AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0038090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038090 MtrunA17_Chr1g0201521 100.000 152 0 0 1 152 1 152 5.12e-112 314
Msa0038090 MtrunA17_Chr1g0201481 100.000 152 0 0 1 152 1 152 5.12e-112 314
Msa0038090 MtrunA17_Chr6g0467091 98.684 152 2 0 1 152 1 152 5.31e-111 311
Msa0038090 MtrunA17_Chr5g0409931 94.286 105 6 0 36 140 12 116 2.72e-70 207
Msa0038090 MtrunA17_Chr5g0409851 93.258 89 6 0 52 140 1 89 3.41e-58 176
Msa0038090 MtrunA17_Chr5g0409921 94.382 89 5 0 52 140 1 89 4.30e-58 175
Msa0038090 MtrunA17_Chr5g0409871 94.382 89 5 0 52 140 1 89 5.53e-58 175
Msa0038090 MtrunA17_Chr5g0409881 94.382 89 5 0 52 140 1 89 5.53e-58 175
Msa0038090 MtrunA17_Chr5g0409861 94.382 89 5 0 52 140 1 89 5.53e-58 175
Msa0038090 MtrunA17_Chr5g0409911 94.382 89 5 0 52 140 1 89 5.53e-58 175
Msa0038090 MtrunA17_Chr5g0409891 94.382 89 5 0 52 140 1 89 5.53e-58 175
Msa0038090 MtrunA17_Chr5g0409901 95.714 70 3 0 52 121 1 70 1.12e-43 138
Msa0038090 MtrunA17_Chr4g0006461 76.562 64 14 1 67 129 9 72 3.44e-28 99.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0038090 AT4G09800.1 92.763 152 11 0 1 152 1 152 1.97e-105 298
Msa0038090 AT1G34030.1 92.763 152 11 0 1 152 1 152 1.97e-105 298
Msa0038090 AT1G22780.1 92.763 152 11 0 1 152 1 152 1.97e-105 298

Find 50 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAGTTGAGAGATGATT+TGG 0.279609 1_1:+67339159 Msa0038090:CDS
CAACTTCATGTCGAGTTGAT+TGG 0.309472 1_1:-67339146 None:intergenic
CGTCCGCAGTTAGGTTAAAA+TGG 0.345502 1_1:+67338597 Msa0038090:exon
CTTAAGGCAGTATGTCGCTC+TGG 0.360105 1_1:-67338544 None:intergenic
ACCGGGGTTTGAGGCACTAC+TGG 0.376368 1_1:+67340159 Msa0038090:CDS
TTAAGCGTGATGTCCTGTTT+TGG 0.377769 1_1:+67340256 Msa0038090:exon
TCCCCTGCGGCCTGTAGTCT+TGG 0.394682 1_1:-67340207 None:intergenic
TTTCATATACCGAGTTTGTA+TGG 0.407920 1_1:+67340322 Msa0038090:three_prime_UTR
CCGGGGTTTGAGGCACTACT+GGG 0.411270 1_1:+67340160 Msa0038090:CDS
AAAATCCTCCGTCCGCAGTT+AGG 0.418193 1_1:+67338588 Msa0038090:five_prime_UTR
AATCATCTAATCTCCAAAAC+AGG 0.431449 1_1:-67340269 None:intergenic
TTCTGTTATGCAGTAATCAC+CGG 0.438670 1_1:+67340141 Msa0038090:intron
ATACCACCATTCAACTAATC+AGG 0.439185 1_1:-67340400 None:intergenic
TACTGGGGCCTTCGTGTTCG+TGG 0.449083 1_1:+67340176 Msa0038090:CDS
GTGTTGAACACAAATGTTGA+TGG 0.456259 1_1:+67338738 Msa0038090:CDS
TGATTGAGGTCATTGCGAAC+TGG 0.463060 1_1:-67338773 None:intergenic
AGAGGGGTAATTGCCGCTTA+AGG 0.472446 1_1:-67338560 None:intergenic
GTTCGCAATGACCTCAATCA+AGG 0.484324 1_1:+67338776 Msa0038090:CDS
CAAGGATGGCAAATTCTCTC+AGG 0.484690 1_1:+67339117 Msa0038090:CDS
ATGACCTCAATCAAGGGTAT+TGG 0.493197 1_1:+67338783 Msa0038090:CDS
GTCAATTCAAGGTTCCAGAT+TGG 0.495981 1_1:+67339071 Msa0038090:CDS
CAAGTGCCTGATTAGTTGAA+TGG 0.501343 1_1:+67340394 Msa0038090:three_prime_UTR
CCCAGTAGTGCCTCAAACCC+CGG 0.505879 1_1:-67340160 None:intergenic
TGGAGAGACTCAAGAAGATC+CGG 0.512286 1_1:+67339179 Msa0038090:CDS
TGCAAATCCTCGTCAATTCA+AGG 0.522315 1_1:+67339060 Msa0038090:CDS
GAACAGAAAGAAGGATTACA+AGG 0.525479 1_1:+67339099 Msa0038090:CDS
GCAGTAATCACCGGGGTTTG+AGG 0.529690 1_1:+67340150 Msa0038090:intron
TTAAGGCAGTATGTCGCTCT+GGG 0.543045 1_1:-67338543 None:intergenic
TGATGTGTTATAGTCTTTGG+TGG 0.546379 1_1:+67338686 Msa0038090:intron
TGAATTGAGTGCTGCAGAGT+TGG 0.553099 1_1:+67339018 Msa0038090:CDS
AGAAAGAAGGATTACAAGGA+TGG 0.565673 1_1:+67339103 Msa0038090:CDS
GGTCAGCATACCAAGACTAC+AGG 0.569467 1_1:+67340197 Msa0038090:CDS
TACCAAGACTACAGGCCGCA+GGG 0.580402 1_1:+67340205 Msa0038090:CDS
CCTACCAATACCCTTGATTG+AGG 0.590405 1_1:-67338787 None:intergenic
ATACCAAGACTACAGGCCGC+AGG 0.594524 1_1:+67340204 Msa0038090:CDS
TCTGTTATGCAGTAATCACC+GGG 0.594587 1_1:+67340142 Msa0038090:intron
CTGATGTTGACATGAACAAG+AGG 0.595729 1_1:+67338838 Msa0038090:CDS
GAGCGACATACTGCCTTAAG+CGG 0.600119 1_1:+67338547 Msa0038090:five_prime_UTR
GGCCGCAGGGGAAAGACTGT+TGG 0.608087 1_1:+67340218 Msa0038090:CDS
GTTGGATAATATTATGACAG+TGG 0.620536 1_1:+67339036 Msa0038090:CDS
TTCGCAATGACCTCAATCAA+GGG 0.622158 1_1:+67338777 Msa0038090:CDS
CCTCAATCAAGGGTATTGGT+AGG 0.631523 1_1:+67338787 Msa0038090:CDS
TTTAACCTAACTGCGGACGG+AGG 0.653943 1_1:-67338593 None:intergenic
ACCAAGACTACAGGCCGCAG+GGG 0.654823 1_1:+67340206 Msa0038090:CDS
TCTGGAACCTTGAATTGACG+AGG 0.669518 1_1:-67339067 None:intergenic
CGGGGTTTGAGGCACTACTG+GGG 0.682879 1_1:+67340161 Msa0038090:CDS
GTGCCTGATTAGTTGAATGG+TGG 0.720987 1_1:+67340397 Msa0038090:three_prime_UTR
CTGTTATGCAGTAATCACCG+GGG 0.730953 1_1:+67340143 Msa0038090:intron
TATGCTGACCACGAACACGA+AGG 0.739225 1_1:-67340184 None:intergenic
CACCAACAGTCTTTCCCCTG+CGG 0.740988 1_1:-67340220 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAATTCAATAATATAA+TGG + chr1_1:67340064-67340083 Msa0038090:intron 10.0%
!! AATTCAATTCAATAATATAA+TGG + chr1_1:67340064-67340083 Msa0038090:intron 10.0%
!!! ATTTTTGTTGTTCAAATTAA+TGG + chr1_1:67338898-67338917 Msa0038090:intron 15.0%
!!! TTTTTGTTGTTCAAATTAAT+GGG + chr1_1:67338899-67338918 Msa0038090:intron 15.0%
!!! GGTTTTTTTTTTTTTTTTGT+TGG + chr1_1:67338979-67338998 Msa0038090:intron 15.0%
!!! TTTTTTTGTTGGATTTATTT+AGG + chr1_1:67338990-67339009 Msa0038090:intron 15.0%
!!! TTTTTTGTTGGATTTATTTA+GGG + chr1_1:67338991-67339010 Msa0038090:intron 15.0%
!! TTTGAGTAGTAAAAAAATAA+TGG - chr1_1:67339987-67340006 None:intergenic 15.0%
!!! ATTTTTGTTGTTCAAATTAA+TGG + chr1_1:67338898-67338917 Msa0038090:intron 15.0%
!!! TTTTTGTTGTTCAAATTAAT+GGG + chr1_1:67338899-67338918 Msa0038090:intron 15.0%
!!! GGTTTTTTTTTTTTTTTTGT+TGG + chr1_1:67338979-67338998 Msa0038090:intron 15.0%
!!! TTTTTTTGTTGGATTTATTT+AGG + chr1_1:67338990-67339009 Msa0038090:intron 15.0%
!!! TTTTTTGTTGGATTTATTTA+GGG + chr1_1:67338991-67339010 Msa0038090:intron 15.0%
!! TTTGAGTAGTAAAAAAATAA+TGG - chr1_1:67339987-67340006 None:intergenic 15.0%
!!! TATTTTTGATGTTGTTGAAA+TGG + chr1_1:67338958-67338977 Msa0038090:intron 20.0%
!! TATGATATTCATGTTGATAT+AGG + chr1_1:67339730-67339749 Msa0038090:intron 20.0%
!! ATGATATTCATGTTGATATA+GGG + chr1_1:67339731-67339750 Msa0038090:intron 20.0%
!!! CAATAAAATTTTAGACTGTT+TGG + chr1_1:67339781-67339800 Msa0038090:intron 20.0%
!! ACAGTTTAATTAAGCATTTA+TGG + chr1_1:67339808-67339827 Msa0038090:intron 20.0%
!! AATAAGATGAAAACAGTTTA+TGG + chr1_1:67339877-67339896 Msa0038090:intron 20.0%
!! TAGTTTAAATAAATCCTATC+TGG + chr1_1:67339956-67339975 Msa0038090:intron 20.0%
!! AGTTTAAATAAATCCTATCT+GGG + chr1_1:67339957-67339976 Msa0038090:intron 20.0%
!! TTCTGTATTCAATAATACAA+TGG + chr1_1:67340007-67340026 Msa0038090:intron 20.0%
!!! AATAATATAATGGATGTATG+TGG + chr1_1:67340074-67340093 Msa0038090:intron 20.0%
!! AATTAAAAACCATACAAACT+CGG - chr1_1:67340334-67340353 None:intergenic 20.0%
!!! CAGATTTTTTAACTTTCTAA+TGG + chr1_1:67340458-67340477 Msa0038090:intron 20.0%
!!! TATTTTTGATGTTGTTGAAA+TGG + chr1_1:67338958-67338977 Msa0038090:intron 20.0%
!! TATGATATTCATGTTGATAT+AGG + chr1_1:67339730-67339749 Msa0038090:intron 20.0%
!! ATGATATTCATGTTGATATA+GGG + chr1_1:67339731-67339750 Msa0038090:intron 20.0%
!!! CAATAAAATTTTAGACTGTT+TGG + chr1_1:67339781-67339800 Msa0038090:intron 20.0%
!! ACAGTTTAATTAAGCATTTA+TGG + chr1_1:67339808-67339827 Msa0038090:intron 20.0%
!! AATAAGATGAAAACAGTTTA+TGG + chr1_1:67339877-67339896 Msa0038090:intron 20.0%
!! TAGTTTAAATAAATCCTATC+TGG + chr1_1:67339956-67339975 Msa0038090:intron 20.0%
!! AGTTTAAATAAATCCTATCT+GGG + chr1_1:67339957-67339976 Msa0038090:intron 20.0%
!! TTCTGTATTCAATAATACAA+TGG + chr1_1:67340007-67340026 Msa0038090:intron 20.0%
!!! AATAATATAATGGATGTATG+TGG + chr1_1:67340074-67340093 Msa0038090:intron 20.0%
!! AATTAAAAACCATACAAACT+CGG - chr1_1:67340334-67340353 None:intergenic 20.0%
!!! CAGATTTTTTAACTTTCTAA+TGG + chr1_1:67340458-67340477 Msa0038090:intron 20.0%
!! AGAATGCGAAAGAAATTTTA+GGG - chr1_1:67338517-67338536 None:intergenic 25.0%
!! TATGATTTTTAAACGTGAGA+TGG - chr1_1:67338940-67338959 None:intergenic 25.0%
! ATCATCTTTATAGTTAACAC+AGG - chr1_1:67339242-67339261 None:intergenic 25.0%
!!! ACATTTTAACACTACTATGT+AGG + chr1_1:67339299-67339318 Msa0038090:intron 25.0%
! GAAAACAAAATACAAGTCAT+TGG - chr1_1:67339643-67339662 None:intergenic 25.0%
!!! ACTTGTATTTTGTTTTCTCT+TGG + chr1_1:67339646-67339665 Msa0038090:intron 25.0%
! AATACATAGTAAAAGATGCA+AGG - chr1_1:67339694-67339713 None:intergenic 25.0%
! AATAATACAATGGATGTATG+TGG + chr1_1:67340017-67340036 Msa0038090:intron 25.0%
!!! TGGTTTTTAATTGAATGCAT+TGG + chr1_1:67340342-67340361 Msa0038090:three_prime_UTR 25.0%
!! TTGGCAATTTCAATTTTGTT+TGG + chr1_1:67340361-67340380 Msa0038090:three_prime_UTR 25.0%
! AAAAAATCTGAAGAGCAAAT+TGG - chr1_1:67340448-67340467 None:intergenic 25.0%
!! AGAATGCGAAAGAAATTTTA+GGG - chr1_1:67338517-67338536 None:intergenic 25.0%
!! TATGATTTTTAAACGTGAGA+TGG - chr1_1:67338940-67338959 None:intergenic 25.0%
! ATCATCTTTATAGTTAACAC+AGG - chr1_1:67339242-67339261 None:intergenic 25.0%
!!! ACATTTTAACACTACTATGT+AGG + chr1_1:67339299-67339318 Msa0038090:intron 25.0%
! GAAAACAAAATACAAGTCAT+TGG - chr1_1:67339643-67339662 None:intergenic 25.0%
!!! ACTTGTATTTTGTTTTCTCT+TGG + chr1_1:67339646-67339665 Msa0038090:intron 25.0%
! AATACATAGTAAAAGATGCA+AGG - chr1_1:67339694-67339713 None:intergenic 25.0%
! AATAATACAATGGATGTATG+TGG + chr1_1:67340017-67340036 Msa0038090:intron 25.0%
!!! TGGTTTTTAATTGAATGCAT+TGG + chr1_1:67340342-67340361 Msa0038090:three_prime_UTR 25.0%
!! TTGGCAATTTCAATTTTGTT+TGG + chr1_1:67340361-67340380 Msa0038090:three_prime_UTR 25.0%
! AAAAAATCTGAAGAGCAAAT+TGG - chr1_1:67340448-67340467 None:intergenic 25.0%
!!! ATTTTAGGGTTTGTGAGAAT+GGG - chr1_1:67338503-67338522 None:intergenic 30.0%
!!! AATTTTAGGGTTTGTGAGAA+TGG - chr1_1:67338504-67338523 None:intergenic 30.0%
! GAGAATGCGAAAGAAATTTT+AGG - chr1_1:67338518-67338537 None:intergenic 30.0%
!! ATACCATTTTAACCTAACTG+CGG - chr1_1:67338603-67338622 None:intergenic 30.0%
TTGTGATGTGTTATAGTCTT+TGG + chr1_1:67338683-67338702 Msa0038090:intron 30.0%
GTTGGATAATATTATGACAG+TGG + chr1_1:67339036-67339055 Msa0038090:CDS 30.0%
TTTCTGTTCAAAAACCAATC+TGG - chr1_1:67339088-67339107 None:intergenic 30.0%
!! TTGGTTTTTGAACAGAAAGA+AGG + chr1_1:67339090-67339109 Msa0038090:CDS 30.0%
!!! CTCAGTTGGTTTTTTAGTAT+AGG + chr1_1:67339267-67339286 Msa0038090:intron 30.0%
AGTGTTTGAAAATAGAACAG+TGG + chr1_1:67339526-67339545 Msa0038090:intron 30.0%
GTGTTTGAAAATAGAACAGT+GGG + chr1_1:67339527-67339546 Msa0038090:intron 30.0%
AATCAAATGTTTCCCATAAG+CGG + chr1_1:67339573-67339592 Msa0038090:intron 30.0%
ATGAAAACAGTTTATGGACA+TGG + chr1_1:67339883-67339902 Msa0038090:intron 30.0%
TTTGCATAAGCAAACTGTTT+GGG - chr1_1:67339931-67339950 None:intergenic 30.0%
ATTTGCATAAGCAAACTGTT+TGG - chr1_1:67339932-67339951 None:intergenic 30.0%
AATCATCTAATCTCCAAAAC+AGG - chr1_1:67340272-67340291 None:intergenic 30.0%
! TTGGAGATTAGATGATTTTG+AGG + chr1_1:67340275-67340294 Msa0038090:three_prime_UTR 30.0%
TTTCATATACCGAGTTTGTA+TGG + chr1_1:67340322-67340341 Msa0038090:three_prime_UTR 30.0%
!!! ATTTTAGGGTTTGTGAGAAT+GGG - chr1_1:67338503-67338522 None:intergenic 30.0%
!!! AATTTTAGGGTTTGTGAGAA+TGG - chr1_1:67338504-67338523 None:intergenic 30.0%
! GAGAATGCGAAAGAAATTTT+AGG - chr1_1:67338518-67338537 None:intergenic 30.0%
!! ATACCATTTTAACCTAACTG+CGG - chr1_1:67338603-67338622 None:intergenic 30.0%
TTGTGATGTGTTATAGTCTT+TGG + chr1_1:67338683-67338702 Msa0038090:intron 30.0%
GTTGGATAATATTATGACAG+TGG + chr1_1:67339036-67339055 Msa0038090:CDS 30.0%
TTTCTGTTCAAAAACCAATC+TGG - chr1_1:67339088-67339107 None:intergenic 30.0%
!! TTGGTTTTTGAACAGAAAGA+AGG + chr1_1:67339090-67339109 Msa0038090:CDS 30.0%
!!! CTCAGTTGGTTTTTTAGTAT+AGG + chr1_1:67339267-67339286 Msa0038090:intron 30.0%
AGTGTTTGAAAATAGAACAG+TGG + chr1_1:67339526-67339545 Msa0038090:intron 30.0%
GTGTTTGAAAATAGAACAGT+GGG + chr1_1:67339527-67339546 Msa0038090:intron 30.0%
AATCAAATGTTTCCCATAAG+CGG + chr1_1:67339573-67339592 Msa0038090:intron 30.0%
ATGAAAACAGTTTATGGACA+TGG + chr1_1:67339883-67339902 Msa0038090:intron 30.0%
TTTGCATAAGCAAACTGTTT+GGG - chr1_1:67339931-67339950 None:intergenic 30.0%
ATTTGCATAAGCAAACTGTT+TGG - chr1_1:67339932-67339951 None:intergenic 30.0%
AATCATCTAATCTCCAAAAC+AGG - chr1_1:67340272-67340291 None:intergenic 30.0%
! TTGGAGATTAGATGATTTTG+AGG + chr1_1:67340275-67340294 Msa0038090:three_prime_UTR 30.0%
TTTCATATACCGAGTTTGTA+TGG + chr1_1:67340322-67340341 Msa0038090:three_prime_UTR 30.0%
TGATGTGTTATAGTCTTTGG+TGG + chr1_1:67338686-67338705 Msa0038090:intron 35.0%
GTGTTGAACACAAATGTTGA+TGG + chr1_1:67338738-67338757 Msa0038090:CDS 35.0%
! TTGTTGGATTTATTTAGGGC+TGG + chr1_1:67338995-67339014 Msa0038090:intron 35.0%
GAACAGAAAGAAGGATTACA+AGG + chr1_1:67339099-67339118 Msa0038090:CDS 35.0%
AGAAAGAAGGATTACAAGGA+TGG + chr1_1:67339103-67339122 Msa0038090:CDS 35.0%
CATGAAGTTGAGAGATGATT+TGG + chr1_1:67339159-67339178 Msa0038090:CDS 35.0%
ACAATGAAGTAGCATCTTAC+CGG - chr1_1:67339201-67339220 None:intergenic 35.0%
AAAGATGATTCTCACTCAGT+TGG + chr1_1:67339253-67339272 Msa0038090:intron 35.0%
AGATGTAGCAGTAAGATGAT+AGG + chr1_1:67339335-67339354 Msa0038090:intron 35.0%
ACTAATAACCATTGCTTCGA+TGG + chr1_1:67339358-67339377 Msa0038090:intron 35.0%
CTGTTATTCAAACACTATCG+AGG - chr1_1:67339406-67339425 None:intergenic 35.0%
ACAGAACGAGAATTCACTAT+TGG + chr1_1:67339422-67339441 Msa0038090:intron 35.0%
TTGCATAAGCAAACTGTTTG+GGG - chr1_1:67339930-67339949 None:intergenic 35.0%
ATGGATGTATGTGGAGAAAT+TGG + chr1_1:67340083-67340102 Msa0038090:intron 35.0%
TGGATGTATGTGGAGAAATT+GGG + chr1_1:67340084-67340103 Msa0038090:intron 35.0%
GTGATTACTGCATAACAGAA+AGG - chr1_1:67340141-67340160 None:intergenic 35.0%
TTCTGTTATGCAGTAATCAC+CGG + chr1_1:67340141-67340160 Msa0038090:intron 35.0%
ATACCACCATTCAACTAATC+AGG - chr1_1:67340403-67340422 None:intergenic 35.0%
TGATGTGTTATAGTCTTTGG+TGG + chr1_1:67338686-67338705 Msa0038090:intron 35.0%
GTGTTGAACACAAATGTTGA+TGG + chr1_1:67338738-67338757 Msa0038090:CDS 35.0%
! TTGTTGGATTTATTTAGGGC+TGG + chr1_1:67338995-67339014 Msa0038090:intron 35.0%
GAACAGAAAGAAGGATTACA+AGG + chr1_1:67339099-67339118 Msa0038090:CDS 35.0%
AGAAAGAAGGATTACAAGGA+TGG + chr1_1:67339103-67339122 Msa0038090:CDS 35.0%
CATGAAGTTGAGAGATGATT+TGG + chr1_1:67339159-67339178 Msa0038090:CDS 35.0%
ACAATGAAGTAGCATCTTAC+CGG - chr1_1:67339201-67339220 None:intergenic 35.0%
AAAGATGATTCTCACTCAGT+TGG + chr1_1:67339253-67339272 Msa0038090:intron 35.0%
AGATGTAGCAGTAAGATGAT+AGG + chr1_1:67339335-67339354 Msa0038090:intron 35.0%
ACTAATAACCATTGCTTCGA+TGG + chr1_1:67339358-67339377 Msa0038090:intron 35.0%
CTGTTATTCAAACACTATCG+AGG - chr1_1:67339406-67339425 None:intergenic 35.0%
ACAGAACGAGAATTCACTAT+TGG + chr1_1:67339422-67339441 Msa0038090:intron 35.0%
TTGCATAAGCAAACTGTTTG+GGG - chr1_1:67339930-67339949 None:intergenic 35.0%
ATGGATGTATGTGGAGAAAT+TGG + chr1_1:67340083-67340102 Msa0038090:intron 35.0%
TGGATGTATGTGGAGAAATT+GGG + chr1_1:67340084-67340103 Msa0038090:intron 35.0%
GTGATTACTGCATAACAGAA+AGG - chr1_1:67340141-67340160 None:intergenic 35.0%
TTCTGTTATGCAGTAATCAC+CGG + chr1_1:67340141-67340160 Msa0038090:intron 35.0%
ATACCACCATTCAACTAATC+AGG - chr1_1:67340403-67340422 None:intergenic 35.0%
! ACGGAGGATTTTGTTGAAGA+GGG - chr1_1:67338580-67338599 None:intergenic 40.0%
!! CATTTTAACCTAACTGCGGA+CGG - chr1_1:67338599-67338618 None:intergenic 40.0%
TTCGCAATGACCTCAATCAA+GGG + chr1_1:67338777-67338796 Msa0038090:CDS 40.0%
! ATGACCTCAATCAAGGGTAT+TGG + chr1_1:67338783-67338802 Msa0038090:CDS 40.0%
CTGATGTTGACATGAACAAG+AGG + chr1_1:67338838-67338857 Msa0038090:CDS 40.0%
TGCAAATCCTCGTCAATTCA+AGG + chr1_1:67339060-67339079 Msa0038090:CDS 40.0%
GTCAATTCAAGGTTCCAGAT+TGG + chr1_1:67339071-67339090 Msa0038090:CDS 40.0%
!! CAACTTCATGTCGAGTTGAT+TGG - chr1_1:67339149-67339168 None:intergenic 40.0%
ATAACCATTGCTTCGATGGT+TGG + chr1_1:67339362-67339381 Msa0038090:intron 40.0%
GTTTCCCATAAGCGGAAATA+CGG + chr1_1:67339581-67339600 Msa0038090:intron 40.0%
TTTCCCATAAGCGGAAATAC+GGG + chr1_1:67339582-67339601 Msa0038090:intron 40.0%
GGAAATACGGGCAATAGAAT+TGG + chr1_1:67339594-67339613 Msa0038090:intron 40.0%
GAATTGGACCAAAAAGTAGC+TGG + chr1_1:67339610-67339629 Msa0038090:intron 40.0%
!!! GATAAGAGCCAGCTACTTTT+TGG - chr1_1:67339621-67339640 None:intergenic 40.0%
AAATAATGGCATGCCCAGAT+AGG - chr1_1:67339973-67339992 None:intergenic 40.0%
TATGTGGAGAAATTGGGCAT+TGG + chr1_1:67340090-67340109 Msa0038090:intron 40.0%
TCTGTTATGCAGTAATCACC+GGG + chr1_1:67340142-67340161 Msa0038090:intron 40.0%
! TTAAGCGTGATGTCCTGTTT+TGG + chr1_1:67340256-67340275 Msa0038090:exon 40.0%
! CAAGTGCCTGATTAGTTGAA+TGG + chr1_1:67340394-67340413 Msa0038090:three_prime_UTR 40.0%
GAGCAAATTGGTCATGCTAA+AGG - chr1_1:67340436-67340455 None:intergenic 40.0%
! ACGGAGGATTTTGTTGAAGA+GGG - chr1_1:67338580-67338599 None:intergenic 40.0%
!! CATTTTAACCTAACTGCGGA+CGG - chr1_1:67338599-67338618 None:intergenic 40.0%
TTCGCAATGACCTCAATCAA+GGG + chr1_1:67338777-67338796 Msa0038090:CDS 40.0%
! ATGACCTCAATCAAGGGTAT+TGG + chr1_1:67338783-67338802 Msa0038090:CDS 40.0%
CTGATGTTGACATGAACAAG+AGG + chr1_1:67338838-67338857 Msa0038090:CDS 40.0%
TGCAAATCCTCGTCAATTCA+AGG + chr1_1:67339060-67339079 Msa0038090:CDS 40.0%
GTCAATTCAAGGTTCCAGAT+TGG + chr1_1:67339071-67339090 Msa0038090:CDS 40.0%
!! CAACTTCATGTCGAGTTGAT+TGG - chr1_1:67339149-67339168 None:intergenic 40.0%
ATAACCATTGCTTCGATGGT+TGG + chr1_1:67339362-67339381 Msa0038090:intron 40.0%
GTTTCCCATAAGCGGAAATA+CGG + chr1_1:67339581-67339600 Msa0038090:intron 40.0%
TTTCCCATAAGCGGAAATAC+GGG + chr1_1:67339582-67339601 Msa0038090:intron 40.0%
GGAAATACGGGCAATAGAAT+TGG + chr1_1:67339594-67339613 Msa0038090:intron 40.0%
GAATTGGACCAAAAAGTAGC+TGG + chr1_1:67339610-67339629 Msa0038090:intron 40.0%
!!! GATAAGAGCCAGCTACTTTT+TGG - chr1_1:67339621-67339640 None:intergenic 40.0%
AAATAATGGCATGCCCAGAT+AGG - chr1_1:67339973-67339992 None:intergenic 40.0%
TATGTGGAGAAATTGGGCAT+TGG + chr1_1:67340090-67340109 Msa0038090:intron 40.0%
TCTGTTATGCAGTAATCACC+GGG + chr1_1:67340142-67340161 Msa0038090:intron 40.0%
! TTAAGCGTGATGTCCTGTTT+TGG + chr1_1:67340256-67340275 Msa0038090:exon 40.0%
! CAAGTGCCTGATTAGTTGAA+TGG + chr1_1:67340394-67340413 Msa0038090:three_prime_UTR 40.0%
GAGCAAATTGGTCATGCTAA+AGG - chr1_1:67340436-67340455 None:intergenic 40.0%
TTAAGGCAGTATGTCGCTCT+GGG - chr1_1:67338546-67338565 None:intergenic 45.0%
! CGGAGGATTTTGTTGAAGAG+GGG - chr1_1:67338579-67338598 None:intergenic 45.0%
! GACGGAGGATTTTGTTGAAG+AGG - chr1_1:67338581-67338600 None:intergenic 45.0%
CGTCCGCAGTTAGGTTAAAA+TGG + chr1_1:67338597-67338616 Msa0038090:exon 45.0%
TGATTGAGGTCATTGCGAAC+TGG - chr1_1:67338776-67338795 None:intergenic 45.0%
GTTCGCAATGACCTCAATCA+AGG + chr1_1:67338776-67338795 Msa0038090:CDS 45.0%
CCTACCAATACCCTTGATTG+AGG - chr1_1:67338790-67338809 None:intergenic 45.0%
!! CCTCAATCAAGGGTATTGGT+AGG + chr1_1:67338787-67338806 Msa0038090:CDS 45.0%
! TGAATTGAGTGCTGCAGAGT+TGG + chr1_1:67339018-67339037 Msa0038090:CDS 45.0%
! TCTGGAACCTTGAATTGACG+AGG - chr1_1:67339070-67339089 None:intergenic 45.0%
CAAGGATGGCAAATTCTCTC+AGG + chr1_1:67339117-67339136 Msa0038090:CDS 45.0%
TGGAGAGACTCAAGAAGATC+CGG + chr1_1:67339179-67339198 Msa0038090:CDS 45.0%
ACACGATCAAAGCAATGCAG+TGG - chr1_1:67339484-67339503 None:intergenic 45.0%
TTGCCCGTATTTCCGCTTAT+GGG - chr1_1:67339588-67339607 None:intergenic 45.0%
ATTGCCCGTATTTCCGCTTA+TGG - chr1_1:67339589-67339608 None:intergenic 45.0%
CTGTTATGCAGTAATCACCG+GGG + chr1_1:67340143-67340162 Msa0038090:intron 45.0%
! GTGCCTGATTAGTTGAATGG+TGG + chr1_1:67340397-67340416 Msa0038090:three_prime_UTR 45.0%
TTAAGGCAGTATGTCGCTCT+GGG - chr1_1:67338546-67338565 None:intergenic 45.0%
! CGGAGGATTTTGTTGAAGAG+GGG - chr1_1:67338579-67338598 None:intergenic 45.0%
! GACGGAGGATTTTGTTGAAG+AGG - chr1_1:67338581-67338600 None:intergenic 45.0%
CGTCCGCAGTTAGGTTAAAA+TGG + chr1_1:67338597-67338616 Msa0038090:exon 45.0%
TGATTGAGGTCATTGCGAAC+TGG - chr1_1:67338776-67338795 None:intergenic 45.0%
GTTCGCAATGACCTCAATCA+AGG + chr1_1:67338776-67338795 Msa0038090:CDS 45.0%
CCTACCAATACCCTTGATTG+AGG - chr1_1:67338790-67338809 None:intergenic 45.0%
!! CCTCAATCAAGGGTATTGGT+AGG + chr1_1:67338787-67338806 Msa0038090:CDS 45.0%
! TGAATTGAGTGCTGCAGAGT+TGG + chr1_1:67339018-67339037 Msa0038090:CDS 45.0%
! TCTGGAACCTTGAATTGACG+AGG - chr1_1:67339070-67339089 None:intergenic 45.0%
CAAGGATGGCAAATTCTCTC+AGG + chr1_1:67339117-67339136 Msa0038090:CDS 45.0%
TGGAGAGACTCAAGAAGATC+CGG + chr1_1:67339179-67339198 Msa0038090:CDS 45.0%
ACACGATCAAAGCAATGCAG+TGG - chr1_1:67339484-67339503 None:intergenic 45.0%
TTGCCCGTATTTCCGCTTAT+GGG - chr1_1:67339588-67339607 None:intergenic 45.0%
ATTGCCCGTATTTCCGCTTA+TGG - chr1_1:67339589-67339608 None:intergenic 45.0%
CTGTTATGCAGTAATCACCG+GGG + chr1_1:67340143-67340162 Msa0038090:intron 45.0%
! GTGCCTGATTAGTTGAATGG+TGG + chr1_1:67340397-67340416 Msa0038090:three_prime_UTR 45.0%
CTTAAGGCAGTATGTCGCTC+TGG - chr1_1:67338547-67338566 None:intergenic 50.0%
GAGCGACATACTGCCTTAAG+CGG + chr1_1:67338547-67338566 Msa0038090:five_prime_UTR 50.0%
! AGAGGGGTAATTGCCGCTTA+AGG - chr1_1:67338563-67338582 None:intergenic 50.0%
AAAATCCTCCGTCCGCAGTT+AGG + chr1_1:67338588-67338607 Msa0038090:five_prime_UTR 50.0%
TTTAACCTAACTGCGGACGG+AGG - chr1_1:67338596-67338615 None:intergenic 50.0%
AGCACCAACCATCGAAGCAA+TGG - chr1_1:67339369-67339388 None:intergenic 50.0%
CGATCAAAGCAATGCAGTGG+TGG - chr1_1:67339481-67339500 None:intergenic 50.0%
!! TGGAGAAATTGGGCATTGGC+TGG + chr1_1:67340094-67340113 Msa0038090:intron 50.0%
TATGCTGACCACGAACACGA+AGG - chr1_1:67340187-67340206 None:intergenic 50.0%
GGTCAGCATACCAAGACTAC+AGG + chr1_1:67340197-67340216 Msa0038090:CDS 50.0%
CTTAAGGCAGTATGTCGCTC+TGG - chr1_1:67338547-67338566 None:intergenic 50.0%
GAGCGACATACTGCCTTAAG+CGG + chr1_1:67338547-67338566 Msa0038090:five_prime_UTR 50.0%
! AGAGGGGTAATTGCCGCTTA+AGG - chr1_1:67338563-67338582 None:intergenic 50.0%
AAAATCCTCCGTCCGCAGTT+AGG + chr1_1:67338588-67338607 Msa0038090:five_prime_UTR 50.0%
TTTAACCTAACTGCGGACGG+AGG - chr1_1:67338596-67338615 None:intergenic 50.0%
AGCACCAACCATCGAAGCAA+TGG - chr1_1:67339369-67339388 None:intergenic 50.0%
CGATCAAAGCAATGCAGTGG+TGG - chr1_1:67339481-67339500 None:intergenic 50.0%
!! TGGAGAAATTGGGCATTGGC+TGG + chr1_1:67340094-67340113 Msa0038090:intron 50.0%
TATGCTGACCACGAACACGA+AGG - chr1_1:67340187-67340206 None:intergenic 50.0%
GGTCAGCATACCAAGACTAC+AGG + chr1_1:67340197-67340216 Msa0038090:CDS 50.0%
! ATGCAGTGGTGGTGCATCAG+CGG - chr1_1:67339470-67339489 None:intergenic 55.0%
GCAGTAATCACCGGGGTTTG+AGG + chr1_1:67340150-67340169 Msa0038090:intron 55.0%
ATACCAAGACTACAGGCCGC+AGG + chr1_1:67340204-67340223 Msa0038090:CDS 55.0%
TACCAAGACTACAGGCCGCA+GGG + chr1_1:67340205-67340224 Msa0038090:CDS 55.0%
CACCAACAGTCTTTCCCCTG+CGG - chr1_1:67340223-67340242 None:intergenic 55.0%
! ATGCAGTGGTGGTGCATCAG+CGG - chr1_1:67339470-67339489 None:intergenic 55.0%
GCAGTAATCACCGGGGTTTG+AGG + chr1_1:67340150-67340169 Msa0038090:intron 55.0%
ATACCAAGACTACAGGCCGC+AGG + chr1_1:67340204-67340223 Msa0038090:CDS 55.0%
TACCAAGACTACAGGCCGCA+GGG + chr1_1:67340205-67340224 Msa0038090:CDS 55.0%
CACCAACAGTCTTTCCCCTG+CGG - chr1_1:67340223-67340242 None:intergenic 55.0%
! ACCGGGGTTTGAGGCACTAC+TGG + chr1_1:67340159-67340178 Msa0038090:CDS 60.0%
CCCAGTAGTGCCTCAAACCC+CGG - chr1_1:67340163-67340182 None:intergenic 60.0%
! CCGGGGTTTGAGGCACTACT+GGG + chr1_1:67340160-67340179 Msa0038090:CDS 60.0%
! CGGGGTTTGAGGCACTACTG+GGG + chr1_1:67340161-67340180 Msa0038090:CDS 60.0%
TACTGGGGCCTTCGTGTTCG+TGG + chr1_1:67340176-67340195 Msa0038090:CDS 60.0%
ACCAAGACTACAGGCCGCAG+GGG + chr1_1:67340206-67340225 Msa0038090:CDS 60.0%
! ACCGGGGTTTGAGGCACTAC+TGG + chr1_1:67340159-67340178 Msa0038090:CDS 60.0%
CCCAGTAGTGCCTCAAACCC+CGG - chr1_1:67340163-67340182 None:intergenic 60.0%
! CCGGGGTTTGAGGCACTACT+GGG + chr1_1:67340160-67340179 Msa0038090:CDS 60.0%
! CGGGGTTTGAGGCACTACTG+GGG + chr1_1:67340161-67340180 Msa0038090:CDS 60.0%
TACTGGGGCCTTCGTGTTCG+TGG + chr1_1:67340176-67340195 Msa0038090:CDS 60.0%
ACCAAGACTACAGGCCGCAG+GGG + chr1_1:67340206-67340225 Msa0038090:CDS 60.0%
TCCCCTGCGGCCTGTAGTCT+TGG - chr1_1:67340210-67340229 None:intergenic 65.0%
GGCCGCAGGGGAAAGACTGT+TGG + chr1_1:67340218-67340237 Msa0038090:CDS 65.0%
TCCCCTGCGGCCTGTAGTCT+TGG - chr1_1:67340210-67340229 None:intergenic 65.0%
GGCCGCAGGGGAAAGACTGT+TGG + chr1_1:67340218-67340237 Msa0038090:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 67338499 67340513 67338499 ID=Msa0038090;Name=Msa0038090
chr1_1 mRNA 67338499 67340513 67338499 ID=Msa0038090-mRNA-1;Parent=Msa0038090;Name=Msa0038090-mRNA-1;_AED=0.06;_eAED=0.06;_QI=117|1|1|1|0.75|0.6|5|222|152
chr1_1 exon 67338499 67338618 67338499 ID=Msa0038090-mRNA-1:exon:21883;Parent=Msa0038090-mRNA-1
chr1_1 exon 67338699 67338859 67338699 ID=Msa0038090-mRNA-1:exon:21884;Parent=Msa0038090-mRNA-1
chr1_1 exon 67339012 67339200 67339012 ID=Msa0038090-mRNA-1:exon:21885;Parent=Msa0038090-mRNA-1
chr1_1 exon 67340154 67340428 67340154 ID=Msa0038090-mRNA-1:exon:21886;Parent=Msa0038090-mRNA-1
chr1_1 exon 67340461 67340513 67340461 ID=Msa0038090-mRNA-1:exon:21887;Parent=Msa0038090-mRNA-1
chr1_1 five_prime_UTR 67338499 67338615 67338499 ID=Msa0038090-mRNA-1:five_prime_utr;Parent=Msa0038090-mRNA-1
chr1_1 CDS 67338616 67338618 67338616 ID=Msa0038090-mRNA-1:cds;Parent=Msa0038090-mRNA-1
chr1_1 CDS 67338699 67338859 67338699 ID=Msa0038090-mRNA-1:cds;Parent=Msa0038090-mRNA-1
chr1_1 CDS 67339012 67339200 67339012 ID=Msa0038090-mRNA-1:cds;Parent=Msa0038090-mRNA-1
chr1_1 CDS 67340154 67340259 67340154 ID=Msa0038090-mRNA-1:cds;Parent=Msa0038090-mRNA-1
chr1_1 three_prime_UTR 67340260 67340428 67340260 ID=Msa0038090-mRNA-1:three_prime_utr;Parent=Msa0038090-mRNA-1
chr1_1 three_prime_UTR 67340461 67340513 67340461 ID=Msa0038090-mRNA-1:three_prime_utr;Parent=Msa0038090-mRNA-1
Gene Sequence

>Msa0038090

ATGTCTTTGGTGGCGAACGAAGAATTTCAGCACATTCTGCGTGTGTTGAACACAAATGTTGATGGAAAGCAGAAGATCCAGTTCGCAATGACCTCAATCAAGGGTATTGGTAGGAGATTCGCTAACATCTGCTGTAAGAAAGCTGATGTTGACATGAACAAGAGGGCTGGTGAATTGAGTGCTGCAGAGTTGGATAATATTATGACAGTGGTTGCAAATCCTCGTCAATTCAAGGTTCCAGATTGGTTTTTGAACAGAAAGAAGGATTACAAGGATGGCAAATTCTCTCAGGTTGTCTCCAATCAACTCGACATGAAGTTGAGAGATGATTTGGAGAGACTCAAGAAGATCCGTAATCACCGGGGTTTGAGGCACTACTGGGGCCTTCGTGTTCGTGGTCAGCATACCAAGACTACAGGCCGCAGGGGAAAGACTGTTGGTGTCTCAAAGAAGCGTTAA
Protein sequence

>Msa0038090

MSLVANEEFQHILRVLNTNVDGKQKIQFAMTSIKGIGRRFANICCKKADVDMNKRAGELSAAELDNIMTVVANPRQFKVPDWFLNRKKDYKDGKFSQVVSNQLDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQHTKTTGRRGKTVGVSKKR*