AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0129850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129850 MtrunA17_Chr1g0197011 94.118 153 8 1 3 154 4 156 2.31e-98 280
Msa0129850 MtrunA17_Chr1g0177041 51.163 86 38 2 69 153 69 151 2.81e-26 97.4
Msa0129850 MtrunA17_Chr1g0197031 35.625 160 86 6 1 153 1 150 6.83e-24 91.3
Msa0129850 MtrunA17_Chr1g0197021 37.963 108 54 2 54 148 35 142 4.74e-21 84.0
Msa0129850 MtrunA17_Chr3g0081611 29.299 157 98 4 7 153 3 156 4.65e-19 79.0
Msa0129850 MtrunA17_Chr5g0426881 34.266 143 82 6 10 147 50 185 9.11e-19 81.6
Msa0129850 MtrunA17_Chr1g0196991 73.171 41 11 0 109 149 120 160 1.14e-18 77.8
Msa0129850 MtrunA17_Chr7g0246761 38.017 121 60 5 32 147 22 132 1.30e-18 78.2
Msa0129850 MtrunA17_Chr3g0128771 32.886 149 85 6 5 149 26 163 2.42e-18 77.4
Msa0129850 MtrunA17_Chr6g0486651 36.000 125 68 4 25 147 47 161 3.46e-18 79.7
Msa0129850 MtrunA17_Chr7g0248831 40.541 74 44 0 80 153 83 156 6.85e-18 76.3
Msa0129850 MtrunA17_Chr1g0198201 31.746 126 84 2 25 149 8 132 1.30e-17 76.3
Msa0129850 MtrunA17_Chr2g0325141 46.154 65 31 1 87 147 69 133 2.83e-17 73.6
Msa0129850 MtrunA17_Chr4g0030831 26.667 150 87 3 18 147 8 154 3.03e-17 74.3
Msa0129850 MtrunA17_Chr5g0407431 30.282 142 85 4 8 148 20 148 5.84e-17 74.3
Msa0129850 MtrunA17_Chr3g0132491 35.156 128 67 6 25 147 62 178 7.04e-17 76.3
Msa0129850 MtrunA17_Chr6g0470751 47.619 63 32 1 86 147 106 168 1.23e-16 75.1
Msa0129850 MtrunA17_Chr8g0348151 29.252 147 86 3 21 153 20 162 1.60e-16 73.2
Msa0129850 MtrunA17_Chr2g0325161 43.662 71 36 1 81 147 95 165 2.40e-16 73.2
Msa0129850 MtrunA17_Chr5g0409211 29.452 146 91 5 12 153 5 142 2.98e-16 71.6
Msa0129850 MtrunA17_Chr8g0364881 31.655 139 84 5 20 149 2 138 3.11e-16 73.9
Msa0129850 MtrunA17_Chr1g0191601 32.800 125 75 4 25 147 52 169 4.06e-16 73.9
Msa0129850 MtrunA17_Chr1g0203541 42.647 68 36 1 83 147 111 178 4.50e-16 72.0
Msa0129850 MtrunA17_Chr8g0350741 34.127 126 72 2 27 147 12 131 7.21e-16 70.9
Msa0129850 MtrunA17_Chr5g0396601 30.952 126 82 2 24 147 270 392 7.92e-16 73.2
Msa0129850 MtrunA17_Chr7g0273051 30.667 150 86 7 2 147 27 162 8.50e-16 73.2
Msa0129850 MtrunA17_Chr8g0370171 29.496 139 84 4 10 147 15 140 1.07e-15 70.9
Msa0129850 MtrunA17_Chr8g0383921 29.221 154 99 3 1 153 42 186 1.34e-15 70.9
Msa0129850 MtrunA17_Chr2g0327791 37.647 85 44 2 66 147 99 177 1.44e-15 70.9
Msa0129850 MtrunA17_Chr5g0396611 28.667 150 96 4 3 147 239 382 1.72e-15 72.4
Msa0129850 MtrunA17_Chr5g0426871 41.791 67 36 2 83 147 114 179 1.78e-15 72.4
Msa0129850 MtrunA17_Chr2g0301871 44.928 69 35 2 87 153 64 131 1.84e-15 71.6
Msa0129850 MtrunA17_Chr8g0388231 31.452 124 78 2 26 149 265 381 2.04e-15 72.0
Msa0129850 MtrunA17_Chr7g0244851 31.373 153 92 5 2 153 15 155 3.28e-15 68.9
Msa0129850 MtrunA17_Chr3g0133001 52.000 50 24 0 98 147 149 198 3.52e-15 71.6
Msa0129850 MtrunA17_Chr6g0451031 34.921 126 63 5 22 147 5 111 4.90e-15 67.4
Msa0129850 MtrunA17_Chr7g0248841 55.814 43 19 0 105 147 104 146 9.39e-15 67.4
Msa0129850 MtrunA17_Chr3g0129671 32.237 152 81 6 5 148 42 179 9.98e-15 68.6
Msa0129850 MtrunA17_Chr5g0413841 28.986 138 83 5 25 149 51 186 1.25e-14 69.7
Msa0129850 MtrunA17_Chr6g0451051 33.333 123 61 3 25 147 7 108 1.31e-14 67.0
Msa0129850 MtrunA17_Chr5g0396591 29.134 127 85 2 23 147 159 282 1.48e-14 68.9
Msa0129850 MtrunA17_Chr8g0369571 32.374 139 81 5 17 153 11 138 1.55e-14 67.4
Msa0129850 MtrunA17_Chr5g0426781 28.571 168 92 6 2 147 35 196 1.64e-14 69.3
Msa0129850 MtrunA17_Chr3g0123631 29.839 124 73 4 26 147 6 117 1.77e-14 67.4
Msa0129850 MtrunA17_Chr7g0275601 30.882 136 82 4 22 153 29 156 1.90e-14 66.6
Msa0129850 MtrunA17_Chr8g0366561 28.777 139 89 4 22 153 55 190 2.50e-14 68.9
Msa0129850 MtrunA17_Chr5g0427431 34.524 84 46 2 64 147 128 202 2.82e-14 68.9
Msa0129850 MtrunA17_Chr5g0433861 28.676 136 77 3 21 147 24 148 3.35e-14 68.2
Msa0129850 MtrunA17_Chr3g0123641 42.857 63 33 2 87 147 56 117 4.01e-14 67.4
Msa0129850 MtrunA17_Chr4g0013561 44.118 68 35 1 84 151 92 156 4.08e-14 67.0
Msa0129850 MtrunA17_Chr8g0366521 27.673 159 92 4 1 147 1 148 4.91e-14 67.8
Msa0129850 MtrunA17_Chr8g0381731 39.655 58 35 0 90 147 159 216 5.81e-14 67.4
Msa0129850 MtrunA17_Chr5g0406031 37.313 67 42 0 82 148 166 232 5.85e-14 66.6
Msa0129850 MtrunA17_Chr3g0113811 27.465 142 90 4 13 149 17 150 6.19e-14 65.5
Msa0129850 MtrunA17_Chr5g0409201 48.000 50 26 0 104 153 92 141 9.91e-14 64.7
Msa0129850 MtrunA17_Chr6g0451161 40.580 69 38 2 80 147 135 201 1.16e-13 67.0
Msa0129850 MtrunA17_Chr8g0336881 40.278 72 42 1 83 153 82 153 1.48e-13 66.6
Msa0129850 MtrunA17_Chr8g0388201 38.356 73 45 0 82 154 324 396 1.91e-13 66.6
Msa0129850 MtrunA17_Chr4g0073141 51.111 45 22 0 103 147 142 186 2.32e-13 66.2
Msa0129850 MtrunA17_Chr5g0444161 48.837 43 22 0 105 147 185 227 2.99e-13 64.7
Msa0129850 MtrunA17_Chr8g0383931 41.176 68 39 1 86 153 123 189 4.52e-13 64.3
Msa0129850 MtrunA17_Chr3g0105441 37.209 86 51 2 67 149 91 176 4.52e-13 64.7
Msa0129850 MtrunA17_Chr6g0451041 49.057 53 27 0 95 147 8 60 4.53e-13 61.6
Msa0129850 MtrunA17_Chr2g0297011 51.111 45 22 0 104 148 101 145 4.54e-13 63.2
Msa0129850 MtrunA17_Chr8g0371291 44.000 50 28 0 99 148 174 223 4.60e-13 64.3
Msa0129850 MtrunA17_Chr2g0307411 36.111 72 43 1 79 147 71 142 4.65e-13 63.5
Msa0129850 MtrunA17_Chr8g0383901 41.176 68 39 1 86 153 113 179 4.80e-13 63.9
Msa0129850 MtrunA17_Chr7g0261091 25.000 136 90 3 25 154 20 149 4.88e-13 65.1
Msa0129850 MtrunA17_Chr1g0167811 36.508 63 38 1 89 149 105 167 6.54e-13 64.7
Msa0129850 MtrunA17_Chr8g0383871 38.889 72 43 1 82 153 117 187 6.66e-13 63.9
Msa0129850 MtrunA17_Chr5g0411081 39.655 58 35 0 95 152 138 195 6.98e-13 64.7
Msa0129850 MtrunA17_Chr5g0444141 48.837 43 22 0 105 147 220 262 7.74e-13 64.3
Msa0129850 MtrunA17_Chr7g0276591 46.154 52 27 1 103 154 99 149 7.75e-13 64.3
Msa0129850 MtrunA17_Chr5g0437361 35.294 68 44 0 82 149 80 147 1.05e-12 62.4
Msa0129850 MtrunA17_Chr3g0117821 36.923 65 41 0 83 147 63 127 1.14e-12 61.6
Msa0129850 MtrunA17_Chr1g0186661 38.462 52 32 0 98 149 124 175 1.48e-12 63.9
Msa0129850 MtrunA17_Chr2g0279431 47.826 46 24 0 102 147 158 203 1.55e-12 63.9
Msa0129850 MtrunA17_Chr8g0347101 32.432 74 50 0 80 153 289 362 1.56e-12 63.9
Msa0129850 MtrunA17_Chr3g0118061 34.921 63 41 0 85 147 84 146 1.60e-12 62.8
Msa0129850 MtrunA17_Chr4g0067591 39.216 51 31 0 97 147 96 146 2.11e-12 62.8
Msa0129850 MtrunA17_Chr3g0144241 52.273 44 21 0 105 148 104 147 2.31e-12 61.6
Msa0129850 MtrunA17_Chr7g0274721 39.344 61 34 1 87 147 78 135 2.92e-12 61.2
Msa0129850 MtrunA17_Chr8g0383881 26.316 152 93 3 12 153 36 178 3.24e-12 61.6
Msa0129850 MtrunA17_Chr3g0126611 35.294 68 44 0 82 149 308 375 4.19e-12 62.8
Msa0129850 MtrunA17_Chr8g0383941 26.797 153 97 4 2 153 44 182 4.37e-12 61.6
Msa0129850 MtrunA17_Chr4g0050041 51.163 43 21 0 105 147 152 194 4.57e-12 62.4
Msa0129850 MtrunA17_Chr1g0184171 42.000 50 29 0 105 154 130 179 4.61e-12 62.4
Msa0129850 MtrunA17_Chr4g0052671 51.163 43 21 0 105 147 106 148 6.10e-12 60.1
Msa0129850 MtrunA17_Chr8g0383861 29.710 138 90 5 17 153 57 188 7.75e-12 60.8
Msa0129850 MtrunA17_Chr8g0345361 47.826 46 24 0 102 147 114 159 1.52e-11 60.5
Msa0129850 MtrunA17_Chr7g0274731 38.983 59 33 1 89 147 101 156 1.66e-11 59.7
Msa0129850 MtrunA17_Chr4g0059281 44.000 50 28 0 98 147 125 174 1.90e-11 60.5
Msa0129850 MtrunA17_Chr3g0118071 36.066 61 39 0 87 147 86 146 2.15e-11 59.7
Msa0129850 MtrunA17_Chr2g0300331 31.298 131 74 5 24 147 90 211 2.64e-11 59.7
Msa0129850 MtrunA17_Chr5g0430781 32.558 86 53 1 62 147 72 152 3.25e-11 58.9
Msa0129850 MtrunA17_Chr2g0295571 40.385 52 31 0 96 147 110 161 4.12e-11 59.3
Msa0129850 MtrunA17_Chr7g0269491 30.120 83 56 1 67 147 54 136 4.64e-11 58.5
Msa0129850 MtrunA17_Chr4g0060981 37.143 70 44 0 79 148 148 217 4.85e-11 58.5
Msa0129850 MtrunA17_Chr7g0274711 32.812 64 42 1 85 147 75 138 9.03e-11 57.4
Msa0129850 MtrunA17_Chr8g0369691 41.538 65 35 3 84 147 425 487 9.74e-11 58.9
Msa0129850 MtrunA17_Chr7g0263911 50.000 48 24 0 104 151 589 636 9.82e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129850 AT5G06490.1 34.463 177 87 6 5 153 2 177 2.85e-25 96.3
Msa0129850 AT2G35910.1 51.351 74 34 1 80 153 124 195 1.37e-22 89.7
Msa0129850 AT4G10160.1 33.094 139 85 3 11 149 11 141 3.64e-19 80.9
Msa0129850 AT5G53110.1 34.146 123 75 2 27 149 258 374 2.64e-18 80.5
Msa0129850 AT2G46493.1 27.200 125 89 1 25 149 60 182 3.33e-18 77.4
Msa0129850 AT1G23980.1 31.343 134 79 4 25 147 55 186 1.99e-17 78.2
Msa0129850 AT2G46160.1 44.444 72 34 1 86 151 113 184 6.23e-17 74.7
Msa0129850 AT2G46160.2 44.444 72 34 1 86 151 113 184 6.23e-17 74.7
Msa0129850 AT1G76410.1 32.031 128 82 3 22 148 24 147 1.32e-16 73.2
Msa0129850 AT4G28890.1 32.215 149 85 5 10 147 22 165 1.46e-16 75.9
Msa0129850 AT1G28040.1 29.457 129 83 2 26 149 172 297 1.77e-16 74.7
Msa0129850 AT2G35000.1 30.709 127 79 2 22 147 59 177 4.47e-16 74.3
Msa0129850 AT1G20823.1 34.400 125 69 5 26 148 41 154 6.37e-16 71.6
Msa0129850 AT4G10150.1 33.835 133 72 6 24 149 32 155 7.36e-16 72.4
Msa0129850 AT2G25410.1 30.070 143 87 4 14 147 231 369 9.79e-16 73.2
Msa0129850 AT3G61550.1 47.368 57 30 0 97 153 128 184 1.07e-15 71.2
Msa0129850 AT2G34990.1 26.923 130 93 2 19 147 10 138 1.51e-15 72.4
Msa0129850 AT2G34990.2 26.923 130 93 2 19 147 32 160 1.69e-15 72.4
Msa0129850 AT5G27420.1 38.372 86 47 3 64 147 85 166 2.12e-15 72.4
Msa0129850 AT1G35330.1 43.939 66 34 2 86 149 109 173 2.20e-15 72.0
Msa0129850 AT4G40070.1 30.405 148 91 4 10 154 35 173 2.68e-15 71.6
Msa0129850 AT3G05200.1 45.455 66 33 2 84 147 106 170 2.73e-15 72.0
Msa0129850 AT5G05280.1 32.026 153 86 5 4 151 20 159 3.81e-15 69.3
Msa0129850 AT2G20030.1 31.343 134 71 6 23 147 45 166 4.53e-15 71.6
Msa0129850 AT5G07040.1 29.252 147 95 5 8 153 4 142 6.33e-15 68.2
Msa0129850 AT4G17905.2 42.857 70 37 1 88 154 161 230 1.05e-14 70.1
Msa0129850 AT4G17905.1 42.857 70 37 1 88 154 135 204 1.14e-14 70.1
Msa0129850 AT1G04360.1 30.597 134 78 3 25 149 51 178 1.81e-14 69.7
Msa0129850 AT1G72200.1 40.000 65 38 1 84 147 122 186 1.83e-14 69.7
Msa0129850 AT4G09120.1 34.940 83 51 2 67 147 84 165 1.96e-14 69.7
Msa0129850 AT3G48030.2 39.130 69 41 1 81 149 106 173 2.79e-14 68.6
Msa0129850 AT1G72310.1 39.773 88 43 3 67 152 95 174 3.10e-14 68.9
Msa0129850 AT3G03550.1 44.828 58 32 0 90 147 144 201 3.66e-14 68.9
Msa0129850 AT3G48030.1 39.130 69 41 1 81 149 184 251 3.67e-14 68.9
Msa0129850 AT5G05810.1 49.020 51 26 0 97 147 138 188 4.38e-14 68.9
Msa0129850 AT2G46494.1 29.688 128 75 3 33 149 237 360 5.98e-14 68.2
Msa0129850 AT2G47560.1 44.262 61 34 0 87 147 90 150 6.45e-14 67.0
Msa0129850 AT3G62690.1 43.284 67 36 1 81 147 91 155 6.63e-14 67.4
Msa0129850 AT4G09130.1 39.706 68 34 2 84 147 98 162 7.16e-14 68.2
Msa0129850 AT3G10910.1 50.000 50 25 0 102 151 111 160 7.29e-14 65.9
Msa0129850 AT4G33565.1 45.283 53 29 0 95 147 207 259 8.31e-14 67.8
Msa0129850 AT2G18650.1 48.077 52 27 0 101 152 122 173 9.74e-14 67.8
Msa0129850 AT4G09110.1 33.858 127 71 6 23 147 50 165 1.22e-13 67.0
Msa0129850 AT5G17600.1 43.103 58 33 0 90 147 127 184 1.52e-13 67.0
Msa0129850 AT5G01880.1 46.939 49 26 0 101 149 101 149 1.67e-13 64.7
Msa0129850 AT1G22500.1 32.283 127 79 5 23 147 39 160 2.11e-13 66.6
Msa0129850 AT5G66070.5 35.802 81 45 2 68 148 146 219 2.27e-13 65.5
Msa0129850 AT5G66070.1 35.802 81 45 2 68 148 146 219 2.27e-13 65.5
Msa0129850 AT5G66070.2 35.802 81 45 2 68 148 170 243 2.45e-13 65.5
Msa0129850 AT2G46495.1 28.378 148 87 6 11 149 227 364 2.56e-13 66.6
Msa0129850 AT3G18773.1 31.884 138 82 5 13 147 44 172 2.81e-13 65.1
Msa0129850 AT4G30400.1 51.111 45 22 0 103 147 132 176 2.97e-13 66.6
Msa0129850 AT5G57750.1 30.000 130 77 3 27 147 40 164 3.17e-13 64.7
Msa0129850 AT2G42360.1 51.020 49 24 0 99 147 102 150 3.26e-13 65.1
Msa0129850 AT3G20395.1 53.191 47 22 0 101 147 167 213 3.63e-13 64.7
Msa0129850 AT3G16720.1 51.111 45 22 0 103 147 117 161 3.70e-13 65.9
Msa0129850 AT2G42350.1 46.154 52 28 0 102 153 97 148 4.08e-13 64.7
Msa0129850 AT2G17450.1 30.070 143 85 4 10 148 14 145 4.33e-13 63.9
Msa0129850 AT5G10380.1 47.826 46 24 0 102 147 132 177 4.39e-13 65.5
Msa0129850 AT2G27940.1 40.299 67 37 1 87 153 125 188 4.58e-13 64.7
Msa0129850 AT1G71980.1 44.444 72 37 3 84 154 212 281 4.68e-13 65.9
Msa0129850 AT4G35840.1 29.457 129 81 3 30 148 106 234 4.81e-13 64.7
Msa0129850 AT4G09100.1 37.313 67 41 1 82 147 61 127 5.36e-13 62.4
Msa0129850 AT4G35480.1 30.000 150 96 4 6 148 9 156 5.60e-13 63.9
Msa0129850 AT1G33480.1 53.488 43 20 0 105 147 100 142 6.42e-13 64.7
Msa0129850 AT1G74410.1 51.111 45 22 0 105 149 176 220 9.63e-13 63.5
Msa0129850 AT5G36001.1 38.462 65 36 1 89 149 244 308 9.90e-13 64.7
Msa0129850 AT1G49210.1 32.117 137 83 5 12 147 50 177 1.38e-12 63.2
Msa0129850 AT1G72220.1 48.837 43 22 0 105 147 177 219 1.40e-12 64.3
Msa0129850 AT2G17730.1 35.526 76 48 1 74 148 164 239 1.42e-12 63.5
Msa0129850 AT4G15975.1 45.652 46 25 0 102 147 73 118 1.57e-12 63.2
Msa0129850 AT1G49220.1 32.117 137 83 5 12 147 50 177 1.73e-12 63.5
Msa0129850 AT2G44578.1 53.488 43 20 0 105 147 70 112 2.29e-12 61.2
Msa0129850 AT1G35630.1 32.800 125 67 6 29 148 164 276 2.32e-12 63.5
Msa0129850 AT3G14320.1 48.936 47 24 0 102 148 85 131 2.37e-12 62.4
Msa0129850 AT1G53820.1 45.833 48 26 0 102 149 117 164 2.64e-12 63.2
Msa0129850 AT5G40250.1 51.111 45 22 0 103 147 141 185 3.06e-12 63.5
Msa0129850 AT1G49230.1 31.159 138 78 6 14 147 49 173 3.55e-12 62.0
Msa0129850 AT1G49200.1 31.655 139 81 6 12 147 51 178 4.45e-12 62.0
Msa0129850 AT5G58580.1 53.061 49 23 0 105 153 138 186 6.05e-12 62.4
Msa0129850 AT3G18930.2 33.735 83 47 1 80 154 123 205 6.75e-12 62.4
Msa0129850 AT3G18930.3 33.735 83 47 1 80 154 123 205 6.75e-12 62.4
Msa0129850 AT3G18930.1 33.735 83 47 1 80 154 123 205 6.75e-12 62.4
Msa0129850 AT3G60220.1 37.500 72 44 1 83 153 94 165 7.02e-12 62.4
Msa0129850 AT1G35625.1 36.364 77 46 3 73 148 202 276 8.31e-12 62.0
Msa0129850 AT5G43420.1 43.182 44 25 0 104 147 137 180 8.60e-12 62.0
Msa0129850 AT2G46495.2 37.313 67 41 1 84 149 270 336 8.79e-12 62.0
Msa0129850 AT1G32361.1 37.313 67 38 2 83 149 136 198 9.92e-12 62.0
Msa0129850 AT4G38140.1 47.170 53 27 1 103 154 60 112 1.01e-11 59.7
Msa0129850 AT2G35420.1 40.323 62 35 1 86 147 86 145 1.03e-11 61.2
Msa0129850 AT5G37270.1 40.278 72 38 2 82 148 127 198 1.88e-11 60.1
Msa0129850 AT5G37250.2 40.278 72 38 2 82 148 127 198 2.01e-11 60.1
Msa0129850 AT1G80400.1 30.303 132 70 5 30 147 273 396 2.22e-11 60.8
Msa0129850 AT5G37250.1 40.278 72 38 2 82 148 111 182 2.24e-11 59.7
Msa0129850 AT3G55530.1 31.522 92 60 2 58 147 162 252 2.58e-11 60.5
Msa0129850 AT1G22670.1 51.852 54 24 2 96 148 223 275 2.86e-11 60.8
Msa0129850 AT1G53010.1 37.037 81 45 2 72 148 95 173 3.90e-11 58.5
Msa0129850 AT5G37230.1 38.889 72 39 2 82 148 127 198 4.08e-11 58.9
Msa0129850 AT3G11110.1 51.163 43 21 0 107 149 109 151 8.19e-11 57.4
Msa0129850 AT4G26400.2 45.833 48 25 1 100 147 236 282 9.01e-11 59.3
Msa0129850 AT4G26400.1 45.833 48 25 1 100 147 236 282 9.01e-11 59.3

Find 23 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATCTTAATACTTGTATT+TGG 0.241998 1_3:-59922465 Msa0129850:CDS
TGTATTTGGTCTCGTATTCT+TGG 0.251746 1_3:-59922451 Msa0129850:CDS
TCTGAGAGTATAAAAGTTTC+GGG 0.311980 1_3:+59922277 None:intergenic
ATCTGAGAGTATAAAAGTTT+CGG 0.329103 1_3:+59922276 None:intergenic
ATTAGTTGCTCTATTTGCTT+AGG 0.404219 1_3:-59922228 Msa0129850:CDS
TACTCTCAGATTCAGAAGAA+TGG 0.427690 1_3:-59922264 Msa0129850:CDS
ATGACGATGTGGTGGAAAAC+CGG 0.430831 1_3:+59922359 None:intergenic
TGATAAAGATGACCACAATC+TGG 0.456707 1_3:+59922171 None:intergenic
ATGTTGCTTGTGTTGATCCA+TGG 0.521110 1_3:-59922149 Msa0129850:CDS
TTGAGGTTACTTCCAGATTG+TGG 0.535101 1_3:-59922183 Msa0129850:CDS
GTTGTTGTGTGGAGGTTCCA+AGG 0.543708 1_3:+59922515 None:intergenic
TCGTCATGAAGACTCAATCA+TGG 0.545902 1_3:-59922343 Msa0129850:CDS
ATAAAGAAAGTGACATGTTG+AGG 0.576166 1_3:-59922200 Msa0129850:CDS
TCAAAACCGTTGTTGTTGTG+TGG 0.584708 1_3:+59922504 None:intergenic
GAGCAACAACAACGGCACCT+TGG 0.585236 1_3:-59922532 Msa0129850:CDS
GTGGAATGTAACATGAGCCA+TGG 0.599564 1_3:+59922132 None:intergenic
AAACCGTTGTTGTTGTGTGG+AGG 0.603017 1_3:+59922507 None:intergenic
GTATCCTTCAAAACTTGTGT+CGG 0.641573 1_3:+59922299 None:intergenic
GAGTCTTCATGACGATGTGG+TGG 0.660756 1_3:+59922351 None:intergenic
GAAGACTCAATCATGGAACA+AGG 0.664922 1_3:-59922336 Msa0129850:CDS
GAACCTCCACACAACAACAA+CGG 0.665355 1_3:-59922510 Msa0129850:CDS
ATTGAGTCTTCATGACGATG+TGG 0.716521 1_3:+59922348 None:intergenic
AAGAAGATGAGCAACAACAA+CGG 0.717539 1_3:-59922540 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATCTTAATACTTGTATT+TGG - chr1_3:59922161-59922180 Msa0129850:CDS 15.0%
!! AACATTCTCAATATTTTGTC+CGG - chr1_3:59922248-59922267 Msa0129850:CDS 25.0%
!! ATCTGAGAGTATAAAAGTTT+CGG + chr1_3:59922353-59922372 None:intergenic 25.0%
!! AGTGATGATTTTCTACATAT+GGG + chr1_3:59922524-59922543 None:intergenic 25.0%
!! TAGTGATGATTTTCTACATA+TGG + chr1_3:59922525-59922544 None:intergenic 25.0%
! TCTGAGAGTATAAAAGTTTC+GGG + chr1_3:59922352-59922371 None:intergenic 30.0%
ATTAGTTGCTCTATTTGCTT+AGG - chr1_3:59922398-59922417 Msa0129850:CDS 30.0%
ATAAAGAAAGTGACATGTTG+AGG - chr1_3:59922426-59922445 Msa0129850:CDS 30.0%
TGTATTTGGTCTCGTATTCT+TGG - chr1_3:59922175-59922194 Msa0129850:CDS 35.0%
GTATCCTTCAAAACTTGTGT+CGG + chr1_3:59922330-59922349 None:intergenic 35.0%
TACTCTCAGATTCAGAAGAA+TGG - chr1_3:59922362-59922381 Msa0129850:CDS 35.0%
TGATAAAGATGACCACAATC+TGG + chr1_3:59922458-59922477 None:intergenic 35.0%
! TTTTCTACATATGGGACAAG+TGG + chr1_3:59922516-59922535 None:intergenic 35.0%
! TCAAAACCGTTGTTGTTGTG+TGG + chr1_3:59922125-59922144 None:intergenic 40.0%
!! AACAACGGTTTTGACGCAAA+TGG - chr1_3:59922131-59922150 Msa0129850:CDS 40.0%
!! ACAACGGTTTTGACGCAAAT+GGG - chr1_3:59922132-59922151 Msa0129850:CDS 40.0%
ATTGAGTCTTCATGACGATG+TGG + chr1_3:59922281-59922300 None:intergenic 40.0%
TCGTCATGAAGACTCAATCA+TGG - chr1_3:59922283-59922302 Msa0129850:CDS 40.0%
GAAGACTCAATCATGGAACA+AGG - chr1_3:59922290-59922309 Msa0129850:CDS 40.0%
!! CATACCGACACAAGTTTTGA+AGG - chr1_3:59922323-59922342 Msa0129850:CDS 40.0%
TTGAGGTTACTTCCAGATTG+TGG - chr1_3:59922443-59922462 Msa0129850:CDS 40.0%
! ATGTTGCTTGTGTTGATCCA+TGG - chr1_3:59922477-59922496 Msa0129850:CDS 40.0%
GAACCTCCACACAACAACAA+CGG - chr1_3:59922116-59922135 Msa0129850:CDS 45.0%
! AAACCGTTGTTGTTGTGTGG+AGG + chr1_3:59922122-59922141 None:intergenic 45.0%
! ATGACGATGTGGTGGAAAAC+CGG + chr1_3:59922270-59922289 None:intergenic 45.0%
GTGGAATGTAACATGAGCCA+TGG + chr1_3:59922497-59922516 None:intergenic 45.0%
GTTGTTGTGTGGAGGTTCCA+AGG + chr1_3:59922114-59922133 None:intergenic 50.0%
GAGTCTTCATGACGATGTGG+TGG + chr1_3:59922278-59922297 None:intergenic 50.0%
GAGCAACAACAACGGCACCT+TGG - chr1_3:59922094-59922113 Msa0129850:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 59922092 59922556 59922092 ID=Msa0129850;Name=Msa0129850
chr1_3 mRNA 59922092 59922556 59922092 ID=Msa0129850-mRNA-1;Parent=Msa0129850;Name=Msa0129850-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|154
chr1_3 exon 59922092 59922556 59922092 ID=Msa0129850-mRNA-1:exon:19655;Parent=Msa0129850-mRNA-1
chr1_3 CDS 59922092 59922556 59922092 ID=Msa0129850-mRNA-1:cds;Parent=Msa0129850-mRNA-1
Gene Sequence

>Msa0129850

ATGAGCAACAACAACGGCACCTTGGAACCTCCACACAACAACAACGGTTTTGACGCAAATGGGTTCACATATATCTTAATACTTGTATTTGGTCTCGTATTCTTGGTTCTATCAATAATTGTTGCTTGTATTCGACTTCGTAGTTCACAAAGTCGAAACATTCTCAATATTTTGTCCGGTTTTCCACCACATCGTCATGAAGACTCAATCATGGAACAAGGACTTCGTCATCATACCGACACAAGTTTTGAAGGATACCCGAAACTTTTATACTCTCAGATTCAGAAGAATGGTTCTTCAACTGCAATTAGTTGCTCTATTTGCTTAGGAGACTATAAAGAAAGTGACATGTTGAGGTTACTTCCAGATTGTGGTCATCTTTATCATGTTGCTTGTGTTGATCCATGGCTCATGTTACATTCCACTTGTCCCATATGTAGAAAATCATCACTAGCTTCAACCTAG
Protein sequence

>Msa0129850

MSNNNGTLEPPHNNNGFDANGFTYILILVFGLVFLVLSIIVACIRLRSSQSRNILNILSGFPPHRHEDSIMEQGLRHHTDTSFEGYPKLLYSQIQKNGSSTAISCSICLGDYKESDMLRLLPDCGHLYHVACVDPWLMLHSTCPICRKSSLAST*