Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001004.01.T01 | AFK37642.1 | 78.667 | 75 | 3 | 1 | 1 | 75 | 1 | 62 | 2.49E-21 | 89 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001004.01.T01 | O82337 | 52.381 | 63 | 15 | 2 | 13 | 75 | 15 | 62 | 5.37E-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001004.01.T01 | I3SBK0 | 78.667 | 75 | 3 | 1 | 1 | 75 | 1 | 62 | 1.19e-21 | 89.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000028.01 | MsG0180001004.01 | 0.829889 | 3.997174e-55 | 4.145546e-52 |
| MsG0180000466.01 | MsG0180001004.01 | 0.801656 | 8.124733e-49 | 3.901335e-46 |
| MsG0180001004.01 | MsG0180001029.01 | 0.809106 | 2.229640e-50 | 1.297554e-47 |
| MsG0180001004.01 | MsG0180002238.01 | 0.804060 | 2.588930e-49 | 1.322044e-46 |
| MsG0180001004.01 | MsG0180003320.01 | 0.804334 | 2.270183e-49 | 1.167398e-46 |
| MsG0180001004.01 | MsG0180004467.01 | 0.800740 | 1.250657e-48 | 5.868180e-46 |
| MsG0180001004.01 | MsG0180005267.01 | 0.823402 | 1.411894e-53 | 1.214332e-50 |
| MsG0180001004.01 | MsG0180006183.01 | 0.801240 | 9.882907e-49 | 4.696415e-46 |
| MsG0180001004.01 | MsG0280007505.01 | 0.806886 | 6.616637e-50 | 3.632875e-47 |
| MsG0180001004.01 | MsG0280007653.01 | -0.801726 | 7.859670e-49 | 3.780763e-46 |
| MsG0180001004.01 | MsG0280009432.01 | 0.802680 | 5.000603e-49 | 2.464676e-46 |
| MsG0180001004.01 | MsG0280009526.01 | 0.800285 | 1.548521e-48 | 7.182555e-46 |
| MsG0180001004.01 | MsG0280010617.01 | 0.801881 | 7.302596e-49 | 3.527027e-46 |
| MsG0180001004.01 | MsG0380016031.01 | 0.816430 | 5.552041e-52 | 3.933038e-49 |
| MsG0180001004.01 | MsG0380016490.01 | 0.817038 | 4.057446e-52 | 2.922688e-49 |
| MsG0180001004.01 | MsG0380017168.01 | 0.805422 | 1.345380e-49 | 7.114785e-47 |
| MsG0180001004.01 | MsG0480018362.01 | 0.807348 | 5.281803e-50 | 2.935001e-47 |
| MsG0180001004.01 | MsG0480019171.01 | 0.828552 | 8.438353e-55 | 8.415360e-52 |
| MsG0180001004.01 | MsG0480020810.01 | 0.816997 | 4.143021e-52 | 2.981185e-49 |
| MsG0180001004.01 | MsG0480020904.01 | 0.830873 | 2.297965e-55 | 2.452299e-52 |
| MsG0180001004.01 | MsG0580024980.01 | 0.827585 | 1.442575e-54 | 1.398476e-51 |
| MsG0180001004.01 | MsG0580026180.01 | 0.814014 | 1.910943e-51 | 1.267585e-48 |
| MsG0180001004.01 | MsG0580029158.01 | 0.812523 | 4.061022e-51 | 2.587989e-48 |
| MsG0180001004.01 | MsG0580029218.01 | 0.820939 | 5.262478e-53 | 4.222491e-50 |
| MsG0180001004.01 | MsG0680031200.01 | 0.807193 | 5.696153e-50 | 3.152538e-47 |
| MsG0180001004.01 | MsG0780035930.01 | 0.806644 | 7.442069e-50 | 4.060423e-47 |
| MsG0180001004.01 | MsG0780038657.01 | 0.809563 | 1.778718e-50 | 1.047834e-47 |
| MsG0180001004.01 | MsG0780040716.01 | 0.816251 | 6.087688e-52 | 4.291821e-49 |
| MsG0180001004.01 | MsG0880043550.01 | 0.807687 | 4.474413e-50 | 2.508369e-47 |
| MsG0180001004.01 | MsG0880045725.01 | 0.821355 | 4.220359e-53 | 3.425516e-50 |
| MsG0180001004.01 | MsG0880046010.01 | -0.802447 | 5.587377e-49 | 2.737586e-46 |
| MsG0180001004.01 | MsG0880046014.01 | 0.811487 | 6.832449e-51 | 4.234882e-48 |
| MsG0180001004.01 | MsG0880046080.01 | 0.811407 | 7.109410e-51 | 4.397343e-48 |
| MsG0180001004.01 | MsG0880046870.01 | 0.808668 | 2.765762e-50 | 1.590866e-47 |
| MsG0180001004.01 | MsG0880047090.01 | 0.800532 | 1.379200e-48 | 6.437109e-46 |
| MsG0180001004.01 | MsG0880047450.01 | 0.808865 | 2.510170e-50 | 1.451399e-47 |
| MsG0180001004.01 | MsG0880047704.01 | 0.801003 | 1.104961e-48 | 5.219794e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001004.01.T01 | MTR_1g028610 | 78.667 | 75 | 3 | 1 | 1 | 75 | 1 | 62 | 8.44e-25 | 87.8 |
| MsG0180001004.01.T01 | MTR_3g090830 | 63.889 | 72 | 11 | 2 | 4 | 75 | 7 | 63 | 7.70e-21 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001004.01.T01 | AT2G46330 | 52.381 | 63 | 15 | 2 | 13 | 75 | 15 | 62 | 5.47e-12 | 55.8 |
| MsG0180001004.01.T01 | AT3G61640 | 45.833 | 72 | 24 | 2 | 4 | 75 | 6 | 62 | 1.19e-11 | 54.7 |
| MsG0180001004.01.T01 | AT5G53250 | 57.692 | 52 | 9 | 1 | 24 | 75 | 25 | 63 | 6.63e-11 | 52.8 |
Find 15 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGTGGGAGCGGGAGC+AGG | 0.433526 | 1:+14390734 | None:intergenic |
| GAAGCAAGGCCACCAGCATT+AGG | 0.444107 | 1:+14389939 | None:intergenic |
| GTGGGAGCGGGAGCAGGAGC+CGG | 0.473296 | 1:+14390740 | None:intergenic |
| TTCTTCACAGTCTGTTGCTC+CGG | 0.477404 | 1:-14390759 | MsG0180001004.01.T01:CDS |
| TTAGGACATATGCTATTGCT+TGG | 0.507960 | 1:+14389957 | None:intergenic |
| TTGACAGTTCACAAACAGTT+CGG | 0.509126 | 1:-14390134 | MsG0180001004.01.T01:intron |
| CATACCATCACTTGTGGGAG+CGG | 0.554416 | 1:+14390727 | None:intergenic |
| ATACCATCACTTGTGGGAGC+GGG | 0.556858 | 1:+14390728 | None:intergenic |
| GAAAACATACCATCACTTGT+GGG | 0.559175 | 1:+14390722 | None:intergenic |
| TGAAAACATACCATCACTTG+TGG | 0.566489 | 1:+14390721 | None:intergenic |
| GCTCCCGCTCCCACAAGTGA+TGG | 0.569380 | 1:-14390731 | MsG0180001004.01.T01:CDS |
| AATAGCATATGTCCTAATGC+TGG | 0.576700 | 1:-14389951 | MsG0180001004.01.T01:CDS |
| AGCATATGTCCTAATGCTGG+TGG | 0.610729 | 1:-14389948 | MsG0180001004.01.T01:CDS |
| AAGATGCATAAATAAGACCA+AGG | 0.662451 | 1:+14390795 | None:intergenic |
| AATGATGTAAGTGAGAAGCA+AGG | 0.690125 | 1:+14389925 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATCAAAAAATTTAAATA+GGG | - | Chr1:14390289-14390308 | MsG0180001004.01.T01:intron | 10.0% |
| !! | CTCAATCAAAAAATTTAAAT+AGG | - | Chr1:14390288-14390307 | MsG0180001004.01.T01:intron | 15.0% |
| !! | TAAAAAACACATGAACTAAA+CGG | - | Chr1:14390550-14390569 | MsG0180001004.01.T01:intron | 20.0% |
| !! | TAATTGATATAGACTATCTT+GGG | - | Chr1:14390492-14390511 | MsG0180001004.01.T01:intron | 20.0% |
| !!! | CATCACAATTTTTGATATTT+TGG | + | Chr1:14390417-14390436 | None:intergenic | 20.0% |
| !!! | TATCACAGTTTGATATTTTT+GGG | - | Chr1:14390655-14390674 | MsG0180001004.01.T01:intron | 20.0% |
| ! | AACAACAATATTGGATAGAA+CGG | + | Chr1:14390703-14390722 | None:intergenic | 25.0% |
| ! | AATAACAGTTCCATTCTTAT+CGG | + | Chr1:14390591-14390610 | None:intergenic | 25.0% |
| ! | CAAGATAGTCTATATCAATT+AGG | + | Chr1:14390493-14390512 | None:intergenic | 25.0% |
| ! | CTAATTGATATAGACTATCT+TGG | - | Chr1:14390491-14390510 | MsG0180001004.01.T01:intron | 25.0% |
| ! | TCAAGAGAAATAACACAATT+AGG | + | Chr1:14390144-14390163 | None:intergenic | 25.0% |
| !! | AACTGTTCTTAAAATTTTGC+AGG | - | Chr1:14390751-14390770 | MsG0180001004.01.T01:CDS | 25.0% |
| !! | CTATCACAGTTTGATATTTT+TGG | - | Chr1:14390654-14390673 | MsG0180001004.01.T01:intron | 25.0% |
| !! | TTCTATCCAATATTGTTGTT+TGG | - | Chr1:14390703-14390722 | MsG0180001004.01.T01:intron | 25.0% |
| !!! | ATATTTTTGGGTTTAGTCTT+TGG | - | Chr1:14390667-14390686 | MsG0180001004.01.T01:intron | 25.0% |
| !!! | TTTGATATTTTGGAGAATCA+CGG | + | Chr1:14390407-14390426 | None:intergenic | 25.0% |
| AAGATGCATAAATAAGACCA+AGG | + | Chr1:14389951-14389970 | None:intergenic | 30.0% | |
| CAAAAAAGTTTCTCAACATG+AGG | + | Chr1:14390253-14390272 | None:intergenic | 30.0% | |
| CATCCATGAATCATATTAGA+TGG | + | Chr1:14390116-14390135 | None:intergenic | 30.0% | |
| GCAATTTAGACTACAAAATC+TGG | - | Chr1:14390321-14390340 | MsG0180001004.01.T01:intron | 30.0% | |
| TCACCATCTAATATGATTCA+TGG | - | Chr1:14390110-14390129 | MsG0180001004.01.T01:intron | 30.0% | |
| TTGCAACCAAACAACAATAT+TGG | + | Chr1:14390712-14390731 | None:intergenic | 30.0% | |
| AATAGCATATGTCCTAATGC+TGG | - | Chr1:14390792-14390811 | MsG0180001004.01.T01:CDS | 35.0% | |
| AATGATGTAAGTGAGAAGCA+AGG | + | Chr1:14390821-14390840 | None:intergenic | 35.0% | |
| CTTCAACTACAATCACATTG+TGG | + | Chr1:14390365-14390384 | None:intergenic | 35.0% | |
| GAAAACATACCATCACTTGT+GGG | + | Chr1:14390024-14390043 | None:intergenic | 35.0% | |
| TGAAAACATACCATCACTTG+TGG | + | Chr1:14390025-14390044 | None:intergenic | 35.0% | |
| TTAGGACATATGCTATTGCT+TGG | + | Chr1:14390789-14390808 | None:intergenic | 35.0% | |
| TTGACAGTTCACAAACAGTT+CGG | - | Chr1:14390609-14390628 | MsG0180001004.01.T01:intron | 35.0% | |
| ! | GTCTAAAACACTGCATGTTT+AGG | - | Chr1:14390522-14390541 | MsG0180001004.01.T01:intron | 35.0% |
| ! | TTATGCATCTTTTGCGTCAA+TGG | - | Chr1:14389960-14389979 | MsG0180001004.01.T01:CDS | 35.0% |
| TTGTTTGGTTGCAAAACGCA+TGG | - | Chr1:14390718-14390737 | MsG0180001004.01.T01:intron | 40.0% | |
| AGCATATGTCCTAATGCTGG+TGG | - | Chr1:14390795-14390814 | MsG0180001004.01.T01:CDS | 45.0% | |
| GAAGTCGCACCAATCATGTA+TGG | - | Chr1:14390381-14390400 | MsG0180001004.01.T01:intron | 45.0% | |
| GAATCACGGCCATACATGAT+TGG | + | Chr1:14390393-14390412 | None:intergenic | 45.0% | |
| TTCTTCACAGTCTGTTGCTC+CGG | - | Chr1:14389984-14390003 | MsG0180001004.01.T01:intron | 45.0% | |
| ATACCATCACTTGTGGGAGC+GGG | + | Chr1:14390018-14390037 | None:intergenic | 50.0% | |
| CAGTTCCATTCTTATCGGCG+CGG | + | Chr1:14390586-14390605 | None:intergenic | 50.0% | |
| CATACCATCACTTGTGGGAG+CGG | + | Chr1:14390019-14390038 | None:intergenic | 50.0% | |
| ! | TTGAACCGCGCCGATAAGAA+TGG | - | Chr1:14390578-14390597 | MsG0180001004.01.T01:intron | 50.0% |
| !! | TTTTGGGTGGTCGTGTTCCT+TGG | - | Chr1:14389931-14389950 | MsG0180001004.01.T01:CDS | 50.0% |
| GAAGCAAGGCCACCAGCATT+AGG | + | Chr1:14390807-14390826 | None:intergenic | 55.0% | |
| GCTCCCGCTCCCACAAGTGA+TGG | - | Chr1:14390012-14390031 | MsG0180001004.01.T01:intron | 65.0% | |
| TCACTTGTGGGAGCGGGAGC+AGG | + | Chr1:14390012-14390031 | None:intergenic | 65.0% | |
| GTGGGAGCGGGAGCAGGAGC+CGG | + | Chr1:14390006-14390025 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 14389919 | 14390846 | 14389919 | ID=MsG0180001004.01;Name=MsG0180001004.01 |
| Chr1 | mRNA | 14389919 | 14390846 | 14389919 | ID=MsG0180001004.01.T01;Parent=MsG0180001004.01;Name=MsG0180001004.01.T01;_AED=0.48;_eAED=0.57;_QI=0|0|0|1|0|0|3|0|75 |
| Chr1 | exon | 14390728 | 14390846 | 14390728 | ID=MsG0180001004.01.T01:exon:9821;Parent=MsG0180001004.01.T01 |
| Chr1 | exon | 14390135 | 14390169 | 14390135 | ID=MsG0180001004.01.T01:exon:9820;Parent=MsG0180001004.01.T01 |
| Chr1 | exon | 14389919 | 14389992 | 14389919 | ID=MsG0180001004.01.T01:exon:9819;Parent=MsG0180001004.01.T01 |
| Chr1 | CDS | 14390728 | 14390846 | 14390728 | ID=MsG0180001004.01.T01:cds;Parent=MsG0180001004.01.T01 |
| Chr1 | CDS | 14390135 | 14390169 | 14390135 | ID=MsG0180001004.01.T01:cds;Parent=MsG0180001004.01.T01 |
| Chr1 | CDS | 14389919 | 14389992 | 14389919 | ID=MsG0180001004.01.T01:cds;Parent=MsG0180001004.01.T01 |
| Gene Sequence |
| Protein sequence |
