AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036586.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036586.01.T01 MTR_7g014160 83.482 224 37 0 1 224 232 455 1.89e-135 389
MsG0780036586.01.T01 MTR_7g014610 73.333 225 59 1 1 224 233 457 1.36e-118 346
MsG0780036586.01.T01 MTR_7g014200 69.333 225 66 2 1 224 228 450 1.42e-110 325
MsG0780036586.01.T01 MTR_7g014670 89.697 165 17 0 60 224 77 241 3.35e-107 309
MsG0780036586.01.T01 MTR_7g014620 65.366 205 66 2 24 223 213 417 3.64e-86 262
MsG0780036586.01.T01 MTR_7g013850 49.115 226 108 4 5 224 238 462 3.88e-67 214
MsG0780036586.01.T01 MTR_7g014230 40.404 198 109 5 8 204 232 421 7.06e-36 132
MsG0780036586.01.T01 MTR_7g014630 89.394 66 7 0 1 66 234 299 1.55e-33 124
MsG0780036586.01.T01 MTR_6g015815 34.545 220 129 5 8 224 237 444 3.08e-32 122
MsG0780036586.01.T01 MTR_7g014330 39.130 184 104 4 22 204 243 419 6.45e-32 121
MsG0780036586.01.T01 MTR_6g015315 35.000 220 130 5 8 224 227 436 7.34e-32 120
MsG0780036586.01.T01 MTR_6g015830 34.091 220 129 6 8 224 238 444 6.76e-31 118
MsG0780036586.01.T01 MTR_6g015850 33.333 222 128 6 8 224 235 441 2.30e-30 117
MsG0780036586.01.T01 MTR_7g014250 35.859 198 118 5 8 204 230 419 7.16e-30 115
MsG0780036586.01.T01 MTR_6g015320 35.526 228 117 11 8 224 230 438 2.21e-26 105
MsG0780036586.01.T01 MTR_7g014360 34.574 188 87 5 22 204 193 349 4.03e-25 101
MsG0780036586.01.T01 MTR_3g087640 34.872 195 115 5 22 209 252 441 5.19e-24 99.4
MsG0780036586.01.T01 MTR_2g089735 34.634 205 116 7 13 209 236 430 6.02e-24 99.4
MsG0780036586.01.T01 MTR_8g012750 34.574 188 101 4 25 200 251 428 2.98e-23 97.4
MsG0780036586.01.T01 MTR_3g028210 34.419 215 127 5 18 224 240 448 3.51e-23 97.1
MsG0780036586.01.T01 MTR_7g014310 37.500 144 85 3 62 204 147 286 1.88e-22 93.2
MsG0780036586.01.T01 MTR_2g089670 29.775 178 113 3 23 200 270 435 2.85e-21 91.7
MsG0780036586.01.T01 MTR_2g089765 31.282 195 119 4 23 209 262 449 3.98e-21 91.3
MsG0780036586.01.T01 MTR_2g089755 34.872 195 113 7 22 209 257 444 7.52e-20 87.8
MsG0780036586.01.T01 MTR_2g089650 34.359 195 114 7 22 209 245 432 1.84e-18 83.6
MsG0780036586.01.T01 MTR_7g014205 35.246 122 66 3 84 204 5 114 1.80e-16 73.2
MsG0780036586.01.T01 MTR_7g014210 38.889 108 63 3 99 204 24 130 7.76e-15 70.1
MsG0780036586.01.T01 MTR_7g015860 27.586 203 118 6 25 214 246 432 9.58e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036586.01.T01 AT5G39050 35.841 226 131 8 5 224 234 451 4.84e-34 127
MsG0780036586.01.T01 AT5G39080 33.921 227 133 9 5 224 229 445 1.25e-29 115
MsG0780036586.01.T01 AT3G29670 33.482 224 131 6 5 224 224 433 2.64e-27 108
MsG0780036586.01.T01 AT3G29590 33.333 207 121 6 8 209 221 415 2.74e-26 105
MsG0780036586.01.T01 AT3G29635 30.870 230 139 7 2 224 222 438 2.28e-23 97.8
MsG0780036586.01.T01 AT5G39090 31.982 222 130 12 10 224 224 431 3.00e-22 94.4
MsG0780036586.01.T01 AT5G61160 28.319 226 146 6 4 224 220 434 3.03e-21 91.7
MsG0780036586.01.T01 AT3G29680 28.761 226 141 7 5 224 220 431 7.20e-21 90.5
MsG0780036586.01.T01 AT1G03940 30.769 208 113 7 22 214 245 436 4.39e-20 88.6
MsG0780036586.01.T01 AT3G29690 29.496 139 81 4 92 221 33 163 4.03e-13 65.9

Find 50 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTACCAAATAACTATTT+TGG 0.135502 7:+10135318 MsG0780036586.01.T01:CDS
AGCAAATGGTGTTATCAAAA+TGG 0.235256 7:+10135124 MsG0780036586.01.T01:CDS
GTTATCAAAATGGGAGTTAT+TGG 0.250356 7:+10135134 MsG0780036586.01.T01:CDS
GGTGTTTATGAGATTGATTT+TGG 0.265782 7:+10135552 MsG0780036586.01.T01:CDS
CAATTTCCAAAATAGTTATT+TGG 0.276645 7:-10135324 None:intergenic
AGCAATTCATGGAATTAAAA+TGG 0.285041 7:+10135239 MsG0780036586.01.T01:CDS
TGCTTATGCCCTTGTTTGTT+TGG 0.306724 7:+10135212 MsG0780036586.01.T01:CDS
CGAAGAGAGTCTTCGAGCTT+TGG 0.323617 7:-10135060 None:intergenic
AAGATGTTCCCAAATGAAAA+AGG 0.331198 7:+10135003 None:intergenic
GAGAAAGAGAAGTTTGCATT+TGG 0.340510 7:+10135261 MsG0780036586.01.T01:CDS
GAAACTATCTCCACCTTTGT+TGG 0.379376 7:-10135585 None:intergenic
AGTGAAGGATTTGAAATAAT+AGG 0.395317 7:+10135507 MsG0780036586.01.T01:CDS
AAATTGTGTGTGGGGACATT+TGG 0.398292 7:+10135341 MsG0780036586.01.T01:CDS
TACCATCTTTGCTCTCTGCC+AGG 0.406929 7:-10135631 None:intergenic
TGTTTGGCAAAAGCAATTCA+TGG 0.416726 7:+10135228 MsG0780036586.01.T01:CDS
GTGGCGGGATCTAATAGATT+TGG 0.423530 7:+10135531 MsG0780036586.01.T01:CDS
AATAAAGATGATAAATGAAA+AGG 0.424032 7:+10135434 MsG0780036586.01.T01:CDS
GGTAAAGGTGGAATTGAAGT+TGG 0.427876 7:+10135650 MsG0780036586.01.T01:CDS
GCAAATGGTGTTATCAAAAT+GGG 0.431457 7:+10135125 MsG0780036586.01.T01:CDS
GAGATAGTTTCGGTGGATAG+AGG 0.442745 7:+10135597 MsG0780036586.01.T01:CDS
TTCAATTATTTGTCAAGTGA+AGG 0.447336 7:+10135492 MsG0780036586.01.T01:CDS
ATAAAGATGATAAATGAAAA+GGG 0.447547 7:+10135435 MsG0780036586.01.T01:CDS
AATGAAAAGGGTGTTCTTGA+AGG 0.453937 7:+10135447 MsG0780036586.01.T01:CDS
TTGGATTACATAAATGAAGA+TGG 0.466086 7:+10135378 MsG0780036586.01.T01:CDS
GTAAAGGTGGAATTGAAGTT+GGG 0.475472 7:+10135651 MsG0780036586.01.T01:CDS
GAGAACAAACGATGATAAAG+TGG 0.484849 7:-10135184 None:intergenic
TTTGAACAAGATAAAGCAAA+TGG 0.487133 7:+10135110 MsG0780036586.01.T01:CDS
GTTCTTGAAGGAGCTAGTGA+TGG 0.493087 7:+10135459 MsG0780036586.01.T01:CDS
TTCTTGAAGGAGCTAGTGAT+GGG 0.497785 7:+10135460 MsG0780036586.01.T01:CDS
AACAAAGGTGGAGATAGTTT+CGG 0.498546 7:+10135587 MsG0780036586.01.T01:CDS
TGGATTACATAAATGAAGAT+GGG 0.512542 7:+10135379 MsG0780036586.01.T01:CDS
TTTGGTTGACACAAAACCGT+TGG 0.520534 7:+10135359 MsG0780036586.01.T01:CDS
AAATAGTTATTTGGTAGTGA+TGG 0.521146 7:-10135315 None:intergenic
GGCCTGGCAGAGAGCAAAGA+TGG 0.534800 7:+10135629 MsG0780036586.01.T01:CDS
TAACCTTGCTCTGCAATCTA+CGG 0.549902 7:-10135289 None:intergenic
TCTTCATTTATGTAATCCAA+CGG 0.557148 7:-10135375 None:intergenic
ATAGAGGTTTAACATTGGCC+TGG 0.557519 7:+10135613 MsG0780036586.01.T01:CDS
TTTGAAATAATAGGAGTGGC+GGG 0.565832 7:+10135516 MsG0780036586.01.T01:CDS
ATTTGAAATAATAGGAGTGG+CGG 0.578943 7:+10135515 MsG0780036586.01.T01:CDS
GCAGAGAGCAAAGATGGTAA+AGG 0.579752 7:+10135635 MsG0780036586.01.T01:CDS
TCACCGTAGATTGCAGAGCA+AGG 0.607885 7:+10135286 MsG0780036586.01.T01:CDS
GTGGGGAAGACCAACAAAGG+TGG 0.612550 7:+10135575 MsG0780036586.01.T01:CDS
GAGAGCAAAGATGGTAAAGG+TGG 0.619484 7:+10135638 MsG0780036586.01.T01:CDS
AGGATTTGAAATAATAGGAG+TGG 0.624166 7:+10135512 MsG0780036586.01.T01:CDS
TGGGTGGGGAAGACCAACAA+AGG 0.627130 7:+10135572 MsG0780036586.01.T01:CDS
AAAGGTGGAGATAGTTTCGG+TGG 0.628419 7:+10135590 MsG0780036586.01.T01:CDS
GGATTACATAAATGAAGATG+GGG 0.640685 7:+10135380 MsG0780036586.01.T01:CDS
GGTGGATAGAGGTTTAACAT+TGG 0.647365 7:+10135608 MsG0780036586.01.T01:CDS
ACAAACGATGATAAAGTGGG+TGG 0.683948 7:-10135180 None:intergenic
AGAACAAACGATGATAAAGT+GGG 0.738379 7:-10135183 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGATGATAAATGAAA+AGG + Chr7:10135434-10135453 MsG0780036586.01.T01:CDS 15.0%
!! ATAAAGATGATAAATGAAAA+GGG + Chr7:10135435-10135454 MsG0780036586.01.T01:CDS 15.0%
!! CAATTTCCAAAATAGTTATT+TGG - Chr7:10135327-10135346 None:intergenic 20.0%
! AGCAATTCATGGAATTAAAA+TGG + Chr7:10135239-10135258 MsG0780036586.01.T01:CDS 25.0%
! TCTTCATTTATGTAATCCAA+CGG - Chr7:10135378-10135397 None:intergenic 25.0%
! TGGATTACATAAATGAAGAT+GGG + Chr7:10135379-10135398 MsG0780036586.01.T01:CDS 25.0%
! TTCAATTATTTGTCAAGTGA+AGG + Chr7:10135492-10135511 MsG0780036586.01.T01:CDS 25.0%
! TTGGATTACATAAATGAAGA+TGG + Chr7:10135378-10135397 MsG0780036586.01.T01:CDS 25.0%
! TTTGAACAAGATAAAGCAAA+TGG + Chr7:10135110-10135129 MsG0780036586.01.T01:CDS 25.0%
!! AGTGAAGGATTTGAAATAAT+AGG + Chr7:10135507-10135526 MsG0780036586.01.T01:CDS 25.0%
!! TCACTACCAAATAACTATTT+TGG + Chr7:10135318-10135337 MsG0780036586.01.T01:CDS 25.0%
!!! AAATAGTTATTTGGTAGTGA+TGG - Chr7:10135318-10135337 None:intergenic 25.0%
!!! GTGTTTATGAGATTGATTTT+GGG + Chr7:10135553-10135572 MsG0780036586.01.T01:CDS 25.0%
!!! TTTCGTTTCCTTTTTCATTT+GGG - Chr7:10135014-10135033 None:intergenic 25.0%
AGAACAAACGATGATAAAGT+GGG - Chr7:10135186-10135205 None:intergenic 30.0%
GGATTACATAAATGAAGATG+GGG + Chr7:10135380-10135399 MsG0780036586.01.T01:CDS 30.0%
GTTATCAAAATGGGAGTTAT+TGG + Chr7:10135134-10135153 MsG0780036586.01.T01:CDS 30.0%
! AGGATTTGAAATAATAGGAG+TGG + Chr7:10135512-10135531 MsG0780036586.01.T01:CDS 30.0%
! ATTTGAAATAATAGGAGTGG+CGG + Chr7:10135515-10135534 MsG0780036586.01.T01:CDS 30.0%
!! ACTATTTTGGAAATTGTGTG+TGG + Chr7:10135331-10135350 MsG0780036586.01.T01:CDS 30.0%
!! AGCAAATGGTGTTATCAAAA+TGG + Chr7:10135124-10135143 MsG0780036586.01.T01:CDS 30.0%
!! CTATTTTGGAAATTGTGTGT+GGG + Chr7:10135332-10135351 MsG0780036586.01.T01:CDS 30.0%
!! CTTTCGTTTCCTTTTTCATT+TGG - Chr7:10135015-10135034 None:intergenic 30.0%
!! GCAAATGGTGTTATCAAAAT+GGG + Chr7:10135125-10135144 MsG0780036586.01.T01:CDS 30.0%
!! GGTGTTTATGAGATTGATTT+TGG + Chr7:10135552-10135571 MsG0780036586.01.T01:CDS 30.0%
!! TATTTTGGAAATTGTGTGTG+GGG + Chr7:10135333-10135352 MsG0780036586.01.T01:CDS 30.0%
!! TTATGAGATTGATTTTGGGT+GGG + Chr7:10135557-10135576 MsG0780036586.01.T01:CDS 30.0%
!! TTTATGAGATTGATTTTGGG+TGG + Chr7:10135556-10135575 MsG0780036586.01.T01:CDS 30.0%
AACAAAGGTGGAGATAGTTT+CGG + Chr7:10135587-10135606 MsG0780036586.01.T01:CDS 35.0%
GAGAAAGAGAAGTTTGCATT+TGG + Chr7:10135261-10135280 MsG0780036586.01.T01:CDS 35.0%
GAGAACAAACGATGATAAAG+TGG - Chr7:10135187-10135206 None:intergenic 35.0%
GTAAAGGTGGAATTGAAGTT+GGG + Chr7:10135651-10135670 MsG0780036586.01.T01:CDS 35.0%
TGTTTGGCAAAAGCAATTCA+TGG + Chr7:10135228-10135247 MsG0780036586.01.T01:CDS 35.0%
! TGCTTTTGCCAAACAAACAA+GGG - Chr7:10135223-10135242 None:intergenic 35.0%
! TTTGAAATAATAGGAGTGGC+GGG + Chr7:10135516-10135535 MsG0780036586.01.T01:CDS 35.0%
!! AATGAAAAGGGTGTTCTTGA+AGG + Chr7:10135447-10135466 MsG0780036586.01.T01:CDS 35.0%
!! TATGAGATTGATTTTGGGTG+GGG + Chr7:10135558-10135577 MsG0780036586.01.T01:CDS 35.0%
!! TTGCTTTTGCCAAACAAACA+AGG - Chr7:10135224-10135243 None:intergenic 35.0%
AAATTGTGTGTGGGGACATT+TGG + Chr7:10135341-10135360 MsG0780036586.01.T01:CDS 40.0%
ACAAACGATGATAAAGTGGG+TGG - Chr7:10135183-10135202 None:intergenic 40.0%
ATAGAGGTTTAACATTGGCC+TGG + Chr7:10135613-10135632 MsG0780036586.01.T01:CDS 40.0%
GAAACTATCTCCACCTTTGT+TGG - Chr7:10135588-10135607 None:intergenic 40.0%
GGTAAAGGTGGAATTGAAGT+TGG + Chr7:10135650-10135669 MsG0780036586.01.T01:CDS 40.0%
GGTGGATAGAGGTTTAACAT+TGG + Chr7:10135608-10135627 MsG0780036586.01.T01:CDS 40.0%
TAACCTTGCTCTGCAATCTA+CGG - Chr7:10135292-10135311 None:intergenic 40.0%
TTCTTGAAGGAGCTAGTGAT+GGG + Chr7:10135460-10135479 MsG0780036586.01.T01:CDS 40.0%
TTTGGTTGACACAAAACCGT+TGG + Chr7:10135359-10135378 MsG0780036586.01.T01:CDS 40.0%
! TGCTTATGCCCTTGTTTGTT+TGG + Chr7:10135212-10135231 MsG0780036586.01.T01:CDS 40.0%
AAAGGTGGAGATAGTTTCGG+TGG + Chr7:10135590-10135609 MsG0780036586.01.T01:CDS 45.0%
GAGAGCAAAGATGGTAAAGG+TGG + Chr7:10135638-10135657 MsG0780036586.01.T01:CDS 45.0%
GAGATAGTTTCGGTGGATAG+AGG + Chr7:10135597-10135616 MsG0780036586.01.T01:CDS 45.0%
GCAGAGAGCAAAGATGGTAA+AGG + Chr7:10135635-10135654 MsG0780036586.01.T01:CDS 45.0%
GTTCTTGAAGGAGCTAGTGA+TGG + Chr7:10135459-10135478 MsG0780036586.01.T01:CDS 45.0%
! GTGGCGGGATCTAATAGATT+TGG + Chr7:10135531-10135550 MsG0780036586.01.T01:CDS 45.0%
CGAAGAGAGTCTTCGAGCTT+TGG - Chr7:10135063-10135082 None:intergenic 50.0%
TACCATCTTTGCTCTCTGCC+AGG - Chr7:10135634-10135653 None:intergenic 50.0%
TCACCGTAGATTGCAGAGCA+AGG + Chr7:10135286-10135305 MsG0780036586.01.T01:CDS 50.0%
GTGGGGAAGACCAACAAAGG+TGG + Chr7:10135575-10135594 MsG0780036586.01.T01:CDS 55.0%
TGGGTGGGGAAGACCAACAA+AGG + Chr7:10135572-10135591 MsG0780036586.01.T01:CDS 55.0%
GGCCTGGCAGAGAGCAAAGA+TGG + Chr7:10135629-10135648 MsG0780036586.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 10135006 10135683 10135006 ID=MsG0780036586.01;Name=MsG0780036586.01
Chr7 mRNA 10135006 10135683 10135006 ID=MsG0780036586.01.T01;Parent=MsG0780036586.01;Name=MsG0780036586.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|225
Chr7 exon 10135006 10135683 10135006 ID=MsG0780036586.01.T01:exon:10400;Parent=MsG0780036586.01.T01
Chr7 CDS 10135006 10135683 10135006 ID=MsG0780036586.01.T01:cds;Parent=MsG0780036586.01.T01
Gene Sequence

>MsG0780036586.01.T01

ATGTTCCCAAATGAAAAAGGAAACGAAAGATGCTTGAAGATTTTAGCTTTTGAACCAAAGCTCGAAGACTCTCTTCGTGCTACGTTCAAGCTCACACGTGAAGATTTGAACAAGATAAAGCAAATGGTGTTATCAAAATGGGAGTTATTGGACACAAATGAATTAAATTCTAAGCCACCCACTTTATCATCGTTTGTTCTCACATGTGCTTATGCCCTTGTTTGTTTGGCAAAAGCAATTCATGGAATTAAAATGGAGAAAGAGAAGTTTGCATTTGGATTCACCGTAGATTGCAGAGCAAGGTTAGAGCCATCACTACCAAATAACTATTTTGGAAATTGTGTGTGGGGACATTTGGTTGACACAAAACCGTTGGATTACATAAATGAAGATGGGGTGTTTTTAGTTGCTAAGTGTATTCATGAGAAAATAAAGATGATAAATGAAAAGGGTGTTCTTGAAGGAGCTAGTGATGGGTTTAGTAAATTCAATTATTTGTCAAGTGAAGGATTTGAAATAATAGGAGTGGCGGGATCTAATAGATTTGGTGTTTATGAGATTGATTTTGGGTGGGGAAGACCAACAAAGGTGGAGATAGTTTCGGTGGATAGAGGTTTAACATTGGCCTGGCAGAGAGCAAAGATGGTAAAGGTGGAATTGAAGTTGGGCTTGTTCTAG

Protein sequence

>MsG0780036586.01.T01

MFPNEKGNERCLKILAFEPKLEDSLRATFKLTREDLNKIKQMVLSKWELLDTNELNSKPPTLSSFVLTCAYALVCLAKAIHGIKMEKEKFAFGFTVDCRARLEPSLPNNYFGNCVWGHLVDTKPLDYINEDGVFLVAKCIHEKIKMINEKGVLEGASDGFSKFNYLSSEGFEIIGVAGSNRFGVYEIDFGWGRPTKVEIVSVDRGLTLAWQRAKMVKVELKLGLF*