AlfalfaGEDB | Msa0576730

M. sativa cultivar zhongmu-4 / Msa0576730

KEGG: K10865
GO: GO:0000228 GO:0000785 GO:0000791 GO:0000793 GO:0000794 GO:0000795 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0099086
PFAM: PF03094.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576730	RHN75196.1	88.235	68	8	0	34	101	498	565	1.63e-28	119

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576730	A0A396JA88	88.235	68	8	0	34	101	498	565	7.79e-29	119

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008090	Msa0576730	0.803005	4.285548e-49	-8.615850e-47
Msa0008840	Msa0576730	0.800785	1.224491e-48	-8.615850e-47
Msa0020390	Msa0576730	0.805185	1.508460e-49	-8.615850e-47
Msa0024710	Msa0576730	0.806708	7.213167e-50	-8.615850e-47
Msa0026660	Msa0576730	0.804325	2.280843e-49	-8.615850e-47
Msa0027740	Msa0576730	0.853040	3.107086e-61	-8.615850e-47
Msa0033880	Msa0576730	0.810526	1.103326e-50	-8.615850e-47
Msa0049390	Msa0576730	0.826463	2.675718e-54	-8.615850e-47
Msa0053690	Msa0576730	0.802981	4.335057e-49	-8.615850e-47
Msa0053700	Msa0576730	0.816488	5.390547e-52	-8.615850e-47
Msa0055100	Msa0576730	0.813816	2.112926e-51	-8.615850e-47
Msa0062120	Msa0576730	0.803810	2.919053e-49	-8.615850e-47
Msa0065010	Msa0576730	0.804361	2.241656e-49	-8.615850e-47
Msa0068020	Msa0576730	0.805016	1.635950e-49	-8.615850e-47
Msa0070170	Msa0576730	0.800326	1.518667e-48	-8.615850e-47
Msa0097590	Msa0576730	0.808997	2.352275e-50	-8.615850e-47
Msa0097610	Msa0576730	0.811095	8.307428e-51	-8.615850e-47
Msa0101180	Msa0576730	0.830550	2.756439e-55	-8.615850e-47
Msa0115370	Msa0576730	0.803316	3.694079e-49	-8.615850e-47
Msa0118900	Msa0576730	0.805906	1.064599e-49	-8.615850e-47
Msa0126770	Msa0576730	0.822843	1.906404e-53	-8.615850e-47
Msa0126840	Msa0576730	0.806680	7.313260e-50	-8.615850e-47
Msa0126880	Msa0576730	0.806136	9.524259e-50	-8.615850e-47
Msa0127850	Msa0576730	0.803970	2.703994e-49	-8.615850e-47
Msa0164370	Msa0576730	0.837677	4.512874e-57	-8.615850e-47
Msa0167980	Msa0576730	0.824249	8.940793e-54	-8.615850e-47
Msa0168190	Msa0576730	0.872816	2.377467e-67	-8.615850e-47
Msa0171850	Msa0576730	0.802231	6.189429e-49	-8.615850e-47
Msa0174000	Msa0576730	0.821731	3.454168e-53	-8.615850e-47
Msa0175200	Msa0576730	0.838400	2.940555e-57	-8.615850e-47
Msa0183870	Msa0576730	0.823685	1.212234e-53	-8.615850e-47
Msa0193600	Msa0576730	0.800550	1.367483e-48	-8.615850e-47
Msa0207360	Msa0576730	0.802948	4.403088e-49	-8.615850e-47
Msa0207410	Msa0576730	0.824895	6.298215e-54	-8.615850e-47
Msa0207440	Msa0576730	0.815284	1.000159e-51	-8.615850e-47
Msa0364990	Msa0576730	0.846113	2.663952e-59	-8.615850e-47
Msa0379570	Msa0576730	0.808161	3.547816e-50	-8.615850e-47
Msa0382370	Msa0576730	0.802547	5.327551e-49	-8.615850e-47
Msa0384670	Msa0576730	0.817193	3.744733e-52	-8.615850e-47
Msa0394280	Msa0576730	0.843688	1.201074e-58	-8.615850e-47
Msa0414020	Msa0576730	0.835175	1.954899e-56	-8.615850e-47
Msa0450480	Msa0576730	0.810916	9.083707e-51	-8.615850e-47
Msa0458640	Msa0576730	0.837055	6.515005e-57	-8.615850e-47
Msa0482180	Msa0576730	0.808407	3.144889e-50	-8.615850e-47
Msa0257490	Msa0576730	0.808939	2.420517e-50	-8.615850e-47
Msa0296790	Msa0576730	0.821299	4.347846e-53	-8.615850e-47
Msa0296920	Msa0576730	0.802772	4.787879e-49	-8.615850e-47
Msa0304660	Msa0576730	0.809203	2.125190e-50	-8.615850e-47
Msa0331700	Msa0576730	0.808163	3.544529e-50	-8.615850e-47
Msa0337510	Msa0576730	0.833091	6.508480e-56	-8.615850e-47
Msa0511770	Msa0576730	0.812120	4.975166e-51	-8.615850e-47
Msa0527910	Msa0576730	0.816747	4.715748e-52	-8.615850e-47
Msa0530100	Msa0576730	0.817336	3.477437e-52	-8.615850e-47
Msa0531250	Msa0576730	0.807642	4.574366e-50	-8.615850e-47
Msa0539050	Msa0576730	0.832738	7.966005e-56	-8.615850e-47
Msa0539060	Msa0576730	0.830735	2.483851e-55	-8.615850e-47
Msa0550540	Msa0576730	0.802981	4.334641e-49	-8.615850e-47
Msa0550570	Msa0576730	0.800952	1.132076e-48	-8.615850e-47
Msa0576730	Msa0594710	0.803471	3.432116e-49	-8.615850e-47
Msa0576730	Msa0603670	0.805085	1.582272e-49	-8.615850e-47
Msa0576730	Msa0609000	0.802408	5.691305e-49	-8.615850e-47
Msa0576730	Msa0626940	0.803650	3.150995e-49	-8.615850e-47
Msa0576730	Msa0628420	0.813852	2.075458e-51	-8.615850e-47
Msa0576730	Msa0662630	0.807266	5.496821e-50	-8.615850e-47
Msa0576730	Msa0682140	0.802685	4.988276e-49	-8.615850e-47
Msa0576730	Msa0687230	0.813010	3.178209e-51	-8.615850e-47
Msa0576730	Msa0689500	0.800681	1.285910e-48	-8.615850e-47
Msa0576730	Msa0699190	0.826303	2.921686e-54	-8.615850e-47
Msa0576730	Msa0702150	0.960746	6.608956e-119	-8.615850e-47
Msa0576730	Msa0702270	0.985537	7.117310e-164	-8.615850e-47
Msa0576730	Msa0703910	0.806835	6.783411e-50	-8.615850e-47
Msa0576730	Msa0705390	0.815241	1.022571e-51	-8.615850e-47
Msa0576730	Msa0748920	0.822957	1.793500e-53	-8.615850e-47
Msa0576730	Msa0751010	0.804149	2.481283e-49	-8.615850e-47
Msa0576730	Msa0758230	0.801803	7.578105e-49	-8.615850e-47
Msa0576730	Msa0764690	0.809263	2.062566e-50	-8.615850e-47
Msa0576730	Msa0792780	0.828228	1.010483e-54	-8.615850e-47
Msa0576730	Msa0794050	0.820974	5.165992e-53	-8.615850e-47
Msa0576730	Msa0799480	0.803554	3.298501e-49	-8.615850e-47
Msa0576730	Msa0895310	0.820529	6.536719e-53	-8.615850e-47
Msa0576730	Msa0901110	0.818988	1.470740e-52	-8.615850e-47
Msa0576730	Msa0903780	0.803954	2.724117e-49	-8.615850e-47
Msa0576730	Msa0943270	0.807422	5.094658e-50	-8.615850e-47
Msa0576730	Msa0944200	0.806287	8.851176e-50	-8.615850e-47
Msa0576730	Msa0990830	0.810920	9.067062e-51	-8.615850e-47
Msa0576730	Msa1012540	0.813503	2.476604e-51	-8.615850e-47
Msa0576730	Msa1020100	0.801834	7.469552e-49	-8.615850e-47
Msa0576730	Msa1025260	0.818762	1.655005e-52	-8.615850e-47
Msa0576730	Msa1027250	0.806425	8.278115e-50	-8.615850e-47
Msa0576730	Msa1059310	0.809218	2.109582e-50	-8.615850e-47
Msa0576730	Msa1067070	0.829021	6.499501e-55	-8.615850e-47
Msa0576730	Msa1083880	0.810171	1.315905e-50	-8.615850e-47
Msa0576730	Msa1109350	0.813395	2.615685e-51	-8.615850e-47
Msa0576730	Msa1126850	0.812320	4.497694e-51	-8.615850e-47
Msa0576730	Msa1154490	0.805044	1.614263e-49	-8.615850e-47
Msa0576730	Msa1173900	0.832541	8.913746e-56	-8.615850e-47
Msa0576730	Msa1176360	0.816249	6.093939e-52	-8.615850e-47
Msa0576730	Msa1202580	0.816822	4.536867e-52	-8.615850e-47
Msa0576730	Msa1205850	0.801392	9.201884e-49	-8.615850e-47
Msa0576730	Msa1206310	0.815974	7.020159e-52	-8.615850e-47
Msa0576730	Msa1206360	0.804318	2.288074e-49	-8.615850e-47
Msa0576730	Msa1211830	0.848949	4.423368e-60	-8.615850e-47
Msa0576730	Msa1253400	0.813810	2.120388e-51	-8.615850e-47
Msa0576730	Msa1253810	0.844048	9.619967e-59	-8.615850e-47
Msa0576730	Msa1258370	0.810502	1.116536e-50	-8.615850e-47
Msa0576730	Msa1259640	0.804332	2.273277e-49	-8.615850e-47
Msa0576730	Msa1265590	0.830923	2.234377e-55	-8.615850e-47
Msa0576730	Msa1278900	0.827132	1.852008e-54	-8.615850e-47
Msa0576730	Msa1279590	0.828798	7.356909e-55	-8.615850e-47
Msa0576730	Msa1289300	0.835012	2.149130e-56	-8.615850e-47
Msa0576730	Msa1297600	0.836823	7.465264e-57	-8.615850e-47
Msa0576730	Msa1299700	0.823449	1.377176e-53	-8.615850e-47
Msa0576730	Msa1303580	0.809493	1.841286e-50	-8.615850e-47
Msa0576730	Msa1307180	0.805414	1.350725e-49	-8.615850e-47
Msa0576730	Msa1316360	0.802161	6.395947e-49	-8.615850e-47
Msa0576730	Msa1320580	0.809254	2.071770e-50	-8.615850e-47
Msa0576730	Msa1333970	0.807727	4.389402e-50	-8.615850e-47
Msa0576730	Msa1351540	0.808333	3.261370e-50	-8.615850e-47
Msa0576730	Msa1372310	0.816455	5.483078e-52	-8.615850e-47
Msa0576730	Msa1381480	0.812187	4.810558e-51	-8.615850e-47
Msa0576730	Msa1381650	0.814587	1.428259e-51	-8.615850e-47
Msa0576730	Msa1411950	0.820865	5.472992e-53	-8.615850e-47
Msa0576730	Msa1416370	0.802608	5.175621e-49	-8.615850e-47
Msa0576730	Msa1433600	0.812716	3.686416e-51	-8.615850e-47
Msa0576730	Msa1434340	0.804399	2.200944e-49	-8.615850e-47
Msa0576730	Msa1437270	0.815210	1.038844e-51	-8.615850e-47
Msa0576730	Msa1445940	0.820089	8.245463e-53	-8.615850e-47
Msa0576730	Msa1452570	0.817539	3.129302e-52	-8.615850e-47
Msa0576730	Msa1455280	0.841139	5.694969e-58	-8.615850e-47
Msa0576730	Msa1457220	0.812100	5.023386e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576730	MtrunA17_Chr2g0318541	88.235	68	8	0	34	101	498	565	1.50e-32	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 131 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTAGTTACTATCATATTT+AGG	0.140806	4_1:+80795965	Msa0576730:three_prime_UTR
CCTGAATGACATTGATATTC+TGG	0.189504	4_1:-80795483	None:intergenic
AAGAACAGTATACATGTAAA+TGG	0.205118	4_1:-80796214	None:intergenic
TGGGCACTTTAGGGGCATTT+TGG	0.228149	4_1:+80792274	Msa0576730:five_prime_UTR
ATTAGTTACTATCATATTTA+GGG	0.265584	4_1:+80795966	Msa0576730:three_prime_UTR
TTCTAAAGACTGGTATGTTT+TGG	0.266573	4_1:-80792436	None:intergenic
TGCACTTTGTTGAATTGTTT+TGG	0.271540	4_1:+80796011	Msa0576730:three_prime_UTR
TCCAATTGATGAAATTTCTT+TGG	0.273796	4_1:+80792302	Msa0576730:five_prime_UTR
TTACTAGCTTAAGACATAAA+TGG	0.278661	4_1:+80795013	Msa0576730:three_prime_UTR
ATTAAGGCTTGGTTTCATTA+AGG	0.279842	4_1:+80793621	Msa0576730:CDS
CAAATTCGATTTACTGTTCA+TGG	0.283346	4_1:-80792462	None:intergenic
GAAGAACAATACTTTGCTTT+TGG	0.289019	4_1:+80795817	Msa0576730:three_prime_UTR
GTCATGTAGTGGTGCAGTTT+TGG	0.298095	4_1:+80792651	Msa0576730:five_prime_UTR
ACCAAAGAAATTTCATCAAT+TGG	0.303256	4_1:-80792303	None:intergenic
CCAACGTTCATTGATTATAA+TGG	0.304555	4_1:+80795688	Msa0576730:three_prime_UTR
GTGACACCTCCACTAACATT+TGG	0.310417	4_1:+80792324	Msa0576730:five_prime_UTR
CCAGAATATCAATGTCATTC+AGG	0.310628	4_1:+80795483	Msa0576730:three_prime_UTR
CTGAGGATACCGTGTTCTTC+CGG	0.313280	4_1:+80794848	Msa0576730:three_prime_UTR
AGTTGAACTATGCGACATTA+AGG	0.318921	4_1:+80793605	Msa0576730:CDS
TAACATTTGGGCTATATATA+TGG	0.319105	4_1:+80792337	Msa0576730:five_prime_UTR
TCTAATTCTTGTTGTTACTA+AGG	0.369203	4_1:+80794729	Msa0576730:three_prime_UTR
TTCTGAGATTGGTTTGAGTT+GGG	0.378227	4_1:+80792586	Msa0576730:five_prime_UTR
ATGCGATAGGGTTCTGAGAT+TGG	0.378475	4_1:+80792575	Msa0576730:five_prime_UTR
TGAAATTTAAGTGTAGAATC+AGG	0.382944	4_1:+80796152	Msa0576730:three_prime_UTR
CCTTTCCGACCTCTGGTGCT+TGG	0.384030	4_1:-80794229	None:intergenic
GTTCTTTGATCTGCTGTTGT+TGG	0.385533	4_1:+80792608	Msa0576730:five_prime_UTR
TCAATGAACGTTGGTGTAGC+TGG	0.388479	4_1:-80795679	None:intergenic
TGAGGATACCGTGTTCTTCC+GGG	0.388485	4_1:+80794849	Msa0576730:three_prime_UTR
CTGATACATGTATCTCCTAT+TGG	0.389711	4_1:-80795900	None:intergenic
GTATCTGAATCTACTCCTTA+TGG	0.395535	4_1:+80795606	Msa0576730:three_prime_UTR
TCCTGTTTGTTATAGCCATA+AGG	0.397282	4_1:-80795621	None:intergenic
TCTTGGTGCTCAGTCCTTTG+AGG	0.398508	4_1:+80793989	Msa0576730:CDS
ACAAAATAGAGCTGGGTGCC+TGG	0.399485	4_1:+80795566	Msa0576730:three_prime_UTR
AAGTCTCTGGTATGTAAAAT+TGG	0.405635	4_1:+80794655	Msa0576730:three_prime_UTR
TCTGTGAATAACCATATGAT+TGG	0.408436	4_1:+80794820	Msa0576730:three_prime_UTR
TGAAAATGTGATCACAATCA+TGG	0.410902	4_1:+80793522	Msa0576730:exon
GTTCTGAGATTGGTTTGAGT+TGG	0.411370	4_1:+80792585	Msa0576730:five_prime_UTR
CTGCACTCTCTGAACTAATC+AGG	0.411911	4_1:+80796117	Msa0576730:three_prime_UTR
TGTGTCATGGTTTATTGTAA+TGG	0.412851	4_1:+80796074	Msa0576730:three_prime_UTR
TCTCCTATTGGCACCGTCAA+TGG	0.417525	4_1:-80795888	None:intergenic
AACAGATCCTTTCCGACCTC+TGG	0.421775	4_1:-80794236	None:intergenic
ATGACATTGATATTCTGGTT+TGG	0.423705	4_1:-80795478	None:intergenic
TGACACCTCCACTAACATTT+GGG	0.425550	4_1:+80792325	Msa0576730:five_prime_UTR
TATTTAGGGTCATGGTGTGC+AGG	0.430442	4_1:+80795980	Msa0576730:three_prime_UTR
ACGCAGCTCCTGCCATGGTC+AGG	0.431291	4_1:+80794532	Msa0576730:intron
TGGCTATAACAAACAGGAAA+GGG	0.435466	4_1:+80795626	Msa0576730:three_prime_UTR
AGTGAATTGTGAAGATTCCT+TGG	0.446636	4_1:-80793967	None:intergenic
TAATATATCCTGCTCTTCAC+TGG	0.447204	4_1:-80795947	None:intergenic
CCTCAGCTTGCCCAATCATA+TGG	0.450278	4_1:-80794831	None:intergenic
TGATGATTATTACACTTAAG+TGG	0.450769	4_1:-80795851	None:intergenic
AGGGTCATGGTGTGCAGGGT+TGG	0.456110	4_1:+80795985	Msa0576730:three_prime_UTR
TCATTTCCTTCTTCAGATTG+TGG	0.458373	4_1:+80792365	Msa0576730:five_prime_UTR
TCATCATCGCTGAAGGAAAC+CGG	0.458878	4_1:-80794178	None:intergenic
CACAGAAGCCTGACCATGGC+AGG	0.462409	4_1:-80794540	None:intergenic
ATCACTTTCATTGATTATTG+TGG	0.462962	4_1:+80794592	Msa0576730:three_prime_UTR
CAATAGGAGATACATGTATC+AGG	0.463833	4_1:+80795901	Msa0576730:three_prime_UTR
CGCATATGTCAGGCAAATCC+AGG	0.467174	4_1:-80795584	None:intergenic
TTCACAGAACAAGGCTAGAA+TGG	0.468394	4_1:+80795396	Msa0576730:intron
GAATCTTCACAATTCACTCT+TGG	0.470201	4_1:+80793972	Msa0576730:CDS
TCCTTATGGCTATAACAAAC+AGG	0.471089	4_1:+80795620	Msa0576730:three_prime_UTR
TACTATCATATTTAGGGTCA+TGG	0.476367	4_1:+80795972	Msa0576730:three_prime_UTR
TTCATGGGGTTTCTAAAGAC+TGG	0.477011	4_1:-80792446	None:intergenic
ATTTATCTGTGATTTGTTCG+TGG	0.478617	4_1:+80794769	Msa0576730:three_prime_UTR
AGTCCATTGACGGTGCCAAT+AGG	0.481728	4_1:+80795885	Msa0576730:three_prime_UTR
TTCGTTGTCCAGTGAAGAGC+AGG	0.484634	4_1:+80795939	Msa0576730:three_prime_UTR
TGTGCAAGTACGCAAGTCTC+TGG	0.485815	4_1:+80794642	Msa0576730:three_prime_UTR
TGCAGCTGGAGCTGATAGTC+CGG	0.486818	4_1:+80794159	Msa0576730:CDS
CCCCTAAAGTGCCCAAAATG+AGG	0.487598	4_1:-80792266	None:intergenic
GTGTCAGCATGCATTTCCCT+TGG	0.489277	4_1:+80795727	Msa0576730:three_prime_UTR
CTGTGAATAACCATATGATT+GGG	0.489653	4_1:+80794821	Msa0576730:three_prime_UTR
TTTGTTCTAGGAACGTGTTA+AGG	0.492658	4_1:+80793929	Msa0576730:intron
AGAGCAAGACAAAACCTTCC+CGG	0.495380	4_1:-80794867	None:intergenic
AACTATGCGACATTAAGGCT+TGG	0.498825	4_1:+80793610	Msa0576730:CDS
TGCAGCAATAATTCAAAGAC+AGG	0.499476	4_1:-80796099	None:intergenic
AGAACAGTATACATGTAAAT+GGG	0.499738	4_1:-80796213	None:intergenic
AGAGTTCTACAAAATAGAGC+TGG	0.500119	4_1:+80795558	Msa0576730:three_prime_UTR
ATATATAGCCCAAATGTTAG+TGG	0.502458	4_1:-80792333	None:intergenic
TTTCAAGGCAGAAGTGCAGC+TGG	0.505124	4_1:+80794145	Msa0576730:CDS
CGGTTTGCACCAGAACTTGG+TGG	0.505268	4_1:+80794963	Msa0576730:intron
GATTCAGATACGCATATGTC+AGG	0.506496	4_1:-80795594	None:intergenic
ATTTAGGGTCATGGTGTGCA+GGG	0.511669	4_1:+80795981	Msa0576730:three_prime_UTR
AAATTCGATTTACTGTTCAT+GGG	0.512240	4_1:-80792461	None:intergenic
TCTTGTTGTTACTAAGGAGA+GGG	0.512640	4_1:+80794735	Msa0576730:three_prime_UTR
ATTTGTTGGGTTTACCTCAA+AGG	0.513130	4_1:-80794003	None:intergenic
ACATTCTTTGACAAAACAGT+TGG	0.514565	4_1:-80795419	None:intergenic
GAAAATGTGATCACAATCAT+GGG	0.515424	4_1:+80793523	Msa0576730:exon
TGGAATGGAATTTGTTGGGT+AGG	0.516364	4_1:-80795659	None:intergenic
TGTAAAATTGGAGGTGAGTG+GGG	0.520147	4_1:+80794667	Msa0576730:three_prime_UTR
TTTATGTCTTAAGCTAGTAA+AGG	0.520978	4_1:-80795010	None:intergenic
ATGTAAAATTGGAGGTGAGT+GGG	0.523440	4_1:+80794666	Msa0576730:three_prime_UTR
TAGCTGGAATGGAATTTGTT+GGG	0.523834	4_1:-80795663	None:intergenic
GTAGCTGGAATGGAATTTGT+TGG	0.537737	4_1:-80795664	None:intergenic
CTGGACAACGAATGAGATGC+AGG	0.540409	4_1:-80795928	None:intergenic
GAACGTTGGTGTAGCTGGAA+TGG	0.542196	4_1:-80795674	None:intergenic
ATGAAACCACAATCTGAAGA+AGG	0.543903	4_1:-80792371	None:intergenic
AGTTAGCATACTATCTTCCA+AGG	0.544019	4_1:-80795744	None:intergenic
CTCACATCTCCACTCGCATC+CGG	0.551685	4_1:-80795778	None:intergenic
ACATGTTCACTGTTGTGTCA+TGG	0.552124	4_1:+80796061	Msa0576730:three_prime_UTR
GTGTTTGTGAAGTCCATTGA+CGG	0.552423	4_1:+80795875	Msa0576730:three_prime_UTR
CGATGAACAGTAATGCGATA+GGG	0.553516	4_1:+80792563	Msa0576730:five_prime_UTR
GGAGCGATCTACGCGCTCCA+AGG	0.554334	4_1:+80793950	Msa0576730:CDS
ATGGCTATAACAAACAGGAA+AGG	0.562348	4_1:+80795625	Msa0576730:three_prime_UTR
TTCTTGTTGTTACTAAGGAG+AGG	0.564790	4_1:+80794734	Msa0576730:three_prime_UTR
TTTCAACGCAGCTCCTGCCA+TGG	0.567402	4_1:+80794527	Msa0576730:intron
GGCATCTTCATCATCGCTGA+AGG	0.569407	4_1:-80794185	None:intergenic
TCGATGAACAGTAATGCGAT+AGG	0.572164	4_1:+80792562	Msa0576730:five_prime_UTR
CCCTAAAGTGCCCAAAATGA+GGG	0.576444	4_1:-80792265	None:intergenic
TTATAATGGTGAAACTAGCT+TGG	0.578050	4_1:+80795702	Msa0576730:three_prime_UTR
CCAAGCACCAGAGGTCGGAA+AGG	0.579149	4_1:+80794229	Msa0576730:CDS
TCTCTGGTATGTAAAATTGG+AGG	0.581652	4_1:+80794658	Msa0576730:three_prime_UTR
AATTCGATTTACTGTTCATG+GGG	0.602667	4_1:-80792460	None:intergenic
TGCTAACTTGTTGATCATGC+CGG	0.603742	4_1:+80795759	Msa0576730:three_prime_UTR
CCATTATAATCAATGAACGT+TGG	0.604275	4_1:-80795688	None:intergenic
GAGTTCTACAAAATAGAGCT+GGG	0.606882	4_1:+80795559	Msa0576730:three_prime_UTR
GATACCGTGTTCTTCCGGGA+AGG	0.607058	4_1:+80794853	Msa0576730:three_prime_UTR
TTGATCATGCCGGATGCGAG+TGG	0.612378	4_1:+80795769	Msa0576730:three_prime_UTR
AGCTCAAAACCAAGCACCAG+AGG	0.612912	4_1:+80794220	Msa0576730:CDS
AAGCACGCAGGTTTGTCGAA+TGG	0.614852	4_1:+80794701	Msa0576730:three_prime_UTR
ACTTGGTGGTGATATTGACT+TGG	0.615160	4_1:+80794977	Msa0576730:three_prime_UTR
GAGTGCTTTGCAAAGCACGC+AGG	0.616754	4_1:+80794689	Msa0576730:three_prime_UTR
AGATACTCTTCATCATCGCA+TGG	0.618697	4_1:-80794793	None:intergenic
TATGTAAAATTGGAGGTGAG+TGG	0.623493	4_1:+80794665	Msa0576730:three_prime_UTR
GGCTATAACAAACAGGAAAG+GGG	0.627042	4_1:+80795627	Msa0576730:three_prime_UTR
TAATGGTGAAACTAGCTTGG+TGG	0.627473	4_1:+80795705	Msa0576730:three_prime_UTR
CCATATGATTGGGCAAGCTG+AGG	0.633601	4_1:+80794831	Msa0576730:three_prime_UTR
CAAAACCAAGCACCAGAGGT+CGG	0.635046	4_1:+80794224	Msa0576730:CDS
AAAACCTTCCCGGAAGAACA+CGG	0.639566	4_1:-80794857	None:intergenic
GATTCTTCATAGTCATGTAG+TGG	0.655238	4_1:+80792640	Msa0576730:five_prime_UTR
ATGACACAACAGTGAACATG+TGG	0.658159	4_1:-80796059	None:intergenic
TATAGCCCAAATGTTAGTGG+AGG	0.659042	4_1:-80792330	None:intergenic
GTTAGCATACTATCTTCCAA+GGG	0.673648	4_1:-80795743	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTATTATAGTTG+AGG	+	chr4_1:80792834-80792853	Msa0576730:intron	10.0%
!!!	GTCAATTTTTTATTATTTTT+TGG	+	chr4_1:80794897-80794916	Msa0576730:intron	10.0%
!!!	TTTATTTGTTATTACTATAT+AGG	+	chr4_1:80795344-80795363	Msa0576730:intron	10.0%
!!	ATACATAACAATGATAAAAT+AGG	-	chr4_1:80792738-80792757	None:intergenic	15.0%
!!	AATGTATAATATCATTTGTT+GGG	-	chr4_1:80794019-80794038	None:intergenic	15.0%
!!	TTGAAAATTATGTAAAAAGT+AGG	+	chr4_1:80794057-80794076	Msa0576730:intron	15.0%
!!	TGAAAATTATGTAAAAAGTA+GGG	+	chr4_1:80794058-80794077	Msa0576730:intron	15.0%
!!	TAATTGAAAGAGAAAAAAAA+AGG	-	chr4_1:80794358-80794377	None:intergenic	15.0%
!!	TATTAGTTACTATCATATTT+AGG	+	chr4_1:80795965-80795984	Msa0576730:three_prime_UTR	15.0%
!!	ATTAGTTACTATCATATTTA+GGG	+	chr4_1:80795966-80795985	Msa0576730:three_prime_UTR	15.0%
!!!	AATCAGGTTATTTAAATTTT+TGG	+	chr4_1:80796168-80796187	Msa0576730:three_prime_UTR	15.0%
!!!	TATTTTTCCAGAAATTCTTA+GGG	+	chr4_1:80792771-80792790	Msa0576730:intron	20.0%
!!	TATGAGTTTAAAAATATGAG+AGG	+	chr4_1:80792912-80792931	Msa0576730:intron	20.0%
!!!	TTGTTGTTCTTAGAATAAAA+AGG	+	chr4_1:80792973-80792992	Msa0576730:intron	20.0%
!!!	TTTTCAACTTTTTTTCTTTC+TGG	+	chr4_1:80793039-80793058	Msa0576730:intron	20.0%
!!!	TTTCAACTTTTTTTCTTTCT+GGG	+	chr4_1:80793040-80793059	Msa0576730:intron	20.0%
!!!	TTGAGAATTATTTTGGATTT+TGG	+	chr4_1:80793176-80793195	Msa0576730:intron	20.0%
!!	TCATAAAAAATCTCATATTC+AGG	-	chr4_1:80793240-80793259	None:intergenic	20.0%
!!!	TGAATATGAGATTTTTTATG+AGG	+	chr4_1:80793239-80793258	Msa0576730:intron	20.0%
!!	AGAAATTATCAATTGTTTGT+TGG	+	chr4_1:80793282-80793301	Msa0576730:intron	20.0%
!!	TCAAACAACAGAAAATTAAA+TGG	-	chr4_1:80793399-80793418	None:intergenic	20.0%
!!!	AATGTGTTGTTTTGATAAAA+AGG	+	chr4_1:80793486-80793505	Msa0576730:intron	20.0%
!!!	ATGTGTTGTTTTGATAAAAA+GGG	+	chr4_1:80793487-80793506	Msa0576730:intron	20.0%
!!!	TCATTTTTCAAGCAATTTTA+TGG	+	chr4_1:80793571-80793590	Msa0576730:CDS	20.0%
!!	TTGTCATCAGAATTATTTAA+TGG	+	chr4_1:80793661-80793680	Msa0576730:intron	20.0%
!!!	TTGTGATCAGAATTATTTTA+TGG	+	chr4_1:80793868-80793887	Msa0576730:intron	20.0%
!!	CAATGTATAATATCATTTGT+TGG	-	chr4_1:80794020-80794039	None:intergenic	20.0%
!!!	AATTACATTGATTACTTGAT+GGG	-	chr4_1:80795248-80795267	None:intergenic	20.0%
!!!	AAATTACATTGATTACTTGA+TGG	-	chr4_1:80795249-80795268	None:intergenic	20.0%
!!!	TGTAATTTGCTTATATGTTT+TGG	+	chr4_1:80795261-80795280	Msa0576730:intron	20.0%
!	TCCAATTGATGAAATTTCTT+TGG	+	chr4_1:80792302-80792321	Msa0576730:five_prime_UTR	25.0%
!	ACCAAAGAAATTTCATCAAT+TGG	-	chr4_1:80792306-80792325	None:intergenic	25.0%
!	TAACATTTGGGCTATATATA+TGG	+	chr4_1:80792337-80792356	Msa0576730:five_prime_UTR	25.0%
!	AAATTCGATTTACTGTTCAT+GGG	-	chr4_1:80792464-80792483	None:intergenic	25.0%
!!	CATGATTTTCAATTGATCAA+AGG	-	chr4_1:80792693-80792712	None:intergenic	25.0%
!!	GTATTTTTCCAGAAATTCTT+AGG	+	chr4_1:80792770-80792789	Msa0576730:intron	25.0%
!!!	GATTTCTTGGTTTATTTTGT+TGG	+	chr4_1:80792861-80792880	Msa0576730:intron	25.0%
!	CATAATTCATGTACACAAAA+CGG	-	chr4_1:80792896-80792915	None:intergenic	25.0%
!	AGTTTAAAAATATGAGAGGA+AGG	+	chr4_1:80792916-80792935	Msa0576730:intron	25.0%
!	GTTTAAAAATATGAGAGGAA+GGG	+	chr4_1:80792917-80792936	Msa0576730:intron	25.0%
!	TTGTTCTTAGAATAAAAAGG+AGG	+	chr4_1:80792976-80792995	Msa0576730:intron	25.0%
!!!	TTCAACTTTTTTTCTTTCTG+GGG	+	chr4_1:80793041-80793060	Msa0576730:intron	25.0%
!!	TTGTTGTTGAGAATTAAGAA+CGG	+	chr4_1:80793089-80793108	Msa0576730:intron	25.0%
!!	TGCAGAATTGAGAATTATTT+TGG	+	chr4_1:80793169-80793188	Msa0576730:intron	25.0%
!!!	ATTTTGGATTTTGGTTACTT+TGG	+	chr4_1:80793185-80793204	Msa0576730:intron	25.0%
!!!	TGATCGATTTTTAATCTCTT+TGG	+	chr4_1:80793321-80793340	Msa0576730:intron	25.0%
!!	TTATGTAAAAAGTAGGGTTT+TGG	+	chr4_1:80794064-80794083	Msa0576730:intron	25.0%
!!	TATGTAAAAAGTAGGGTTTT+GGG	+	chr4_1:80794065-80794084	Msa0576730:intron	25.0%
!	AAGAAATAAGCTAAGAATGA+AGG	-	chr4_1:80794110-80794129	None:intergenic	25.0%
!!	TTATTTCTTTATTCCGTACA+GGG	+	chr4_1:80794121-80794140	Msa0576730:intron	25.0%
!	ATCATCATCATAACTGTTAA+TGG	+	chr4_1:80794492-80794511	Msa0576730:intron	25.0%
!!	ATCACTTTCATTGATTATTG+TGG	+	chr4_1:80794592-80794611	Msa0576730:three_prime_UTR	25.0%
!!!	TCTAATTCTTGTTGTTACTA+AGG	+	chr4_1:80794729-80794748	Msa0576730:three_prime_UTR	25.0%
!!!	ATTTTTTGGTTCTGATATGT+TGG	+	chr4_1:80794911-80794930	Msa0576730:intron	25.0%
!	TTTATGTCTTAAGCTAGTAA+AGG	-	chr4_1:80795013-80795032	None:intergenic	25.0%
!!	TTACTAGCTTAAGACATAAA+TGG	+	chr4_1:80795013-80795032	Msa0576730:three_prime_UTR	25.0%
!!	GAAGTCTTCTAAATTAGTTT+AGG	+	chr4_1:80795114-80795133	Msa0576730:intron	25.0%
!	AGATACACATGAAAAAATGA+GGG	-	chr4_1:80795299-80795318	None:intergenic	25.0%
!	TAGATACACATGAAAAAATG+AGG	-	chr4_1:80795300-80795319	None:intergenic	25.0%
!	TCATGTGTATCTATTTAACT+TGG	+	chr4_1:80795307-80795326	Msa0576730:intron	25.0%
!!!	GTATCTATTTAACTTGGTTT+TGG	+	chr4_1:80795313-80795332	Msa0576730:intron	25.0%
!	TGATGATTATTACACTTAAG+TGG	-	chr4_1:80795854-80795873	None:intergenic	25.0%
!	TGAAATTTAAGTGTAGAATC+AGG	+	chr4_1:80796152-80796171	Msa0576730:three_prime_UTR	25.0%
!	AGAACAGTATACATGTAAAT+GGG	-	chr4_1:80796216-80796235	None:intergenic	25.0%
!	AAGAACAGTATACATGTAAA+TGG	-	chr4_1:80796217-80796236	None:intergenic	25.0%
!	CGGAAAAATGGAAATTAATA+TGG	-	chr4_1:80796251-80796270	None:intergenic	25.0%
	GGCAAAAATGGAATTATCTA+GGG	-	chr4_1:80792247-80792266	None:intergenic	30.0%
	ATATATAGCCCAAATGTTAG+TGG	-	chr4_1:80792336-80792355	None:intergenic	30.0%
!!	TTCTAAAGACTGGTATGTTT+TGG	-	chr4_1:80792439-80792458	None:intergenic	30.0%
	AATTCGATTTACTGTTCATG+GGG	-	chr4_1:80792463-80792482	None:intergenic	30.0%
	CAAATTCGATTTACTGTTCA+TGG	-	chr4_1:80792465-80792484	None:intergenic	30.0%
	TTTCAATTGATCAAAGGATG+AGG	-	chr4_1:80792687-80792706	None:intergenic	30.0%
	AGGAAACACAAAAATTTGAC+TGG	-	chr4_1:80792718-80792737	None:intergenic	30.0%
	AACATAACCCTAAGAATTTC+TGG	-	chr4_1:80792781-80792800	None:intergenic	30.0%
!!!	TAGTTGAGGTTTTGATTTCT+TGG	+	chr4_1:80792848-80792867	Msa0576730:intron	30.0%
!!	GGTAATTTGCTAATCCTTAA+TGG	+	chr4_1:80792997-80793016	Msa0576730:intron	30.0%
!	TAATCCTTAATGGATGTGTT+TGG	+	chr4_1:80793007-80793026	Msa0576730:intron	30.0%
	AATTCCAAACACATCCATTA+AGG	-	chr4_1:80793014-80793033	None:intergenic	30.0%
!	CTTTGTGTACAAATTTTTGC+TGG	+	chr4_1:80793123-80793142	Msa0576730:intron	30.0%
	TGAGATGAAAATTATGTGCT+TGG	+	chr4_1:80793367-80793386	Msa0576730:intron	30.0%
	GAGATGAAAATTATGTGCTT+GGG	+	chr4_1:80793368-80793387	Msa0576730:intron	30.0%
!!	GTTTTGATAAAAAGGGAATC+AGG	+	chr4_1:80793494-80793513	Msa0576730:intron	30.0%
	TGAAAATGTGATCACAATCA+TGG	+	chr4_1:80793522-80793541	Msa0576730:exon	30.0%
	GAAAATGTGATCACAATCAT+GGG	+	chr4_1:80793523-80793542	Msa0576730:exon	30.0%
!	ATTAAGGCTTGGTTTCATTA+AGG	+	chr4_1:80793621-80793640	Msa0576730:CDS	30.0%
	AAAGCCAACAAATGACATTA+AGG	+	chr4_1:80793812-80793831	Msa0576730:intron	30.0%
!	ATTAAGGCTTGGTTTCATTA+AGG	+	chr4_1:80793621-80793640	Msa0576730:CDS	30.0%
!	ATCGTTCTTCTTTTTGTTCT+AGG	+	chr4_1:80793917-80793936	Msa0576730:intron	30.0%
!	CTTATTTCTTTATTCCGTAC+AGG	+	chr4_1:80794120-80794139	Msa0576730:intron	30.0%
	AAGAGAAAAAAAAAGGTGAG+AGG	-	chr4_1:80794351-80794370	None:intergenic	30.0%
	AAGTCTCTGGTATGTAAAAT+TGG	+	chr4_1:80794655-80794674	Msa0576730:three_prime_UTR	30.0%
!!	ATTTATCTGTGATTTGTTCG+TGG	+	chr4_1:80794769-80794788	Msa0576730:three_prime_UTR	30.0%
	TCTGTGAATAACCATATGAT+TGG	+	chr4_1:80794820-80794839	Msa0576730:three_prime_UTR	30.0%
	CTGTGAATAACCATATGATT+GGG	+	chr4_1:80794821-80794840	Msa0576730:three_prime_UTR	30.0%
!	ATGACACTAATATCCTTTTC+CGG	+	chr4_1:80794943-80794962	Msa0576730:intron	30.0%
	GTGATGAATTATCTTTCAGT+TGG	+	chr4_1:80795153-80795172	Msa0576730:intron	30.0%
	TATAAACCAAGAGTGGAATT+TGG	-	chr4_1:80795206-80795225	None:intergenic	30.0%
	TCCACTCTTGGTTTATATTA+AGG	+	chr4_1:80795209-80795228	Msa0576730:intron	30.0%
	ACCTTAATATAAACCAAGAG+TGG	-	chr4_1:80795213-80795232	None:intergenic	30.0%
	GTATATCATTTCACAGAACA+AGG	+	chr4_1:80795387-80795406	Msa0576730:intron	30.0%
	ACATTCTTTGACAAAACAGT+TGG	-	chr4_1:80795422-80795441	None:intergenic	30.0%
!	AACTGTTTTGTCAAAGAATG+TGG	+	chr4_1:80795421-80795440	Msa0576730:three_prime_UTR	30.0%
!	ATGACATTGATATTCTGGTT+TGG	-	chr4_1:80795481-80795500	None:intergenic	30.0%
!	AAAACGAGAAGGTCTATTTT+CGG	+	chr4_1:80795515-80795534	Msa0576730:three_prime_UTR	30.0%
	CCATTATAATCAATGAACGT+TGG	-	chr4_1:80795691-80795710	None:intergenic	30.0%
!	CCAACGTTCATTGATTATAA+TGG	+	chr4_1:80795688-80795707	Msa0576730:three_prime_UTR	30.0%
	TTATAATGGTGAAACTAGCT+TGG	+	chr4_1:80795702-80795721	Msa0576730:three_prime_UTR	30.0%
!	GAAGAACAATACTTTGCTTT+TGG	+	chr4_1:80795817-80795836	Msa0576730:three_prime_UTR	30.0%
!	TACTATCATATTTAGGGTCA+TGG	+	chr4_1:80795972-80795991	Msa0576730:three_prime_UTR	30.0%
!	TGCACTTTGTTGAATTGTTT+TGG	+	chr4_1:80796011-80796030	Msa0576730:three_prime_UTR	30.0%
	TGTGTCATGGTTTATTGTAA+TGG	+	chr4_1:80796074-80796093	Msa0576730:three_prime_UTR	30.0%
	GGGCAAAAATGGAATTATCT+AGG	-	chr4_1:80792248-80792267	None:intergenic	35.0%
!!	TTCCATTTTTGCCCTCATTT+TGG	+	chr4_1:80792254-80792273	Msa0576730:five_prime_UTR	35.0%
!!	TCCATTTTTGCCCTCATTTT+GGG	+	chr4_1:80792255-80792274	Msa0576730:five_prime_UTR	35.0%
	TCATTTCCTTCTTCAGATTG+TGG	+	chr4_1:80792365-80792384	Msa0576730:five_prime_UTR	35.0%
	ATGAAACCACAATCTGAAGA+AGG	-	chr4_1:80792374-80792393	None:intergenic	35.0%
!!	TTCTGAGATTGGTTTGAGTT+GGG	+	chr4_1:80792586-80792605	Msa0576730:five_prime_UTR	35.0%
	GATTCTTCATAGTCATGTAG+TGG	+	chr4_1:80792640-80792659	Msa0576730:five_prime_UTR	35.0%
	GCTTGCATGTCTTTACAAAA+AGG	+	chr4_1:80792809-80792828	Msa0576730:intron	35.0%
	AGTTGAACTATGCGACATTA+AGG	+	chr4_1:80793605-80793624	Msa0576730:CDS	35.0%
	ACGTAAGTATGATCAATGAC+TGG	+	chr4_1:80793695-80793714	Msa0576730:intron	35.0%
!	GGTAACGTTCTTTTTGTTCT+AGG	+	chr4_1:80793716-80793735	Msa0576730:intron	35.0%
!!	TTTGTTCTAGGAACGTGTTA+AGG	+	chr4_1:80793728-80793747	Msa0576730:intron	35.0%
	AGTGAACTGTGAATATTCCT+TGG	-	chr4_1:80793769-80793788	None:intergenic	35.0%
!!	ATTTGTTGGCTTTACCTCAA+AGG	-	chr4_1:80793805-80793824	None:intergenic	35.0%
!	CAAGCCTTAATGTCATTTGT+TGG	-	chr4_1:80793819-80793838	None:intergenic	35.0%
	CAACAAATGACATTAAGGCT+TGG	+	chr4_1:80793817-80793836	Msa0576730:intron	35.0%
	AGAATAGTATGATCAGTGAC+TGG	+	chr4_1:80793893-80793912	Msa0576730:intron	35.0%
!!	TTTGTTCTAGGAACGTGTTA+AGG	+	chr4_1:80793728-80793747	Msa0576730:intron	35.0%
	AGTGAATTGTGAAGATTCCT+TGG	-	chr4_1:80793970-80793989	None:intergenic	35.0%
	GAATCTTCACAATTCACTCT+TGG	+	chr4_1:80793972-80793991	Msa0576730:CDS	35.0%
!	ATTTGTTGGGTTTACCTCAA+AGG	-	chr4_1:80794006-80794025	None:intergenic	35.0%
	AGAGAAGACTAATATCGTGA+TGG	-	chr4_1:80794279-80794298	None:intergenic	35.0%
!!	CACGATATTAGTCTTCTCTT+TGG	+	chr4_1:80794280-80794299	Msa0576730:intron	35.0%
	AGAAGCTAACAAATCTGCAA+AGG	-	chr4_1:80794318-80794337	None:intergenic	35.0%
	ATTTATGAGCGTTATGAGAC+TGG	+	chr4_1:80794405-80794424	Msa0576730:intron	35.0%
	TCTCTGGTATGTAAAATTGG+AGG	+	chr4_1:80794658-80794677	Msa0576730:three_prime_UTR	35.0%
	TATGTAAAATTGGAGGTGAG+TGG	+	chr4_1:80794665-80794684	Msa0576730:three_prime_UTR	35.0%
	ATGTAAAATTGGAGGTGAGT+GGG	+	chr4_1:80794666-80794685	Msa0576730:three_prime_UTR	35.0%
!!	TTCTTGTTGTTACTAAGGAG+AGG	+	chr4_1:80794734-80794753	Msa0576730:three_prime_UTR	35.0%
!!	TCTTGTTGTTACTAAGGAGA+GGG	+	chr4_1:80794735-80794754	Msa0576730:three_prime_UTR	35.0%
!!!	ATAGGCTAGCTTTTTTGACA+TGG	+	chr4_1:80795362-80795381	Msa0576730:intron	35.0%
	CCTGAATGACATTGATATTC+TGG	-	chr4_1:80795486-80795505	None:intergenic	35.0%
	CCAGAATATCAATGTCATTC+AGG	+	chr4_1:80795483-80795502	Msa0576730:three_prime_UTR	35.0%
!!	GGTTGATAAGAAAAACGAGA+AGG	+	chr4_1:80795504-80795523	Msa0576730:three_prime_UTR	35.0%
	AGAGTTCTACAAAATAGAGC+TGG	+	chr4_1:80795558-80795577	Msa0576730:three_prime_UTR	35.0%
	GAGTTCTACAAAATAGAGCT+GGG	+	chr4_1:80795559-80795578	Msa0576730:three_prime_UTR	35.0%
	GTATCTGAATCTACTCCTTA+TGG	+	chr4_1:80795606-80795625	Msa0576730:three_prime_UTR	35.0%
	TCCTTATGGCTATAACAAAC+AGG	+	chr4_1:80795620-80795639	Msa0576730:three_prime_UTR	35.0%
!!	TCCTGTTTGTTATAGCCATA+AGG	-	chr4_1:80795624-80795643	None:intergenic	35.0%
	ATGGCTATAACAAACAGGAA+AGG	+	chr4_1:80795625-80795644	Msa0576730:three_prime_UTR	35.0%
	TGGCTATAACAAACAGGAAA+GGG	+	chr4_1:80795626-80795645	Msa0576730:three_prime_UTR	35.0%
!	TAGCTGGAATGGAATTTGTT+GGG	-	chr4_1:80795666-80795685	None:intergenic	35.0%
	GTTAGCATACTATCTTCCAA+GGG	-	chr4_1:80795746-80795765	None:intergenic	35.0%
	AGTTAGCATACTATCTTCCA+AGG	-	chr4_1:80795747-80795766	None:intergenic	35.0%
!!	ACAATACTTTGCTTTTGGTG+AGG	+	chr4_1:80795822-80795841	Msa0576730:three_prime_UTR	35.0%
	CTGATACATGTATCTCCTAT+TGG	-	chr4_1:80795903-80795922	None:intergenic	35.0%
	CAATAGGAGATACATGTATC+AGG	+	chr4_1:80795901-80795920	Msa0576730:three_prime_UTR	35.0%
	TAATATATCCTGCTCTTCAC+TGG	-	chr4_1:80795950-80795969	None:intergenic	35.0%
	TGCAGCAATAATTCAAAGAC+AGG	-	chr4_1:80796102-80796121	None:intergenic	35.0%
	TGACACCTCCACTAACATTT+GGG	+	chr4_1:80792325-80792344	Msa0576730:five_prime_UTR	40.0%
	TATAGCCCAAATGTTAGTGG+AGG	-	chr4_1:80792333-80792352	None:intergenic	40.0%
!	TTCATGGGGTTTCTAAAGAC+TGG	-	chr4_1:80792449-80792468	None:intergenic	40.0%
	TCGATGAACAGTAATGCGAT+AGG	+	chr4_1:80792562-80792581	Msa0576730:five_prime_UTR	40.0%
	CGATGAACAGTAATGCGATA+GGG	+	chr4_1:80792563-80792582	Msa0576730:five_prime_UTR	40.0%
!!	GTTCTGAGATTGGTTTGAGT+TGG	+	chr4_1:80792585-80792604	Msa0576730:five_prime_UTR	40.0%
	GTTCTTTGATCTGCTGTTGT+TGG	+	chr4_1:80792608-80792627	Msa0576730:five_prime_UTR	40.0%
	CATGTACACAAAACGGAATC+AGG	-	chr4_1:80792889-80792908	None:intergenic	40.0%
	AACTATGCGACATTAAGGCT+TGG	+	chr4_1:80793610-80793629	Msa0576730:CDS	40.0%
	GAATATTCACAGTTCACTCC+TGG	+	chr4_1:80793771-80793790	Msa0576730:intron	40.0%
!!!	TAAAAAGTAGGGTTTTGGGC+AGG	+	chr4_1:80794069-80794088	Msa0576730:intron	40.0%
!	TATTCCGTACAGGGTTTTCA+AGG	+	chr4_1:80794130-80794149	Msa0576730:intron	40.0%
!	TTTTGAGCTTGATGTTGGCA+TGG	-	chr4_1:80794209-80794228	None:intergenic	40.0%
!!!	CTTGGTTTTGAGCTTGATGT+TGG	-	chr4_1:80794214-80794233	None:intergenic	40.0%
	AGACTGGTGTACATCTATGA+TGG	+	chr4_1:80794421-80794440	Msa0576730:intron	40.0%
	TGTAAAATTGGAGGTGAGTG+GGG	+	chr4_1:80794667-80794686	Msa0576730:three_prime_UTR	40.0%
	AGATACTCTTCATCATCGCA+TGG	-	chr4_1:80794796-80794815	None:intergenic	40.0%
	TCAATATCACCACCAAGTTC+TGG	-	chr4_1:80794975-80794994	None:intergenic	40.0%
!!	ACTTGGTGGTGATATTGACT+TGG	+	chr4_1:80794977-80794996	Msa0576730:three_prime_UTR	40.0%
	GACTATCCAAATTCCACTCT+TGG	+	chr4_1:80795197-80795216	Msa0576730:intron	40.0%
	CATGAAAAAATGAGGGTCGA+GGG	-	chr4_1:80795292-80795311	None:intergenic	40.0%
	ACATGAAAAAATGAGGGTCG+AGG	-	chr4_1:80795293-80795312	None:intergenic	40.0%
	TTCACAGAACAAGGCTAGAA+TGG	+	chr4_1:80795396-80795415	Msa0576730:intron	40.0%
	GATTCAGATACGCATATGTC+AGG	-	chr4_1:80795597-80795616	None:intergenic	40.0%
	GGCTATAACAAACAGGAAAG+GGG	+	chr4_1:80795627-80795646	Msa0576730:three_prime_UTR	40.0%
!!	TGGAATGGAATTTGTTGGGT+AGG	-	chr4_1:80795662-80795681	None:intergenic	40.0%
	GTAGCTGGAATGGAATTTGT+TGG	-	chr4_1:80795667-80795686	None:intergenic	40.0%
!	TAATGGTGAAACTAGCTTGG+TGG	+	chr4_1:80795705-80795724	Msa0576730:three_prime_UTR	40.0%
!!	TGCTAACTTGTTGATCATGC+CGG	+	chr4_1:80795759-80795778	Msa0576730:three_prime_UTR	40.0%
	GTGTTTGTGAAGTCCATTGA+CGG	+	chr4_1:80795875-80795894	Msa0576730:three_prime_UTR	40.0%
	ATGACACAACAGTGAACATG+TGG	-	chr4_1:80796062-80796081	None:intergenic	40.0%
	ACATGTTCACTGTTGTGTCA+TGG	+	chr4_1:80796061-80796080	Msa0576730:three_prime_UTR	40.0%
	GCCCAAAATGAGGGCAAAAA+TGG	-	chr4_1:80792259-80792278	None:intergenic	45.0%
!	CCCTAAAGTGCCCAAAATGA+GGG	-	chr4_1:80792268-80792287	None:intergenic	45.0%
!	CCCTCATTTTGGGCACTTTA+GGG	+	chr4_1:80792265-80792284	Msa0576730:five_prime_UTR	45.0%
!	CCTCATTTTGGGCACTTTAG+GGG	+	chr4_1:80792266-80792285	Msa0576730:five_prime_UTR	45.0%
	GTGACACCTCCACTAACATT+TGG	+	chr4_1:80792324-80792343	Msa0576730:five_prime_UTR	45.0%
!!	ATGCGATAGGGTTCTGAGAT+TGG	+	chr4_1:80792575-80792594	Msa0576730:five_prime_UTR	45.0%
!	GTCATGTAGTGGTGCAGTTT+TGG	+	chr4_1:80792651-80792670	Msa0576730:five_prime_UTR	45.0%
!!!	TGTAGTGGTGCAGTTTTGGA+AGG	+	chr4_1:80792655-80792674	Msa0576730:five_prime_UTR	45.0%
	TCTGCCTTGAAAACCCTGTA+CGG	-	chr4_1:80794137-80794156	None:intergenic	45.0%
	TCATCATCGCTGAAGGAAAC+CGG	-	chr4_1:80794181-80794200	None:intergenic	45.0%
!	TTTTCACAGAAGCCTGACCA+TGG	-	chr4_1:80794547-80794566	None:intergenic	45.0%
	AAAACCTTCCCGGAAGAACA+CGG	-	chr4_1:80794860-80794879	None:intergenic	45.0%
	AGAGCAAGACAAAACCTTCC+CGG	-	chr4_1:80794870-80794889	None:intergenic	45.0%
!	GAGAAGGTCTATTTTCGGCA+GGG	+	chr4_1:80795520-80795539	Msa0576730:three_prime_UTR	45.0%
	TCAATGAACGTTGGTGTAGC+TGG	-	chr4_1:80795682-80795701	None:intergenic	45.0%
!	TATTTAGGGTCATGGTGTGC+AGG	+	chr4_1:80795980-80795999	Msa0576730:three_prime_UTR	45.0%
!	ATTTAGGGTCATGGTGTGCA+GGG	+	chr4_1:80795981-80796000	Msa0576730:three_prime_UTR	45.0%
	CTGCACTCTCTGAACTAATC+AGG	+	chr4_1:80796117-80796136	Msa0576730:three_prime_UTR	45.0%
!	GCCCTCATTTTGGGCACTTT+AGG	+	chr4_1:80792264-80792283	Msa0576730:five_prime_UTR	50.0%
!	CCCCTAAAGTGCCCAAAATG+AGG	-	chr4_1:80792269-80792288	None:intergenic	50.0%
!!!	TGGGCACTTTAGGGGCATTT+TGG	+	chr4_1:80792274-80792293	Msa0576730:five_prime_UTR	50.0%
!!	TCTTGGTGCTCAGTCCTTTG+AGG	+	chr4_1:80793989-80794008	Msa0576730:CDS	50.0%
!	TTTCAAGGCAGAAGTGCAGC+TGG	+	chr4_1:80794145-80794164	Msa0576730:CDS	50.0%
	GGCATCTTCATCATCGCTGA+AGG	-	chr4_1:80794188-80794207	None:intergenic	50.0%
	AGCTCAAAACCAAGCACCAG+AGG	+	chr4_1:80794220-80794239	Msa0576730:CDS	50.0%
	CAAAACCAAGCACCAGAGGT+CGG	+	chr4_1:80794224-80794243	Msa0576730:CDS	50.0%
	AACAGATCCTTTCCGACCTC+TGG	-	chr4_1:80794239-80794258	None:intergenic	50.0%
	TGTGCAAGTACGCAAGTCTC+TGG	+	chr4_1:80794642-80794661	Msa0576730:three_prime_UTR	50.0%
	AAGCACGCAGGTTTGTCGAA+TGG	+	chr4_1:80794701-80794720	Msa0576730:three_prime_UTR	50.0%
	CCTCAGCTTGCCCAATCATA+TGG	-	chr4_1:80794834-80794853	None:intergenic	50.0%
	CCATATGATTGGGCAAGCTG+AGG	+	chr4_1:80794831-80794850	Msa0576730:three_prime_UTR	50.0%
	CTGAGGATACCGTGTTCTTC+CGG	+	chr4_1:80794848-80794867	Msa0576730:three_prime_UTR	50.0%
	TGAGGATACCGTGTTCTTCC+GGG	+	chr4_1:80794849-80794868	Msa0576730:three_prime_UTR	50.0%
	GTTCTGGTGCAAACCGGAAA+AGG	-	chr4_1:80794959-80794978	None:intergenic	50.0%
!	TTCCGGTTTGCACCAGAACT+TGG	+	chr4_1:80794960-80794979	Msa0576730:intron	50.0%
!	CACCAAGTTCTGGTGCAAAC+CGG	-	chr4_1:80794965-80794984	None:intergenic	50.0%
!	CGAGAAGGTCTATTTTCGGC+AGG	+	chr4_1:80795519-80795538	Msa0576730:three_prime_UTR	50.0%
!	AGGTCTATTTTCGGCAGGGT+TGG	+	chr4_1:80795524-80795543	Msa0576730:three_prime_UTR	50.0%
	ACAAAATAGAGCTGGGTGCC+TGG	+	chr4_1:80795566-80795585	Msa0576730:three_prime_UTR	50.0%
	CGCATATGTCAGGCAAATCC+AGG	-	chr4_1:80795587-80795606	None:intergenic	50.0%
	GAACGTTGGTGTAGCTGGAA+TGG	-	chr4_1:80795677-80795696	None:intergenic	50.0%
	GTGTCAGCATGCATTTCCCT+TGG	+	chr4_1:80795727-80795746	Msa0576730:three_prime_UTR	50.0%
!	AGTCCATTGACGGTGCCAAT+AGG	+	chr4_1:80795885-80795904	Msa0576730:three_prime_UTR	50.0%
	TCTCCTATTGGCACCGTCAA+TGG	-	chr4_1:80795891-80795910	None:intergenic	50.0%
	CTGGACAACGAATGAGATGC+AGG	-	chr4_1:80795931-80795950	None:intergenic	50.0%
	TTCGTTGTCCAGTGAAGAGC+AGG	+	chr4_1:80795939-80795958	Msa0576730:three_prime_UTR	50.0%
	TCCTGGTGCTCAGTCCTTTG+AGG	+	chr4_1:80793788-80793807	Msa0576730:intron	55.0%
	ACCTCAAAGGACTGAGCACC+AGG	-	chr4_1:80793792-80793811	None:intergenic	55.0%
	TGCAGCTGGAGCTGATAGTC+CGG	+	chr4_1:80794159-80794178	Msa0576730:CDS	55.0%
	TTTCAACGCAGCTCCTGCCA+TGG	+	chr4_1:80794527-80794546	Msa0576730:intron	55.0%
	GAGTGCTTTGCAAAGCACGC+AGG	+	chr4_1:80794689-80794708	Msa0576730:three_prime_UTR	55.0%
	GATACCGTGTTCTTCCGGGA+AGG	+	chr4_1:80794853-80794872	Msa0576730:three_prime_UTR	55.0%
	CGGTTTGCACCAGAACTTGG+TGG	+	chr4_1:80794963-80794982	Msa0576730:intron	55.0%
	TTGATCATGCCGGATGCGAG+TGG	+	chr4_1:80795769-80795788	Msa0576730:three_prime_UTR	55.0%
	CTCACATCTCCACTCGCATC+CGG	-	chr4_1:80795781-80795800	None:intergenic	55.0%
	GGAGTGATCGACACGCTCCA+AGG	+	chr4_1:80793749-80793768	Msa0576730:intron	60.0%
	CCTTTCCGACCTCTGGTGCT+TGG	-	chr4_1:80794232-80794251	None:intergenic	60.0%
	CCAAGCACCAGAGGTCGGAA+AGG	+	chr4_1:80794229-80794248	Msa0576730:CDS	60.0%
	CACAGAAGCCTGACCATGGC+AGG	-	chr4_1:80794543-80794562	None:intergenic	60.0%
	AGGGTCATGGTGTGCAGGGT+TGG	+	chr4_1:80795985-80796004	Msa0576730:three_prime_UTR	60.0%
	GGAGCGATCTACGCGCTCCA+AGG	+	chr4_1:80793950-80793969	Msa0576730:CDS	65.0%
	ACGCAGCTCCTGCCATGGTC+AGG	+	chr4_1:80794532-80794551	Msa0576730:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	80792243	80796274	80792243	ID=Msa0576730;Name=Msa0576730
chr4_1	mRNA	80792243	80796274	80792243	ID=Msa0576730-mRNA-1;Parent=Msa0576730;Name=Msa0576730-mRNA-1;_AED=0.19;_eAED=0.19;_QI=467\|0.83\|1\|1\|0.66\|0.57\|7\|1263\|108
chr4_1	exon	80792243	80792676	80792243	ID=Msa0576730-mRNA-1:exon:29037;Parent=Msa0576730-mRNA-1
chr4_1	exon	80793508	80793642	80793508	ID=Msa0576730-mRNA-1:exon:29038;Parent=Msa0576730-mRNA-1
chr4_1	exon	80793939	80794010	80793939	ID=Msa0576730-mRNA-1:exon:29039;Parent=Msa0576730-mRNA-1
chr4_1	exon	80794142	80794268	80794142	ID=Msa0576730-mRNA-1:exon:29040;Parent=Msa0576730-mRNA-1
chr4_1	exon	80794538	80794874	80794538	ID=Msa0576730-mRNA-1:exon:29041;Parent=Msa0576730-mRNA-1
chr4_1	exon	80794976	80795055	80794976	ID=Msa0576730-mRNA-1:exon:29042;Parent=Msa0576730-mRNA-1
chr4_1	exon	80795403	80796274	80795403	ID=Msa0576730-mRNA-1:exon:29043;Parent=Msa0576730-mRNA-1
chr4_1	five_prime_UTR	80792243	80792676	80792243	ID=Msa0576730-mRNA-1:five_prime_utr;Parent=Msa0576730-mRNA-1
chr4_1	five_prime_UTR	80793508	80793540	80793508	ID=Msa0576730-mRNA-1:five_prime_utr;Parent=Msa0576730-mRNA-1
chr4_1	CDS	80793541	80793642	80793541	ID=Msa0576730-mRNA-1:cds;Parent=Msa0576730-mRNA-1
chr4_1	CDS	80793939	80794010	80793939	ID=Msa0576730-mRNA-1:cds;Parent=Msa0576730-mRNA-1
chr4_1	CDS	80794142	80794268	80794142	ID=Msa0576730-mRNA-1:cds;Parent=Msa0576730-mRNA-1
chr4_1	CDS	80794538	80794563	80794538	ID=Msa0576730-mRNA-1:cds;Parent=Msa0576730-mRNA-1
chr4_1	three_prime_UTR	80794564	80794874	80794564	ID=Msa0576730-mRNA-1:three_prime_utr;Parent=Msa0576730-mRNA-1
chr4_1	three_prime_UTR	80794976	80795055	80794976	ID=Msa0576730-mRNA-1:three_prime_utr;Parent=Msa0576730-mRNA-1
chr4_1	three_prime_UTR	80795403	80796274	80795403	ID=Msa0576730-mRNA-1:three_prime_utr;Parent=Msa0576730-mRNA-1

Gene Sequence	>Msa0576730 ATGGGTTTAGAAGCTATTTTATTTCAGAAATCATTTTTCAAGCAATTTTATGGATCTGTGACAAAGTTGAACTATGCGACATTAAGGCTTGGTTTCATTAAGGAACGTGTTAAGGAGCGATCTACGCGCTCCAAGGAATCTTCACAATTCACTCTTGGTGCTCAGTCCTTTGAGGGTTTTCAAGGCAGAAGTGCAGCTGGAGCTGATAGTCCGGTTTCCTTCAGCGATGATGAAGATGCCATGCCAACATCAAGCTCAAAACCAAGCACCAGAGGTCGGAAAGGATCTGTTGCATCTAATTCTCCTGCCATGGTCAGGCTTCTGTGA
Protein sequence	>Msa0576730 MGLEAILFQKSFFKQFYGSVTKLNYATLRLGFIKERVKERSTRSKESSQFTLGAQSFEGFQGRSAAGADSPVSFSDDEDAMPTSSSKPSTRGRKGSVASNSPAMVRLL*