AlfalfaGEDB | Msa0702270

M. sativa cultivar zhongmu-4 / Msa0702270

KEGG: K10865
GO: GO:0000228 GO:0000785 GO:0000791 GO:0000793 GO:0000794 GO:0000795 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0099086
PFAM: PF03094.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0702270	RHN75196.1	88.235	68	8	0	34	101	498	565	1.63e-28	119

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0702270	A0A396JA88	88.235	68	8	0	34	101	498	565	7.79e-29	119

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008090	Msa0702270	0.802689	4.979975e-49	-8.615850e-47
Msa0020390	Msa0702270	0.803983	2.686083e-49	-8.615850e-47
Msa0026660	Msa0702270	0.803432	3.495841e-49	-8.615850e-47
Msa0027740	Msa0702270	0.852590	4.179053e-61	-8.615850e-47
Msa0033880	Msa0702270	0.809362	1.964434e-50	-8.615850e-47
Msa0049390	Msa0702270	0.825544	4.424031e-54	-8.615850e-47
Msa0053690	Msa0702270	0.810048	1.399085e-50	-8.615850e-47
Msa0053700	Msa0702270	0.822147	2.767388e-53	-8.615850e-47
Msa0055100	Msa0702270	0.814934	1.196024e-51	-8.615850e-47
Msa0062120	Msa0702270	0.804978	1.666136e-49	-8.615850e-47
Msa0065010	Msa0702270	0.807848	4.135902e-50	-8.615850e-47
Msa0068020	Msa0702270	0.809874	1.524857e-50	-8.615850e-47
Msa0070170	Msa0702270	0.808581	2.887019e-50	-8.615850e-47
Msa0097590	Msa0702270	0.804004	2.659329e-49	-8.615850e-47
Msa0097610	Msa0702270	0.803614	3.205409e-49	-8.615850e-47
Msa0101180	Msa0702270	0.833123	6.388222e-56	-8.615850e-47
Msa0108870	Msa0702270	0.804148	2.482291e-49	-8.615850e-47
Msa0115370	Msa0702270	0.800950	1.133062e-48	-8.615850e-47
Msa0118900	Msa0702270	0.801892	7.265173e-49	-8.615850e-47
Msa0603670	Msa0702270	0.801829	7.485440e-49	-8.615850e-47
Msa0619920	Msa0702270	0.801627	8.235059e-49	-8.615850e-47
Msa0626940	Msa0702270	0.800560	1.360855e-48	-8.615850e-47
Msa0628420	Msa0702270	0.815736	7.936386e-52	-8.615850e-47
Msa0647430	Msa0702270	0.801872	7.334750e-49	-8.615850e-47
Msa0662630	Msa0702270	0.812181	4.824346e-51	-8.615850e-47
Msa0679360	Msa0702270	0.801526	8.636296e-49	-8.615850e-47
Msa0687230	Msa0702270	0.816160	6.381030e-52	-8.615850e-47
Msa0689500	Msa0702270	0.800320	1.522975e-48	-8.615850e-47
Msa0699190	Msa0702270	0.824868	6.393815e-54	-8.615850e-47
Msa0699780	Msa0702270	0.801448	8.960485e-49	-8.615850e-47
Msa0702150	Msa0702270	0.957304	3.745383e-115	-8.615850e-47
Msa0702270	Msa0703910	0.801603	8.329423e-49	-8.615850e-47
Msa0702270	Msa0705390	0.822784	1.968031e-53	-8.615850e-47
Msa0702270	Msa0748920	0.814579	1.433979e-51	-8.615850e-47
Msa0702270	Msa0751010	0.803501	3.383164e-49	-8.615850e-47
Msa0702270	Msa0764690	0.806849	6.735351e-50	-8.615850e-47
Msa0702270	Msa0792780	0.828680	7.857603e-55	-8.615850e-47
Msa0702270	Msa0794050	0.818925	1.519382e-52	-8.615850e-47
Msa0702270	Msa0799480	0.806111	9.640843e-50	-8.615850e-47
Msa0702270	Msa0895310	0.809716	1.649028e-50	-8.615850e-47
Msa0702270	Msa0901110	0.811653	6.285616e-51	-8.615850e-47
Msa0702270	Msa0903780	0.809781	1.597205e-50	-8.615850e-47
Msa0702270	Msa0943270	0.808576	2.893633e-50	-8.615850e-47
Msa0702270	Msa0944200	0.801546	8.557077e-49	-8.615850e-47
Msa0702270	Msa0990830	0.813925	1.999612e-51	-8.615850e-47
Msa0702270	Msa1012540	0.808316	3.287788e-50	-8.615850e-47
Msa0702270	Msa1020100	0.801816	7.532940e-49	-8.615850e-47
Msa0702270	Msa1025260	0.807772	4.293257e-50	-8.615850e-47
Msa0702270	Msa1027250	0.800315	1.526576e-48	-8.615850e-47
Msa0702270	Msa1059310	0.812168	4.855897e-51	-8.615850e-47
Msa0702270	Msa1067070	0.833112	6.428357e-56	-8.615850e-47
Msa0702270	Msa1083880	0.813468	2.520642e-51	-8.615850e-47
Msa0702270	Msa1109350	0.802281	6.044214e-49	-8.615850e-47
Msa0702270	Msa1126850	0.816732	4.752073e-52	-8.615850e-47
Msa0702270	Msa1131560	0.802356	5.833214e-49	-8.615850e-47
Msa0702270	Msa1154490	0.805369	1.379883e-49	-8.615850e-47
Msa0702270	Msa1173900	0.829613	4.667708e-55	-8.615850e-47
Msa0702270	Msa1176360	0.812299	4.546274e-51	-8.615850e-47
Msa0702270	Msa1185440	0.802307	5.968496e-49	-8.615850e-47
Msa0702270	Msa1202580	0.808426	3.115256e-50	-8.615850e-47
Msa0702270	Msa1205850	0.811434	7.014475e-51	-8.615850e-47
Msa0702270	Msa1206310	0.806562	7.745498e-50	-8.615850e-47
Msa0702270	Msa1211830	0.845155	4.843076e-59	-8.615850e-47
Msa0702270	Msa1253400	0.823392	1.420070e-53	-8.615850e-47
Msa0702270	Msa1253810	0.845534	3.825145e-59	-8.615850e-47
Msa0702270	Msa1258370	0.812925	3.317352e-51	-8.615850e-47
Msa0702270	Msa1259640	0.804883	1.744172e-49	-8.615850e-47
Msa0702270	Msa1265590	0.827055	1.932736e-54	-8.615850e-47
Msa0702270	Msa1278900	0.826853	2.160252e-54	-8.615850e-47
Msa0702270	Msa1279590	0.833535	5.043346e-56	-8.615850e-47
Msa0702270	Msa1289300	0.837361	5.439754e-57	-8.615850e-47
Msa0702270	Msa1297600	0.842044	3.287326e-58	-8.615850e-47
Msa0702270	Msa1299700	0.825894	3.654400e-54	-8.615850e-47
Msa0702270	Msa1303580	0.812695	3.725021e-51	-8.615850e-47
Msa0702270	Msa1307180	0.806590	7.638653e-50	-8.615850e-47
Msa0702270	Msa1316360	0.801039	1.086693e-48	-8.615850e-47
Msa0702270	Msa1320580	0.812213	4.746860e-51	-8.615850e-47
Msa0702270	Msa1333970	0.813809	2.120880e-51	-8.615850e-47
Msa0702270	Msa1351540	0.806239	9.058560e-50	-8.615850e-47
Msa0702270	Msa1372310	0.810694	1.014745e-50	-8.615850e-47
Msa0702270	Msa1381480	0.808745	2.663182e-50	-8.615850e-47
Msa0702270	Msa1381650	0.811128	8.173522e-51	-8.615850e-47
Msa0702270	Msa1411950	0.824440	8.063562e-54	-8.615850e-47
Msa0702270	Msa1433600	0.816011	6.887933e-52	-8.615850e-47
Msa0702270	Msa1434340	0.805246	1.464699e-49	-8.615850e-47
Msa0702270	Msa1437270	0.810190	1.303575e-50	-8.615850e-47
Msa0702270	Msa1445940	0.820784	5.710587e-53	-8.615850e-47
Msa0702270	Msa1447890	0.802093	6.606254e-49	-8.615850e-47
Msa0702270	Msa1452570	0.814463	1.520822e-51	-8.615850e-47
Msa0702270	Msa1455280	0.834439	2.995596e-56	-8.615850e-47
Msa0702270	Msa1457220	0.810765	9.796867e-51	-8.615850e-47
Msa0126770	Msa0702270	0.826513	2.603268e-54	-8.615850e-47
Msa0126840	Msa0702270	0.812100	5.024951e-51	-8.615850e-47
Msa0126880	Msa0702270	0.810169	1.317504e-50	-8.615850e-47
Msa0127850	Msa0702270	0.803678	3.108966e-49	-8.615850e-47
Msa0164370	Msa0702270	0.839158	1.873602e-57	-8.615850e-47
Msa0166750	Msa0702270	0.800408	1.461730e-48	-8.615850e-47
Msa0167980	Msa0702270	0.817556	3.101340e-52	-8.615850e-47
Msa0168190	Msa0702270	0.869491	2.974958e-66	-8.615850e-47
Msa0174000	Msa0702270	0.818314	2.091204e-52	-8.615850e-47
Msa0175200	Msa0702270	0.836313	1.007119e-56	-8.615850e-47
Msa0183870	Msa0702270	0.825348	4.923212e-54	-8.615850e-47
Msa0207410	Msa0702270	0.821801	3.328214e-53	-8.615850e-47
Msa0207440	Msa0702270	0.809094	2.242118e-50	-8.615850e-47
Msa0364990	Msa0702270	0.849570	2.970133e-60	-8.615850e-47
Msa0379570	Msa0702270	0.805978	1.028304e-49	-8.615850e-47
Msa0382370	Msa0702270	0.803341	3.650400e-49	-8.615850e-47
Msa0384670	Msa0702270	0.820487	6.682694e-53	-8.615850e-47
Msa0394280	Msa0702270	0.840063	1.089201e-57	-8.615850e-47
Msa0414020	Msa0702270	0.834231	3.377175e-56	-8.615850e-47
Msa0450480	Msa0702270	0.808360	3.217789e-50	-8.615850e-47
Msa0458640	Msa0702270	0.846442	2.166513e-59	-8.615850e-47
Msa0482180	Msa0702270	0.808678	2.752119e-50	-8.615850e-47
Msa0257490	Msa0702270	0.810651	1.036936e-50	-8.615850e-47
Msa0296790	Msa0702270	0.821354	4.222144e-53	-8.615850e-47
Msa0296920	Msa0702270	0.807262	5.508596e-50	-8.615850e-47
Msa0304660	Msa0702270	0.810811	9.575364e-51	-8.615850e-47
Msa0331700	Msa0702270	0.810127	1.345384e-50	-8.615850e-47
Msa0337500	Msa0702270	0.802606	5.179400e-49	-8.615850e-47
Msa0337510	Msa0702270	0.837415	5.267780e-57	-8.615850e-47
Msa0511770	Msa0702270	0.804587	2.011151e-49	-8.615850e-47
Msa0527910	Msa0702270	0.815179	1.055262e-51	-8.615850e-47
Msa0530100	Msa0702270	0.819660	1.033331e-52	-8.615850e-47
Msa0531250	Msa0702270	0.810574	1.077352e-50	-8.615850e-47
Msa0539050	Msa0702270	0.836815	7.502713e-57	-8.615850e-47
Msa0539060	Msa0702270	0.833643	4.741424e-56	-8.615850e-47
Msa0550540	Msa0702270	0.804447	2.151198e-49	-8.615850e-47
Msa0550570	Msa0702270	0.805400	1.359732e-49	-8.615850e-47
Msa0576730	Msa0702270	0.985537	7.117310e-164	-8.615850e-47
Msa0580180	Msa0702270	0.804518	2.078944e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0702270	MtrunA17_Chr2g0318541	88.235	68	8	0	34	101	498	565	1.50e-32	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 131 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTAGTTACTATCATATTT+AGG	0.140806	4_4:+73838298	Msa0702270:three_prime_UTR
CCTGAATGACATTGATATTC+TGG	0.189504	4_4:-73837816	None:intergenic
AAGAACAGTATACATGTAAA+TGG	0.205118	4_4:-73838547	None:intergenic
TGGGCACTTTAGGGGCATTT+TGG	0.228149	4_4:+73834606	Msa0702270:five_prime_UTR
ATTAGTTACTATCATATTTA+GGG	0.265584	4_4:+73838299	Msa0702270:three_prime_UTR
TTCTAAAGACTGGTATGTTT+TGG	0.266573	4_4:-73834768	None:intergenic
TGCACTTTGTTGAATTGTTT+TGG	0.271540	4_4:+73838344	Msa0702270:three_prime_UTR
TCCAATTGATGAAATTTCTT+TGG	0.273796	4_4:+73834634	Msa0702270:five_prime_UTR
TTACTAGCTTAAGACATAAA+TGG	0.278661	4_4:+73837346	Msa0702270:three_prime_UTR
ATTAAGGCTTGGTTTCATTA+AGG	0.279842	4_4:+73835954	Msa0702270:CDS
CAAATTCGATTTACTGTTCA+TGG	0.283346	4_4:-73834794	None:intergenic
GAAGAACAATACTTTGCTTT+TGG	0.289019	4_4:+73838150	Msa0702270:three_prime_UTR
GTCATGTAGTGGTGCAGTTT+TGG	0.298095	4_4:+73834983	Msa0702270:five_prime_UTR
ACCAAAGAAATTTCATCAAT+TGG	0.303256	4_4:-73834635	None:intergenic
CCAACGTTCATTGATTATAA+TGG	0.304555	4_4:+73838021	Msa0702270:three_prime_UTR
GTGACACCTCCACTAACATT+TGG	0.310417	4_4:+73834656	Msa0702270:five_prime_UTR
CCAGAATATCAATGTCATTC+AGG	0.310628	4_4:+73837816	Msa0702270:three_prime_UTR
CTGAGGATACCGTGTTCTTC+CGG	0.313280	4_4:+73837181	Msa0702270:three_prime_UTR
AGTTGAACTATGCGACATTA+AGG	0.318921	4_4:+73835938	Msa0702270:CDS
TAACATTTGGGCTATATATA+TGG	0.319105	4_4:+73834669	Msa0702270:five_prime_UTR
TCTAATTCTTGTTGTTACTA+AGG	0.369203	4_4:+73837062	Msa0702270:three_prime_UTR
TTCTGAGATTGGTTTGAGTT+GGG	0.378227	4_4:+73834918	Msa0702270:five_prime_UTR
ATGCGATAGGGTTCTGAGAT+TGG	0.378475	4_4:+73834907	Msa0702270:five_prime_UTR
TGAAATTTAAGTGTAGAATC+AGG	0.382944	4_4:+73838485	Msa0702270:three_prime_UTR
CCTTTCCGACCTCTGGTGCT+TGG	0.384030	4_4:-73836562	None:intergenic
GTTCTTTGATCTGCTGTTGT+TGG	0.385533	4_4:+73834940	Msa0702270:five_prime_UTR
TCAATGAACGTTGGTGTAGC+TGG	0.388479	4_4:-73838012	None:intergenic
TGAGGATACCGTGTTCTTCC+GGG	0.388485	4_4:+73837182	Msa0702270:three_prime_UTR
CTGATACATGTATCTCCTAT+TGG	0.389711	4_4:-73838233	None:intergenic
GTATCTGAATCTACTCCTTA+TGG	0.395535	4_4:+73837939	Msa0702270:three_prime_UTR
TCCTGTTTGTTATAGCCATA+AGG	0.397282	4_4:-73837954	None:intergenic
TCTTGGTGCTCAGTCCTTTG+AGG	0.398508	4_4:+73836322	Msa0702270:CDS
ACAAAATAGAGCTGGGTGCC+TGG	0.399485	4_4:+73837899	Msa0702270:three_prime_UTR
AAGTCTCTGGTATGTAAAAT+TGG	0.405635	4_4:+73836988	Msa0702270:three_prime_UTR
TCTGTGAATAACCATATGAT+TGG	0.408436	4_4:+73837153	Msa0702270:three_prime_UTR
TGAAAATGTGATCACAATCA+TGG	0.410902	4_4:+73835855	Msa0702270:exon
GTTCTGAGATTGGTTTGAGT+TGG	0.411370	4_4:+73834917	Msa0702270:five_prime_UTR
CTGCACTCTCTGAACTAATC+AGG	0.411911	4_4:+73838450	Msa0702270:three_prime_UTR
TGTGTCATGGTTTATTGTAA+TGG	0.412851	4_4:+73838407	Msa0702270:three_prime_UTR
TCTCCTATTGGCACCGTCAA+TGG	0.417525	4_4:-73838221	None:intergenic
AACAGATCCTTTCCGACCTC+TGG	0.421775	4_4:-73836569	None:intergenic
ATGACATTGATATTCTGGTT+TGG	0.423705	4_4:-73837811	None:intergenic
TGACACCTCCACTAACATTT+GGG	0.425550	4_4:+73834657	Msa0702270:five_prime_UTR
TATTTAGGGTCATGGTGTGC+AGG	0.430442	4_4:+73838313	Msa0702270:three_prime_UTR
ACGCAGCTCCTGCCATGGTC+AGG	0.431291	4_4:+73836865	Msa0702270:intron
TGGCTATAACAAACAGGAAA+GGG	0.435466	4_4:+73837959	Msa0702270:three_prime_UTR
AGTGAATTGTGAAGATTCCT+TGG	0.446636	4_4:-73836300	None:intergenic
TAATATATCCTGCTCTTCAC+TGG	0.447204	4_4:-73838280	None:intergenic
CCTCAGCTTGCCCAATCATA+TGG	0.450278	4_4:-73837164	None:intergenic
TGATGATTATTACACTTAAG+TGG	0.450769	4_4:-73838184	None:intergenic
AGGGTCATGGTGTGCAGGGT+TGG	0.456110	4_4:+73838318	Msa0702270:three_prime_UTR
TCATTTCCTTCTTCAGATTG+TGG	0.458373	4_4:+73834697	Msa0702270:five_prime_UTR
TCATCATCGCTGAAGGAAAC+CGG	0.458878	4_4:-73836511	None:intergenic
CACAGAAGCCTGACCATGGC+AGG	0.462409	4_4:-73836873	None:intergenic
ATCACTTTCATTGATTATTG+TGG	0.462962	4_4:+73836925	Msa0702270:three_prime_UTR
CAATAGGAGATACATGTATC+AGG	0.463833	4_4:+73838234	Msa0702270:three_prime_UTR
CGCATATGTCAGGCAAATCC+AGG	0.467174	4_4:-73837917	None:intergenic
TTCACAGAACAAGGCTAGAA+TGG	0.468394	4_4:+73837729	Msa0702270:intron
GAATCTTCACAATTCACTCT+TGG	0.470201	4_4:+73836305	Msa0702270:CDS
TCCTTATGGCTATAACAAAC+AGG	0.471089	4_4:+73837953	Msa0702270:three_prime_UTR
TACTATCATATTTAGGGTCA+TGG	0.476367	4_4:+73838305	Msa0702270:three_prime_UTR
TTCATGGGGTTTCTAAAGAC+TGG	0.477011	4_4:-73834778	None:intergenic
ATTTATCTGTGATTTGTTCG+TGG	0.478617	4_4:+73837102	Msa0702270:three_prime_UTR
AGTCCATTGACGGTGCCAAT+AGG	0.481728	4_4:+73838218	Msa0702270:three_prime_UTR
TTCGTTGTCCAGTGAAGAGC+AGG	0.484634	4_4:+73838272	Msa0702270:three_prime_UTR
TGTGCAAGTACGCAAGTCTC+TGG	0.485815	4_4:+73836975	Msa0702270:three_prime_UTR
TGCAGCTGGAGCTGATAGTC+CGG	0.486818	4_4:+73836492	Msa0702270:CDS
CCCCTAAAGTGCCCAAAATG+AGG	0.487598	4_4:-73834598	None:intergenic
GTGTCAGCATGCATTTCCCT+TGG	0.489277	4_4:+73838060	Msa0702270:three_prime_UTR
CTGTGAATAACCATATGATT+GGG	0.489653	4_4:+73837154	Msa0702270:three_prime_UTR
TTTGTTCTAGGAACGTGTTA+AGG	0.492658	4_4:+73836262	Msa0702270:intron
AGAGCAAGACAAAACCTTCC+CGG	0.495380	4_4:-73837200	None:intergenic
AACTATGCGACATTAAGGCT+TGG	0.498825	4_4:+73835943	Msa0702270:CDS
TGCAGCAATAATTCAAAGAC+AGG	0.499476	4_4:-73838432	None:intergenic
AGAACAGTATACATGTAAAT+GGG	0.499738	4_4:-73838546	None:intergenic
AGAGTTCTACAAAATAGAGC+TGG	0.500119	4_4:+73837891	Msa0702270:three_prime_UTR
ATATATAGCCCAAATGTTAG+TGG	0.502458	4_4:-73834665	None:intergenic
TTTCAAGGCAGAAGTGCAGC+TGG	0.505124	4_4:+73836478	Msa0702270:CDS
CGGTTTGCACCAGAACTTGG+TGG	0.505268	4_4:+73837296	Msa0702270:intron
GATTCAGATACGCATATGTC+AGG	0.506496	4_4:-73837927	None:intergenic
ATTTAGGGTCATGGTGTGCA+GGG	0.511669	4_4:+73838314	Msa0702270:three_prime_UTR
AAATTCGATTTACTGTTCAT+GGG	0.512240	4_4:-73834793	None:intergenic
TCTTGTTGTTACTAAGGAGA+GGG	0.512640	4_4:+73837068	Msa0702270:three_prime_UTR
ATTTGTTGGGTTTACCTCAA+AGG	0.513130	4_4:-73836336	None:intergenic
ACATTCTTTGACAAAACAGT+TGG	0.514565	4_4:-73837752	None:intergenic
GAAAATGTGATCACAATCAT+GGG	0.515424	4_4:+73835856	Msa0702270:exon
TGGAATGGAATTTGTTGGGT+AGG	0.516364	4_4:-73837992	None:intergenic
TGTAAAATTGGAGGTGAGTG+GGG	0.520147	4_4:+73837000	Msa0702270:three_prime_UTR
TTTATGTCTTAAGCTAGTAA+AGG	0.520978	4_4:-73837343	None:intergenic
ATGTAAAATTGGAGGTGAGT+GGG	0.523440	4_4:+73836999	Msa0702270:three_prime_UTR
TAGCTGGAATGGAATTTGTT+GGG	0.523834	4_4:-73837996	None:intergenic
GTAGCTGGAATGGAATTTGT+TGG	0.537737	4_4:-73837997	None:intergenic
CTGGACAACGAATGAGATGC+AGG	0.540409	4_4:-73838261	None:intergenic
GAACGTTGGTGTAGCTGGAA+TGG	0.542196	4_4:-73838007	None:intergenic
ATGAAACCACAATCTGAAGA+AGG	0.543903	4_4:-73834703	None:intergenic
AGTTAGCATACTATCTTCCA+AGG	0.544019	4_4:-73838077	None:intergenic
CTCACATCTCCACTCGCATC+CGG	0.551685	4_4:-73838111	None:intergenic
ACATGTTCACTGTTGTGTCA+TGG	0.552124	4_4:+73838394	Msa0702270:three_prime_UTR
GTGTTTGTGAAGTCCATTGA+CGG	0.552423	4_4:+73838208	Msa0702270:three_prime_UTR
CGATGAACAGTAATGCGATA+GGG	0.553516	4_4:+73834895	Msa0702270:five_prime_UTR
GGAGCGATCTACGCGCTCCA+AGG	0.554334	4_4:+73836283	Msa0702270:CDS
ATGGCTATAACAAACAGGAA+AGG	0.562348	4_4:+73837958	Msa0702270:three_prime_UTR
TTCTTGTTGTTACTAAGGAG+AGG	0.564790	4_4:+73837067	Msa0702270:three_prime_UTR
TTTCAACGCAGCTCCTGCCA+TGG	0.567402	4_4:+73836860	Msa0702270:intron
GGCATCTTCATCATCGCTGA+AGG	0.569407	4_4:-73836518	None:intergenic
TCGATGAACAGTAATGCGAT+AGG	0.572164	4_4:+73834894	Msa0702270:five_prime_UTR
CCCTAAAGTGCCCAAAATGA+GGG	0.576444	4_4:-73834597	None:intergenic
TTATAATGGTGAAACTAGCT+TGG	0.578050	4_4:+73838035	Msa0702270:three_prime_UTR
CCAAGCACCAGAGGTCGGAA+AGG	0.579149	4_4:+73836562	Msa0702270:CDS
TCTCTGGTATGTAAAATTGG+AGG	0.581652	4_4:+73836991	Msa0702270:three_prime_UTR
AATTCGATTTACTGTTCATG+GGG	0.602667	4_4:-73834792	None:intergenic
TGCTAACTTGTTGATCATGC+CGG	0.603742	4_4:+73838092	Msa0702270:three_prime_UTR
CCATTATAATCAATGAACGT+TGG	0.604275	4_4:-73838021	None:intergenic
GAGTTCTACAAAATAGAGCT+GGG	0.606882	4_4:+73837892	Msa0702270:three_prime_UTR
GATACCGTGTTCTTCCGGGA+AGG	0.607058	4_4:+73837186	Msa0702270:three_prime_UTR
TTGATCATGCCGGATGCGAG+TGG	0.612378	4_4:+73838102	Msa0702270:three_prime_UTR
AGCTCAAAACCAAGCACCAG+AGG	0.612912	4_4:+73836553	Msa0702270:CDS
AAGCACGCAGGTTTGTCGAA+TGG	0.614852	4_4:+73837034	Msa0702270:three_prime_UTR
ACTTGGTGGTGATATTGACT+TGG	0.615160	4_4:+73837310	Msa0702270:three_prime_UTR
GAGTGCTTTGCAAAGCACGC+AGG	0.616754	4_4:+73837022	Msa0702270:three_prime_UTR
AGATACTCTTCATCATCGCA+TGG	0.618697	4_4:-73837126	None:intergenic
TATGTAAAATTGGAGGTGAG+TGG	0.623493	4_4:+73836998	Msa0702270:three_prime_UTR
GGCTATAACAAACAGGAAAG+GGG	0.627042	4_4:+73837960	Msa0702270:three_prime_UTR
TAATGGTGAAACTAGCTTGG+TGG	0.627473	4_4:+73838038	Msa0702270:three_prime_UTR
CCATATGATTGGGCAAGCTG+AGG	0.633601	4_4:+73837164	Msa0702270:three_prime_UTR
CAAAACCAAGCACCAGAGGT+CGG	0.635046	4_4:+73836557	Msa0702270:CDS
AAAACCTTCCCGGAAGAACA+CGG	0.639566	4_4:-73837190	None:intergenic
GATTCTTCATAGTCATGTAG+TGG	0.655238	4_4:+73834972	Msa0702270:five_prime_UTR
ATGACACAACAGTGAACATG+TGG	0.658159	4_4:-73838392	None:intergenic
TATAGCCCAAATGTTAGTGG+AGG	0.659042	4_4:-73834662	None:intergenic
GTTAGCATACTATCTTCCAA+GGG	0.673648	4_4:-73838076	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTATTATAGTTG+AGG	+	chr4_4:73835166-73835185	Msa0702270:intron	10.0%
!!!	GTCAATTTTTTATTATTTTT+TGG	+	chr4_4:73837230-73837249	Msa0702270:intron	10.0%
!!!	TTTATTTGTTATTACTATAT+AGG	+	chr4_4:73837677-73837696	Msa0702270:intron	10.0%
!!	ATACATAACAATGATAAAAT+AGG	-	chr4_4:73835070-73835089	None:intergenic	15.0%
!!	AATGTATAATATCATTTGTT+GGG	-	chr4_4:73836352-73836371	None:intergenic	15.0%
!!	TTGAAAATTATGTAAAAAGT+AGG	+	chr4_4:73836390-73836409	Msa0702270:intron	15.0%
!!	TGAAAATTATGTAAAAAGTA+GGG	+	chr4_4:73836391-73836410	Msa0702270:intron	15.0%
!!	TAATTGAAAGAGAAAAAAAA+AGG	-	chr4_4:73836691-73836710	None:intergenic	15.0%
!!	TATTAGTTACTATCATATTT+AGG	+	chr4_4:73838298-73838317	Msa0702270:three_prime_UTR	15.0%
!!	ATTAGTTACTATCATATTTA+GGG	+	chr4_4:73838299-73838318	Msa0702270:three_prime_UTR	15.0%
!!!	AATCAGGTTATTTAAATTTT+TGG	+	chr4_4:73838501-73838520	Msa0702270:three_prime_UTR	15.0%
!!!	TATTTTTCCAGAAATTCTTA+GGG	+	chr4_4:73835103-73835122	Msa0702270:intron	20.0%
!!!	ATTTCTTGGTTTTATTTTGT+TGG	+	chr4_4:73835194-73835213	Msa0702270:intron	20.0%
!!	TATGAGTTTAAAAATATGAG+AGG	+	chr4_4:73835245-73835264	Msa0702270:intron	20.0%
!!!	TTGTTGTTCTTAGAATAAAA+AGG	+	chr4_4:73835306-73835325	Msa0702270:intron	20.0%
!!!	TTTTCAACTTTTTTTCTTTC+TGG	+	chr4_4:73835372-73835391	Msa0702270:intron	20.0%
!!!	TTTCAACTTTTTTTCTTTCT+GGG	+	chr4_4:73835373-73835392	Msa0702270:intron	20.0%
!!!	TTGAGAATTATTTTGGATTT+TGG	+	chr4_4:73835509-73835528	Msa0702270:intron	20.0%
!!	TCATAAAAAATCTCATATTC+AGG	-	chr4_4:73835573-73835592	None:intergenic	20.0%
!!!	TGAATATGAGATTTTTTATG+AGG	+	chr4_4:73835572-73835591	Msa0702270:intron	20.0%
!!	AGAAATTATCAATTGTTTGT+TGG	+	chr4_4:73835615-73835634	Msa0702270:intron	20.0%
!!	TCAAACAACAGAAAATTAAA+TGG	-	chr4_4:73835732-73835751	None:intergenic	20.0%
!!!	AATGTGTTGTTTTGATAAAA+AGG	+	chr4_4:73835819-73835838	Msa0702270:intron	20.0%
!!!	ATGTGTTGTTTTGATAAAAA+GGG	+	chr4_4:73835820-73835839	Msa0702270:intron	20.0%
!!!	TCATTTTTCAAGCAATTTTA+TGG	+	chr4_4:73835904-73835923	Msa0702270:CDS	20.0%
!!	TTGTCATCAGAATTATTTAA+TGG	+	chr4_4:73835994-73836013	Msa0702270:intron	20.0%
!!!	TTGTGATCAGAATTATTTTA+TGG	+	chr4_4:73836201-73836220	Msa0702270:intron	20.0%
!!	CAATGTATAATATCATTTGT+TGG	-	chr4_4:73836353-73836372	None:intergenic	20.0%
!!!	AATTACATTGATTACTTGAT+GGG	-	chr4_4:73837581-73837600	None:intergenic	20.0%
!!!	AAATTACATTGATTACTTGA+TGG	-	chr4_4:73837582-73837601	None:intergenic	20.0%
!!!	TGTAATTTGCTTATATGTTT+TGG	+	chr4_4:73837594-73837613	Msa0702270:intron	20.0%
!	TCCAATTGATGAAATTTCTT+TGG	+	chr4_4:73834634-73834653	Msa0702270:five_prime_UTR	25.0%
!	ACCAAAGAAATTTCATCAAT+TGG	-	chr4_4:73834638-73834657	None:intergenic	25.0%
!	TAACATTTGGGCTATATATA+TGG	+	chr4_4:73834669-73834688	Msa0702270:five_prime_UTR	25.0%
!	AAATTCGATTTACTGTTCAT+GGG	-	chr4_4:73834796-73834815	None:intergenic	25.0%
!!	CATGATTTTCAATTGATCAA+AGG	-	chr4_4:73835025-73835044	None:intergenic	25.0%
!!	GTATTTTTCCAGAAATTCTT+AGG	+	chr4_4:73835102-73835121	Msa0702270:intron	25.0%
!	CATAATTCATGTACACAAAA+CGG	-	chr4_4:73835229-73835248	None:intergenic	25.0%
!	AGTTTAAAAATATGAGAGGA+AGG	+	chr4_4:73835249-73835268	Msa0702270:intron	25.0%
!	GTTTAAAAATATGAGAGGAA+GGG	+	chr4_4:73835250-73835269	Msa0702270:intron	25.0%
!	TTGTTCTTAGAATAAAAAGG+AGG	+	chr4_4:73835309-73835328	Msa0702270:intron	25.0%
!!!	TTCAACTTTTTTTCTTTCTG+GGG	+	chr4_4:73835374-73835393	Msa0702270:intron	25.0%
!!	TTGTTGTTGAGAATTAAGAA+CGG	+	chr4_4:73835422-73835441	Msa0702270:intron	25.0%
!!	TGCAGAATTGAGAATTATTT+TGG	+	chr4_4:73835502-73835521	Msa0702270:intron	25.0%
!!!	ATTTTGGATTTTGGTTACTT+TGG	+	chr4_4:73835518-73835537	Msa0702270:intron	25.0%
!!!	TGATCGATTTTTAATCTCTT+TGG	+	chr4_4:73835654-73835673	Msa0702270:intron	25.0%
!!	TTATGTAAAAAGTAGGGTTT+TGG	+	chr4_4:73836397-73836416	Msa0702270:intron	25.0%
!!	TATGTAAAAAGTAGGGTTTT+GGG	+	chr4_4:73836398-73836417	Msa0702270:intron	25.0%
!	AAGAAATAAGCTAAGAATGA+AGG	-	chr4_4:73836443-73836462	None:intergenic	25.0%
!!	TTATTTCTTTATTCCGTACA+GGG	+	chr4_4:73836454-73836473	Msa0702270:intron	25.0%
!	ATCATCATCATAACTGTTAA+TGG	+	chr4_4:73836825-73836844	Msa0702270:intron	25.0%
!!	ATCACTTTCATTGATTATTG+TGG	+	chr4_4:73836925-73836944	Msa0702270:three_prime_UTR	25.0%
!!!	TCTAATTCTTGTTGTTACTA+AGG	+	chr4_4:73837062-73837081	Msa0702270:three_prime_UTR	25.0%
!!!	ATTTTTTGGTTCTGATATGT+TGG	+	chr4_4:73837244-73837263	Msa0702270:intron	25.0%
!	TTTATGTCTTAAGCTAGTAA+AGG	-	chr4_4:73837346-73837365	None:intergenic	25.0%
!!	TTACTAGCTTAAGACATAAA+TGG	+	chr4_4:73837346-73837365	Msa0702270:three_prime_UTR	25.0%
!!	GAAGTCTTCTAAATTAGTTT+AGG	+	chr4_4:73837447-73837466	Msa0702270:intron	25.0%
!	AGATACACATGAAAAAATGA+GGG	-	chr4_4:73837632-73837651	None:intergenic	25.0%
!	TAGATACACATGAAAAAATG+AGG	-	chr4_4:73837633-73837652	None:intergenic	25.0%
!	TCATGTGTATCTATTTAACT+TGG	+	chr4_4:73837640-73837659	Msa0702270:intron	25.0%
!!!	GTATCTATTTAACTTGGTTT+TGG	+	chr4_4:73837646-73837665	Msa0702270:intron	25.0%
!	TGATGATTATTACACTTAAG+TGG	-	chr4_4:73838187-73838206	None:intergenic	25.0%
!	TGAAATTTAAGTGTAGAATC+AGG	+	chr4_4:73838485-73838504	Msa0702270:three_prime_UTR	25.0%
!	AGAACAGTATACATGTAAAT+GGG	-	chr4_4:73838549-73838568	None:intergenic	25.0%
!	AAGAACAGTATACATGTAAA+TGG	-	chr4_4:73838550-73838569	None:intergenic	25.0%
!	CGGAAAAATGGAAATTAATA+TGG	-	chr4_4:73838584-73838603	None:intergenic	25.0%
	GGCAAAAATGGAATTATCTA+GGG	-	chr4_4:73834579-73834598	None:intergenic	30.0%
	ATATATAGCCCAAATGTTAG+TGG	-	chr4_4:73834668-73834687	None:intergenic	30.0%
!!	TTCTAAAGACTGGTATGTTT+TGG	-	chr4_4:73834771-73834790	None:intergenic	30.0%
	AATTCGATTTACTGTTCATG+GGG	-	chr4_4:73834795-73834814	None:intergenic	30.0%
	CAAATTCGATTTACTGTTCA+TGG	-	chr4_4:73834797-73834816	None:intergenic	30.0%
	TTTCAATTGATCAAAGGATG+AGG	-	chr4_4:73835019-73835038	None:intergenic	30.0%
	AGGAAACACAAAAATTTGAC+TGG	-	chr4_4:73835050-73835069	None:intergenic	30.0%
	AACATAACCCTAAGAATTTC+TGG	-	chr4_4:73835113-73835132	None:intergenic	30.0%
!!!	TAGTTGAGGTTTTGATTTCT+TGG	+	chr4_4:73835180-73835199	Msa0702270:intron	30.0%
!!	GGTAATTTGCTAATCCTTAA+TGG	+	chr4_4:73835330-73835349	Msa0702270:intron	30.0%
!	TAATCCTTAATGGATGTGTT+TGG	+	chr4_4:73835340-73835359	Msa0702270:intron	30.0%
	AATTCCAAACACATCCATTA+AGG	-	chr4_4:73835347-73835366	None:intergenic	30.0%
!	CTTTGTGTACAAATTTTTGC+TGG	+	chr4_4:73835456-73835475	Msa0702270:intron	30.0%
	TGAGATGAAAATTATGTGCT+TGG	+	chr4_4:73835700-73835719	Msa0702270:intron	30.0%
	GAGATGAAAATTATGTGCTT+GGG	+	chr4_4:73835701-73835720	Msa0702270:intron	30.0%
!!	GTTTTGATAAAAAGGGAATC+AGG	+	chr4_4:73835827-73835846	Msa0702270:intron	30.0%
	TGAAAATGTGATCACAATCA+TGG	+	chr4_4:73835855-73835874	Msa0702270:exon	30.0%
	GAAAATGTGATCACAATCAT+GGG	+	chr4_4:73835856-73835875	Msa0702270:exon	30.0%
!	ATTAAGGCTTGGTTTCATTA+AGG	+	chr4_4:73835954-73835973	Msa0702270:CDS	30.0%
	AAAGCCAACAAATGACATTA+AGG	+	chr4_4:73836145-73836164	Msa0702270:intron	30.0%
!	ATTAAGGCTTGGTTTCATTA+AGG	+	chr4_4:73835954-73835973	Msa0702270:CDS	30.0%
!	ATCGTTCTTCTTTTTGTTCT+AGG	+	chr4_4:73836250-73836269	Msa0702270:intron	30.0%
!	CTTATTTCTTTATTCCGTAC+AGG	+	chr4_4:73836453-73836472	Msa0702270:intron	30.0%
	AAGAGAAAAAAAAAGGTGAG+AGG	-	chr4_4:73836684-73836703	None:intergenic	30.0%
	AAGTCTCTGGTATGTAAAAT+TGG	+	chr4_4:73836988-73837007	Msa0702270:three_prime_UTR	30.0%
!!	ATTTATCTGTGATTTGTTCG+TGG	+	chr4_4:73837102-73837121	Msa0702270:three_prime_UTR	30.0%
	TCTGTGAATAACCATATGAT+TGG	+	chr4_4:73837153-73837172	Msa0702270:three_prime_UTR	30.0%
	CTGTGAATAACCATATGATT+GGG	+	chr4_4:73837154-73837173	Msa0702270:three_prime_UTR	30.0%
!	ATGACACTAATATCCTTTTC+CGG	+	chr4_4:73837276-73837295	Msa0702270:intron	30.0%
	GTGATGAATTATCTTTCAGT+TGG	+	chr4_4:73837486-73837505	Msa0702270:intron	30.0%
	TATAAACCAAGAGTGGAATT+TGG	-	chr4_4:73837539-73837558	None:intergenic	30.0%
	TCCACTCTTGGTTTATATTA+AGG	+	chr4_4:73837542-73837561	Msa0702270:intron	30.0%
	ACCTTAATATAAACCAAGAG+TGG	-	chr4_4:73837546-73837565	None:intergenic	30.0%
	GTATATCATTTCACAGAACA+AGG	+	chr4_4:73837720-73837739	Msa0702270:intron	30.0%
	ACATTCTTTGACAAAACAGT+TGG	-	chr4_4:73837755-73837774	None:intergenic	30.0%
!	AACTGTTTTGTCAAAGAATG+TGG	+	chr4_4:73837754-73837773	Msa0702270:three_prime_UTR	30.0%
!	ATGACATTGATATTCTGGTT+TGG	-	chr4_4:73837814-73837833	None:intergenic	30.0%
!	AAAACGAGAAGGTCTATTTT+CGG	+	chr4_4:73837848-73837867	Msa0702270:three_prime_UTR	30.0%
	CCATTATAATCAATGAACGT+TGG	-	chr4_4:73838024-73838043	None:intergenic	30.0%
!	CCAACGTTCATTGATTATAA+TGG	+	chr4_4:73838021-73838040	Msa0702270:three_prime_UTR	30.0%
	TTATAATGGTGAAACTAGCT+TGG	+	chr4_4:73838035-73838054	Msa0702270:three_prime_UTR	30.0%
!	GAAGAACAATACTTTGCTTT+TGG	+	chr4_4:73838150-73838169	Msa0702270:three_prime_UTR	30.0%
!	TACTATCATATTTAGGGTCA+TGG	+	chr4_4:73838305-73838324	Msa0702270:three_prime_UTR	30.0%
!	TGCACTTTGTTGAATTGTTT+TGG	+	chr4_4:73838344-73838363	Msa0702270:three_prime_UTR	30.0%
	TGTGTCATGGTTTATTGTAA+TGG	+	chr4_4:73838407-73838426	Msa0702270:three_prime_UTR	30.0%
	GGGCAAAAATGGAATTATCT+AGG	-	chr4_4:73834580-73834599	None:intergenic	35.0%
!!	TTCCATTTTTGCCCTCATTT+TGG	+	chr4_4:73834586-73834605	Msa0702270:five_prime_UTR	35.0%
!!	TCCATTTTTGCCCTCATTTT+GGG	+	chr4_4:73834587-73834606	Msa0702270:five_prime_UTR	35.0%
	TCATTTCCTTCTTCAGATTG+TGG	+	chr4_4:73834697-73834716	Msa0702270:five_prime_UTR	35.0%
	ATGAAACCACAATCTGAAGA+AGG	-	chr4_4:73834706-73834725	None:intergenic	35.0%
!!	TTCTGAGATTGGTTTGAGTT+GGG	+	chr4_4:73834918-73834937	Msa0702270:five_prime_UTR	35.0%
	GATTCTTCATAGTCATGTAG+TGG	+	chr4_4:73834972-73834991	Msa0702270:five_prime_UTR	35.0%
	GCTTGCATGTCTTTACAAAA+AGG	+	chr4_4:73835141-73835160	Msa0702270:intron	35.0%
	AGTTGAACTATGCGACATTA+AGG	+	chr4_4:73835938-73835957	Msa0702270:CDS	35.0%
	ACGTAAGTATGATCAATGAC+TGG	+	chr4_4:73836028-73836047	Msa0702270:intron	35.0%
!	GGTAACGTTCTTTTTGTTCT+AGG	+	chr4_4:73836049-73836068	Msa0702270:intron	35.0%
!!	TTTGTTCTAGGAACGTGTTA+AGG	+	chr4_4:73836061-73836080	Msa0702270:intron	35.0%
	AGTGAACTGTGAATATTCCT+TGG	-	chr4_4:73836102-73836121	None:intergenic	35.0%
!!	ATTTGTTGGCTTTACCTCAA+AGG	-	chr4_4:73836138-73836157	None:intergenic	35.0%
!	CAAGCCTTAATGTCATTTGT+TGG	-	chr4_4:73836152-73836171	None:intergenic	35.0%
	CAACAAATGACATTAAGGCT+TGG	+	chr4_4:73836150-73836169	Msa0702270:intron	35.0%
	AGAATAGTATGATCAGTGAC+TGG	+	chr4_4:73836226-73836245	Msa0702270:intron	35.0%
!!	TTTGTTCTAGGAACGTGTTA+AGG	+	chr4_4:73836061-73836080	Msa0702270:intron	35.0%
	AGTGAATTGTGAAGATTCCT+TGG	-	chr4_4:73836303-73836322	None:intergenic	35.0%
	GAATCTTCACAATTCACTCT+TGG	+	chr4_4:73836305-73836324	Msa0702270:CDS	35.0%
!	ATTTGTTGGGTTTACCTCAA+AGG	-	chr4_4:73836339-73836358	None:intergenic	35.0%
	AGAGAAGACTAATATCGTGA+TGG	-	chr4_4:73836612-73836631	None:intergenic	35.0%
!!	CACGATATTAGTCTTCTCTT+TGG	+	chr4_4:73836613-73836632	Msa0702270:intron	35.0%
	AGAAGCTAACAAATCTGCAA+AGG	-	chr4_4:73836651-73836670	None:intergenic	35.0%
	ATTTATGAGCGTTATGAGAC+TGG	+	chr4_4:73836738-73836757	Msa0702270:intron	35.0%
	TCTCTGGTATGTAAAATTGG+AGG	+	chr4_4:73836991-73837010	Msa0702270:three_prime_UTR	35.0%
	TATGTAAAATTGGAGGTGAG+TGG	+	chr4_4:73836998-73837017	Msa0702270:three_prime_UTR	35.0%
	ATGTAAAATTGGAGGTGAGT+GGG	+	chr4_4:73836999-73837018	Msa0702270:three_prime_UTR	35.0%
!!	TTCTTGTTGTTACTAAGGAG+AGG	+	chr4_4:73837067-73837086	Msa0702270:three_prime_UTR	35.0%
!!	TCTTGTTGTTACTAAGGAGA+GGG	+	chr4_4:73837068-73837087	Msa0702270:three_prime_UTR	35.0%
!!!	ATAGGCTAGCTTTTTTGACA+TGG	+	chr4_4:73837695-73837714	Msa0702270:intron	35.0%
	CCTGAATGACATTGATATTC+TGG	-	chr4_4:73837819-73837838	None:intergenic	35.0%
	CCAGAATATCAATGTCATTC+AGG	+	chr4_4:73837816-73837835	Msa0702270:three_prime_UTR	35.0%
!!	GGTTGATAAGAAAAACGAGA+AGG	+	chr4_4:73837837-73837856	Msa0702270:three_prime_UTR	35.0%
	AGAGTTCTACAAAATAGAGC+TGG	+	chr4_4:73837891-73837910	Msa0702270:three_prime_UTR	35.0%
	GAGTTCTACAAAATAGAGCT+GGG	+	chr4_4:73837892-73837911	Msa0702270:three_prime_UTR	35.0%
	GTATCTGAATCTACTCCTTA+TGG	+	chr4_4:73837939-73837958	Msa0702270:three_prime_UTR	35.0%
	TCCTTATGGCTATAACAAAC+AGG	+	chr4_4:73837953-73837972	Msa0702270:three_prime_UTR	35.0%
!!	TCCTGTTTGTTATAGCCATA+AGG	-	chr4_4:73837957-73837976	None:intergenic	35.0%
	ATGGCTATAACAAACAGGAA+AGG	+	chr4_4:73837958-73837977	Msa0702270:three_prime_UTR	35.0%
	TGGCTATAACAAACAGGAAA+GGG	+	chr4_4:73837959-73837978	Msa0702270:three_prime_UTR	35.0%
!	TAGCTGGAATGGAATTTGTT+GGG	-	chr4_4:73837999-73838018	None:intergenic	35.0%
	GTTAGCATACTATCTTCCAA+GGG	-	chr4_4:73838079-73838098	None:intergenic	35.0%
	AGTTAGCATACTATCTTCCA+AGG	-	chr4_4:73838080-73838099	None:intergenic	35.0%
!!	ACAATACTTTGCTTTTGGTG+AGG	+	chr4_4:73838155-73838174	Msa0702270:three_prime_UTR	35.0%
	CTGATACATGTATCTCCTAT+TGG	-	chr4_4:73838236-73838255	None:intergenic	35.0%
	CAATAGGAGATACATGTATC+AGG	+	chr4_4:73838234-73838253	Msa0702270:three_prime_UTR	35.0%
	TAATATATCCTGCTCTTCAC+TGG	-	chr4_4:73838283-73838302	None:intergenic	35.0%
	TGCAGCAATAATTCAAAGAC+AGG	-	chr4_4:73838435-73838454	None:intergenic	35.0%
	TGACACCTCCACTAACATTT+GGG	+	chr4_4:73834657-73834676	Msa0702270:five_prime_UTR	40.0%
	TATAGCCCAAATGTTAGTGG+AGG	-	chr4_4:73834665-73834684	None:intergenic	40.0%
!	TTCATGGGGTTTCTAAAGAC+TGG	-	chr4_4:73834781-73834800	None:intergenic	40.0%
	TCGATGAACAGTAATGCGAT+AGG	+	chr4_4:73834894-73834913	Msa0702270:five_prime_UTR	40.0%
	CGATGAACAGTAATGCGATA+GGG	+	chr4_4:73834895-73834914	Msa0702270:five_prime_UTR	40.0%
!!	GTTCTGAGATTGGTTTGAGT+TGG	+	chr4_4:73834917-73834936	Msa0702270:five_prime_UTR	40.0%
	GTTCTTTGATCTGCTGTTGT+TGG	+	chr4_4:73834940-73834959	Msa0702270:five_prime_UTR	40.0%
	CATGTACACAAAACGGAATC+AGG	-	chr4_4:73835222-73835241	None:intergenic	40.0%
	AACTATGCGACATTAAGGCT+TGG	+	chr4_4:73835943-73835962	Msa0702270:CDS	40.0%
	GAATATTCACAGTTCACTCC+TGG	+	chr4_4:73836104-73836123	Msa0702270:intron	40.0%
!!!	TAAAAAGTAGGGTTTTGGGC+AGG	+	chr4_4:73836402-73836421	Msa0702270:intron	40.0%
!	TATTCCGTACAGGGTTTTCA+AGG	+	chr4_4:73836463-73836482	Msa0702270:intron	40.0%
!	TTTTGAGCTTGATGTTGGCA+TGG	-	chr4_4:73836542-73836561	None:intergenic	40.0%
!!!	CTTGGTTTTGAGCTTGATGT+TGG	-	chr4_4:73836547-73836566	None:intergenic	40.0%
	AGACTGGTGTACATCTATGA+TGG	+	chr4_4:73836754-73836773	Msa0702270:intron	40.0%
	TGTAAAATTGGAGGTGAGTG+GGG	+	chr4_4:73837000-73837019	Msa0702270:three_prime_UTR	40.0%
	AGATACTCTTCATCATCGCA+TGG	-	chr4_4:73837129-73837148	None:intergenic	40.0%
	TCAATATCACCACCAAGTTC+TGG	-	chr4_4:73837308-73837327	None:intergenic	40.0%
!!	ACTTGGTGGTGATATTGACT+TGG	+	chr4_4:73837310-73837329	Msa0702270:three_prime_UTR	40.0%
	GACTATCCAAATTCCACTCT+TGG	+	chr4_4:73837530-73837549	Msa0702270:intron	40.0%
	CATGAAAAAATGAGGGTCGA+GGG	-	chr4_4:73837625-73837644	None:intergenic	40.0%
	ACATGAAAAAATGAGGGTCG+AGG	-	chr4_4:73837626-73837645	None:intergenic	40.0%
	TTCACAGAACAAGGCTAGAA+TGG	+	chr4_4:73837729-73837748	Msa0702270:intron	40.0%
	GATTCAGATACGCATATGTC+AGG	-	chr4_4:73837930-73837949	None:intergenic	40.0%
	GGCTATAACAAACAGGAAAG+GGG	+	chr4_4:73837960-73837979	Msa0702270:three_prime_UTR	40.0%
!!	TGGAATGGAATTTGTTGGGT+AGG	-	chr4_4:73837995-73838014	None:intergenic	40.0%
	GTAGCTGGAATGGAATTTGT+TGG	-	chr4_4:73838000-73838019	None:intergenic	40.0%
!	TAATGGTGAAACTAGCTTGG+TGG	+	chr4_4:73838038-73838057	Msa0702270:three_prime_UTR	40.0%
!!	TGCTAACTTGTTGATCATGC+CGG	+	chr4_4:73838092-73838111	Msa0702270:three_prime_UTR	40.0%
	GTGTTTGTGAAGTCCATTGA+CGG	+	chr4_4:73838208-73838227	Msa0702270:three_prime_UTR	40.0%
	ATGACACAACAGTGAACATG+TGG	-	chr4_4:73838395-73838414	None:intergenic	40.0%
	ACATGTTCACTGTTGTGTCA+TGG	+	chr4_4:73838394-73838413	Msa0702270:three_prime_UTR	40.0%
	GCCCAAAATGAGGGCAAAAA+TGG	-	chr4_4:73834591-73834610	None:intergenic	45.0%
!	CCCTAAAGTGCCCAAAATGA+GGG	-	chr4_4:73834600-73834619	None:intergenic	45.0%
!	CCCTCATTTTGGGCACTTTA+GGG	+	chr4_4:73834597-73834616	Msa0702270:five_prime_UTR	45.0%
!	CCTCATTTTGGGCACTTTAG+GGG	+	chr4_4:73834598-73834617	Msa0702270:five_prime_UTR	45.0%
	GTGACACCTCCACTAACATT+TGG	+	chr4_4:73834656-73834675	Msa0702270:five_prime_UTR	45.0%
!!	ATGCGATAGGGTTCTGAGAT+TGG	+	chr4_4:73834907-73834926	Msa0702270:five_prime_UTR	45.0%
!	GTCATGTAGTGGTGCAGTTT+TGG	+	chr4_4:73834983-73835002	Msa0702270:five_prime_UTR	45.0%
!!!	TGTAGTGGTGCAGTTTTGGA+AGG	+	chr4_4:73834987-73835006	Msa0702270:five_prime_UTR	45.0%
	TCTGCCTTGAAAACCCTGTA+CGG	-	chr4_4:73836470-73836489	None:intergenic	45.0%
	TCATCATCGCTGAAGGAAAC+CGG	-	chr4_4:73836514-73836533	None:intergenic	45.0%
!	TTTTCACAGAAGCCTGACCA+TGG	-	chr4_4:73836880-73836899	None:intergenic	45.0%
	AAAACCTTCCCGGAAGAACA+CGG	-	chr4_4:73837193-73837212	None:intergenic	45.0%
	AGAGCAAGACAAAACCTTCC+CGG	-	chr4_4:73837203-73837222	None:intergenic	45.0%
!	GAGAAGGTCTATTTTCGGCA+GGG	+	chr4_4:73837853-73837872	Msa0702270:three_prime_UTR	45.0%
	TCAATGAACGTTGGTGTAGC+TGG	-	chr4_4:73838015-73838034	None:intergenic	45.0%
!	TATTTAGGGTCATGGTGTGC+AGG	+	chr4_4:73838313-73838332	Msa0702270:three_prime_UTR	45.0%
!	ATTTAGGGTCATGGTGTGCA+GGG	+	chr4_4:73838314-73838333	Msa0702270:three_prime_UTR	45.0%
	CTGCACTCTCTGAACTAATC+AGG	+	chr4_4:73838450-73838469	Msa0702270:three_prime_UTR	45.0%
!	GCCCTCATTTTGGGCACTTT+AGG	+	chr4_4:73834596-73834615	Msa0702270:five_prime_UTR	50.0%
!	CCCCTAAAGTGCCCAAAATG+AGG	-	chr4_4:73834601-73834620	None:intergenic	50.0%
!!!	TGGGCACTTTAGGGGCATTT+TGG	+	chr4_4:73834606-73834625	Msa0702270:five_prime_UTR	50.0%
!!	TCTTGGTGCTCAGTCCTTTG+AGG	+	chr4_4:73836322-73836341	Msa0702270:CDS	50.0%
!	TTTCAAGGCAGAAGTGCAGC+TGG	+	chr4_4:73836478-73836497	Msa0702270:CDS	50.0%
	GGCATCTTCATCATCGCTGA+AGG	-	chr4_4:73836521-73836540	None:intergenic	50.0%
	AGCTCAAAACCAAGCACCAG+AGG	+	chr4_4:73836553-73836572	Msa0702270:CDS	50.0%
	CAAAACCAAGCACCAGAGGT+CGG	+	chr4_4:73836557-73836576	Msa0702270:CDS	50.0%
	AACAGATCCTTTCCGACCTC+TGG	-	chr4_4:73836572-73836591	None:intergenic	50.0%
	TGTGCAAGTACGCAAGTCTC+TGG	+	chr4_4:73836975-73836994	Msa0702270:three_prime_UTR	50.0%
	AAGCACGCAGGTTTGTCGAA+TGG	+	chr4_4:73837034-73837053	Msa0702270:three_prime_UTR	50.0%
	CCTCAGCTTGCCCAATCATA+TGG	-	chr4_4:73837167-73837186	None:intergenic	50.0%
	CCATATGATTGGGCAAGCTG+AGG	+	chr4_4:73837164-73837183	Msa0702270:three_prime_UTR	50.0%
	CTGAGGATACCGTGTTCTTC+CGG	+	chr4_4:73837181-73837200	Msa0702270:three_prime_UTR	50.0%
	TGAGGATACCGTGTTCTTCC+GGG	+	chr4_4:73837182-73837201	Msa0702270:three_prime_UTR	50.0%
	GTTCTGGTGCAAACCGGAAA+AGG	-	chr4_4:73837292-73837311	None:intergenic	50.0%
!	TTCCGGTTTGCACCAGAACT+TGG	+	chr4_4:73837293-73837312	Msa0702270:intron	50.0%
!	CACCAAGTTCTGGTGCAAAC+CGG	-	chr4_4:73837298-73837317	None:intergenic	50.0%
!	CGAGAAGGTCTATTTTCGGC+AGG	+	chr4_4:73837852-73837871	Msa0702270:three_prime_UTR	50.0%
!	AGGTCTATTTTCGGCAGGGT+TGG	+	chr4_4:73837857-73837876	Msa0702270:three_prime_UTR	50.0%
	ACAAAATAGAGCTGGGTGCC+TGG	+	chr4_4:73837899-73837918	Msa0702270:three_prime_UTR	50.0%
	CGCATATGTCAGGCAAATCC+AGG	-	chr4_4:73837920-73837939	None:intergenic	50.0%
	GAACGTTGGTGTAGCTGGAA+TGG	-	chr4_4:73838010-73838029	None:intergenic	50.0%
	GTGTCAGCATGCATTTCCCT+TGG	+	chr4_4:73838060-73838079	Msa0702270:three_prime_UTR	50.0%
!	AGTCCATTGACGGTGCCAAT+AGG	+	chr4_4:73838218-73838237	Msa0702270:three_prime_UTR	50.0%
	TCTCCTATTGGCACCGTCAA+TGG	-	chr4_4:73838224-73838243	None:intergenic	50.0%
	CTGGACAACGAATGAGATGC+AGG	-	chr4_4:73838264-73838283	None:intergenic	50.0%
	TTCGTTGTCCAGTGAAGAGC+AGG	+	chr4_4:73838272-73838291	Msa0702270:three_prime_UTR	50.0%
	TCCTGGTGCTCAGTCCTTTG+AGG	+	chr4_4:73836121-73836140	Msa0702270:intron	55.0%
	ACCTCAAAGGACTGAGCACC+AGG	-	chr4_4:73836125-73836144	None:intergenic	55.0%
	TGCAGCTGGAGCTGATAGTC+CGG	+	chr4_4:73836492-73836511	Msa0702270:CDS	55.0%
	TTTCAACGCAGCTCCTGCCA+TGG	+	chr4_4:73836860-73836879	Msa0702270:intron	55.0%
	GAGTGCTTTGCAAAGCACGC+AGG	+	chr4_4:73837022-73837041	Msa0702270:three_prime_UTR	55.0%
	GATACCGTGTTCTTCCGGGA+AGG	+	chr4_4:73837186-73837205	Msa0702270:three_prime_UTR	55.0%
	CGGTTTGCACCAGAACTTGG+TGG	+	chr4_4:73837296-73837315	Msa0702270:intron	55.0%
	TTGATCATGCCGGATGCGAG+TGG	+	chr4_4:73838102-73838121	Msa0702270:three_prime_UTR	55.0%
	CTCACATCTCCACTCGCATC+CGG	-	chr4_4:73838114-73838133	None:intergenic	55.0%
	GGAGTGATCGACACGCTCCA+AGG	+	chr4_4:73836082-73836101	Msa0702270:intron	60.0%
	CCTTTCCGACCTCTGGTGCT+TGG	-	chr4_4:73836565-73836584	None:intergenic	60.0%
	CCAAGCACCAGAGGTCGGAA+AGG	+	chr4_4:73836562-73836581	Msa0702270:CDS	60.0%
	CACAGAAGCCTGACCATGGC+AGG	-	chr4_4:73836876-73836895	None:intergenic	60.0%
	AGGGTCATGGTGTGCAGGGT+TGG	+	chr4_4:73838318-73838337	Msa0702270:three_prime_UTR	60.0%
	GGAGCGATCTACGCGCTCCA+AGG	+	chr4_4:73836283-73836302	Msa0702270:CDS	65.0%
	ACGCAGCTCCTGCCATGGTC+AGG	+	chr4_4:73836865-73836884	Msa0702270:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	73834575	73838607	73834575	ID=Msa0702270;Name=Msa0702270
chr4_4	mRNA	73834575	73838607	73834575	ID=Msa0702270-mRNA-1;Parent=Msa0702270;Name=Msa0702270-mRNA-1;_AED=0.19;_eAED=0.19;_QI=467\|0.83\|1\|1\|0.66\|0.57\|7\|1263\|108
chr4_4	exon	73834575	73835008	73834575	ID=Msa0702270-mRNA-1:exon:22771;Parent=Msa0702270-mRNA-1
chr4_4	exon	73835841	73835975	73835841	ID=Msa0702270-mRNA-1:exon:22772;Parent=Msa0702270-mRNA-1
chr4_4	exon	73836272	73836343	73836272	ID=Msa0702270-mRNA-1:exon:22773;Parent=Msa0702270-mRNA-1
chr4_4	exon	73836475	73836601	73836475	ID=Msa0702270-mRNA-1:exon:22774;Parent=Msa0702270-mRNA-1
chr4_4	exon	73836871	73837207	73836871	ID=Msa0702270-mRNA-1:exon:22775;Parent=Msa0702270-mRNA-1
chr4_4	exon	73837309	73837388	73837309	ID=Msa0702270-mRNA-1:exon:22776;Parent=Msa0702270-mRNA-1
chr4_4	exon	73837736	73838607	73837736	ID=Msa0702270-mRNA-1:exon:22777;Parent=Msa0702270-mRNA-1
chr4_4	five_prime_UTR	73834575	73835008	73834575	ID=Msa0702270-mRNA-1:five_prime_utr;Parent=Msa0702270-mRNA-1
chr4_4	five_prime_UTR	73835841	73835873	73835841	ID=Msa0702270-mRNA-1:five_prime_utr;Parent=Msa0702270-mRNA-1
chr4_4	CDS	73835874	73835975	73835874	ID=Msa0702270-mRNA-1:cds;Parent=Msa0702270-mRNA-1
chr4_4	CDS	73836272	73836343	73836272	ID=Msa0702270-mRNA-1:cds;Parent=Msa0702270-mRNA-1
chr4_4	CDS	73836475	73836601	73836475	ID=Msa0702270-mRNA-1:cds;Parent=Msa0702270-mRNA-1
chr4_4	CDS	73836871	73836896	73836871	ID=Msa0702270-mRNA-1:cds;Parent=Msa0702270-mRNA-1
chr4_4	three_prime_UTR	73836897	73837207	73836897	ID=Msa0702270-mRNA-1:three_prime_utr;Parent=Msa0702270-mRNA-1
chr4_4	three_prime_UTR	73837309	73837388	73837309	ID=Msa0702270-mRNA-1:three_prime_utr;Parent=Msa0702270-mRNA-1
chr4_4	three_prime_UTR	73837736	73838607	73837736	ID=Msa0702270-mRNA-1:three_prime_utr;Parent=Msa0702270-mRNA-1

Gene Sequence	>Msa0702270 ATGGGTTTAGAAGCTATTTTATTTCAGAAATCATTTTTCAAGCAATTTTATGGATCTGTGACAAAGTTGAACTATGCGACATTAAGGCTTGGTTTCATTAAGGAACGTGTTAAGGAGCGATCTACGCGCTCCAAGGAATCTTCACAATTCACTCTTGGTGCTCAGTCCTTTGAGGGTTTTCAAGGCAGAAGTGCAGCTGGAGCTGATAGTCCGGTTTCCTTCAGCGATGATGAAGATGCCATGCCAACATCAAGCTCAAAACCAAGCACCAGAGGTCGGAAAGGATCTGTTGCATCTAATTCTCCTGCCATGGTCAGGCTTCTGTGA
Protein sequence	>Msa0702270 MGLEAILFQKSFFKQFYGSVTKLNYATLRLGFIKERVKERSTRSKESSQFTLGAQSFEGFQGRSAAGADSPVSFSDDEDAMPTSSSKPSTRGRKGSVASNSPAMVRLL*