AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0243140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0243140 MtrunA17_Chr2g0321121 96.460 113 4 0 1 113 1 113 3.55e-70 205
Msa0243140 MtrunA17_Chr5g0421491 87.611 113 14 0 1 113 1 113 5.16e-38 124
Msa0243140 MtrunA17_Chr1g0179001 73.451 113 29 1 1 113 1 112 8.39e-30 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0243140 AT2G27710.4 77.143 70 16 0 1 70 1 70 9.36e-32 108
Msa0243140 AT3G44590.1 67.257 113 35 2 1 113 1 111 8.38e-30 103
Msa0243140 AT3G44590.2 67.257 113 35 2 1 113 1 111 8.38e-30 103
Msa0243140 AT2G27720.1 66.087 115 37 1 1 113 1 115 1.93e-27 97.8
Msa0243140 AT2G27710.1 75.385 65 16 0 1 65 1 65 2.45e-25 92.4
Msa0243140 AT2G27710.2 75.385 65 16 0 1 65 1 65 2.45e-25 92.4
Msa0243140 AT2G27710.3 75.385 65 16 0 1 65 1 65 2.45e-25 92.4
Msa0243140 AT3G28500.1 55.652 115 49 2 1 113 1 115 3.14e-25 92.0
Msa0243140 AT2G27720.3 59.843 127 37 2 1 113 1 127 6.69e-24 89.0
Msa0243140 AT2G27720.2 57.692 130 38 2 1 113 1 130 1.44e-22 85.9
Msa0243140 AT5G40040.1 57.895 114 47 1 1 113 1 114 2.30e-16 69.3
Msa0243140 AT2G27720.4 66.667 87 27 1 29 113 5 91 5.67e-13 60.1

Find 51 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTACTTGTTGGCCGTTT+TGG 0.128749 2_2:-19993276 Msa0243140:CDS
ATTCAGCTTATTTGATTAAA+AGG 0.186887 2_2:-19991820 Msa0243140:exon
GCTGATCTTAAACAAATTCT+TGG 0.264686 2_2:-19993230 Msa0243140:CDS
GTTGCTGTGTCTGCTGCAAC+TGG 0.362343 2_2:-19992017 Msa0243140:CDS
TCTTCCTTTGCTGCTTCAGC+AGG 0.362525 2_2:+19991972 None:intergenic
CTTAAACAAATTCTTGGATC+AGG 0.372982 2_2:-19993224 Msa0243140:intron
TTTCAATTTAATCTGCGTTA+TGG 0.375448 2_2:-19991682 Msa0243140:three_prime_UTR
GTTATCTTTGTGCAGGATAT+GGG 0.399106 2_2:-19991843 Msa0243140:intron
AGAGAAGAAGGTGGAAGAGA+AGG 0.434796 2_2:-19991952 Msa0243140:CDS
TGCGGCTTGCAGCATCCCTC+TGG 0.441220 2_2:-19993458 Msa0243140:five_prime_UTR
GATTATCGCAGCTTACTTGT+TGG 0.445399 2_2:-19993285 Msa0243140:CDS
GTGTCTGCTGCAACTGGAGG+AGG 0.445905 2_2:-19992011 Msa0243140:CDS
CCAAGTTGATATTTATTGAC+TGG 0.461135 2_2:-19991491 Msa0243140:three_prime_UTR
GATCAGCAGCAGAAGGGGAT+TGG 0.472152 2_2:+19993246 None:intergenic
GATATCTTACTTGATGGAAA+TGG 0.483487 2_2:+19993402 None:intergenic
CCTTGCCCTTGACTTCACTT+AGG 0.488662 2_2:+19992105 None:intergenic
TGCCAGTGGAAGAGAAAAGT+TGG 0.491656 2_2:-19992066 Msa0243140:CDS
TTGACTGGTACACCAGATGT+AGG 0.496222 2_2:-19991476 Msa0243140:three_prime_UTR
CACAGCAACAGCACCACCAC+CGG 0.500128 2_2:+19992031 None:intergenic
AGCAGCAAAGGAAGAGAAGA+AGG 0.518126 2_2:-19991964 Msa0243140:CDS
GCTTCAGTTCCATCCGGTGG+TGG 0.522209 2_2:-19992044 Msa0243140:CDS
CCAGTCAATAAATATCAACT+TGG 0.526486 2_2:+19991491 None:intergenic
TGCACCTGCTGAAGCAGCAA+AGG 0.528481 2_2:-19991976 Msa0243140:CDS
GGATTGGTTGCCTCCCAAAA+CGG 0.534645 2_2:+19993262 None:intergenic
CGGATGAGATCTCGACTTGA+CGG 0.535390 2_2:+19993425 None:intergenic
CAACTCAATGTTGCCATCCT+CGG 0.536919 2_2:+19992130 None:intergenic
GCAACAGCACCACCACCGGA+TGG 0.539099 2_2:+19992035 None:intergenic
TGTTTAAGATCAGCAGCAGA+AGG 0.545696 2_2:+19993239 None:intergenic
TCTTCCACTGGCAATAAGCT+CGG 0.547245 2_2:+19992076 None:intergenic
TGAGATCTCGACTTGACGGT+AGG 0.564173 2_2:+19993429 None:intergenic
AAGTTGGCTTCAGTTCCATC+CGG 0.567453 2_2:-19992050 Msa0243140:CDS
ATCTTACTTGATGGAAATGG+CGG 0.571234 2_2:+19993405 None:intergenic
GACGGTAGGGTTTCACCAGA+GGG 0.572119 2_2:+19993443 None:intergenic
GTTGGAGTTGAGGCCGAGGA+TGG 0.573673 2_2:-19992143 Msa0243140:CDS
TTTAAGATCAGCAGCAGAAG+GGG 0.576420 2_2:+19993241 None:intergenic
GAGATCTCGACTTGACGGTA+GGG 0.582460 2_2:+19993430 None:intergenic
TGACTGGTACACCAGATGTA+GGG 0.583188 2_2:-19991475 None:intergenic
GTTTAAGATCAGCAGCAGAA+GGG 0.590692 2_2:+19993240 None:intergenic
GTTGCTCCTAAGTGAAGTCA+AGG 0.590863 2_2:-19992111 Msa0243140:CDS
GCTGTGTCTGCTGCAACTGG+AGG 0.592795 2_2:-19992014 Msa0243140:CDS
ATTGTCTTAATATTATTGCA+TGG 0.594069 2_2:-19991517 Msa0243140:three_prime_UTR
TATGATTGTAGTTGGAGTTG+AGG 0.599920 2_2:-19992153 Msa0243140:intron
CATGAATCAACCACTAGAGG+AGG 0.604458 2_2:+19991601 None:intergenic
TTTGCCGAGCTTATTGCCAG+TGG 0.606365 2_2:-19992080 Msa0243140:CDS
TTGGCTTCAGTTCCATCCGG+TGG 0.607108 2_2:-19992047 Msa0243140:CDS
TGACGGTAGGGTTTCACCAG+AGG 0.609194 2_2:+19993442 None:intergenic
ATTCATGAATCAACCACTAG+AGG 0.615895 2_2:+19991598 None:intergenic
CCTAAGTGAAGTCAAGGGCA+AGG 0.616488 2_2:-19992105 Msa0243140:CDS
TGTAGTTGGAGTTGAGGCCG+AGG 0.617745 2_2:-19992147 Msa0243140:intron
TTGCTCCTAAGTGAAGTCAA+GGG 0.680870 2_2:-19992110 Msa0243140:CDS
AGCAAAGGAAGAGAAGAAGG+TGG 0.683753 2_2:-19991961 Msa0243140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGATTAAAGATTAAATT+GGG + chr2_2:19991746-19991765 None:intergenic 10.0%
!! AATAAGATTAAAGATTAAAT+TGG + chr2_2:19991747-19991766 None:intergenic 10.0%
!!! AAATAATTTTAATTCAAGTT+TGG - chr2_2:19992150-19992169 Msa0243140:intron 10.0%
!!! AATAATTTTAATTCAAGTTT+GGG - chr2_2:19992151-19992170 Msa0243140:intron 10.0%
!! ATAAGATTAAAGATTAAATT+GGG + chr2_2:19991746-19991765 None:intergenic 10.0%
!! AATAAGATTAAAGATTAAAT+TGG + chr2_2:19991747-19991766 None:intergenic 10.0%
!!! AAATAATTTTAATTCAAGTT+TGG - chr2_2:19992150-19992169 Msa0243140:intron 10.0%
!!! AATAATTTTAATTCAAGTTT+GGG - chr2_2:19992151-19992170 Msa0243140:intron 10.0%
!!! TTTAACTTTAATTCAAGTTT+GGG - chr2_2:19992184-19992203 Msa0243140:intron 15.0%
!! ATGAAAATTGATCAAAATAT+TGG - chr2_2:19992232-19992251 Msa0243140:intron 15.0%
!! TTAAAATCAAACCAATATAT+GGG + chr2_2:19992506-19992525 None:intergenic 15.0%
!! ATTAAAATCAAACCAATATA+TGG + chr2_2:19992507-19992526 None:intergenic 15.0%
!!! AAAAAAAGTGACTTTATTTT+TGG - chr2_2:19992754-19992773 Msa0243140:intron 15.0%
!!! AAAAAAGTGACTTTATTTTT+GGG - chr2_2:19992755-19992774 Msa0243140:intron 15.0%
!!! TTTAACTTTAATTCAAGTTT+GGG - chr2_2:19992184-19992203 Msa0243140:intron 15.0%
!! ATGAAAATTGATCAAAATAT+TGG - chr2_2:19992232-19992251 Msa0243140:intron 15.0%
!! TTAAAATCAAACCAATATAT+GGG + chr2_2:19992506-19992525 None:intergenic 15.0%
!! ATTAAAATCAAACCAATATA+TGG + chr2_2:19992507-19992526 None:intergenic 15.0%
!!! AAAAAAAGTGACTTTATTTT+TGG - chr2_2:19992754-19992773 Msa0243140:intron 15.0%
!!! AAAAAAGTGACTTTATTTTT+GGG - chr2_2:19992755-19992774 Msa0243140:intron 15.0%
!! ATGGATCTACTAAAATAATT+CGG + chr2_2:19991842-19991861 None:intergenic 20.0%
!!! ATATTCTACACTTTTACTTA+TGG + chr2_2:19991861-19991880 None:intergenic 20.0%
!!! TGCTAATTTAATTCAAGTTT+AGG - chr2_2:19991987-19992006 Msa0243140:CDS 20.0%
!!! GGATTTGTTTAATTGATAAT+CGG - chr2_2:19992008-19992027 Msa0243140:CDS 20.0%
!!! GTTTAACTTTAATTCAAGTT+TGG - chr2_2:19992183-19992202 Msa0243140:intron 20.0%
!!! GATCAAAATATTGGTTTATT+TGG - chr2_2:19992241-19992260 Msa0243140:intron 20.0%
!!! AAATATTGGTTTATTTGGAT+TGG - chr2_2:19992246-19992265 Msa0243140:intron 20.0%
!! AATAGTTTATGCAAATTCAT+AGG - chr2_2:19992420-19992439 Msa0243140:intron 20.0%
!! TAAAATCAAACCAATATATG+GGG + chr2_2:19992505-19992524 None:intergenic 20.0%
!!! GTATATTTTTTTACTGTTGA+AGG - chr2_2:19992642-19992661 Msa0243140:intron 20.0%
!!! AATTTTGAAACTTACATCAT+CGG + chr2_2:19993013-19993032 None:intergenic 20.0%
!! ATTCAGCTTATTTGATTAAA+AGG - chr2_2:19993113-19993132 Msa0243140:intron 20.0%
!! ATTGTCTTAATATTATTGCA+TGG - chr2_2:19993416-19993435 Msa0243140:five_prime_UTR 20.0%
!! ATGGATCTACTAAAATAATT+CGG + chr2_2:19991842-19991861 None:intergenic 20.0%
!!! ATATTCTACACTTTTACTTA+TGG + chr2_2:19991861-19991880 None:intergenic 20.0%
!!! TGCTAATTTAATTCAAGTTT+AGG - chr2_2:19991987-19992006 Msa0243140:CDS 20.0%
!!! GGATTTGTTTAATTGATAAT+CGG - chr2_2:19992008-19992027 Msa0243140:CDS 20.0%
!!! GTTTAACTTTAATTCAAGTT+TGG - chr2_2:19992183-19992202 Msa0243140:intron 20.0%
!!! GATCAAAATATTGGTTTATT+TGG - chr2_2:19992241-19992260 Msa0243140:intron 20.0%
!!! AAATATTGGTTTATTTGGAT+TGG - chr2_2:19992246-19992265 Msa0243140:intron 20.0%
!! AATAGTTTATGCAAATTCAT+AGG - chr2_2:19992420-19992439 Msa0243140:intron 20.0%
!! TAAAATCAAACCAATATATG+GGG + chr2_2:19992505-19992524 None:intergenic 20.0%
!!! GTATATTTTTTTACTGTTGA+AGG - chr2_2:19992642-19992661 Msa0243140:intron 20.0%
!!! AATTTTGAAACTTACATCAT+CGG + chr2_2:19993013-19993032 None:intergenic 20.0%
!! ATTCAGCTTATTTGATTAAA+AGG - chr2_2:19993113-19993132 Msa0243140:intron 20.0%
!! ATTGTCTTAATATTATTGCA+TGG - chr2_2:19993416-19993435 Msa0243140:five_prime_UTR 20.0%
! CAAACATTAAACAAAAACGA+TGG + chr2_2:19991615-19991634 None:intergenic 25.0%
! TTAAAATCGATGAATGAACA+TGG - chr2_2:19992036-19992055 Msa0243140:CDS 25.0%
! TAAAATCGATGAATGAACAT+GGG - chr2_2:19992037-19992056 Msa0243140:CDS 25.0%
!!! ATTTTAATTCAAGTTTGGGT+TGG - chr2_2:19992155-19992174 Msa0243140:intron 25.0%
!!! TTTTAATTCAAGTTTGGGTT+GGG - chr2_2:19992156-19992175 Msa0243140:intron 25.0%
!!! GCTTAGTTTTATACATTGTT+TGG - chr2_2:19992527-19992546 Msa0243140:intron 25.0%
!! TTTTACTGTTGAAGGAAAAA+TGG - chr2_2:19992650-19992669 Msa0243140:intron 25.0%
!! TAGTAGTATTGTTTGTTACT+TGG - chr2_2:19993035-19993054 Msa0243140:intron 25.0%
! TTTCAATTTAATCTGCGTTA+TGG - chr2_2:19993251-19993270 Msa0243140:CDS 25.0%
! CAAACATTAAACAAAAACGA+TGG + chr2_2:19991615-19991634 None:intergenic 25.0%
! TTAAAATCGATGAATGAACA+TGG - chr2_2:19992036-19992055 Msa0243140:CDS 25.0%
! TAAAATCGATGAATGAACAT+GGG - chr2_2:19992037-19992056 Msa0243140:CDS 25.0%
!!! ATTTTAATTCAAGTTTGGGT+TGG - chr2_2:19992155-19992174 Msa0243140:intron 25.0%
!!! TTTTAATTCAAGTTTGGGTT+GGG - chr2_2:19992156-19992175 Msa0243140:intron 25.0%
!!! GCTTAGTTTTATACATTGTT+TGG - chr2_2:19992527-19992546 Msa0243140:intron 25.0%
!! TTTTACTGTTGAAGGAAAAA+TGG - chr2_2:19992650-19992669 Msa0243140:intron 25.0%
!! TAGTAGTATTGTTTGTTACT+TGG - chr2_2:19993035-19993054 Msa0243140:intron 25.0%
! TTTCAATTTAATCTGCGTTA+TGG - chr2_2:19993251-19993270 Msa0243140:CDS 25.0%
GATATCTTACTTGATGGAAA+TGG + chr2_2:19991534-19991553 None:intergenic 30.0%
GAAATCGATATCTTACTTGA+TGG + chr2_2:19991540-19991559 None:intergenic 30.0%
! AAACTTCACTCTTTTACGTT+TGG - chr2_2:19991568-19991587 Msa0243140:three_prime_UTR 30.0%
! AACTTCACTCTTTTACGTTT+GGG - chr2_2:19991569-19991588 Msa0243140:three_prime_UTR 30.0%
GCTGATCTTAAACAAATTCT+TGG - chr2_2:19991703-19991722 Msa0243140:three_prime_UTR 30.0%
CTTAAACAAATTCTTGGATC+AGG - chr2_2:19991709-19991728 Msa0243140:three_prime_UTR 30.0%
!!! GCGTTTTTTGTTTGTTTTTC+CGG - chr2_2:19991800-19991819 Msa0243140:three_prime_UTR 30.0%
AAATAATCAATCCTAGACTG+TGG - chr2_2:19991943-19991962 Msa0243140:CDS 30.0%
AATAATCAATCCTAGACTGT+GGG - chr2_2:19991944-19991963 Msa0243140:CDS 30.0%
TATCTACTGTTAGACTGTTA+AGG - chr2_2:19992282-19992301 Msa0243140:intron 30.0%
CCAGTCAATAAATATCAACT+TGG + chr2_2:19993445-19993464 None:intergenic 30.0%
!! CCAAGTTGATATTTATTGAC+TGG - chr2_2:19993442-19993461 Msa0243140:five_prime_UTR 30.0%
GATATCTTACTTGATGGAAA+TGG + chr2_2:19991534-19991553 None:intergenic 30.0%
GAAATCGATATCTTACTTGA+TGG + chr2_2:19991540-19991559 None:intergenic 30.0%
! AAACTTCACTCTTTTACGTT+TGG - chr2_2:19991568-19991587 Msa0243140:three_prime_UTR 30.0%
! AACTTCACTCTTTTACGTTT+GGG - chr2_2:19991569-19991588 Msa0243140:three_prime_UTR 30.0%
GCTGATCTTAAACAAATTCT+TGG - chr2_2:19991703-19991722 Msa0243140:three_prime_UTR 30.0%
CTTAAACAAATTCTTGGATC+AGG - chr2_2:19991709-19991728 Msa0243140:three_prime_UTR 30.0%
!!! GCGTTTTTTGTTTGTTTTTC+CGG - chr2_2:19991800-19991819 Msa0243140:three_prime_UTR 30.0%
AAATAATCAATCCTAGACTG+TGG - chr2_2:19991943-19991962 Msa0243140:CDS 30.0%
AATAATCAATCCTAGACTGT+GGG - chr2_2:19991944-19991963 Msa0243140:CDS 30.0%
TATCTACTGTTAGACTGTTA+AGG - chr2_2:19992282-19992301 Msa0243140:intron 30.0%
CCAGTCAATAAATATCAACT+TGG + chr2_2:19993445-19993464 None:intergenic 30.0%
!! CCAAGTTGATATTTATTGAC+TGG - chr2_2:19993442-19993461 Msa0243140:five_prime_UTR 30.0%
ATCTTACTTGATGGAAATGG+CGG + chr2_2:19991531-19991550 None:intergenic 35.0%
TTATCTACTGTTGGACAGTT+TGG - chr2_2:19992086-19992105 Msa0243140:CDS 35.0%
TATCTACTGTTGGACAGTTT+GGG - chr2_2:19992087-19992106 Msa0243140:CDS 35.0%
AATCAAACAGCCCCATATAT+TGG - chr2_2:19992492-19992511 Msa0243140:intron 35.0%
TTTGGGTCTATGATTGTAGT+TGG - chr2_2:19992772-19992791 Msa0243140:intron 35.0%
TATGATTGTAGTTGGAGTTG+AGG - chr2_2:19992780-19992799 Msa0243140:intron 35.0%
TGTTATCTTTGTGCAGGATA+TGG - chr2_2:19993089-19993108 Msa0243140:intron 35.0%
GTTATCTTTGTGCAGGATAT+GGG - chr2_2:19993090-19993109 Msa0243140:intron 35.0%
ATTCATGAATCAACCACTAG+AGG + chr2_2:19993338-19993357 None:intergenic 35.0%
ATCTTACTTGATGGAAATGG+CGG + chr2_2:19991531-19991550 None:intergenic 35.0%
TTATCTACTGTTGGACAGTT+TGG - chr2_2:19992086-19992105 Msa0243140:CDS 35.0%
TATCTACTGTTGGACAGTTT+GGG - chr2_2:19992087-19992106 Msa0243140:CDS 35.0%
AATCAAACAGCCCCATATAT+TGG - chr2_2:19992492-19992511 Msa0243140:intron 35.0%
TTTGGGTCTATGATTGTAGT+TGG - chr2_2:19992772-19992791 Msa0243140:intron 35.0%
TATGATTGTAGTTGGAGTTG+AGG - chr2_2:19992780-19992799 Msa0243140:intron 35.0%
TGTTATCTTTGTGCAGGATA+TGG - chr2_2:19993089-19993108 Msa0243140:intron 35.0%
GTTATCTTTGTGCAGGATAT+GGG - chr2_2:19993090-19993109 Msa0243140:intron 35.0%
ATTCATGAATCAACCACTAG+AGG + chr2_2:19993338-19993357 None:intergenic 35.0%
GATTATCGCAGCTTACTTGT+TGG - chr2_2:19991648-19991667 Msa0243140:three_prime_UTR 40.0%
! TTTAAGATCAGCAGCAGAAG+GGG + chr2_2:19991695-19991714 None:intergenic 40.0%
! GTTTAAGATCAGCAGCAGAA+GGG + chr2_2:19991696-19991715 None:intergenic 40.0%
! TGTTTAAGATCAGCAGCAGA+AGG + chr2_2:19991697-19991716 None:intergenic 40.0%
CGGATACTAATCATCGAAAC+CGG + chr2_2:19991822-19991841 None:intergenic 40.0%
CTTCATTATACCCACAGTCT+AGG + chr2_2:19991957-19991976 None:intergenic 40.0%
TGAATGAACATGGGTCTGTT+TGG - chr2_2:19992046-19992065 Msa0243140:CDS 40.0%
CGTTTGAGCTTATCTACTGT+TGG - chr2_2:19992077-19992096 Msa0243140:CDS 40.0%
TTGCTCCTAAGTGAAGTCAA+GGG - chr2_2:19992823-19992842 Msa0243140:intron 40.0%
GCGTAATGTTATCTTTGTGC+AGG - chr2_2:19993083-19993102 Msa0243140:intron 40.0%
GATTATCGCAGCTTACTTGT+TGG - chr2_2:19991648-19991667 Msa0243140:three_prime_UTR 40.0%
! TTTAAGATCAGCAGCAGAAG+GGG + chr2_2:19991695-19991714 None:intergenic 40.0%
! GTTTAAGATCAGCAGCAGAA+GGG + chr2_2:19991696-19991715 None:intergenic 40.0%
! TGTTTAAGATCAGCAGCAGA+AGG + chr2_2:19991697-19991716 None:intergenic 40.0%
CGGATACTAATCATCGAAAC+CGG + chr2_2:19991822-19991841 None:intergenic 40.0%
CTTCATTATACCCACAGTCT+AGG + chr2_2:19991957-19991976 None:intergenic 40.0%
TGAATGAACATGGGTCTGTT+TGG - chr2_2:19992046-19992065 Msa0243140:CDS 40.0%
CGTTTGAGCTTATCTACTGT+TGG - chr2_2:19992077-19992096 Msa0243140:CDS 40.0%
TTGCTCCTAAGTGAAGTCAA+GGG - chr2_2:19992823-19992842 Msa0243140:intron 40.0%
GCGTAATGTTATCTTTGTGC+AGG - chr2_2:19993083-19993102 Msa0243140:intron 40.0%
!! AGCTTACTTGTTGGCCGTTT+TGG - chr2_2:19991657-19991676 Msa0243140:three_prime_UTR 45.0%
!! GCTTACTTGTTGGCCGTTTT+GGG - chr2_2:19991658-19991677 Msa0243140:three_prime_UTR 45.0%
GAACATGGGTCTGTTTGGAT+TGG - chr2_2:19992051-19992070 Msa0243140:CDS 45.0%
CAACTCAATGTTGCCATCCT+CGG + chr2_2:19992806-19992825 None:intergenic 45.0%
GTTGCTCCTAAGTGAAGTCA+AGG - chr2_2:19992822-19992841 Msa0243140:intron 45.0%
TCTTCCACTGGCAATAAGCT+CGG + chr2_2:19992860-19992879 None:intergenic 45.0%
TGCCAGTGGAAGAGAAAAGT+TGG - chr2_2:19992867-19992886 Msa0243140:intron 45.0%
! AGCCAACTTTTCTCTTCCAC+TGG + chr2_2:19992872-19992891 None:intergenic 45.0%
! AAGTTGGCTTCAGTTCCATC+CGG - chr2_2:19992883-19992902 Msa0243140:intron 45.0%
AGCAGCAAAGGAAGAGAAGA+AGG - chr2_2:19992969-19992988 Msa0243140:intron 45.0%
AGCAAAGGAAGAGAAGAAGG+TGG - chr2_2:19992972-19992991 Msa0243140:intron 45.0%
AGAGAAGAAGGTGGAAGAGA+AGG - chr2_2:19992981-19993000 Msa0243140:intron 45.0%
! TCTCTCTTTTCCTCCTCTAG+TGG - chr2_2:19993322-19993341 Msa0243140:intron 45.0%
CATGAATCAACCACTAGAGG+AGG + chr2_2:19993335-19993354 None:intergenic 45.0%
TTGACTGGTACACCAGATGT+AGG - chr2_2:19993457-19993476 Msa0243140:five_prime_UTR 45.0%
TGACTGGTACACCAGATGTA+GGG - chr2_2:19993458-19993477 Msa0243140:five_prime_UTR 45.0%
!! AGCTTACTTGTTGGCCGTTT+TGG - chr2_2:19991657-19991676 Msa0243140:three_prime_UTR 45.0%
!! GCTTACTTGTTGGCCGTTTT+GGG - chr2_2:19991658-19991677 Msa0243140:three_prime_UTR 45.0%
GAACATGGGTCTGTTTGGAT+TGG - chr2_2:19992051-19992070 Msa0243140:CDS 45.0%
CAACTCAATGTTGCCATCCT+CGG + chr2_2:19992806-19992825 None:intergenic 45.0%
GTTGCTCCTAAGTGAAGTCA+AGG - chr2_2:19992822-19992841 Msa0243140:intron 45.0%
TCTTCCACTGGCAATAAGCT+CGG + chr2_2:19992860-19992879 None:intergenic 45.0%
TGCCAGTGGAAGAGAAAAGT+TGG - chr2_2:19992867-19992886 Msa0243140:intron 45.0%
! AGCCAACTTTTCTCTTCCAC+TGG + chr2_2:19992872-19992891 None:intergenic 45.0%
! AAGTTGGCTTCAGTTCCATC+CGG - chr2_2:19992883-19992902 Msa0243140:intron 45.0%
AGCAGCAAAGGAAGAGAAGA+AGG - chr2_2:19992969-19992988 Msa0243140:intron 45.0%
AGCAAAGGAAGAGAAGAAGG+TGG - chr2_2:19992972-19992991 Msa0243140:intron 45.0%
AGAGAAGAAGGTGGAAGAGA+AGG - chr2_2:19992981-19993000 Msa0243140:intron 45.0%
! TCTCTCTTTTCCTCCTCTAG+TGG - chr2_2:19993322-19993341 Msa0243140:intron 45.0%
CATGAATCAACCACTAGAGG+AGG + chr2_2:19993335-19993354 None:intergenic 45.0%
! GAGATCTCGACTTGACGGTA+GGG + chr2_2:19991506-19991525 None:intergenic 50.0%
! TGAGATCTCGACTTGACGGT+AGG + chr2_2:19991507-19991526 None:intergenic 50.0%
! CGGATGAGATCTCGACTTGA+CGG + chr2_2:19991511-19991530 None:intergenic 50.0%
!! TACTTGTTGGCCGTTTTGGG+AGG - chr2_2:19991661-19991680 Msa0243140:three_prime_UTR 50.0%
!! GGATTGGTTGCCTCCCAAAA+CGG + chr2_2:19991674-19991693 None:intergenic 50.0%
CCTTGCCCTTGACTTCACTT+AGG + chr2_2:19992831-19992850 None:intergenic 50.0%
CCTAAGTGAAGTCAAGGGCA+AGG - chr2_2:19992828-19992847 Msa0243140:intron 50.0%
TTTGCCGAGCTTATTGCCAG+TGG - chr2_2:19992853-19992872 Msa0243140:intron 50.0%
! TCTTCCTTTGCTGCTTCAGC+AGG + chr2_2:19992964-19992983 None:intergenic 50.0%
! GAGATCTCGACTTGACGGTA+GGG + chr2_2:19991506-19991525 None:intergenic 50.0%
! TGAGATCTCGACTTGACGGT+AGG + chr2_2:19991507-19991526 None:intergenic 50.0%
! CGGATGAGATCTCGACTTGA+CGG + chr2_2:19991511-19991530 None:intergenic 50.0%
!! TACTTGTTGGCCGTTTTGGG+AGG - chr2_2:19991661-19991680 Msa0243140:three_prime_UTR 50.0%
!! GGATTGGTTGCCTCCCAAAA+CGG + chr2_2:19991674-19991693 None:intergenic 50.0%
CCTTGCCCTTGACTTCACTT+AGG + chr2_2:19992831-19992850 None:intergenic 50.0%
CCTAAGTGAAGTCAAGGGCA+AGG - chr2_2:19992828-19992847 Msa0243140:intron 50.0%
TTTGCCGAGCTTATTGCCAG+TGG - chr2_2:19992853-19992872 Msa0243140:intron 50.0%
! TCTTCCTTTGCTGCTTCAGC+AGG + chr2_2:19992964-19992983 None:intergenic 50.0%
GACGGTAGGGTTTCACCAGA+GGG + chr2_2:19991493-19991512 None:intergenic 55.0%
TGACGGTAGGGTTTCACCAG+AGG + chr2_2:19991494-19991513 None:intergenic 55.0%
GATCAGCAGCAGAAGGGGAT+TGG + chr2_2:19991690-19991709 None:intergenic 55.0%
TGTAGTTGGAGTTGAGGCCG+AGG - chr2_2:19992786-19992805 Msa0243140:intron 55.0%
TTGGCTTCAGTTCCATCCGG+TGG - chr2_2:19992886-19992905 Msa0243140:intron 55.0%
GTTGCTGTGTCTGCTGCAAC+TGG - chr2_2:19992916-19992935 Msa0243140:intron 55.0%
TGCACCTGCTGAAGCAGCAA+AGG - chr2_2:19992957-19992976 Msa0243140:intron 55.0%
GACGGTAGGGTTTCACCAGA+GGG + chr2_2:19991493-19991512 None:intergenic 55.0%
TGACGGTAGGGTTTCACCAG+AGG + chr2_2:19991494-19991513 None:intergenic 55.0%
GATCAGCAGCAGAAGGGGAT+TGG + chr2_2:19991690-19991709 None:intergenic 55.0%
TGTAGTTGGAGTTGAGGCCG+AGG - chr2_2:19992786-19992805 Msa0243140:intron 55.0%
TTGGCTTCAGTTCCATCCGG+TGG - chr2_2:19992886-19992905 Msa0243140:intron 55.0%
GTTGCTGTGTCTGCTGCAAC+TGG - chr2_2:19992916-19992935 Msa0243140:intron 55.0%
TGCACCTGCTGAAGCAGCAA+AGG - chr2_2:19992957-19992976 Msa0243140:intron 55.0%
GTTGGAGTTGAGGCCGAGGA+TGG - chr2_2:19992790-19992809 Msa0243140:intron 60.0%
GCTTCAGTTCCATCCGGTGG+TGG - chr2_2:19992889-19992908 Msa0243140:intron 60.0%
CACAGCAACAGCACCACCAC+CGG + chr2_2:19992905-19992924 None:intergenic 60.0%
GCTGTGTCTGCTGCAACTGG+AGG - chr2_2:19992919-19992938 Msa0243140:intron 60.0%
GTGTCTGCTGCAACTGGAGG+AGG - chr2_2:19992922-19992941 Msa0243140:intron 60.0%
GTTGGAGTTGAGGCCGAGGA+TGG - chr2_2:19992790-19992809 Msa0243140:intron 60.0%
GCTTCAGTTCCATCCGGTGG+TGG - chr2_2:19992889-19992908 Msa0243140:intron 60.0%
CACAGCAACAGCACCACCAC+CGG + chr2_2:19992905-19992924 None:intergenic 60.0%
GCTGTGTCTGCTGCAACTGG+AGG - chr2_2:19992919-19992938 Msa0243140:intron 60.0%
GTGTCTGCTGCAACTGGAGG+AGG - chr2_2:19992922-19992941 Msa0243140:intron 60.0%
GCAACAGCACCACCACCGGA+TGG + chr2_2:19992901-19992920 None:intergenic 65.0%
GCAACAGCACCACCACCGGA+TGG + chr2_2:19992901-19992920 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 19991476 19993479 19991476 ID=Msa0243140;Name=Msa0243140
chr2_2 mRNA 19991476 19993479 19991476 ID=Msa0243140-mRNA-1;Parent=Msa0243140;Name=Msa0243140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=70|1|1|1|0.66|0.5|4|348|113
chr2_2 exon 19991476 19991850 19991476 ID=Msa0243140-mRNA-1:exon:8063;Parent=Msa0243140-mRNA-1
chr2_2 exon 19991938 19992164 19991938 ID=Msa0243140-mRNA-1:exon:8062;Parent=Msa0243140-mRNA-1
chr2_2 exon 19993225 19993314 19993225 ID=Msa0243140-mRNA-1:exon:8061;Parent=Msa0243140-mRNA-1
chr2_2 exon 19993412 19993479 19993412 ID=Msa0243140-mRNA-1:exon:8060;Parent=Msa0243140-mRNA-1
chr2_2 five_prime_UTR 19993412 19993479 19993412 ID=Msa0243140-mRNA-1:five_prime_utr;Parent=Msa0243140-mRNA-1
chr2_2 five_prime_UTR 19993313 19993314 19993313 ID=Msa0243140-mRNA-1:five_prime_utr;Parent=Msa0243140-mRNA-1
chr2_2 CDS 19993225 19993312 19993225 ID=Msa0243140-mRNA-1:cds;Parent=Msa0243140-mRNA-1
chr2_2 CDS 19991938 19992164 19991938 ID=Msa0243140-mRNA-1:cds;Parent=Msa0243140-mRNA-1
chr2_2 CDS 19991824 19991850 19991824 ID=Msa0243140-mRNA-1:cds;Parent=Msa0243140-mRNA-1
chr2_2 three_prime_UTR 19991476 19991823 19991476 ID=Msa0243140-mRNA-1:three_prime_utr;Parent=Msa0243140-mRNA-1
Gene Sequence

>Msa0243140

ATGAAGATTATCGCAGCTTACTTGTTGGCCGTTTTGGGAGGCAACCAATCCCCTTCTGCTGCTGATCTTAAACAAATTCTTGGATCAGTTGGAGTTGAGGCCGAGGATGGCAACATTGAGTTGCTCCTAAGTGAAGTCAAGGGCAAGGACTTTGCCGAGCTTATTGCCAGTGGAAGAGAAAAGTTGGCTTCAGTTCCATCCGGTGGTGGTGCTGTTGCTGTGTCTGCTGCAACTGGAGGAGGTGCTGCAGCTGCTGCACCTGCTGAAGCAGCAAAGGAAGAGAAGAAGGTGGAAGAGAAGGAAGAATCCGATGATGATATGGGATTCAGCTTATTTGATTAA
Protein sequence

>Msa0243140

MKIIAAYLLAVLGGNQSPSAADLKQILGSVGVEAEDGNIELLLSEVKGKDFAELIASGREKLASVPSGGGAVAVSAATGGGAAAAAPAEAAKEEKKVEEKEESDDDMGFSLFD*