Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030843.01.T01 | ABW22423.1 | 99.51 | 204 | 1 | 0 | 1 | 204 | 1 | 204 | 1.73E-146 | 416 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030843.01.T01 | P26863 | 33.654 | 208 | 81 | 6 | 5 | 203 | 3 | 162 | 1.49E-22 | 95.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030843.01.T01 | A8IFN5 | 99.510 | 204 | 1 | 0 | 1 | 204 | 1 | 204 | 8.27e-147 | 416 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048809.01 | MsG0680030843.01 | 0.828852 | 7.138438e-55 | 7.180619e-52 |
| MsG0180000240.01 | MsG0680030843.01 | 0.837860 | 4.050801e-57 | 5.330991e-54 |
| MsG0180003309.01 | MsG0680030843.01 | 0.810799 | 9.629445e-51 | 5.860758e-48 |
| MsG0180005881.01 | MsG0680030843.01 | 0.827156 | 1.827894e-54 | 1.750163e-51 |
| MsG0280006346.01 | MsG0680030843.01 | 0.805205 | 1.493802e-49 | 7.855828e-47 |
| MsG0280011419.01 | MsG0680030843.01 | 0.812842 | 3.458590e-51 | 2.222397e-48 |
| MsG0380012279.01 | MsG0680030843.01 | 0.844825 | 5.947749e-59 | 9.720143e-56 |
| MsG0380014551.01 | MsG0680030843.01 | 0.807269 | 5.489072e-50 | 3.043990e-47 |
| MsG0380014770.01 | MsG0680030843.01 | -0.800445 | 1.436250e-48 | 6.688792e-46 |
| MsG0680030843.01 | MsG0680031377.01 | 0.836347 | 9.867543e-57 | 1.240257e-53 |
| MsG0680030843.01 | MsG0680034909.01 | 0.805187 | 1.506553e-49 | 7.919198e-47 |
| MsG0680030843.01 | MsG0780039867.01 | 0.833860 | 4.183776e-56 | 4.879852e-53 |
| MsG0680030843.01 | MsG0780040576.01 | 0.826715 | 2.329840e-54 | 2.202205e-51 |
| MsG0680030843.01 | MsG0880044455.01 | 0.839291 | 1.730300e-57 | 2.378634e-54 |
| MsG0480022615.01 | MsG0680030843.01 | 0.872416 | 3.235046e-67 | 1.352708e-63 |
| MsG0580024363.01 | MsG0680030843.01 | 0.833481 | 5.203179e-56 | 5.999614e-53 |
| MsG0380017551.01 | MsG0680030843.01 | 0.834570 | 2.776375e-56 | 3.308151e-53 |
| MsG0580029999.01 | MsG0680030843.01 | 0.855321 | 6.822962e-62 | 1.569293e-58 |
| MsG0280007864.01 | MsG0680030843.01 | 0.854780 | 9.801845e-62 | 2.214088e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030843.01.T01 | MTR_6g012880 | 97.549 | 204 | 5 | 0 | 1 | 204 | 1 | 204 | 7.77e-148 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCTCCCACGAAGATTC+AGG | 0.267746 | 6:+10305547 | MsG0680030843.01.T01:CDS |
| AGCCACCAGGTTTGAGAATA+AGG | 0.278963 | 6:+10305359 | MsG0680030843.01.T01:CDS |
| TGAGAATAAGGTGGGATTCT+TGG | 0.283152 | 6:+10305371 | MsG0680030843.01.T01:CDS |
| ACACATCAGAAAGAAGAATT+TGG | 0.283979 | 6:+10305413 | MsG0680030843.01.T01:CDS |
| AGCTGCCAGGTTTAATGATA+TGG | 0.336529 | 6:+10305488 | MsG0680030843.01.T01:CDS |
| GGATGAAACTGAATAAGATT+TGG | 0.359256 | 6:+10305583 | MsG0680030843.01.T01:CDS |
| AACCTGGTGGCTGCGTTGAT+AGG | 0.409309 | 6:-10305348 | None:intergenic |
| CTTAGGGAAAGAAACGTTCT+TGG | 0.410244 | 6:+10305269 | MsG0680030843.01.T01:CDS |
| CTTTCTGATGTGTGTTTCAC+CGG | 0.412209 | 6:-10305403 | None:intergenic |
| TTCTTGGTGGATGCAGGACC+TGG | 0.415168 | 6:+10305285 | MsG0680030843.01.T01:CDS |
| CATTCCGTTCTCACAGCAAA+AGG | 0.450534 | 6:+10305694 | MsG0680030843.01.T01:CDS |
| TTCTTTGATCAGTGGTTCAC+CGG | 0.453887 | 6:-10305460 | None:intergenic |
| TTTGGGGTTAATGCTCTCAA+TGG | 0.466840 | 6:-10305748 | None:intergenic |
| GATTCAGGCAAGACCGAGCT+TGG | 0.473612 | 6:+10305562 | MsG0680030843.01.T01:CDS |
| CGGTCTTGCCTGAATCTTCG+TGG | 0.477095 | 6:-10305555 | None:intergenic |
| AATGAACTTGAATTACATCT+AGG | 0.479422 | 6:-10305207 | None:intergenic |
| TCAAGTTCATTCTTATGTAG+TGG | 0.480693 | 6:+10305219 | MsG0680030843.01.T01:CDS |
| TCAAAGAACGAGCAGCTGCC+AGG | 0.484964 | 6:+10305475 | MsG0680030843.01.T01:CDS |
| GAAACGTTCTTGGTGGATGC+AGG | 0.497487 | 6:+10305279 | MsG0680030843.01.T01:CDS |
| AGAGTATTGTGGTGTTCTAA+AGG | 0.502486 | 6:+10305775 | MsG0680030843.01.T01:CDS |
| GAGATTATTCATTGATCTAG+TGG | 0.529676 | 6:+10305437 | MsG0680030843.01.T01:CDS |
| GGATTCTTGGATCGAGCAGC+CGG | 0.536676 | 6:+10305384 | MsG0680030843.01.T01:CDS |
| GGTTATTCAGTAGCCATCGC+GGG | 0.537446 | 6:+10305654 | MsG0680030843.01.T01:CDS |
| TGCCTATCAACGCAGCCACC+AGG | 0.537533 | 6:+10305346 | MsG0680030843.01.T01:CDS |
| TATTCAGTTTCATCCAAGCT+CGG | 0.538379 | 6:-10305575 | None:intergenic |
| TTCATTCTTATGTAGTGGAA+AGG | 0.539863 | 6:+10305224 | MsG0680030843.01.T01:CDS |
| TTATTCATTGATCTAGTGGC+CGG | 0.553492 | 6:+10305441 | MsG0680030843.01.T01:CDS |
| GCTGCTCGTTCTTTGATCAG+TGG | 0.558817 | 6:-10305468 | None:intergenic |
| TTTATTATTGATAAAGTCAG+AGG | 0.559698 | 6:+10305630 | MsG0680030843.01.T01:CDS |
| GCCAGGTTTAATGATATGGC+AGG | 0.562991 | 6:+10305492 | MsG0680030843.01.T01:CDS |
| GCGCAAAACTTCAGATTGCA+AGG | 0.575047 | 6:-10305247 | None:intergenic |
| GGTCTTGCCTGAATCTTCGT+GGG | 0.575360 | 6:-10305554 | None:intergenic |
| ACCAGGTTTGAGAATAAGGT+GGG | 0.575722 | 6:+10305363 | MsG0680030843.01.T01:CDS |
| CTCACAGCAAAAGGCAAAGA+CGG | 0.575778 | 6:+10305703 | MsG0680030843.01.T01:CDS |
| TCCTGCCATATCATTAAACC+TGG | 0.578910 | 6:-10305493 | None:intergenic |
| AGGGAAAGAAACGTTCTTGG+TGG | 0.580645 | 6:+10305272 | MsG0680030843.01.T01:CDS |
| GGCAGGATCCACAGATGTAG+TGG | 0.581851 | 6:+10305509 | MsG0680030843.01.T01:CDS |
| TCCCACCTTATTCTCAAACC+TGG | 0.582226 | 6:-10305364 | None:intergenic |
| CGAACGAATACAAAGGTGAA+AGG | 0.595541 | 6:+10305606 | MsG0680030843.01.T01:CDS |
| ATTATTGATAAAGTCAGAGG+AGG | 0.597950 | 6:+10305633 | MsG0680030843.01.T01:CDS |
| TCTTGGTGGATGCAGGACCT+GGG | 0.605758 | 6:+10305286 | MsG0680030843.01.T01:CDS |
| TTCATCAGCCACTACATCTG+TGG | 0.609411 | 6:-10305517 | None:intergenic |
| CACCAGGTTTGAGAATAAGG+TGG | 0.623782 | 6:+10305362 | MsG0680030843.01.T01:CDS |
| CTTCGTGGGAGAAGAAGAAG+TGG | 0.639862 | 6:-10305540 | None:intergenic |
| CACCTTATTCTCAAACCTGG+TGG | 0.641320 | 6:-10305361 | None:intergenic |
| AGGTTATTCAGTAGCCATCG+CGG | 0.647116 | 6:+10305653 | MsG0680030843.01.T01:CDS |
| ACAGCAAAAGGCAAAGACGG+AGG | 0.694620 | 6:+10305706 | MsG0680030843.01.T01:CDS |
| ATAAGCAATGAAACCCGCGA+TGG | 0.706583 | 6:-10305667 | None:intergenic |
| GATTTGGCGAACGAATACAA+AGG | 0.774797 | 6:+10305599 | MsG0680030843.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATTATTGATAAAGTCAG+AGG | + | Chr6:10305630-10305649 | MsG0680030843.01.T01:CDS | 20.0% |
| ! | AATGAACTTGAATTACATCT+AGG | - | Chr6:10305210-10305229 | None:intergenic | 25.0% |
| !!! | ACCACAATACTCTTATTTTT+GGG | - | Chr6:10305768-10305787 | None:intergenic | 25.0% |
| ACACATCAGAAAGAAGAATT+TGG | + | Chr6:10305413-10305432 | MsG0680030843.01.T01:CDS | 30.0% | |
| ATTATTGATAAAGTCAGAGG+AGG | + | Chr6:10305633-10305652 | MsG0680030843.01.T01:CDS | 30.0% | |
| GAGATTATTCATTGATCTAG+TGG | + | Chr6:10305437-10305456 | MsG0680030843.01.T01:CDS | 30.0% | |
| GGATGAAACTGAATAAGATT+TGG | + | Chr6:10305583-10305602 | MsG0680030843.01.T01:CDS | 30.0% | |
| TCAAGTTCATTCTTATGTAG+TGG | + | Chr6:10305219-10305238 | MsG0680030843.01.T01:CDS | 30.0% | |
| ! | TTCATTCTTATGTAGTGGAA+AGG | + | Chr6:10305224-10305243 | MsG0680030843.01.T01:CDS | 30.0% |
| !!! | CACCACAATACTCTTATTTT+TGG | - | Chr6:10305769-10305788 | None:intergenic | 30.0% |
| !!! | CCACAATACTCTTATTTTTG+GGG | - | Chr6:10305767-10305786 | None:intergenic | 30.0% |
| TATTCAGTTTCATCCAAGCT+CGG | - | Chr6:10305578-10305597 | None:intergenic | 35.0% | |
| TTATTCATTGATCTAGTGGC+CGG | + | Chr6:10305441-10305460 | MsG0680030843.01.T01:CDS | 35.0% | |
| ! | TCATCTCGCGTACAATTTTT+GGG | - | Chr6:10305315-10305334 | None:intergenic | 35.0% |
| !! | AATCTGAAGTTTTGCGCTTA+GGG | + | Chr6:10305253-10305272 | MsG0680030843.01.T01:CDS | 35.0% |
| !! | CCCCAAAAATAAGAGTATTG+TGG | + | Chr6:10305764-10305783 | MsG0680030843.01.T01:CDS | 35.0% |
| ACCAGGTTTGAGAATAAGGT+GGG | + | Chr6:10305363-10305382 | MsG0680030843.01.T01:CDS | 40.0% | |
| AGCTGCCAGGTTTAATGATA+TGG | + | Chr6:10305488-10305507 | MsG0680030843.01.T01:CDS | 40.0% | |
| CGAACGAATACAAAGGTGAA+AGG | + | Chr6:10305606-10305625 | MsG0680030843.01.T01:CDS | 40.0% | |
| CTTAGGGAAAGAAACGTTCT+TGG | + | Chr6:10305269-10305288 | MsG0680030843.01.T01:CDS | 40.0% | |
| CTTTCTGATGTGTGTTTCAC+CGG | - | Chr6:10305406-10305425 | None:intergenic | 40.0% | |
| GATTTGGCGAACGAATACAA+AGG | + | Chr6:10305599-10305618 | MsG0680030843.01.T01:CDS | 40.0% | |
| TCCTGCCATATCATTAAACC+TGG | - | Chr6:10305496-10305515 | None:intergenic | 40.0% | |
| TGAGAATAAGGTGGGATTCT+TGG | + | Chr6:10305371-10305390 | MsG0680030843.01.T01:CDS | 40.0% | |
| TTCTTTGATCAGTGGTTCAC+CGG | - | Chr6:10305463-10305482 | None:intergenic | 40.0% | |
| ! | CATCTCGCGTACAATTTTTG+GGG | - | Chr6:10305314-10305333 | None:intergenic | 40.0% |
| ! | CTCATCTCGCGTACAATTTT+TGG | - | Chr6:10305316-10305335 | None:intergenic | 40.0% |
| ! | TTTGGGGTTAATGCTCTCAA+TGG | - | Chr6:10305751-10305770 | None:intergenic | 40.0% |
| !! | ATCTCGCGTACAATTTTTGG+GGG | - | Chr6:10305313-10305332 | None:intergenic | 40.0% |
| !! | CAATCTGAAGTTTTGCGCTT+AGG | + | Chr6:10305252-10305271 | MsG0680030843.01.T01:CDS | 40.0% |
| !! | TTTGCCTTTTGCTGTGAGAA+CGG | - | Chr6:10305701-10305720 | None:intergenic | 40.0% |
| AGCCACCAGGTTTGAGAATA+AGG | + | Chr6:10305359-10305378 | MsG0680030843.01.T01:CDS | 45.0% | |
| AGGGAAAGAAACGTTCTTGG+TGG | + | Chr6:10305272-10305291 | MsG0680030843.01.T01:CDS | 45.0% | |
| AGGTTATTCAGTAGCCATCG+CGG | + | Chr6:10305653-10305672 | MsG0680030843.01.T01:CDS | 45.0% | |
| ATAAGCAATGAAACCCGCGA+TGG | - | Chr6:10305670-10305689 | None:intergenic | 45.0% | |
| CACCAGGTTTGAGAATAAGG+TGG | + | Chr6:10305362-10305381 | MsG0680030843.01.T01:CDS | 45.0% | |
| CACCTTATTCTCAAACCTGG+TGG | - | Chr6:10305364-10305383 | None:intergenic | 45.0% | |
| CATTCCGTTCTCACAGCAAA+AGG | + | Chr6:10305694-10305713 | MsG0680030843.01.T01:CDS | 45.0% | |
| CTCACAGCAAAAGGCAAAGA+CGG | + | Chr6:10305703-10305722 | MsG0680030843.01.T01:CDS | 45.0% | |
| GCCAGGTTTAATGATATGGC+AGG | + | Chr6:10305492-10305511 | MsG0680030843.01.T01:CDS | 45.0% | |
| GCGCAAAACTTCAGATTGCA+AGG | - | Chr6:10305250-10305269 | None:intergenic | 45.0% | |
| TCCCACCTTATTCTCAAACC+TGG | - | Chr6:10305367-10305386 | None:intergenic | 45.0% | |
| TTCATCAGCCACTACATCTG+TGG | - | Chr6:10305520-10305539 | None:intergenic | 45.0% | |
| TTCTTCTCCCACGAAGATTC+AGG | + | Chr6:10305547-10305566 | MsG0680030843.01.T01:CDS | 45.0% | |
| !! | CTTTTGCTGTGAGAACGGAA+TGG | - | Chr6:10305696-10305715 | None:intergenic | 45.0% |
| ACAGCAAAAGGCAAAGACGG+AGG | + | Chr6:10305706-10305725 | MsG0680030843.01.T01:CDS | 50.0% | |
| CTTCGTGGGAGAAGAAGAAG+TGG | - | Chr6:10305543-10305562 | None:intergenic | 50.0% | |
| GCTGCTCGTTCTTTGATCAG+TGG | - | Chr6:10305471-10305490 | None:intergenic | 50.0% | |
| GGTCTTGCCTGAATCTTCGT+GGG | - | Chr6:10305557-10305576 | None:intergenic | 50.0% | |
| GGTTATTCAGTAGCCATCGC+GGG | + | Chr6:10305654-10305673 | MsG0680030843.01.T01:CDS | 50.0% | |
| ! | GAAACGTTCTTGGTGGATGC+AGG | + | Chr6:10305279-10305298 | MsG0680030843.01.T01:CDS | 50.0% |
| !! | GTACAATTTTTGGGGGTCCC+AGG | - | Chr6:10305306-10305325 | None:intergenic | 50.0% |
| CGGTCTTGCCTGAATCTTCG+TGG | - | Chr6:10305558-10305577 | None:intergenic | 55.0% | |
| GATTCAGGCAAGACCGAGCT+TGG | + | Chr6:10305562-10305581 | MsG0680030843.01.T01:CDS | 55.0% | |
| GGATTCTTGGATCGAGCAGC+CGG | + | Chr6:10305384-10305403 | MsG0680030843.01.T01:CDS | 55.0% | |
| GGCAGGATCCACAGATGTAG+TGG | + | Chr6:10305509-10305528 | MsG0680030843.01.T01:CDS | 55.0% | |
| TCAAAGAACGAGCAGCTGCC+AGG | + | Chr6:10305475-10305494 | MsG0680030843.01.T01:CDS | 55.0% | |
| ! | AACCTGGTGGCTGCGTTGAT+AGG | - | Chr6:10305351-10305370 | None:intergenic | 55.0% |
| ! | TCTTGGTGGATGCAGGACCT+GGG | + | Chr6:10305286-10305305 | MsG0680030843.01.T01:CDS | 55.0% |
| ! | TTCTTGGTGGATGCAGGACC+TGG | + | Chr6:10305285-10305304 | MsG0680030843.01.T01:CDS | 55.0% |
| TGCCTATCAACGCAGCCACC+AGG | + | Chr6:10305346-10305365 | MsG0680030843.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 10305180 | 10305794 | 10305180 | ID=MsG0680030843.01;Name=MsG0680030843.01 |
| Chr6 | mRNA | 10305180 | 10305794 | 10305180 | ID=MsG0680030843.01.T01;Parent=MsG0680030843.01;Name=MsG0680030843.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|204 |
| Chr6 | exon | 10305180 | 10305794 | 10305180 | ID=MsG0680030843.01.T01:exon:7932;Parent=MsG0680030843.01.T01 |
| Chr6 | CDS | 10305180 | 10305794 | 10305180 | ID=MsG0680030843.01.T01:cds;Parent=MsG0680030843.01.T01 |
| Gene Sequence |
| Protein sequence |
