AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0336010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336010 MtrunA17_Chr2g0321571 100.000 107 0 0 1 107 1 107 6.46e-75 216
Msa0336010 MtrunA17_Chr4g0035271 99.065 107 1 0 1 107 1 107 2.02e-74 215
Msa0336010 MtrunA17_Chr6g0455051 75.556 45 2 1 26 70 6 41 1.05e-16 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336010 AT2G21580.1 91.667 108 8 1 1 107 1 108 2.42e-53 162
Msa0336010 AT4G39200.1 88.889 108 11 1 1 107 1 108 9.22e-52 158
Msa0336010 AT2G21580.2 87.963 108 11 2 1 107 1 107 1.09e-51 158
Msa0336010 AT4G39200.2 87.037 108 12 2 1 107 1 107 4.24e-50 154
Msa0336010 AT4G34555.1 88.785 107 11 1 1 106 1 107 9.74e-50 153
Msa0336010 AT2G16360.1 85.714 105 14 1 2 105 2 106 3.04e-46 144

Find 40 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAGTGATGAGTTTGTATT+TGG 0.207961 2_4:-55283275 None:intergenic
TGATTGCCGCAGAGAAGATT+TGG 0.323712 2_4:-55282971 None:intergenic
GAAAGCAAAAGAAGAAGAAA+TGG 0.344636 2_4:+55283170 Msa0336010:CDS
GGGGTTGGTGGTTACCATTA+GGG 0.358777 2_4:-55283010 None:intergenic
CAGTCCTCTCTGATCGTCTC+AGG 0.361688 2_4:+55283299 Msa0336010:CDS
CGCAGAGAAGATTTGGAAGA+AGG 0.369919 2_4:-55282964 None:intergenic
CATTATTATGAGGATGTGTT+TGG 0.370583 2_4:+55283566 Msa0336010:three_prime_UTR
TGATGGCAAGAGGATTGATT+AGG 0.387796 2_4:+55283443 Msa0336010:CDS
TCATCAAAGCCGGCGAAATC+AGG 0.410202 2_4:+55283142 Msa0336010:CDS
GGTGGTGGTGCTGCCTTCTT+CGG 0.414824 2_4:-55283118 None:intergenic
TCGTGCAATCAGAGATTTGA+TGG 0.416147 2_4:+55283426 Msa0336010:CDS
TCATCAACTCCAAACCCTAA+TGG 0.421313 2_4:+55282996 Msa0336010:exon
TGGTGGTTACCATTAGGGTT+TGG 0.424277 2_4:-55283005 None:intergenic
TGAAACCGCCTATGTATTAG+TGG 0.440250 2_4:-55283506 None:intergenic
CTAATACATAGGCGGTTTCA+AGG 0.473470 2_4:+55283509 Msa0336010:exon
AACATGGTCTTGTTTGATCA+AGG 0.477202 2_4:+55283223 Msa0336010:CDS
CAAAGGTAAACAAAAGGAAA+AGG 0.503611 2_4:+55283195 Msa0336010:CDS
TTCAGTGAGAAGCTTATCAT+AGG 0.513766 2_4:-55283248 None:intergenic
GATTTCGCCGGCTTTGATGA+CGG 0.516878 2_4:-55283139 None:intergenic
TTAGAGACAACATTATTATG+AGG 0.522671 2_4:+55283556 Msa0336010:three_prime_UTR
ATGGAGCAAAGGTAAACAAA+AGG 0.525529 2_4:+55283189 Msa0336010:CDS
ACCACCACCGTCATCAAAGC+CGG 0.527384 2_4:+55283132 Msa0336010:CDS
CACTAGAGCCACTAATACAT+AGG 0.531061 2_4:+55283498 Msa0336010:CDS
TTTGTTGTAGGCACCGAAGA+AGG 0.534954 2_4:+55283105 Msa0336010:intron
GGAGTGATGAGTTTGTATTT+GGG 0.540872 2_4:-55283274 None:intergenic
GCAGAGAAGATTTGGAAGAA+GGG 0.551616 2_4:-55282963 None:intergenic
ATCAGAGATTTGATGGCAAG+AGG 0.560553 2_4:+55283433 Msa0336010:CDS
TTTCCTCCTCCTGATTTCGC+CGG 0.569055 2_4:-55283151 None:intergenic
GCCGGCTTTGATGACGGTGG+TGG 0.569126 2_4:-55283133 None:intergenic
AGACGATCAGAGAGGACTGA+AGG 0.570313 2_4:-55283295 None:intergenic
GGCAAGAGGATTGATTAGGT+TGG 0.587429 2_4:+55283447 Msa0336010:CDS
AGTCCTCTCTGATCGTCTCA+GGG 0.592148 2_4:+55283300 Msa0336010:CDS
AAGAAGAAGAAATGGAGCAA+AGG 0.611863 2_4:+55283178 Msa0336010:CDS
AAGCCGGCGAAATCAGGAGG+AGG 0.614520 2_4:+55283148 Msa0336010:CDS
TCAAAGCCGGCGAAATCAGG+AGG 0.640955 2_4:+55283145 Msa0336010:CDS
AAAGGAAAAGGTTAACAACA+TGG 0.683005 2_4:+55283207 Msa0336010:CDS
CTTCTTCCAAATCTTCTCTG+CGG 0.690277 2_4:+55282965 Msa0336010:five_prime_UTR
TTCGCCGGCTTTGATGACGG+TGG 0.725481 2_4:-55283136 None:intergenic
TAGAGCCACTAATACATAGG+CGG 0.728751 2_4:+55283501 Msa0336010:exon
ATACCCTGAGACGATCAGAG+AGG 0.757534 2_4:-55283303 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGTTTACATTATTGTTTT+TGG + chr2_4:55283683-55283702 Msa0336010:three_prime_UTR 15.0%
!!! ATGTTTACATTATTGTTTTT+GGG + chr2_4:55283684-55283703 Msa0336010:three_prime_UTR 15.0%
!!! TTGTATAATTGATTTGTTTT+AGG + chr2_4:55283711-55283730 Msa0336010:three_prime_UTR 15.0%
!!! TATGTTTACATTATTGTTTT+TGG + chr2_4:55283683-55283702 Msa0336010:three_prime_UTR 15.0%
!!! ATGTTTACATTATTGTTTTT+GGG + chr2_4:55283684-55283703 Msa0336010:three_prime_UTR 15.0%
!!! TTGTATAATTGATTTGTTTT+AGG + chr2_4:55283711-55283730 Msa0336010:three_prime_UTR 15.0%
!!! TTGTAATGTTTTCAGATTAA+TGG + chr2_4:55283391-55283410 Msa0336010:intron 20.0%
!!! TTGATTTTACTCTAAAGTTT+AGG + chr2_4:55283746-55283765 Msa0336010:three_prime_UTR 20.0%
!!! TTGTAATGTTTTCAGATTAA+TGG + chr2_4:55283391-55283410 Msa0336010:intron 20.0%
!!! TTGATTTTACTCTAAAGTTT+AGG + chr2_4:55283746-55283765 Msa0336010:three_prime_UTR 20.0%
!!! TTTTCAGATTAATGGATCTT+TGG + chr2_4:55283399-55283418 Msa0336010:intron 25.0%
! TTAGAGACAACATTATTATG+AGG + chr2_4:55283556-55283575 Msa0336010:three_prime_UTR 25.0%
!!! TTTTCAGATTAATGGATCTT+TGG + chr2_4:55283399-55283418 Msa0336010:intron 25.0%
! TTAGAGACAACATTATTATG+AGG + chr2_4:55283556-55283575 Msa0336010:three_prime_UTR 25.0%
!! GAATTTGATGATGGAAATTG+GGG - chr2_4:55283032-55283051 None:intergenic 30.0%
!! GGAATTTGATGATGGAAATT+GGG - chr2_4:55283033-55283052 None:intergenic 30.0%
!! AGGAATTTGATGATGGAAAT+TGG - chr2_4:55283034-55283053 None:intergenic 30.0%
TAAGGAAAGGAATTTGATGA+TGG - chr2_4:55283041-55283060 None:intergenic 30.0%
!! ACTTCCATTTTCTTTGTTGT+AGG + chr2_4:55283093-55283112 Msa0336010:intron 30.0%
GAAAGCAAAAGAAGAAGAAA+TGG + chr2_4:55283170-55283189 Msa0336010:CDS 30.0%
CAAAGGTAAACAAAAGGAAA+AGG + chr2_4:55283195-55283214 Msa0336010:CDS 30.0%
AAAGGAAAAGGTTAACAACA+TGG + chr2_4:55283207-55283226 Msa0336010:CDS 30.0%
CATTATTATGAGGATGTGTT+TGG + chr2_4:55283566-55283585 Msa0336010:three_prime_UTR 30.0%
!!! TTTTACTCTAAAGTTTAGGC+AGG + chr2_4:55283750-55283769 Msa0336010:three_prime_UTR 30.0%
!! TTTACTCTAAAGTTTAGGCA+GGG + chr2_4:55283751-55283770 Msa0336010:three_prime_UTR 30.0%
!! GAATTTGATGATGGAAATTG+GGG - chr2_4:55283032-55283051 None:intergenic 30.0%
!! GGAATTTGATGATGGAAATT+GGG - chr2_4:55283033-55283052 None:intergenic 30.0%
!! AGGAATTTGATGATGGAAAT+TGG - chr2_4:55283034-55283053 None:intergenic 30.0%
TAAGGAAAGGAATTTGATGA+TGG - chr2_4:55283041-55283060 None:intergenic 30.0%
!! ACTTCCATTTTCTTTGTTGT+AGG + chr2_4:55283093-55283112 Msa0336010:intron 30.0%
GAAAGCAAAAGAAGAAGAAA+TGG + chr2_4:55283170-55283189 Msa0336010:CDS 30.0%
CAAAGGTAAACAAAAGGAAA+AGG + chr2_4:55283195-55283214 Msa0336010:CDS 30.0%
AAAGGAAAAGGTTAACAACA+TGG + chr2_4:55283207-55283226 Msa0336010:CDS 30.0%
CATTATTATGAGGATGTGTT+TGG + chr2_4:55283566-55283585 Msa0336010:three_prime_UTR 30.0%
CGAGATTTAGATCTAAGGAA+AGG - chr2_4:55283054-55283073 None:intergenic 35.0%
TGCAACGAGATTTAGATCTA+AGG - chr2_4:55283059-55283078 None:intergenic 35.0%
AAGAAGAAGAAATGGAGCAA+AGG + chr2_4:55283178-55283197 Msa0336010:CDS 35.0%
ATGGAGCAAAGGTAAACAAA+AGG + chr2_4:55283189-55283208 Msa0336010:CDS 35.0%
!! AACATGGTCTTGTTTGATCA+AGG + chr2_4:55283223-55283242 Msa0336010:CDS 35.0%
TTCAGTGAGAAGCTTATCAT+AGG - chr2_4:55283251-55283270 None:intergenic 35.0%
GGAGTGATGAGTTTGTATTT+GGG - chr2_4:55283277-55283296 None:intergenic 35.0%
AGGAGTGATGAGTTTGTATT+TGG - chr2_4:55283278-55283297 None:intergenic 35.0%
CGAGATTTAGATCTAAGGAA+AGG - chr2_4:55283054-55283073 None:intergenic 35.0%
TGCAACGAGATTTAGATCTA+AGG - chr2_4:55283059-55283078 None:intergenic 35.0%
AAGAAGAAGAAATGGAGCAA+AGG + chr2_4:55283178-55283197 Msa0336010:CDS 35.0%
ATGGAGCAAAGGTAAACAAA+AGG + chr2_4:55283189-55283208 Msa0336010:CDS 35.0%
!! AACATGGTCTTGTTTGATCA+AGG + chr2_4:55283223-55283242 Msa0336010:CDS 35.0%
TTCAGTGAGAAGCTTATCAT+AGG - chr2_4:55283251-55283270 None:intergenic 35.0%
GGAGTGATGAGTTTGTATTT+GGG - chr2_4:55283277-55283296 None:intergenic 35.0%
AGGAGTGATGAGTTTGTATT+TGG - chr2_4:55283278-55283297 None:intergenic 35.0%
GCAGAGAAGATTTGGAAGAA+GGG - chr2_4:55282966-55282985 None:intergenic 40.0%
CTTCTTCCAAATCTTCTCTG+CGG + chr2_4:55282965-55282984 Msa0336010:five_prime_UTR 40.0%
TCATCAACTCCAAACCCTAA+TGG + chr2_4:55282996-55283015 Msa0336010:exon 40.0%
!! TTGATGATGGAAATTGGGGT+TGG - chr2_4:55283028-55283047 None:intergenic 40.0%
GGTGCCTACAACAAAGAAAA+TGG - chr2_4:55283100-55283119 None:intergenic 40.0%
GAATTGAATCGAAGCGACAA+AGG - chr2_4:55283354-55283373 None:intergenic 40.0%
TCGTGCAATCAGAGATTTGA+TGG + chr2_4:55283426-55283445 Msa0336010:CDS 40.0%
! ATCAGAGATTTGATGGCAAG+AGG + chr2_4:55283433-55283452 Msa0336010:CDS 40.0%
TGATGGCAAGAGGATTGATT+AGG + chr2_4:55283443-55283462 Msa0336010:CDS 40.0%
CACTAGAGCCACTAATACAT+AGG + chr2_4:55283498-55283517 Msa0336010:CDS 40.0%
TAGAGCCACTAATACATAGG+CGG + chr2_4:55283501-55283520 Msa0336010:exon 40.0%
TGAAACCGCCTATGTATTAG+TGG - chr2_4:55283509-55283528 None:intergenic 40.0%
CTAATACATAGGCGGTTTCA+AGG + chr2_4:55283509-55283528 Msa0336010:exon 40.0%
GCAGAGAAGATTTGGAAGAA+GGG - chr2_4:55282966-55282985 None:intergenic 40.0%
CTTCTTCCAAATCTTCTCTG+CGG + chr2_4:55282965-55282984 Msa0336010:five_prime_UTR 40.0%
TCATCAACTCCAAACCCTAA+TGG + chr2_4:55282996-55283015 Msa0336010:exon 40.0%
!! TTGATGATGGAAATTGGGGT+TGG - chr2_4:55283028-55283047 None:intergenic 40.0%
GGTGCCTACAACAAAGAAAA+TGG - chr2_4:55283100-55283119 None:intergenic 40.0%
GAATTGAATCGAAGCGACAA+AGG - chr2_4:55283354-55283373 None:intergenic 40.0%
TCGTGCAATCAGAGATTTGA+TGG + chr2_4:55283426-55283445 Msa0336010:CDS 40.0%
! ATCAGAGATTTGATGGCAAG+AGG + chr2_4:55283433-55283452 Msa0336010:CDS 40.0%
TGATGGCAAGAGGATTGATT+AGG + chr2_4:55283443-55283462 Msa0336010:CDS 40.0%
CACTAGAGCCACTAATACAT+AGG + chr2_4:55283498-55283517 Msa0336010:CDS 40.0%
TAGAGCCACTAATACATAGG+CGG + chr2_4:55283501-55283520 Msa0336010:exon 40.0%
TGAAACCGCCTATGTATTAG+TGG - chr2_4:55283509-55283528 None:intergenic 40.0%
CTAATACATAGGCGGTTTCA+AGG + chr2_4:55283509-55283528 Msa0336010:exon 40.0%
CGCAGAGAAGATTTGGAAGA+AGG - chr2_4:55282967-55282986 None:intergenic 45.0%
TGATTGCCGCAGAGAAGATT+TGG - chr2_4:55282974-55282993 None:intergenic 45.0%
TGGTGGTTACCATTAGGGTT+TGG - chr2_4:55283008-55283027 None:intergenic 45.0%
! ATGATGGAAATTGGGGTTGG+TGG - chr2_4:55283025-55283044 None:intergenic 45.0%
!! TTTGTTGTAGGCACCGAAGA+AGG + chr2_4:55283105-55283124 Msa0336010:intron 45.0%
GGCAAGAGGATTGATTAGGT+TGG + chr2_4:55283447-55283466 Msa0336010:CDS 45.0%
CGCAGAGAAGATTTGGAAGA+AGG - chr2_4:55282967-55282986 None:intergenic 45.0%
TGATTGCCGCAGAGAAGATT+TGG - chr2_4:55282974-55282993 None:intergenic 45.0%
TGGTGGTTACCATTAGGGTT+TGG - chr2_4:55283008-55283027 None:intergenic 45.0%
! ATGATGGAAATTGGGGTTGG+TGG - chr2_4:55283025-55283044 None:intergenic 45.0%
!! TTTGTTGTAGGCACCGAAGA+AGG + chr2_4:55283105-55283124 Msa0336010:intron 45.0%
GGCAAGAGGATTGATTAGGT+TGG + chr2_4:55283447-55283466 Msa0336010:CDS 45.0%
!! GGGGTTGGTGGTTACCATTA+GGG - chr2_4:55283013-55283032 None:intergenic 50.0%
!! TGGGGTTGGTGGTTACCATT+AGG - chr2_4:55283014-55283033 None:intergenic 50.0%
!! GATTTCGCCGGCTTTGATGA+CGG - chr2_4:55283142-55283161 None:intergenic 50.0%
TCATCAAAGCCGGCGAAATC+AGG + chr2_4:55283142-55283161 Msa0336010:CDS 50.0%
TTTCCTCCTCCTGATTTCGC+CGG - chr2_4:55283154-55283173 None:intergenic 50.0%
AGACGATCAGAGAGGACTGA+AGG - chr2_4:55283298-55283317 None:intergenic 50.0%
AGTCCTCTCTGATCGTCTCA+GGG + chr2_4:55283300-55283319 Msa0336010:CDS 50.0%
ATACCCTGAGACGATCAGAG+AGG - chr2_4:55283306-55283325 None:intergenic 50.0%
!! GGGGTTGGTGGTTACCATTA+GGG - chr2_4:55283013-55283032 None:intergenic 50.0%
!! TGGGGTTGGTGGTTACCATT+AGG - chr2_4:55283014-55283033 None:intergenic 50.0%
!! GATTTCGCCGGCTTTGATGA+CGG - chr2_4:55283142-55283161 None:intergenic 50.0%
TCATCAAAGCCGGCGAAATC+AGG + chr2_4:55283142-55283161 Msa0336010:CDS 50.0%
TTTCCTCCTCCTGATTTCGC+CGG - chr2_4:55283154-55283173 None:intergenic 50.0%
AGACGATCAGAGAGGACTGA+AGG - chr2_4:55283298-55283317 None:intergenic 50.0%
AGTCCTCTCTGATCGTCTCA+GGG + chr2_4:55283300-55283319 Msa0336010:CDS 50.0%
ATACCCTGAGACGATCAGAG+AGG - chr2_4:55283306-55283325 None:intergenic 50.0%
ACCACCACCGTCATCAAAGC+CGG + chr2_4:55283132-55283151 Msa0336010:CDS 55.0%
TCAAAGCCGGCGAAATCAGG+AGG + chr2_4:55283145-55283164 Msa0336010:CDS 55.0%
CAGTCCTCTCTGATCGTCTC+AGG + chr2_4:55283299-55283318 Msa0336010:CDS 55.0%
ACCACCACCGTCATCAAAGC+CGG + chr2_4:55283132-55283151 Msa0336010:CDS 55.0%
TCAAAGCCGGCGAAATCAGG+AGG + chr2_4:55283145-55283164 Msa0336010:CDS 55.0%
CAGTCCTCTCTGATCGTCTC+AGG + chr2_4:55283299-55283318 Msa0336010:CDS 55.0%
!! GGTGGTGGTGCTGCCTTCTT+CGG - chr2_4:55283121-55283140 None:intergenic 60.0%
!! TTCGCCGGCTTTGATGACGG+TGG - chr2_4:55283139-55283158 None:intergenic 60.0%
AAGCCGGCGAAATCAGGAGG+AGG + chr2_4:55283148-55283167 Msa0336010:CDS 60.0%
!! GGTGGTGGTGCTGCCTTCTT+CGG - chr2_4:55283121-55283140 None:intergenic 60.0%
!! TTCGCCGGCTTTGATGACGG+TGG - chr2_4:55283139-55283158 None:intergenic 60.0%
AAGCCGGCGAAATCAGGAGG+AGG + chr2_4:55283148-55283167 Msa0336010:CDS 60.0%
!! GCCGGCTTTGATGACGGTGG+TGG - chr2_4:55283136-55283155 None:intergenic 65.0%
!! GCCGGCTTTGATGACGGTGG+TGG - chr2_4:55283136-55283155 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 55282958 55283772 55282958 ID=Msa0336010;Name=Msa0336010
chr2_4 mRNA 55282958 55283772 55282958 ID=Msa0336010-mRNA-1;Parent=Msa0336010;Name=Msa0336010-mRNA-1;_AED=0.06;_eAED=0.06;_QI=57|1|1|1|1|1|3|253|107
chr2_4 exon 55282958 55283017 55282958 ID=Msa0336010-mRNA-1:exon:16725;Parent=Msa0336010-mRNA-1
chr2_4 exon 55283115 55283321 55283115 ID=Msa0336010-mRNA-1:exon:16726;Parent=Msa0336010-mRNA-1
chr2_4 exon 55283406 55283772 55283406 ID=Msa0336010-mRNA-1:exon:16727;Parent=Msa0336010-mRNA-1
chr2_4 five_prime_UTR 55282958 55283014 55282958 ID=Msa0336010-mRNA-1:five_prime_utr;Parent=Msa0336010-mRNA-1
chr2_4 CDS 55283015 55283017 55283015 ID=Msa0336010-mRNA-1:cds;Parent=Msa0336010-mRNA-1
chr2_4 CDS 55283115 55283321 55283115 ID=Msa0336010-mRNA-1:cds;Parent=Msa0336010-mRNA-1
chr2_4 CDS 55283406 55283519 55283406 ID=Msa0336010-mRNA-1:cds;Parent=Msa0336010-mRNA-1
chr2_4 three_prime_UTR 55283520 55283772 55283520 ID=Msa0336010-mRNA-1:three_prime_utr;Parent=Msa0336010-mRNA-1
Gene Sequence

>Msa0336010

ATGGCACCGAAGAAGGCAGCACCACCACCGTCATCAAAGCCGGCGAAATCAGGAGGAGGAAAGCAAAAGAAGAAGAAATGGAGCAAAGGTAAACAAAAGGAAAAGGTTAACAACATGGTCTTGTTTGATCAAGGTACCTATGATAAGCTTCTCACTGAAGCTCCCAAATACAAACTCATCACTCCTTCAGTCCTCTCTGATCGTCTCAGGATTAATGGATCTTTGGCACGTCGTGCAATCAGAGATTTGATGGCAAGAGGATTGATTAGGTTGGTGTCTGCTCATTCTAGTCAGCAGATTTACACTAGAGCCACTAATACATAG

Protein sequence

>Msa0336010

MAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMARGLIRLVSAHSSQQIYTRATNT*