AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004506.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004506.01.T01 MTR_1g081330 97.015 201 6 0 1 201 323 523 4.62e-142 412
MsG0180004506.01.T01 MTR_1g081330 97.015 201 6 0 1 201 408 608 3.34e-141 412
MsG0180004506.01.T01 MTR_7g114680 61.951 205 69 6 1 201 392 591 6.17e-69 224
MsG0180004506.01.T01 MTR_7g114680 61.951 205 69 6 1 201 406 605 8.07e-69 224
MsG0180004506.01.T01 MTR_1g103850 48.507 134 58 4 1 128 401 529 6.38e-29 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004506.01.T01 AT3G04910 40.513 195 88 6 1 191 311 481 2.42e-29 114
MsG0180004506.01.T01 AT3G04910 40.404 198 84 7 1 191 437 607 2.58e-29 115
MsG0180004506.01.T01 AT3G04910 40.404 198 84 7 1 191 414 584 2.82e-29 115
MsG0180004506.01.T01 AT5G28080 47.368 95 27 4 1 95 218 289 1.33e-15 74.3
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 411 482 3.32e-15 74.3
MsG0180004506.01.T01 AT5G28080 47.368 95 27 4 1 95 374 445 3.76e-15 73.9
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 325 396 3.86e-15 73.9
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 325 396 3.86e-15 73.9
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 325 396 3.86e-15 73.9
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 325 396 3.86e-15 73.9
MsG0180004506.01.T01 AT5G28080 46.316 95 28 3 1 95 325 396 3.86e-15 73.9
MsG0180004506.01.T01 AT3G22420 37.838 111 53 4 1 101 459 563 1.28e-11 63.9
MsG0180004506.01.T01 AT3G22420 38.532 109 51 4 1 99 388 490 1.30e-11 63.5
MsG0180004506.01.T01 AT3G22420 38.532 109 51 4 1 99 415 517 1.48e-11 63.5
MsG0180004506.01.T01 AT3G22420 37.838 111 53 4 1 101 486 590 1.50e-11 63.5
MsG0180004506.01.T01 AT3G22420 37.838 111 53 4 1 101 420 524 1.56e-11 63.5
MsG0180004506.01.T01 AT3G22420 37.838 111 53 4 1 101 447 551 1.80e-11 63.5

Find 60 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGATGGCTTGCAGTATC+TGG 0.187825 1:+78964765 MsG0180004506.01.T01:CDS
CCTGGTCCAGATTGCCATTC+AGG 0.193121 1:-78964511 None:intergenic
ACAATATAACGAACGGATAT+TGG 0.236366 1:-78964980 None:intergenic
TGAGCCATCTCAGTTCCTTT+TGG 0.244728 1:-78965033 None:intergenic
GAAACCTTGGTTTGCAAAAC+GGG 0.314429 1:-78964548 None:intergenic
GCTTGCAGTATCTGGGACTA+TGG 0.321875 1:+78964773 MsG0180004506.01.T01:CDS
CTTGGAGAAATTATTGCATT+GGG 0.337257 1:-78964931 None:intergenic
CAAGATACCTAATGGAGTTA+AGG 0.348247 1:+78965059 MsG0180004506.01.T01:CDS
ATCTATCATATCTGCTATAC+TGG 0.356085 1:-78964467 None:intergenic
AGTATAGCAGATATGATAGA+TGG 0.364083 1:+78964469 MsG0180004506.01.T01:CDS
CGGGGAGTTTCAACGATTCC+TGG 0.395128 1:-78964529 None:intergenic
TTGGTACCTGAATGGCAATC+TGG 0.396124 1:+78964505 MsG0180004506.01.T01:CDS
AGATGGGGAAATTGCTTCCT+TGG 0.396509 1:+78964486 MsG0180004506.01.T01:CDS
TAAGCTCCCTTAACTCCATT+AGG 0.407120 1:-78965066 None:intergenic
ACATCCGTGCTTACAACATT+CGG 0.407136 1:-78964653 None:intergenic
AGAAACCTTGGTTTGCAAAA+CGG 0.416271 1:-78964549 None:intergenic
AATGGAGTTAAGGGAGCTTA+AGG 0.421765 1:+78965069 MsG0180004506.01.T01:CDS
AAGATACCTAATGGAGTTAA+GGG 0.431152 1:+78965060 MsG0180004506.01.T01:CDS
TGAGTTCCTGATGAATATCT+TGG 0.433692 1:-78964949 None:intergenic
TCTTGGAGAAATTATTGCAT+TGG 0.442905 1:-78964932 None:intergenic
CACAATTGCGGCAGAAACCT+TGG 0.446587 1:-78964561 None:intergenic
TCGTTATATTGTGGTCTTGA+CGG 0.454758 1:+78964991 MsG0180004506.01.T01:CDS
TACTTCAAGCGGCTCCCTCA+TGG 0.471063 1:+78964594 MsG0180004506.01.T01:CDS
GCTCAAAGCAAGATACCTAA+TGG 0.474760 1:+78965051 MsG0180004506.01.T01:CDS
CATCCGTGCTTACAACATTC+GGG 0.476240 1:-78964652 None:intergenic
CTCAACGCATGGCCGGTTTG+AGG 0.483258 1:+78964678 MsG0180004506.01.T01:CDS
AGAACTTGACATTACAGATC+AGG 0.492936 1:+78964438 MsG0180004506.01.T01:CDS
TTGCTTCCTTGGTACCTGAA+TGG 0.493141 1:+78964497 MsG0180004506.01.T01:CDS
ATTTCTCCAAGATATTCATC+AGG 0.493324 1:+78964943 MsG0180004506.01.T01:CDS
AATATCCGTTCGTTATATTG+TGG 0.497595 1:+78964982 MsG0180004506.01.T01:CDS
GATTGTCATACTCCTCAGAT+TGG 0.497822 1:-78964711 None:intergenic
TACAACATTCGGGAAGTTGA+AGG 0.499709 1:-78964642 None:intergenic
GGAAAGTAATCTCCTCAAAC+CGG 0.502762 1:-78964690 None:intergenic
GCAGATGGCTTGCAGTATCT+GGG 0.518389 1:+78964766 MsG0180004506.01.T01:CDS
CCTGAATGGCAATCTGGACC+AGG 0.529106 1:+78964511 MsG0180004506.01.T01:CDS
GTATAGCAGATATGATAGAT+GGG 0.540214 1:+78964470 MsG0180004506.01.T01:CDS
ACTCTACCGGACTCAATTCA+CGG 0.543079 1:-78964810 None:intergenic
GATGTGCCTCAACGCATGGC+CGG 0.553569 1:+78964671 MsG0180004506.01.T01:CDS
TGAAAGCCGTGAATTGAGTC+CGG 0.554731 1:+78964804 MsG0180004506.01.T01:CDS
CACGGATGTGCCTCAACGCA+TGG 0.557513 1:+78964667 MsG0180004506.01.T01:CDS
GGAGATTACTTTCCAATCTG+AGG 0.559269 1:+78964699 MsG0180004506.01.T01:CDS
GATTATGAAAGAGATCCAAA+AGG 0.561580 1:+78965018 MsG0180004506.01.T01:CDS
GGAGAAATTATTGCATTGGG+GGG 0.567381 1:-78964928 None:intergenic
TCAATATCAGCTGAAGGTCA+AGG 0.570181 1:+78964880 MsG0180004506.01.T01:CDS
CTCAAACCGGCCATGCGTTG+AGG 0.583835 1:-78964677 None:intergenic
TTGGAGAAATTATTGCATTG+GGG 0.587307 1:-78964930 None:intergenic
GATAAATCAATATCAGCTGA+AGG 0.593699 1:+78964874 MsG0180004506.01.T01:CDS
AACATAGCAAACATAGCAGA+TGG 0.594997 1:+78964751 MsG0180004506.01.T01:CDS
CTTTGATCATCAGACTCTAC+CGG 0.597960 1:-78964823 None:intergenic
TGGAGAAATTATTGCATTGG+GGG 0.599277 1:-78964929 None:intergenic
CTTCCCGAATGTTGTAAGCA+CGG 0.607538 1:+78964649 MsG0180004506.01.T01:CDS
AGATCCAAAAGGAACTGAGA+TGG 0.607734 1:+78965029 MsG0180004506.01.T01:CDS
GTGTCGAATCATACTTCAAG+CGG 0.623010 1:+78964583 MsG0180004506.01.T01:CDS
AAACCTTGGTTTGCAAAACG+GGG 0.633543 1:-78964547 None:intergenic
CAAGACCACAATATAACGAA+CGG 0.656535 1:-78964987 None:intergenic
GTGTGAAAGAAAATCCATGA+GGG 0.671483 1:-78964608 None:intergenic
TATAGCAGATATGATAGATG+GGG 0.676482 1:+78964471 MsG0180004506.01.T01:CDS
TATGATTCGACACACAATTG+CGG 0.677561 1:-78964573 None:intergenic
AGATTGCCATTCAGGTACCA+AGG 0.677879 1:-78964503 None:intergenic
TGTGTGAAAGAAAATCCATG+AGG 0.767840 1:-78964609 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGATACCTAATGGAGTTAA+GGG + Chr1:78965060-78965079 MsG0180004506.01.T01:CDS 30.0%
AATATCCGTTCGTTATATTG+TGG + Chr1:78964982-78965001 MsG0180004506.01.T01:CDS 30.0%
ACAATATAACGAACGGATAT+TGG - Chr1:78964983-78965002 None:intergenic 30.0%
AGTATAGCAGATATGATAGA+TGG + Chr1:78964469-78964488 MsG0180004506.01.T01:CDS 30.0%
ATCTATCATATCTGCTATAC+TGG - Chr1:78964470-78964489 None:intergenic 30.0%
ATTTCTCCAAGATATTCATC+AGG + Chr1:78964943-78964962 MsG0180004506.01.T01:CDS 30.0%
CTTGGAGAAATTATTGCATT+GGG - Chr1:78964934-78964953 None:intergenic 30.0%
GATAAATCAATATCAGCTGA+AGG + Chr1:78964874-78964893 MsG0180004506.01.T01:CDS 30.0%
GATTATGAAAGAGATCCAAA+AGG + Chr1:78965018-78965037 MsG0180004506.01.T01:CDS 30.0%
GTATAGCAGATATGATAGAT+GGG + Chr1:78964470-78964489 MsG0180004506.01.T01:CDS 30.0%
TATAGCAGATATGATAGATG+GGG + Chr1:78964471-78964490 MsG0180004506.01.T01:CDS 30.0%
TCTTGGAGAAATTATTGCAT+TGG - Chr1:78964935-78964954 None:intergenic 30.0%
TTGGAGAAATTATTGCATTG+GGG - Chr1:78964933-78964952 None:intergenic 30.0%
! GATTTTCTTTCACACAATCA+GGG + Chr1:78964616-78964635 MsG0180004506.01.T01:CDS 30.0%
AACATAGCAAACATAGCAGA+TGG + Chr1:78964751-78964770 MsG0180004506.01.T01:CDS 35.0%
AGAACTTGACATTACAGATC+AGG + Chr1:78964438-78964457 MsG0180004506.01.T01:CDS 35.0%
CAAGACCACAATATAACGAA+CGG - Chr1:78964990-78965009 None:intergenic 35.0%
CAAGATACCTAATGGAGTTA+AGG + Chr1:78965059-78965078 MsG0180004506.01.T01:CDS 35.0%
GTGTGAAAGAAAATCCATGA+GGG - Chr1:78964611-78964630 None:intergenic 35.0%
TATGATTCGACACACAATTG+CGG - Chr1:78964576-78964595 None:intergenic 35.0%
TGAGTTCCTGATGAATATCT+TGG - Chr1:78964952-78964971 None:intergenic 35.0%
TGGAGAAATTATTGCATTGG+GGG - Chr1:78964932-78964951 None:intergenic 35.0%
TGTGTGAAAGAAAATCCATG+AGG - Chr1:78964612-78964631 None:intergenic 35.0%
! GGATTTTCTTTCACACAATC+AGG + Chr1:78964615-78964634 MsG0180004506.01.T01:CDS 35.0%
! TCGTTATATTGTGGTCTTGA+CGG + Chr1:78964991-78965010 MsG0180004506.01.T01:CDS 35.0%
!! AGAAACCTTGGTTTGCAAAA+CGG - Chr1:78964552-78964571 None:intergenic 35.0%
ACATCCGTGCTTACAACATT+CGG - Chr1:78964656-78964675 None:intergenic 40.0%
AGATCCAAAAGGAACTGAGA+TGG + Chr1:78965029-78965048 MsG0180004506.01.T01:CDS 40.0%
CTTTGATCATCAGACTCTAC+CGG - Chr1:78964826-78964845 None:intergenic 40.0%
GATTGTCATACTCCTCAGAT+TGG - Chr1:78964714-78964733 None:intergenic 40.0%
GCTCAAAGCAAGATACCTAA+TGG + Chr1:78965051-78965070 MsG0180004506.01.T01:CDS 40.0%
GGAAAGTAATCTCCTCAAAC+CGG - Chr1:78964693-78964712 None:intergenic 40.0%
GGAGAAATTATTGCATTGGG+GGG - Chr1:78964931-78964950 None:intergenic 40.0%
GGAGATTACTTTCCAATCTG+AGG + Chr1:78964699-78964718 MsG0180004506.01.T01:CDS 40.0%
GTGTCGAATCATACTTCAAG+CGG + Chr1:78964583-78964602 MsG0180004506.01.T01:CDS 40.0%
TAAGCTCCCTTAACTCCATT+AGG - Chr1:78965069-78965088 None:intergenic 40.0%
TACAACATTCGGGAAGTTGA+AGG - Chr1:78964645-78964664 None:intergenic 40.0%
TCAATATCAGCTGAAGGTCA+AGG + Chr1:78964880-78964899 MsG0180004506.01.T01:CDS 40.0%
!! AAACCTTGGTTTGCAAAACG+GGG - Chr1:78964550-78964569 None:intergenic 40.0%
!! GAAACCTTGGTTTGCAAAAC+GGG - Chr1:78964551-78964570 None:intergenic 40.0%
ACTCTACCGGACTCAATTCA+CGG - Chr1:78964813-78964832 None:intergenic 45.0%
AGATGGGGAAATTGCTTCCT+TGG + Chr1:78964486-78964505 MsG0180004506.01.T01:CDS 45.0%
AGATTGCCATTCAGGTACCA+AGG - Chr1:78964506-78964525 None:intergenic 45.0%
CATCCGTGCTTACAACATTC+GGG - Chr1:78964655-78964674 None:intergenic 45.0%
CTTCCCGAATGTTGTAAGCA+CGG + Chr1:78964649-78964668 MsG0180004506.01.T01:CDS 45.0%
TGAAAGCCGTGAATTGAGTC+CGG + Chr1:78964804-78964823 MsG0180004506.01.T01:CDS 45.0%
TTGCTTCCTTGGTACCTGAA+TGG + Chr1:78964497-78964516 MsG0180004506.01.T01:CDS 45.0%
TTGGTACCTGAATGGCAATC+TGG + Chr1:78964505-78964524 MsG0180004506.01.T01:CDS 45.0%
! TGAGCCATCTCAGTTCCTTT+TGG - Chr1:78965036-78965055 None:intergenic 45.0%
CACAATTGCGGCAGAAACCT+TGG - Chr1:78964564-78964583 None:intergenic 50.0%
! ACTCCCCGTTTTGCAAACCA+AGG + Chr1:78964544-78964563 MsG0180004506.01.T01:CDS 50.0%
! AGCAGATGGCTTGCAGTATC+TGG + Chr1:78964765-78964784 MsG0180004506.01.T01:CDS 50.0%
! GCAGATGGCTTGCAGTATCT+GGG + Chr1:78964766-78964785 MsG0180004506.01.T01:CDS 50.0%
! GCTTGCAGTATCTGGGACTA+TGG + Chr1:78964773-78964792 MsG0180004506.01.T01:CDS 50.0%
CCTGAATGGCAATCTGGACC+AGG + Chr1:78964511-78964530 MsG0180004506.01.T01:CDS 55.0%
CCTGGTCCAGATTGCCATTC+AGG - Chr1:78964514-78964533 None:intergenic 55.0%
CGGGGAGTTTCAACGATTCC+TGG - Chr1:78964532-78964551 None:intergenic 55.0%
TACTTCAAGCGGCTCCCTCA+TGG + Chr1:78964594-78964613 MsG0180004506.01.T01:CDS 55.0%
CACGGATGTGCCTCAACGCA+TGG + Chr1:78964667-78964686 MsG0180004506.01.T01:CDS 60.0%
CTCAAACCGGCCATGCGTTG+AGG - Chr1:78964680-78964699 None:intergenic 60.0%
GATGTGCCTCAACGCATGGC+CGG + Chr1:78964671-78964690 MsG0180004506.01.T01:CDS 60.0%
!! CTCAACGCATGGCCGGTTTG+AGG + Chr1:78964678-78964697 MsG0180004506.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 78964430 78965089 78964430 ID=MsG0180004506.01;Name=MsG0180004506.01
Chr1 mRNA 78964430 78965089 78964430 ID=MsG0180004506.01.T01;Parent=MsG0180004506.01;Name=MsG0180004506.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|219
Chr1 exon 78964430 78965089 78964430 ID=MsG0180004506.01.T01:exon:15260;Parent=MsG0180004506.01.T01
Chr1 CDS 78964430 78965089 78964430 ID=MsG0180004506.01.T01:cds;Parent=MsG0180004506.01.T01
Gene Sequence

>MsG0180004506.01.T01

ATGGTTGCAGAACTTGACATTACAGATCAGGATGTTACCAGTATAGCAGATATGATAGATGGGGAAATTGCTTCCTTGGTACCTGAATGGCAATCTGGACCAGGAATCGTTGAAACTCCCCGTTTTGCAAACCAAGGTTTCTGCCGCAATTGTGTGTCGAATCATACTTCAAGCGGCTCCCTCATGGATTTTCTTTCACACAATCAGGGTAGCCTTCAACTTCCCGAATGTTGTAAGCACGGATGTGCCTCAACGCATGGCCGGTTTGAGGAGATTACTTTCCAATCTGAGGAGTATGACAATCATGTTAGAGAGAATATGAACATAGCAAACATAGCAGATGGCTTGCAGTATCTGGGACTATGGAATCAGCATGAAAGCCGTGAATTGAGTCCGGTAGAGTCTGATGATCAAAGTCATTCTGATGAACAAAATGAACAATTAGATAAATCAATATCAGCTGAAGGTCAAGGATATAATGTTTGTGAAAGTAAGTTTCCCCCCAATGCAATAATTTCTCCAAGATATTCATCAGGAACTCATGATTTTTCCAATATCCGTTCGTTATATTGTGGTCTTGACGGTGATGATTATGAAAGAGATCCAAAAGGAACTGAGATGGCTCAAAGCAAGATACCTAATGGAGTTAAGGGAGCTTAA

Protein sequence

>MsG0180004506.01.T01

MVAELDITDQDVTSIADMIDGEIASLVPEWQSGPGIVETPRFANQGFCRNCVSNHTSSGSLMDFLSHNQGSLQLPECCKHGCASTHGRFEEITFQSEEYDNHVRENMNIANIADGLQYLGLWNQHESRELSPVESDDQSHSDEQNEQLDKSISAEGQGYNVCESKFPPNAIISPRYSSGTHDFSNIRSLYCGLDGDDYERDPKGTEMAQSKIPNGVKGA*