AlfalfaGEDB | MsG0580025598.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025598.01

KEGG: -
GO: GO:0030246
PFAM: PF00139

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025598.01.T02	XP_003612970.2	94.595	148	8	0	1	148	137	284	8.08E-97	290
MsG0580025598.01.T01	XP_003612970.2	93.95	281	17	0	1	281	1	281	0	541

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025598.01.T01	A9YWS7	93.95	281	17	0	1	281	1	281	0	541
MsG0580025598.01.T02	A9YWS7	94.595	148	8	0	1	148	137	284	1.00E-99	290

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025598.01.T01	A0A2K3N7K8	74.737	285	60	3	1	281	1	277	5.06e-149	427
MsG0580025598.01.T02	A0A392MSP2	81.081	148	28	0	1	148	133	280	1.23e-82	254

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0180005059.01	MsG0580025598.01	PPI
MsG0180005060.01	MsG0580025598.01	PPI
MsG0380014744.01	MsG0580025598.01	PPI
MsG0180004091.01	MsG0580025598.01	PPI
MsG0580025598.01	MsG0380013593.01	PPI
MsG0880045216.01	MsG0580025598.01	PPI
MsG0180004091.01	MsG0580025598.01	PPI
MsG0380014275.01	MsG0580025598.01	PPI
MsG0180005080.01	MsG0580025598.01	PPI
MsG0580025598.01	MsG0880045214.01	PPI
MsG0580025598.01	MsG0180005080.01	PPI
MsG0380013594.01	MsG0580025598.01	PPI
MsG0380013593.01	MsG0580025598.01	PPI
MsG0880042846.01	MsG0580025598.01	PPI
MsG0380014748.01	MsG0580025598.01	PPI
MsG0880045217.01	MsG0580025598.01	PPI
MsG0180004091.01	MsG0580025598.01	PPI
MsG0380014272.01	MsG0580025598.01	PPI
MsG0180004091.01	MsG0580025598.01	PPI
MsG0180005082.01	MsG0580025598.01	PPI
MsG0580025591.01	MsG0580025598.01	PPI
MsG0180004091.01	MsG0580025598.01	PPI
MsG0580025598.01	MsG0180004091.01	PPI
MsG0380013595.01	MsG0580025598.01	PPI
MsG0580025592.01	MsG0580025598.01	PPI
MsG0880045214.01	MsG0580025598.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025598.01.T02	MTR_5g031160	94.595	148	8	0	1	148	137	284	9.78e-101	290
MsG0580025598.01.T02	MTR_5g031140	78.621	145	31	0	1	145	136	280	9.43e-79	234
MsG0580025598.01.T02	MTR_5g031120	77.241	145	33	0	1	145	129	273	6.44e-77	230
MsG0580025598.01.T02	MTR_5g031100	75.172	145	35	1	1	145	129	272	8.73e-73	219
MsG0580025598.01.T02	MTR_5g031090	51.701	147	69	2	1	145	123	269	4.51e-46	151
MsG0580025598.01.T02	MTR_5g031030	51.020	147	66	3	1	145	126	268	4.42e-43	143
MsG0580025598.01.T02	MTR_8g068040	50.000	152	68	3	1	148	111	258	7.67e-41	137
MsG0580025598.01.T02	MTR_8g067980	49.660	147	65	4	1	145	114	253	5.75e-39	132
MsG0580025598.01.T02	MTR_8g068030	47.436	156	70	3	1	148	111	262	6.96e-39	132
MsG0580025598.01.T02	MTR_8g068050	47.973	148	68	4	1	145	126	267	8.49e-37	133
MsG0580025598.01.T02	MTR_8g067990	46.939	147	67	4	2	145	115	253	1.22e-35	124
MsG0580025598.01.T02	MTR_8g067940	45.395	152	73	7	1	145	127	275	1.64e-29	108
MsG0580025598.01.T02	MTR_1g090973	42.254	142	67	7	8	148	130	257	1.01e-24	96.3
MsG0580025598.01.T02	MTR_8g067960	39.456	147	80	4	1	145	96	235	3.48e-23	91.7
MsG0580025598.01.T02	MTR_0684s0020	40.136	147	72	6	1	145	121	253	3.80e-22	89.4
MsG0580025598.01.T02	MTR_0163s0020	40.136	147	72	6	1	145	122	254	4.03e-22	89.4
MsG0580025598.01.T02	MTR_4g093080	37.500	152	79	4	1	145	111	253	5.87e-22	91.3
MsG0580025598.01.T02	MTR_3g463370	42.647	136	67	7	14	145	88	216	9.26e-22	87.8
MsG0580025598.01.T02	MTR_1g014240	34.416	154	84	4	1	145	124	269	2.16e-20	86.7
MsG0580025598.01.T02	MTR_8g067735	36.054	147	83	5	3	145	133	272	8.41e-20	85.1
MsG0580025598.01.T02	MTR_8g067720	37.931	145	82	4	3	145	113	251	9.87e-20	84.7
MsG0580025598.01.T02	MTR_1g090957	34.899	149	79	6	1	147	118	250	1.80e-19	82.0
MsG0580025598.01.T02	MTR_8g067690	37.241	145	85	3	3	145	148	288	2.68e-19	83.6
MsG0580025598.01.T02	MTR_4g093140	38.060	134	72	4	15	145	133	258	3.03e-19	83.6
MsG0580025598.01.T02	MTR_3g054170	40.152	132	66	5	1	132	116	234	5.42e-19	80.5
MsG0580025598.01.T02	MTR_3g047140	40.132	152	70	6	2	145	117	255	7.69e-19	80.9
MsG0580025598.01.T02	MTR_4g093110	38.462	117	65	3	30	145	148	258	1.04e-18	82.0
MsG0580025598.01.T02	MTR_8g067930	38.732	142	80	4	6	145	117	253	1.17e-18	81.6
MsG0580025598.01.T02	MTR_3g054200	38.129	139	72	6	8	145	123	248	1.79e-18	79.3
MsG0580025598.01.T02	MTR_1g090943	38.194	144	75	7	3	146	126	255	3.31e-18	79.0
MsG0580025598.01.T02	MTR_8g023510	38.849	139	73	7	9	145	125	253	4.19e-18	78.6
MsG0580025598.01.T02	MTR_1g090930	35.374	147	83	5	1	147	125	259	5.81e-18	78.6
MsG0580025598.01.T02	MTR_3g027280	34.228	149	80	6	1	147	130	262	8.24e-18	77.8
MsG0580025598.01.T02	MTR_4g093040	39.394	132	74	4	12	140	49	177	2.28e-17	78.2
MsG0580025598.01.T02	MTR_8g067630	37.681	138	77	5	1	135	116	247	2.34e-17	78.2
MsG0580025598.01.T02	MTR_4g093040	39.394	132	74	4	12	140	131	259	2.50e-17	77.8
MsG0580025598.01.T02	MTR_7g115740	35.915	142	81	3	8	145	119	254	1.12e-16	76.3
MsG0580025598.01.T02	MTR_4g023810	37.313	134	69	6	14	145	128	248	1.42e-16	74.3
MsG0580025598.01.T02	MTR_1g090937	30.612	147	88	5	1	147	123	255	1.44e-16	74.3
MsG0580025598.01.T02	MTR_4g093050	35.200	125	74	4	23	145	141	260	4.90e-15	71.2
MsG0580025598.01.T02	MTR_3g054180	38.806	134	67	6	1	132	120	240	1.47e-14	68.9
MsG0580025598.01.T02	MTR_7g116130	32.414	145	88	4	3	145	119	255	2.66e-14	69.3
MsG0580025598.01.T02	MTR_4g093070	33.103	145	80	6	8	145	126	260	6.18e-13	65.5
MsG0580025598.01.T02	MTR_1g090960	30.769	130	55	3	7	136	114	208	1.60e-11	60.5
MsG0580025598.01.T01	MTR_5g031160	93.950	281	17	0	1	281	1	281	0.0	541
MsG0580025598.01.T01	MTR_5g031140	73.759	282	71	3	1	281	1	280	1.17e-146	413
MsG0580025598.01.T01	MTR_5g031120	72.426	272	72	3	12	281	3	273	2.37e-135	384
MsG0580025598.01.T01	MTR_5g031100	71.691	272	73	4	12	281	3	272	3.03e-131	373
MsG0580025598.01.T01	MTR_5g031090	50.000	240	118	2	44	281	30	269	6.32e-80	243
MsG0580025598.01.T01	MTR_5g031030	50.000	240	114	3	44	281	33	268	7.55e-79	240
MsG0580025598.01.T01	MTR_8g068040	41.455	275	133	5	14	284	8	258	4.79e-62	197
MsG0580025598.01.T01	MTR_8g068030	40.143	279	135	5	14	284	8	262	9.70e-59	189
MsG0580025598.01.T01	MTR_8g068050	43.333	240	126	5	45	281	35	267	8.29e-58	196
MsG0580025598.01.T01	MTR_8g067940	41.877	277	137	9	13	281	15	275	2.71e-57	185
MsG0580025598.01.T01	MTR_8g067980	47.137	227	107	8	58	281	37	253	9.94e-57	183
MsG0580025598.01.T01	MTR_8g067990	43.333	240	119	9	48	281	25	253	1.92e-53	175
MsG0580025598.01.T01	MTR_8g023510	36.626	243	136	7	41	281	27	253	1.63e-41	144
MsG0580025598.01.T01	MTR_4g093080	34.818	247	138	5	42	281	23	253	3.35e-41	151
MsG0580025598.01.T01	MTR_0684s0020	36.364	242	134	7	42	281	30	253	6.28e-40	140
MsG0580025598.01.T01	MTR_0163s0020	36.364	242	134	7	42	281	31	254	6.37e-40	140
MsG0580025598.01.T01	MTR_8g067960	38.739	222	124	7	62	281	24	235	2.15e-38	135
MsG0580025598.01.T01	MTR_1g014240	33.200	250	143	6	43	281	33	269	1.25e-37	140
MsG0580025598.01.T01	MTR_3g054170	39.474	228	118	8	42	268	26	234	2.29e-37	133
MsG0580025598.01.T01	MTR_3g047140	36.727	275	146	9	9	281	7	255	2.31e-37	134
MsG0580025598.01.T01	MTR_1g090973	34.520	281	151	12	8	284	6	257	2.88e-36	130
MsG0580025598.01.T01	MTR_4g023810	34.979	243	136	8	41	281	26	248	4.68e-36	130
MsG0580025598.01.T01	MTR_4g093140	37.104	221	120	6	66	281	52	258	2.20e-35	135
MsG0580025598.01.T01	MTR_8g067720	34.310	239	146	5	45	281	22	251	6.84e-35	133
MsG0580025598.01.T01	MTR_3g054200	33.696	276	152	10	8	281	2	248	9.16e-35	126
MsG0580025598.01.T01	MTR_8g067690	33.054	239	150	4	45	281	58	288	3.42e-34	131
MsG0580025598.01.T01	MTR_8g067630	34.348	230	138	6	45	271	28	247	3.99e-34	130
MsG0580025598.01.T01	MTR_8g067735	32.927	246	147	8	42	281	39	272	7.03e-34	130
MsG0580025598.01.T01	MTR_4g093110	35.455	220	125	6	66	281	52	258	1.09e-33	129
MsG0580025598.01.T01	MTR_4g093040	37.778	225	127	8	58	276	42	259	2.60e-32	125
MsG0580025598.01.T01	MTR_3g054180	33.829	269	146	9	3	268	1	240	5.69e-32	119
MsG0580025598.01.T01	MTR_7g115740	35.417	240	138	8	48	281	26	254	7.62e-32	124
MsG0580025598.01.T01	MTR_1g090943	35.366	246	138	12	39	281	27	254	8.51e-32	119
MsG0580025598.01.T01	MTR_3g027280	32.404	287	157	14	4	283	6	262	9.27e-32	119
MsG0580025598.01.T01	MTR_8g067930	33.473	239	146	6	47	281	24	253	1.48e-30	120
MsG0580025598.01.T01	MTR_7g116130	33.476	233	137	8	55	281	35	255	3.39e-28	114
MsG0580025598.01.T01	MTR_4g093040	39.779	181	101	6	100	276	1	177	6.34e-28	113
MsG0580025598.01.T01	MTR_4g093070	32.889	225	130	6	64	281	50	260	6.85e-28	113
MsG0580025598.01.T01	MTR_3g463370	33.198	247	108	10	40	281	22	216	1.51e-26	104
MsG0580025598.01.T01	MTR_1g090957	33.065	248	125	14	46	283	34	250	2.13e-26	104
MsG0580025598.01.T01	MTR_1g090937	30.864	243	142	12	46	283	34	255	9.00e-26	103
MsG0580025598.01.T01	MTR_1g090960	30.712	267	119	8	8	272	6	208	1.32e-25	102
MsG0580025598.01.T01	MTR_1g090930	30.104	289	158	12	4	283	6	259	1.59e-24	100
MsG0580025598.01.T01	MTR_4g093050	34.081	223	132	9	66	281	46	260	3.15e-24	102
MsG0580025598.01.T01	MTR_8g068050	40.179	112	66	1	45	156	35	145	8.47e-22	95.1
MsG0580025598.01.T01	MTR_5g025020	31.250	208	127	6	86	283	82	283	2.83e-18	85.1
MsG0580025598.01.T01	MTR_7g115740	36.232	138	81	5	48	183	26	158	7.62e-18	83.6
MsG0580025598.01.T01	MTR_7g062940	27.559	254	155	10	45	284	18	256	4.18e-15	75.5
MsG0580025598.01.T01	MTR_1g048360	29.646	226	115	11	71	281	60	256	5.89e-15	75.1
MsG0580025598.01.T01	MTR_1g099260	28.390	236	146	10	58	284	27	248	2.83e-13	70.1
MsG0580025598.01.T01	MTR_7g063030	27.556	225	136	8	73	284	45	255	6.54e-12	66.2
MsG0580025598.01.T01	MTR_7g063030	27.556	225	136	8	73	284	51	261	6.63e-12	66.2
MsG0580025598.01.T01	MTR_2g090410	29.487	234	140	10	61	281	738	959	2.08e-11	64.7
MsG0580025598.01.T01	MTR_3g068025	29.119	261	150	9	44	284	23	268	3.48e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025598.01.T02	AT5G10530	39.597	149	81	6	1	145	105	248	7.88e-22	90.9
MsG0580025598.01.T02	AT5G65600	36.552	145	83	5	1	140	122	262	1.22e-16	75.9
MsG0580025598.01.T01	AT5G10530	35.772	246	141	9	42	281	14	248	1.62e-34	132
MsG0580025598.01.T01	AT5G65600	34.034	238	141	8	46	276	34	262	1.07e-29	118
MsG0580025598.01.T01	AT5G55830	33.992	253	139	12	45	284	28	265	2.73e-19	88.2
MsG0580025598.01.T01	AT4G29050	28.736	261	153	10	39	284	17	259	8.05e-17	80.9
MsG0580025598.01.T01	AT4G29050	28.736	261	153	10	39	284	31	273	8.58e-17	80.9
MsG0580025598.01.T01	AT3G53810	27.308	260	158	10	41	284	18	262	3.04e-16	79.0
MsG0580025598.01.T01	AT2G37710	28.085	235	142	11	61	281	38	259	6.32e-15	75.1
MsG0580025598.01.T01	AT4G02420	28.322	286	154	11	15	281	5	258	2.20e-14	73.6
MsG0580025598.01.T01	AT5G06740	26.639	244	163	9	44	281	33	266	2.57e-14	73.2
MsG0580025598.01.T01	AT5G06740	26.639	244	163	9	44	281	21	254	2.88e-14	73.2
MsG0580025598.01.T01	AT4G02410	27.600	250	156	11	45	281	24	261	5.19e-14	72.4
MsG0580025598.01.T01	AT1G70110	29.204	226	132	8	73	284	46	257	3.05e-13	70.1
MsG0580025598.01.T01	AT5G03140	28.636	220	140	9	66	281	51	257	1.28e-11	65.5
MsG0580025598.01.T01	AT3G55550	29.268	246	145	8	50	281	26	256	1.81e-11	64.7
MsG0580025598.01.T01	AT4G04960	29.600	250	145	10	47	281	20	253	2.28e-11	64.3
MsG0580025598.01.T01	AT4G04960	29.200	250	146	9	47	281	20	253	3.54e-11	63.9
MsG0580025598.01.T01	AT3G16530	26.275	255	162	9	47	281	19	267	5.82e-11	62.0
MsG0580025598.01.T01	AT2G29220	30.942	223	126	10	73	281	51	259	9.37e-11	62.8

Find 59 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTCACACCAATTCATATT+TGG	0.103065	5:+21064176	MsG0580025598.01.T01:CDS
AAACACATGATATCATTAAT+TGG	0.208602	5:+21064710	MsG0580025598.01.T01:CDS
TCAAACTCAATAGCTAATAT+TGG	0.239967	5:-21064391	None:intergenic
TTTCTTGGCACAAAATTAAT+AGG	0.257648	5:-21064511	None:intergenic
AGACGGATTCGCTTTCTATA+TGG	0.282424	5:+21064279	MsG0580025598.01.T01:CDS
TTTCAGTTGTTGTTGATCTA+AGG	0.293081	5:+21064629	MsG0580025598.01.T01:CDS
GCTTCTCCTAAAGCATTTCT+TGG	0.304714	5:-21064526	None:intergenic
CTCTGCAACTACAGGAGAAT+TGG	0.320068	5:+21064684	MsG0580025598.01.T01:CDS
ATATTAGCTATTGAGTTTGA+TGG	0.346554	5:+21064394	MsG0580025598.01.T01:CDS
AGTGTGTTTGTGGCTTATCC+TGG	0.346816	5:+21064583	MsG0580025598.01.T01:CDS
AACACATGATATCATTAATT+GGG	0.351634	5:+21064711	MsG0580025598.01.T01:CDS
GTCACACGTCCAATGGTATT+TGG	0.354022	5:-21064151	None:intergenic
CAAACTCAATAGCTAATATT+GGG	0.357502	5:-21064390	None:intergenic
CCTCACACCAATTCATATTT+GGG	0.358786	5:+21064177	MsG0580025598.01.T01:CDS
TAAAAGATTAAGTGTGTTTG+TGG	0.366091	5:+21064573	MsG0580025598.01.T01:CDS
AAACCTATTCTAACCCATTC+AGG	0.369741	5:-21064661	None:intergenic
AATTCAAGCGGAGGTTATCT+TGG	0.376674	5:+21064328	MsG0580025598.01.T01:CDS
ACCGTTTGTGTTCACGATAA+AGG	0.395761	5:-21064242	None:intergenic
CTCACATTGGAATTGATGTT+GGG	0.397731	5:+21064458	MsG0580025598.01.T01:CDS
GACGGATTCGCTTTCTATAT+GGG	0.402319	5:+21064280	MsG0580025598.01.T01:CDS
TTGGTAGTGGTTTACCAGAT+TGG	0.415002	5:-21064132	None:intergenic
TTTGTGCCAAGAAATGCTTT+AGG	0.419423	5:+21064520	MsG0580025598.01.T01:CDS
TTGCCTGAATGGGTTAGAAT+AGG	0.427346	5:+21064658	MsG0580025598.01.T01:CDS
CGTCCAATGGTATTTGGTAG+TGG	0.452642	5:-21064145	None:intergenic
TCCTTTATCGTGAACACAAA+CGG	0.457315	5:+21064241	MsG0580025598.01.T01:CDS
ATTTGACATTGTGGTATTGT+AGG	0.461920	5:-21063964	None:intergenic
CCTCACATTGGAATTGATGT+TGG	0.465626	5:+21064457	MsG0580025598.01.T01:CDS
ATAGGTTTCTCTGCAACTAC+AGG	0.476749	5:+21064676	MsG0580025598.01.T01:CDS
TAGGCCACTGTGCATATTCA+AGG	0.478728	5:-21064492	None:intergenic
GTGTGTTTGTGGCTTATCCT+GGG	0.480055	5:+21064584	MsG0580025598.01.T01:CDS
CATTGTGGTATTGTAGGAAG+AGG	0.491943	5:-21063958	None:intergenic
AGGGGTGACACCTCAATCTC+CGG	0.500129	5:+21064082	MsG0580025598.01.T01:CDS
GTGGAATTCCACTGAGTCCC+AGG	0.500597	5:-21064601	None:intergenic
TAGAATGCCGCCGGAGATTG+AGG	0.514884	5:-21064092	None:intergenic
TCATTTCAATCTCCTCACAT+TGG	0.532234	5:+21064445	MsG0580025598.01.T01:CDS
CATATTTGGGATAAAACTAG+TGG	0.541599	5:+21064190	MsG0580025598.01.T01:CDS
AAAGCAATGGTTTCTGATTG+TGG	0.552586	5:-21064016	None:intergenic
AGAAAGCGAATCCGTCTCCA+TGG	0.555718	5:-21064273	None:intergenic
TAGTGGTTTACCAGATTGGT+CGG	0.559523	5:-21064128	None:intergenic
TGGCTTATCCTGGGACTCAG+TGG	0.570682	5:+21064593	MsG0580025598.01.T01:CDS
AAACCACTACCAAATACCAT+TGG	0.571050	5:+21064142	MsG0580025598.01.T01:CDS
AACAACAACTGAAACGCTAG+TGG	0.571657	5:-21064620	None:intergenic
TGTTAGTCTTAGAATGCCGC+CGG	0.574784	5:-21064101	None:intergenic
AGGCCACTGTGCATATTCAA+GGG	0.580431	5:-21064491	None:intergenic
ATTGTGGTATTGTAGGAAGA+GGG	0.581412	5:-21063957	None:intergenic
GGAGATTGAAATGATGAAGG+AGG	0.586223	5:-21064436	None:intergenic
TGAGGAGATTGAAATGATGA+AGG	0.593630	5:-21064439	None:intergenic
GAGGTATGTCACACGTCCAA+TGG	0.596733	5:-21064158	None:intergenic
CACGATAAAGGAGAAACTAG+TGG	0.616598	5:-21064230	None:intergenic
TTTGGTACTTCAAAATGTAG+CGG	0.622878	5:-21064304	None:intergenic
CCCAAATATGAATTGGTGTG+AGG	0.623292	5:-21064177	None:intergenic
ACACATGATATCATTAATTG+GGG	0.626231	5:+21064712	MsG0580025598.01.T01:CDS
GGTTCAGATCTCCATGGAGA+CGG	0.630900	5:+21064262	MsG0580025598.01.T01:CDS
ATGATTGTGATTTGACATTG+TGG	0.636628	5:-21063973	None:intergenic
GGTGACACCTCAATCTCCGG+CGG	0.674666	5:+21064085	MsG0580025598.01.T01:CDS
CCAACATCAATTCCAATGTG+AGG	0.681265	5:-21064457	None:intergenic
ACAAACGGTTCAGATCTCCA+TGG	0.693828	5:+21064256	MsG0580025598.01.T01:CDS
ATGTCAAATCACAATCATCG+TGG	0.734263	5:+21063978	MsG0580025598.01.T01:CDS
TTTCCCTTGAATATGCACAG+TGG	0.741060	5:+21064488	MsG0580025598.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAACACATGATATCATTAAT+TGG	+	Chr5:21064710-21064729	MsG0580025598.01.T01:CDS	20.0%
!!	AACACATGATATCATTAATT+GGG	+	Chr5:21064711-21064730	MsG0580025598.01.T01:CDS	20.0%
!!!	AATTCTGTTAGATTTTAAGT+TGG	-	Chr5:21063903-21063922	None:intergenic	20.0%
!	ACACATGATATCATTAATTG+GGG	+	Chr5:21064712-21064731	MsG0580025598.01.T01:CDS	25.0%
!	ATATTAGCTATTGAGTTTGA+TGG	+	Chr5:21064394-21064413	MsG0580025598.01.T01:CDS	25.0%
!	CAAACTCAATAGCTAATATT+GGG	-	Chr5:21064393-21064412	None:intergenic	25.0%
!	TAAAAGATTAAGTGTGTTTG+TGG	+	Chr5:21064573-21064592	MsG0580025598.01.T01:CDS	25.0%
!	TCAAACTCAATAGCTAATAT+TGG	-	Chr5:21064394-21064413	None:intergenic	25.0%
!	TTTCTTGGCACAAAATTAAT+AGG	-	Chr5:21064514-21064533	None:intergenic	25.0%
!!	AAAATCTCGACATATTTTTC+AGG	+	Chr5:21064062-21064081	MsG0580025598.01.T01:CDS	25.0%
!!	AAATCTCGACATATTTTTCA+GGG	+	Chr5:21064063-21064082	MsG0580025598.01.T01:CDS	25.0%
!!	AATAGCTAATATTGGGTTTT+TGG	-	Chr5:21064386-21064405	None:intergenic	25.0%
!!	GTTTTATCCCAAATATGAAT+TGG	-	Chr5:21064187-21064206	None:intergenic	25.0%
	ATGATTGTGATTTGACATTG+TGG	-	Chr5:21063976-21063995	None:intergenic	30.0%
	ATTTGACATTGTGGTATTGT+AGG	-	Chr5:21063967-21063986	None:intergenic	30.0%
	TTTGGTACTTCAAAATGTAG+CGG	-	Chr5:21064307-21064326	None:intergenic	30.0%
!	CATATTTGGGATAAAACTAG+TGG	+	Chr5:21064190-21064209	MsG0580025598.01.T01:CDS	30.0%
!	GAAGTACCAAAAAATTCAAG+CGG	+	Chr5:21064316-21064335	MsG0580025598.01.T01:CDS	30.0%
!	TTTCAGTTGTTGTTGATCTA+AGG	+	Chr5:21064629-21064648	MsG0580025598.01.T01:CDS	30.0%
!!	AATCTCGACATATTTTTCAG+GGG	+	Chr5:21064064-21064083	MsG0580025598.01.T01:CDS	30.0%
!!	TTTTGTTATGCTGAAAGCAA+TGG	-	Chr5:21064032-21064051	None:intergenic	30.0%
	AAACCACTACCAAATACCAT+TGG	+	Chr5:21064142-21064161	MsG0580025598.01.T01:CDS	35.0%
	AAACCTATTCTAACCCATTC+AGG	-	Chr5:21064664-21064683	None:intergenic	35.0%
	AAAGCAATGGTTTCTGATTG+TGG	-	Chr5:21064019-21064038	None:intergenic	35.0%
	ACCTCACACCAATTCATATT+TGG	+	Chr5:21064176-21064195	MsG0580025598.01.T01:CDS	35.0%
	ATGTCAAATCACAATCATCG+TGG	+	Chr5:21063978-21063997	MsG0580025598.01.T01:CDS	35.0%
	ATTGTGGTATTGTAGGAAGA+GGG	-	Chr5:21063960-21063979	None:intergenic	35.0%
	CCTCACACCAATTCATATTT+GGG	+	Chr5:21064177-21064196	MsG0580025598.01.T01:CDS	35.0%
	CTCACATTGGAATTGATGTT+GGG	+	Chr5:21064458-21064477	MsG0580025598.01.T01:CDS	35.0%
	TCATTTCAATCTCCTCACAT+TGG	+	Chr5:21064445-21064464	MsG0580025598.01.T01:CDS	35.0%
	TCCTTTATCGTGAACACAAA+CGG	+	Chr5:21064241-21064260	MsG0580025598.01.T01:CDS	35.0%
	TGAGGAGATTGAAATGATGA+AGG	-	Chr5:21064442-21064461	None:intergenic	35.0%
	TTTGTGCCAAGAAATGCTTT+AGG	+	Chr5:21064520-21064539	MsG0580025598.01.T01:CDS	35.0%
!!	AATATTGGGTTTTTGGATGG+AGG	-	Chr5:21064379-21064398	None:intergenic	35.0%
!!	GCTAATATTGGGTTTTTGGA+TGG	-	Chr5:21064382-21064401	None:intergenic	35.0%
!!	TGATGGTTTTACAAACGAGT+GGG	+	Chr5:21064411-21064430	MsG0580025598.01.T01:CDS	35.0%
!!	TTGATGGTTTTACAAACGAG+TGG	+	Chr5:21064410-21064429	MsG0580025598.01.T01:CDS	35.0%
!!!	TAACCTCCGCTTGAATTTTT+TGG	-	Chr5:21064325-21064344	None:intergenic	35.0%
	AACAACAACTGAAACGCTAG+TGG	-	Chr5:21064623-21064642	None:intergenic	40.0%
	AATTCAAGCGGAGGTTATCT+TGG	+	Chr5:21064328-21064347	MsG0580025598.01.T01:CDS	40.0%
	ACCGTTTGTGTTCACGATAA+AGG	-	Chr5:21064245-21064264	None:intergenic	40.0%
	ATAGGTTTCTCTGCAACTAC+AGG	+	Chr5:21064676-21064695	MsG0580025598.01.T01:CDS	40.0%
	CACGATAAAGGAGAAACTAG+TGG	-	Chr5:21064233-21064252	None:intergenic	40.0%
	CATTGTGGTATTGTAGGAAG+AGG	-	Chr5:21063961-21063980	None:intergenic	40.0%
	CCAACATCAATTCCAATGTG+AGG	-	Chr5:21064460-21064479	None:intergenic	40.0%
	CCCAAATATGAATTGGTGTG+AGG	-	Chr5:21064180-21064199	None:intergenic	40.0%
	CCTCACATTGGAATTGATGT+TGG	+	Chr5:21064457-21064476	MsG0580025598.01.T01:CDS	40.0%
	CTAACAAAAACCGACCAATC+TGG	+	Chr5:21064118-21064137	MsG0580025598.01.T01:CDS	40.0%
	GGAGATTGAAATGATGAAGG+AGG	-	Chr5:21064439-21064458	None:intergenic	40.0%
	GTACCAAAAAATTCAAGCGG+AGG	+	Chr5:21064319-21064338	MsG0580025598.01.T01:CDS	40.0%
	TAGTGGTTTACCAGATTGGT+CGG	-	Chr5:21064131-21064150	None:intergenic	40.0%
	TTTCCCTTGAATATGCACAG+TGG	+	Chr5:21064488-21064507	MsG0580025598.01.T01:CDS	40.0%
!	AGACGGATTCGCTTTCTATA+TGG	+	Chr5:21064279-21064298	MsG0580025598.01.T01:CDS	40.0%
!	ATGGAGGAAATGCATTTTCC+GGG	-	Chr5:21064363-21064382	None:intergenic	40.0%
!	GACGGATTCGCTTTCTATAT+GGG	+	Chr5:21064280-21064299	MsG0580025598.01.T01:CDS	40.0%
!	GATGGAGGAAATGCATTTTC+CGG	-	Chr5:21064364-21064383	None:intergenic	40.0%
!	GCTTCTCCTAAAGCATTTCT+TGG	-	Chr5:21064529-21064548	None:intergenic	40.0%
!!	TTATCTTGGTCTTTTCGACC+CGG	+	Chr5:21064342-21064361	MsG0580025598.01.T01:CDS	40.0%
!!	TTGCCTGAATGGGTTAGAAT+AGG	+	Chr5:21064658-21064677	MsG0580025598.01.T01:CDS	40.0%
!!	TTGGTAGTGGTTTACCAGAT+TGG	-	Chr5:21064135-21064154	None:intergenic	40.0%
!!!	AAGGAGTGTTTTGCCTGAAT+GGG	+	Chr5:21064648-21064667	MsG0580025598.01.T01:CDS	40.0%
!!!	TAAGGAGTGTTTTGCCTGAA+TGG	+	Chr5:21064647-21064666	MsG0580025598.01.T01:CDS	40.0%
	ACAAACGGTTCAGATCTCCA+TGG	+	Chr5:21064256-21064275	MsG0580025598.01.T01:CDS	45.0%
	AGGCCACTGTGCATATTCAA+GGG	-	Chr5:21064494-21064513	None:intergenic	45.0%
	AGTGTGTTTGTGGCTTATCC+TGG	+	Chr5:21064583-21064602	MsG0580025598.01.T01:CDS	45.0%
	CGTCCAATGGTATTTGGTAG+TGG	-	Chr5:21064148-21064167	None:intergenic	45.0%
	CTCTGCAACTACAGGAGAAT+TGG	+	Chr5:21064684-21064703	MsG0580025598.01.T01:CDS	45.0%
	GTCACACGTCCAATGGTATT+TGG	-	Chr5:21064154-21064173	None:intergenic	45.0%
	GTGTGTTTGTGGCTTATCCT+GGG	+	Chr5:21064584-21064603	MsG0580025598.01.T01:CDS	45.0%
	TAGGCCACTGTGCATATTCA+AGG	-	Chr5:21064495-21064514	None:intergenic	45.0%
!!	TGTTAGTCTTAGAATGCCGC+CGG	-	Chr5:21064104-21064123	None:intergenic	45.0%
	AGAAAGCGAATCCGTCTCCA+TGG	-	Chr5:21064276-21064295	None:intergenic	50.0%
	GAGGTATGTCACACGTCCAA+TGG	-	Chr5:21064161-21064180	None:intergenic	50.0%
	GGTTCAGATCTCCATGGAGA+CGG	+	Chr5:21064262-21064281	MsG0580025598.01.T01:CDS	50.0%
	AGGGGTGACACCTCAATCTC+CGG	+	Chr5:21064082-21064101	MsG0580025598.01.T01:CDS	55.0%
	GTGGAATTCCACTGAGTCCC+AGG	-	Chr5:21064604-21064623	None:intergenic	55.0%
	TAGAATGCCGCCGGAGATTG+AGG	-	Chr5:21064095-21064114	None:intergenic	55.0%
	TGGCTTATCCTGGGACTCAG+TGG	+	Chr5:21064593-21064612	MsG0580025598.01.T01:CDS	55.0%
	GGTGACACCTCAATCTCCGG+CGG	+	Chr5:21064085-21064104	MsG0580025598.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	21063890	21064744	21063890	ID=MsG0580025598.01;Name=MsG0580025598.01
Chr5	mRNA	21063890	21064744	21063890	ID=MsG0580025598.01.T01;Parent=MsG0580025598.01;Name=MsG0580025598.01.T01;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|284
Chr5	exon	21063890	21064744	21063890	ID=MsG0580025598.01.T01:exon:23521;Parent=MsG0580025598.01.T01
Chr5	CDS	21063890	21064744	21063890	ID=MsG0580025598.01.T01:cds;Parent=MsG0580025598.01.T01
Chr5	mRNA	21064298	21064744	21064298	ID=MsG0580025598.01.T02;Parent=MsG0580025598.01;Name=MsG0580025598.01.T02;_AED=0.24;_eAED=0.24;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|148
Chr5	exon	21064298	21064744	21064298	ID=MsG0580025598.01.T02:exon:23522;Parent=MsG0580025598.01.T02
Chr5	CDS	21064298	21064744	21064298	ID=MsG0580025598.01.T02:cds;Parent=MsG0580025598.01.T02
Chr5	mRNA	21064413	21064541	21064413	ID=MsG0580025598.01.T03;Parent=MsG0580025598.01;Name=MsG0580025598.01.T03;_AED=0.43;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|42
Chr5	exon	21064413	21064541	21064413	ID=MsG0580025598.01.T03:exon:23523;Parent=MsG0580025598.01.T03
Chr5	CDS	21064413	21064541	21064413	ID=MsG0580025598.01.T03:cds;Parent=MsG0580025598.01.T03
Chr5	mRNA	21064473	21064541	21064473	ID=MsG0580025598.01.T04;Parent=MsG0580025598.01;Name=MsG0580025598.01.T04;_AED=0.46;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|22
Chr5	exon	21064473	21064541	21064473	ID=MsG0580025598.01.T04:exon:23524;Parent=MsG0580025598.01.T04
Chr5	CDS	21064473	21064541	21064473	ID=MsG0580025598.01.T04:cds;Parent=MsG0580025598.01.T04

Gene Sequence

>MsG0580025598.01.T03

ATGGTTTTACAAACGAGTGGGATCCTCCTTCATCATTTCAATCTCCTCACATTGGAATTGATGTTGGGTCTATTGTTTCCCTTGAATATGCACAGTGGCCTATTAATTTTGTGCCAAGAAATGCTTTAG

>MsG0580025598.01.T02

ATGGGACCGCTACATTTTGAAGTACCAAAAAATTCAAGCGGAGGTTATCTTGGTCTTTTCGACCCGGAAAATGCATTTCCTCCATCCAAAAACCCAATATTAGCTATTGAGTTTGATGGTTTTACAAACGAGTGGGATCCTCCTTCATCATTTCAATCTCCTCACATTGGAATTGATGTTGGGTCTATTGTTTCCCTTGAATATGCACAGTGGCCTATTAATTTTGTGCCAAGAAATGCTTTAGGAGAAGCTAGTATAAACTATAACTCTGAATCTAAAAGATTAAGTGTGTTTGTGGCTTATCCTGGGACTCAGTGGAATTCCACTAGCGTTTCAGTTGTTGTTGATCTAAGGAGTGTTTTGCCTGAATGGGTTAGAATAGGTTTCTCTGCAACTACAGGAGAATTGGTTGAAACACATGATATCATTAATTGGGGTGTTAAATAA

>MsG0580025598.01.T04

ATGTTGGGTCTATTGTTTCCCTTGAATATGCACAGTGGCCTATTAATTTTGTGCCAAGAAATGCTTTAG

>MsG0580025598.01.T01

ATGGTTCTCTCCAACTTAAAATCTAACAGAATTCTTTCTTCATCACTCATTACAATCTTCATCATTTCCCTCTTCCTACAATACCACAATGTCAAATCACAATCATCGTGGCAATCACGACAAGTACCACAATCAGAAACCATTGCTTTCAGCATAACAAAATTCGAGAAAGAAAATCTCGACATATTTTTCAGGGGTGACACCTCAATCTCCGGCGGCATTCTAAGACTAACAAAAACCGACCAATCTGGTAAACCACTACCAAATACCATTGGACGTGTGACATACCTCACACCAATTCATATTTGGGATAAAACTAGTGGAGAGCTAGCTGATTTCTCCACTAGTTTCTCCTTTATCGTGAACACAAACGGTTCAGATCTCCATGGAGACGGATTCGCTTTCTATATGGGACCGCTACATTTTGAAGTACCAAAAAATTCAAGCGGAGGTTATCTTGGTCTTTTCGACCCGGAAAATGCATTTCCTCCATCCAAAAACCCAATATTAGCTATTGAGTTTGATGGTTTTACAAACGAGTGGGATCCTCCTTCATCATTTCAATCTCCTCACATTGGAATTGATGTTGGGTCTATTGTTTCCCTTGAATATGCACAGTGGCCTATTAATTTTGTGCCAAGAAATGCTTTAGGAGAAGCTAGTATAAACTATAACTCTGAATCTAAAAGATTAAGTGTGTTTGTGGCTTATCCTGGGACTCAGTGGAATTCCACTAGCGTTTCAGTTGTTGTTGATCTAAGGAGTGTTTTGCCTGAATGGGTTAGAATAGGTTTCTCTGCAACTACAGGAGAATTGGTTGAAACACATGATATCATTAATTGGGGTGTTAAATAA

Protein sequence

>MsG0580025598.01.T03

MVLQTSGILLHHFNLLTLELMLGLLFPLNMHSGLLILCQEML*

>MsG0580025598.01.T02

MGPLHFEVPKNSSGGYLGLFDPENAFPPSKNPILAIEFDGFTNEWDPPSSFQSPHIGIDVGSIVSLEYAQWPINFVPRNALGEASINYNSESKRLSVFVAYPGTQWNSTSVSVVVDLRSVLPEWVRIGFSATTGELVETHDIINWGVK*

>MsG0580025598.01.T04

MLGLLFPLNMHSGLLILCQEML*

>MsG0580025598.01.T01

MVLSNLKSNRILSSSLITIFIISLFLQYHNVKSQSSWQSRQVPQSETIAFSITKFEKENLDIFFRGDTSISGGILRLTKTDQSGKPLPNTIGRVTYLTPIHIWDKTSGELADFSTSFSFIVNTNGSDLHGDGFAFYMGPLHFEVPKNSSGGYLGLFDPENAFPPSKNPILAIEFDGFTNEWDPPSSFQSPHIGIDVGSIVSLEYAQWPINFVPRNALGEASINYNSESKRLSVFVAYPGTQWNSTSVSVVVDLRSVLPEWVRIGFSATTGELVETHDIINWGVK*