AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414410 MtrunA17_Chr3g0107461 90.062 161 16 0 1 161 1 161 1.30e-106 301
Msa0414410 MtrunA17_Chr4g0031421 78.947 133 28 0 29 161 1 133 6.90e-74 218
Msa0414410 MtrunA17_Chr3g0105791 78.195 133 29 0 29 161 1 133 1.59e-72 214
Msa0414410 MtrunA17_Chr3g0107381 55.195 154 68 1 1 154 1 153 5.55e-57 177
Msa0414410 MtrunA17_Chr3g0110131 50.323 155 76 1 1 155 1 154 7.54e-43 142
Msa0414410 MtrunA17_Chr2g0283941 43.590 156 88 0 5 160 5 160 9.62e-42 139
Msa0414410 MtrunA17_Chr2g0296861 48.387 155 79 1 1 155 1 154 4.76e-41 135
Msa0414410 MtrunA17_Chr5g0432691 40.260 154 91 1 1 154 1 153 4.36e-38 127
Msa0414410 MtrunA17_Chr8g0351751 41.509 159 91 1 1 157 1 159 4.17e-37 127
Msa0414410 MtrunA17_Chr4g0014321 38.312 154 95 0 1 154 1 154 1.03e-36 126
Msa0414410 MtrunA17_Chr3g0107331 41.060 151 88 1 3 153 2 151 2.26e-34 118
Msa0414410 MtrunA17_Chr4g0014031 43.590 156 88 0 1 156 1 156 4.59e-34 119
Msa0414410 MtrunA17_Chr4g0014131 43.478 161 91 0 1 161 1 161 4.62e-34 119
Msa0414410 MtrunA17_Chr3g0106421 44.697 132 73 0 29 160 1 132 8.64e-34 118
Msa0414410 MtrunA17_Chr2g0296831 51.282 117 57 0 1 117 1 117 1.41e-33 115
Msa0414410 MtrunA17_Chr3g0106501 45.736 129 70 0 29 157 1 129 5.62e-32 112
Msa0414410 MtrunA17_Chr4g0014121 39.744 156 94 0 1 156 29 184 3.97e-31 110
Msa0414410 MtrunA17_Chr4g0013041 37.791 172 86 4 1 157 1 166 4.87e-31 111
Msa0414410 MtrunA17_Chr5g0420351 38.854 157 94 2 1 157 1 155 6.21e-31 109
Msa0414410 MtrunA17_Chr3g0107351 36.774 155 97 1 3 157 2 155 2.56e-30 107
Msa0414410 MtrunA17_Chr1g0188681 40.000 160 88 4 1 157 1 155 9.90e-29 103
Msa0414410 MtrunA17_Chr3g0107341 37.500 152 94 1 3 154 2 152 2.73e-28 102
Msa0414410 MtrunA17_Chr3g0107361 37.500 152 94 1 3 154 2 152 3.21e-28 102
Msa0414410 MtrunA17_Chr1g0197461 34.839 155 99 2 3 157 2 154 1.64e-27 100
Msa0414410 MtrunA17_Chr1g0188661 38.608 158 93 3 1 157 1 155 1.75e-26 98.2
Msa0414410 MtrunA17_Chr4g0012761 38.854 157 94 2 1 157 1 155 2.34e-26 97.8
Msa0414410 MtrunA17_Chr7g0218151 36.943 157 97 2 1 157 1 155 9.13e-25 93.6
Msa0414410 MtrunA17_Chr1g0188651 37.267 161 97 2 1 161 1 157 1.29e-24 93.2
Msa0414410 MtrunA17_Chr1g0197261 32.911 158 102 3 1 157 1 155 3.70e-24 92.0
Msa0414410 MtrunA17_Chr1g0197321 33.333 159 101 3 1 157 1 156 9.78e-24 90.9
Msa0414410 MtrunA17_Chr1g0193311 36.709 158 96 3 1 157 1 155 1.32e-23 90.5
Msa0414410 MtrunA17_Chr4g0008701 28.859 149 99 3 3 148 6 150 4.63e-15 71.6
Msa0414410 MtrunA17_Chr7g0267601 28.947 114 81 0 5 118 8 121 1.88e-13 67.0
Msa0414410 MtrunA17_Chr3g0128721 25.490 153 105 2 5 153 8 155 2.94e-12 63.5
Msa0414410 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 6.25e-12 62.4
Msa0414410 MtrunA17_Chr1g0212151 28.448 116 76 3 1 110 1 115 1.38e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414410 AT5G48670.1 50.000 156 75 2 1 155 1 154 6.69e-39 134
Msa0414410 AT3G05860.2 53.535 99 46 0 1 99 1 99 8.61e-31 110
Msa0414410 AT1G65330.1 41.892 148 85 1 3 150 2 148 1.30e-30 112
Msa0414410 AT3G05860.3 53.535 99 46 0 1 99 1 99 1.78e-30 111
Msa0414410 AT3G05860.1 53.535 99 46 0 1 99 1 99 2.84e-30 110
Msa0414410 AT1G65300.1 40.127 157 92 2 5 161 4 158 4.05e-30 110
Msa0414410 AT5G27810.1 53.933 89 41 0 29 117 1 89 4.04e-29 103
Msa0414410 AT5G26650.1 36.601 153 96 1 5 157 3 154 8.21e-26 101
Msa0414410 AT5G27960.1 35.294 153 98 1 5 157 3 154 1.64e-24 97.1
Msa0414410 AT1G31630.1 36.076 158 87 4 3 154 2 151 7.49e-24 95.5
Msa0414410 AT1G31640.1 39.623 159 82 4 3 155 2 152 1.28e-23 96.3
Msa0414410 AT1G22590.2 33.750 160 89 2 1 157 1 146 1.54e-23 90.9
Msa0414410 AT2G28700.1 32.667 150 100 1 1 150 1 149 9.77e-22 89.7
Msa0414410 AT5G26630.1 33.548 155 102 1 1 155 1 154 4.05e-21 85.9
Msa0414410 AT5G06500.1 33.333 147 88 3 1 147 1 137 3.82e-19 81.3
Msa0414410 AT2G40210.1 34.653 101 66 0 1 101 1 101 2.78e-14 69.3
Msa0414410 AT5G26580.1 37.624 101 49 1 1 101 1 87 8.81e-14 67.8
Msa0414410 AT3G57230.1 29.012 162 100 4 1 161 1 148 3.51e-12 62.8
Msa0414410 AT3G57230.4 29.012 162 100 4 1 161 1 148 3.51e-12 62.8

Find 36 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTGCTTTC+TGG 0.066665 3_2:-59915203 None:intergenic
ATTCATACCACAGAATTTAA+TGG 0.196701 3_2:+59915241 Msa0414410:CDS
CAGGTTTGGCCATCAGATTC+AGG 0.224706 3_2:+59915046 Msa0414410:CDS
CCTGGGCTTGAGGATTGTTT+TGG 0.292217 3_2:-59915027 None:intergenic
GATGAAATTAGCACTCTTTG+TGG 0.382102 3_2:+59914977 Msa0414410:CDS
GTTCAGGAGTTTGCCTGGAC+TGG 0.382826 3_2:+59915090 Msa0414410:CDS
CTGAATCTGATGGCCAAACC+TGG 0.394792 3_2:-59915045 None:intergenic
GAAGTGTGATATCCAGGTTC+AGG 0.406864 3_2:+59915074 Msa0414410:CDS
AATGAAATGATGAATGATAT+TGG 0.442815 3_2:-59915221 None:intergenic
AGGTTTGGCCATCAGATTCA+GGG 0.444978 3_2:+59915047 Msa0414410:CDS
TCCAGGTTCAGGAGTTTGCC+TGG 0.447666 3_2:+59915085 Msa0414410:CDS
ACAATCCTCAAGCCCAGGTT+TGG 0.451431 3_2:+59915032 Msa0414410:CDS
TATTCTTGAGGCAAAGCATT+GGG 0.452810 3_2:+59915143 Msa0414410:CDS
GGGTTAGAAGTGTGATATCC+AGG 0.506627 3_2:+59915068 Msa0414410:CDS
TCCAGGCAAACTCCTGAACC+TGG 0.517673 3_2:-59915086 None:intergenic
AAGGAAGGAAACCAGAAAGC+AGG 0.520416 3_2:+59915192 Msa0414410:CDS
CAAATTCTCAGCAATAAAAG+AGG 0.520681 3_2:-59915299 None:intergenic
GGAGGATGACATTCAGAAAG+AGG 0.520726 3_2:+59914737 Msa0414410:CDS
ACTCTTTGTGGAATAGAAGC+TGG 0.543874 3_2:+59914989 Msa0414410:CDS
TAAATATCCATTAAATTCTG+TGG 0.554522 3_2:-59915248 None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG 0.555152 3_2:+59915142 Msa0414410:CDS
GATGGCCAAACCTGGGCTTG+AGG 0.558655 3_2:-59915037 None:intergenic
CACTATCATGGCTAGAGGGA+AGG 0.570504 3_2:+59914681 None:intergenic
CCAAAACAATCCTCAAGCCC+AGG 0.591446 3_2:+59915027 Msa0414410:CDS
TGAATCTGATGGCCAAACCT+GGG 0.592740 3_2:-59915044 None:intergenic
TCCTCACTATCATGGCTAGA+GGG 0.594549 3_2:+59914677 None:intergenic
GAGGTCGATCAAAAGATGAA+AGG 0.605953 3_2:+59915325 Msa0414410:CDS
CTTCTAACCCCTGAATCTGA+TGG 0.608216 3_2:-59915055 None:intergenic
ATGAGCAGTTGATGAAACAA+AGG 0.608842 3_2:+59915173 Msa0414410:CDS
ACATAACATGTGACTCGAAG+AGG 0.616371 3_2:+59914716 Msa0414410:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.621539 3_2:+59915048 Msa0414410:CDS
GCAGTTGATGAAACAAAGGA+AGG 0.627441 3_2:+59915177 Msa0414410:CDS
ATCCTCACTATCATGGCTAG+AGG 0.627794 3_2:+59914676 None:intergenic
TATTGCTGAGAATTTGAGGG+AGG 0.639350 3_2:+59915306 Msa0414410:CDS
TAACATGTGACTCGAAGAGG+AGG 0.651743 3_2:+59914719 Msa0414410:CDS
TGGATCAAGAGGTATTCTTG+AGG 0.709854 3_2:+59915131 Msa0414410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTAGAATTTTACTAT+AGG + chr3_2:59914902-59914921 Msa0414410:intron 10.0%
!!! ATGGATATATTTTTTTTAAT+AGG + chr3_2:59914938-59914957 Msa0414410:intron 10.0%
!! TATAGGTTATATATTGTTTA+TGG + chr3_2:59914919-59914938 Msa0414410:intron 15.0%
!! AATGAAATGATGAATGATAT+TGG - chr3_2:59915224-59915243 None:intergenic 20.0%
!! TAAATATCCATTAAATTCTG+TGG - chr3_2:59915251-59915270 None:intergenic 20.0%
! ATTCATACCACAGAATTTAA+TGG + chr3_2:59915241-59915260 Msa0414410:CDS 25.0%
!! TTTTATTGCTGAGAATTTGA+GGG + chr3_2:59915303-59915322 Msa0414410:CDS 25.0%
ACATTCAGAAAGAGGAAAAA+TGG + chr3_2:59914745-59914764 Msa0414410:CDS 30.0%
TATGTATGATATGCAAAGAG+AGG - chr3_2:59914781-59914800 None:intergenic 30.0%
ACTGGAACAAAGTAAAAAGA+TGG + chr3_2:59915108-59915127 Msa0414410:CDS 30.0%
CAAATTCTCAGCAATAAAAG+AGG - chr3_2:59915302-59915321 None:intergenic 30.0%
! CTTTTATTGCTGAGAATTTG+AGG + chr3_2:59915302-59915321 Msa0414410:CDS 30.0%
GATGAAATTAGCACTCTTTG+TGG + chr3_2:59914977-59914996 Msa0414410:CDS 35.0%
! TTTTTACTTTGTTCCAGTCC+AGG - chr3_2:59915106-59915125 None:intergenic 35.0%
GGAACAAAGTAAAAAGATGG+TGG + chr3_2:59915111-59915130 Msa0414410:CDS 35.0%
!! TAAAAAGATGGTGGATCAAG+AGG + chr3_2:59915120-59915139 Msa0414410:CDS 35.0%
! TATTCTTGAGGCAAAGCATT+GGG + chr3_2:59915143-59915162 Msa0414410:CDS 35.0%
!! ATGAGCAGTTGATGAAACAA+AGG + chr3_2:59915173-59915192 Msa0414410:CDS 35.0%
ACATAACATGTGACTCGAAG+AGG + chr3_2:59914716-59914735 Msa0414410:CDS 40.0%
ACTCTTTGTGGAATAGAAGC+TGG + chr3_2:59914989-59915008 Msa0414410:CDS 40.0%
!! TGGATCAAGAGGTATTCTTG+AGG + chr3_2:59915131-59915150 Msa0414410:CDS 40.0%
GTATTCTTGAGGCAAAGCAT+TGG + chr3_2:59915142-59915161 Msa0414410:CDS 40.0%
!! GCAGTTGATGAAACAAAGGA+AGG + chr3_2:59915177-59915196 Msa0414410:CDS 40.0%
ATTGGTCATTTCCTGCTTTC+TGG - chr3_2:59915206-59915225 None:intergenic 40.0%
TATTGCTGAGAATTTGAGGG+AGG + chr3_2:59915306-59915325 Msa0414410:CDS 40.0%
GAGGTCGATCAAAAGATGAA+AGG + chr3_2:59915325-59915344 Msa0414410:CDS 40.0%
TAACATGTGACTCGAAGAGG+AGG + chr3_2:59914719-59914738 Msa0414410:CDS 45.0%
GGAGGATGACATTCAGAAAG+AGG + chr3_2:59914737-59914756 Msa0414410:CDS 45.0%
TGAATCTGATGGCCAAACCT+GGG - chr3_2:59915047-59915066 None:intergenic 45.0%
AGGTTTGGCCATCAGATTCA+GGG + chr3_2:59915047-59915066 Msa0414410:CDS 45.0%
CTTCTAACCCCTGAATCTGA+TGG - chr3_2:59915058-59915077 None:intergenic 45.0%
! GGGTTAGAAGTGTGATATCC+AGG + chr3_2:59915068-59915087 Msa0414410:CDS 45.0%
GAAGTGTGATATCCAGGTTC+AGG + chr3_2:59915074-59915093 Msa0414410:CDS 45.0%
AAGGAAGGAAACCAGAAAGC+AGG + chr3_2:59915192-59915211 Msa0414410:CDS 45.0%
! CCTGGGCTTGAGGATTGTTT+TGG - chr3_2:59915030-59915049 None:intergenic 50.0%
CCAAAACAATCCTCAAGCCC+AGG + chr3_2:59915027-59915046 Msa0414410:CDS 50.0%
ACAATCCTCAAGCCCAGGTT+TGG + chr3_2:59915032-59915051 Msa0414410:CDS 50.0%
CTGAATCTGATGGCCAAACC+TGG - chr3_2:59915048-59915067 None:intergenic 50.0%
CAGGTTTGGCCATCAGATTC+AGG + chr3_2:59915046-59915065 Msa0414410:CDS 50.0%
GGTTTGGCCATCAGATTCAG+GGG + chr3_2:59915048-59915067 Msa0414410:CDS 50.0%
TCCAGGTTCAGGAGTTTGCC+TGG + chr3_2:59915085-59915104 Msa0414410:CDS 55.0%
TCCAGGCAAACTCCTGAACC+TGG - chr3_2:59915089-59915108 None:intergenic 55.0%
GTTCAGGAGTTTGCCTGGAC+TGG + chr3_2:59915090-59915109 Msa0414410:CDS 55.0%
GATGGCCAAACCTGGGCTTG+AGG - chr3_2:59915040-59915059 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59914688 59915366 59914688 ID=Msa0414410;Name=Msa0414410
chr3_2 mRNA 59914688 59915366 59914688 ID=Msa0414410-mRNA-1;Parent=Msa0414410;Name=Msa0414410-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|161
chr3_2 exon 59914688 59914766 59914688 ID=Msa0414410-mRNA-1:exon:12455;Parent=Msa0414410-mRNA-1
chr3_2 exon 59914960 59915366 59914960 ID=Msa0414410-mRNA-1:exon:12456;Parent=Msa0414410-mRNA-1
chr3_2 CDS 59914688 59914766 59914688 ID=Msa0414410-mRNA-1:cds;Parent=Msa0414410-mRNA-1
chr3_2 CDS 59914960 59915366 59914960 ID=Msa0414410-mRNA-1:cds;Parent=Msa0414410-mRNA-1
Gene Sequence

>Msa0414410

ATGGCTAGAGGGAAGGTGAAACTAGCATACATAACATGTGACTCGAAGAGGAGGATGACATTCAGAAAGAGGAAAAATGGAATTATGAAGAAGATTGATGAAATTAGCACTCTTTGTGGAATAGAAGCTGGTGCAATAATTTACGACCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAGGAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGTTGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACATGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGCTAATCGAAGATTGA

Protein sequence

>Msa0414410

MARGKVKLAYITCDSKRRMTFRKRKNGIMKKIDEISTLCGIEAGAIIYDQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQLMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKHDLNDLSSFIAENLREVDQKMKGMLIED*