AlfalfaGEDB | MsG0180005080.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005080.01

KEGG: -
GO: GO:0030246
PFAM: PF00139

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005080.01.T01	XP_013469340.2	69.455	275	80	2	1	272	1	274	1.47E-127	374

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005080.01.T01	Q41160	54.182	275	117	4	5	273	1	272	8.80E-96	285

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005080.01.T01	A0A072VPS3	69.455	275	80	2	1	272	1	274	3.14e-127	372

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048509.01	MsG0180005080.01	0.822707	2.051342e-53	1.730181e-50
MsG0180003375.01	MsG0180005080.01	0.811685	6.187088e-51	3.854718e-48
MsG0180005059.01	MsG0180005080.01	0.948194	1.527992e-106	2.810527e-101
MsG0180005060.01	MsG0180005080.01	0.858185	9.796282e-63	2.479119e-59
MsG0180005080.01	MsG0180005082.01	0.863177	2.998678e-64	9.008603e-61
MsG0180005080.01	MsG0280007689.01	0.825706	4.050263e-54	3.720130e-51
MsG0180005080.01	MsG0280008226.01	0.819551	1.094662e-52	8.450153e-50
MsG0180005080.01	MsG0280010425.01	0.829629	4.624864e-55	4.759966e-52
MsG0180005080.01	MsG0280010623.01	0.801145	1.033904e-48	4.901322e-46
MsG0180005080.01	MsG0380012889.01	0.805669	1.194007e-49	6.354622e-47
MsG0180005080.01	MsG0380013213.01	0.827782	1.293755e-54	1.261448e-51
MsG0180005080.01	MsG0380013214.01	0.825564	4.376649e-54	4.003491e-51
MsG0180005080.01	MsG0380013226.01	0.828386	9.254186e-55	9.184789e-52
MsG0180005080.01	MsG0380013239.01	0.813912	2.012685e-51	1.331335e-48
MsG0180005080.01	MsG0380016372.01	0.806154	9.441022e-50	5.087410e-47
MsG0180005080.01	MsG0380017901.01	0.818973	1.482282e-52	1.126183e-49
MsG0180005080.01	MsG0480018439.01	0.814294	1.657373e-51	1.107802e-48
MsG0180005080.01	MsG0480018559.01	0.838025	3.674513e-57	4.860492e-54
MsG0180005080.01	MsG0480018617.01	0.800568	1.355933e-48	6.334392e-46
MsG0180005080.01	MsG0480018622.01	0.806010	1.012375e-49	5.435458e-47
MsG0180005080.01	MsG0480019666.01	0.808159	3.550955e-50	2.015598e-47
MsG0180005080.01	MsG0480020904.01	0.827386	1.610417e-54	1.552553e-51
MsG0180005080.01	MsG0480021129.01	0.800231	1.587989e-48	7.355321e-46
MsG0180005080.01	MsG0480021133.01	0.803492	3.397963e-49	1.709936e-46
MsG0180005080.01	MsG0480021234.01	0.804650	1.951513e-49	1.011720e-46
MsG0180005080.01	MsG0480022833.01	0.800060	1.720475e-48	7.934597e-46
MsG0180005080.01	MsG0480023322.01	0.804350	2.253547e-49	1.159305e-46
MsG0180005080.01	MsG0480023432.01	0.806762	7.026688e-50	3.845644e-47
MsG0180005080.01	MsG0580024980.01	0.801532	8.612672e-49	4.122888e-46
MsG0180005080.01	MsG0580025007.01	0.833468	5.242041e-56	6.042169e-53
MsG0180005080.01	MsG0580025023.01	0.814029	1.896760e-51	1.258693e-48
MsG0180005080.01	MsG0580025024.01	0.855947	4.481887e-62	1.052473e-58
MsG0180005080.01	MsG0580025193.01	0.811469	6.893587e-51	4.270713e-48
MsG0180005080.01	MsG0580026311.01	0.815245	1.020500e-51	6.998162e-49
MsG0180005080.01	MsG0580029938.01	0.805339	1.400412e-49	7.389998e-47
MsG0180005080.01	MsG0680032461.01	0.810161	1.322909e-50	7.916336e-48
MsG0180005080.01	MsG0680032932.01	0.808800	2.592072e-50	1.496111e-47
MsG0180005080.01	MsG0680034254.01	0.803313	3.700285e-49	1.853446e-46
MsG0180005080.01	MsG0680034354.01	0.806878	6.643015e-50	3.646578e-47
MsG0180005080.01	MsG0680034974.01	0.803122	4.052699e-49	2.020053e-46
MsG0180005080.01	MsG0680034978.01	0.811307	7.473116e-51	4.609714e-48
MsG0180005080.01	MsG0780036376.01	0.822689	2.070680e-53	1.745616e-50
MsG0180005080.01	MsG0780038307.01	0.827636	1.402016e-54	1.361216e-51
MsG0180005080.01	MsG0780039923.01	0.850504	1.627531e-60	3.194481e-57
MsG0180005080.01	MsG0780039979.01	0.805264	1.451856e-49	7.646433e-47
MsG0180005080.01	MsG0780040716.01	0.804245	2.369360e-49	1.215553e-46
MsG0180005080.01	MsG0780040991.01	0.803457	3.454040e-49	1.736597e-46
MsG0180005080.01	MsG0780041248.01	0.802809	4.703269e-49	2.325940e-46
MsG0180005080.01	MsG0880042633.01	0.806054	9.908464e-50	5.325776e-47
MsG0180005080.01	MsG0880042635.01	0.817344	3.462097e-52	2.514786e-49
MsG0180005080.01	MsG0880042642.01	0.830718	2.507592e-55	2.663903e-52
MsG0180005080.01	MsG0880043550.01	0.802910	4.483349e-49	2.222768e-46
MsG0180005080.01	MsG0880043953.01	0.818636	1.767280e-52	1.330082e-49
MsG0180005080.01	MsG0880045854.01	0.826008	3.433844e-54	3.181256e-51
MsG0180005080.01	MsG0880047450.01	0.807514	4.870661e-50	2.718313e-47

PPI

Gene1	Gene2	Type
MsG0180004091.01	MsG0180005080.01	PPI
MsG0380014272.01	MsG0180005080.01	PPI
MsG0180004091.01	MsG0180005080.01	PPI
MsG0380013594.01	MsG0180005080.01	PPI
MsG0180004091.01	MsG0180005080.01	PPI
MsG0880045216.01	MsG0180005080.01	PPI
MsG0180005080.01	MsG0880045214.01	PPI
MsG0180005082.01	MsG0180005080.01	PPI
MsG0180004091.01	MsG0180005080.01	PPI
MsG0380013595.01	MsG0180005080.01	PPI
MsG0180005080.01	MsG0180004091.01	PPI
MsG0180005080.01	MsG0580025598.01	PPI
MsG0380014748.01	MsG0180005080.01	PPI
MsG0580025598.01	MsG0180005080.01	PPI
MsG0880045214.01	MsG0180005080.01	PPI
MsG0180004091.01	MsG0180005080.01	PPI
MsG0180005060.01	MsG0180005080.01	PPI
MsG0580025591.01	MsG0180005080.01	PPI
MsG0180005080.01	MsG0380013593.01	PPI
MsG0380014275.01	MsG0180005080.01	PPI
MsG0580025592.01	MsG0180005080.01	PPI
MsG0880045217.01	MsG0180005080.01	PPI
MsG0380014744.01	MsG0180005080.01	PPI
MsG0880042846.01	MsG0180005080.01	PPI
MsG0180005059.01	MsG0180005080.01	PPI
MsG0380013593.01	MsG0180005080.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005080.01.T01	MTR_1g090973	69.455	275	80	2	1	272	1	274	7.97e-131	372
MsG0180005080.01.T01	MTR_1g090960	61.922	281	61	4	1	272	1	244	8.26e-109	315
MsG0180005080.01.T01	MTR_3g054170	56.432	241	102	2	21	261	17	254	3.91e-92	273
MsG0180005080.01.T01	MTR_0163s0020	50.714	280	129	3	1	273	2	279	1.89e-90	270
MsG0180005080.01.T01	MTR_0684s0020	50.714	280	129	3	1	273	1	278	2.17e-90	269
MsG0180005080.01.T01	MTR_3g047140	52.963	270	125	2	1	269	1	269	2.10e-89	267
MsG0180005080.01.T01	MTR_3g054200	53.942	241	108	2	21	261	17	254	3.64e-87	260
MsG0180005080.01.T01	MTR_3g054180	53.498	243	108	3	21	261	21	260	4.53e-84	253
MsG0180005080.01.T01	MTR_1g090943	49.603	252	123	3	14	264	15	263	3.85e-71	220
MsG0180005080.01.T01	MTR_3g027280	47.464	276	127	11	1	264	1	270	3.49e-66	207
MsG0180005080.01.T01	MTR_1g090957	43.706	286	133	11	1	275	1	269	1.09e-58	188
MsG0180005080.01.T01	MTR_1g090937	42.336	274	139	10	1	265	1	264	1.42e-56	183
MsG0180005080.01.T01	MTR_1g090930	41.516	277	141	10	1	265	1	268	6.24e-52	171
MsG0180005080.01.T01	MTR_8g068040	40.755	265	129	11	14	262	10	262	1.56e-47	159
MsG0180005080.01.T01	MTR_8g068030	39.630	270	129	12	14	262	10	266	3.96e-46	155
MsG0180005080.01.T01	MTR_5g031160	37.847	288	139	13	6	261	8	287	2.57e-44	152
MsG0180005080.01.T01	MTR_5g031120	40.562	249	119	11	35	261	38	279	1.61e-42	147
MsG0180005080.01.T01	MTR_5g031100	39.919	248	121	10	35	261	38	278	2.29e-42	146
MsG0180005080.01.T01	MTR_5g031140	39.200	250	124	11	33	261	44	286	6.02e-41	143
MsG0180005080.01.T01	MTR_4g056320	36.567	268	126	7	2	265	6	233	8.81e-41	142
MsG0180005080.01.T01	MTR_8g067980	39.662	237	126	8	36	259	25	257	1.42e-40	141
MsG0180005080.01.T01	MTR_8g023510	38.806	268	148	10	1	261	1	259	2.06e-39	139
MsG0180005080.01.T01	MTR_8g068050	37.132	272	150	10	11	269	18	281	6.90e-39	144
MsG0180005080.01.T01	MTR_8g067990	37.553	237	131	8	36	259	25	257	1.45e-38	136
MsG0180005080.01.T01	MTR_4g023810	38.559	236	133	7	30	261	27	254	1.00e-34	126
MsG0180005080.01.T01	MTR_1g014240	38.272	243	130	9	38	264	40	278	1.99e-33	129
MsG0180005080.01.T01	MTR_4g093080	36.296	270	142	12	17	268	9	266	3.96e-33	128
MsG0180005080.01.T01	MTR_8g067960	39.732	224	119	9	49	261	23	241	4.81e-32	119
MsG0180005080.01.T01	MTR_4g056360	34.082	267	130	11	2	261	6	233	7.97e-31	116
MsG0180005080.01.T01	MTR_5g031090	34.553	246	135	11	37	261	35	275	1.77e-29	112
MsG0180005080.01.T01	MTR_8g067940	34.532	278	149	14	8	261	13	281	8.82e-29	111
MsG0180005080.01.T01	MTR_5g031030	32.922	243	143	8	35	261	36	274	1.46e-28	110
MsG0180005080.01.T01	MTR_8g067720	34.926	272	154	11	9	268	4	264	3.57e-28	113
MsG0180005080.01.T01	MTR_7g115740	32.157	255	152	9	23	262	13	261	1.45e-27	112
MsG0180005080.01.T01	MTR_7g116130	31.461	267	162	7	15	266	6	266	1.88e-27	111
MsG0180005080.01.T01	MTR_8g067690	36.975	238	136	8	35	261	60	294	2.37e-27	111
MsG0180005080.01.T01	MTR_4g093110	34.363	259	143	13	19	262	19	265	1.05e-25	106
MsG0180005080.01.T01	MTR_8g067930	34.211	266	159	9	16	268	4	266	1.22e-25	106
MsG0180005080.01.T01	MTR_8g067735	35.124	242	142	8	35	264	43	281	1.69e-25	105
MsG0180005080.01.T01	MTR_4g093140	32.819	259	147	12	19	262	19	265	1.28e-24	103
MsG0180005080.01.T01	MTR_4g093040	32.830	265	156	8	5	254	3	260	2.04e-24	102
MsG0180005080.01.T01	MTR_8g067630	34.821	224	132	7	35	247	30	250	5.07e-23	98.6
MsG0180005080.01.T01	MTR_4g093070	35.354	198	114	6	77	262	72	267	1.03e-22	97.8
MsG0180005080.01.T01	MTR_3g463370	29.368	269	136	7	11	269	6	230	3.11e-20	87.4
MsG0180005080.01.T01	MTR_4g093040	37.569	181	95	6	87	254	3	178	5.18e-20	89.7
MsG0180005080.01.T01	MTR_4g093050	32.365	241	145	11	36	260	27	265	1.71e-18	85.5
MsG0180005080.01.T01	MTR_5g068770	32.389	247	135	11	38	259	38	277	3.16e-17	81.6
MsG0180005080.01.T01	MTR_1g048360	29.744	195	117	6	77	259	74	260	4.00e-15	75.5
MsG0180005080.01.T01	MTR_7g115740	30.519	154	98	5	23	170	13	163	1.21e-14	73.9
MsG0180005080.01.T01	MTR_5g077100	32.258	217	124	8	59	263	49	254	3.39e-13	69.7
MsG0180005080.01.T01	MTR_7g062940	28.571	266	149	11	22	260	7	258	3.98e-13	69.7
MsG0180005080.01.T01	MTR_5g025020	28.000	200	119	7	76	255	87	281	9.30e-13	68.6
MsG0180005080.01.T01	MTR_2g090250	29.688	256	150	11	41	272	29	278	2.71e-11	63.9
MsG0180005080.01.T01	MTR_7g062890	28.044	271	167	10	16	265	1	264	3.12e-11	63.9
MsG0180005080.01.T01	MTR_0015s0030	32.161	199	112	10	73	257	62	251	8.15e-11	62.8
MsG0180005080.01.T01	MTR_7g063010	31.646	237	123	11	50	260	36	259	8.78e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005080.01.T01	AT5G10530	40.244	246	120	13	35	262	19	255	5.54e-32	124
MsG0180005080.01.T01	AT5G65600	34.081	223	128	9	35	241	35	254	7.61e-23	98.2
MsG0180005080.01.T01	AT5G06740	31.179	263	139	12	22	258	23	269	1.47e-17	82.8
MsG0180005080.01.T01	AT5G06740	31.179	263	139	12	22	258	11	257	1.47e-17	82.8
MsG0180005080.01.T01	AT5G55830	31.390	223	130	8	57	260	49	267	8.96e-16	77.4
MsG0180005080.01.T01	AT5G03140	29.762	252	159	9	17	255	11	257	3.96e-14	72.8
MsG0180005080.01.T01	AT4G02410	28.302	265	151	11	29	266	20	272	5.63e-14	72.0
MsG0180005080.01.T01	AT3G53810	28.287	251	157	7	29	260	18	264	7.65e-13	68.9
MsG0180005080.01.T01	AT4G04960	30.172	232	130	8	36	244	21	243	8.38e-13	68.6
MsG0180005080.01.T01	AT4G04960	30.172	232	130	9	36	244	21	243	1.02e-12	68.6
MsG0180005080.01.T01	AT4G02420	28.195	266	161	11	17	260	6	263	1.54e-12	67.8
MsG0180005080.01.T01	AT5G01560	30.000	250	142	10	38	259	30	274	6.00e-12	66.2
MsG0180005080.01.T01	AT2G37710	26.000	250	160	8	31	260	20	264	6.58e-12	65.9
MsG0180005080.01.T01	AT4G29050	27.753	227	128	9	60	260	59	275	1.26e-11	65.1
MsG0180005080.01.T01	AT4G29050	27.753	227	128	9	60	260	45	261	1.29e-11	65.1

Find 75 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTCCAACATGTGTGTATT+TGG	0.093315	1:+86666934	None:intergenic
GAAAAGCTTGCAATATTATT+TGG	0.099570	1:+86666632	None:intergenic
TTTCTTTAAAGAAAATAAAA+TGG	0.247146	1:-86666895	MsG0180005080.01.T01:CDS
AAAAGCTTGCAATATTATTT+GGG	0.253456	1:+86666633	None:intergenic
ACTTCCTTGTAGGGAAATTA+TGG	0.265257	1:+86667285	None:intergenic
CCCAACAGATCCAAACTATT+TGG	0.303319	1:-86667224	MsG0180005080.01.T01:CDS
TAAAGAAAATAAAATGGAAT+AGG	0.318424	1:-86666889	MsG0180005080.01.T01:CDS
TGACTAACACAACAGAAAAT+AGG	0.328825	1:+86667401	None:intergenic
ATTCCAACATGTGTGTATTT+GGG	0.336887	1:+86666935	None:intergenic
AGCTGAGTTGCTAGGAATAT+TGG	0.342652	1:+86667045	None:intergenic
CATCAGATGTGACAAACTTT+TGG	0.346785	1:+86667101	None:intergenic
ATTCAGCACCAGTGCCCATT+TGG	0.349343	1:-86667186	MsG0180005080.01.T01:CDS
GCCCGACCAGCCAAATAGTT+TGG	0.378898	1:+86667214	None:intergenic
GCCAAATAGTTTGGATCTGT+TGG	0.383215	1:+86667223	None:intergenic
GCCAGTGGTTTCATCCCAAA+TGG	0.385140	1:+86667171	None:intergenic
TGGGCACTGGTGCTGAATAC+AGG	0.400885	1:+86667191	None:intergenic
AAATTGAGGCACTTCCTTGT+AGG	0.404967	1:+86667275	None:intergenic
GGATTTACTATTACTGAAGT+TGG	0.409756	1:+86667306	None:intergenic
AAAGAAAATAAAATGGAATA+GGG	0.416250	1:-86666888	MsG0180005080.01.T01:CDS
AGCTAAACAACCTCCATTCA+TGG	0.418791	1:-86666685	MsG0180005080.01.T01:CDS
TTGACTTGAAAGACCATGAA+TGG	0.422733	1:+86666672	None:intergenic
TTCAGCACCAGTGCCCATTT+GGG	0.426698	1:-86667185	MsG0180005080.01.T01:CDS
CAAAAGTTTGTCACATCTGA+TGG	0.426781	1:-86667100	MsG0180005080.01.T01:CDS
AATTGAGGCACTTCCTTGTA+GGG	0.429899	1:+86667276	None:intergenic
GTCGATCAAACCAAATCATA+TGG	0.434983	1:+86666607	None:intergenic
AAACCAATCCTTACGGTGTT+CGG	0.435951	1:+86666737	None:intergenic
ATAGTTGAAAAGGTACCATC+TGG	0.449708	1:+86666791	None:intergenic
TCCGCATACAATCCATTTGT+TGG	0.449749	1:-86666992	MsG0180005080.01.T01:CDS
GTATTTGGGATCCCACCTAT+TGG	0.452286	1:+86666949	None:intergenic
CCAAATAGTTTGGATCTGTT+GGG	0.452907	1:+86667224	None:intergenic
TCCAAACTATTTGGCTGGTC+GGG	0.455927	1:-86667215	MsG0180005080.01.T01:CDS
ATGGTCTTTCAAGTCAAACT+TGG	0.459323	1:-86666666	MsG0180005080.01.T01:CDS
AGTTTGATAGTTATGCCAAT+AGG	0.461031	1:-86666964	MsG0180005080.01.T01:CDS
CTTCCGAACACCGTAAGGAT+TGG	0.469222	1:-86666740	MsG0180005080.01.T01:CDS
TCAAACTCTACACCAACAAA+TGG	0.477349	1:+86666980	None:intergenic
GACTTGAGCAATAGTTGAAA+AGG	0.492838	1:+86666781	None:intergenic
TGTCTTAGAGAGATTGTCAT+AGG	0.497716	1:+86666835	None:intergenic
ATAAAATGGAATAGGGTGAA+TGG	0.502888	1:-86666881	MsG0180005080.01.T01:CDS
AACTCAGCTGGTGGAAACCT+TGG	0.506073	1:-86667031	MsG0180005080.01.T01:CDS
GGCTATATCCAAGATTCCAT+TGG	0.512019	1:+86667249	None:intergenic
GATTCCATTGGATAAAATTG+AGG	0.512081	1:+86667261	None:intergenic
ATCCAAACTATTTGGCTGGT+CGG	0.513232	1:-86667216	MsG0180005080.01.T01:CDS
CCAGTGGTTTCATCCCAAAT+GGG	0.521697	1:+86667172	None:intergenic
TTCATTTCTCTTGTTAGCAA+CGG	0.523374	1:-86667368	MsG0180005080.01.T01:CDS
ATGGAGGTTGTTTAGCTGAC+CGG	0.532538	1:+86666691	None:intergenic
TTTCCACCAGCTGAGTTGCT+AGG	0.536666	1:+86667037	None:intergenic
GGAATATTGGCTCGATCTTG+TGG	0.539230	1:+86667058	None:intergenic
ACAGATCCAAACTATTTGGC+TGG	0.541475	1:-86667220	MsG0180005080.01.T01:CDS
TTGATAGTTATGCCAATAGG+TGG	0.543485	1:-86666961	MsG0180005080.01.T01:CDS
ATAGTTTGGATCTGTTGGGT+TGG	0.547533	1:+86667228	None:intergenic
GAATAGGGTGAATGGCGCCT+TGG	0.550930	1:-86666873	MsG0180005080.01.T01:CDS
GCTTGCAATATTATTTGGGG+TGG	0.556881	1:+86666637	None:intergenic
GGGCACTGGTGCTGAATACA+GGG	0.558962	1:+86667192	None:intergenic
AAAGCTTGCAATATTATTTG+GGG	0.560702	1:+86666634	None:intergenic
CAGAGCTTCCGAACACCGTA+AGG	0.561999	1:-86666745	MsG0180005080.01.T01:CDS
CCCATTTGGGATGAAACCAC+TGG	0.562569	1:-86667172	MsG0180005080.01.T01:CDS
GTTTCATCCCAAATGGGCAC+TGG	0.563265	1:+86667178	None:intergenic
GCTGCCACATCAAGAGGTGC+CGG	0.566269	1:-86666710	MsG0180005080.01.T01:CDS
GTCATGCACTCTGAATGAAA+AGG	0.571753	1:+86667126	None:intergenic
GATCCCAAATACACACATGT+TGG	0.576936	1:-86666938	MsG0180005080.01.T01:CDS
AAATCCATAATTTCCCTACA+AGG	0.579177	1:-86667289	MsG0180005080.01.T01:CDS
ACTGAAGTTGGTGATGTCGA+AGG	0.592142	1:+86667318	None:intergenic
GAAGCTCGCCACGTTGCCAG+TGG	0.595816	1:+86667156	None:intergenic
AATATTCCTAGCAACTCAGC+TGG	0.601298	1:-86667043	MsG0180005080.01.T01:CDS
ATTCCTAGCAACTCAGCTGG+TGG	0.607983	1:-86667040	MsG0180005080.01.T01:CDS
TGATAGTTATGCCAATAGGT+GGG	0.619156	1:-86666960	MsG0180005080.01.T01:CDS
ATTTGTTGGAAAGAAAGCCA+TGG	0.619705	1:+86667429	None:intergenic
GTGGTTGTATCTTACCCAGA+TGG	0.625936	1:-86666806	MsG0180005080.01.T01:CDS
CTGACCGGCACCTCTTGATG+TGG	0.630323	1:+86666706	None:intergenic
TAGTTGAAAAGGTACCATCT+GGG	0.650948	1:+86666792	None:intergenic
TCTCTCTAAGACATTGAATG+TGG	0.660926	1:-86666825	MsG0180005080.01.T01:CDS
ACCAACAAATGGATTGTATG+CGG	0.667112	1:+86666991	None:intergenic
ACTTGAAAGACCATGAATGG+AGG	0.674593	1:+86666675	None:intergenic
GGAGATATTAACATCGACCA+AGG	0.682054	1:+86666856	None:intergenic
GGATGAAACCACTGGCAACG+TGG	0.707919	1:-86667164	MsG0180005080.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCTTTAAAGAAAATAAAA+TGG	-	Chr1:86667154-86667173	MsG0180005080.01.T01:CDS	10.0%
!!	AAAGAAAATAAAATGGAATA+GGG	-	Chr1:86667161-86667180	MsG0180005080.01.T01:CDS	15.0%
!!	TAAAGAAAATAAAATGGAAT+AGG	-	Chr1:86667160-86667179	MsG0180005080.01.T01:CDS	15.0%
!!	AAAAGCTTGCAATATTATTT+GGG	+	Chr1:86667419-86667438	None:intergenic	20.0%
!	AAAGCTTGCAATATTATTTG+GGG	+	Chr1:86667418-86667437	None:intergenic	25.0%
!	GAAAAGCTTGCAATATTATT+TGG	+	Chr1:86667420-86667439	None:intergenic	25.0%
!!	AATTTTATCCAATGGAATCT+TGG	-	Chr1:86666792-86666811	MsG0180005080.01.T01:CDS	25.0%
!!	AGTTTTCATTAACAATTCCA+AGG	+	Chr1:86667038-86667057	None:intergenic	25.0%
!!!	GAAAATAGGTTTTGATTTGT+TGG	+	Chr1:86666637-86666656	None:intergenic	25.0%
	AAATCCATAATTTCCCTACA+AGG	-	Chr1:86666760-86666779	MsG0180005080.01.T01:CDS	30.0%
	AATTCCAACATGTGTGTATT+TGG	+	Chr1:86667118-86667137	None:intergenic	30.0%
	ATAAAATGGAATAGGGTGAA+TGG	-	Chr1:86667168-86667187	MsG0180005080.01.T01:CDS	30.0%
	ATTCCAACATGTGTGTATTT+GGG	+	Chr1:86667117-86667136	None:intergenic	30.0%
	GATTCCATTGGATAAAATTG+AGG	+	Chr1:86666791-86666810	None:intergenic	30.0%
	GGATTTACTATTACTGAAGT+TGG	+	Chr1:86666746-86666765	None:intergenic	30.0%
	TGACTAACACAACAGAAAAT+AGG	+	Chr1:86666651-86666670	None:intergenic	30.0%
	TTCATTTCTCTTGTTAGCAA+CGG	-	Chr1:86666681-86666700	MsG0180005080.01.T01:CDS	30.0%
!	AGTTTGATAGTTATGCCAAT+AGG	-	Chr1:86667085-86667104	MsG0180005080.01.T01:CDS	30.0%
	ACTTCCTTGTAGGGAAATTA+TGG	+	Chr1:86666767-86666786	None:intergenic	35.0%
	AGCAGAAAAACCAATCCTTA+CGG	+	Chr1:86667322-86667341	None:intergenic	35.0%
	CAAAAGTTTGTCACATCTGA+TGG	-	Chr1:86666949-86666968	MsG0180005080.01.T01:CDS	35.0%
	CCAAATAGTTTGGATCTGTT+GGG	+	Chr1:86666828-86666847	None:intergenic	35.0%
	GACTTGAGCAATAGTTGAAA+AGG	+	Chr1:86667271-86667290	None:intergenic	35.0%
	TCAAACTCTACACCAACAAA+TGG	+	Chr1:86667072-86667091	None:intergenic	35.0%
	TGATAGTTATGCCAATAGGT+GGG	-	Chr1:86667089-86667108	MsG0180005080.01.T01:CDS	35.0%
	TGTCTTAGAGAGATTGTCAT+AGG	+	Chr1:86667217-86667236	None:intergenic	35.0%
!	AGTGCCTCAATTTTATCCAA+TGG	-	Chr1:86666784-86666803	MsG0180005080.01.T01:CDS	35.0%
!	ATGGTCTTTCAAGTCAAACT+TGG	-	Chr1:86667383-86667402	MsG0180005080.01.T01:CDS	35.0%
!	CATCAGATGTGACAAACTTT+TGG	+	Chr1:86666951-86666970	None:intergenic	35.0%
!	TTGACTTGAAAGACCATGAA+TGG	+	Chr1:86667380-86667399	None:intergenic	35.0%
!	TTGATAGTTATGCCAATAGG+TGG	-	Chr1:86667088-86667107	MsG0180005080.01.T01:CDS	35.0%
!!	ACCAACAAATGGATTGTATG+CGG	+	Chr1:86667061-86667080	None:intergenic	35.0%
!!	ATAGTTGAAAAGGTACCATC+TGG	+	Chr1:86667261-86667280	None:intergenic	35.0%
!!	TAGTTGAAAAGGTACCATCT+GGG	+	Chr1:86667260-86667279	None:intergenic	35.0%
!!	TCTCTCTAAGACATTGAATG+TGG	-	Chr1:86667224-86667243	MsG0180005080.01.T01:CDS	35.0%
!!!	GAAGGAAACTGTTTTTTGTG+AGG	+	Chr1:86666716-86666735	None:intergenic	35.0%
	AAACCAATCCTTACGGTGTT+CGG	+	Chr1:86667315-86667334	None:intergenic	40.0%
	AATATTCCTAGCAACTCAGC+TGG	-	Chr1:86667006-86667025	MsG0180005080.01.T01:CDS	40.0%
	ACAGATCCAAACTATTTGGC+TGG	-	Chr1:86666829-86666848	MsG0180005080.01.T01:CDS	40.0%
	ACTTGAAAGACCATGAATGG+AGG	+	Chr1:86667377-86667396	None:intergenic	40.0%
	AGCTAAACAACCTCCATTCA+TGG	-	Chr1:86667364-86667383	MsG0180005080.01.T01:CDS	40.0%
	AGCTGAGTTGCTAGGAATAT+TGG	+	Chr1:86667007-86667026	None:intergenic	40.0%
	ATAGTTTGGATCTGTTGGGT+TGG	+	Chr1:86666824-86666843	None:intergenic	40.0%
	ATCCAAACTATTTGGCTGGT+CGG	-	Chr1:86666833-86666852	MsG0180005080.01.T01:CDS	40.0%
	CCCAACAGATCCAAACTATT+TGG	-	Chr1:86666825-86666844	MsG0180005080.01.T01:CDS	40.0%
	GATCCCAAATACACACATGT+TGG	-	Chr1:86667111-86667130	MsG0180005080.01.T01:CDS	40.0%
	GCCAAATAGTTTGGATCTGT+TGG	+	Chr1:86666829-86666848	None:intergenic	40.0%
	GCTTGCAATATTATTTGGGG+TGG	+	Chr1:86667415-86667434	None:intergenic	40.0%
	GGAGATATTAACATCGACCA+AGG	+	Chr1:86667196-86667215	None:intergenic	40.0%
	GGCTATATCCAAGATTCCAT+TGG	+	Chr1:86666803-86666822	None:intergenic	40.0%
	GTCATGCACTCTGAATGAAA+AGG	+	Chr1:86666926-86666945	None:intergenic	40.0%
	TCCGCATACAATCCATTTGT+TGG	-	Chr1:86667057-86667076	MsG0180005080.01.T01:CDS	40.0%
!	AAATTGAGGCACTTCCTTGT+AGG	+	Chr1:86666777-86666796	None:intergenic	40.0%
!	AATTGAGGCACTTCCTTGTA+GGG	+	Chr1:86666776-86666795	None:intergenic	40.0%
	ATGGAGGTTGTTTAGCTGAC+CGG	+	Chr1:86667361-86667380	None:intergenic	45.0%
	CCAGTGGTTTCATCCCAAAT+GGG	+	Chr1:86666880-86666899	None:intergenic	45.0%
	GTGGTTGTATCTTACCCAGA+TGG	-	Chr1:86667243-86667262	MsG0180005080.01.T01:CDS	45.0%
!	GTATTTGGGATCCCACCTAT+TGG	+	Chr1:86667103-86667122	None:intergenic	45.0%
!	TCCAAACTATTTGGCTGGTC+GGG	-	Chr1:86666834-86666853	MsG0180005080.01.T01:CDS	45.0%
!	TTTTCTGCTGCCACATCAAG+AGG	-	Chr1:86667333-86667352	MsG0180005080.01.T01:CDS	45.0%
!!	ACTGAAGTTGGTGATGTCGA+AGG	+	Chr1:86666734-86666753	None:intergenic	45.0%
!!	GGAATATTGGCTCGATCTTG+TGG	+	Chr1:86666994-86667013	None:intergenic	45.0%
	AACTCAGCTGGTGGAAACCT+TGG	-	Chr1:86667018-86667037	MsG0180005080.01.T01:CDS	50.0%
	ATTCAGCACCAGTGCCCATT+TGG	-	Chr1:86666863-86666882	MsG0180005080.01.T01:CDS	50.0%
	ATTCCTAGCAACTCAGCTGG+TGG	-	Chr1:86667009-86667028	MsG0180005080.01.T01:CDS	50.0%
	CTTCCGAACACCGTAAGGAT+TGG	-	Chr1:86667309-86667328	MsG0180005080.01.T01:CDS	50.0%
	GCCAGTGGTTTCATCCCAAA+TGG	+	Chr1:86666881-86666900	None:intergenic	50.0%
	GTTTCATCCCAAATGGGCAC+TGG	+	Chr1:86666874-86666893	None:intergenic	50.0%
	TTCAGCACCAGTGCCCATTT+GGG	-	Chr1:86666864-86666883	MsG0180005080.01.T01:CDS	50.0%
	TTTCCACCAGCTGAGTTGCT+AGG	+	Chr1:86667015-86667034	None:intergenic	50.0%
!	CCCATTTGGGATGAAACCAC+TGG	-	Chr1:86666877-86666896	MsG0180005080.01.T01:CDS	50.0%
	CAGAGCTTCCGAACACCGTA+AGG	-	Chr1:86667304-86667323	MsG0180005080.01.T01:CDS	55.0%
	GAATAGGGTGAATGGCGCCT+TGG	-	Chr1:86667176-86667195	MsG0180005080.01.T01:CDS	55.0%
	GCCCGACCAGCCAAATAGTT+TGG	+	Chr1:86666838-86666857	None:intergenic	55.0%
	GGATGAAACCACTGGCAACG+TGG	-	Chr1:86666885-86666904	MsG0180005080.01.T01:CDS	55.0%
!!	GGGCACTGGTGCTGAATACA+GGG	+	Chr1:86666860-86666879	None:intergenic	55.0%
!!	TGGGCACTGGTGCTGAATAC+AGG	+	Chr1:86666861-86666880	None:intergenic	55.0%
	CTGACCGGCACCTCTTGATG+TGG	+	Chr1:86667346-86667365	None:intergenic	60.0%
!	GCTGCCACATCAAGAGGTGC+CGG	-	Chr1:86667339-86667358	MsG0180005080.01.T01:CDS	60.0%
	GAAGCTCGCCACGTTGCCAG+TGG	+	Chr1:86666896-86666915	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	86666622	86667449	86666622	ID=MsG0180005080.01;Name=MsG0180005080.01
Chr1	mRNA	86666622	86667449	86666622	ID=MsG0180005080.01.T01;Parent=MsG0180005080.01;Name=MsG0180005080.01.T01;_AED=0.14;_eAED=0.14;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|275
Chr1	exon	86666622	86667449	86666622	ID=MsG0180005080.01.T01:exon:16888;Parent=MsG0180005080.01.T01
Chr1	CDS	86666622	86667449	86666622	ID=MsG0180005080.01.T01:cds;Parent=MsG0180005080.01.T01
Chr1	mRNA	86666987	86667130	86666987	ID=MsG0180005080.01.T02;Parent=MsG0180005080.01;Name=MsG0180005080.01.T02;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|47
Chr1	exon	86666987	86667130	86666987	ID=MsG0180005080.01.T02:exon:16889;Parent=MsG0180005080.01.T02
Chr1	CDS	86666987	86667130	86666987	ID=MsG0180005080.01.T02:cds;Parent=MsG0180005080.01.T02
Chr1	mRNA	86666771	86666830	86666771	ID=MsG0180005080.01.T03;Parent=MsG0180005080.01;Name=MsG0180005080.01.T03;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|19
Chr1	exon	86666771	86666830	86666771	ID=MsG0180005080.01.T03:exon:16890;Parent=MsG0180005080.01.T03
Chr1	CDS	86666771	86666830	86666771	ID=MsG0180005080.01.T03:cds;Parent=MsG0180005080.01.T03

Gene Sequence

>MsG0180005080.01.T01

ATGGCTTTCTTTCCAACAAATCAAAACCTATTTTCTGTTGTGTTAGTCATTTTCACAACTTCATTTCTCTTGTTAGCAACGGAACTAGTGACCTCACAAAAAACAGTTTCCTTCGACATCACCAACTTCAGTAATAGTAAATCCATAATTTCCCTACAAGGAAGTGCCTCAATTTTATCCAATGGAATCTTGGATATAGCCAACCCAACAGATCCAAACTATTTGGCTGGTCGGGCCCTGTATTCAGCACCAGTGCCCATTTGGGATGAAACCACTGGCAACGTGGCGAGCTTCGTCACTTCCTTTTCATTCAGAGTGCATGACTACCAAAAGTTTGTCACATCTGATGGATTTGTTTTCTTTCTTGTACCACAAGATCGAGCCAATATTCCTAGCAACTCAGCTGGTGGAAACCTTGGAATTGTTAATGAAAACTCCGCATACAATCCATTTGTTGGTGTAGAGTTTGATAGTTATGCCAATAGGTGGGATCCCAAATACACACATGTTGGAATTAATGCAAACTCTTTGATTTCTTTAAAGAAAATAAAATGGAATAGGGTGAATGGCGCCTTGGTCGATGTTAATATCTCCTATGACAATCTCTCTAAGACATTGAATGTGGTTGTATCTTACCCAGATGGTACCTTTTCAACTATTGCTCAAGTCATTGATTTGAAAGCAGAGCTTCCGAACACCGTAAGGATTGGTTTTTCTGCTGCCACATCAAGAGGTGCCGGTCAGCTAAACAACCTCCATTCATGGTCTTTCAAGTCAAACTTGGACACAACCACCCCAAATAATATTGCAAGCTTTTCAGCCATATGA

>MsG0180005080.01.T03

ATGTGGTTGTATCTTACCCAGATGGTACCTTTTCAACTATTGCTCAAGTCATTGATTTGA

>MsG0180005080.01.T02

ATGACTACCAAAAGTTTGTCACATCTGATGGATTTGTTTTCTTTCTTGTACCACAAGATCGAGCCAATATTCCTAGCAACTCAGCTGGTGGAAACCTTGGAATTGTTAATGAAAACTCCGCATACAATCCATTTGTTGGTGTAG

Protein sequence

>MsG0180005080.01.T01

MAFFPTNQNLFSVVLVIFTTSFLLLATELVTSQKTVSFDITNFSNSKSIISLQGSASILSNGILDIANPTDPNYLAGRALYSAPVPIWDETTGNVASFVTSFSFRVHDYQKFVTSDGFVFFLVPQDRANIPSNSAGGNLGIVNENSAYNPFVGVEFDSYANRWDPKYTHVGINANSLISLKKIKWNRVNGALVDVNISYDNLSKTLNVVVSYPDGTFSTIAQVIDLKAELPNTVRIGFSAATSRGAGQLNNLHSWSFKSNLDTTTPNNIASFSAI*

>MsG0180005080.01.T03

MWLYLTQMVPFQLLLKSLI*

>MsG0180005080.01.T02

MTTKSLSHLMDLFSFLYHKIEPIFLATQLVETLELLMKTPHTIHLLV*