Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014744.01.T01 | XP_024634278.1 | 93.165 | 278 | 18 | 1 | 1 | 277 | 1 | 278 | 1.03E-177 | 501 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014744.01.T01 | Q01807 | 92.086 | 278 | 21 | 1 | 1 | 277 | 1 | 278 | 5.96E-179 | 496 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014744.01.T01 | O49899 | 92.086 | 278 | 22 | 0 | 1 | 278 | 1 | 278 | 1.60e-176 | 497 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003444.01 | MsG0380014744.01 | 0.804019 | 2.641134e-49 | 1.347224e-46 |
| MsG0180004091.01 | MsG0380014744.01 | 0.803414 | 3.526172e-49 | 1.770893e-46 |
| MsG0380014744.01 | MsG0380014748.01 | 0.885380 | 8.546519e-72 | 5.853887e-68 |
| MsG0380014744.01 | MsG0380017177.01 | 0.805475 | 1.311150e-49 | 6.943259e-47 |
| MsG0380014744.01 | MsG0480020242.01 | 0.803413 | 3.527283e-49 | 1.771416e-46 |
| MsG0380014744.01 | MsG0480022094.01 | 0.823001 | 1.752036e-53 | 1.490114e-50 |
| MsG0380014744.01 | MsG0480022115.01 | 0.819260 | 1.274966e-52 | 9.762755e-50 |
| MsG0380014744.01 | MsG0580024157.01 | 0.807777 | 4.282593e-50 | 2.406464e-47 |
| MsG0380014744.01 | MsG0580027261.01 | 0.831614 | 1.510360e-55 | 1.647690e-52 |
| MsG0380014744.01 | MsG0680031877.01 | 0.804957 | 1.683169e-49 | 8.796066e-47 |
| MsG0380014744.01 | MsG0780040488.01 | 0.803921 | 2.767071e-49 | 1.407989e-46 |
| MsG0380014744.01 | MsG0780041764.01 | 0.828283 | 9.798902e-55 | 9.695469e-52 |
| MsG0380014744.01 | MsG0880044237.01 | 0.809823 | 1.564017e-50 | 9.277102e-48 |
| MsG0380014744.01 | MsG0880046762.01 | 0.821206 | 4.566955e-53 | 3.691818e-50 |
| MsG0280009514.01 | MsG0380014744.01 | 0.815705 | 8.061552e-52 | 5.599064e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014744.01.T01 | MTR_3g463370 | 79.856 | 278 | 16 | 2 | 1 | 277 | 1 | 239 | 1.13e-152 | 426 |
| MsG0380014744.01.T01 | MTR_8g023510 | 70.301 | 266 | 69 | 4 | 1 | 263 | 3 | 261 | 1.39e-123 | 353 |
| MsG0380014744.01.T01 | MTR_4g023810 | 56.846 | 241 | 94 | 3 | 22 | 262 | 25 | 255 | 1.20e-92 | 275 |
| MsG0380014744.01.T01 | MTR_8g068040 | 38.491 | 265 | 138 | 7 | 9 | 261 | 10 | 261 | 6.54e-50 | 166 |
| MsG0380014744.01.T01 | MTR_5g031100 | 39.630 | 270 | 133 | 8 | 17 | 261 | 14 | 278 | 4.73e-49 | 164 |
| MsG0380014744.01.T01 | MTR_8g068030 | 37.956 | 274 | 131 | 9 | 9 | 261 | 10 | 265 | 6.48e-49 | 163 |
| MsG0380014744.01.T01 | MTR_5g031120 | 40.221 | 271 | 131 | 8 | 17 | 261 | 14 | 279 | 8.58e-49 | 163 |
| MsG0380014744.01.T01 | MTR_3g054170 | 40.530 | 264 | 144 | 5 | 1 | 261 | 1 | 254 | 1.16e-46 | 157 |
| MsG0380014744.01.T01 | MTR_5g031160 | 39.286 | 252 | 135 | 8 | 23 | 261 | 41 | 287 | 4.76e-46 | 156 |
| MsG0380014744.01.T01 | MTR_5g031140 | 38.489 | 278 | 142 | 9 | 7 | 261 | 15 | 286 | 7.51e-46 | 155 |
| MsG0380014744.01.T01 | MTR_3g047140 | 40.458 | 262 | 139 | 8 | 6 | 261 | 11 | 261 | 2.40e-44 | 152 |
| MsG0380014744.01.T01 | MTR_3g054180 | 40.755 | 265 | 144 | 5 | 1 | 261 | 5 | 260 | 9.93e-44 | 149 |
| MsG0380014744.01.T01 | MTR_3g027280 | 39.535 | 258 | 131 | 9 | 12 | 257 | 18 | 262 | 3.21e-43 | 149 |
| MsG0380014744.01.T01 | MTR_8g068050 | 38.806 | 268 | 142 | 7 | 10 | 264 | 18 | 276 | 3.25e-43 | 156 |
| MsG0380014744.01.T01 | MTR_5g031090 | 36.653 | 251 | 137 | 8 | 27 | 261 | 31 | 275 | 2.05e-41 | 144 |
| MsG0380014744.01.T01 | MTR_5g031030 | 36.400 | 250 | 135 | 8 | 27 | 261 | 34 | 274 | 4.99e-40 | 140 |
| MsG0380014744.01.T01 | MTR_8g067980 | 40.891 | 247 | 119 | 12 | 31 | 262 | 26 | 260 | 5.02e-39 | 137 |
| MsG0380014744.01.T01 | MTR_8g067940 | 36.194 | 268 | 153 | 9 | 10 | 261 | 16 | 281 | 4.29e-38 | 135 |
| MsG0380014744.01.T01 | MTR_1g090973 | 35.870 | 276 | 157 | 9 | 6 | 274 | 11 | 273 | 7.54e-38 | 135 |
| MsG0380014744.01.T01 | MTR_8g067930 | 37.838 | 259 | 138 | 8 | 16 | 261 | 11 | 259 | 3.54e-37 | 139 |
| MsG0380014744.01.T01 | MTR_0163s0020 | 36.531 | 271 | 152 | 6 | 7 | 271 | 14 | 270 | 6.65e-37 | 132 |
| MsG0380014744.01.T01 | MTR_0684s0020 | 36.531 | 271 | 152 | 6 | 7 | 271 | 13 | 269 | 7.53e-37 | 132 |
| MsG0380014744.01.T01 | MTR_1g090957 | 37.452 | 259 | 123 | 10 | 12 | 257 | 18 | 250 | 1.33e-36 | 131 |
| MsG0380014744.01.T01 | MTR_4g093080 | 35.897 | 273 | 151 | 9 | 16 | 276 | 14 | 274 | 4.65e-36 | 136 |
| MsG0380014744.01.T01 | MTR_8g067690 | 36.667 | 240 | 134 | 7 | 32 | 261 | 63 | 294 | 3.20e-35 | 134 |
| MsG0380014744.01.T01 | MTR_8g067720 | 32.103 | 271 | 160 | 7 | 1 | 261 | 1 | 257 | 9.93e-35 | 132 |
| MsG0380014744.01.T01 | MTR_8g067630 | 39.189 | 222 | 114 | 8 | 32 | 242 | 33 | 244 | 4.82e-34 | 130 |
| MsG0380014744.01.T01 | MTR_3g054200 | 35.985 | 264 | 156 | 5 | 1 | 261 | 1 | 254 | 2.47e-33 | 122 |
| MsG0380014744.01.T01 | MTR_8g067990 | 38.683 | 243 | 124 | 11 | 31 | 259 | 26 | 257 | 3.37e-33 | 122 |
| MsG0380014744.01.T01 | MTR_4g093140 | 33.206 | 262 | 152 | 9 | 12 | 263 | 18 | 266 | 9.86e-33 | 127 |
| MsG0380014744.01.T01 | MTR_8g067960 | 37.131 | 237 | 126 | 9 | 37 | 261 | 16 | 241 | 1.58e-32 | 120 |
| MsG0380014744.01.T01 | MTR_8g067735 | 33.577 | 274 | 154 | 9 | 1 | 261 | 20 | 278 | 1.98e-31 | 123 |
| MsG0380014744.01.T01 | MTR_1g090930 | 37.402 | 254 | 129 | 9 | 16 | 256 | 22 | 258 | 7.96e-31 | 117 |
| MsG0380014744.01.T01 | MTR_1g090943 | 35.125 | 279 | 152 | 11 | 9 | 274 | 15 | 277 | 9.10e-31 | 116 |
| MsG0380014744.01.T01 | MTR_1g090937 | 35.060 | 251 | 137 | 8 | 15 | 256 | 21 | 254 | 2.75e-30 | 115 |
| MsG0380014744.01.T01 | MTR_1g014240 | 33.191 | 235 | 129 | 7 | 43 | 261 | 53 | 275 | 9.09e-29 | 115 |
| MsG0380014744.01.T01 | MTR_7g116130 | 38.333 | 240 | 112 | 12 | 43 | 262 | 39 | 262 | 9.56e-29 | 115 |
| MsG0380014744.01.T01 | MTR_4g093110 | 31.559 | 263 | 155 | 10 | 12 | 263 | 18 | 266 | 5.22e-28 | 113 |
| MsG0380014744.01.T01 | MTR_4g093040 | 34.818 | 247 | 132 | 12 | 21 | 250 | 25 | 259 | 9.35e-28 | 112 |
| MsG0380014744.01.T01 | MTR_4g093040 | 38.710 | 186 | 93 | 9 | 77 | 250 | 1 | 177 | 6.83e-26 | 107 |
| MsG0380014744.01.T01 | MTR_4g093070 | 32.589 | 224 | 117 | 6 | 58 | 263 | 61 | 268 | 4.75e-25 | 104 |
| MsG0380014744.01.T01 | MTR_7g115740 | 32.971 | 276 | 152 | 13 | 18 | 275 | 14 | 274 | 1.22e-24 | 103 |
| MsG0380014744.01.T01 | MTR_1g090960 | 31.429 | 280 | 134 | 9 | 6 | 274 | 11 | 243 | 5.92e-24 | 97.4 |
| MsG0380014744.01.T01 | MTR_7g062940 | 29.924 | 264 | 156 | 7 | 12 | 257 | 3 | 255 | 1.37e-20 | 91.7 |
| MsG0380014744.01.T01 | MTR_1g048360 | 32.353 | 204 | 114 | 7 | 66 | 259 | 71 | 260 | 2.94e-20 | 90.9 |
| MsG0380014744.01.T01 | MTR_5g025020 | 30.734 | 218 | 123 | 8 | 66 | 267 | 85 | 290 | 8.04e-18 | 83.6 |
| MsG0380014744.01.T01 | MTR_7g062890 | 29.588 | 267 | 150 | 8 | 14 | 257 | 5 | 256 | 2.99e-17 | 82.0 |
| MsG0380014744.01.T01 | MTR_7g062950 | 28.302 | 265 | 159 | 8 | 12 | 257 | 3 | 255 | 8.60e-17 | 80.5 |
| MsG0380014744.01.T01 | MTR_7g063030 | 28.044 | 271 | 165 | 8 | 4 | 257 | 3 | 260 | 1.32e-16 | 80.1 |
| MsG0380014744.01.T01 | MTR_5g068770 | 31.776 | 214 | 116 | 9 | 64 | 259 | 76 | 277 | 3.10e-16 | 79.0 |
| MsG0380014744.01.T01 | MTR_5g035910 | 29.779 | 272 | 154 | 13 | 8 | 257 | 7 | 263 | 4.02e-16 | 78.6 |
| MsG0380014744.01.T01 | MTR_7g062700 | 29.927 | 274 | 151 | 9 | 8 | 257 | 2 | 258 | 5.08e-16 | 78.2 |
| MsG0380014744.01.T01 | MTR_5g077100 | 29.358 | 218 | 132 | 5 | 69 | 279 | 68 | 270 | 8.79e-16 | 77.4 |
| MsG0380014744.01.T01 | MTR_4g093050 | 33.010 | 206 | 109 | 9 | 67 | 257 | 71 | 262 | 1.44e-15 | 77.0 |
| MsG0380014744.01.T01 | MTR_4g056320 | 28.517 | 263 | 134 | 7 | 6 | 261 | 14 | 229 | 2.08e-15 | 74.7 |
| MsG0380014744.01.T01 | MTR_7g063030 | 29.741 | 232 | 137 | 7 | 43 | 257 | 32 | 254 | 2.55e-15 | 76.3 |
| MsG0380014744.01.T01 | MTR_1g052275 | 29.323 | 266 | 158 | 8 | 12 | 257 | 3 | 258 | 2.99e-15 | 75.9 |
| MsG0380014744.01.T01 | MTR_8g068050 | 29.891 | 184 | 115 | 4 | 10 | 186 | 18 | 194 | 4.87e-15 | 75.1 |
| MsG0380014744.01.T01 | MTR_7g062750 | 28.195 | 266 | 161 | 7 | 12 | 257 | 3 | 258 | 6.43e-15 | 75.1 |
| MsG0380014744.01.T01 | MTR_7g063010 | 27.203 | 261 | 159 | 8 | 14 | 257 | 10 | 256 | 6.46e-15 | 75.1 |
| MsG0380014744.01.T01 | MTR_7g062990 | 26.515 | 264 | 164 | 7 | 14 | 257 | 6 | 259 | 1.18e-13 | 71.2 |
| MsG0380014744.01.T01 | MTR_7g062770 | 29.536 | 237 | 135 | 6 | 43 | 257 | 34 | 260 | 4.55e-13 | 69.3 |
| MsG0380014744.01.T01 | MTR_7g062680 | 26.692 | 266 | 166 | 7 | 12 | 257 | 3 | 259 | 9.76e-13 | 68.6 |
| MsG0380014744.01.T01 | MTR_7g062900 | 26.695 | 236 | 134 | 7 | 43 | 257 | 34 | 251 | 1.60e-11 | 64.7 |
| MsG0380014744.01.T01 | MTR_7g115740 | 31.792 | 173 | 99 | 9 | 18 | 180 | 14 | 177 | 5.36e-11 | 63.2 |
| MsG0380014744.01.T01 | MTR_7g062920 | 28.727 | 275 | 153 | 10 | 8 | 257 | 2 | 258 | 6.96e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014744.01.T01 | AT5G10530 | 34.221 | 263 | 153 | 8 | 11 | 263 | 4 | 256 | 3.38e-29 | 116 |
| MsG0380014744.01.T01 | AT5G65600 | 33.478 | 230 | 137 | 6 | 32 | 250 | 38 | 262 | 6.12e-27 | 110 |
| MsG0380014744.01.T01 | AT4G02420 | 31.679 | 262 | 148 | 10 | 14 | 257 | 12 | 260 | 2.16e-20 | 91.3 |
| MsG0380014744.01.T01 | AT2G37710 | 29.661 | 236 | 133 | 7 | 43 | 257 | 38 | 261 | 8.65e-20 | 89.4 |
| MsG0380014744.01.T01 | AT2G29250 | 29.457 | 258 | 155 | 7 | 17 | 257 | 14 | 261 | 1.44e-19 | 88.6 |
| MsG0380014744.01.T01 | AT3G16530 | 27.957 | 279 | 155 | 11 | 14 | 259 | 6 | 271 | 5.59e-19 | 84.7 |
| MsG0380014744.01.T01 | AT4G02410 | 30.830 | 253 | 150 | 7 | 25 | 260 | 22 | 266 | 7.17e-19 | 86.7 |
| MsG0380014744.01.T01 | AT5G60280 | 31.518 | 257 | 143 | 9 | 19 | 257 | 15 | 256 | 1.85e-18 | 85.5 |
| MsG0380014744.01.T01 | AT5G01560 | 29.688 | 256 | 141 | 10 | 32 | 259 | 30 | 274 | 1.98e-18 | 85.5 |
| MsG0380014744.01.T01 | AT3G53810 | 28.458 | 253 | 152 | 8 | 24 | 257 | 19 | 261 | 3.48e-18 | 84.7 |
| MsG0380014744.01.T01 | AT5G01540 | 29.183 | 257 | 148 | 9 | 29 | 259 | 30 | 278 | 4.94e-18 | 84.3 |
| MsG0380014744.01.T01 | AT4G29050 | 31.381 | 239 | 138 | 7 | 48 | 269 | 53 | 282 | 8.35e-18 | 83.6 |
| MsG0380014744.01.T01 | AT4G29050 | 31.381 | 239 | 138 | 7 | 48 | 269 | 39 | 268 | 1.15e-17 | 83.2 |
| MsG0380014744.01.T01 | AT3G55550 | 30.041 | 243 | 145 | 6 | 32 | 258 | 26 | 259 | 1.25e-17 | 83.2 |
| MsG0380014744.01.T01 | AT5G01550 | 27.208 | 283 | 167 | 9 | 9 | 259 | 15 | 290 | 1.39e-17 | 82.8 |
| MsG0380014744.01.T01 | AT3G15356 | 29.365 | 252 | 130 | 10 | 43 | 258 | 31 | 270 | 3.10e-17 | 79.7 |
| MsG0380014744.01.T01 | AT3G45430 | 31.179 | 263 | 135 | 12 | 26 | 263 | 22 | 263 | 3.79e-17 | 81.6 |
| MsG0380014744.01.T01 | AT3G45430 | 31.179 | 263 | 135 | 12 | 26 | 263 | 22 | 263 | 3.79e-17 | 81.6 |
| MsG0380014744.01.T01 | AT3G45430 | 31.179 | 263 | 135 | 12 | 26 | 263 | 22 | 263 | 3.79e-17 | 81.6 |
| MsG0380014744.01.T01 | AT2G29220 | 27.715 | 267 | 164 | 8 | 10 | 258 | 7 | 262 | 4.98e-16 | 78.2 |
| MsG0380014744.01.T01 | AT5G55830 | 28.782 | 271 | 156 | 12 | 6 | 257 | 12 | 264 | 1.47e-15 | 77.0 |
| MsG0380014744.01.T01 | AT5G60300 | 31.496 | 254 | 132 | 13 | 28 | 257 | 23 | 258 | 2.19e-15 | 76.6 |
| MsG0380014744.01.T01 | AT5G60300 | 31.496 | 254 | 132 | 13 | 28 | 257 | 23 | 258 | 2.19e-15 | 76.6 |
| MsG0380014744.01.T01 | AT5G60300 | 31.496 | 254 | 132 | 13 | 28 | 257 | 23 | 258 | 2.65e-15 | 76.3 |
| MsG0380014744.01.T01 | AT3G08870 | 25.681 | 257 | 162 | 8 | 26 | 259 | 30 | 280 | 1.34e-14 | 73.9 |
| MsG0380014744.01.T01 | AT3G08870 | 25.681 | 257 | 162 | 8 | 26 | 259 | 51 | 301 | 1.39e-14 | 73.9 |
| MsG0380014744.01.T01 | AT5G03140 | 29.358 | 218 | 133 | 7 | 48 | 255 | 51 | 257 | 1.67e-14 | 73.9 |
| MsG0380014744.01.T01 | AT5G06740 | 31.220 | 205 | 118 | 9 | 64 | 258 | 78 | 269 | 7.84e-14 | 71.6 |
| MsG0380014744.01.T01 | AT5G06740 | 31.220 | 205 | 118 | 9 | 64 | 258 | 66 | 257 | 8.23e-14 | 71.6 |
| MsG0380014744.01.T01 | AT3G45440 | 29.457 | 258 | 152 | 10 | 17 | 257 | 65 | 309 | 2.57e-13 | 70.5 |
| MsG0380014744.01.T01 | AT1G07460 | 29.098 | 244 | 131 | 9 | 41 | 260 | 38 | 263 | 2.99e-13 | 69.3 |
| MsG0380014744.01.T01 | AT3G45440 | 29.457 | 258 | 152 | 10 | 17 | 257 | 12 | 256 | 3.13e-13 | 70.1 |
| MsG0380014744.01.T01 | AT3G45440 | 29.457 | 258 | 152 | 10 | 17 | 257 | 12 | 256 | 3.13e-13 | 70.1 |
| MsG0380014744.01.T01 | AT3G45440 | 29.457 | 258 | 152 | 10 | 17 | 257 | 12 | 256 | 3.13e-13 | 70.1 |
| MsG0380014744.01.T01 | AT5G42120 | 30.453 | 243 | 135 | 9 | 36 | 257 | 27 | 256 | 1.04e-12 | 68.6 |
| MsG0380014744.01.T01 | AT1G70110 | 27.386 | 241 | 140 | 11 | 43 | 262 | 33 | 259 | 1.13e-12 | 68.2 |
| MsG0380014744.01.T01 | AT3G53380 | 32.685 | 257 | 149 | 12 | 10 | 255 | 1 | 244 | 2.00e-12 | 67.8 |
| MsG0380014744.01.T01 | AT5G03350 | 28.369 | 282 | 148 | 14 | 14 | 259 | 6 | 269 | 4.48e-12 | 65.5 |
| MsG0380014744.01.T01 | AT3G45410 | 26.493 | 268 | 167 | 10 | 10 | 260 | 5 | 259 | 4.80e-12 | 66.6 |
| MsG0380014744.01.T01 | AT4G04960 | 28.440 | 218 | 124 | 6 | 58 | 255 | 48 | 253 | 4.02e-11 | 63.5 |
| MsG0380014744.01.T01 | AT4G04960 | 28.440 | 218 | 124 | 6 | 58 | 255 | 48 | 253 | 5.17e-11 | 63.2 |
Find 68 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTGTGCTACTTATATTTC+CGG | 0.221234 | 3:-59243960 | None:intergenic |
| CTACTTATATTTCCGGGATT+TGG | 0.229840 | 3:-59243953 | None:intergenic |
| ATTCTGCCCCTATTCACATT+TGG | 0.267516 | 3:+59243696 | MsG0380014744.01.T01:CDS |
| CTTGGAAGATGAGATTCTTT+TGG | 0.279704 | 3:-59243598 | None:intergenic |
| CTTCCCTGTTGAGGTAGTTT+CGG | 0.316382 | 3:-59243627 | None:intergenic |
| TTCTGCCCCTATTCACATTT+GGG | 0.317850 | 3:+59243697 | MsG0380014744.01.T01:CDS |
| AATCACTGTTCCGTGGAGTT+TGG | 0.318353 | 3:+59244021 | MsG0380014744.01.T01:CDS |
| CCTCCTTGACGAATTGATTT+CGG | 0.320012 | 3:-59243839 | None:intergenic |
| AATATCATGTGCTGCATATT+CGG | 0.332848 | 3:-59244212 | None:intergenic |
| TGCTATCCCAAATGTGAATA+GGG | 0.336758 | 3:-59243703 | None:intergenic |
| TTGCTATCCCAAATGTGAAT+AGG | 0.349217 | 3:-59243704 | None:intergenic |
| AATGTTGTGATAGGGTTTAA+TGG | 0.356600 | 3:+59244061 | MsG0380014744.01.T01:CDS |
| GTGGGTAAGGATTGGTTTCT+CGG | 0.358994 | 3:+59244177 | MsG0380014744.01.T01:CDS |
| GAGTTTGGAAAATAATAAAA+AGG | 0.376135 | 3:+59244036 | MsG0380014744.01.T01:CDS |
| AGTAGCACAGGTCGACATAT+TGG | 0.377327 | 3:+59243971 | MsG0380014744.01.T01:CDS |
| CCTGTGCTACTTATATTTCC+GGG | 0.385267 | 3:-59243959 | None:intergenic |
| CACATTTGGGATAGCAAAAC+CGG | 0.385321 | 3:+59243710 | MsG0380014744.01.T01:CDS |
| CACTTTCTATAATGCTCAAT+GGG | 0.387120 | 3:+59243928 | MsG0380014744.01.T01:CDS |
| AAGTCATGTTGTGCCTTTGA+AGG | 0.401051 | 3:+59244141 | MsG0380014744.01.T01:CDS |
| TTCAAAGTTAGCCAAGCTTC+CGG | 0.402294 | 3:-59243729 | None:intergenic |
| GATGCCGAAACTACCTCAAC+AGG | 0.405476 | 3:+59243623 | MsG0380014744.01.T01:CDS |
| GGATAGGATAAAAGAGAACT+TGG | 0.414580 | 3:-59243489 | None:intergenic |
| ATTGGTTTCTCGGCTGCGAC+TGG | 0.417093 | 3:+59244187 | MsG0380014744.01.T01:CDS |
| TACCTTCACCTTTACAATAA+CGG | 0.427007 | 3:+59243754 | MsG0380014744.01.T01:CDS |
| TCAAAGGCACAACATGACTT+AGG | 0.457672 | 3:-59244138 | None:intergenic |
| TAGCAAGATGAAGAATGTTA+AGG | 0.459173 | 3:-59243516 | None:intergenic |
| AAGGCAGTCAAGAACTCTAT+TGG | 0.460512 | 3:+59243662 | MsG0380014744.01.T01:CDS |
| ACACTTTCTATAATGCTCAA+TGG | 0.461585 | 3:+59243927 | MsG0380014744.01.T01:CDS |
| GTTCCTGAGTGGGTAAGGAT+TGG | 0.467906 | 3:+59244169 | MsG0380014744.01.T01:CDS |
| AAGTTGTTCCTGAGTGGGTA+AGG | 0.471213 | 3:+59244164 | MsG0380014744.01.T01:CDS |
| TCTGCAACATTATAAGTATT+AGG | 0.482296 | 3:-59243779 | None:intergenic |
| ATCAACACTCAACACATTTG+TGG | 0.492912 | 3:-59244086 | None:intergenic |
| GATGAAGAATGTTAAGGAGA+TGG | 0.494586 | 3:-59243510 | None:intergenic |
| TCCATCACAAAGTTTGTCCC+AGG | 0.494662 | 3:+59243575 | MsG0380014744.01.T01:CDS |
| GACAAACTTTGTGATGGAAA+AGG | 0.501076 | 3:-59243570 | None:intergenic |
| GGATAGCAAAACCGGAAGCT+TGG | 0.501666 | 3:+59243718 | MsG0380014744.01.T01:CDS |
| CTTCATCTTGCTATTGAACA+AGG | 0.506685 | 3:+59243526 | MsG0380014744.01.T01:CDS |
| GCTCAATGGGATCCAAATCC+CGG | 0.507888 | 3:+59243941 | MsG0380014744.01.T01:CDS |
| TTGAACAGAGCTTGAAACAT+TGG | 0.512201 | 3:-59244287 | None:intergenic |
| ATTAGGCGCCGTTATTGTAA+AGG | 0.518797 | 3:-59243762 | None:intergenic |
| CATTGATGTTATCAAAACCT+AGG | 0.523343 | 3:-59244264 | None:intergenic |
| AAAAGGCTAATGTTGTGATA+GGG | 0.526554 | 3:+59244053 | MsG0380014744.01.T01:CDS |
| CAAAGGCACAACATGACTTA+GGG | 0.534314 | 3:-59244137 | None:intergenic |
| GAAGGAAGTTGTTCCTGAGT+GGG | 0.537596 | 3:+59244159 | MsG0380014744.01.T01:CDS |
| GCCTGGGACAAACTTTGTGA+TGG | 0.542810 | 3:-59243576 | None:intergenic |
| TTTGATTCAAAGTTGAACCT+AGG | 0.546087 | 3:+59244247 | MsG0380014744.01.T01:CDS |
| TAGGATAAAAGAGAACTTGG+AGG | 0.547182 | 3:-59243486 | None:intergenic |
| CCCGGAAATATAAGTAGCAC+AGG | 0.548535 | 3:+59243959 | MsG0380014744.01.T01:CDS |
| TCAGGAACAACTTCCTTCAA+AGG | 0.548600 | 3:-59244154 | None:intergenic |
| CGCCGTTATTGTAAAGGTGA+AGG | 0.549680 | 3:-59243756 | None:intergenic |
| GAGTTCTAACTTCCCTGTTG+AGG | 0.551336 | 3:-59243636 | None:intergenic |
| GATTTCGGCTGAGTATCAAT+CGG | 0.563335 | 3:-59243824 | None:intergenic |
| AGAATGTTAAGGAGATGGAT+AGG | 0.563697 | 3:-59243505 | None:intergenic |
| ATTCGTCAAGGAGGATACCT+TGG | 0.568952 | 3:+59243848 | MsG0380014744.01.T01:CDS |
| CAAAAGAATCTCATCTTCCA+AGG | 0.575599 | 3:+59243599 | MsG0380014744.01.T01:CDS |
| AGGGTATAATGACGAATCAA+AGG | 0.576589 | 3:-59244118 | None:intergenic |
| AAACCAATCCTTACCCACTC+AGG | 0.577788 | 3:-59244172 | None:intergenic |
| TTCTGCTATTGAAAACTCCA+AGG | 0.589730 | 3:-59243865 | None:intergenic |
| TGAAGGAAGTTGTTCCTGAG+TGG | 0.603890 | 3:+59244158 | MsG0380014744.01.T01:CDS |
| AATCAATAATCACTGTTCCG+TGG | 0.606891 | 3:+59244014 | MsG0380014744.01.T01:CDS |
| GCTATCCCAAATGTGAATAG+GGG | 0.608736 | 3:-59243702 | None:intergenic |
| AAATGTGTTGAGTGTTGATG+TGG | 0.622877 | 3:+59244090 | MsG0380014744.01.T01:CDS |
| AGGTAGTTTCGGCATCACCT+TGG | 0.636564 | 3:-59243616 | None:intergenic |
| AGGGAAGTTAGAACTCAGCA+AGG | 0.647782 | 3:+59243643 | MsG0380014744.01.T01:CDS |
| AATACTTATAATGTTGCAGA+CGG | 0.651921 | 3:+59243782 | MsG0380014744.01.T01:CDS |
| CAGCCGAAATCAATTCGTCA+AGG | 0.655408 | 3:+59243836 | MsG0380014744.01.T01:CDS |
| CCGAAATCAATTCGTCAAGG+AGG | 0.688967 | 3:+59243839 | MsG0380014744.01.T01:CDS |
| ATGCCGAAACTACCTCAACA+GGG | 0.739522 | 3:+59243624 | MsG0380014744.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTTTGGAAAATAATAAAA+AGG | + | Chr3:59244036-59244055 | MsG0380014744.01.T01:CDS | 20.0% |
| ! | AATACTTATAATGTTGCAGA+CGG | + | Chr3:59243782-59243801 | MsG0380014744.01.T01:CDS | 25.0% |
| ! | TCTGCAACATTATAAGTATT+AGG | - | Chr3:59243782-59243801 | None:intergenic | 25.0% |
| !! | TTATTATTTTCCAAACTCCA+CGG | - | Chr3:59244034-59244053 | None:intergenic | 25.0% |
| !!! | CAACAGTTTGAATTGATTTT+TGG | - | Chr3:59243895-59243914 | None:intergenic | 25.0% |
| AAAAAGGCTAATGTTGTGAT+AGG | + | Chr3:59244052-59244071 | MsG0380014744.01.T01:CDS | 30.0% | |
| AAAAGGCTAATGTTGTGATA+GGG | + | Chr3:59244053-59244072 | MsG0380014744.01.T01:CDS | 30.0% | |
| AATATCATGTGCTGCATATT+CGG | - | Chr3:59244215-59244234 | None:intergenic | 30.0% | |
| AATGTTGTGATAGGGTTTAA+TGG | + | Chr3:59244061-59244080 | MsG0380014744.01.T01:CDS | 30.0% | |
| ACACTTTCTATAATGCTCAA+TGG | + | Chr3:59243927-59243946 | MsG0380014744.01.T01:CDS | 30.0% | |
| CACTTTCTATAATGCTCAAT+GGG | + | Chr3:59243928-59243947 | MsG0380014744.01.T01:CDS | 30.0% | |
| CATTGATGTTATCAAAACCT+AGG | - | Chr3:59244267-59244286 | None:intergenic | 30.0% | |
| TACCTTCACCTTTACAATAA+CGG | + | Chr3:59243754-59243773 | MsG0380014744.01.T01:CDS | 30.0% | |
| TAGCAAGATGAAGAATGTTA+AGG | - | Chr3:59243519-59243538 | None:intergenic | 30.0% | |
| TTTGATTCAAAGTTGAACCT+AGG | + | Chr3:59244247-59244266 | MsG0380014744.01.T01:CDS | 30.0% | |
| AATCAATAATCACTGTTCCG+TGG | + | Chr3:59244014-59244033 | MsG0380014744.01.T01:CDS | 35.0% | |
| AGAATGTTAAGGAGATGGAT+AGG | - | Chr3:59243508-59243527 | None:intergenic | 35.0% | |
| AGGGTATAATGACGAATCAA+AGG | - | Chr3:59244121-59244140 | None:intergenic | 35.0% | |
| ATCAACACTCAACACATTTG+TGG | - | Chr3:59244089-59244108 | None:intergenic | 35.0% | |
| CAAAAGAATCTCATCTTCCA+AGG | + | Chr3:59243599-59243618 | MsG0380014744.01.T01:CDS | 35.0% | |
| CTACTTATATTTCCGGGATT+TGG | - | Chr3:59243956-59243975 | None:intergenic | 35.0% | |
| GATGAAGAATGTTAAGGAGA+TGG | - | Chr3:59243513-59243532 | None:intergenic | 35.0% | |
| GGATAGGATAAAAGAGAACT+TGG | - | Chr3:59243492-59243511 | None:intergenic | 35.0% | |
| TAGGATAAAAGAGAACTTGG+AGG | - | Chr3:59243489-59243508 | None:intergenic | 35.0% | |
| TGCTATCCCAAATGTGAATA+GGG | - | Chr3:59243706-59243725 | None:intergenic | 35.0% | |
| TTCTGCTATTGAAAACTCCA+AGG | - | Chr3:59243868-59243887 | None:intergenic | 35.0% | |
| TTGAACAGAGCTTGAAACAT+TGG | - | Chr3:59244290-59244309 | None:intergenic | 35.0% | |
| TTGCTATCCCAAATGTGAAT+AGG | - | Chr3:59243707-59243726 | None:intergenic | 35.0% | |
| ! | CAGCACATGATATTTTATCG+TGG | + | Chr3:59244221-59244240 | MsG0380014744.01.T01:CDS | 35.0% |
| ! | CTTGGAAGATGAGATTCTTT+TGG | - | Chr3:59243601-59243620 | None:intergenic | 35.0% |
| ! | GACAAACTTTGTGATGGAAA+AGG | - | Chr3:59243573-59243592 | None:intergenic | 35.0% |
| !! | AAATGTGTTGAGTGTTGATG+TGG | + | Chr3:59244090-59244109 | MsG0380014744.01.T01:CDS | 35.0% |
| !! | ACCTGTGCTACTTATATTTC+CGG | - | Chr3:59243963-59243982 | None:intergenic | 35.0% |
| !! | CTTCATCTTGCTATTGAACA+AGG | + | Chr3:59243526-59243545 | MsG0380014744.01.T01:CDS | 35.0% |
| AAGGCAGTCAAGAACTCTAT+TGG | + | Chr3:59243662-59243681 | MsG0380014744.01.T01:CDS | 40.0% | |
| AAGTCATGTTGTGCCTTTGA+AGG | + | Chr3:59244141-59244160 | MsG0380014744.01.T01:CDS | 40.0% | |
| ATTCTGCCCCTATTCACATT+TGG | + | Chr3:59243696-59243715 | MsG0380014744.01.T01:CDS | 40.0% | |
| GATTTCGGCTGAGTATCAAT+CGG | - | Chr3:59243827-59243846 | None:intergenic | 40.0% | |
| GCTATCCCAAATGTGAATAG+GGG | - | Chr3:59243705-59243724 | None:intergenic | 40.0% | |
| TCAGGAACAACTTCCTTCAA+AGG | - | Chr3:59244157-59244176 | None:intergenic | 40.0% | |
| TTCAAAGTTAGCCAAGCTTC+CGG | - | Chr3:59243732-59243751 | None:intergenic | 40.0% | |
| TTCTGCCCCTATTCACATTT+GGG | + | Chr3:59243697-59243716 | MsG0380014744.01.T01:CDS | 40.0% | |
| ! | AAGATGAGATTCTTTTGGCC+TGG | - | Chr3:59243596-59243615 | None:intergenic | 40.0% |
| ! | AGATGAGATTCTTTTGGCCT+GGG | - | Chr3:59243595-59243614 | None:intergenic | 40.0% |
| ! | ATTAGGCGCCGTTATTGTAA+AGG | - | Chr3:59243765-59243784 | None:intergenic | 40.0% |
| ! | CAAAGGCACAACATGACTTA+GGG | - | Chr3:59244140-59244159 | None:intergenic | 40.0% |
| ! | CACATTTGGGATAGCAAAAC+CGG | + | Chr3:59243710-59243729 | MsG0380014744.01.T01:CDS | 40.0% |
| ! | CCTCCTTGACGAATTGATTT+CGG | - | Chr3:59243842-59243861 | None:intergenic | 40.0% |
| ! | TCAAAGGCACAACATGACTT+AGG | - | Chr3:59244141-59244160 | None:intergenic | 40.0% |
| !! | CCTGTGCTACTTATATTTCC+GGG | - | Chr3:59243962-59243981 | None:intergenic | 40.0% |
| AAACCAATCCTTACCCACTC+AGG | - | Chr3:59244175-59244194 | None:intergenic | 45.0% | |
| AAGTTGTTCCTGAGTGGGTA+AGG | + | Chr3:59244164-59244183 | MsG0380014744.01.T01:CDS | 45.0% | |
| AATCACTGTTCCGTGGAGTT+TGG | + | Chr3:59244021-59244040 | MsG0380014744.01.T01:CDS | 45.0% | |
| AGGGAAGTTAGAACTCAGCA+AGG | + | Chr3:59243643-59243662 | MsG0380014744.01.T01:CDS | 45.0% | |
| AGTAGCACAGGTCGACATAT+TGG | + | Chr3:59243971-59243990 | MsG0380014744.01.T01:CDS | 45.0% | |
| ATGCCGAAACTACCTCAACA+GGG | + | Chr3:59243624-59243643 | MsG0380014744.01.T01:CDS | 45.0% | |
| ATTCGTCAAGGAGGATACCT+TGG | + | Chr3:59243848-59243867 | MsG0380014744.01.T01:CDS | 45.0% | |
| CAGCCGAAATCAATTCGTCA+AGG | + | Chr3:59243836-59243855 | MsG0380014744.01.T01:CDS | 45.0% | |
| CCCGGAAATATAAGTAGCAC+AGG | + | Chr3:59243959-59243978 | MsG0380014744.01.T01:CDS | 45.0% | |
| CCGAAATCAATTCGTCAAGG+AGG | + | Chr3:59243839-59243858 | MsG0380014744.01.T01:CDS | 45.0% | |
| CGCCGTTATTGTAAAGGTGA+AGG | - | Chr3:59243759-59243778 | None:intergenic | 45.0% | |
| CTTCCCTGTTGAGGTAGTTT+CGG | - | Chr3:59243630-59243649 | None:intergenic | 45.0% | |
| GAAGGAAGTTGTTCCTGAGT+GGG | + | Chr3:59244159-59244178 | MsG0380014744.01.T01:CDS | 45.0% | |
| TCCATCACAAAGTTTGTCCC+AGG | + | Chr3:59243575-59243594 | MsG0380014744.01.T01:CDS | 45.0% | |
| TGAAGGAAGTTGTTCCTGAG+TGG | + | Chr3:59244158-59244177 | MsG0380014744.01.T01:CDS | 45.0% | |
| ! | GAGTTCTAACTTCCCTGTTG+AGG | - | Chr3:59243639-59243658 | None:intergenic | 45.0% |
| !! | GTGGGTAAGGATTGGTTTCT+CGG | + | Chr3:59244177-59244196 | MsG0380014744.01.T01:CDS | 45.0% |
| AGGTAGTTTCGGCATCACCT+TGG | - | Chr3:59243619-59243638 | None:intergenic | 50.0% | |
| GATGCCGAAACTACCTCAAC+AGG | + | Chr3:59243623-59243642 | MsG0380014744.01.T01:CDS | 50.0% | |
| GCCTGGGACAAACTTTGTGA+TGG | - | Chr3:59243579-59243598 | None:intergenic | 50.0% | |
| GCTCAATGGGATCCAAATCC+CGG | + | Chr3:59243941-59243960 | MsG0380014744.01.T01:CDS | 50.0% | |
| GGATAGCAAAACCGGAAGCT+TGG | + | Chr3:59243718-59243737 | MsG0380014744.01.T01:CDS | 50.0% | |
| GTTCCTGAGTGGGTAAGGAT+TGG | + | Chr3:59244169-59244188 | MsG0380014744.01.T01:CDS | 50.0% | |
| ! | ATTGGTTTCTCGGCTGCGAC+TGG | + | Chr3:59244187-59244206 | MsG0380014744.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 59243479 | 59244318 | 59243479 | ID=MsG0380014744.01;Name=MsG0380014744.01 |
| Chr3 | mRNA | 59243479 | 59244318 | 59243479 | ID=MsG0380014744.01.T01;Parent=MsG0380014744.01;Name=MsG0380014744.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|279 |
| Chr3 | exon | 59243479 | 59244318 | 59243479 | ID=MsG0380014744.01.T01:exon:11532;Parent=MsG0380014744.01.T01 |
| Chr3 | CDS | 59243479 | 59244318 | 59243479 | ID=MsG0380014744.01.T01:cds;Parent=MsG0380014744.01.T01 |
| Gene Sequence |
| Protein sequence |
