AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017088.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACATGCCACCGGCGTAAC+AGG 0.280829 3:+91785953 None:intergenic
TTGTATCTGACCGTTCCTGC+TGG 0.450197 3:+91785833 None:intergenic
AAATGGGTTCCCTGTTACGC+CGG 0.483195 3:-91785963 MsG0380017088.01.T01:CDS
CGCTTGGAAACTCAGGAGTT+AGG 0.485005 3:-91785925 MsG0380017088.01.T01:CDS
TTCCAGTGTTCCTTCTTCTC+CGG 0.499051 3:+91785739 None:intergenic
ACAGGCGGTGGTGTCAAAAG+TGG 0.503495 3:-91785857 MsG0380017088.01.T01:CDS
GGTGGAATCGGTTCAGCGGA+CGG 0.518412 3:-91785890 MsG0380017088.01.T01:CDS
GGTGTCAAAAGTGGGCCAGC+AGG 0.527350 3:-91785848 MsG0380017088.01.T01:CDS
AGAAGAAGGAACACTGGAAG+CGG 0.543572 3:-91785735 MsG0380017088.01.T02:CDS
CAAAAGTGGGCCAGCAGGAA+CGG 0.546267 3:-91785843 MsG0380017088.01.T01:CDS
GGAGGAGCAGCCACAGGCGG+TGG 0.554790 3:-91785869 MsG0380017088.01.T01:CDS
GTGGCATGTTACGACTCGCT+TGG 0.557648 3:-91785941 MsG0380017088.01.T01:CDS
AAGAATCACGCCGTCAGCAT+AGG 0.558884 3:-91785800 MsG0380017088.01.T02:CDS
GCGGACGGAGGAGCAGCCAC+AGG 0.565208 3:-91785875 MsG0380017088.01.T01:CDS
TGGGTTCCCTGTTACGCCGG+TGG 0.568935 3:-91785960 MsG0380017088.01.T01:CDS
TACGACTCGCTTGGAAACTC+AGG 0.569322 3:-91785932 MsG0380017088.01.T01:CDS
AATCACGCCGTCAGCATAGG+TGG 0.574956 3:-91785797 MsG0380017088.01.T02:CDS
ATAGGTGGTCACGCCGTGGA+TGG 0.576462 3:-91785782 MsG0380017088.01.T02:CDS
CAGGCGGTGGTGTCAAAAGT+GGG 0.580393 3:-91785856 MsG0380017088.01.T01:CDS
AACATGCCACCGGCGTAACA+GGG 0.586523 3:+91785954 None:intergenic
AGCGAGTCGTAACATGCCAC+CGG 0.591718 3:+91785944 None:intergenic
GACGGAGGAGCAGCCACAGG+CGG 0.606719 3:-91785872 MsG0380017088.01.T01:CDS
GGCGTGACCACCTATGCTGA+CGG 0.619665 3:+91785790 None:intergenic
CAGCATAGGTGGTCACGCCG+TGG 0.632841 3:-91785786 MsG0380017088.01.T02:CDS
TGGCGGTGGAATCGGTTCAG+CGG 0.633285 3:-91785894 MsG0380017088.01.T01:CDS
TGCCGGAGAAGAAGGAACAC+TGG 0.644421 3:-91785741 MsG0380017088.01.T02:CDS
GGAATCGGTTCAGCGGACGG+AGG 0.678169 3:-91785887 MsG0380017088.01.T01:CDS
AAACTCGCAACATCCATCCA+CGG 0.687116 3:+91785769 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCAGGAGTTAGGTCCAAAAA+TGG - Chr3:91785763-91785782 MsG0380017088.01.T02:CDS 40.0%
AAACTCGCAACATCCATCCA+CGG + Chr3:91785911-91785930 None:intergenic 45.0%
AGAAGAAGGAACACTGGAAG+CGG - Chr3:91785942-91785961 MsG0380017088.01.T01:CDS 45.0%
GGAGTTAGGTCCAAAAATGG+CGG - Chr3:91785766-91785785 MsG0380017088.01.T02:CDS 45.0%
TCCAAAAATGGCGGTGGAAT+CGG - Chr3:91785775-91785794 MsG0380017088.01.T02:CDS 45.0%
TTCCAGTGTTCCTTCTTCTC+CGG + Chr3:91785941-91785960 None:intergenic 45.0%
!!! TGTTGCGAGTTTTTAGCTGC+CGG - Chr3:91785919-91785938 MsG0380017088.01.T01:CDS 45.0%
!!! TTTTTAGCTGCCGGAGAAGA+AGG - Chr3:91785928-91785947 MsG0380017088.01.T01:CDS 45.0%
AAGAATCACGCCGTCAGCAT+AGG - Chr3:91785877-91785896 MsG0380017088.01.T01:CDS 50.0%
CGCTTGGAAACTCAGGAGTT+AGG - Chr3:91785752-91785771 MsG0380017088.01.T02:CDS 50.0%
GTTAGGTCCAAAAATGGCGG+TGG - Chr3:91785769-91785788 MsG0380017088.01.T02:CDS 50.0%
TACGACTCGCTTGGAAACTC+AGG - Chr3:91785745-91785764 MsG0380017088.01.T02:CDS 50.0%
TTGTATCTGACCGTTCCTGC+TGG + Chr3:91785847-91785866 None:intergenic 50.0%
!! ACCGATTCCACCGCCATTTT+TGG + Chr3:91785779-91785798 None:intergenic 50.0%
AACATGCCACCGGCGTAACA+GGG + Chr3:91785726-91785745 None:intergenic 55.0%
AATCACGCCGTCAGCATAGG+TGG - Chr3:91785880-91785899 MsG0380017088.01.T01:CDS 55.0%
AGCGAGTCGTAACATGCCAC+CGG + Chr3:91785736-91785755 None:intergenic 55.0%
CAAAAGTGGGCCAGCAGGAA+CGG - Chr3:91785834-91785853 MsG0380017088.01.T01:CDS 55.0%
GTGGCATGTTACGACTCGCT+TGG - Chr3:91785736-91785755 MsG0380017088.01.T02:CDS 55.0%
TAACATGCCACCGGCGTAAC+AGG + Chr3:91785727-91785746 None:intergenic 55.0%
TGCCGGAGAAGAAGGAACAC+TGG - Chr3:91785936-91785955 MsG0380017088.01.T01:CDS 55.0%
! ACAGGCGGTGGTGTCAAAAG+TGG - Chr3:91785820-91785839 MsG0380017088.01.T01:CDS 55.0%
! ACTTTTGACACCACCGCCTG+TGG + Chr3:91785821-91785840 None:intergenic 55.0%
! CAGGCGGTGGTGTCAAAAGT+GGG - Chr3:91785821-91785840 MsG0380017088.01.T01:CDS 55.0%
ATAGGTGGTCACGCCGTGGA+TGG - Chr3:91785895-91785914 MsG0380017088.01.T01:CDS 60.0%
GGCGTGACCACCTATGCTGA+CGG + Chr3:91785890-91785909 None:intergenic 60.0%
GGTGTCAAAAGTGGGCCAGC+AGG - Chr3:91785829-91785848 MsG0380017088.01.T01:CDS 60.0%
! GGTGGAATCGGTTCAGCGGA+CGG - Chr3:91785787-91785806 MsG0380017088.01.T02:CDS 60.0%
! TGGCGGTGGAATCGGTTCAG+CGG - Chr3:91785783-91785802 MsG0380017088.01.T02:CDS 60.0%
CAGCATAGGTGGTCACGCCG+TGG - Chr3:91785891-91785910 MsG0380017088.01.T01:CDS 65.0%
TGGGTTCCCTGTTACGCCGG+TGG - Chr3:91785717-91785736 MsG0380017088.01.T02:CDS 65.0%
! GGAATCGGTTCAGCGGACGG+AGG - Chr3:91785790-91785809 MsG0380017088.01.T02:CDS 65.0%
GACGGAGGAGCAGCCACAGG+CGG - Chr3:91785805-91785824 MsG0380017088.01.T01:CDS 70.0%
GCGGACGGAGGAGCAGCCAC+AGG - Chr3:91785802-91785821 MsG0380017088.01.T01:CDS 75.0%
GGAGGAGCAGCCACAGGCGG+TGG - Chr3:91785808-91785827 MsG0380017088.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 91785716 91785983 91785716 ID=MsG0380017088.01;Name=MsG0380017088.01
Chr3 mRNA 91785801 91785983 91785801 ID=MsG0380017088.01.T01;Parent=MsG0380017088.01;Name=MsG0380017088.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|60
Chr3 exon 91785801 91785983 91785801 ID=MsG0380017088.01.T01:exon:31028;Parent=MsG0380017088.01.T01
Chr3 CDS 91785801 91785983 91785801 ID=MsG0380017088.01.T01:cds;Parent=MsG0380017088.01.T01
Chr3 mRNA 91785716 91785829 91785716 ID=MsG0380017088.01.T02;Parent=MsG0380017088.01;Name=MsG0380017088.01.T02;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|37
Chr3 exon 91785716 91785829 91785716 ID=MsG0380017088.01.T02:exon:31029;Parent=MsG0380017088.01.T02
Chr3 CDS 91785716 91785829 91785716 ID=MsG0380017088.01.T02:cds;Parent=MsG0380017088.01.T02
Gene Sequence

>MsG0380017088.01.T02

ATGTCAAAAGAATCACGCCGTCAGCATAGGTGGTCACGCCGTGGATGGATGTTGCGAGTTTTTAGCTGCCGGAGAAGAAGGAACACTGGAAGCGGTGATTTGTGCTGCTTGTAA

>MsG0380017088.01.T01

ATGGGTTCCCTGTTACGCCGGTGGCATGTTACGACTCGCTTGGAAACTCAGGAGTTAGGTCCAAAAATGGCGGTGGAATCGGTTCAGCGGACGGAGGAGCAGCCACAGGCGGTGGTGTCAAAAGTGGGCCAGCAGGAACGGTCAGATACAAAGAATGTCAAAAGAATCACGCCGTCAGCATAG

Protein sequence

>MsG0380017088.01.T02

MSKESRRQHRWSRRGWMLRVFSCRRRRNTGSGDLCCL*

>MsG0380017088.01.T01

MGSLLRRWHVTTRLETQELGPKMAVESVQRTEEQPQAVVSKVGQQERSDTKNVKRITPSA*