AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0366430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0366430 MtrunA17_Chr3g0107461 86.335 161 22 0 1 161 1 161 8.53e-101 286
Msa0366430 MtrunA17_Chr4g0031421 77.444 133 30 0 29 161 1 133 2.93e-72 213
Msa0366430 MtrunA17_Chr3g0105791 76.692 133 31 0 29 161 1 133 2.03e-70 209
Msa0366430 MtrunA17_Chr3g0107381 54.037 161 73 1 1 161 1 160 8.13e-57 177
Msa0366430 MtrunA17_Chr3g0110131 49.045 157 79 1 1 157 1 156 3.54e-42 140
Msa0366430 MtrunA17_Chr2g0283941 42.500 160 92 0 1 160 1 160 4.61e-41 137
Msa0366430 MtrunA17_Chr2g0296861 47.097 155 81 1 1 155 1 154 4.31e-39 130
Msa0366430 MtrunA17_Chr4g0014321 38.312 154 95 0 1 154 1 154 1.19e-37 128
Msa0366430 MtrunA17_Chr8g0351751 42.038 157 89 1 1 155 1 157 1.36e-37 129
Msa0366430 MtrunA17_Chr5g0432691 39.610 154 92 1 1 154 1 153 7.13e-37 124
Msa0366430 MtrunA17_Chr4g0014031 44.516 155 86 0 1 155 1 155 1.42e-36 125
Msa0366430 MtrunA17_Chr4g0014131 44.099 161 90 0 1 161 1 161 1.53e-36 125
Msa0366430 MtrunA17_Chr3g0107331 40.645 155 91 1 3 157 2 155 1.82e-35 120
Msa0366430 MtrunA17_Chr3g0106421 44.697 132 73 0 29 160 1 132 1.89e-34 120
Msa0366430 MtrunA17_Chr4g0014121 40.645 155 92 0 1 155 29 183 2.39e-33 116
Msa0366430 MtrunA17_Chr3g0106501 47.287 129 68 0 29 157 1 129 3.50e-33 115
Msa0366430 MtrunA17_Chr4g0013041 36.364 154 96 1 1 154 1 152 8.42e-32 113
Msa0366430 MtrunA17_Chr3g0107351 37.419 155 96 1 3 157 2 155 1.22e-31 111
Msa0366430 MtrunA17_Chr2g0296831 47.541 122 64 0 1 122 1 122 3.18e-31 108
Msa0366430 MtrunA17_Chr5g0420351 39.610 154 91 2 1 154 1 152 4.49e-31 109
Msa0366430 MtrunA17_Chr3g0107341 37.419 155 96 1 3 157 2 155 1.01e-29 106
Msa0366430 MtrunA17_Chr3g0107361 36.774 155 97 1 3 157 2 155 5.64e-29 104
Msa0366430 MtrunA17_Chr1g0188681 38.961 154 92 2 1 154 1 152 1.09e-28 103
Msa0366430 MtrunA17_Chr1g0197461 34.868 152 97 2 3 154 2 151 6.93e-26 96.3
Msa0366430 MtrunA17_Chr4g0012761 36.943 157 97 2 1 157 1 155 6.54e-25 94.0
Msa0366430 MtrunA17_Chr1g0188661 37.419 155 93 3 1 154 1 152 1.30e-24 93.2
Msa0366430 MtrunA17_Chr1g0197261 32.903 155 100 3 1 154 1 152 5.33e-24 91.7
Msa0366430 MtrunA17_Chr1g0193311 37.419 155 93 3 1 154 1 152 3.99e-23 89.4
Msa0366430 MtrunA17_Chr7g0218151 35.032 157 100 2 1 157 1 155 4.25e-23 89.4
Msa0366430 MtrunA17_Chr1g0188651 35.404 161 100 2 1 161 1 157 7.70e-23 88.6
Msa0366430 MtrunA17_Chr1g0197321 32.051 156 101 3 1 154 1 153 5.10e-22 86.7
Msa0366430 MtrunA17_Chr4g0008701 30.137 146 94 4 16 158 19 159 2.43e-15 72.4
Msa0366430 MtrunA17_Chr7g0267601 27.673 159 107 4 3 158 6 159 3.57e-15 72.0
Msa0366430 MtrunA17_Chr3g0128721 27.344 128 87 2 16 139 19 144 3.21e-12 63.5
Msa0366430 MtrunA17_Chr6g0449851 27.500 160 101 5 1 154 1 151 4.59e-12 62.4
Msa0366430 MtrunA17_Chr1g0212151 26.946 167 106 6 1 161 1 157 8.58e-12 62.0
Msa0366430 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 1.03e-11 61.6
Msa0366430 MtrunA17_Chr1g0212471 28.750 160 98 6 1 154 1 150 5.77e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0366430 AT5G48670.1 48.077 156 78 2 1 155 1 154 9.02e-37 129
Msa0366430 AT1G65300.1 40.127 157 92 2 5 161 4 158 3.59e-31 113
Msa0366430 AT3G05860.2 41.026 156 79 3 1 150 1 149 1.23e-30 110
Msa0366430 AT1G65330.1 40.541 148 87 1 3 150 2 148 4.90e-30 110
Msa0366430 AT3G05860.3 41.026 156 79 3 1 150 1 149 5.59e-30 109
Msa0366430 AT3G05860.1 41.026 156 79 3 1 150 1 149 7.33e-30 109
Msa0366430 AT5G27810.1 52.809 89 42 0 29 117 1 89 1.58e-27 99.8
Msa0366430 AT5G26650.1 34.641 153 99 1 5 157 3 154 1.35e-23 95.1
Msa0366430 AT5G27960.1 43.396 106 60 0 5 110 3 108 8.35e-23 92.4
Msa0366430 AT1G31630.1 35.928 167 87 6 3 161 2 156 1.32e-22 92.0
Msa0366430 AT1G31640.1 39.024 164 85 5 3 160 2 156 1.48e-22 93.2
Msa0366430 AT5G26630.1 35.065 154 99 1 1 154 1 153 3.15e-22 89.0
Msa0366430 AT2G28700.1 35.065 154 91 3 1 150 1 149 1.90e-21 89.0
Msa0366430 AT1G22590.2 39.450 109 66 0 1 109 1 109 2.30e-21 85.5
Msa0366430 AT5G06500.1 36.364 110 66 1 1 110 1 106 2.72e-18 79.0
Msa0366430 AT5G26580.1 37.624 101 49 1 1 101 1 87 4.56e-14 68.6
Msa0366430 AT2G40210.1 33.663 101 67 0 1 101 1 101 4.66e-13 65.9
Msa0366430 AT5G26865.1 41.667 72 41 1 24 94 2 73 2.09e-11 58.5

Find 35 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTCCTTTC+TGG 0.057843 3_1:+61323925 None:intergenic
ATTCATACCACAGAATTTAA+TGG 0.196701 3_1:-61323887 Msa0366430:CDS
CAGGTTTGGCCATCAGATTC+AGG 0.224706 3_1:-61324082 Msa0366430:CDS
CTGAATGTTATCCTCCTCTT+CGG 0.350265 3_1:+61324402 None:intergenic
TATTCTTGAGGCAAAGTATT+GGG 0.351669 3_1:-61323985 Msa0366430:CDS
GTTCAAGAGTTTGCATGAAC+TGG 0.380322 3_1:-61324038 Msa0366430:CDS
GATGAAATTAGCACTCTTTG+TGG 0.382102 3_1:-61324151 Msa0366430:CDS
CTGAATCTGATGGCCAAACC+TGG 0.394792 3_1:+61324083 None:intergenic
TGAATGTTATCCTCCTCTTC+GGG 0.395416 3_1:+61324403 None:intergenic
TTTGTTTCATCAACTGCTCA+TGG 0.410143 3_1:+61323957 None:intergenic
AATGAAATGATGAATGATAT+TGG 0.442815 3_1:+61323907 None:intergenic
AGGTTTGGCCATCAGATTCA+GGG 0.444978 3_1:-61324081 Msa0366430:CDS
ACAATCCTCAAGCCCAGGTT+TGG 0.451431 3_1:-61324096 Msa0366430:CDS
GGGTTAGAAGTGTGATATCC+AGG 0.506627 3_1:-61324060 Msa0366430:CDS
CACTATCATGGCTAGAGGTA+AGG 0.508320 3_1:-61324451 None:intergenic
GTATTCTTGAGGCAAAGTAT+TGG 0.524622 3_1:-61323986 Msa0366430:CDS
GGAGGATAACATTCAGAAAG+AGG 0.525464 3_1:-61324395 Msa0366430:CDS
ACTCTTTGTGGAATAGAAGC+TGG 0.543874 3_1:-61324139 Msa0366430:CDS
TAAATATCCATTAAATTCTG+TGG 0.554522 3_1:+61323880 None:intergenic
GATGGCCAAACCTGGGCTTG+AGG 0.558655 3_1:+61324091 None:intergenic
GCAAAACAATCCTCAAGCCC+AGG 0.573782 3_1:-61324101 Msa0366430:CDS
ACAAAGGAAGGAAACCAGAA+AGG 0.579534 3_1:-61323939 Msa0366430:CDS
TTCATGCAAACTCTTGAACC+TGG 0.581655 3_1:+61324042 None:intergenic
CAAATTCTCATCAATAAAAG+CGG 0.585206 3_1:+61323829 None:intergenic
TGAATCTGATGGCCAAACCT+GGG 0.592740 3_1:+61324084 None:intergenic
CTTCTAACCCCTGAATCTGA+TGG 0.608216 3_1:+61324073 None:intergenic
AAGGAAGGAAACCAGAAAGG+AGG 0.615022 3_1:-61323936 Msa0366430:CDS
ACATAACATGTGACCCGAAG+AGG 0.618667 3_1:-61324416 Msa0366430:CDS
ATGAGCAGTTGATGAAACAA+AGG 0.621497 3_1:-61323955 Msa0366430:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.621539 3_1:-61324080 Msa0366430:CDS
GCAGTTGATGAAACAAAGGA+AGG 0.627441 3_1:-61323951 Msa0366430:CDS
TATTGATGAGAATTTGAGGG+AGG 0.643852 3_1:-61323822 Msa0366430:CDS
ATCCTCACTATCATGGCTAG+AGG 0.659018 3_1:-61324456 None:intergenic
TAACATGTGACCCGAAGAGG+AGG 0.659370 3_1:-61324413 Msa0366430:CDS
TGGATCAAGAGGTATTCTTG+AGG 0.709854 3_1:-61323997 Msa0366430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATGGATTTTTTTTTATT+AGG - chr3_1:61324038-61324057 Msa0366430:CDS 10.0%
!!! TTATGGATTTTTTTTTATTA+GGG - chr3_1:61324039-61324058 Msa0366430:CDS 10.0%
!!! TAAATTTGGAATTTTACTAT+AGG - chr3_1:61324005-61324024 Msa0366430:CDS 15.0%
!! TATAGGTTATATATTGTTTA+TGG - chr3_1:61324022-61324041 Msa0366430:CDS 15.0%
!! AATGAAATGATGAATGATAT+TGG + chr3_1:61324324-61324343 None:intergenic 20.0%
!! TAAATATCCATTAAATTCTG+TGG + chr3_1:61324351-61324370 None:intergenic 20.0%
!!! TTTTATTGATGAGAATTTGA+GGG - chr3_1:61324403-61324422 Msa0366430:CDS 20.0%
! ATTCATACCACAGAATTTAA+TGG - chr3_1:61324341-61324360 Msa0366430:intron 25.0%
! CAAATTCTCATCAATAAAAG+CGG + chr3_1:61324402-61324421 None:intergenic 25.0%
!! CTTTTATTGATGAGAATTTG+AGG - chr3_1:61324402-61324421 Msa0366430:CDS 25.0%
ACATTCAGAAAGAGGAAAAA+TGG - chr3_1:61323841-61323860 Msa0366430:CDS 30.0%
TATGTATGATATGCAAAGAG+AGG + chr3_1:61323877-61323896 None:intergenic 30.0%
ACTGGAACAAAGTAAAAAGA+TGG - chr3_1:61324208-61324227 Msa0366430:intron 30.0%
! TATTCTTGAGGCAAAGTATT+GGG - chr3_1:61324243-61324262 Msa0366430:intron 30.0%
GATGAAATTAGCACTCTTTG+TGG - chr3_1:61324077-61324096 Msa0366430:CDS 35.0%
GGAACAAAGTAAAAAGATGG+TGG - chr3_1:61324211-61324230 Msa0366430:intron 35.0%
!! TAAAAAGATGGTGGATCAAG+AGG - chr3_1:61324220-61324239 Msa0366430:intron 35.0%
GTATTCTTGAGGCAAAGTAT+TGG - chr3_1:61324242-61324261 Msa0366430:intron 35.0%
TTTGTTTCATCAACTGCTCA+TGG + chr3_1:61324274-61324293 None:intergenic 35.0%
!! ATGAGCAGTTGATGAAACAA+AGG - chr3_1:61324273-61324292 Msa0366430:intron 35.0%
TATTGATGAGAATTTGAGGG+AGG - chr3_1:61324406-61324425 Msa0366430:CDS 35.0%
TGAATGTTATCCTCCTCTTC+GGG + chr3_1:61323828-61323847 None:intergenic 40.0%
CTGAATGTTATCCTCCTCTT+CGG + chr3_1:61323829-61323848 None:intergenic 40.0%
GGAGGATAACATTCAGAAAG+AGG - chr3_1:61323833-61323852 Msa0366430:CDS 40.0%
ACTCTTTGTGGAATAGAAGC+TGG - chr3_1:61324089-61324108 Msa0366430:CDS 40.0%
TTCATGCAAACTCTTGAACC+TGG + chr3_1:61324189-61324208 None:intergenic 40.0%
GTTCAAGAGTTTGCATGAAC+TGG - chr3_1:61324190-61324209 Msa0366430:intron 40.0%
!! TGGATCAAGAGGTATTCTTG+AGG - chr3_1:61324231-61324250 Msa0366430:intron 40.0%
!! GCAGTTGATGAAACAAAGGA+AGG - chr3_1:61324277-61324296 Msa0366430:intron 40.0%
ACAAAGGAAGGAAACCAGAA+AGG - chr3_1:61324289-61324308 Msa0366430:intron 40.0%
ATTGGTCATTTCCTCCTTTC+TGG + chr3_1:61324306-61324325 None:intergenic 40.0%
ACATAACATGTGACCCGAAG+AGG - chr3_1:61323812-61323831 Msa0366430:CDS 45.0%
TGAATCTGATGGCCAAACCT+GGG + chr3_1:61324147-61324166 None:intergenic 45.0%
AGGTTTGGCCATCAGATTCA+GGG - chr3_1:61324147-61324166 Msa0366430:CDS 45.0%
CTTCTAACCCCTGAATCTGA+TGG + chr3_1:61324158-61324177 None:intergenic 45.0%
! GGGTTAGAAGTGTGATATCC+AGG - chr3_1:61324168-61324187 Msa0366430:CDS 45.0%
AAGGAAGGAAACCAGAAAGG+AGG - chr3_1:61324292-61324311 Msa0366430:intron 45.0%
!!! TAACTTTTATTTAATAAATT+TGG - chr3_1:61323991-61324010 Msa0366430:CDS 5.0%
TAACATGTGACCCGAAGAGG+AGG - chr3_1:61323815-61323834 Msa0366430:CDS 50.0%
GCAAAACAATCCTCAAGCCC+AGG - chr3_1:61324127-61324146 Msa0366430:CDS 50.0%
ACAATCCTCAAGCCCAGGTT+TGG - chr3_1:61324132-61324151 Msa0366430:CDS 50.0%
CTGAATCTGATGGCCAAACC+TGG + chr3_1:61324148-61324167 None:intergenic 50.0%
CAGGTTTGGCCATCAGATTC+AGG - chr3_1:61324146-61324165 Msa0366430:CDS 50.0%
GGTTTGGCCATCAGATTCAG+GGG - chr3_1:61324148-61324167 Msa0366430:CDS 50.0%
GATGGCCAAACCTGGGCTTG+AGG + chr3_1:61324140-61324159 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 61323784 61324466 61323784 ID=Msa0366430;Name=Msa0366430
chr3_1 mRNA 61323784 61324466 61323784 ID=Msa0366430-mRNA-1;Parent=Msa0366430;Name=Msa0366430-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|161
chr3_1 exon 61324388 61324466 61324388 ID=Msa0366430-mRNA-1:exon:11882;Parent=Msa0366430-mRNA-1
chr3_1 exon 61323784 61324190 61323784 ID=Msa0366430-mRNA-1:exon:11881;Parent=Msa0366430-mRNA-1
chr3_1 CDS 61324388 61324466 61324388 ID=Msa0366430-mRNA-1:cds;Parent=Msa0366430-mRNA-1
chr3_1 CDS 61323784 61324190 61323784 ID=Msa0366430-mRNA-1:cds;Parent=Msa0366430-mRNA-1
Gene Sequence

>Msa0366430

ATGGCTAGAGGTAAGGTGAAACTAGCATACATAACATGTGACCCGAAGAGGAGGATAACATTCAGAAAGAGGAAAAATGGGATTATGAAGAAGATTGATGAAATTAGCACTCTTTGTGGAATAGAAGCTGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAAGAGTTTGCATGAACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGTATTGGGAAAGTCCATGAGCAGTTGATGAAACAAAGGAAGGAAACCAGAAAGGAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACATGATCTTAATGATTTTTCCGCTTTTATTGATGAGAATTTGAGGGAGGTCGATCAAAAGATGAAACGAATGCTAATCGAAGATTGA

Protein sequence

>Msa0366430

MARGKVKLAYITCDPKRRITFRKRKNGIMKKIDEISTLCGIEAGAIIYEQNNPQAQVWPSDSGVRSVISRFKSLHELEQSKKMVDQEVFLRQSIGKVHEQLMKQRKETRKEEMTNIIHHFIHTTEFNGYLMSKHDLNDFSAFIDENLREVDQKMKRMLIED*