AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0034770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0034770 MtrunA17_Chr1g0197011 94.118 153 8 1 3 154 4 156 6.83e-99 281
Msa0034770 MtrunA17_Chr1g0177041 51.163 86 38 2 69 153 69 151 2.10e-26 97.8
Msa0034770 MtrunA17_Chr1g0197031 36.025 161 86 6 1 154 1 151 5.11e-25 94.4
Msa0034770 MtrunA17_Chr1g0197021 34.211 152 86 6 1 148 1 142 3.47e-21 84.3
Msa0034770 MtrunA17_Chr3g0081611 29.299 157 98 4 7 153 3 156 2.12e-19 80.1
Msa0034770 MtrunA17_Chr5g0426881 33.333 150 88 6 10 154 50 192 7.61e-19 81.6
Msa0034770 MtrunA17_Chr1g0196991 73.171 41 11 0 109 149 120 160 9.42e-19 78.2
Msa0034770 MtrunA17_Chr7g0246761 38.017 121 60 5 32 147 22 132 1.08e-18 78.6
Msa0034770 MtrunA17_Chr3g0128771 32.886 149 85 6 5 149 26 163 1.78e-18 77.4
Msa0034770 MtrunA17_Chr6g0486651 36.000 125 68 4 25 147 47 161 2.70e-18 80.1
Msa0034770 MtrunA17_Chr7g0248831 40.541 74 44 0 80 153 83 156 7.53e-18 75.9
Msa0034770 MtrunA17_Chr1g0198201 31.746 126 84 2 25 149 8 132 1.15e-17 76.6
Msa0034770 MtrunA17_Chr2g0325141 46.154 65 31 1 87 147 69 133 2.86e-17 73.6
Msa0034770 MtrunA17_Chr4g0030831 26.667 150 87 3 18 147 8 154 3.20e-17 74.3
Msa0034770 MtrunA17_Chr3g0132491 35.156 128 67 6 25 147 62 178 4.98e-17 76.6
Msa0034770 MtrunA17_Chr5g0407431 30.714 140 83 4 10 148 22 148 7.20e-17 74.3
Msa0034770 MtrunA17_Chr6g0470751 47.619 63 32 1 86 147 106 168 1.06e-16 75.1
Msa0034770 MtrunA17_Chr8g0348151 29.252 147 86 3 21 153 20 162 1.65e-16 73.2
Msa0034770 MtrunA17_Chr8g0364881 31.655 139 84 5 20 149 2 138 1.96e-16 74.7
Msa0034770 MtrunA17_Chr2g0325161 43.662 71 36 1 81 147 95 165 2.12e-16 73.6
Msa0034770 MtrunA17_Chr7g0273051 30.667 150 86 7 2 147 27 162 2.28e-16 74.7
Msa0034770 MtrunA17_Chr7g0244851 32.026 153 91 5 2 153 15 155 3.19e-16 71.6
Msa0034770 MtrunA17_Chr5g0409211 29.452 146 91 5 12 153 5 142 3.35e-16 71.6
Msa0034770 MtrunA17_Chr1g0191601 32.800 125 75 4 25 147 52 169 3.86e-16 73.9
Msa0034770 MtrunA17_Chr1g0203541 42.647 68 36 1 83 147 111 178 3.89e-16 72.0
Msa0034770 MtrunA17_Chr8g0383921 29.221 154 99 3 1 153 42 186 5.87e-16 71.6
Msa0034770 MtrunA17_Chr8g0350741 34.127 126 72 2 27 147 12 131 6.84e-16 70.9
Msa0034770 MtrunA17_Chr5g0396601 30.952 126 82 2 24 147 270 392 8.74e-16 73.2
Msa0034770 MtrunA17_Chr8g0370171 28.472 144 89 4 5 147 10 140 9.03e-16 71.2
Msa0034770 MtrunA17_Chr5g0426871 41.791 67 36 2 83 147 114 179 1.46e-15 72.4
Msa0034770 MtrunA17_Chr2g0327791 37.647 85 44 2 66 147 99 177 1.58e-15 70.9
Msa0034770 MtrunA17_Chr2g0301871 44.928 69 35 2 87 153 64 131 1.82e-15 71.6
Msa0034770 MtrunA17_Chr8g0388231 31.452 124 78 2 26 149 265 381 1.97e-15 72.0
Msa0034770 MtrunA17_Chr3g0133001 52.000 50 24 0 98 147 149 198 3.38e-15 71.6
Msa0034770 MtrunA17_Chr5g0396611 28.667 150 96 4 3 147 239 382 4.62e-15 71.2
Msa0034770 MtrunA17_Chr6g0451031 34.921 126 63 5 22 147 5 111 4.75e-15 67.4
Msa0034770 MtrunA17_Chr7g0248841 52.000 50 24 0 105 154 104 153 5.14e-15 68.2
Msa0034770 MtrunA17_Chr3g0129671 32.237 152 81 6 5 148 42 179 5.37e-15 69.3
Msa0034770 MtrunA17_Chr5g0413841 28.986 138 83 5 25 149 51 186 8.43e-15 70.1
Msa0034770 MtrunA17_Chr6g0451051 33.333 123 61 3 25 147 7 108 1.09e-14 67.0
Msa0034770 MtrunA17_Chr5g0396591 29.134 127 85 2 23 147 159 282 1.50e-14 68.9
Msa0034770 MtrunA17_Chr8g0369571 32.374 139 81 5 17 153 11 138 1.57e-14 67.4
Msa0034770 MtrunA17_Chr3g0123631 29.839 124 73 4 26 147 6 117 1.61e-14 67.4
Msa0034770 MtrunA17_Chr7g0275601 30.882 136 82 4 22 153 29 156 1.96e-14 66.6
Msa0034770 MtrunA17_Chr8g0366561 28.777 139 89 4 22 153 55 190 2.20e-14 68.9
Msa0034770 MtrunA17_Chr5g0427431 34.524 84 46 2 64 147 128 202 2.58e-14 68.9
Msa0034770 MtrunA17_Chr5g0433861 28.676 136 77 3 21 147 24 148 2.63e-14 68.6
Msa0034770 MtrunA17_Chr4g0013561 42.254 71 38 1 84 154 92 159 2.90e-14 67.4
Msa0034770 MtrunA17_Chr3g0123641 42.857 63 33 2 87 147 56 117 3.54e-14 67.4
Msa0034770 MtrunA17_Chr8g0366521 40.000 70 40 1 80 147 79 148 4.72e-14 67.8
Msa0034770 MtrunA17_Chr5g0426781 28.571 168 92 6 2 147 35 196 5.41e-14 67.8
Msa0034770 MtrunA17_Chr3g0113811 27.465 142 90 4 13 149 17 150 5.58e-14 65.9
Msa0034770 MtrunA17_Chr8g0381731 39.655 58 35 0 90 147 159 216 5.99e-14 67.4
Msa0034770 MtrunA17_Chr5g0406031 38.710 62 38 0 87 148 171 232 6.09e-14 66.6
Msa0034770 MtrunA17_Chr5g0409201 48.000 50 26 0 104 153 92 141 1.01e-13 64.7
Msa0034770 MtrunA17_Chr6g0451161 40.580 69 38 2 80 147 135 201 1.07e-13 67.0
Msa0034770 MtrunA17_Chr8g0336881 40.278 72 42 1 83 153 82 153 1.39e-13 66.6
Msa0034770 MtrunA17_Chr4g0073141 48.980 49 25 0 103 151 142 190 2.24e-13 66.2
Msa0034770 MtrunA17_Chr8g0388201 39.394 66 40 0 82 147 324 389 2.84e-13 65.9
Msa0034770 MtrunA17_Chr5g0444161 48.837 43 22 0 105 147 185 227 2.96e-13 64.7
Msa0034770 MtrunA17_Chr7g0261091 25.191 131 86 3 25 149 20 144 4.05e-13 65.5
Msa0034770 MtrunA17_Chr3g0105441 37.209 86 51 2 67 149 91 176 4.34e-13 64.7
Msa0034770 MtrunA17_Chr6g0451041 49.057 53 27 0 95 147 8 60 4.43e-13 61.6
Msa0034770 MtrunA17_Chr8g0383901 41.176 68 39 1 86 153 113 179 4.51e-13 63.9
Msa0034770 MtrunA17_Chr2g0297011 51.111 45 22 0 104 148 101 145 4.54e-13 63.2
Msa0034770 MtrunA17_Chr2g0307411 47.917 48 25 0 100 147 95 142 4.60e-13 63.5
Msa0034770 MtrunA17_Chr8g0383931 41.176 68 39 1 86 153 123 189 4.71e-13 64.3
Msa0034770 MtrunA17_Chr8g0371291 44.000 50 28 0 99 148 174 223 4.90e-13 64.3
Msa0034770 MtrunA17_Chr1g0167811 36.508 63 38 1 89 149 105 167 6.16e-13 64.7
Msa0034770 MtrunA17_Chr5g0411081 39.655 58 35 0 95 152 138 195 6.52e-13 65.1
Msa0034770 MtrunA17_Chr8g0383871 38.889 72 43 1 82 153 117 187 6.73e-13 63.9
Msa0034770 MtrunA17_Chr5g0444141 48.837 43 22 0 105 147 220 262 8.05e-13 63.9
Msa0034770 MtrunA17_Chr5g0437361 35.294 68 44 0 82 149 80 147 1.02e-12 62.4
Msa0034770 MtrunA17_Chr3g0117821 36.923 65 41 0 83 147 63 127 1.09e-12 62.0
Msa0034770 MtrunA17_Chr2g0279431 47.826 46 24 0 102 147 158 203 1.38e-12 63.9
Msa0034770 MtrunA17_Chr7g0276591 43.137 51 29 0 103 153 99 149 1.42e-12 63.5
Msa0034770 MtrunA17_Chr1g0186661 38.462 52 32 0 98 149 124 175 1.46e-12 63.9
Msa0034770 MtrunA17_Chr3g0118061 34.921 63 41 0 85 147 84 146 1.51e-12 62.8
Msa0034770 MtrunA17_Chr8g0347101 32.432 74 50 0 80 153 289 362 1.75e-12 63.5
Msa0034770 MtrunA17_Chr8g0383941 26.797 153 97 4 2 153 44 182 1.95e-12 62.4
Msa0034770 MtrunA17_Chr4g0067591 39.216 51 31 0 97 147 96 146 2.06e-12 63.2
Msa0034770 MtrunA17_Chr3g0144241 52.273 44 21 0 105 148 104 147 2.61e-12 61.6
Msa0034770 MtrunA17_Chr7g0274721 39.344 61 34 1 87 147 78 135 2.86e-12 61.2
Msa0034770 MtrunA17_Chr8g0383881 26.316 152 93 3 12 153 36 178 3.24e-12 61.6
Msa0034770 MtrunA17_Chr3g0126611 35.294 68 44 0 82 149 308 375 4.11e-12 62.8
Msa0034770 MtrunA17_Chr4g0050041 51.163 43 21 0 105 147 152 194 4.44e-12 62.4
Msa0034770 MtrunA17_Chr1g0184171 44.444 45 25 0 105 149 130 174 4.79e-12 62.4
Msa0034770 MtrunA17_Chr4g0052671 51.163 43 21 0 105 147 106 148 6.23e-12 59.7
Msa0034770 MtrunA17_Chr8g0383861 29.710 138 90 5 17 153 57 188 7.00e-12 60.8
Msa0034770 MtrunA17_Chr4g0059281 42.105 57 33 0 98 154 125 181 1.12e-11 61.2
Msa0034770 MtrunA17_Chr8g0345361 47.826 46 24 0 102 147 114 159 1.46e-11 60.5
Msa0034770 MtrunA17_Chr7g0274731 38.983 59 33 1 89 147 101 156 1.50e-11 59.7
Msa0034770 MtrunA17_Chr3g0118071 36.066 61 39 0 87 147 86 146 2.26e-11 59.7
Msa0034770 MtrunA17_Chr2g0300331 31.298 131 74 5 24 147 90 211 3.07e-11 59.3
Msa0034770 MtrunA17_Chr5g0430781 32.558 86 53 1 62 147 72 152 3.12e-11 59.3
Msa0034770 MtrunA17_Chr2g0295571 37.931 58 36 0 90 147 104 161 4.16e-11 59.3
Msa0034770 MtrunA17_Chr7g0269491 30.120 83 56 1 67 147 54 136 4.93e-11 58.2
Msa0034770 MtrunA17_Chr4g0060981 37.143 70 44 0 79 148 148 217 5.05e-11 58.5
Msa0034770 MtrunA17_Chr8g0369691 41.538 65 35 3 84 147 425 487 7.66e-11 59.3
Msa0034770 MtrunA17_Chr7g0263911 50.000 48 24 0 104 151 589 636 8.75e-11 58.9
Msa0034770 MtrunA17_Chr7g0274711 32.812 64 42 1 85 147 75 138 9.51e-11 57.4
Msa0034770 MtrunA17_Chr1g0181401 40.000 65 36 1 88 152 469 530 9.86e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0034770 AT5G06490.1 34.463 177 87 6 5 153 2 177 6.79e-25 95.1
Msa0034770 AT2G35910.1 51.351 74 34 1 80 153 124 195 1.12e-22 89.7
Msa0034770 AT4G10160.1 33.094 139 85 3 11 149 11 141 2.68e-19 81.3
Msa0034770 AT5G53110.1 34.146 123 75 2 27 149 258 374 2.34e-18 80.9
Msa0034770 AT2G46493.1 27.200 125 89 1 25 149 60 182 3.23e-18 77.4
Msa0034770 AT1G23980.1 31.343 134 79 4 25 147 55 186 1.63e-17 78.2
Msa0034770 AT2G46160.1 43.243 74 36 1 86 153 113 186 5.97e-17 74.7
Msa0034770 AT2G46160.2 43.243 74 36 1 86 153 113 186 5.97e-17 74.7
Msa0034770 AT1G76410.1 29.730 148 89 4 2 148 14 147 7.81e-17 73.9
Msa0034770 AT4G28890.1 32.215 149 85 5 10 147 22 165 1.09e-16 76.3
Msa0034770 AT1G28040.1 29.457 129 83 2 26 149 172 297 1.71e-16 74.7
Msa0034770 AT2G35000.1 30.709 127 79 2 22 147 59 177 4.13e-16 74.3
Msa0034770 AT4G10150.1 33.835 133 72 6 24 149 32 155 4.96e-16 72.8
Msa0034770 AT1G20823.1 31.333 150 92 6 4 148 11 154 5.22e-16 72.0
Msa0034770 AT2G25410.1 30.070 143 87 4 14 147 231 369 9.69e-16 73.2
Msa0034770 AT3G61550.1 47.368 57 30 0 97 153 128 184 9.73e-16 71.6
Msa0034770 AT4G40070.1 31.081 148 90 4 10 154 35 173 1.12e-15 72.8
Msa0034770 AT2G34990.1 26.923 130 93 2 19 147 10 138 1.27e-15 72.4
Msa0034770 AT5G27420.1 38.372 86 47 3 64 147 85 166 1.56e-15 72.8
Msa0034770 AT2G34990.2 26.923 130 93 2 19 147 32 160 1.57e-15 72.4
Msa0034770 AT3G05200.1 45.455 66 33 2 84 147 106 170 2.10e-15 72.4
Msa0034770 AT1G35330.1 43.939 66 34 2 86 149 109 173 2.31e-15 72.0
Msa0034770 AT2G20030.1 31.343 134 71 6 23 147 45 166 3.73e-15 71.6
Msa0034770 AT5G05280.1 54.000 50 23 0 102 151 110 159 4.66e-15 68.9
Msa0034770 AT5G07040.1 29.197 137 87 4 21 153 12 142 8.34e-15 67.8
Msa0034770 AT1G72200.1 40.000 65 38 1 84 147 122 186 1.60e-14 70.1
Msa0034770 AT1G04360.1 30.597 134 78 3 25 149 51 178 1.77e-14 69.7
Msa0034770 AT4G17905.2 46.032 63 31 1 88 147 161 223 1.88e-14 69.7
Msa0034770 AT4G17905.1 46.032 63 31 1 88 147 135 197 2.03e-14 69.3
Msa0034770 AT4G09120.1 34.940 83 51 2 67 147 84 165 2.04e-14 69.3
Msa0034770 AT3G48030.2 32.283 127 79 4 26 149 51 173 2.30e-14 68.6
Msa0034770 AT3G03550.1 43.077 65 37 0 90 154 144 208 2.70e-14 69.3
Msa0034770 AT1G72310.1 39.773 88 43 3 67 152 95 174 3.01e-14 68.9
Msa0034770 AT3G48030.1 39.130 69 41 1 81 149 184 251 3.49e-14 68.9
Msa0034770 AT5G05810.1 49.020 51 26 0 97 147 138 188 4.01e-14 68.9
Msa0034770 AT2G46494.1 29.688 128 75 3 33 149 237 360 4.92e-14 68.6
Msa0034770 AT3G62690.1 43.284 67 36 1 81 147 91 155 6.70e-14 67.4
Msa0034770 AT2G47560.1 44.262 61 34 0 87 147 90 150 6.87e-14 67.0
Msa0034770 AT3G10910.1 50.000 50 25 0 102 151 111 160 7.21e-14 65.9
Msa0034770 AT4G09130.1 39.706 68 34 2 84 147 98 162 7.23e-14 68.2
Msa0034770 AT4G33565.1 45.283 53 29 0 95 147 207 259 7.99e-14 67.8
Msa0034770 AT4G09110.1 32.394 142 83 6 8 147 35 165 8.61e-14 67.4
Msa0034770 AT2G18650.1 48.077 52 27 0 101 152 122 173 8.93e-14 67.8
Msa0034770 AT5G17600.1 43.103 58 33 0 90 147 127 184 1.53e-13 67.0
Msa0034770 AT3G18773.1 31.333 150 90 6 1 147 33 172 1.72e-13 65.5
Msa0034770 AT5G01880.1 46.939 49 26 0 101 149 101 149 1.78e-13 64.3
Msa0034770 AT1G22500.1 32.283 127 79 5 23 147 39 160 1.99e-13 66.6
Msa0034770 AT5G66070.5 35.802 81 45 2 68 148 146 219 2.32e-13 65.5
Msa0034770 AT5G66070.1 35.802 81 45 2 68 148 146 219 2.32e-13 65.5
Msa0034770 AT5G66070.2 35.802 81 45 2 68 148 170 243 2.53e-13 65.5
Msa0034770 AT2G46495.1 28.378 148 87 6 11 149 227 364 2.54e-13 66.6
Msa0034770 AT5G57750.1 30.000 130 77 3 27 147 40 164 2.58e-13 65.1
Msa0034770 AT4G35480.1 30.000 150 96 4 6 148 9 156 2.60e-13 64.7
Msa0034770 AT4G30400.1 51.111 45 22 0 103 147 132 176 2.64e-13 66.6
Msa0034770 AT2G42360.1 51.020 49 24 0 99 147 102 150 3.07e-13 65.1
Msa0034770 AT3G16720.1 51.111 45 22 0 103 147 117 161 3.66e-13 65.9
Msa0034770 AT3G20395.1 53.191 47 22 0 101 147 167 213 3.79e-13 64.7
Msa0034770 AT2G42350.1 48.980 49 25 0 105 153 100 148 3.84e-13 64.7
Msa0034770 AT2G17450.1 30.070 143 85 4 10 148 14 145 4.15e-13 63.9
Msa0034770 AT5G10380.1 46.000 50 27 0 102 151 132 181 4.18e-13 65.5
Msa0034770 AT2G27940.1 40.299 67 37 1 87 153 125 188 4.22e-13 64.7
Msa0034770 AT4G09100.1 35.211 71 45 1 82 151 61 131 4.82e-13 62.8
Msa0034770 AT4G35840.1 29.457 129 81 3 30 148 106 234 5.12e-13 64.7
Msa0034770 AT1G33480.1 53.488 43 20 0 105 147 100 142 5.63e-13 64.7
Msa0034770 AT5G36001.1 38.462 65 36 1 89 149 244 308 8.63e-13 64.7
Msa0034770 AT1G74410.1 51.111 45 22 0 105 149 176 220 9.34e-13 63.5
Msa0034770 AT1G71980.1 43.056 72 38 3 84 154 212 281 1.22e-12 64.7
Msa0034770 AT1G72220.1 48.837 43 22 0 105 147 177 219 1.26e-12 64.7
Msa0034770 AT1G49210.1 32.117 137 83 5 12 147 50 177 1.33e-12 63.2
Msa0034770 AT2G17730.1 35.526 76 48 1 74 148 164 239 1.50e-12 63.5
Msa0034770 AT4G15975.1 45.652 46 25 0 102 147 73 118 1.70e-12 63.2
Msa0034770 AT1G49220.1 32.117 137 83 5 12 147 50 177 1.83e-12 63.2
Msa0034770 AT2G44578.1 53.488 43 20 0 105 147 70 112 2.25e-12 61.2
Msa0034770 AT1G35630.1 32.800 125 67 6 29 148 164 276 2.41e-12 63.5
Msa0034770 AT3G14320.1 48.936 47 24 0 102 148 85 131 2.42e-12 62.4
Msa0034770 AT1G53820.1 45.833 48 26 0 102 149 117 164 2.49e-12 63.5
Msa0034770 AT5G40250.1 51.111 45 22 0 103 147 141 185 3.12e-12 63.5
Msa0034770 AT1G49200.1 31.655 139 81 6 12 147 51 178 3.85e-12 62.0
Msa0034770 AT3G18930.2 33.735 83 47 1 80 154 123 205 4.01e-12 63.2
Msa0034770 AT3G18930.3 33.735 83 47 1 80 154 123 205 4.01e-12 63.2
Msa0034770 AT3G18930.1 33.735 83 47 1 80 154 123 205 4.01e-12 63.2
Msa0034770 AT1G49230.1 31.159 138 78 6 14 147 49 173 4.05e-12 62.0
Msa0034770 AT5G58580.1 53.061 49 23 0 105 153 138 186 5.64e-12 62.4
Msa0034770 AT3G60220.1 37.500 72 44 1 83 153 94 165 6.37e-12 62.4
Msa0034770 AT1G35625.1 36.364 77 46 3 73 148 202 276 7.92e-12 62.0
Msa0034770 AT5G43420.1 43.182 44 25 0 104 147 137 180 8.12e-12 62.4
Msa0034770 AT2G46495.2 37.313 67 41 1 84 149 270 336 9.69e-12 62.0
Msa0034770 AT1G32361.1 37.313 67 38 2 83 149 136 198 1.00e-11 62.0
Msa0034770 AT2G35420.1 40.323 62 35 1 86 147 86 145 1.02e-11 61.2
Msa0034770 AT1G80400.1 31.469 143 72 6 30 154 273 407 1.18e-11 61.6
Msa0034770 AT5G37270.1 40.278 72 38 2 82 148 127 198 1.91e-11 60.1
Msa0034770 AT5G37250.1 40.278 72 38 2 82 148 111 182 1.98e-11 59.7
Msa0034770 AT5G37250.2 40.278 72 38 2 82 148 127 198 2.04e-11 59.7
Msa0034770 AT4G38140.1 45.283 53 28 1 103 154 60 112 2.37e-11 58.5
Msa0034770 AT1G22670.1 50.000 56 26 2 96 150 223 277 2.48e-11 60.8
Msa0034770 AT3G55530.1 31.522 92 60 2 58 147 162 252 2.79e-11 60.1
Msa0034770 AT1G53010.1 37.037 81 45 2 72 148 95 173 4.02e-11 58.5
Msa0034770 AT5G37230.1 38.889 72 39 2 82 148 127 198 4.16e-11 58.9
Msa0034770 AT3G11110.1 51.163 43 21 0 107 149 109 151 8.99e-11 57.4
Msa0034770 AT4G26400.2 45.833 48 25 1 100 147 236 282 9.45e-11 58.9
Msa0034770 AT4G26400.1 45.833 48 25 1 100 147 236 282 9.45e-11 58.9

Find 23 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATCTTAATACTTGTATT+TGG 0.241998 1_1:+62766584 Msa0034770:CDS
TGTATTTGGTCTCGTATTCT+TGG 0.251746 1_1:+62766598 Msa0034770:CDS
TCTGAGAGTATAAAAGTTTC+GGG 0.311980 1_1:-62766772 None:intergenic
ATCTGAGAGTATAAAAGTTT+CGG 0.329103 1_1:-62766773 None:intergenic
ATTAGTTGCTCTATTTGCTT+AGG 0.404219 1_1:+62766821 Msa0034770:CDS
TACTCTCAGATTCAGAAGAA+TGG 0.427690 1_1:+62766785 Msa0034770:CDS
ATGACGATGTGGTGGAAAAC+CGG 0.430831 1_1:-62766690 None:intergenic
TGATAAAGATGACCACAATC+TGG 0.456707 1_1:-62766878 None:intergenic
ATGTTGCTTGTGTTGATCCA+TGG 0.521110 1_1:+62766900 Msa0034770:CDS
TTGAGGTTACTTCCAGATTG+TGG 0.535101 1_1:+62766866 Msa0034770:CDS
TCGTCATGAAGACTCAATCA+TGG 0.545902 1_1:+62766706 Msa0034770:CDS
ATAAAGAAAGTGACATGTTG+AGG 0.576166 1_1:+62766849 Msa0034770:CDS
TCAAAACCGTTGTTGTTGTG+TGG 0.584708 1_1:-62766545 None:intergenic
GTGGAATGTAACATGAGCCA+TGG 0.599564 1_1:-62766917 None:intergenic
AAACCGTTGTTGTTGTGTGG+AGG 0.603017 1_1:-62766542 None:intergenic
GTTGTTGTGTGGAGGTTCCG+AGG 0.639516 1_1:-62766534 None:intergenic
GTATCCTTCAAAACTTGTGT+CGG 0.641573 1_1:-62766750 None:intergenic
GAGTCTTCATGACGATGTGG+TGG 0.660756 1_1:-62766698 None:intergenic
GAGCAACAACAACGGCACCT+CGG 0.664145 1_1:+62766517 Msa0034770:CDS
GAAGACTCAATCATGGAACA+AGG 0.664922 1_1:+62766713 Msa0034770:CDS
GAACCTCCACACAACAACAA+CGG 0.709301 1_1:+62766539 Msa0034770:CDS
ATTGAGTCTTCATGACGATG+TGG 0.716521 1_1:-62766701 None:intergenic
AAGAAGATGAGCAACAACAA+CGG 0.717539 1_1:+62766509 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATCTTAATACTTGTATT+TGG + chr1_1:62766584-62766603 Msa0034770:CDS 15.0%
!! AACATTCTCAATATTTTGTC+CGG + chr1_1:62766671-62766690 Msa0034770:CDS 25.0%
!! ATCTGAGAGTATAAAAGTTT+CGG - chr1_1:62766776-62766795 None:intergenic 25.0%
!! AGTGATGATTTTCTACATAT+GGG - chr1_1:62766947-62766966 None:intergenic 25.0%
!! TAGTGATGATTTTCTACATA+TGG - chr1_1:62766948-62766967 None:intergenic 25.0%
! TCTGAGAGTATAAAAGTTTC+GGG - chr1_1:62766775-62766794 None:intergenic 30.0%
ATTAGTTGCTCTATTTGCTT+AGG + chr1_1:62766821-62766840 Msa0034770:CDS 30.0%
ATAAAGAAAGTGACATGTTG+AGG + chr1_1:62766849-62766868 Msa0034770:CDS 30.0%
TGTATTTGGTCTCGTATTCT+TGG + chr1_1:62766598-62766617 Msa0034770:CDS 35.0%
GTATCCTTCAAAACTTGTGT+CGG - chr1_1:62766753-62766772 None:intergenic 35.0%
TACTCTCAGATTCAGAAGAA+TGG + chr1_1:62766785-62766804 Msa0034770:CDS 35.0%
TGATAAAGATGACCACAATC+TGG - chr1_1:62766881-62766900 None:intergenic 35.0%
! TTTTCTACATATGGGACAAG+TGG - chr1_1:62766939-62766958 None:intergenic 35.0%
! TCAAAACCGTTGTTGTTGTG+TGG - chr1_1:62766548-62766567 None:intergenic 40.0%
!! AACAACGGTTTTGACGCAAA+TGG + chr1_1:62766554-62766573 Msa0034770:CDS 40.0%
!! ACAACGGTTTTGACGCAAAT+GGG + chr1_1:62766555-62766574 Msa0034770:CDS 40.0%
ATTGAGTCTTCATGACGATG+TGG - chr1_1:62766704-62766723 None:intergenic 40.0%
TCGTCATGAAGACTCAATCA+TGG + chr1_1:62766706-62766725 Msa0034770:CDS 40.0%
GAAGACTCAATCATGGAACA+AGG + chr1_1:62766713-62766732 Msa0034770:CDS 40.0%
!! CATACCGACACAAGTTTTGA+AGG + chr1_1:62766746-62766765 Msa0034770:CDS 40.0%
TTGAGGTTACTTCCAGATTG+TGG + chr1_1:62766866-62766885 Msa0034770:CDS 40.0%
! ATGTTGCTTGTGTTGATCCA+TGG + chr1_1:62766900-62766919 Msa0034770:CDS 40.0%
GAACCTCCACACAACAACAA+CGG + chr1_1:62766539-62766558 Msa0034770:CDS 45.0%
! AAACCGTTGTTGTTGTGTGG+AGG - chr1_1:62766545-62766564 None:intergenic 45.0%
! ATGACGATGTGGTGGAAAAC+CGG - chr1_1:62766693-62766712 None:intergenic 45.0%
GTGGAATGTAACATGAGCCA+TGG - chr1_1:62766920-62766939 None:intergenic 45.0%
GAGTCTTCATGACGATGTGG+TGG - chr1_1:62766701-62766720 None:intergenic 50.0%
GAGCAACAACAACGGCACCT+CGG + chr1_1:62766517-62766536 Msa0034770:CDS 55.0%
GTTGTTGTGTGGAGGTTCCG+AGG - chr1_1:62766537-62766556 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 62766515 62766979 62766515 ID=Msa0034770;Name=Msa0034770
chr1_1 mRNA 62766515 62766979 62766515 ID=Msa0034770-mRNA-1;Parent=Msa0034770;Name=Msa0034770-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|154
chr1_1 exon 62766515 62766979 62766515 ID=Msa0034770-mRNA-1:exon:19882;Parent=Msa0034770-mRNA-1
chr1_1 CDS 62766515 62766979 62766515 ID=Msa0034770-mRNA-1:cds;Parent=Msa0034770-mRNA-1
Gene Sequence

>Msa0034770

ATGAGCAACAACAACGGCACCTCGGAACCTCCACACAACAACAACGGTTTTGACGCAAATGGGTTCACATATATCTTAATACTTGTATTTGGTCTCGTATTCTTGGTTCTATCAATAATTGTTGCTTGTATTCGACTTCGTAGTTCACAAAGTCGAAACATTCTCAATATTTTGTCCGGTTTTCCACCACATCGTCATGAAGACTCAATCATGGAACAAGGACTTCGTCATCATACCGACACAAGTTTTGAAGGATACCCGAAACTTTTATACTCTCAGATTCAGAAGAATGGTTCTTCAACTGCAATTAGTTGCTCTATTTGCTTAGGAGACTATAAAGAAAGTGACATGTTGAGGTTACTTCCAGATTGTGGTCATCTTTATCATGTTGCTTGTGTTGATCCATGGCTCATGTTACATTCCACTTGTCCCATATGTAGAAAATCATCACTAGCTTCATCCTAG
Protein sequence

>Msa0034770

MSNNNGTSEPPHNNNGFDANGFTYILILVFGLVFLVLSIIVACIRLRSSQSRNILNILSGFPPHRHEDSIMEQGLRHHTDTSFEGYPKLLYSQIQKNGSSTAISCSICLGDYKESDMLRLLPDCGHLYHVACVDPWLMLHSTCPICRKSSLASS*