AlfalfaGEDB | MsG0580026810.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026810.01

KEGG: K15028
GO: GO:0003743 GO:0005488 GO:0005852 GO:0006446 GO:0043022
PFAM: PF10075

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026810.01.T01	XP_003609944.1	94.22	173	5	1	20	192	1	168	1.48E-112	331

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026810.01.T01	Q94HF1	79.487	156	32	0	37	192	5	160	2.28E-89	265

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026810.01.T01	G7JS43	94.220	173	5	1	20	192	1	168	7.05e-113	331

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048809.01	MsG0580026810.01	0.842473	2.530497e-58	3.841416e-55
MsG0180003118.01	MsG0580026810.01	0.802292	6.013136e-49	2.934653e-46
MsG0180004599.01	MsG0580026810.01	-0.802812	4.696282e-49	2.322678e-46
MsG0180005354.01	MsG0580026810.01	-0.810448	1.146964e-50	6.915381e-48
MsG0280010976.01	MsG0580026810.01	-0.806841	6.762778e-50	3.708858e-47
MsG0380012279.01	MsG0580026810.01	0.826263	2.986081e-54	2.786426e-51
MsG0380014551.01	MsG0580026810.01	0.812922	3.322894e-51	2.139743e-48
MsG0380014866.01	MsG0580026810.01	-0.807340	5.303352e-50	2.946361e-47
MsG0480023937.01	MsG0580026810.01	-0.810393	1.178997e-50	7.097963e-48
MsG0380017401.01	MsG0580026810.01	-0.821832	3.273425e-53	2.692981e-50
MsG0480018192.01	MsG0580026810.01	0.820288	7.424047e-53	5.849812e-50
MsG0580026810.01	MsG0580028664.01	0.808978	2.374198e-50	1.376896e-47
MsG0580026810.01	MsG0680031377.01	0.818502	1.895573e-52	1.421417e-49
MsG0580026810.01	MsG0680034914.01	-0.805806	1.117618e-49	5.968991e-47
MsG0580026810.01	MsG0880042743.01	-0.801347	9.398280e-49	4.478021e-46
MsG0580026810.01	MsG0880046561.01	-0.808547	2.934913e-50	1.682930e-47
MsG0580026810.01	MsG0880047594.01	-0.811265	7.631955e-51	4.702674e-48
MsG0280006935.01	MsG0580026810.01	-0.822434	2.373743e-53	1.986716e-50
MsG0280007864.01	MsG0580026810.01	0.801972	6.996840e-49	3.386720e-46

PPI

Gene1	Gene2	Type
MsG0780036859.01	MsG0580026810.01	PPI
MsG0780040280.01	MsG0580026810.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026810.01.T01	MTR_4g124640	94.220	173	5	1	20	192	1	168	1.79e-116	331

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026810.01.T01	AT4G33250	79.874	159	30	1	34	192	6	162	2.66e-90	264

Find 40 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATCATCACATTGAAAATT+AGG	0.220004	5:-45293144	None:intergenic
CTGGAAGGTTTCGTCAGTTC+TGG	0.234923	5:+45293550	MsG0580026810.01.T01:CDS
CACATTGAAAATTAGGAATA+TGG	0.260139	5:-45293137	None:intergenic
ACCTCAGCAAGCACAGTTCT+AGG	0.298434	5:-45293678	None:intergenic
TCGAGATCTTGAAGAATATC+AGG	0.305382	5:-45293285	None:intergenic
GCACAGTTCTAGGAATCTTT+TGG	0.326432	5:-45293668	None:intergenic
TCCTCCATTTGCACACGTTC+CGG	0.335385	5:-45293486	None:intergenic
CCCAACCAGAGTTGGCTACT+TGG	0.386040	5:-45293727	None:intergenic
TGATAACTTGATTTCGATCA+TGG	0.396588	5:+45293161	MsG0580026810.01.T01:CDS
TGGATTGCCTGCTCAAATCC+TGG	0.411523	5:-45293612	None:intergenic
CGTTGCTCGCATATTGGTTA+AGG	0.419052	5:+45293413	MsG0580026810.01.T01:CDS
CGAAAATTATGTCAATGATC+AGG	0.427681	5:+45293305	MsG0580026810.01.T01:CDS
CATATAGTTGAAGCCGTGCC+AGG	0.433378	5:+45293594	MsG0580026810.01.T01:CDS
TGGAAGGTTTCGTCAGTTCT+GGG	0.441956	5:+45293551	MsG0580026810.01.T01:CDS
GATTCCGGAACGTGTGCAAA+TGG	0.456092	5:+45293482	MsG0580026810.01.T01:CDS
CACAGGCTGAAGTGTGGAGC+TGG	0.466087	5:-45293450	None:intergenic
TGCTTGCTGAGGTTTATCCT+TGG	0.466350	5:+45293688	MsG0580026810.01.T01:CDS
TCAGATCGTTGCTCGCATAT+TGG	0.484728	5:+45293407	MsG0580026810.01.T01:CDS
CTGTCTCACTATTTAGAGAC+TGG	0.488200	5:+45293531	MsG0580026810.01.T01:CDS
TCCTTGCTGTTGTACTTGTA+CGG	0.492803	5:-45293208	None:intergenic
TCTCAGGTTCAAACTGGTAG+AGG	0.496339	5:-45293374	None:intergenic
GAACATGAATCGCATAGCCT+TGG	0.508528	5:-45293632	None:intergenic
ACAAGTACAACAGCAAGGAC+AGG	0.520513	5:+45293212	MsG0580026810.01.T01:CDS
CAAACTGGTAGAGGCGAAGA+AGG	0.520837	5:-45293365	None:intergenic
CTCACTATTTAGAGACTGGA+AGG	0.523087	5:+45293535	MsG0580026810.01.T01:CDS
CATATTGGTTAAGGCCCTCA+TGG	0.529992	5:+45293422	MsG0580026810.01.T01:CDS
AGCCGTGCCAGGATTTGAGC+AGG	0.543997	5:+45293605	MsG0580026810.01.T01:CDS
GAAGGCATAGATTTGATTCG+AGG	0.557097	5:-45293347	None:intergenic
TCCTAGAACTGTGCTTGCTG+AGG	0.561352	5:+45293677	MsG0580026810.01.T01:CDS
GGATTTGAGCAGGCAATCCA+AGG	0.567214	5:+45293615	MsG0580026810.01.T01:CDS
TGGAGCTGGCATAGCCATGA+GGG	0.572588	5:-45293436	None:intergenic
GATAACTTGATTTCGATCAT+GGG	0.598344	5:+45293162	MsG0580026810.01.T01:CDS
CAAGTACAACAGCAAGGACA+GGG	0.623697	5:+45293213	MsG0580026810.01.T01:CDS
TGCCTGCTCAAATCCTGGCA+CGG	0.624671	5:-45293607	None:intergenic
AAGAGACACAGGCTGAAGTG+TGG	0.626839	5:-45293456	None:intergenic
TCCGGAACGTGTGCAAATGG+AGG	0.627671	5:+45293485	MsG0580026810.01.T01:CDS
AAGCTGTTCGACAGTGTAAG+CGG	0.629108	5:-45293241	None:intergenic
GTGGAGCTGGCATAGCCATG+AGG	0.671134	5:-45293437	None:intergenic
GCCGTACAAGTACAACAGCA+AGG	0.674180	5:+45293207	MsG0580026810.01.T01:CDS
GTACAACAGCAAGGACAGGG+AGG	0.722492	5:+45293216	MsG0580026810.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATCATCACATTGAAAATT+AGG	-	Chr5:45293147-45293166	None:intergenic	20.0%
!	CACATTGAAAATTAGGAATA+TGG	-	Chr5:45293140-45293159	None:intergenic	25.0%
	CGAAAATTATGTCAATGATC+AGG	+	Chr5:45293305-45293324	MsG0580026810.01.T01:CDS	30.0%
	GATAACTTGATTTCGATCAT+GGG	+	Chr5:45293162-45293181	MsG0580026810.01.T01:CDS	30.0%
	TGATAACTTGATTTCGATCA+TGG	+	Chr5:45293161-45293180	MsG0580026810.01.T01:CDS	30.0%
	TCGAGATCTTGAAGAATATC+AGG	-	Chr5:45293288-45293307	None:intergenic	35.0%
!	AGGAATCTTTTGGTAAGTCA+AGG	-	Chr5:45293661-45293680	None:intergenic	35.0%
!	ATCTGAGAACTCATTTTCTC+AGG	-	Chr5:45293393-45293412	None:intergenic	35.0%
!	TCATTTTCTCAGGTTCAAAC+TGG	-	Chr5:45293383-45293402	None:intergenic	35.0%
!!	GTTTTCAAGGAATTTGTCCA+AGG	-	Chr5:45293708-45293727	None:intergenic	35.0%
	CTCACTATTTAGAGACTGGA+AGG	+	Chr5:45293535-45293554	MsG0580026810.01.T01:CDS	40.0%
	CTGTCTCACTATTTAGAGAC+TGG	+	Chr5:45293531-45293550	MsG0580026810.01.T01:CDS	40.0%
!	GAAGGCATAGATTTGATTCG+AGG	-	Chr5:45293350-45293369	None:intergenic	40.0%
!!	CCTGTGTCTCTTTTTGATTC+CGG	+	Chr5:45293467-45293486	MsG0580026810.01.T01:CDS	40.0%
!!	TCCTTGCTGTTGTACTTGTA+CGG	-	Chr5:45293211-45293230	None:intergenic	40.0%
!!!	GCACAGTTCTAGGAATCTTT+TGG	-	Chr5:45293671-45293690	None:intergenic	40.0%
	AAGCTGTTCGACAGTGTAAG+CGG	-	Chr5:45293244-45293263	None:intergenic	45.0%
	ACAAGTACAACAGCAAGGAC+AGG	+	Chr5:45293212-45293231	MsG0580026810.01.T01:CDS	45.0%
	CAAGTACAACAGCAAGGACA+GGG	+	Chr5:45293213-45293232	MsG0580026810.01.T01:CDS	45.0%
	CCGGAATCAAAAAGAGACAC+AGG	-	Chr5:45293470-45293489	None:intergenic	45.0%
	GAACATGAATCGCATAGCCT+TGG	-	Chr5:45293635-45293654	None:intergenic	45.0%
	TCAGATCGTTGCTCGCATAT+TGG	+	Chr5:45293407-45293426	MsG0580026810.01.T01:CDS	45.0%
	TCTCAGGTTCAAACTGGTAG+AGG	-	Chr5:45293377-45293396	None:intergenic	45.0%
	TGGAAGGTTTCGTCAGTTCT+GGG	+	Chr5:45293551-45293570	MsG0580026810.01.T01:CDS	45.0%
!	CATATTGGTTAAGGCCCTCA+TGG	+	Chr5:45293422-45293441	MsG0580026810.01.T01:CDS	45.0%
!	CGTTGCTCGCATATTGGTTA+AGG	+	Chr5:45293413-45293432	MsG0580026810.01.T01:CDS	45.0%
!	TGCTTGCTGAGGTTTATCCT+TGG	+	Chr5:45293688-45293707	MsG0580026810.01.T01:CDS	45.0%
	AAGAGACACAGGCTGAAGTG+TGG	-	Chr5:45293459-45293478	None:intergenic	50.0%
	ACCTCAGCAAGCACAGTTCT+AGG	-	Chr5:45293681-45293700	None:intergenic	50.0%
	CAAACTGGTAGAGGCGAAGA+AGG	-	Chr5:45293368-45293387	None:intergenic	50.0%
	CATATAGTTGAAGCCGTGCC+AGG	+	Chr5:45293594-45293613	MsG0580026810.01.T01:CDS	50.0%
	CTGGAAGGTTTCGTCAGTTC+TGG	+	Chr5:45293550-45293569	MsG0580026810.01.T01:CDS	50.0%
	GATTCCGGAACGTGTGCAAA+TGG	+	Chr5:45293482-45293501	MsG0580026810.01.T01:CDS	50.0%
	GCCGTACAAGTACAACAGCA+AGG	+	Chr5:45293207-45293226	MsG0580026810.01.T01:CDS	50.0%
	GGATTTGAGCAGGCAATCCA+AGG	+	Chr5:45293615-45293634	MsG0580026810.01.T01:CDS	50.0%
	TCCTCCATTTGCACACGTTC+CGG	-	Chr5:45293489-45293508	None:intergenic	50.0%
	TGGATTGCCTGCTCAAATCC+TGG	-	Chr5:45293615-45293634	None:intergenic	50.0%
!	TCCTAGAACTGTGCTTGCTG+AGG	+	Chr5:45293677-45293696	MsG0580026810.01.T01:CDS	50.0%
	GTACAACAGCAAGGACAGGG+AGG	+	Chr5:45293216-45293235	MsG0580026810.01.T01:CDS	55.0%
	TCCGGAACGTGTGCAAATGG+AGG	+	Chr5:45293485-45293504	MsG0580026810.01.T01:CDS	55.0%
	TGCCTGCTCAAATCCTGGCA+CGG	-	Chr5:45293610-45293629	None:intergenic	55.0%
	TGGAGCTGGCATAGCCATGA+GGG	-	Chr5:45293439-45293458	None:intergenic	55.0%
	AGCCGTGCCAGGATTTGAGC+AGG	+	Chr5:45293605-45293624	MsG0580026810.01.T01:CDS	60.0%
	CACAGGCTGAAGTGTGGAGC+TGG	-	Chr5:45293453-45293472	None:intergenic	60.0%
	GTGGAGCTGGCATAGCCATG+AGG	-	Chr5:45293440-45293459	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	45293123	45293734	45293123	ID=MsG0580026810.01;Name=MsG0580026810.01
Chr5	mRNA	45293123	45293734	45293123	ID=MsG0580026810.01.T01;Parent=MsG0580026810.01;Name=MsG0580026810.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|203
Chr5	exon	45293123	45293734	45293123	ID=MsG0580026810.01.T01:exon:13946;Parent=MsG0580026810.01.T01
Chr5	CDS	45293123	45293734	45293123	ID=MsG0580026810.01.T01:cds;Parent=MsG0580026810.01.T01

Gene Sequence

>MsG0580026810.01.T01

ATGTTGCAAAACATCCATATTCCTAATTTTCAATGTGATGATAACTTGATTTCGATCATGGGAAGAGAAGCAGCAGCAGCAGCAGCCGTACAAGTACAACAGCAAGGACAGGGAGGAACCGCTTACACTGTCGAACAGCTTGTTGCATTCAATCGTTACAATCCTGATATTCTTCAAGATCTCGAAAATTATGTCAATGATCAGGTTTCATCGCAAACTTACAGCCTCGAATCAAATCTATGCCTTCTTCGCCTCTACCAGTTTGAACCTGAGAAAATGAGTTCTCAGATCGTTGCTCGCATATTGGTTAAGGCCCTCATGGCTATGCCAGCTCCACACTTCAGCCTGTGTCTCTTTTTGATTCCGGAACGTGTGCAAATGGAGGAGCAGTTCAAGACTCTGATTGTTCTGTCTCACTATTTAGAGACTGGAAGGTTTCGTCAGTTCTGGGATGAAGCATCGAAAAGTCGTCATATAGTTGAAGCCGTGCCAGGATTTGAGCAGGCAATCCAAGGCTATGCGATTCATGTTCTATCCTTGACTTACCAAAAGATTCCTAGAACTGTGCTTGCTGAGGTTTATCCTTGGACAAATTCCTTGAAAACCAAGTAG

Protein sequence

>MsG0580026810.01.T01

MLQNIHIPNFQCDDNLISIMGREAAAAAAVQVQQQGQGGTAYTVEQLVAFNRYNPDILQDLENYVNDQVSSQTYSLESNLCLLRLYQFEPEKMSSQIVARILVKALMAMPAPHFSLCLFLIPERVQMEEQFKTLIVLSHYLETGRFRQFWDEASKSRHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEVYPWTNSLKTK*