Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367340 | MCI08872.1 | 77.551 | 98 | 18 | 1 | 1 | 98 | 1 | 94 | 4.72e-47 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367340 | sp|Q93Z79|C14A1_ARATH | 33.333 | 93 | 60 | 1 | 13 | 103 | 15 | 107 | 1.16e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367340 | A0A392PAV4 | 77.551 | 98 | 18 | 1 | 1 | 98 | 1 | 94 | 2.25e-47 | 158 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0108350 | Msa0367340 | 0.816839 | 4.495521e-52 | -8.615850e-47 |
| Msa0163340 | Msa0367340 | 0.800036 | 1.740115e-48 | -8.615850e-47 |
| Msa0190670 | Msa0367340 | 0.806268 | 8.931896e-50 | -8.615850e-47 |
| Msa0367340 | Msa0554660 | 0.802118 | 6.530110e-49 | -8.615850e-47 |
| Msa0367340 | Msa0591490 | 0.803456 | 3.456908e-49 | -8.615850e-47 |
| Msa0367340 | Msa0727480 | 0.816449 | 5.497934e-52 | -8.615850e-47 |
| Msa0367340 | Msa0739720 | 0.824014 | 1.015276e-53 | -8.615850e-47 |
| Msa0367340 | Msa0814160 | 0.805019 | 1.633594e-49 | -8.615850e-47 |
| Msa0367340 | Msa0816140 | 0.805842 | 1.098367e-49 | -8.615850e-47 |
| Msa0367340 | Msa0824850 | 0.800707 | 1.270240e-48 | -8.615850e-47 |
| Msa0367340 | Msa0854840 | 0.804307 | 2.299844e-49 | -8.615850e-47 |
| Msa0367340 | Msa0896920 | 0.819994 | 8.671128e-53 | -8.615850e-47 |
| Msa0367340 | Msa0896950 | 0.804443 | 2.155083e-49 | -8.615850e-47 |
| Msa0367340 | Msa0910830 | 0.804103 | 2.536456e-49 | -8.615850e-47 |
| Msa0367340 | Msa1006170 | 0.828688 | 7.824974e-55 | -8.615850e-47 |
| Msa0367340 | Msa1061670 | 0.815213 | 1.037312e-51 | -8.615850e-47 |
| Msa0367340 | Msa1064940 | 0.801685 | 8.011198e-49 | -8.615850e-47 |
| Msa0367340 | Msa1106680 | 0.807323 | 5.346471e-50 | -8.615850e-47 |
| Msa0367340 | Msa1106960 | 0.801279 | 9.704547e-49 | -8.615850e-47 |
| Msa0367340 | Msa1148210 | 0.812263 | 4.629810e-51 | -8.615850e-47 |
| Msa0367340 | Msa1152180 | 0.822378 | 2.445714e-53 | -8.615850e-47 |
| Msa0367340 | Msa1170340 | 0.810918 | 9.077961e-51 | -8.615850e-47 |
| Msa0367340 | Msa1294020 | 0.809746 | 1.624485e-50 | -8.615850e-47 |
| Msa0367340 | Msa1323120 | 0.822491 | 2.302699e-53 | -8.615850e-47 |
| Msa0367340 | Msa1377520 | 0.800949 | 1.133419e-48 | -8.615850e-47 |
| Msa0367340 | Msa1407670 | 0.802056 | 6.724032e-49 | -8.615850e-47 |
| Msa0367340 | Msa1411550 | 0.812790 | 3.549995e-51 | -8.615850e-47 |
| Msa0367340 | Msa1459610 | 0.829320 | 5.498869e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367340 | MtrunA17_Chr3g0128461 | 34.000 | 100 | 65 | 1 | 5 | 103 | 7 | 106 | 4.76e-14 | 66.6 |
| Msa0367340 | MtrunA17_Chr8g0336361 | 36.264 | 91 | 58 | 0 | 13 | 103 | 14 | 104 | 9.76e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0367340 | AT5G24910.1 | 33.333 | 93 | 60 | 1 | 13 | 103 | 15 | 107 | 1.18e-13 | 65.9 |
| Msa0367340 | AT5G24910.2 | 33.333 | 93 | 60 | 1 | 13 | 103 | 15 | 107 | 1.44e-13 | 65.5 |
| Msa0367340 | AT5G24900.1 | 28.571 | 91 | 63 | 1 | 13 | 103 | 15 | 103 | 1.83e-11 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACATCCATCTTGTTCAT+TGG | 0.324670 | 3_1:+63028829 | Msa0367340:CDS |
| AGCTTAGAACGAAGAACATT+TGG | 0.351655 | 3_1:-63028898 | None:intergenic |
| TATATTGCTTGGAAATCTAA+TGG | 0.375673 | 3_1:+63028954 | Msa0367340:CDS |
| TCTTTCCTAAGTTTGAAGAA+TGG | 0.446291 | 3_1:+63029058 | Msa0367340:CDS |
| TCCCTCCATTCTTCAAACTT+AGG | 0.451351 | 3_1:-63029063 | None:intergenic |
| AGATTTCCAAGCAATATAGT+TGG | 0.461060 | 3_1:-63028949 | None:intergenic |
| GAAGAATGGAGGGAACAATA+TGG | 0.462950 | 3_1:+63029072 | Msa0367340:CDS |
| CAGTGTTGTGAGTAAGAAGA+GGG | 0.483631 | 3_1:-63029026 | None:intergenic |
| CCACCTCCAACTATATTGCT+TGG | 0.501476 | 3_1:+63028943 | Msa0367340:CDS |
| TCCTAAGTTTGAAGAATGGA+GGG | 0.509425 | 3_1:+63029062 | Msa0367340:CDS |
| CTAAGGAAACAAGGTATAAG+TGG | 0.516755 | 3_1:+63028919 | Msa0367340:CDS |
| ATGTTCTTCGTTCTAAGCTA+AGG | 0.519927 | 3_1:+63028902 | Msa0367340:CDS |
| TTCCTAAGTTTGAAGAATGG+AGG | 0.523863 | 3_1:+63029061 | Msa0367340:CDS |
| CCAAGCAATATAGTTGGAGG+TGG | 0.527409 | 3_1:-63028943 | None:intergenic |
| CGTTCTAAGCTAAGGAAACA+AGG | 0.537986 | 3_1:+63028910 | Msa0367340:CDS |
| TTTCCAAGCAATATAGTTGG+AGG | 0.546199 | 3_1:-63028946 | None:intergenic |
| GCAGTGTTGTGAGTAAGAAG+AGG | 0.562261 | 3_1:-63029027 | None:intergenic |
| AAAAGAAGGAGAATTTGAAG+TGG | 0.582573 | 3_1:-63028998 | None:intergenic |
| AGTGTTGTGAGTAAGAAGAG+GGG | 0.608148 | 3_1:-63029025 | None:intergenic |
| AACAAAACCAATGAACAAGA+TGG | 0.609778 | 3_1:-63028836 | None:intergenic |
| GTGTTGTGAGTAAGAAGAGG+GGG | 0.625576 | 3_1:-63029024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATAATTGATAAATACAAT+TGG | - | chr3_1:63029121-63029140 | None:intergenic | 15.0% |
| !! | TATCATATTATCTATGATCA+GGG | + | chr3_1:63029152-63029171 | Msa0367340:intron | 20.0% |
| ! | TGAAAAACTGCAAATCAAAT+TGG | - | chr3_1:63028813-63028832 | None:intergenic | 25.0% |
| ! | TATATTGCTTGGAAATCTAA+TGG | + | chr3_1:63028954-63028973 | Msa0367340:CDS | 25.0% |
| ! | CATAGATAATATGATACAGA+TGG | - | chr3_1:63029148-63029167 | None:intergenic | 25.0% |
| ! | GTATCATATTATCTATGATC+AGG | + | chr3_1:63029151-63029170 | Msa0367340:intron | 25.0% |
| AACAAAACCAATGAACAAGA+TGG | - | chr3_1:63028839-63028858 | None:intergenic | 30.0% | |
| AGATTTCCAAGCAATATAGT+TGG | - | chr3_1:63028952-63028971 | None:intergenic | 30.0% | |
| AAAAGAAGGAGAATTTGAAG+TGG | - | chr3_1:63029001-63029020 | None:intergenic | 30.0% | |
| TCTTTCCTAAGTTTGAAGAA+TGG | + | chr3_1:63029058-63029077 | Msa0367340:CDS | 30.0% | |
| TTCACATCCATCTTGTTCAT+TGG | + | chr3_1:63028829-63028848 | Msa0367340:CDS | 35.0% | |
| AGCTTAGAACGAAGAACATT+TGG | - | chr3_1:63028901-63028920 | None:intergenic | 35.0% | |
| ! | ATGTTCTTCGTTCTAAGCTA+AGG | + | chr3_1:63028902-63028921 | Msa0367340:CDS | 35.0% |
| CTAAGGAAACAAGGTATAAG+TGG | + | chr3_1:63028919-63028938 | Msa0367340:CDS | 35.0% | |
| TTTCCAAGCAATATAGTTGG+AGG | - | chr3_1:63028949-63028968 | None:intergenic | 35.0% | |
| TTCCTAAGTTTGAAGAATGG+AGG | + | chr3_1:63029061-63029080 | Msa0367340:CDS | 35.0% | |
| TCCTAAGTTTGAAGAATGGA+GGG | + | chr3_1:63029062-63029081 | Msa0367340:CDS | 35.0% | |
| ATATTATCTATGATCAGGGC+CGG | + | chr3_1:63029156-63029175 | Msa0367340:intron | 35.0% | |
| ! | CGTTCTAAGCTAAGGAAACA+AGG | + | chr3_1:63028910-63028929 | Msa0367340:CDS | 40.0% |
| AGTGTTGTGAGTAAGAAGAG+GGG | - | chr3_1:63029028-63029047 | None:intergenic | 40.0% | |
| CAGTGTTGTGAGTAAGAAGA+GGG | - | chr3_1:63029029-63029048 | None:intergenic | 40.0% | |
| TCCCTCCATTCTTCAAACTT+AGG | - | chr3_1:63029066-63029085 | None:intergenic | 40.0% | |
| GAAGAATGGAGGGAACAATA+TGG | + | chr3_1:63029072-63029091 | Msa0367340:CDS | 40.0% | |
| CCAAGCAATATAGTTGGAGG+TGG | - | chr3_1:63028946-63028965 | None:intergenic | 45.0% | |
| CCACCTCCAACTATATTGCT+TGG | + | chr3_1:63028943-63028962 | Msa0367340:CDS | 45.0% | |
| AGAAGAGGGGGTTCAAAAGA+AGG | - | chr3_1:63029015-63029034 | None:intergenic | 45.0% | |
| GTGTTGTGAGTAAGAAGAGG+GGG | - | chr3_1:63029027-63029046 | None:intergenic | 45.0% | |
| GCAGTGTTGTGAGTAAGAAG+AGG | - | chr3_1:63029030-63029049 | None:intergenic | 45.0% | |
| GGCACCAAAAATCTTAGGAC+CGG | - | chr3_1:63029178-63029197 | None:intergenic | 45.0% | |
| !!! | TCCTAAGATTTTTGGTGCCC+TGG | + | chr3_1:63029179-63029198 | Msa0367340:CDS | 45.0% |
| ! | TCCAGGGCACCAAAAATCTT+AGG | - | chr3_1:63029183-63029202 | None:intergenic | 45.0% |
| ! | AGGGCCGGTCCTAAGATTTT+TGG | + | chr3_1:63029171-63029190 | Msa0367340:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 63028802 | 63029213 | 63028802 | ID=Msa0367340;Name=Msa0367340 |
| chr3_1 | mRNA | 63028802 | 63029213 | 63028802 | ID=Msa0367340-mRNA-1;Parent=Msa0367340;Name=Msa0367340-mRNA-1;_AED=0.52;_eAED=0.55;_QI=0|0|0|0.5|1|1|2|0|110 |
| chr3_1 | exon | 63028802 | 63029093 | 63028802 | ID=Msa0367340-mRNA-1:exon:12356;Parent=Msa0367340-mRNA-1 |
| chr3_1 | exon | 63029173 | 63029213 | 63029173 | ID=Msa0367340-mRNA-1:exon:12357;Parent=Msa0367340-mRNA-1 |
| chr3_1 | CDS | 63028802 | 63029093 | 63028802 | ID=Msa0367340-mRNA-1:cds;Parent=Msa0367340-mRNA-1 |
| chr3_1 | CDS | 63029173 | 63029213 | 63029173 | ID=Msa0367340-mRNA-1:cds;Parent=Msa0367340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
