AlfalfaGEDB | Msa0225310

M. sativa cultivar zhongmu-4 / Msa0225310

KEGG: K10418
GO: GO:0000003 GO:0000775 GO:0000776 GO:0001505 GO:0002791 GO:0002793 GO:0003006 GO:0003674 GO:0004857 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005868 GO:0005875 GO:0006807 GO:0006809 GO:0006810 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0008092 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010646 GO:0010647 GO:0010817 GO:0010970 GO:0012505 GO:0015630 GO:0016043 GO:0017144 GO:0019899 GO:0019953 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023056 GO:0030030 GO:0030031 GO:0030141 GO:0030154 GO:0030234 GO:0030235 GO:0030286 GO:0030424 GO:0030705 GO:0031410 GO:0031982 GO:0032024 GO:0032501 GO:0032502 GO:0032504 GO:0032781 GO:0032838 GO:0032879 GO:0032880 GO:0032991 GO:0033267 GO:0034641 GO:0035774 GO:0042133 GO:0042136 GO:0042802 GO:0042803 GO:0042995 GO:0043005 GO:0043085 GO:0043086 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043462 GO:0044085 GO:0044092 GO:0044093 GO:0044237 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044703 GO:0044782 GO:0045505 GO:0046209 GO:0046883 GO:0046887 GO:0046907 GO:0046982 GO:0046983 GO:0048232 GO:0048468 GO:0048515 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048609 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050796 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051704 GO:0051959 GO:0060271 GO:0060341 GO:0061178 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070925 GO:0071840 GO:0072593 GO:0072686 GO:0090087 GO:0090276 GO:0090277 GO:0097110 GO:0097458 GO:0097708 GO:0098687 GO:0098772 GO:0099111 GO:0099503 GO:0099568 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:0120111 GO:1902494 GO:1903409 GO:1903530 GO:1903532 GO:1904115 GO:1904951 GO:2000574 GO:2000576 GO:2000580 GO:2000582 GO:2001057
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225310	XP_003597748.1	100.000	91	0	0	1	91	1	91	6.60e-61	190

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225310	sp\|Q94748\|DYL2_SCHMA	46.667	90	46	1	1	90	1	88	7.02e-22	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225310	G7II18	100.000	91	0	0	1	91	1	91	3.15e-61	190

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0225310	0.814211	1.729155e-51	-8.615850e-47
Msa0110970	Msa0225310	0.804782	1.831539e-49	-8.615850e-47
Msa0147560	Msa0225310	0.818672	1.734662e-52	-8.615850e-47
Msa0157290	Msa0225310	0.814441	1.537878e-51	-8.615850e-47
Msa0193120	Msa0225310	0.806983	6.310578e-50	-8.615850e-47
Msa0225310	Msa0320100	0.805974	1.030214e-49	-8.615850e-47
Msa0225310	Msa0395960	0.812775	3.577044e-51	-8.615850e-47
Msa0225310	Msa0443500	0.819196	1.318554e-52	-8.615850e-47
Msa0225310	Msa0607200	0.822911	1.838633e-53	-8.615850e-47
Msa0225310	Msa0689010	0.800307	1.532801e-48	-8.615850e-47
Msa0225310	Msa0734660	0.822085	2.860866e-53	-8.615850e-47
Msa0225310	Msa0743720	0.802420	5.657907e-49	-8.615850e-47
Msa0225310	Msa0776220	0.828271	9.864463e-55	-8.615850e-47
Msa0225310	Msa0813670	0.834548	2.811811e-56	-8.615850e-47
Msa0225310	Msa0813720	0.836103	1.138677e-56	-8.615850e-47
Msa0225310	Msa0813740	0.824681	7.074400e-54	-8.615850e-47
Msa0225310	Msa0880140	0.816186	6.296161e-52	-8.615850e-47
Msa0225310	Msa1038050	0.809626	1.724262e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225310	MtrunA17_Chr2g0331651	100.000	91	0	0	1	91	1	91	6.07e-65	190
Msa0225310	MtrunA17_Chr6g0485891	75.556	90	22	0	1	90	35	124	6.64e-50	153
Msa0225310	MtrunA17_Chr8g0360861	54.444	90	41	0	1	90	1	90	5.40e-29	100
Msa0225310	MtrunA17_Chr4g0063491	49.367	79	40	0	11	89	159	237	3.99e-22	86.3
Msa0225310	MtrunA17_Chr4g0059791	46.067	89	46	2	2	89	213	300	9.46e-21	83.6
Msa0225310	MtrunA17_Chr8g0391451	41.667	84	47	1	8	89	56	139	2.40e-18	74.3
Msa0225310	MtrunA17_Chr4g0067531	40.698	86	49	1	5	90	46	129	2.60e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225310	AT1G52245.1	84.444	90	14	0	1	90	1	90	2.08e-55	166
Msa0225310	AT3G16120.2	78.889	90	19	0	1	90	1	90	4.93e-50	153
Msa0225310	AT3G16120.1	78.889	90	19	0	1	90	1	90	4.93e-50	153
Msa0225310	AT4G27360.1	54.444	90	41	0	1	90	1	90	1.41e-30	104
Msa0225310	AT5G20110.1	50.000	78	39	0	12	89	123	200	4.43e-20	80.5
Msa0225310	AT4G15930.1	42.353	85	47	1	5	89	39	121	1.08e-18	74.7
Msa0225310	AT1G23220.1	38.636	88	53	1	3	89	33	120	3.52e-18	73.6
Msa0225310	AT4G15930.2	44.262	61	32	1	5	65	39	97	6.83e-11	54.3

Find 34 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGATCTCCTTTCAGTTCT+TGG	0.156677	2_1:-75429230	Msa0225310:five_prime_UTR
CTCAAACTCCTTTGAAAATA+AGG	0.252205	2_1:+75428745	None:intergenic
ACTGATACTATATGCCTATA+TGG	0.309346	2_1:+75429398	None:intergenic
TGTGTGGTAGGTTCAAGTTT+TGG	0.339581	2_1:-75428828	Msa0225310:CDS
GGTTGCATTCCTTATGTTAA+GGG	0.349013	2_1:+75429341	None:intergenic
TGGTTGCATTCCTTATGTTA+AGG	0.354908	2_1:+75429340	None:intergenic
AATATAATCTCCATCTGAAA+AGG	0.387557	2_1:+75428615	None:intergenic
AATAGTAAAATATGCCATAT+AGG	0.414358	2_1:-75429412	Msa0225310:five_prime_UTR
TGAGTCATTCCCTTAACATA+AGG	0.417873	2_1:-75429350	Msa0225310:five_prime_UTR
GTATCAGTGCTAGCATGATA+TGG	0.430673	2_1:-75429383	Msa0225310:five_prime_UTR
GCTTGAATCTGCATCTTAGT+TGG	0.432610	2_1:+75429142	None:intergenic
TCCTCATGTCATATCATTCA+AGG	0.462204	2_1:-75428689	Msa0225310:three_prime_UTR
AACAGTAACTTCAAAGATGT+TGG	0.466683	2_1:-75429197	Msa0225310:exon
ATATAATCTCCATCTGAAAA+GGG	0.485076	2_1:+75428616	None:intergenic
TAGGAGTTTGATGCAAGATA+TGG	0.489901	2_1:-75428864	Msa0225310:CDS
GGTGGATGGCAATGTGTGGT+AGG	0.511909	2_1:-75428840	Msa0225310:CDS
TTCAAAACATTACAAAGGTA+AGG	0.524592	2_1:+75428554	None:intergenic
CTCAAAATCTCTGTAACCAA+AGG	0.540762	2_1:-75429444	Msa0225310:five_prime_UTR
CTTCATCTCTTCCCTCCATG+AGG	0.552282	2_1:-75428713	Msa0225310:three_prime_UTR
TAAGATGCAGATTCAAGCCA+TGG	0.595332	2_1:-75429137	Msa0225310:CDS
ACTTATCTCCAAGAACTGAA+AGG	0.602504	2_1:+75429222	None:intergenic
GTAACTTCAAAGATGTTGGA+AGG	0.606545	2_1:-75429193	Msa0225310:exon
ATATGACATGAGGATCCTCA+TGG	0.612359	2_1:+75428698	None:intergenic
AGCTTCAGAAGCATAAGCCA+TGG	0.620640	2_1:+75429120	None:intergenic
GACATGAGGATCCTCATGGA+GGG	0.624104	2_1:+75428702	None:intergenic
GAGTTTGATGCAAGATATGG+TGG	0.629614	2_1:-75428861	Msa0225310:CDS
CCCTTGAATGATATGACATG+AGG	0.633466	2_1:+75428688	None:intergenic
CCTCATGTCATATCATTCAA+GGG	0.635281	2_1:-75428688	Msa0225310:three_prime_UTR
TTTGATGCAAGATATGGTGG+TGG	0.642657	2_1:-75428858	Msa0225310:CDS
CACCCTTCAAAACATTACAA+AGG	0.650503	2_1:+75428549	None:intergenic
TGGTGGTGGATGGCAATGTG+TGG	0.654380	2_1:-75428844	Msa0225310:CDS
ATGCAAGATATGGTGGTGGA+TGG	0.656816	2_1:-75428854	Msa0225310:CDS
TGACATGAGGATCCTCATGG+AGG	0.660538	2_1:+75428701	None:intergenic
AGATAGACTTTAAAATGACA+CGG	0.680770	2_1:+75429308	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TACTATTTTTATTTTTCCTT+TGG	+	chr2_1:75428498-75428517	None:intergenic	15.0%
!!!	TACTATTTTTATTTTTCCTT+TGG	+	chr2_1:75428498-75428517	None:intergenic	15.0%
!!!	TTTTGAGCATTTTCTATTAA+TGG	+	chr2_1:75428466-75428485	None:intergenic	20.0%
!!	AATAGTAAAATATGCCATAT+AGG	-	chr2_1:75428511-75428530	Msa0225310:three_prime_UTR	20.0%
!!!	AAATGTAACCTTTTTTATGT+GGG	+	chr2_1:75428869-75428888	None:intergenic	20.0%
!!!	AAAATGTAACCTTTTTTATG+TGG	+	chr2_1:75428870-75428889	None:intergenic	20.0%
!!!	TTTTGAGCATTTTCTATTAA+TGG	+	chr2_1:75428466-75428485	None:intergenic	20.0%
!!	AATAGTAAAATATGCCATAT+AGG	-	chr2_1:75428511-75428530	Msa0225310:three_prime_UTR	20.0%
!!!	AAATGTAACCTTTTTTATGT+GGG	+	chr2_1:75428869-75428888	None:intergenic	20.0%
!!!	AAAATGTAACCTTTTTTATG+TGG	+	chr2_1:75428870-75428889	None:intergenic	20.0%
!!!	AGATAGACTTTAAAATGACA+CGG	+	chr2_1:75428618-75428637	None:intergenic	25.0%
!!!	CTATTTTGAAGACTAAAGAA+AGG	+	chr2_1:75428646-75428665	None:intergenic	25.0%
!!	TTTTCTTTGAATGTCATGTT+AGG	-	chr2_1:75429040-75429059	Msa0225310:intron	25.0%
!!!	TGTTTTTTCACTCATTCTAA+GGG	-	chr2_1:75429119-75429138	Msa0225310:CDS	25.0%
!!!	CTCAATTTCCTTATTTTCAA+AGG	-	chr2_1:75429170-75429189	Msa0225310:CDS	25.0%
!	ATATAATCTCCATCTGAAAA+GGG	+	chr2_1:75429310-75429329	None:intergenic	25.0%
!	AATATAATCTCCATCTGAAA+AGG	+	chr2_1:75429311-75429330	None:intergenic	25.0%
!	TTCAAAACATTACAAAGGTA+AGG	+	chr2_1:75429372-75429391	None:intergenic	25.0%
!!!	TTACCTTTGTAATGTTTTGA+AGG	-	chr2_1:75429371-75429390	Msa0225310:five_prime_UTR	25.0%
!!!	TACCTTTGTAATGTTTTGAA+GGG	-	chr2_1:75429372-75429391	Msa0225310:five_prime_UTR	25.0%
!!!	AGATAGACTTTAAAATGACA+CGG	+	chr2_1:75428618-75428637	None:intergenic	25.0%
!!!	CTATTTTGAAGACTAAAGAA+AGG	+	chr2_1:75428646-75428665	None:intergenic	25.0%
!!	TTTTCTTTGAATGTCATGTT+AGG	-	chr2_1:75429040-75429059	Msa0225310:intron	25.0%
!!!	TGTTTTTTCACTCATTCTAA+GGG	-	chr2_1:75429119-75429138	Msa0225310:CDS	25.0%
!!!	CTCAATTTCCTTATTTTCAA+AGG	-	chr2_1:75429170-75429189	Msa0225310:CDS	25.0%
!	ATATAATCTCCATCTGAAAA+GGG	+	chr2_1:75429310-75429329	None:intergenic	25.0%
!	AATATAATCTCCATCTGAAA+AGG	+	chr2_1:75429311-75429330	None:intergenic	25.0%
!	TTCAAAACATTACAAAGGTA+AGG	+	chr2_1:75429372-75429391	None:intergenic	25.0%
!!!	TTACCTTTGTAATGTTTTGA+AGG	-	chr2_1:75429371-75429390	Msa0225310:five_prime_UTR	25.0%
!!!	TACCTTTGTAATGTTTTGAA+GGG	-	chr2_1:75429372-75429391	Msa0225310:five_prime_UTR	25.0%
	ACTGATACTATATGCCTATA+TGG	+	chr2_1:75428528-75428547	None:intergenic	30.0%
	AAATGACACGGAAAAAAACA+TGG	+	chr2_1:75428606-75428625	None:intergenic	30.0%
	AACAGTAACTTCAAAGATGT+TGG	-	chr2_1:75428726-75428745	Msa0225310:three_prime_UTR	30.0%
!!!	GTGTTTTTTCACTCATTCTA+AGG	-	chr2_1:75429118-75429137	Msa0225310:CDS	30.0%
!	CTCAAACTCCTTTGAAAATA+AGG	+	chr2_1:75429181-75429200	None:intergenic	30.0%
	ACTGATACTATATGCCTATA+TGG	+	chr2_1:75428528-75428547	None:intergenic	30.0%
	AAATGACACGGAAAAAAACA+TGG	+	chr2_1:75428606-75428625	None:intergenic	30.0%
	AACAGTAACTTCAAAGATGT+TGG	-	chr2_1:75428726-75428745	Msa0225310:three_prime_UTR	30.0%
!!!	GTGTTTTTTCACTCATTCTA+AGG	-	chr2_1:75429118-75429137	Msa0225310:CDS	30.0%
!	CTCAAACTCCTTTGAAAATA+AGG	+	chr2_1:75429181-75429200	None:intergenic	30.0%
	CTCAAAATCTCTGTAACCAA+AGG	-	chr2_1:75428479-75428498	Msa0225310:three_prime_UTR	35.0%
	TGAGTCATTCCCTTAACATA+AGG	-	chr2_1:75428573-75428592	Msa0225310:three_prime_UTR	35.0%
	GGTTGCATTCCTTATGTTAA+GGG	+	chr2_1:75428585-75428604	None:intergenic	35.0%
	TGGTTGCATTCCTTATGTTA+AGG	+	chr2_1:75428586-75428605	None:intergenic	35.0%
	TTAGATCTCCTTTCAGTTCT+TGG	-	chr2_1:75428693-75428712	Msa0225310:three_prime_UTR	35.0%
	ACTTATCTCCAAGAACTGAA+AGG	+	chr2_1:75428704-75428723	None:intergenic	35.0%
	GTAACTTCAAAGATGTTGGA+AGG	-	chr2_1:75428730-75428749	Msa0225310:exon	35.0%
	TAGGAGTTTGATGCAAGATA+TGG	-	chr2_1:75429059-75429078	Msa0225310:intron	35.0%
	TCCTCATGTCATATCATTCA+AGG	-	chr2_1:75429234-75429253	Msa0225310:five_prime_UTR	35.0%
	CCTCATGTCATATCATTCAA+GGG	-	chr2_1:75429235-75429254	Msa0225310:five_prime_UTR	35.0%
	GGGCTAAGAAAAAAGGATAA+AGG	+	chr2_1:75429290-75429309	None:intergenic	35.0%
	CTGAAAAGGGCTAAGAAAAA+AGG	+	chr2_1:75429297-75429316	None:intergenic	35.0%
!	TTTTCTTAGCCCTTTTCAGA+TGG	-	chr2_1:75429298-75429317	Msa0225310:five_prime_UTR	35.0%
	CACCCTTCAAAACATTACAA+AGG	+	chr2_1:75429377-75429396	None:intergenic	35.0%
	CTCAAAATCTCTGTAACCAA+AGG	-	chr2_1:75428479-75428498	Msa0225310:three_prime_UTR	35.0%
	TGAGTCATTCCCTTAACATA+AGG	-	chr2_1:75428573-75428592	Msa0225310:three_prime_UTR	35.0%
	GGTTGCATTCCTTATGTTAA+GGG	+	chr2_1:75428585-75428604	None:intergenic	35.0%
	TGGTTGCATTCCTTATGTTA+AGG	+	chr2_1:75428586-75428605	None:intergenic	35.0%
	TTAGATCTCCTTTCAGTTCT+TGG	-	chr2_1:75428693-75428712	Msa0225310:three_prime_UTR	35.0%
	ACTTATCTCCAAGAACTGAA+AGG	+	chr2_1:75428704-75428723	None:intergenic	35.0%
	GTAACTTCAAAGATGTTGGA+AGG	-	chr2_1:75428730-75428749	Msa0225310:exon	35.0%
	TAGGAGTTTGATGCAAGATA+TGG	-	chr2_1:75429059-75429078	Msa0225310:intron	35.0%
	TCCTCATGTCATATCATTCA+AGG	-	chr2_1:75429234-75429253	Msa0225310:five_prime_UTR	35.0%
	CCTCATGTCATATCATTCAA+GGG	-	chr2_1:75429235-75429254	Msa0225310:five_prime_UTR	35.0%
	GGGCTAAGAAAAAAGGATAA+AGG	+	chr2_1:75429290-75429309	None:intergenic	35.0%
	CTGAAAAGGGCTAAGAAAAA+AGG	+	chr2_1:75429297-75429316	None:intergenic	35.0%
!	TTTTCTTAGCCCTTTTCAGA+TGG	-	chr2_1:75429298-75429317	Msa0225310:five_prime_UTR	35.0%
	CACCCTTCAAAACATTACAA+AGG	+	chr2_1:75429377-75429396	None:intergenic	35.0%
	GTATCAGTGCTAGCATGATA+TGG	-	chr2_1:75428540-75428559	Msa0225310:three_prime_UTR	40.0%
	GCTTGAATCTGCATCTTAGT+TGG	+	chr2_1:75428784-75428803	None:intergenic	40.0%
	TAAGATGCAGATTCAAGCCA+TGG	-	chr2_1:75428786-75428805	Msa0225310:CDS	40.0%
!!!	CTTTTTTATGTGGGCAGCAA+TGG	+	chr2_1:75428860-75428879	None:intergenic	40.0%
	CATTGCTGCCCACATAAAAA+AGG	-	chr2_1:75428858-75428877	Msa0225310:CDS	40.0%
	GAGTTTGATGCAAGATATGG+TGG	-	chr2_1:75429062-75429081	Msa0225310:intron	40.0%
	TTTGATGCAAGATATGGTGG+TGG	-	chr2_1:75429065-75429084	Msa0225310:intron	40.0%
!!	TGTGTGGTAGGTTCAAGTTT+TGG	-	chr2_1:75429095-75429114	Msa0225310:CDS	40.0%
!!	GTGTGGTAGGTTCAAGTTTT+GGG	-	chr2_1:75429096-75429115	Msa0225310:CDS	40.0%
	ATATGACATGAGGATCCTCA+TGG	+	chr2_1:75429228-75429247	None:intergenic	40.0%
	CCCTTGAATGATATGACATG+AGG	+	chr2_1:75429238-75429257	None:intergenic	40.0%
	GTATCAGTGCTAGCATGATA+TGG	-	chr2_1:75428540-75428559	Msa0225310:three_prime_UTR	40.0%
	GCTTGAATCTGCATCTTAGT+TGG	+	chr2_1:75428784-75428803	None:intergenic	40.0%
	TAAGATGCAGATTCAAGCCA+TGG	-	chr2_1:75428786-75428805	Msa0225310:CDS	40.0%
!!!	CTTTTTTATGTGGGCAGCAA+TGG	+	chr2_1:75428860-75428879	None:intergenic	40.0%
	CATTGCTGCCCACATAAAAA+AGG	-	chr2_1:75428858-75428877	Msa0225310:CDS	40.0%
	GAGTTTGATGCAAGATATGG+TGG	-	chr2_1:75429062-75429081	Msa0225310:intron	40.0%
	TTTGATGCAAGATATGGTGG+TGG	-	chr2_1:75429065-75429084	Msa0225310:intron	40.0%
!!	TGTGTGGTAGGTTCAAGTTT+TGG	-	chr2_1:75429095-75429114	Msa0225310:CDS	40.0%
!!	GTGTGGTAGGTTCAAGTTTT+GGG	-	chr2_1:75429096-75429115	Msa0225310:CDS	40.0%
	ATATGACATGAGGATCCTCA+TGG	+	chr2_1:75429228-75429247	None:intergenic	40.0%
	CCCTTGAATGATATGACATG+AGG	+	chr2_1:75429238-75429257	None:intergenic	40.0%
!	AGCTTCAGAAGCATAAGCCA+TGG	+	chr2_1:75428806-75428825	None:intergenic	45.0%
!!	ATGCAAGATATGGTGGTGGA+TGG	-	chr2_1:75429069-75429088	Msa0225310:CDS	45.0%
!	AGCTTCAGAAGCATAAGCCA+TGG	+	chr2_1:75428806-75428825	None:intergenic	45.0%
!!	ATGCAAGATATGGTGGTGGA+TGG	-	chr2_1:75429069-75429088	Msa0225310:CDS	45.0%
	CTTCATCTCTTCCCTCCATG+AGG	-	chr2_1:75429210-75429229	Msa0225310:five_prime_UTR	50.0%
	GACATGAGGATCCTCATGGA+GGG	+	chr2_1:75429224-75429243	None:intergenic	50.0%
	TGACATGAGGATCCTCATGG+AGG	+	chr2_1:75429225-75429244	None:intergenic	50.0%
	CTTCATCTCTTCCCTCCATG+AGG	-	chr2_1:75429210-75429229	Msa0225310:five_prime_UTR	50.0%
	GACATGAGGATCCTCATGGA+GGG	+	chr2_1:75429224-75429243	None:intergenic	50.0%
	TGACATGAGGATCCTCATGG+AGG	+	chr2_1:75429225-75429244	None:intergenic	50.0%
!!	TGGTGGTGGATGGCAATGTG+TGG	-	chr2_1:75429079-75429098	Msa0225310:CDS	55.0%
	GGTGGATGGCAATGTGTGGT+AGG	-	chr2_1:75429083-75429102	Msa0225310:CDS	55.0%
!!	TGGTGGTGGATGGCAATGTG+TGG	-	chr2_1:75429079-75429098	Msa0225310:CDS	55.0%
	GGTGGATGGCAATGTGTGGT+AGG	-	chr2_1:75429083-75429102	Msa0225310:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	75428445	75429500	75428445	ID=Msa0225310;Name=Msa0225310
chr2_1	mRNA	75428445	75429500	75428445	ID=Msa0225310-mRNA-1;Parent=Msa0225310;Name=Msa0225310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=297\|1\|1\|1\|1\|1\|2\|301\|91
chr2_1	exon	75428445	75428883	75428445	ID=Msa0225310-mRNA-1:exon:22562;Parent=Msa0225310-mRNA-1
chr2_1	exon	75429066	75429500	75429066	ID=Msa0225310-mRNA-1:exon:22561;Parent=Msa0225310-mRNA-1
chr2_1	five_prime_UTR	75429204	75429500	75429204	ID=Msa0225310-mRNA-1:five_prime_utr;Parent=Msa0225310-mRNA-1
chr2_1	CDS	75429066	75429203	75429066	ID=Msa0225310-mRNA-1:cds;Parent=Msa0225310-mRNA-1
chr2_1	CDS	75428746	75428883	75428746	ID=Msa0225310-mRNA-1:cds;Parent=Msa0225310-mRNA-1
chr2_1	three_prime_UTR	75428445	75428745	75428445	ID=Msa0225310-mRNA-1:three_prime_utr;Parent=Msa0225310-mRNA-1

Gene Sequence	>Msa0225310 ATGTTGGAAGGTAAAGCTATGATAGAAGATACTGACATGCCAACTAAGATGCAGATTCAAGCCATGGCTTATGCTTCTGAAGCTCTTGATCTATATGATGTTATTGATTGCAAATCCATTGCTGCCCACATAAAAAAGGAGTTTGATGCAAGATATGGTGGTGGATGGCAATGTGTGGTAGGTTCAAGTTTTGGGTGTTTTTTCACTCATTCTAAGGGAACTTTCATATACTTCACTCTTGAGACTCTCAATTTCCTTATTTTCAAAGGAGTTTGA
Protein sequence	>Msa0225310 MLEGKAMIEDTDMPTKMQIQAMAYASEALDLYDVIDCKSIAAHIKKEFDARYGGGWQCVVGSSFGCFFTHSKGTFIYFTLETLNFLIFKGV*