AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017087.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAACAATTATAATTAC+TGG 0.255515 3:-91785186 MsG0380017087.01.T01:CDS
AAAAGAGAAAGAACAAAGTT+AGG 0.311037 3:+91785220 None:intergenic
TATCAAGAGACATGTGCTTA+AGG 0.397983 3:-91785282 MsG0380017087.01.T01:CDS
AAAGAGAAAGAACAAAGTTA+GGG 0.403160 3:+91785221 None:intergenic
AGAGACATGTGCTTAAGGTT+TGG 0.408486 3:-91785277 MsG0380017087.01.T01:CDS
TACTGGATTTCAGTGTCAAT+AGG 0.426645 3:-91785169 MsG0380017087.01.T01:CDS
CACAATAACAAGCACACTCT+TGG 0.453753 3:-91785251 MsG0380017087.01.T01:CDS
AAATGGGTGGAGAATTCAGA+AGG 0.589294 3:-91785345 MsG0380017087.01.T01:CDS
CAGTGTCAATAGGACTAGTG+TGG 0.590165 3:-91785159 MsG0380017087.01.T01:CDS
AGAATTCAGAAGGAAGATGA+AGG 0.605493 3:-91785335 MsG0380017087.01.T01:CDS
TGCATTTGCGGAGAAATGGG+TGG 0.624944 3:-91785358 None:intergenic
AGTGTCAATAGGACTAGTGT+GGG 0.645961 3:-91785158 MsG0380017087.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAGAACAATTATAATTAC+TGG - Chr3:91785298-91785317 MsG0380017087.01.T01:CDS 20.0%
! AAAAGAGAAAGAACAAAGTT+AGG + Chr3:91785267-91785286 None:intergenic 25.0%
! AAAGAGAAAGAACAAAGTTA+GGG + Chr3:91785266-91785285 None:intergenic 25.0%
AGAATTCAGAAGGAAGATGA+AGG - Chr3:91785149-91785168 MsG0380017087.01.T01:CDS 35.0%
TACTGGATTTCAGTGTCAAT+AGG - Chr3:91785315-91785334 MsG0380017087.01.T01:CDS 35.0%
TATCAAGAGACATGTGCTTA+AGG - Chr3:91785202-91785221 MsG0380017087.01.T01:CDS 35.0%
AGAGACATGTGCTTAAGGTT+TGG - Chr3:91785207-91785226 MsG0380017087.01.T01:CDS 40.0%
CACAATAACAAGCACACTCT+TGG - Chr3:91785233-91785252 MsG0380017087.01.T01:CDS 40.0%
! AGTGTCAATAGGACTAGTGT+GGG - Chr3:91785326-91785345 MsG0380017087.01.T01:CDS 40.0%
! CAGTGTCAATAGGACTAGTG+TGG - Chr3:91785325-91785344 MsG0380017087.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 91785141 91785365 91785141 ID=MsG0380017087.01;Name=MsG0380017087.01
Chr3 mRNA 91785141 91785365 91785141 ID=MsG0380017087.01.T01;Parent=MsG0380017087.01;Name=MsG0380017087.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|74
Chr3 exon 91785141 91785365 91785141 ID=MsG0380017087.01.T01:exon:31027;Parent=MsG0380017087.01.T01
Chr3 CDS 91785141 91785365 91785141 ID=MsG0380017087.01.T01:cds;Parent=MsG0380017087.01.T01
Gene Sequence

>MsG0380017087.01.T01

ATGGGTGGAGAATTCAGAAGGAAGATGAAGGTGCAATCGAACAATTCTGTGCTGACAATTTTATCAAGAGACATGTGCTTAAGGTTTGGATGCACAATAACAAGCACACTCTTGGTAAGAAACCCTAACTTTGTTCTTTCTCTTTTTTTCAGTAATTTTGAGAACAATTATAATTACTGGATTTCAGTGTCAATAGGACTAGTGTGGGACATCATGAGTTTATGA

Protein sequence

>MsG0380017087.01.T01

MGGEFRRKMKVQSNNSVLTILSRDMCLRFGCTITSTLLVRNPNFVLSLFFSNFENNYNYWISVSIGLVWDIMSL*