AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029218.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029218.01.T01 MTR_5g082940 95.238 126 5 1 1 125 1 126 9.64e-74 217
MsG0580029218.01.T01 MTR_3g071300 47.794 136 53 4 1 125 1 129 1.43e-35 120
MsG0580029218.01.T01 MTR_3g094000 44.068 59 33 0 72 130 89 147 1.52e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029218.01.T01 AT3G11110 32.727 110 71 2 18 125 22 130 4.35e-11 57.4
MsG0580029218.01.T01 AT2G17450 30.894 123 71 4 15 130 15 130 6.73e-11 57.4

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGGGGAGTGTGGTGGTTT+TGG 0.221415 5:+93868996 None:intergenic
AGTGGTTCGTCTTCGGGTTC+GGG 0.262925 5:+93869112 None:intergenic
CAATGCATGGTTTGAAGTAT+TGG 0.271086 5:+93868886 None:intergenic
GAGTGGTTCGTCTTCGGGTT+CGG 0.280107 5:+93869111 None:intergenic
ACTTGGAGTGGTTCGTCTTC+GGG 0.286558 5:+93869106 None:intergenic
GACTTGGAGTGGTTCGTCTT+CGG 0.310812 5:+93869105 None:intergenic
CACTCTTCCTCTGTGCCCGC+TGG 0.403523 5:-93869024 MsG0580029218.01.T01:CDS
TTGGATTGGTGTAGTATGAT+AGG 0.417548 5:+93868905 None:intergenic
GGCGTTGGCGTCGAGTCCTT+CGG 0.420894 5:+93868940 None:intergenic
TATGATAGGAAGCTTCTTGA+TGG 0.447186 5:+93868919 None:intergenic
GGAGTTTGTGGCCTTTGACT+TGG 0.470416 5:+93869089 None:intergenic
AAACCATGCATTGGAGGAAA+CGG 0.503937 5:-93868876 MsG0580029218.01.T01:CDS
AGGAAGCTTCTTGATGGCGT+TGG 0.511777 5:+93868925 None:intergenic
TACTTCAAACCATGCATTGG+AGG 0.519213 5:-93868882 MsG0580029218.01.T01:CDS
CAATACTTCAAACCATGCAT+TGG 0.523711 5:-93868885 MsG0580029218.01.T01:CDS
CAATGAAGAAGAGGAGTTTG+TGG 0.523900 5:+93869077 None:intergenic
ATTGTGAATGTGTTGATGCA+TGG 0.528588 5:-93868779 MsG0580029218.01.T01:CDS
CATGGTTTGAAGTATTGGAT+TGG 0.530983 5:+93868891 None:intergenic
GAGGATGATGATGGTGAAGA+TGG 0.534987 5:+93869054 None:intergenic
TGTTTAAGCACGTTTAGAGA+TGG 0.546859 5:-93868842 MsG0580029218.01.T01:CDS
GAGATGGCGCGTGGGGAGTG+TGG 0.554524 5:+93868987 None:intergenic
ATTCCGTTTCCTCCAATGCA+TGG 0.554845 5:+93868873 None:intergenic
TTGTGGCCTTTGACTTGGAG+TGG 0.557650 5:+93869094 None:intergenic
TGAAGATGGCAATGAAGAAG+AGG 0.560013 5:+93869068 None:intergenic
GTGGTTCGTCTTCGGGTTCG+GGG 0.568042 5:+93869113 None:intergenic
TGGAGGTGTGAGATGGCGCG+TGG 0.572879 5:+93868978 None:intergenic
CTAACATCAAAACATTCGAC+TGG 0.578650 5:-93869144 MsG0580029218.01.T01:CDS
GGAGGTGTGAGATGGCGCGT+GGG 0.578793 5:+93868979 None:intergenic
AGAGGAAGAGTGCTGTGAAG+AGG 0.584195 5:+93869035 None:intergenic
GACGAACCACTCCAAGTCAA+AGG 0.593233 5:-93869100 MsG0580029218.01.T01:CDS
ATGGCGCGTGGGGAGTGTGG+TGG 0.607095 5:+93868990 None:intergenic
GAGGTGTGAGATGGCGCGTG+GGG 0.607748 5:+93868980 None:intergenic
CACGTTTAGAGATGGTGAGA+AGG 0.608612 5:-93868834 MsG0580029218.01.T01:CDS
TGCTGTGAAGAGGATGATGA+TGG 0.611187 5:+93869045 None:intergenic
ACATTCTTCTCTCAATCCGA+AGG 0.611520 5:-93868956 MsG0580029218.01.T01:CDS
TTTGATGTTAGGGTTTGACA+TGG 0.641304 5:+93869156 None:intergenic
GGAAGAGCCAGCGGGCACAG+AGG 0.642468 5:+93869017 None:intergenic
AAGAATGTGGAGGTGTGAGA+TGG 0.658890 5:+93868971 None:intergenic
TTCGGATTGAGAGAAGAATG+TGG 0.662383 5:+93868958 None:intergenic
ATGAAATAATGATCACAACT+CGG 0.680055 5:+93868800 None:intergenic
GGATTGAGAGAAGAATGTGG+AGG 0.769620 5:+93868961 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGAAATAATGATCACAACT+CGG + Chr5:93869129-93869148 None:intergenic 25.0%
!!! AGTCGAATGTTTTGATGTTA+GGG + Chr5:93868783-93868802 None:intergenic 30.0%
CAATACTTCAAACCATGCAT+TGG - Chr5:93869041-93869060 MsG0580029218.01.T01:CDS 35.0%
CTAACATCAAAACATTCGAC+TGG - Chr5:93868782-93868801 MsG0580029218.01.T01:CDS 35.0%
TATGATAGGAAGCTTCTTGA+TGG + Chr5:93869010-93869029 None:intergenic 35.0%
TGTTTAAGCACGTTTAGAGA+TGG - Chr5:93869084-93869103 MsG0580029218.01.T01:CDS 35.0%
! ATTGTGAATGTGTTGATGCA+TGG - Chr5:93869147-93869166 MsG0580029218.01.T01:CDS 35.0%
! CAATGCATGGTTTGAAGTAT+TGG + Chr5:93869043-93869062 None:intergenic 35.0%
!! CATGGTTTGAAGTATTGGAT+TGG + Chr5:93869038-93869057 None:intergenic 35.0%
!! TTGGATTGGTGTAGTATGAT+AGG + Chr5:93869024-93869043 None:intergenic 35.0%
!!! CAGTCGAATGTTTTGATGTT+AGG + Chr5:93868784-93868803 None:intergenic 35.0%
AAACCATGCATTGGAGGAAA+CGG - Chr5:93869050-93869069 MsG0580029218.01.T01:CDS 40.0%
ACATTCTTCTCTCAATCCGA+AGG - Chr5:93868970-93868989 MsG0580029218.01.T01:CDS 40.0%
CAATGAAGAAGAGGAGTTTG+TGG + Chr5:93868852-93868871 None:intergenic 40.0%
TACTTCAAACCATGCATTGG+AGG - Chr5:93869044-93869063 MsG0580029218.01.T01:CDS 40.0%
TGAAGATGGCAATGAAGAAG+AGG + Chr5:93868861-93868880 None:intergenic 40.0%
TTCGGATTGAGAGAAGAATG+TGG + Chr5:93868971-93868990 None:intergenic 40.0%
ATTCCGTTTCCTCCAATGCA+TGG + Chr5:93869056-93869075 None:intergenic 45.0%
CACGTTTAGAGATGGTGAGA+AGG - Chr5:93869092-93869111 MsG0580029218.01.T01:CDS 45.0%
GGATTGAGAGAAGAATGTGG+AGG + Chr5:93868968-93868987 None:intergenic 45.0%
TGCTGTGAAGAGGATGATGA+TGG + Chr5:93868884-93868903 None:intergenic 45.0%
! AAGAATGTGGAGGTGTGAGA+TGG + Chr5:93868958-93868977 None:intergenic 45.0%
!! GAGGATGATGATGGTGAAGA+TGG + Chr5:93868875-93868894 None:intergenic 45.0%
ACTTGGAGTGGTTCGTCTTC+GGG + Chr5:93868823-93868842 None:intergenic 50.0%
GACGAACCACTCCAAGTCAA+AGG - Chr5:93868826-93868845 MsG0580029218.01.T01:CDS 50.0%
GACTTGGAGTGGTTCGTCTT+CGG + Chr5:93868824-93868843 None:intergenic 50.0%
GGAGTTTGTGGCCTTTGACT+TGG + Chr5:93868840-93868859 None:intergenic 50.0%
!! AGAGGAAGAGTGCTGTGAAG+AGG + Chr5:93868894-93868913 None:intergenic 50.0%
!! AGGAAGCTTCTTGATGGCGT+TGG + Chr5:93869004-93869023 None:intergenic 50.0%
!! TTGTGGCCTTTGACTTGGAG+TGG + Chr5:93868835-93868854 None:intergenic 50.0%
! AGTGGTTCGTCTTCGGGTTC+GGG + Chr5:93868817-93868836 None:intergenic 55.0%
! GAGTGGTTCGTCTTCGGGTT+CGG + Chr5:93868818-93868837 None:intergenic 55.0%
!!! GGTGGTTTTGGAAGAGCCAG+CGG + Chr5:93868921-93868940 None:intergenic 55.0%
!!! GTGGTTTTGGAAGAGCCAGC+GGG + Chr5:93868920-93868939 None:intergenic 55.0%
! GTGGTTCGTCTTCGGGTTCG+GGG + Chr5:93868816-93868835 None:intergenic 60.0%
!! CGTGGGGAGTGTGGTGGTTT+TGG + Chr5:93868933-93868952 None:intergenic 60.0%
CACTCTTCCTCTGTGCCCGC+TGG - Chr5:93868902-93868921 MsG0580029218.01.T01:CDS 65.0%
GAGGTGTGAGATGGCGCGTG+GGG + Chr5:93868949-93868968 None:intergenic 65.0%
! GGAGGTGTGAGATGGCGCGT+GGG + Chr5:93868950-93868969 None:intergenic 65.0%
! TGGAGGTGTGAGATGGCGCG+TGG + Chr5:93868951-93868970 None:intergenic 65.0%
!! GGCGTTGGCGTCGAGTCCTT+CGG + Chr5:93868989-93869008 None:intergenic 65.0%
GAGATGGCGCGTGGGGAGTG+TGG + Chr5:93868942-93868961 None:intergenic 70.0%
GGAAGAGCCAGCGGGCACAG+AGG + Chr5:93868912-93868931 None:intergenic 70.0%
! ATGGCGCGTGGGGAGTGTGG+TGG + Chr5:93868939-93868958 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93868772 93869176 93868772 ID=MsG0580029218.01;Name=MsG0580029218.01
Chr5 mRNA 93868772 93869176 93868772 ID=MsG0580029218.01.T01;Parent=MsG0580029218.01;Name=MsG0580029218.01.T01;_AED=0.13;_eAED=0.16;_QI=0|-1|0|1|-1|0|1|0|134
Chr5 exon 93868772 93869176 93868772 ID=MsG0580029218.01.T01:exon:18189;Parent=MsG0580029218.01.T01
Chr5 CDS 93868772 93869176 93868772 ID=MsG0580029218.01.T01:cds;Parent=MsG0580029218.01.T01
Chr5 mRNA 93868858 93868893 93868858 ID=MsG0580029218.01.T02;Parent=MsG0580029218.01;Name=MsG0580029218.01.T02;_AED=0.47;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|11
Chr5 exon 93868858 93868893 93868858 ID=MsG0580029218.01.T02:exon:18190;Parent=MsG0580029218.01.T02
Chr5 CDS 93868858 93868893 93868858 ID=MsG0580029218.01.T02:cds;Parent=MsG0580029218.01.T02
Gene Sequence

>MsG0580029218.01.T01

ATGTCAAACCCTAACATCAAAACATTCGACTGGACGTTCAACCCCGAACCCGAAGACGAACCACTCCAAGTCAAAGGCCACAAACTCCTCTTCTTCATTGCCATCTTCACCATCATCATCCTCTTCACAGCACTCTTCCTCTGTGCCCGCTGGCTCTTCCAAAACCACCACACTCCCCACGCGCCATCTCACACCTCCACATTCTTCTCTCAATCCGAAGGACTCGACGCCAACGCCATCAAGAAGCTTCCTATCATACTACACCAATCCAATACTTCAAACCATGCATTGGAGGAAACGGAATGTTGCATTTGTTTAAGCACGTTTAGAGATGGTGAGAAGGTGAAAGTGTTACCGAGTTGTGATCATTATTTCATTGTGAATGTGTTGATGCATGGCTTGTGA

>MsG0580029218.01.T02

ATGCATTGGAGGAAACGGAATGTTGCATTTGTTTAA

Protein sequence

>MsG0580029218.01.T01

MSNPNIKTFDWTFNPEPEDEPLQVKGHKLLFFIAIFTIIILFTALFLCARWLFQNHHTPHAPSHTSTFFSQSEGLDANAIKKLPIILHQSNTSNHALEETECCICLSTFRDGEKVKVLPSCDHYFIVNVLMHGL*

>MsG0580029218.01.T02

MHWRKRNVAFV*