Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041791.01.T01 | AES82811.1 | 94.231 | 104 | 5 | 1 | 1 | 103 | 1 | 104 | 1.11E-63 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041791.01.T01 | G7L533 | 94.231 | 104 | 5 | 1 | 1 | 103 | 1 | 104 | 5.32e-64 | 198 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049040.01 | MsG0780041791.01 | 0.830408 | 2.987029e-55 | 3.144307e-52 |
| MsG0180001100.01 | MsG0780041791.01 | 0.806153 | 9.445163e-50 | 5.089504e-47 |
| MsG0180001270.01 | MsG0780041791.01 | 0.800468 | 1.421161e-48 | 6.622102e-46 |
| MsG0180005039.01 | MsG0780041791.01 | 0.801448 | 8.963205e-49 | 4.281544e-46 |
| MsG0180005807.01 | MsG0780041791.01 | 0.805239 | 1.469172e-49 | 7.733052e-47 |
| MsG0780040430.01 | MsG0780041791.01 | 0.802494 | 5.462565e-49 | 2.679591e-46 |
| MsG0780040431.01 | MsG0780041791.01 | 0.805928 | 1.053384e-49 | 5.643666e-47 |
| MsG0780041791.01 | MsG0880045558.01 | 0.830525 | 2.796475e-55 | 2.953670e-52 |
| MsG0780041791.01 | MsG0880047168.01 | 0.801903 | 7.228567e-49 | 3.493039e-46 |
| MsG0280010569.01 | MsG0780041791.01 | 0.803875 | 2.828536e-49 | 1.437556e-46 |
| MsG0680030784.01 | MsG0780041791.01 | 0.820723 | 5.898731e-53 | 4.704289e-50 |
| MsG0680030908.01 | MsG0780041791.01 | 0.814068 | 1.859516e-51 | 1.235295e-48 |
| MsG0680031469.01 | MsG0780041791.01 | 0.838009 | 3.707952e-57 | 4.902453e-54 |
| MsG0680032830.01 | MsG0780041791.01 | 0.804535 | 2.062121e-49 | 1.065847e-46 |
| MsG0680034064.01 | MsG0780041791.01 | 0.807582 | 4.712081e-50 | 2.634204e-47 |
| MsG0680034767.01 | MsG0780041791.01 | 0.803659 | 3.136793e-49 | 1.585239e-46 |
| MsG0480018848.01 | MsG0780041791.01 | 0.828794 | 7.374425e-55 | 7.405706e-52 |
| MsG0480019340.01 | MsG0780041791.01 | 0.805147 | 1.535946e-49 | 8.065608e-47 |
| MsG0480020315.01 | MsG0780041791.01 | 0.842017 | 3.342354e-58 | 5.000881e-55 |
| MsG0480020783.01 | MsG0780041791.01 | 0.840538 | 8.187429e-58 | 1.169845e-54 |
| MsG0480021814.01 | MsG0780041791.01 | 0.826136 | 3.201624e-54 | 2.976815e-51 |
| MsG0480022537.01 | MsG0780041791.01 | 0.800410 | 1.460404e-48 | 6.795333e-46 |
| MsG0480022647.01 | MsG0780041791.01 | 0.810309 | 1.229233e-50 | 7.384196e-48 |
| MsG0480023367.01 | MsG0780041791.01 | 0.805330 | 1.406340e-49 | 7.419642e-47 |
| MsG0480023990.01 | MsG0780041791.01 | 0.812555 | 3.996212e-51 | 2.548776e-48 |
| MsG0480023992.01 | MsG0780041791.01 | 0.822191 | 2.702705e-53 | 2.246153e-50 |
| MsG0580025502.01 | MsG0780041791.01 | 0.818072 | 2.372440e-52 | 1.757957e-49 |
| MsG0580025668.01 | MsG0780041791.01 | 0.824133 | 9.518297e-54 | 8.357983e-51 |
| MsG0580025828.01 | MsG0780041791.01 | 0.805524 | 1.280856e-49 | 6.791127e-47 |
| MsG0380016192.01 | MsG0780041791.01 | 0.816520 | 5.302299e-52 | 3.765440e-49 |
| MsG0380017087.01 | MsG0780041791.01 | 0.819705 | 1.009249e-52 | 7.824705e-50 |
| MsG0380017088.01 | MsG0780041791.01 | 0.800048 | 1.729806e-48 | 7.975110e-46 |
| MsG0580027655.01 | MsG0780041791.01 | 0.804218 | 2.400193e-49 | 1.230540e-46 |
| MsG0580027725.01 | MsG0780041791.01 | 0.818461 | 1.936628e-52 | 1.450692e-49 |
| MsG0580027726.01 | MsG0780041791.01 | 0.809629 | 1.721773e-50 | 1.016136e-47 |
| MsG0580027763.01 | MsG0780041791.01 | 0.817770 | 2.776034e-52 | 2.040198e-49 |
| MsG0580028335.01 | MsG0780041791.01 | 0.805144 | 1.538057e-49 | 8.076089e-47 |
| MsG0280007075.01 | MsG0780041791.01 | 0.830003 | 3.751360e-55 | 3.902879e-52 |
| MsG0280010503.01 | MsG0780041791.01 | 0.818526 | 1.871779e-52 | 1.404562e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041791.01.T01 | MTR_7g118070 | 94.231 | 104 | 5 | 1 | 1 | 103 | 1 | 104 | 1.35e-67 | 198 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAAGGGGCTGCGTTTA+AGG | 0.199352 | 7:-95182524 | MsG0780041791.01.T01:CDS |
| GGCCAACTCAAGGATTTAAT+AGG | 0.281462 | 7:-95182672 | MsG0780041791.01.T01:CDS |
| AACAATGACAATTTAGAATT+AGG | 0.285252 | 7:-95182636 | MsG0780041791.01.T01:CDS |
| CTGTGGTAGTTCACCGCATT+CGG | 0.308570 | 7:-95182731 | MsG0780041791.01.T01:CDS |
| TCTTCGCTATGTTCTTGAAT+TGG | 0.381899 | 7:+95182501 | None:intergenic |
| TAAATCCTTGAGTTGGCCTT+TGG | 0.387136 | 7:+95182677 | None:intergenic |
| AAAATGGGTAATGCTTGCTT+TGG | 0.394791 | 7:-95182786 | MsG0780041791.01.T01:CDS |
| GCCAACTCAAGGATTTAATA+GGG | 0.437318 | 7:-95182671 | MsG0780041791.01.T01:CDS |
| TGTGGTAGTTCACCGCATTC+GGG | 0.441159 | 7:-95182730 | MsG0780041791.01.T01:CDS |
| ACGAGCACGAAGCCTCCCTT+TGG | 0.474923 | 7:+95182590 | None:intergenic |
| TCCCTATTAAATCCTTGAGT+TGG | 0.476466 | 7:+95182670 | None:intergenic |
| AGCATTCTTGAACGATCAAA+TGG | 0.501702 | 7:+95182613 | None:intergenic |
| GGATCAAGCCGTGACGATCA+AGG | 0.532320 | 7:-95182709 | MsG0780041791.01.T01:CDS |
| CGAATGCGGTGAACTACCAC+AGG | 0.532604 | 7:+95182732 | None:intergenic |
| ATCGTTCAAGAATGCTCCAA+AGG | 0.558651 | 7:-95182606 | MsG0780041791.01.T01:CDS |
| CGTGTTGTAACTGCTGCAGT+TGG | 0.558826 | 7:-95182570 | MsG0780041791.01.T01:CDS |
| CAAGGATTTAATAGGGAAGG+TGG | 0.561353 | 7:-95182664 | MsG0780041791.01.T01:CDS |
| TCGTTCAAGAATGCTCCAAA+GGG | 0.578470 | 7:-95182605 | MsG0780041791.01.T01:CDS |
| CATTCTCACCTTGATCGTCA+CGG | 0.582038 | 7:+95182701 | None:intergenic |
| GAAGATGAGCAACAAGGGAG+TGG | 0.588540 | 7:-95182760 | MsG0780041791.01.T01:CDS |
| GGAAAGAAGATGAGCAACAA+GGG | 0.598826 | 7:-95182765 | MsG0780041791.01.T01:CDS |
| TGGAAAGAAGATGAGCAACA+AGG | 0.599291 | 7:-95182766 | MsG0780041791.01.T01:CDS |
| AGAACATAGCGAAGATGTGT+AGG | 0.601986 | 7:-95182493 | None:intergenic |
| AATGACCAAAGGCCAACTCA+AGG | 0.609242 | 7:-95182682 | MsG0780041791.01.T01:CDS |
| TTCAAGAATGCTCCAAAGGG+AGG | 0.614086 | 7:-95182602 | MsG0780041791.01.T01:CDS |
| ACTCAAGGATTTAATAGGGA+AGG | 0.618810 | 7:-95182667 | MsG0780041791.01.T01:CDS |
| TCACGGCTTGATCCCGAATG+CGG | 0.664295 | 7:+95182718 | None:intergenic |
| CAAGGGAGTGGTGCACCCTG+TGG | 0.671108 | 7:-95182748 | MsG0780041791.01.T01:CDS |
| ATCAAGGTGAGAATGACCAA+AGG | 0.688055 | 7:-95182693 | MsG0780041791.01.T01:CDS |
| GAATGCGGTGAACTACCACA+GGG | 0.775194 | 7:+95182733 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATGACAATTTAGAATT+AGG | - | Chr7:95182641-95182660 | MsG0780041791.01.T01:CDS | 20.0% |
| ! | ATACGTACAAGTTTTCAAAG+GGG | - | Chr7:95182741-95182760 | MsG0780041791.01.T01:CDS | 30.0% |
| ! | CATACGTACAAGTTTTCAAA+GGG | - | Chr7:95182740-95182759 | MsG0780041791.01.T01:CDS | 30.0% |
| ! | TCATACGTACAAGTTTTCAA+AGG | - | Chr7:95182739-95182758 | MsG0780041791.01.T01:CDS | 30.0% |
| ACTCAAGGATTTAATAGGGA+AGG | - | Chr7:95182610-95182629 | MsG0780041791.01.T01:CDS | 35.0% | |
| AGCATTCTTGAACGATCAAA+TGG | + | Chr7:95182667-95182686 | None:intergenic | 35.0% | |
| GCCAACTCAAGGATTTAATA+GGG | - | Chr7:95182606-95182625 | MsG0780041791.01.T01:CDS | 35.0% | |
| TCCCTATTAAATCCTTGAGT+TGG | + | Chr7:95182610-95182629 | None:intergenic | 35.0% | |
| TCTTCGCTATGTTCTTGAAT+TGG | + | Chr7:95182779-95182798 | None:intergenic | 35.0% | |
| ATCAAGGTGAGAATGACCAA+AGG | - | Chr7:95182584-95182603 | MsG0780041791.01.T01:CDS | 40.0% | |
| ATCGTTCAAGAATGCTCCAA+AGG | - | Chr7:95182671-95182690 | MsG0780041791.01.T01:CDS | 40.0% | |
| CAAGGATTTAATAGGGAAGG+TGG | - | Chr7:95182613-95182632 | MsG0780041791.01.T01:CDS | 40.0% | |
| GGAAAGAAGATGAGCAACAA+GGG | - | Chr7:95182512-95182531 | MsG0780041791.01.T01:CDS | 40.0% | |
| GGCCAACTCAAGGATTTAAT+AGG | - | Chr7:95182605-95182624 | MsG0780041791.01.T01:CDS | 40.0% | |
| TCGTTCAAGAATGCTCCAAA+GGG | - | Chr7:95182672-95182691 | MsG0780041791.01.T01:CDS | 40.0% | |
| TGGAAAGAAGATGAGCAACA+AGG | - | Chr7:95182511-95182530 | MsG0780041791.01.T01:CDS | 40.0% | |
| !! | TAAATCCTTGAGTTGGCCTT+TGG | + | Chr7:95182603-95182622 | None:intergenic | 40.0% |
| AATGACCAAAGGCCAACTCA+AGG | - | Chr7:95182595-95182614 | MsG0780041791.01.T01:CDS | 45.0% | |
| CATTCTCACCTTGATCGTCA+CGG | + | Chr7:95182579-95182598 | None:intergenic | 45.0% | |
| TTCAAGAATGCTCCAAAGGG+AGG | - | Chr7:95182675-95182694 | MsG0780041791.01.T01:CDS | 45.0% | |
| TTTCAAAGGGGCTGCGTTTA+AGG | - | Chr7:95182753-95182772 | MsG0780041791.01.T01:CDS | 45.0% | |
| CGTGTTGTAACTGCTGCAGT+TGG | - | Chr7:95182707-95182726 | MsG0780041791.01.T01:CDS | 50.0% | |
| CTGTGGTAGTTCACCGCATT+CGG | - | Chr7:95182546-95182565 | MsG0780041791.01.T01:CDS | 50.0% | |
| GAAGATGAGCAACAAGGGAG+TGG | - | Chr7:95182517-95182536 | MsG0780041791.01.T01:CDS | 50.0% | |
| GAATGCGGTGAACTACCACA+GGG | + | Chr7:95182547-95182566 | None:intergenic | 50.0% | |
| TGTGGTAGTTCACCGCATTC+GGG | - | Chr7:95182547-95182566 | MsG0780041791.01.T01:CDS | 50.0% | |
| CGAATGCGGTGAACTACCAC+AGG | + | Chr7:95182548-95182567 | None:intergenic | 55.0% | |
| GGATCAAGCCGTGACGATCA+AGG | - | Chr7:95182568-95182587 | MsG0780041791.01.T01:CDS | 55.0% | |
| TCACGGCTTGATCCCGAATG+CGG | + | Chr7:95182562-95182581 | None:intergenic | 55.0% | |
| ACGAGCACGAAGCCTCCCTT+TGG | + | Chr7:95182690-95182709 | None:intergenic | 60.0% | |
| CAAGGGAGTGGTGCACCCTG+TGG | - | Chr7:95182529-95182548 | MsG0780041791.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 95182494 | 95182805 | 95182494 | ID=MsG0780041791.01;Name=MsG0780041791.01 |
| Chr7 | mRNA | 95182494 | 95182805 | 95182494 | ID=MsG0780041791.01.T01;Parent=MsG0780041791.01;Name=MsG0780041791.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr7 | exon | 95182494 | 95182805 | 95182494 | ID=MsG0780041791.01.T01:exon:22764;Parent=MsG0780041791.01.T01 |
| Chr7 | CDS | 95182494 | 95182805 | 95182494 | ID=MsG0780041791.01.T01:cds;Parent=MsG0780041791.01.T01 |
| Gene Sequence |
| Protein sequence |
