AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041676.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041676.01.T02 MTR_7g115700 95.690 116 4 1 1 115 55 170 1.25e-80 234
MsG0780041676.01.T02 MTR_5g072510 71.296 108 25 3 1 104 64 169 4.81e-50 158
MsG0780041676.01.T02 MTR_2g016110 66.387 119 32 3 1 113 62 178 1.99e-47 150
MsG0780041676.01.T02 MTR_1g442860 70.707 99 24 2 2 97 93 189 1.55e-46 149
MsG0780041676.01.T02 MTR_4g094428 63.793 116 36 3 2 113 74 187 4.31e-46 149
MsG0780041676.01.T02 MTR_2g092950 64.912 114 34 3 2 111 51 162 3.78e-45 144
MsG0780041676.01.T02 MTR_4g079930 66.327 98 28 2 2 96 57 152 2.54e-44 142
MsG0780041676.01.T02 MTR_2g076400 67.347 98 27 2 2 96 61 156 1.69e-43 140
MsG0780041676.01.T02 MTR_1g080210 70.339 118 30 2 1 115 67 182 2.57e-43 140
MsG0780041676.01.T02 MTR_7g097030 72.115 104 24 2 2 102 92 193 8.35e-42 137
MsG0780041676.01.T02 MTR_1g069825 72.115 104 24 2 2 102 80 181 1.26e-41 137
MsG0780041676.01.T02 MTR_1g075990 71.154 104 25 2 2 102 81 182 5.75e-41 134
MsG0780041676.01.T02 MTR_3g031830 67.241 116 32 3 2 113 70 183 5.26e-40 132
MsG0780041676.01.T02 MTR_8g086350 69.388 98 25 2 2 96 59 154 1.29e-34 118
MsG0780041676.01.T01 MTR_7g115700 97.059 170 4 1 1 169 1 170 6.86e-123 343
MsG0780041676.01.T01 MTR_5g072510 72.263 137 31 4 26 158 36 169 1.09e-67 205
MsG0780041676.01.T01 MTR_1g442860 73.438 128 28 3 27 151 65 189 4.46e-64 196
MsG0780041676.01.T01 MTR_4g094428 63.758 149 42 4 25 164 44 189 7.30e-64 196
MsG0780041676.01.T01 MTR_2g016110 67.114 149 40 4 25 167 33 178 1.93e-63 193
MsG0780041676.01.T01 MTR_1g080210 73.288 146 33 3 27 169 40 182 1.94e-62 191
MsG0780041676.01.T01 MTR_2g092950 66.667 138 40 3 16 150 12 146 6.17e-62 189
MsG0780041676.01.T01 MTR_2g076400 68.217 129 35 3 25 150 31 156 5.40e-60 184
MsG0780041676.01.T01 MTR_1g075990 70.213 141 36 3 19 156 45 182 8.54e-60 184
MsG0780041676.01.T01 MTR_4g079930 66.667 129 37 3 25 150 27 152 1.80e-59 183
MsG0780041676.01.T01 MTR_7g097030 72.263 137 32 3 23 156 60 193 4.31e-59 183
MsG0780041676.01.T01 MTR_1g069825 73.684 133 29 3 27 156 52 181 1.57e-58 182
MsG0780041676.01.T01 MTR_3g031830 68.276 145 39 4 27 167 42 183 1.75e-56 177
MsG0780041676.01.T01 MTR_8g086350 68.462 130 33 4 25 150 29 154 3.78e-48 154
MsG0780041676.01.T01 MTR_1g114020 53.731 67 30 1 5 71 49 114 5.17e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041676.01.T02 AT5G58500 68.750 112 30 3 2 109 47 157 1.36e-49 155
MsG0780041676.01.T02 AT1G07090 63.393 112 36 2 2 110 59 168 2.32e-47 150
MsG0780041676.01.T02 AT2G42610 67.347 98 27 2 2 96 53 148 6.56e-44 141
MsG0780041676.01.T02 AT2G42610 67.347 98 27 2 2 96 53 148 6.56e-44 141
MsG0780041676.01.T02 AT5G28490 74.528 106 22 2 2 104 53 156 1.55e-43 140
MsG0780041676.01.T02 AT4G18610 66.667 96 26 2 5 96 68 161 7.84e-43 139
MsG0780041676.01.T02 AT3G04510 68.468 111 30 2 2 109 61 169 4.79e-42 137
MsG0780041676.01.T02 AT2G31160 73.585 106 22 3 2 103 82 185 2.19e-40 134
MsG0780041676.01.T02 AT1G16910 59.223 103 37 2 2 101 51 151 9.71e-39 127
MsG0780041676.01.T02 AT3G23290 75.510 98 19 2 2 96 76 171 2.24e-38 128
MsG0780041676.01.T02 AT1G78815 54.400 125 44 3 2 115 68 190 2.75e-37 125
MsG0780041676.01.T01 AT5G58500 70.423 142 36 4 26 163 18 157 1.84e-67 203
MsG0780041676.01.T01 AT1G07090 65.493 142 43 3 26 164 30 168 1.15e-64 197
MsG0780041676.01.T01 AT5G28490 76.296 135 26 3 27 158 25 156 8.99e-62 189
MsG0780041676.01.T01 AT3G04510 70.714 140 35 3 27 163 33 169 5.68e-60 185
MsG0780041676.01.T01 AT2G42610 67.969 128 35 3 26 150 24 148 2.85e-59 182
MsG0780041676.01.T01 AT2G42610 67.969 128 35 3 26 150 24 148 2.85e-59 182
MsG0780041676.01.T01 AT2G31160 75.556 135 26 4 27 157 54 185 3.91e-58 181
MsG0780041676.01.T01 AT4G18610 65.116 129 38 3 26 150 36 161 2.19e-57 178
MsG0780041676.01.T01 AT3G23290 75.573 131 26 3 23 150 44 171 2.73e-57 178
MsG0780041676.01.T01 AT1G78815 63.566 129 41 3 25 150 38 163 3.66e-55 172
MsG0780041676.01.T01 AT1G16910 58.955 134 49 3 25 155 21 151 3.35e-52 164

Find 49 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACTACCTTGATCAATT+TGG 0.137888 7:+93796931 MsG0780041676.01.T01:CDS
TGTTCCAATCTCTCCTCTTT+TGG 0.205312 7:-93796828 None:intergenic
AATCCAACTTGTCCTTTCTT+TGG 0.215866 7:+93796970 MsG0780041676.01.T01:CDS
ATCCAACTTGTCCTTTCTTT+GGG 0.324334 7:+93796971 MsG0780041676.01.T01:CDS
TTCACTACCTTGATCAATTT+GGG 0.326576 7:+93796932 MsG0780041676.01.T01:CDS
GTCGATGGTTTCTTAAATAC+TGG 0.330003 7:-93796858 None:intergenic
CATGGTGGAAGTAACCCTTT+TGG 0.395005 7:+93797096 MsG0780041676.01.T01:CDS
GCACATGGAGATGGAGGGTT+TGG 0.411549 7:-93796997 None:intergenic
AGAGCCAAAAGAGGAGAGAT+TGG 0.437889 7:+93796824 MsG0780041676.01.T01:CDS
CACTTCCATTGTAGCTGCTA+GGG 0.441671 7:-93796768 None:intergenic
TTGTCCTCTCCGTCAGGCGT+GGG 0.455000 7:+93797020 MsG0780041676.01.T01:CDS
ATGTGCTTGTCCTCTCCGTC+AGG 0.459665 7:+93797014 MsG0780041676.01.T01:CDS
TGATCAATTTGGGAAGACTA+AGG 0.465045 7:+93796942 MsG0780041676.01.T01:CDS
CTAACAGCACCGTCACCAAA+AGG 0.465453 7:-93797111 None:intergenic
GTGGTGGCAACAAGATCAAC+AGG 0.470799 7:+93796797 MsG0780041676.01.T01:CDS
CTTGTCCTCTCCGTCAGGCG+TGG 0.483822 7:+93797019 MsG0780041676.01.T01:CDS
GAGTTAGCTACCATAAGAAA+AGG 0.502671 7:+93797178 MsG0780041676.01.T01:CDS
TAGTCTTCCCAAATTGATCA+AGG 0.503689 7:-93796939 None:intergenic
GCTTATGATCAAGAGATTCA+TGG 0.527780 7:+93797078 MsG0780041676.01.T01:CDS
ATTGAAGCAGAAAGTGGTAC+TGG 0.535622 7:-93796880 None:intergenic
GTAGTTGTGATGTTGCCGTT+AGG 0.535701 7:-93797207 None:intergenic
TCACTTCCATTGTAGCTGCT+AGG 0.535763 7:-93796769 None:intergenic
TTATACAAGAAATATGGCAT+TGG 0.537262 7:+93796726 None:intergenic
CAAGGCTACCCCACGCCTGA+CGG 0.545353 7:-93797029 None:intergenic
CTTTCTGCTTCAATGTGCAA+TGG 0.545802 7:+93796889 MsG0780041676.01.T01:CDS
AGAGGACAAGCACATGGAGA+TGG 0.557220 7:-93797006 None:intergenic
CAAATTGATCAAGGTAGTGA+AGG 0.557585 7:-93796930 None:intergenic
GGGTTTGGCATCCCAAAGAA+AGG 0.560750 7:-93796982 None:intergenic
ACAAACCCTAGCAGCTACAA+TGG 0.562580 7:+93796763 MsG0780041676.01.T01:CDS
TTGCACATTGAAGCAGAAAG+TGG 0.564133 7:-93796886 None:intergenic
GGTAGCCTTGATGCTCTCAT+CGG 0.565716 7:+93797042 MsG0780041676.01.T01:CDS
TAACAGCACCGTCACCAAAA+GGG 0.567030 7:-93797110 None:intergenic
CATGACAAATAGAAATCAAG+AGG 0.576026 7:-93797241 None:intergenic
TGTCCTCTCCGTCAGGCGTG+GGG 0.576278 7:+93797021 MsG0780041676.01.T01:CDS
GGACAAGCACATGGAGATGG+AGG 0.578694 7:-93797003 None:intergenic
GCCTTGATGCTCTCATCGGT+CGG 0.581742 7:+93797046 MsG0780041676.01.T01:CDS
ATCCCAAAGAAAGGACAAGT+TGG 0.587746 7:-93796973 None:intergenic
CAGAAAGTGGTACTGGTCGA+TGG 0.588396 7:-93796873 None:intergenic
CTACCCCACGCCTGACGGAG+AGG 0.603590 7:-93797024 None:intergenic
ACAGGTACGAGAGCCAAAAG+AGG 0.613687 7:+93796815 MsG0780041676.01.T01:CDS
TGACGGAGAGGACAAGCACA+TGG 0.624357 7:-93797012 None:intergenic
AATGGAAGTGATTCAAGTGG+TGG 0.625867 7:+93796781 MsG0780041676.01.T01:CDS
GCCGACCGATGAGAGCATCA+AGG 0.637841 7:-93797047 None:intergenic
AAGAAAAGGAAGAGACCTAA+CGG 0.638455 7:+93797192 MsG0780041676.01.T01:CDS
GACAAGCACATGGAGATGGA+GGG 0.652537 7:-93797002 None:intergenic
TACAATGGAAGTGATTCAAG+TGG 0.673612 7:+93796778 MsG0780041676.01.T01:CDS
TATGATCAAGAGATTCATGG+TGG 0.715975 7:+93797081 MsG0780041676.01.T01:CDS
TCTCATCGGTCGGCTCCGCG+CGG 0.722452 7:+93797056 MsG0780041676.01.T01:CDS
TCTCTTGATCATAAGCCGCG+CGG 0.776146 7:-93797071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CATGACAAATAGAAATCAAG+AGG - Chr7:93797244-93797263 None:intergenic 30.0%
TTCACTACCTTGATCAATTT+GGG + Chr7:93796932-93796951 MsG0780041676.01.T01:CDS 30.0%
AAGAAAAGGAAGAGACCTAA+CGG + Chr7:93797192-93797211 MsG0780041676.01.T01:CDS 35.0%
AATCCAACTTGTCCTTTCTT+TGG + Chr7:93796970-93796989 MsG0780041676.01.T01:CDS 35.0%
ATCCAACTTGTCCTTTCTTT+GGG + Chr7:93796971-93796990 MsG0780041676.01.T01:CDS 35.0%
CAAATTGATCAAGGTAGTGA+AGG - Chr7:93796933-93796952 None:intergenic 35.0%
CTTCACTACCTTGATCAATT+TGG + Chr7:93796931-93796950 MsG0780041676.01.T01:CDS 35.0%
GAGTTAGCTACCATAAGAAA+AGG + Chr7:93797178-93797197 MsG0780041676.01.T01:CDS 35.0%
GCTTATGATCAAGAGATTCA+TGG + Chr7:93797078-93797097 MsG0780041676.01.T01:CDS 35.0%
GTCGATGGTTTCTTAAATAC+TGG - Chr7:93796861-93796880 None:intergenic 35.0%
TACAATGGAAGTGATTCAAG+TGG + Chr7:93796778-93796797 MsG0780041676.01.T01:CDS 35.0%
TAGTCTTCCCAAATTGATCA+AGG - Chr7:93796942-93796961 None:intergenic 35.0%
TATGATCAAGAGATTCATGG+TGG + Chr7:93797081-93797100 MsG0780041676.01.T01:CDS 35.0%
TGATCAATTTGGGAAGACTA+AGG + Chr7:93796942-93796961 MsG0780041676.01.T01:CDS 35.0%
! TAGGTCTCTTCCTTTTCTTA+TGG - Chr7:93797191-93797210 None:intergenic 35.0%
! TGTTCGTGATTTTCAGTCAA+AGG + Chr7:93797149-93797168 MsG0780041676.01.T01:CDS 35.0%
AATGGAAGTGATTCAAGTGG+TGG + Chr7:93796781-93796800 MsG0780041676.01.T01:CDS 40.0%
ATCCCAAAGAAAGGACAAGT+TGG - Chr7:93796976-93796995 None:intergenic 40.0%
ATTGAAGCAGAAAGTGGTAC+TGG - Chr7:93796883-93796902 None:intergenic 40.0%
CTTTCTGCTTCAATGTGCAA+TGG + Chr7:93796889-93796908 MsG0780041676.01.T01:CDS 40.0%
TTGCACATTGAAGCAGAAAG+TGG - Chr7:93796889-93796908 None:intergenic 40.0%
! GATTTTCAGTCAAAGGCTAG+AGG + Chr7:93797156-93797175 MsG0780041676.01.T01:CDS 40.0%
! TGTTCCAATCTCTCCTCTTT+TGG - Chr7:93796831-93796850 None:intergenic 40.0%
ACAAACCCTAGCAGCTACAA+TGG + Chr7:93796763-93796782 MsG0780041676.01.T01:CDS 45.0%
AGAGCCAAAAGAGGAGAGAT+TGG + Chr7:93796824-93796843 MsG0780041676.01.T01:CDS 45.0%
CACTTCCATTGTAGCTGCTA+GGG - Chr7:93796771-93796790 None:intergenic 45.0%
GTAGTTGTGATGTTGCCGTT+AGG - Chr7:93797210-93797229 None:intergenic 45.0%
TAACAGCACCGTCACCAAAA+GGG - Chr7:93797113-93797132 None:intergenic 45.0%
TCACTTCCATTGTAGCTGCT+AGG - Chr7:93796772-93796791 None:intergenic 45.0%
! GGAAGTAACCCTTTTGGTGA+CGG + Chr7:93797102-93797121 MsG0780041676.01.T01:CDS 45.0%
!! CATGGTGGAAGTAACCCTTT+TGG + Chr7:93797096-93797115 MsG0780041676.01.T01:CDS 45.0%
ACAGGTACGAGAGCCAAAAG+AGG + Chr7:93796815-93796834 MsG0780041676.01.T01:CDS 50.0%
AGAGGACAAGCACATGGAGA+TGG - Chr7:93797009-93797028 None:intergenic 50.0%
CTAACAGCACCGTCACCAAA+AGG - Chr7:93797114-93797133 None:intergenic 50.0%
GACAAGCACATGGAGATGGA+GGG - Chr7:93797005-93797024 None:intergenic 50.0%
GGGTTTGGCATCCCAAAGAA+AGG - Chr7:93796985-93797004 None:intergenic 50.0%
GTGGTGGCAACAAGATCAAC+AGG + Chr7:93796797-93796816 MsG0780041676.01.T01:CDS 50.0%
TCTCTTGATCATAAGCCGCG+CGG - Chr7:93797074-93797093 None:intergenic 50.0%
! GGTAGCCTTGATGCTCTCAT+CGG + Chr7:93797042-93797061 MsG0780041676.01.T01:CDS 50.0%
!! CAGAAAGTGGTACTGGTCGA+TGG - Chr7:93796876-93796895 None:intergenic 50.0%
!!! CTTTTGGTGACGGTGCTGTT+AGG + Chr7:93797112-93797131 MsG0780041676.01.T01:CDS 50.0%
ATGTGCTTGTCCTCTCCGTC+AGG + Chr7:93797014-93797033 MsG0780041676.01.T01:CDS 55.0%
GCACATGGAGATGGAGGGTT+TGG - Chr7:93797000-93797019 None:intergenic 55.0%
GCCTTGATGCTCTCATCGGT+CGG + Chr7:93797046-93797065 MsG0780041676.01.T01:CDS 55.0%
GGACAAGCACATGGAGATGG+AGG - Chr7:93797006-93797025 None:intergenic 55.0%
TGACGGAGAGGACAAGCACA+TGG - Chr7:93797015-93797034 None:intergenic 55.0%
TTGTCCTCTCCGTCAGGCGT+GGG + Chr7:93797020-93797039 MsG0780041676.01.T01:CDS 60.0%
!! GCCGACCGATGAGAGCATCA+AGG - Chr7:93797050-93797069 None:intergenic 60.0%
CAAGGCTACCCCACGCCTGA+CGG - Chr7:93797032-93797051 None:intergenic 65.0%
CTTGTCCTCTCCGTCAGGCG+TGG + Chr7:93797019-93797038 MsG0780041676.01.T01:CDS 65.0%
TGTCCTCTCCGTCAGGCGTG+GGG + Chr7:93797021-93797040 MsG0780041676.01.T01:CDS 65.0%
CTACCCCACGCCTGACGGAG+AGG - Chr7:93797027-93797046 None:intergenic 70.0%
! TCTCATCGGTCGGCTCCGCG+CGG + Chr7:93797056-93797075 MsG0780041676.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 93796739 93797264 93796739 ID=MsG0780041676.01;Name=MsG0780041676.01
Chr7 mRNA 93796739 93797248 93796739 ID=MsG0780041676.01.T01;Parent=MsG0780041676.01;Name=MsG0780041676.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|169
Chr7 exon 93796739 93797248 93796739 ID=MsG0780041676.01.T01:exon:22272;Parent=MsG0780041676.01.T01
Chr7 CDS 93796739 93797248 93796739 ID=MsG0780041676.01.T01:cds;Parent=MsG0780041676.01.T01
Chr7 mRNA 93796901 93797248 93796901 ID=MsG0780041676.01.T02;Parent=MsG0780041676.01;Name=MsG0780041676.01.T02;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|0|1|0|115
Chr7 exon 93796901 93797248 93796901 ID=MsG0780041676.01.T02:exon:22273;Parent=MsG0780041676.01.T02
Chr7 CDS 93796901 93797248 93796901 ID=MsG0780041676.01.T02:cds;Parent=MsG0780041676.01.T02
Chr7 mRNA 93797082 93797264 93797082 ID=MsG0780041676.01.T03;Parent=MsG0780041676.01;Name=MsG0780041676.01.T03;_AED=0.41;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|60
Chr7 exon 93797082 93797264 93797082 ID=MsG0780041676.01.T03:exon:22274;Parent=MsG0780041676.01.T03
Chr7 CDS 93797082 93797264 93797082 ID=MsG0780041676.01.T03:cds;Parent=MsG0780041676.01.T03
Gene Sequence

>MsG0780041676.01.T02

ATGTGCAATGGAACACATGTTCTTGAATTCCTTCACTACCTTGATCAATTTGGGAAGACTAAGGTTCACAATCCAACTTGTCCTTTCTTTGGGATGCCAAACCCTCCATCTCCATGTGCTTGTCCTCTCCGTCAGGCGTGGGGTAGCCTTGATGCTCTCATCGGTCGGCTCCGCGCGGCTTATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGACGGTGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACGGCAACATCACAACTACTTCTCAATCATCCTCTTGA

>MsG0780041676.01.T01

ATGGCATTGGTTTCAGCTGAATCAACAAACCCTAGCAGCTACAATGGAAGTGATTCAAGTGGTGGCAACAAGATCAACAGGTACGAGAGCCAAAAGAGGAGAGATTGGAACACATTCTGCCAGTATTTAAGAAACCATCGACCAGTACCACTTTCTGCTTCAATGTGCAATGGAACACATGTTCTTGAATTCCTTCACTACCTTGATCAATTTGGGAAGACTAAGGTTCACAATCCAACTTGTCCTTTCTTTGGGATGCCAAACCCTCCATCTCCATGTGCTTGTCCTCTCCGTCAGGCGTGGGGTAGCCTTGATGCTCTCATCGGTCGGCTCCGCGCGGCTTATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGACGGTGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACGGCAACATCACAACTACTTCTCAATCATCCTCTTGA

>MsG0780041676.01.T03

ATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGACGGTGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACGGCAACATCACAACTACTTCTCAATCATCCTCTTGATTTCTATTTGTCATGA

Protein sequence

>MsG0780041676.01.T02

MCNGTHVLEFLHYLDQFGKTKVHNPTCPFFGMPNPPSPCACPLRQAWGSLDALIGRLRAAYDQEIHGGSNPFGDGAVRFYLRDVRDFQSKARGVSYHKKRKRPNGNITTTSQSSS*

>MsG0780041676.01.T01

MALVSAESTNPSSYNGSDSSGGNKINRYESQKRRDWNTFCQYLRNHRPVPLSASMCNGTHVLEFLHYLDQFGKTKVHNPTCPFFGMPNPPSPCACPLRQAWGSLDALIGRLRAAYDQEIHGGSNPFGDGAVRFYLRDVRDFQSKARGVSYHKKRKRPNGNITTTSQSSS*

>MsG0780041676.01.T03

MIKRFMVEVTLLVTVLLGFTCVMFVIFSQRLEELATIRKGRDLTATSQLLLNHPLDFYLS*