AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042622.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042622.01.T01 MTR_8g018320 89.815 108 7 1 1 108 1 104 1.12e-65 193
MsG0880042622.01.T01 MTR_8g018360 89.720 107 7 1 1 107 1 103 3.13e-63 190
MsG0880042622.01.T01 MTR_2g099630 62.617 107 34 3 1 106 1 102 1.18e-37 123
MsG0880042622.01.T01 MTR_4g125990 52.542 118 43 3 2 107 3 119 1.37e-29 103
MsG0880042622.01.T01 MTR_3g104640 55.238 105 42 2 4 107 6 106 2.09e-20 79.3
MsG0880042622.01.T01 MTR_7g109290 63.043 46 17 0 62 107 42 87 4.04e-16 67.8
MsG0880042622.01.T01 MTR_1g087730 52.000 50 24 0 58 107 53 102 5.55e-15 65.5
MsG0880042622.01.T01 MTR_1g015810 40.741 108 51 3 11 108 15 119 5.34e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042622.01.T01 AT2G19460 44.737 114 53 4 2 107 3 114 1.33e-21 82.8
MsG0880042622.01.T01 AT5G11970 44.231 104 49 2 4 107 6 100 3.08e-21 81.3
MsG0880042622.01.T01 AT2G19460 44.737 114 53 4 2 107 55 166 3.82e-21 82.8
MsG0880042622.01.T01 AT3G13910 60.656 61 21 1 47 104 39 99 7.26e-17 70.1
MsG0880042622.01.T01 AT3G13910 57.812 64 24 1 47 107 39 102 8.09e-17 70.5
MsG0880042622.01.T01 AT1G72720 37.302 126 59 6 3 108 2 127 9.22e-13 60.5
MsG0880042622.01.T01 AT2G47480 43.333 60 34 0 48 107 47 106 2.23e-11 56.2
MsG0880042622.01.T01 AT3G05725 52.830 53 24 1 55 107 69 120 2.82e-11 56.2

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATCTTGCATGCTTGA+TGG 0.267870 8:+11555509 None:intergenic
ACTTTGATCCTTGCATGATT+TGG 0.271027 8:+11555568 None:intergenic
AGAACATATGCAGCGAATTA+TGG 0.308921 8:-11555280 MsG0880042622.01.T01:CDS
CAGAGAAAGAAAAGAGTTGC+TGG 0.405976 8:-11555368 MsG0880042622.01.T01:CDS
TTGCAGATTGAAAGCTATAG+TGG 0.472998 8:-11555545 MsG0880042622.01.T01:CDS
TATAAAGTATATGATGTTGA+AGG 0.487646 8:-11555344 MsG0880042622.01.T01:CDS
GATGTTGAAGGGAAAATGAA+AGG 0.506752 8:-11555332 MsG0880042622.01.T01:CDS
ATGTTGAAGGGAAAATGAAA+GGG 0.537096 8:-11555331 MsG0880042622.01.T01:CDS
AGTTATAGCACAAACTATGC+TGG 0.548662 8:-11555485 MsG0880042622.01.T01:CDS
ATAAAGTATATGATGTTGAA+GGG 0.582571 8:-11555343 MsG0880042622.01.T01:CDS
CAGATTGAAAGCTATAGTGG+TGG 0.602253 8:-11555542 MsG0880042622.01.T01:CDS
ATTCAGATCCAAATCATGCA+AGG 0.617513 8:-11555576 MsG0880042622.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGTATATGATGTTGAA+GGG - Chr8:11555515-11555534 MsG0880042622.01.T01:CDS 20.0%
!! TATAAAGTATATGATGTTGA+AGG - Chr8:11555514-11555533 MsG0880042622.01.T01:CDS 20.0%
!!! CTTTTAAGAAGAGTTTAAGA+TGG - Chr8:11555551-11555570 MsG0880042622.01.T01:CDS 25.0%
ATGTTGAAGGGAAAATGAAA+GGG - Chr8:11555527-11555546 MsG0880042622.01.T01:CDS 30.0%
! ATCAGCTTTTGATCAAAACA+AGG - Chr8:11555402-11555421 MsG0880042622.01.T01:CDS 30.0%
! TTAGCAAAGCATCAAAAAGT+TGG - Chr8:11555446-11555465 MsG0880042622.01.T01:CDS 30.0%
ACTTTGATCCTTGCATGATT+TGG + Chr8:11555293-11555312 None:intergenic 35.0%
AGAACATATGCAGCGAATTA+TGG - Chr8:11555578-11555597 MsG0880042622.01.T01:CDS 35.0%
AGTTATAGCACAAACTATGC+TGG - Chr8:11555373-11555392 MsG0880042622.01.T01:CDS 35.0%
ATTCAGATCCAAATCATGCA+AGG - Chr8:11555282-11555301 MsG0880042622.01.T01:CDS 35.0%
GATGTTGAAGGGAAAATGAA+AGG - Chr8:11555526-11555545 MsG0880042622.01.T01:CDS 35.0%
TTGCAGATTGAAAGCTATAG+TGG - Chr8:11555313-11555332 MsG0880042622.01.T01:CDS 35.0%
CAGAGAAAGAAAAGAGTTGC+TGG - Chr8:11555490-11555509 MsG0880042622.01.T01:CDS 40.0%
CAGATTGAAAGCTATAGTGG+TGG - Chr8:11555316-11555335 MsG0880042622.01.T01:CDS 40.0%
! CTTTTCTTTCTCTGCAACTC+TGG + Chr8:11555484-11555503 None:intergenic 40.0%
CTCAGATCTTGCATGCTTGA+TGG + Chr8:11555352-11555371 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 11555277 11555603 11555277 ID=MsG0880042622.01;Name=MsG0880042622.01
Chr8 mRNA 11555277 11555603 11555277 ID=MsG0880042622.01.T01;Parent=MsG0880042622.01;Name=MsG0880042622.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|108
Chr8 exon 11555277 11555603 11555277 ID=MsG0880042622.01.T01:exon:728;Parent=MsG0880042622.01.T01
Chr8 CDS 11555277 11555603 11555277 ID=MsG0880042622.01.T01:cds;Parent=MsG0880042622.01.T01
Gene Sequence

>MsG0880042622.01.T01

ATGGAATTCAGATCCAAATCATGCAAGGATCAAAGTTTGCAGATTGAAAGCTATAGTGGTGGAAGAGTAGCTCCATCAAGCATGCAAGATCTGAGAAGTTATAGCACAAACTATGCTGGTAATTCATCAGCTTTTGATCAAAACAAGGTAGAAAAAAGTAAAAGTAAATTTAGCAAAGCATCAAAAAGTTGGAGCTTCAATGATCCAGAGTTGCAGAGAAAGAAAAGAGTTGCTGGATATAAAGTATATGATGTTGAAGGGAAAATGAAAGGGTCTTTTAAGAAGAGTTTAAGATGGATCAAGAACATATGCAGCGAATTATGGTGA

Protein sequence

>MsG0880042622.01.T01

MEFRSKSCKDQSLQIESYSGGRVAPSSMQDLRSYSTNYAGNSSAFDQNKVEKSKSKFSKASKSWSFNDPELQRKKRVAGYKVYDVEGKMKGSFKKSLRWIKNICSELW*