AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046561.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046561.01.T01 MTR_4g110100 73.585 106 6 2 1 84 317 422 1.02e-43 146
MsG0880046561.01.T01 MTR_7g074170 35.238 105 46 2 2 84 303 407 1.48e-14 67.4
MsG0880046561.01.T01 MTR_6g016350 39.048 105 42 1 1 83 268 372 5.75e-14 65.5
MsG0880046561.01.T01 MTR_6g079660 37.113 97 39 1 2 76 193 289 1.77e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046561.01.T01 AT5G08139 43.434 99 34 2 1 77 266 364 4.84e-18 77.0
MsG0880046561.01.T01 AT3G02340 36.364 99 41 1 2 78 293 391 2.52e-16 72.4
MsG0880046561.01.T01 AT3G02340 36.364 99 41 1 2 78 293 391 2.52e-16 72.4
MsG0880046561.01.T01 AT5G15820 34.694 98 42 1 2 77 249 346 3.26e-14 66.2
MsG0880046561.01.T01 AT5G01980 37.255 102 41 3 2 81 309 409 8.68e-13 62.4

Find 21 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGAAGCCGGTGGCTTAC+TGG 0.359157 8:-74915987 None:intergenic
AAGTGCTCCGAGCCAACATT+GGG 0.417324 8:-74916594 None:intergenic
GGAAACTCACAGCGACAAAC+AGG 0.463610 8:-74916538 None:intergenic
CAAGTGCTCCGAGCCAACAT+TGG 0.495300 8:-74916595 None:intergenic
TTCGTTTACCAGTAAGCCAC+CGG 0.508581 8:+74915979 MsG0880046561.01.T01:CDS
GCGGAGTATGAGATGATGTT+TGG 0.518188 8:+74915935 None:intergenic
TTGTAAGATGAATTTGCTGT+TGG 0.543992 8:+74916084 MsG0880046561.01.T01:CDS
GATGATGTTTGGTCAATTCG+CGG 0.544306 8:+74915946 None:intergenic
TCAACAAACGCTACAGAAGC+CGG 0.552415 8:-74915998 None:intergenic
TGTTGAGAATCTTCCGTCGG+TGG 0.555357 8:+74916015 MsG0880046561.01.T01:CDS
TGAATTTGCTGTTGGAGAAG+AGG 0.578557 8:+74916092 MsG0880046561.01.T01:CDS
GGAAAGCCCCAATGTTGGCT+CGG 0.582098 8:+74916587 MsG0880046561.01.T01:CDS
TCATAATCAGCATCATCAGT+AGG 0.603013 8:-74916559 None:intergenic
GGTTGTGACTAAAGAGGACG+TGG 0.612162 8:+74916036 MsG0880046561.01.T01:CDS
GTCGGTGGTTGTGACTAAAG+AGG 0.623252 8:+74916030 MsG0880046561.01.T01:CDS
TCTTTAGTCACAACCACCGA+CGG 0.649000 8:-74916028 None:intergenic
GTTTGTTGAGAATCTTCCGT+CGG 0.653060 8:+74916012 MsG0880046561.01.T01:CDS
ACGTAGGAAAGCCCCAATGT+TGG 0.654719 8:+74916582 MsG0880046561.01.T01:CDS
AGTGCTCCGAGCCAACATTG+GGG 0.718946 8:-74916593 None:intergenic
ATGATGCTGATTATGAACGT+AGG 0.726355 8:+74916566 MsG0880046561.01.T01:CDS
ACAAACGCTACAGAAGCCGG+TGG 0.728119 8:-74915995 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGCAGTTTGTAAAGATGAAT+TGG + Chr8:74916429-74916448 MsG0880046561.01.T01:intron 30.0%
! TGATTGTATTATGCCTTGTT+GGG + Chr8:74916499-74916518 MsG0880046561.01.T01:intron 30.0%
!! TTGTAAGATGAATTTGCTGT+TGG + Chr8:74916084-74916103 MsG0880046561.01.T01:CDS 30.0%
ATGGTGATTGTATTATGCCT+TGG + Chr8:74916145-74916164 MsG0880046561.01.T01:intron 35.0%
ATTGTATTATGCCTTGGTTG+GGG + Chr8:74916151-74916170 MsG0880046561.01.T01:intron 35.0%
CACATGATATCGATGAGAAA+CGG - Chr8:74916481-74916500 None:intergenic 35.0%
GAACTTCACCTGATAAGTTT+AGG - Chr8:74916213-74916232 None:intergenic 35.0%
GATTGTATTATGCCTTGGTT+GGG + Chr8:74916150-74916169 MsG0880046561.01.T01:intron 35.0%
GTGAGTTTCCTAAACTTATC+AGG + Chr8:74916202-74916221 MsG0880046561.01.T01:intron 35.0%
GTGATTGTATTATGCCTTGT+TGG + Chr8:74916498-74916517 MsG0880046561.01.T01:intron 35.0%
TCATAATCAGCATCATCAGT+AGG - Chr8:74916562-74916581 None:intergenic 35.0%
TGATTGTATTATGCCTTGGT+TGG + Chr8:74916149-74916168 MsG0880046561.01.T01:intron 35.0%
TGTAAAGATGAATTGGCTGT+TGG + Chr8:74916436-74916455 MsG0880046561.01.T01:intron 35.0%
! ATGATGCTGATTATGAACGT+AGG + Chr8:74916566-74916585 MsG0880046561.01.T01:CDS 35.0%
! GATTGTATTATGCCTTGTTG+GGG + Chr8:74916500-74916519 MsG0880046561.01.T01:intron 35.0%
AGTATTCCTAATCCCCAACA+AGG - Chr8:74916515-74916534 None:intergenic 40.0%
ATGATGACAAGAAGACACTG+CGG + Chr8:74916257-74916276 MsG0880046561.01.T01:intron 40.0%
ATTCGAGAACGTTTACCAGT+CGG + Chr8:74916360-74916379 MsG0880046561.01.T01:intron 40.0%
CATCATTGTCCCCAAAAATC+CGG - Chr8:74916240-74916259 None:intergenic 40.0%
CCATGATATCGATGAGAACA+CGG - Chr8:74916129-74916148 None:intergenic 40.0%
GTTGTTATCCAGTCCTCTTA+CGG - Chr8:74916398-74916417 None:intergenic 40.0%
! AAGTTCAGAGCCGGATTTTT+GGG + Chr8:74916227-74916246 MsG0880046561.01.T01:intron 40.0%
! ATTATGCCTTGTTGGGGATT+AGG + Chr8:74916506-74916525 MsG0880046561.01.T01:intron 40.0%
! GTTTGTTGAGAATCTTCCGT+CGG + Chr8:74916012-74916031 MsG0880046561.01.T01:CDS 40.0%
!! TGAATTTGCTGTTGGAGAAG+AGG + Chr8:74916092-74916111 MsG0880046561.01.T01:CDS 40.0%
ATTGACAAACATCTCAGCCG+CGG - Chr8:74916342-74916361 None:intergenic 45.0%
CCGTGTTCTCATCGATATCA+TGG + Chr8:74916126-74916145 MsG0880046561.01.T01:intron 45.0%
GTATTCCTAATCCCCAACCA+AGG - Chr8:74916165-74916184 None:intergenic 45.0%
TATCAGGTGAAGTTCAGAGC+CGG + Chr8:74916218-74916237 MsG0880046561.01.T01:intron 45.0%
TCAACAAACGCTACAGAAGC+CGG - Chr8:74916001-74916020 None:intergenic 45.0%
TCGTTTACAAGTAACCACCG+CGG + Chr8:74916322-74916341 MsG0880046561.01.T01:intron 45.0%
TCTTTAGTCACAACCACCGA+CGG - Chr8:74916031-74916050 None:intergenic 45.0%
TGAATTGGCTGTTGGAGAAG+AGG + Chr8:74916444-74916463 MsG0880046561.01.T01:intron 45.0%
TTATGCCTTGGTTGGGGATT+AGG + Chr8:74916157-74916176 MsG0880046561.01.T01:intron 45.0%
TTCGAGAACGTTTACCAGTC+GGG + Chr8:74916361-74916380 MsG0880046561.01.T01:intron 45.0%
TTCGTTTACCAGTAAGCCAC+CGG + Chr8:74915979-74915998 MsG0880046561.01.T01:CDS 45.0%
! AGTTCAGAGCCGGATTTTTG+GGG + Chr8:74916228-74916247 MsG0880046561.01.T01:intron 45.0%
! GAAGTTCAGAGCCGGATTTT+TGG + Chr8:74916226-74916245 MsG0880046561.01.T01:intron 45.0%
AAGTGCTCCGAGCCAACATT+GGG - Chr8:74916597-74916616 None:intergenic 50.0%
ACAAGAAGACACTGCGGAGT+AGG + Chr8:74916263-74916282 MsG0880046561.01.T01:intron 50.0%
ACGTAGGAAAGCCCCAATGT+TGG + Chr8:74916582-74916601 MsG0880046561.01.T01:CDS 50.0%
GGAAACTCACAGCGACAAAC+AGG - Chr8:74916192-74916211 None:intergenic 50.0%
GGTTGTGACTAAAGAGGACG+TGG + Chr8:74916036-74916055 MsG0880046561.01.T01:CDS 50.0%
GTCGGTGGTTGTGACTAAAG+AGG + Chr8:74916030-74916049 MsG0880046561.01.T01:CDS 50.0%
TCGAGAACGTTTACCAGTCG+GGG + Chr8:74916362-74916381 MsG0880046561.01.T01:intron 50.0%
TGTTGAGAATCTTCCGTCGG+TGG + Chr8:74916015-74916034 MsG0880046561.01.T01:CDS 50.0%
!! ATGATGGTGGGTCACAATCG+CGG + Chr8:74916288-74916307 MsG0880046561.01.T01:intron 50.0%
ACAAACGCTACAGAAGCCGG+TGG - Chr8:74915998-74916017 None:intergenic 55.0%
ACGTTTACCAGTCGGGGTTG+TGG + Chr8:74916368-74916387 MsG0880046561.01.T01:intron 55.0%
AGTGCTCCGAGCCAACATTG+GGG - Chr8:74916596-74916615 None:intergenic 55.0%
CAAGTGCTCCGAGCCAACAT+TGG - Chr8:74916598-74916617 None:intergenic 55.0%
CACTGCGGAGTAGGAGATGA+TGG + Chr8:74916272-74916291 MsG0880046561.01.T01:intron 55.0%
GGGTTGTGGACTTCCGTAAG+AGG + Chr8:74916382-74916401 MsG0880046561.01.T01:intron 55.0%
GTGGACTTCCGTAAGAGGAC+TGG + Chr8:74916387-74916406 MsG0880046561.01.T01:intron 55.0%
TACAGAAGCCGGTGGCTTAC+TGG - Chr8:74915990-74916009 None:intergenic 55.0%
TGGCTGTTGGAGAAGAGGTG+AGG + Chr8:74916449-74916468 MsG0880046561.01.T01:intron 55.0%
! GCGGAGTAGGAGATGATGGT+GGG + Chr8:74916276-74916295 MsG0880046561.01.T01:intron 55.0%
! GGAAAGCCCCAATGTTGGCT+CGG + Chr8:74916587-74916606 MsG0880046561.01.T01:CDS 55.0%
! TGCGGAGTAGGAGATGATGG+TGG + Chr8:74916275-74916294 MsG0880046561.01.T01:intron 55.0%
GACAAACATCTCAGCCGCGG+TGG - Chr8:74916339-74916358 None:intergenic 60.0%
CGGAAGTCCACAACCCCGAC+TGG - Chr8:74916378-74916397 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 74915947 74916618 74915947 ID=MsG0880046561.01;Name=MsG0880046561.01
Chr8 mRNA 74915947 74916618 74915947 ID=MsG0880046561.01.T01;Parent=MsG0880046561.01;Name=MsG0880046561.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|85
Chr8 exon 74915947 74916113 74915947 ID=MsG0880046561.01.T01:exon:31359;Parent=MsG0880046561.01.T01
Chr8 exon 74916528 74916618 74916528 ID=MsG0880046561.01.T01:exon:31360;Parent=MsG0880046561.01.T01
Chr8 CDS 74915947 74916113 74915947 ID=MsG0880046561.01.T01:cds;Parent=MsG0880046561.01.T01
Chr8 CDS 74916528 74916618 74916528 ID=MsG0880046561.01.T01:cds;Parent=MsG0880046561.01.T01
Gene Sequence

>MsG0880046561.01.T01

ATGATGTTTGGTCAATTCGCGGATAATGTGAATTCGTTTACCAGTAAGCCACCGGCTTCTGTAGCGTTTGTTGAGAATCTTCCGTCGGTGGTTGTGACTAAAGAGGACGTGGATAACAACAATGCGTTGTGTGCAGTTTGTAAGATGAATTTGCTGTTGGAGAAGAGGAATACTTGTCCTGTTTGTCGCTGTGAGTTTCCTACTGATGATGCTGATTATGAACGTAGGAAAGCCCCAATGTTGGCTCGGAGCACTTGA

Protein sequence

>MsG0880046561.01.T01

MMFGQFADNVNSFTSKPPASVAFVENLPSVVVTKEDVDNNNALCAVCKMNLLLEKRNTCPVCRCEFPTDDADYERRKAPMLARST*