAGAGGGTGG+TGG + Chr5:2785468-2785487 MsG0580024269.01.T01:intron 45.0%
TGCCACCAGTTCCAGTAAAT+GGG + Chr5:2788208-2788227 None:intergenic 45.0%
TTGTTGGGTGTATCGGATGA+TGG + Chr5:2785513-2785532 MsG0580024269.01.T01:intron 45.0%
! CAATTGGCTCAATGTCTCCT+AGG - Chr5:2787305-2787324 MsG0580024270.01.T01:intron 45.0%
! CGTGAAACTGCAGAAGCTTT+TGG - Chr5:2788236-2788255 MsG0580024270.01.T01:intron 45.0%
! GATTGGTTCAAGGCATACCT+AGG - Chr5:2787698-2787717 MsG0580024270.01.T01:three_prime_UTR 45.0%
! GCAGCACAAAAGTACATGCA+AGG - Chr5:2786652-2786671 MsG0580024270.01.T01:intron 45.0%
! TGATGGACGACATAAAGGCA+AGG + Chr5:2785496-2785515 MsG0580024269.01.T01:intron 45.0%
!! TCGGATGATGGACGACATAA+AGG + Chr5:2785501-2785520 MsG0580024269.01.T01:intron 45.0%
!! TTTGGTCGTGCCATTTCACA+AGG - Chr5:2786218-2786237 MsG0580024270.01.T01:three_prime_UTR 45.0%
!! TTATAGTAAATAAAAAAAAA+AGG - Chr5:2784884-2784903 MsG0580024270.01.T01:three_prime_UTR 5.0%
ACGAACTCAGTTCACGATGG+CGG + Chr5:2785118-2785137 MsG0580024269.01.T01:intron 50.0%
AGGACGAACTCAGTTCACGA+TGG + Chr5:2785121-2785140 MsG0580024269.01.T01:intron 50.0%
ATGGTATGGTCCGCTTGGAT+TGG + Chr5:2785790-2785809 MsG0580024269.01.T01:intron 50.0%
ATTCCCGGAGAAGAAGACCT+AGG + Chr5:2787718-2787737 None:intergenic 50.0%
CACCATGTAGATCTGCACCT+AGG + Chr5:2787325-2787344 None:intergenic 50.0%
CCAAAAAGGTCTAGCGATGC+AGG - Chr5:2784822-2784841 MsG0580024270.01.T01:three_prime_UTR 50.0%
CTCCTAGGTGCAGATCTACA+TGG - Chr5:2787320-2787339 MsG0580024270.01.T01:three_prime_UTR 50.0%
GAAACGTGCAATGGAGGCTT+TGG - Chr5:2785320-2785339 MsG0580024270.01.T01:intron 50.0%
GAACAATGGTATGGTCCGCT+TGG + Chr5:2785795-2785814 MsG0580024269.01.T01:intron 50.0%
GGCCTGATGGAACAATGGTA+TGG + Chr5:2785804-2785823 MsG0580024269.01.T01:intron 50.0%
TACTACAGCCCTGCAGTTTG+TGG - Chr5:2786913-2786932 MsG0580024270.01.T01:intron 50.0%
TCTGCTCACAACTAATGGGG+AGG - Chr5:2786429-2786448 MsG0580024270.01.T01:intron 50.0%
! CCTGCATCGCTAGACCTTTT+TGG + Chr5:2784825-2784844 MsG0580024269.01.T01:intron 50.0%
! CGTGCCACTGGGAATGAAAA+GGG + Chr5:2787759-2787778 None:intergenic 50.0%
! TCCTCCCTTTTCATTCCCAG+TGG - Chr5:2787752-2787771 MsG0580024270.01.T01:three_prime_UTR 50.0%
CGAACTCAGTTCACGATGGC+GGG + Chr5:2785117-2785136 MsG0580024269.01.T01:intron 55.0%
GAACTCAGTTCACGATGGCG+GGG + Chr5:2785116-2785135 MsG0580024269.01.T01:intron 55.0%
GCCACTGGGAATGAAAAGGG+AGG + Chr5:2787756-2787775 None:intergenic 55.0%
GGACGACATAAAGGCAAGGG+AGG + Chr5:2785492-2785511 MsG0580024269.01.T01:intron 55.0%
TGCTGGAAAGCCACTCTGCT+TGG - Chr5:2789294-2789313 MsG0580024270.01.T01:intron 55.0%
! GCGTGCCACTGGGAATGAAA+AGG + Chr5:2787760-2787779 None:intergenic 55.0%
! GTATGCGTATGCGTGCCACT+GGG + Chr5:2787770-2787789 None:intergenic 55.0%
GGTATGCGTATGCGTGCCAC+TGG + Chr5:2787771-2787790 None:intergenic 60.0%
CACGAACCGTAGACGGAGGG+CGG + Chr5:2785093-2785112 MsG0580024269.01.T01:intron 65.0%
GGGCACGAACCGTAGACGGA+GGG + Chr5:2785096-2785115 MsG0580024269.01.T01:intron 65.0%
TCTGCTCCGCCCTCCGTCTA+CGG - Chr5:2785084-2785103 MsG0580024270.01.T01:three_prime_UTR 65.0%
GGCGGGGCACGAACCGTAGA+CGG + Chr5:2785100-2785119 MsG0580024269.01.T01:intron 70.0%
GGGGCACGAACCGTAGACGG+AGG + Chr5:2785097-2785116 MsG0580024269.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 2784787 2789707 2784787 ID=MsG0580024270.01;Name=MsG0580024270.01
Chr5 mRNA 2784787 2789707 2784787 ID=MsG0580024270.01.T01;Parent=MsG0580024270.01;Name=MsG0580024270.01.T01;_AED=0.21;_eAED=0.21;_QI=0|0.87|0.88|0.88|0.5|0.44|9|847|178
Chr5 exon 2784787 2785241 2784787 ID=MsG0580024270.01.T01:exon:948;Parent=MsG0580024270.01.T01
Chr5 exon 2786207 2786306 2786207 ID=MsG0580024270.01.T01:exon:947;Parent=MsG0580024270.01.T01
Chr5 exon 2787138 2787196 2787138 ID=MsG0580024270.01.T01:exon:946;Parent=MsG0580024270.01.T01
Chr5 exon 2787317 2787383 2787317 ID=MsG0580024270.01.T01:exon:945;Parent=MsG0580024270.01.T01
Chr5 exon 2787640 2787904 2787640 ID=MsG0580024270.01.T01:exon:944;Parent=MsG0580024270.01.T01
Chr5 exon 2788044 2788117 2788044 ID=MsG0580024270.01.T01:exon:943;Parent=MsG0580024270.01.T01
Chr5 exon 2788255 2788290 2788255 ID=MsG0580024270.01.T01:exon:942;Parent=MsG0580024270.01.T01
Chr5 exon 2788960 2789201 2788960 ID=MsG0580024270.01.T01:exon:941;Parent=MsG0580024270.01.T01
Chr5 exon 2789622 2789707 2789622 ID=MsG0580024270.01.T01:exon:940;Parent=MsG0580024270.01.T01
Chr5 CDS 2789622 2789707 2789622 ID=MsG0580024270.01.T01:cds;Parent=MsG0580024270.01.T01
Chr5 CDS 2788960 2789201 2788960 ID=MsG0580024270.01.T01:cds;Parent=MsG0580024270.01.T01
Chr5 CDS 2788255 2788290 2788255 ID=MsG0580024270.01.T01:cds;Parent=MsG0580024270.01.T01
Chr5 CDS 2788044 2788117 2788044 ID=MsG0580024270.01.T01:cds;Parent=MsG0580024270.01.T01
Chr5 CDS 2787806 2787904 2787806 ID=MsG0580024270.01.T01:cds;Parent=MsG0580024270.01.T01
Chr5 three_prime_UTR 2787640 2787805 2787640 ID=MsG0580024270.01.T01:three_prime_utr;Parent=MsG0580024270.01.T01
Chr5 three_prime_UTR 2787317 2787383 2787317 ID=MsG0580024270.01.T01:three_prime_utr;Parent=MsG0580024270.01.T01
Chr5 three_prime_UTR 2787138 2787196 2787138 ID=MsG0580024270.01.T01:three_prime_utr;Parent=MsG0580024270.01.T01
Chr5 three_prime_UTR 2786207 2786306 2786207 ID=MsG0580024270.01.T01:three_prime_utr;Parent=MsG0580024270.01.T01
Chr5 three_prime_UTR 2784787 2785241 2784787 ID=MsG0580024270.01.T01:three_prime_utr;Parent=MsG0580024270.01.T01
Gene Sequence

>MsG0580024270.01.T01

ATGAATATTGTGTTTATGATGATTGGTTTTGTCATCCAAAAAGGTCTAGCGATGCAGGTTGTTGCTCGCTTGCCTGCTATTCATGTTTTTCTTATCAATGCTGCTGAATCAAGGAAACGTGCAATGGAGGCTTTGGAGAGAAGAATTCAAACTGAACATAAACTAAAAGAAAAGAAAAACAAAGGGATAAAATCATCAACATATCCTAAAAGAAAGAAAATCATTAAGTCTATAAACGAAGATGTAAAATCATCTATTCCACCACCCTCTACTTCTAATGATCCTCCCTTGCCTTTATGTCGTCCATCATCCGATACACCCAACAAAGGGAATTTCGGTCTTTTTGGTCGTGCCATTTCACAAGAGAAAGAAGATGGTCCAGAATATGCCCAACTTTCTGTGACTGTAAATGAAAATCTGCTCACAACTAATGGGGAGTTTTCTTCTGAAAGAGGAGGTTCAGTTTCCGAAATATTGCATGAACTTCTTCAGAAGGAGATGCAGCACAAAAGTACATGCAAGGATCTAGAAGCATGA
Protein sequence

>MsG0580024270.01.T01

MNIVFMMIGFVIQKGLAMQVVARLPAIHVFLINAAESRKRAMEALERRIQTEHKLKEKKNKGIKSSTYPKRKKIIKSINEDVKSSIPPPSTSNDPPLPLCRPSSDTPNKGNFGLFGRAISQEKEDGPEYAQLSVTVNENLLTTNGEFSSERGGSVSEILHELLQKEMQHKSTCKDLEA*