AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645150 MtrunA17_Chr4g0032581 97.386 153 4 0 1 153 1 153 1.23e-107 303
Msa0645150 MtrunA17_Chr4g0032291 80.833 120 22 1 35 153 46 165 6.00e-63 190
Msa0645150 MtrunA17_Chr4g0032571 73.333 135 34 2 9 142 4 137 3.24e-56 172
Msa0645150 MtrunA17_Chr7g0240831 45.455 143 70 4 8 147 2 139 4.50e-35 119
Msa0645150 MtrunA17_Chr7g0240841 45.455 143 70 4 8 147 2 139 4.50e-35 119
Msa0645150 MtrunA17_Chr4g0008841 45.217 115 62 1 32 145 13 127 5.88e-32 110
Msa0645150 MtrunA17_Chr7g0240861 50.427 117 55 2 17 130 12 128 2.10e-30 107
Msa0645150 MtrunA17_Chr1g0150721 38.095 126 57 5 31 135 1 126 5.40e-17 72.8
Msa0645150 MtrunA17_Chr3g0136431 34.375 128 60 3 31 134 1 128 5.63e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645150 AT2G26975.1 52.381 147 57 5 5 145 1 140 2.74e-40 132
Msa0645150 AT5G59030.1 44.643 168 78 5 1 153 1 168 1.88e-33 116
Msa0645150 AT3G46900.1 48.760 121 58 3 36 152 34 154 2.91e-32 112
Msa0645150 AT5G59040.1 44.262 122 67 1 30 150 28 149 5.41e-29 104
Msa0645150 AT2G37925.1 42.982 114 61 2 30 139 28 141 1.54e-25 95.1
Msa0645150 AT5G20650.1 37.692 130 55 4 31 134 1 130 1.20e-14 67.0

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTTGGCCTAGCTTATT+TGG 0.201432 4_3:-49621978 Msa0645150:CDS
TTTCAATGGTGGAGTGTTCT+TGG 0.286706 4_3:-49621936 Msa0645150:CDS
CATTGATTCTCTTTGACAAT+TGG 0.313757 4_3:-49622150 Msa0645150:CDS
CTGTGCTTGGTCATGCTGTT+GGG 0.361371 4_3:-49621910 Msa0645150:CDS
CCTAGCTTATTTGGTTATGT+TGG 0.373927 4_3:-49621969 Msa0645150:CDS
CTCTTTGACAATTGGCCTGC+TGG 0.377320 4_3:-49622142 Msa0645150:CDS
TGAACTAGACCGGCCACAAC+AGG 0.384941 4_3:+49622019 None:intergenic
GGCCACAACAGGGTTTGAAC+CGG 0.386419 4_3:+49622030 None:intergenic
GCTGTGCTTGGTCATGCTGT+TGG 0.390679 4_3:-49621911 Msa0645150:CDS
AATTGGCCTGCTGGTAGTTC+TGG 0.393131 4_3:-49622133 Msa0645150:CDS
TGAGAGAAACTCGATGAGTA+TGG 0.396012 4_3:+49622072 None:intergenic
GTGTTCTTGGTTGCTGTGCT+TGG 0.406869 4_3:-49621923 Msa0645150:CDS
TTGAACCGGGTTTAATGAAC+CGG 0.410106 4_3:+49622044 None:intergenic
ACCCATCATCATAGTGTCAT+TGG 0.428445 4_3:+49622219 None:intergenic
TTGGCTCTCATGTCTTTCAA+TGG 0.439850 4_3:-49621950 Msa0645150:CDS
TACCAATGACACTATGATGA+TGG 0.457064 4_3:-49622221 Msa0645150:CDS
GCCACAACAGGGTTTGAACC+GGG 0.512994 4_3:+49622031 None:intergenic
ATGGATCATGGCATGGATCA+TGG 0.518842 4_3:-49622274 Msa0645150:CDS
GCTCTCATGTCTTTCAATGG+TGG 0.527046 4_3:-49621947 Msa0645150:CDS
AACTGATACAGCATGGATCA+TGG 0.527414 4_3:-49622286 None:intergenic
TAGTTCTGGTAAGTATGTAT+TGG 0.530240 4_3:-49622119 Msa0645150:CDS
GGTTCAAACCCTGTTGTGGC+CGG 0.535871 4_3:-49622028 Msa0645150:CDS
TTGGTAGATGATGATGACTT+TGG 0.542496 4_3:+49622238 None:intergenic
CCAACATAACCAAATAAGCT+AGG 0.547948 4_3:+49621969 None:intergenic
TCGAAATTCTCATCTTGATG+TGG 0.556567 4_3:+49621857 None:intergenic
ACCCGGTTCAAACCCTGTTG+TGG 0.557595 4_3:-49622032 Msa0645150:CDS
TCGAGTTTCTCTCAAGCACC+CGG 0.561462 4_3:-49622063 Msa0645150:CDS
CAAAAGTGTCTGAACTAGAC+CGG 0.563621 4_3:+49622009 None:intergenic
ACCAATGACACTATGATGAT+GGG 0.572192 4_3:-49622220 Msa0645150:CDS
CATGGCGAAAACAAGGATCA+AGG 0.574469 4_3:+49622096 None:intergenic
AGCACCCGGTTCATTAAACC+CGG 0.579765 4_3:-49622049 Msa0645150:CDS
TGAACCGGGTTTAATGAACC+GGG 0.596910 4_3:+49622045 None:intergenic
GTATGGACATGGCGAAAACA+AGG 0.607210 4_3:+49622089 None:intergenic
TCATGGCATGGATCATGGAA+TGG 0.632980 4_3:-49622269 Msa0645150:CDS
TACTTACCAGAACTACCAGC+AGG 0.638786 4_3:+49622127 None:intergenic
CATGGATCATGGAATGGCAA+TGG 0.647968 4_3:-49622263 Msa0645150:CDS
ATACAGCATGGATCATGGCA+TGG 0.657843 4_3:-49622281 None:intergenic
AAACTCGATGAGTATGGACA+TGG 0.667480 4_3:+49622078 None:intergenic
GAACTAGACCGGCCACAACA+GGG 0.667595 4_3:+49622020 None:intergenic
AATAATTAACAAGATAACGG+AGG 0.670863 4_3:+49621830 None:intergenic
ATGCACATGACATTCTTCTG+CGG 0.705097 4_3:-49622181 Msa0645150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTGATTCTCTTTGACAAT+TGG - chr4_3:49621959-49621978 Msa0645150:CDS 30.0%
!! TAGTTCTGGTAAGTATGTAT+TGG - chr4_3:49621990-49622009 Msa0645150:CDS 30.0%
! TTGGTAGATGATGATGACTT+TGG + chr4_3:49621874-49621893 None:intergenic 35.0%
TACCAATGACACTATGATGA+TGG - chr4_3:49621888-49621907 Msa0645150:CDS 35.0%
ACCAATGACACTATGATGAT+GGG - chr4_3:49621889-49621908 Msa0645150:CDS 35.0%
! AGACACTTTTGCATGTTCTT+AGG - chr4_3:49622112-49622131 Msa0645150:CDS 35.0%
! GACACTTTTGCATGTTCTTA+GGG - chr4_3:49622113-49622132 Msa0645150:CDS 35.0%
CCAACATAACCAAATAAGCT+AGG + chr4_3:49622143-49622162 None:intergenic 35.0%
CCTAGCTTATTTGGTTATGT+TGG - chr4_3:49622140-49622159 Msa0645150:CDS 35.0%
!!! GATGTGGTTTTTTGAAAGCA+CGG + chr4_3:49622239-49622258 None:intergenic 35.0%
TCGAAATTCTCATCTTGATG+TGG + chr4_3:49622255-49622274 None:intergenic 35.0%
ACCCATCATCATAGTGTCAT+TGG + chr4_3:49621893-49621912 None:intergenic 40.0%
ATGCACATGACATTCTTCTG+CGG - chr4_3:49621928-49621947 Msa0645150:CDS 40.0%
AAACTCGATGAGTATGGACA+TGG + chr4_3:49622034-49622053 None:intergenic 40.0%
TGAGAGAAACTCGATGAGTA+TGG + chr4_3:49622040-49622059 None:intergenic 40.0%
TTGAACCGGGTTTAATGAAC+CGG + chr4_3:49622068-49622087 None:intergenic 40.0%
!! CAAAAGTGTCTGAACTAGAC+CGG + chr4_3:49622103-49622122 None:intergenic 40.0%
! CTTTTGCATGTTCTTAGGGT+TGG - chr4_3:49622117-49622136 Msa0645150:CDS 40.0%
! TTGGCTCTCATGTCTTTCAA+TGG - chr4_3:49622159-49622178 Msa0645150:CDS 40.0%
! TTTCAATGGTGGAGTGTTCT+TGG - chr4_3:49622173-49622192 Msa0645150:CDS 40.0%
ATGGATCATGGCATGGATCA+TGG - chr4_3:49621835-49621854 Msa0645150:CDS 45.0%
TCATGGCATGGATCATGGAA+TGG - chr4_3:49621840-49621859 Msa0645150:CDS 45.0%
CATGGATCATGGAATGGCAA+TGG - chr4_3:49621846-49621865 Msa0645150:CDS 45.0%
TACTTACCAGAACTACCAGC+AGG + chr4_3:49621985-49622004 None:intergenic 45.0%
CATGGCGAAAACAAGGATCA+AGG + chr4_3:49622016-49622035 None:intergenic 45.0%
GTATGGACATGGCGAAAACA+AGG + chr4_3:49622023-49622042 None:intergenic 45.0%
TGAACCGGGTTTAATGAACC+GGG + chr4_3:49622067-49622086 None:intergenic 45.0%
!! TAGGGTTGGCCTAGCTTATT+TGG - chr4_3:49622131-49622150 Msa0645150:CDS 45.0%
GCTCTCATGTCTTTCAATGG+TGG - chr4_3:49622162-49622181 Msa0645150:CDS 45.0%
! CTCTTTGACAATTGGCCTGC+TGG - chr4_3:49621967-49621986 Msa0645150:CDS 50.0%
! AATTGGCCTGCTGGTAGTTC+TGG - chr4_3:49621976-49621995 Msa0645150:CDS 50.0%
TCGAGTTTCTCTCAAGCACC+CGG - chr4_3:49622046-49622065 Msa0645150:CDS 50.0%
AGCACCCGGTTCATTAAACC+CGG - chr4_3:49622060-49622079 Msa0645150:CDS 50.0%
GTGTTCTTGGTTGCTGTGCT+TGG - chr4_3:49622186-49622205 Msa0645150:CDS 50.0%
CTGTGCTTGGTCATGCTGTT+GGG - chr4_3:49622199-49622218 Msa0645150:CDS 50.0%
ACCCGGTTCAAACCCTGTTG+TGG - chr4_3:49622077-49622096 Msa0645150:CDS 55.0%
GCCACAACAGGGTTTGAACC+GGG + chr4_3:49622081-49622100 None:intergenic 55.0%
GGCCACAACAGGGTTTGAAC+CGG + chr4_3:49622082-49622101 None:intergenic 55.0%
GGTTCAAACCCTGTTGTGGC+CGG - chr4_3:49622081-49622100 Msa0645150:CDS 55.0%
GAACTAGACCGGCCACAACA+GGG + chr4_3:49622092-49622111 None:intergenic 55.0%
TGAACTAGACCGGCCACAAC+AGG + chr4_3:49622093-49622112 None:intergenic 55.0%
GCTGTGCTTGGTCATGCTGT+TGG - chr4_3:49622198-49622217 Msa0645150:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 49621835 49622296 49621835 ID=Msa0645150;Name=Msa0645150
chr4_3 mRNA 49621835 49622296 49621835 ID=Msa0645150-mRNA-1;Parent=Msa0645150;Name=Msa0645150-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|153
chr4_3 exon 49621835 49622296 49621835 ID=Msa0645150-mRNA-1:exon:11859;Parent=Msa0645150-mRNA-1
chr4_3 CDS 49621835 49622296 49621835 ID=Msa0645150-mRNA-1:cds;Parent=Msa0645150-mRNA-1
Gene Sequence

>Msa0645150

ATGGATCATGGCATGGATCATGGAATGGCAATGGCACCAAAGTCATCATCATCTACCAATGACACTATGATGATGGGTCAGATGCATGACATGATGCACATGACATTCTTCTGCGGTAAAGATGCATTGATTCTCTTTGACAATTGGCCTGCTGGTAGTTCTGGTAAGTATGTATTGGCCTTGATCCTTGTTTTCGCCATGTCCATACTCATCGAGTTTCTCTCAAGCACCCGGTTCATTAAACCCGGTTCAAACCCTGTTGTGGCCGGTCTAGTTCAGACACTTTTGCATGTTCTTAGGGTTGGCCTAGCTTATTTGGTTATGTTGGCTCTCATGTCTTTCAATGGTGGAGTGTTCTTGGTTGCTGTGCTTGGTCATGCTGTTGGGTTCTTCTTTCGTAGCCGTGCTTTCAAAAAACCACATCAAGATGAGAATTTCGATCTTCCTCCGTTATCTTGTTAA

Protein sequence

>Msa0645150

MDHGMDHGMAMAPKSSSSTNDTMMMGQMHDMMHMTFFCGKDALILFDNWPAGSSGKYVLALILVFAMSILIEFLSSTRFIKPGSNPVVAGLVQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHAVGFFFRSRAFKKPHQDENFDLPPLSC*