AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0546880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546880 MtrunA17_Chr4g0005151 92.982 114 8 0 1 114 1 114 6.61e-75 217
Msa0546880 MtrunA17_Chr7g0243451 80.392 102 20 0 1 102 1 102 6.38e-58 174
Msa0546880 MtrunA17_Chr4g0005161 56.863 102 44 0 1 102 1 102 2.16e-39 127
Msa0546880 MtrunA17_Chr7g0238011 44.660 103 57 0 1 103 1 103 1.22e-29 103
Msa0546880 MtrunA17_Chr4g0071771 46.078 102 55 0 1 102 1 102 2.73e-26 94.4
Msa0546880 MtrunA17_Chr4g0071761 41.748 103 60 0 1 103 1 103 1.02e-23 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546880 AT3G51660.1 59.223 103 42 0 1 103 1 103 1.60e-41 133
Msa0546880 AT5G01650.2 55.882 102 45 0 1 102 1 102 4.26e-40 130
Msa0546880 AT5G01650.3 55.882 102 45 0 1 102 1 102 4.26e-40 130
Msa0546880 AT5G01650.4 55.882 102 45 0 1 102 1 102 7.40e-40 129
Msa0546880 AT5G01650.1 55.882 102 45 0 1 102 1 102 1.12e-39 128
Msa0546880 AT5G57170.2 46.078 102 55 0 1 102 1 102 2.61e-26 94.7
Msa0546880 AT5G57170.1 46.078 102 55 0 1 102 1 102 5.63e-26 94.0

Find 23 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACCAACGTTAATTTAGA+TGG 0.290910 4_1:-39364354 Msa0546880:CDS
CAGTACCATTGGCACAATAT+TGG 0.301904 4_1:-39362868 Msa0546880:CDS
ACTGCTGTTTCCACAATCAT+AGG 0.405897 4_1:-39364297 Msa0546880:CDS
TCAAAAGCATCACTACAGTT+TGG 0.409372 4_1:+39362767 None:intergenic
TCTCAAAGTCTTTGACATCA+TGG 0.431478 4_1:-39362811 Msa0546880:CDS
GAAGAGTGACATACCTTGTC+GGG 0.442658 4_1:+39364270 None:intergenic
GTTCTAGCTTGATCAAAGAT+AGG 0.446427 4_1:+39364318 None:intergenic
GTTTGTGATGGTTCTATTGA+AGG 0.461686 4_1:-39362991 Msa0546880:CDS
TGTTCTTGGTATAGAGAGGT+TGG 0.461721 4_1:+39362842 None:intergenic
AACAAAGAACCAGCTGCATA+TGG 0.465591 4_1:-39362942 Msa0546880:CDS
TTTGTGATGGTTCTATTGAA+GGG 0.504158 4_1:-39362990 Msa0546880:CDS
TAACGTTGGTAGAGATGTAA+AGG 0.513197 4_1:+39364364 None:intergenic
ATATGGTGAACTAGTTTCAA+TGG 0.521191 4_1:-39362925 Msa0546880:CDS
AAGAATCTAATCAGTACCAT+TGG 0.529298 4_1:-39362879 Msa0546880:CDS
GGATTCCAATATTGTGCCAA+TGG 0.539625 4_1:+39362863 None:intergenic
ATCTTGTTCTTGGTATAGAG+AGG 0.571975 4_1:+39362838 None:intergenic
ACTAGTTCACCATATGCAGC+TGG 0.580662 4_1:+39362933 None:intergenic
TTGTGATGGTTCTATTGAAG+GGG 0.584227 4_1:-39362989 Msa0546880:CDS
TATGGTGAACTAGTTTCAAT+GGG 0.596371 4_1:-39362924 Msa0546880:CDS
AGAAGAGTGACATACCTTGT+CGG 0.618285 4_1:+39364269 None:intergenic
GGTGAACTAGTTTCAATGGG+TGG 0.635436 4_1:-39362921 Msa0546880:CDS
TTGGTAGAGATGTAAAGGCA+AGG 0.650566 4_1:+39364369 None:intergenic
AATCATAGGAAAACCCGACA+AGG 0.692062 4_1:-39364283 Msa0546880:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAAACTAATTTCAAT+GGG - chr4_1:39363386-39363405 Msa0546880:intron 10.0%
!! TTAAATAAACTAATTTCAAT+GGG - chr4_1:39363386-39363405 Msa0546880:intron 10.0%
!!! CACAAAATTAATATGAATTT+TGG - chr4_1:39362977-39362996 Msa0546880:CDS 15.0%
!!! ATTTATTTCTCAAAATCAAA+TGG - chr4_1:39363287-39363306 Msa0546880:intron 15.0%
!! GTTAAATAAACTAATTTCAA+TGG - chr4_1:39363385-39363404 Msa0546880:intron 15.0%
!! AAATAAAAAAGAATTTCACT+TGG + chr4_1:39363721-39363740 None:intergenic 15.0%
!!! CACAAAATTAATATGAATTT+TGG - chr4_1:39362977-39362996 Msa0546880:CDS 15.0%
!!! ATTTATTTCTCAAAATCAAA+TGG - chr4_1:39363287-39363306 Msa0546880:intron 15.0%
!! GTTAAATAAACTAATTTCAA+TGG - chr4_1:39363385-39363404 Msa0546880:intron 15.0%
!! AAATAAAAAAGAATTTCACT+TGG + chr4_1:39363721-39363740 None:intergenic 15.0%
!!! ATTTTGGATTTTGTTGATTA+TGG - chr4_1:39362993-39363012 Msa0546880:CDS 20.0%
!!! TTGTTATTTTCAAACGATAT+TGG + chr4_1:39364194-39364213 None:intergenic 20.0%
!!! ATTTTGGATTTTGTTGATTA+TGG - chr4_1:39362993-39363012 Msa0546880:CDS 20.0%
!!! TTGTTATTTTCAAACGATAT+TGG + chr4_1:39364194-39364213 None:intergenic 20.0%
!! TTTTCCATCTAAATTAACGT+TGG + chr4_1:39362804-39362823 None:intergenic 25.0%
!! TGTTTTACAAATAAAGCTTG+TGG - chr4_1:39362914-39362933 Msa0546880:CDS 25.0%
!! ACTGATTCATAATGTTTGAT+TGG - chr4_1:39363062-39363081 Msa0546880:intron 25.0%
! AAGTAAGTAAATAGCCAAAT+TGG - chr4_1:39363832-39363851 Msa0546880:intron 25.0%
! CCTCTAATGATAAAAATTGA+TGG + chr4_1:39363951-39363970 None:intergenic 25.0%
!! CCATCAATTTTTATCATTAG+AGG - chr4_1:39363948-39363967 Msa0546880:intron 25.0%
! ACATACATAGTGATATTTCA+AGG - chr4_1:39363981-39364000 Msa0546880:intron 25.0%
!!! ATACCATGCAATTTAGTTTT+GGG + chr4_1:39364032-39364051 None:intergenic 25.0%
!!! TATACCATGCAATTTAGTTT+TGG + chr4_1:39364033-39364052 None:intergenic 25.0%
! GCATGGTATAAAAATTTATG+AGG - chr4_1:39364043-39364062 Msa0546880:intron 25.0%
! TTGAGAAAAAATCTTGTTCT+TGG + chr4_1:39364326-39364345 None:intergenic 25.0%
!! TTTTCCATCTAAATTAACGT+TGG + chr4_1:39362804-39362823 None:intergenic 25.0%
!! TGTTTTACAAATAAAGCTTG+TGG - chr4_1:39362914-39362933 Msa0546880:CDS 25.0%
!! ACTGATTCATAATGTTTGAT+TGG - chr4_1:39363062-39363081 Msa0546880:intron 25.0%
! AAGTAAGTAAATAGCCAAAT+TGG - chr4_1:39363832-39363851 Msa0546880:intron 25.0%
! CCTCTAATGATAAAAATTGA+TGG + chr4_1:39363951-39363970 None:intergenic 25.0%
!! CCATCAATTTTTATCATTAG+AGG - chr4_1:39363948-39363967 Msa0546880:intron 25.0%
! ACATACATAGTGATATTTCA+AGG - chr4_1:39363981-39364000 Msa0546880:intron 25.0%
!!! ATACCATGCAATTTAGTTTT+GGG + chr4_1:39364032-39364051 None:intergenic 25.0%
!!! TATACCATGCAATTTAGTTT+TGG + chr4_1:39364033-39364052 None:intergenic 25.0%
! GCATGGTATAAAAATTTATG+AGG - chr4_1:39364043-39364062 Msa0546880:intron 25.0%
! TTGAGAAAAAATCTTGTTCT+TGG + chr4_1:39364326-39364345 None:intergenic 25.0%
TCTACCAACGTTAATTTAGA+TGG - chr4_1:39362797-39362816 Msa0546880:CDS 30.0%
CAAATAAAGCTTGTGGAATT+TGG - chr4_1:39362921-39362940 Msa0546880:CDS 30.0%
GTTGTGTGAAAATCCTTTAA+GGG - chr4_1:39362950-39362969 Msa0546880:CDS 30.0%
! AATTTTGTGTCAACCCTTAA+AGG + chr4_1:39362966-39362985 None:intergenic 30.0%
!! CATAATGTTTGATTGGTTTC+TGG - chr4_1:39363069-39363088 Msa0546880:intron 30.0%
!! TGATTGGTTTCTGGTATAAT+TGG - chr4_1:39363078-39363097 Msa0546880:intron 30.0%
! TGACAAGTTTTCTACAGAAA+AGG + chr4_1:39363217-39363236 None:intergenic 30.0%
! TGCTCTTTGATGCAATTTTT+AGG - chr4_1:39363494-39363513 Msa0546880:intron 30.0%
! GCTCTTTGATGCAATTTTTA+GGG - chr4_1:39363495-39363514 Msa0546880:intron 30.0%
!!! CAATTTAGTTTTGGGTTATG+TGG + chr4_1:39364024-39364043 None:intergenic 30.0%
TAACCCAAAACTAAATTGCA+TGG - chr4_1:39364026-39364045 Msa0546880:intron 30.0%
! GTAATTTTTGCAGTTTGTGA+TGG - chr4_1:39364148-39364167 Msa0546880:intron 30.0%
! TTTGTGATGGTTCTATTGAA+GGG - chr4_1:39364161-39364180 Msa0546880:intron 30.0%
! ATATGGTGAACTAGTTTCAA+TGG - chr4_1:39364226-39364245 Msa0546880:intron 30.0%
! TATGGTGAACTAGTTTCAAT+GGG - chr4_1:39364227-39364246 Msa0546880:intron 30.0%
AAGAATCTAATCAGTACCAT+TGG - chr4_1:39364272-39364291 Msa0546880:intron 30.0%
TCTACCAACGTTAATTTAGA+TGG - chr4_1:39362797-39362816 Msa0546880:CDS 30.0%
CAAATAAAGCTTGTGGAATT+TGG - chr4_1:39362921-39362940 Msa0546880:CDS 30.0%
GTTGTGTGAAAATCCTTTAA+GGG - chr4_1:39362950-39362969 Msa0546880:CDS 30.0%
! AATTTTGTGTCAACCCTTAA+AGG + chr4_1:39362966-39362985 None:intergenic 30.0%
!! CATAATGTTTGATTGGTTTC+TGG - chr4_1:39363069-39363088 Msa0546880:intron 30.0%
!! TGATTGGTTTCTGGTATAAT+TGG - chr4_1:39363078-39363097 Msa0546880:intron 30.0%
! TGACAAGTTTTCTACAGAAA+AGG + chr4_1:39363217-39363236 None:intergenic 30.0%
! TGCTCTTTGATGCAATTTTT+AGG - chr4_1:39363494-39363513 Msa0546880:intron 30.0%
! GCTCTTTGATGCAATTTTTA+GGG - chr4_1:39363495-39363514 Msa0546880:intron 30.0%
!!! CAATTTAGTTTTGGGTTATG+TGG + chr4_1:39364024-39364043 None:intergenic 30.0%
TAACCCAAAACTAAATTGCA+TGG - chr4_1:39364026-39364045 Msa0546880:intron 30.0%
! GTAATTTTTGCAGTTTGTGA+TGG - chr4_1:39364148-39364167 Msa0546880:intron 30.0%
! TTTGTGATGGTTCTATTGAA+GGG - chr4_1:39364161-39364180 Msa0546880:intron 30.0%
! ATATGGTGAACTAGTTTCAA+TGG - chr4_1:39364226-39364245 Msa0546880:intron 30.0%
! TATGGTGAACTAGTTTCAAT+GGG - chr4_1:39364227-39364246 Msa0546880:intron 30.0%
AAGAATCTAATCAGTACCAT+TGG - chr4_1:39364272-39364291 Msa0546880:intron 30.0%
! TAACGTTGGTAGAGATGTAA+AGG + chr4_1:39362790-39362809 None:intergenic 35.0%
!! GTTCTAGCTTGATCAAAGAT+AGG + chr4_1:39362836-39362855 None:intergenic 35.0%
! AGCTTGTGGAATTTGGTTTA+AGG - chr4_1:39362928-39362947 Msa0546880:CDS 35.0%
GGTTGTGTGAAAATCCTTTA+AGG - chr4_1:39362949-39362968 Msa0546880:CDS 35.0%
AGAAAAGGCAGAGAGTAAAA+GGG + chr4_1:39363202-39363221 None:intergenic 35.0%
AAATAAGACAGCAGACTAGT+TGG - chr4_1:39363433-39363452 Msa0546880:intron 35.0%
CTTGGAACAAAGTACAACAT+GGG + chr4_1:39363703-39363722 None:intergenic 35.0%
ACTTGGAACAAAGTACAACA+TGG + chr4_1:39363704-39363723 None:intergenic 35.0%
TCTTTGTGCGCAAAGTTAAT+AGG + chr4_1:39363803-39363822 None:intergenic 35.0%
! TTTGCGCACAAAGAATTTTG+CGG - chr4_1:39363809-39363828 Msa0546880:intron 35.0%
!!! CACAGTTTTGAGTACCAATT+TGG + chr4_1:39363849-39363868 None:intergenic 35.0%
! GTTTGTGATGGTTCTATTGA+AGG - chr4_1:39364160-39364179 Msa0546880:intron 35.0%
! TTGTGATGGTTCTATTGAAG+GGG - chr4_1:39364162-39364181 Msa0546880:intron 35.0%
ATCTTGTTCTTGGTATAGAG+AGG + chr4_1:39364316-39364335 None:intergenic 35.0%
TCTCAAAGTCTTTGACATCA+TGG - chr4_1:39364340-39364359 Msa0546880:CDS 35.0%
! TAACGTTGGTAGAGATGTAA+AGG + chr4_1:39362790-39362809 None:intergenic 35.0%
!! GTTCTAGCTTGATCAAAGAT+AGG + chr4_1:39362836-39362855 None:intergenic 35.0%
! AGCTTGTGGAATTTGGTTTA+AGG - chr4_1:39362928-39362947 Msa0546880:CDS 35.0%
GGTTGTGTGAAAATCCTTTA+AGG - chr4_1:39362949-39362968 Msa0546880:CDS 35.0%
AGAAAAGGCAGAGAGTAAAA+GGG + chr4_1:39363202-39363221 None:intergenic 35.0%
AAATAAGACAGCAGACTAGT+TGG - chr4_1:39363433-39363452 Msa0546880:intron 35.0%
CTTGGAACAAAGTACAACAT+GGG + chr4_1:39363703-39363722 None:intergenic 35.0%
ACTTGGAACAAAGTACAACA+TGG + chr4_1:39363704-39363723 None:intergenic 35.0%
TCTTTGTGCGCAAAGTTAAT+AGG + chr4_1:39363803-39363822 None:intergenic 35.0%
! TTTGCGCACAAAGAATTTTG+CGG - chr4_1:39363809-39363828 Msa0546880:intron 35.0%
!!! CACAGTTTTGAGTACCAATT+TGG + chr4_1:39363849-39363868 None:intergenic 35.0%
! GTTTGTGATGGTTCTATTGA+AGG - chr4_1:39364160-39364179 Msa0546880:intron 35.0%
! TTGTGATGGTTCTATTGAAG+GGG - chr4_1:39364162-39364181 Msa0546880:intron 35.0%
ATCTTGTTCTTGGTATAGAG+AGG + chr4_1:39364316-39364335 None:intergenic 35.0%
TCTCAAAGTCTTTGACATCA+TGG - chr4_1:39364340-39364359 Msa0546880:CDS 35.0%
TTGGTAGAGATGTAAAGGCA+AGG + chr4_1:39362785-39362804 None:intergenic 40.0%
!! ACTGCTGTTTCCACAATCAT+AGG - chr4_1:39362854-39362873 Msa0546880:CDS 40.0%
AATCATAGGAAAACCCGACA+AGG - chr4_1:39362868-39362887 Msa0546880:CDS 40.0%
AGAAGAGTGACATACCTTGT+CGG + chr4_1:39362885-39362904 None:intergenic 40.0%
GAAGTGTACACTTCTCTGTT+TGG - chr4_1:39363147-39363166 Msa0546880:intron 40.0%
CAGAAAAGGCAGAGAGTAAA+AGG + chr4_1:39363203-39363222 None:intergenic 40.0%
GATAACTTCTATGTGCTGGA+CGG + chr4_1:39363758-39363777 None:intergenic 40.0%
CCATGATAACTTCTATGTGC+TGG + chr4_1:39363762-39363781 None:intergenic 40.0%
CCAGCACATAGAAGTTATCA+TGG - chr4_1:39363759-39363778 Msa0546880:intron 40.0%
! CGATGTGATAGTCTTAGAGT+TGG - chr4_1:39364093-39364112 Msa0546880:intron 40.0%
AACAAAGAACCAGCTGCATA+TGG - chr4_1:39364209-39364228 Msa0546880:intron 40.0%
CAGTACCATTGGCACAATAT+TGG - chr4_1:39364283-39364302 Msa0546880:intron 40.0%
GGATTCCAATATTGTGCCAA+TGG + chr4_1:39364291-39364310 None:intergenic 40.0%
TGTTCTTGGTATAGAGAGGT+TGG + chr4_1:39364312-39364331 None:intergenic 40.0%
TTGGTAGAGATGTAAAGGCA+AGG + chr4_1:39362785-39362804 None:intergenic 40.0%
!! ACTGCTGTTTCCACAATCAT+AGG - chr4_1:39362854-39362873 Msa0546880:CDS 40.0%
AATCATAGGAAAACCCGACA+AGG - chr4_1:39362868-39362887 Msa0546880:CDS 40.0%
AGAAGAGTGACATACCTTGT+CGG + chr4_1:39362885-39362904 None:intergenic 40.0%
GAAGTGTACACTTCTCTGTT+TGG - chr4_1:39363147-39363166 Msa0546880:intron 40.0%
CAGAAAAGGCAGAGAGTAAA+AGG + chr4_1:39363203-39363222 None:intergenic 40.0%
GATAACTTCTATGTGCTGGA+CGG + chr4_1:39363758-39363777 None:intergenic 40.0%
CCATGATAACTTCTATGTGC+TGG + chr4_1:39363762-39363781 None:intergenic 40.0%
CCAGCACATAGAAGTTATCA+TGG - chr4_1:39363759-39363778 Msa0546880:intron 40.0%
! CGATGTGATAGTCTTAGAGT+TGG - chr4_1:39364093-39364112 Msa0546880:intron 40.0%
AACAAAGAACCAGCTGCATA+TGG - chr4_1:39364209-39364228 Msa0546880:intron 40.0%
CAGTACCATTGGCACAATAT+TGG - chr4_1:39364283-39364302 Msa0546880:intron 40.0%
GGATTCCAATATTGTGCCAA+TGG + chr4_1:39364291-39364310 None:intergenic 40.0%
TGTTCTTGGTATAGAGAGGT+TGG + chr4_1:39364312-39364331 None:intergenic 40.0%
! GTCGGGTTTTCCTATGATTG+TGG + chr4_1:39362867-39362886 None:intergenic 45.0%
GAAGAGTGACATACCTTGTC+GGG + chr4_1:39362884-39362903 None:intergenic 45.0%
! TAAGACAGCAGACTAGTTGG+AGG - chr4_1:39363436-39363455 Msa0546880:intron 45.0%
! TTGGAGGCTTTTCTGTCTGT+AGG - chr4_1:39363452-39363471 Msa0546880:intron 45.0%
GGGTTATGTGGCATTGTTGT+AGG + chr4_1:39364012-39364031 None:intergenic 45.0%
ACTAGTTCACCATATGCAGC+TGG + chr4_1:39364221-39364240 None:intergenic 45.0%
! GGTGAACTAGTTTCAATGGG+TGG - chr4_1:39364230-39364249 Msa0546880:intron 45.0%
! GTCGGGTTTTCCTATGATTG+TGG + chr4_1:39362867-39362886 None:intergenic 45.0%
GAAGAGTGACATACCTTGTC+GGG + chr4_1:39362884-39362903 None:intergenic 45.0%
! TAAGACAGCAGACTAGTTGG+AGG - chr4_1:39363436-39363455 Msa0546880:intron 45.0%
! TTGGAGGCTTTTCTGTCTGT+AGG - chr4_1:39363452-39363471 Msa0546880:intron 45.0%
GGGTTATGTGGCATTGTTGT+AGG + chr4_1:39364012-39364031 None:intergenic 45.0%
ACTAGTTCACCATATGCAGC+TGG + chr4_1:39364221-39364240 None:intergenic 45.0%
! GGTGAACTAGTTTCAATGGG+TGG - chr4_1:39364230-39364249 Msa0546880:intron 45.0%
!!! TTTTTATTGTATAAATATAA+TGG - chr4_1:39363360-39363379 Msa0546880:intron 5.0%
!!! TTTTTATTGTATAAATATAA+TGG - chr4_1:39363360-39363379 Msa0546880:intron 5.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 39362779 39364394 39362779 ID=Msa0546880;Name=Msa0546880
chr4_1 mRNA 39362779 39364394 39362779 ID=Msa0546880-mRNA-1;Parent=Msa0546880;Name=Msa0546880-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|1|0|1|1|1|2|0|114
chr4_1 exon 39364284 39364394 39364284 ID=Msa0546880-mRNA-1:exon:10011;Parent=Msa0546880-mRNA-1
chr4_1 exon 39362779 39363012 39362779 ID=Msa0546880-mRNA-1:exon:10010;Parent=Msa0546880-mRNA-1
chr4_1 CDS 39364284 39364394 39364284 ID=Msa0546880-mRNA-1:cds;Parent=Msa0546880-mRNA-1
chr4_1 CDS 39362779 39363012 39362779 ID=Msa0546880-mRNA-1:cds;Parent=Msa0546880-mRNA-1
Gene Sequence

>Msa0546880

ATGCCTTGCCTTTACATCTCTACCAACGTTAATTTAGATGGAAAAAACATCGATCCTATCTTTGATCAAGCTAGAACTGCTGTTTCCACAATCATAGGAAAACCCGACAAGTTTGTGATGGTTCTATTGAAGGGGTCAGTTCCAATATCGTTTGAAAATAACAAAGAACCAGCTGCATATGGTGAACTAGTTTCAATGGGTGGCATTAATTCAGAAGTGAAGAAGAATCTAATCAGTACCATTGGCACAATATTGGAATCCAACCTCTCTATACCAAGAACAAGATTTTTTCTCAAAGTCTTTGACATCATGGTTCTCAAAATTGAAAATAGCTCCAAACTGTAG

Protein sequence

>Msa0546880

MPCLYISTNVNLDGKNIDPIFDQARTAVSTIIGKPDKFVMVLLKGSVPISFENNKEPAAYGELVSMGGINSEVKKNLISTIGTILESNLSIPRTRFFLKVFDIMVLKIENSSKL*