Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047643.01.T01 | XP_003610423.1 | 100 | 55 | 0 | 0 | 1 | 55 | 122 | 176 | 1.73E-31 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047643.01.T01 | G7JJ63 | 100.000 | 55 | 0 | 0 | 1 | 55 | 122 | 176 | 8.26e-32 | 117 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000417.01 | MsG0880047643.01 | 0.807423 | 5.092425e-50 | 2.835280e-47 |
| MsG0180000502.01 | MsG0880047643.01 | 0.805337 | 1.401506e-49 | 7.395375e-47 |
| MsG0180001925.01 | MsG0880047643.01 | 0.803887 | 2.812305e-49 | 1.429749e-46 |
| MsG0880044436.01 | MsG0880047643.01 | 0.839174 | 1.855669e-57 | 2.541731e-54 |
| MsG0180003624.01 | MsG0880047643.01 | 0.806097 | 9.707699e-50 | 5.223333e-47 |
| MsG0180005791.01 | MsG0880047643.01 | 0.812457 | 4.198505e-51 | 2.670999e-48 |
| MsG0180006063.01 | MsG0880047643.01 | 0.805529 | 1.277795e-49 | 6.775769e-47 |
| MsG0780040350.01 | MsG0880047643.01 | 0.807609 | 4.649100e-50 | 2.600903e-47 |
| MsG0780040849.01 | MsG0880047643.01 | 0.806306 | 8.768148e-50 | 4.743171e-47 |
| MsG0780041024.01 | MsG0880047643.01 | 0.817933 | 2.550185e-52 | 1.882553e-49 |
| MsG0780041807.01 | MsG0880047643.01 | 0.864055 | 1.600431e-64 | 4.957226e-61 |
| MsG0280010625.01 | MsG0880047643.01 | 0.813783 | 2.149098e-51 | 1.416607e-48 |
| MsG0380014129.01 | MsG0880047643.01 | 0.815741 | 7.913132e-52 | 5.501674e-49 |
| MsG0380014890.01 | MsG0880047643.01 | 0.813909 | 2.016063e-51 | 1.333447e-48 |
| MsG0680035612.01 | MsG0880047643.01 | 0.807504 | 4.894357e-50 | 2.730845e-47 |
| MsG0480019040.01 | MsG0880047643.01 | 0.821867 | 3.212476e-53 | 2.645485e-50 |
| MsG0480020076.01 | MsG0880047643.01 | 0.810216 | 1.286918e-50 | 7.711816e-48 |
| MsG0480020393.01 | MsG0880047643.01 | 0.800296 | 1.540370e-48 | 7.146863e-46 |
| MsG0480021151.01 | MsG0880047643.01 | 0.847295 | 1.266134e-59 | 2.239840e-56 |
| MsG0480021474.01 | MsG0880047643.01 | 0.821344 | 4.245458e-53 | 3.444884e-50 |
| MsG0480022861.01 | MsG0880047643.01 | 0.828346 | 9.461063e-55 | 9.378313e-52 |
| MsG0480023344.01 | MsG0880047643.01 | 0.846618 | 1.939291e-59 | 3.357424e-56 |
| MsG0480023708.01 | MsG0880047643.01 | 0.829898 | 3.978284e-55 | 4.126968e-52 |
| MsG0580025649.01 | MsG0880047643.01 | 0.807273 | 5.478961e-50 | 3.038681e-47 |
| MsG0380016065.01 | MsG0880047643.01 | 0.818849 | 1.581760e-52 | 1.197579e-49 |
| MsG0380016492.01 | MsG0880047643.01 | 0.863725 | 2.027849e-64 | 6.210834e-61 |
| MsG0380016723.01 | MsG0880047643.01 | 0.819624 | 1.053376e-52 | 8.147823e-50 |
| MsG0780036586.01 | MsG0880047643.01 | 0.824895 | 6.298453e-54 | 5.653368e-51 |
| MsG0780037963.01 | MsG0880047643.01 | 0.823247 | 1.535361e-53 | 1.314819e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047643.01.T01 | MTR_4g132020 | 100.000 | 55 | 0 | 0 | 1 | 55 | 122 | 176 | 2.09e-35 | 117 |
| MsG0880047643.01.T01 | MTR_2g023070 | 89.362 | 47 | 5 | 0 | 9 | 55 | 172 | 218 | 1.05e-24 | 90.9 |
| MsG0880047643.01.T01 | MTR_2g094410 | 72.727 | 44 | 11 | 1 | 10 | 53 | 112 | 154 | 5.66e-17 | 69.7 |
| MsG0880047643.01.T01 | MTR_4g059320 | 68.182 | 44 | 13 | 1 | 10 | 53 | 115 | 157 | 2.67e-15 | 65.5 |
| MsG0880047643.01.T01 | MTR_7g101250 | 66.667 | 45 | 14 | 1 | 10 | 54 | 116 | 159 | 1.11e-14 | 63.9 |
| MsG0880047643.01.T01 | MTR_2g086660 | 63.830 | 47 | 14 | 1 | 10 | 53 | 207 | 253 | 2.42e-14 | 64.3 |
| MsG0880047643.01.T01 | MTR_2g086660 | 63.043 | 46 | 14 | 1 | 10 | 52 | 207 | 252 | 1.85e-13 | 62.4 |
| MsG0880047643.01.T01 | MTR_7g105130 | 65.217 | 46 | 13 | 1 | 11 | 53 | 170 | 215 | 5.82e-13 | 60.1 |
| MsG0880047643.01.T01 | MTR_2g023390 | 56.250 | 48 | 18 | 1 | 9 | 53 | 107 | 154 | 1.36e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047643.01.T01 | AT4G21930 | 72.222 | 54 | 13 | 2 | 4 | 55 | 130 | 183 | 4.93e-18 | 73.2 |
| MsG0880047643.01.T01 | AT1G11700 | 69.811 | 53 | 10 | 2 | 9 | 55 | 149 | 201 | 1.01e-17 | 72.4 |
| MsG0880047643.01.T01 | AT1G61930 | 66.071 | 56 | 12 | 2 | 7 | 55 | 148 | 203 | 1.92e-17 | 72.0 |
| MsG0880047643.01.T01 | AT3G45210 | 61.538 | 52 | 19 | 1 | 2 | 53 | 96 | 146 | 1.36e-15 | 65.9 |
| MsG0880047643.01.T01 | AT4G21970 | 51.786 | 56 | 24 | 1 | 1 | 53 | 88 | 143 | 5.79e-14 | 62.0 |
| MsG0880047643.01.T01 | AT5G03230 | 60.000 | 45 | 17 | 1 | 9 | 53 | 121 | 164 | 1.09e-13 | 61.2 |
| MsG0880047643.01.T01 | AT5G60680 | 65.909 | 44 | 14 | 1 | 10 | 53 | 119 | 161 | 1.62e-13 | 60.8 |
| MsG0880047643.01.T01 | AT3G15040 | 59.184 | 49 | 17 | 1 | 8 | 53 | 193 | 241 | 1.08e-12 | 60.1 |
| MsG0880047643.01.T01 | AT4G04630 | 58.333 | 48 | 17 | 1 | 9 | 53 | 119 | 166 | 1.59e-12 | 58.5 |
| MsG0880047643.01.T01 | AT2G28400 | 60.000 | 45 | 17 | 1 | 10 | 54 | 118 | 161 | 5.66e-11 | 54.3 |
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCCGCCGCATGAGTATT+TGG | 0.311065 | 8:+89125156 | MsG0880047643.01.T01:CDS |
| TTTGGCGCGTGGCCGGAAAA+TGG | 0.357971 | 8:+89125174 | MsG0880047643.01.T01:CDS |
| CCGGAAAATGGCGGCAAATT+CGG | 0.361903 | 8:+89125186 | MsG0880047643.01.T01:CDS |
| GGGGTGGGTCGGACCTTAAA+AGG | 0.389495 | 8:+89125217 | MsG0880047643.01.T01:CDS |
| GACGGGTTCGTGATGCTGTT+TGG | 0.413527 | 8:+89125251 | MsG0880047643.01.T01:CDS |
| CTCAACCATCGAACCCGGTT+TGG | 0.416497 | 8:-89125276 | None:intergenic |
| GATGCTGTTTGGAACCAAAC+CGG | 0.441060 | 8:+89125262 | MsG0880047643.01.T01:CDS |
| ATGAGTATTTGGCGCGTGGC+CGG | 0.490622 | 8:+89125167 | MsG0880047643.01.T01:CDS |
| ATGCTGTTTGGAACCAAACC+GGG | 0.500659 | 8:+89125263 | MsG0880047643.01.T01:CDS |
| AAAAGGCCGTGATTTGAGAC+GGG | 0.530895 | 8:+89125234 | MsG0880047643.01.T01:CDS |
| TATGGATGATGATAACGAAT+CGG | 0.540008 | 8:+89125129 | None:intergenic |
| TGGAACCAAACCGGGTTCGA+TGG | 0.549508 | 8:+89125271 | MsG0880047643.01.T01:CDS |
| TAAAAGGCCGTGATTTGAGA+CGG | 0.552056 | 8:+89125233 | MsG0880047643.01.T01:CDS |
| CACGAACCCGTCTCAAATCA+CGG | 0.568944 | 8:-89125240 | None:intergenic |
| TGATGATAACGAATCGGAGA+TGG | 0.569605 | 8:+89125135 | MsG0880047643.01.T01:CDS |
| CAAACCGGGTTCGATGGTTG+AGG | 0.576026 | 8:+89125277 | MsG0880047643.01.T01:CDS |
| CGCGCCAAATACTCATGCGG+CGG | 0.577795 | 8:-89125160 | None:intergenic |
| TCCACCTCAACCATCGAACC+CGG | 0.586804 | 8:-89125281 | None:intergenic |
| GGCGCGTGGCCGGAAAATGG+CGG | 0.591649 | 8:+89125177 | MsG0880047643.01.T01:CDS |
| CCGCATGAGTATTTGGCGCG+TGG | 0.607155 | 8:+89125163 | MsG0880047643.01.T01:CDS |
| CCACGCGCCAAATACTCATG+CGG | 0.647845 | 8:-89125163 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAAAGGCCGTGATTTGAGA+CGG | + | Chr8:89125233-89125252 | MsG0880047643.01.T01:CDS | 40.0% | |
| ! | TCTCAAATCACGGCCTTTTA+AGG | - | Chr8:89125233-89125252 | None:intergenic | 40.0% |
| ! | TGATGATAACGAATCGGAGA+TGG | + | Chr8:89125135-89125154 | MsG0880047643.01.T01:CDS | 40.0% |
| ! | AAAAGGCCGTGATTTGAGAC+GGG | + | Chr8:89125234-89125253 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | AAATTCGGTTTTCGAAGGGG+TGG | + | Chr8:89125201-89125220 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | AATTCGGTTTTCGAAGGGGT+GGG | + | Chr8:89125202-89125221 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | ATGCTGTTTGGAACCAAACC+GGG | + | Chr8:89125263-89125282 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | CGGCAAATTCGGTTTTCGAA+GGG | + | Chr8:89125197-89125216 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | GATGCTGTTTGGAACCAAAC+CGG | + | Chr8:89125262-89125281 | MsG0880047643.01.T01:CDS | 45.0% |
| ! | GGCAAATTCGGTTTTCGAAG+GGG | + | Chr8:89125198-89125217 | MsG0880047643.01.T01:CDS | 45.0% |
| CACGAACCCGTCTCAAATCA+CGG | - | Chr8:89125243-89125262 | None:intergenic | 50.0% | |
| CCGGAAAATGGCGGCAAATT+CGG | + | Chr8:89125186-89125205 | MsG0880047643.01.T01:CDS | 50.0% | |
| ! | GCGGCAAATTCGGTTTTCGA+AGG | + | Chr8:89125196-89125215 | MsG0880047643.01.T01:CDS | 50.0% |
| !! | CCGAATTTGCCGCCATTTTC+CGG | - | Chr8:89125189-89125208 | None:intergenic | 50.0% |
| ATGAGTATTTGGCGCGTGGC+CGG | + | Chr8:89125167-89125186 | MsG0880047643.01.T01:CDS | 55.0% | |
| CCACGCGCCAAATACTCATG+CGG | - | Chr8:89125166-89125185 | None:intergenic | 55.0% | |
| GGTTCCGCCGCATGAGTATT+TGG | + | Chr8:89125156-89125175 | MsG0880047643.01.T01:CDS | 55.0% | |
| TGGAACCAAACCGGGTTCGA+TGG | + | Chr8:89125271-89125290 | MsG0880047643.01.T01:CDS | 55.0% | |
| ! | GACGGGTTCGTGATGCTGTT+TGG | + | Chr8:89125251-89125270 | MsG0880047643.01.T01:CDS | 55.0% |
| CCGCATGAGTATTTGGCGCG+TGG | + | Chr8:89125163-89125182 | MsG0880047643.01.T01:CDS | 60.0% | |
| CGCGCCAAATACTCATGCGG+CGG | - | Chr8:89125163-89125182 | None:intergenic | 60.0% | |
| GGGGTGGGTCGGACCTTAAA+AGG | + | Chr8:89125217-89125236 | MsG0880047643.01.T01:CDS | 60.0% | |
| TTTGGCGCGTGGCCGGAAAA+TGG | + | Chr8:89125174-89125193 | MsG0880047643.01.T01:CDS | 60.0% | |
| ! | CGGTTTTCGAAGGGGTGGGT+CGG | + | Chr8:89125206-89125225 | MsG0880047643.01.T01:CDS | 60.0% |
| GGCGCGTGGCCGGAAAATGG+CGG | + | Chr8:89125177-89125196 | MsG0880047643.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 89125130 | 89125297 | 89125130 | ID=MsG0880047643.01;Name=MsG0880047643.01 |
| Chr8 | mRNA | 89125130 | 89125297 | 89125130 | ID=MsG0880047643.01.T01;Parent=MsG0880047643.01;Name=MsG0880047643.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|55 |
| Chr8 | exon | 89125130 | 89125297 | 89125130 | ID=MsG0880047643.01.T01:exon:18820;Parent=MsG0880047643.01.T01 |
| Chr8 | CDS | 89125130 | 89125297 | 89125130 | ID=MsG0880047643.01.T01:cds;Parent=MsG0880047643.01.T01 |
| Gene Sequence |
| Protein sequence |
