Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0371810 | GAU44153.1 | 63.402 | 194 | 53 | 6 | 8 | 183 | 169 | 362 | 3.88e-62 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0371810 | sp|F4K6X0|CHR42_ARATH | 62.963 | 81 | 27 | 2 | 103 | 181 | 38 | 117 | 5.94e-25 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0371810 | A0A2Z6NPY8 | 63.402 | 194 | 53 | 6 | 8 | 183 | 169 | 362 | 1.85e-62 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011720 | Msa0371810 | 0.808707 | 2.712860e-50 | -8.615850e-47 |
| Msa0012440 | Msa0371810 | 0.814365 | 1.599089e-51 | -8.615850e-47 |
| Msa0057240 | Msa0371810 | 0.805026 | 1.628018e-49 | -8.615850e-47 |
| Msa0149560 | Msa0371810 | 0.806464 | 8.121237e-50 | -8.615850e-47 |
| Msa0173990 | Msa0371810 | 0.846654 | 1.896069e-59 | -8.615850e-47 |
| Msa0235890 | Msa0371810 | 0.824461 | 7.969685e-54 | -8.615850e-47 |
| Msa0371810 | Msa0426330 | 0.810679 | 1.022551e-50 | -8.615850e-47 |
| Msa0371810 | Msa0466060 | 0.805345 | 1.396096e-49 | -8.615850e-47 |
| Msa0371810 | Msa0466220 | 0.867000 | 1.888977e-65 | -8.615850e-47 |
| Msa0371810 | Msa0466280 | 0.967629 | 1.530155e-127 | -8.615850e-47 |
| Msa0371810 | Msa0473240 | 0.807591 | 4.689760e-50 | -8.615850e-47 |
| Msa0371810 | Msa0484780 | 0.801635 | 8.203244e-49 | -8.615850e-47 |
| Msa0371810 | Msa0517330 | 0.804458 | 2.139261e-49 | -8.615850e-47 |
| Msa0371810 | Msa0526890 | 0.803661 | 3.133703e-49 | -8.615850e-47 |
| Msa0371810 | Msa0788710 | 0.806224 | 9.127210e-50 | -8.615850e-47 |
| Msa0371810 | Msa0870620 | 0.841285 | 5.212545e-58 | -8.615850e-47 |
| Msa0371810 | Msa0870690 | 0.821397 | 4.126237e-53 | -8.615850e-47 |
| Msa0371810 | Msa1027290 | 0.808152 | 3.563117e-50 | -8.615850e-47 |
| Msa0371810 | Msa1082500 | 0.801687 | 8.004046e-49 | -8.615850e-47 |
| Msa0371810 | Msa1198090 | 0.812764 | 3.597958e-51 | -8.615850e-47 |
| Msa0371810 | Msa1199560 | 0.802464 | 5.542247e-49 | -8.615850e-47 |
| Msa0371810 | Msa1233500 | 0.805395 | 1.363095e-49 | -8.615850e-47 |
| Msa0371810 | Msa1328700 | 0.801384 | 9.237641e-49 | -8.615850e-47 |
| Msa0371810 | Msa1429420 | 0.811645 | 6.311913e-51 | -8.615850e-47 |
| Msa0323080 | Msa0371810 | 0.800827 | 1.200851e-48 | -8.615850e-47 |
| Msa0352000 | Msa0371810 | 0.812750 | 3.622675e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0371810 | MtrunA17_Chr3g0119011 | 65.625 | 128 | 35 | 4 | 64 | 183 | 2 | 128 | 5.55e-46 | 147 |
| Msa0371810 | MtrunA17_Chr3g0118991 | 94.915 | 59 | 3 | 0 | 125 | 183 | 1 | 59 | 8.07e-38 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0371810 | AT5G20935.1 | 62.963 | 81 | 27 | 2 | 103 | 181 | 38 | 117 | 6.03e-26 | 96.7 |
Find 36 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAAATCAACTGGAATATT+TGG | 0.198763 | 3_1:+70053154 | None:intergenic |
| ACGTTTGTACAAGCCAAAAT+TGG | 0.210526 | 3_1:+70053012 | None:intergenic |
| CTGTTGTGGAACATTGATTT+TGG | 0.228912 | 3_1:+70053180 | None:intergenic |
| TGGAGCTTCAATGATAATAA+TGG | 0.263065 | 3_1:+70053072 | None:intergenic |
| GCTATACTCGGCCATATCTA+AGG | 0.274936 | 3_1:+70052676 | None:intergenic |
| AAACGTTCAAAGTTGGATAA+AGG | 0.314200 | 3_1:-70053100 | Msa0371810:CDS |
| TCGGTTTATTGCCCCAATTT+TGG | 0.319384 | 3_1:-70053025 | Msa0371810:CDS |
| ATTGGAACTTATATTATTGA+TGG | 0.349289 | 3_1:-70052713 | Msa0371810:CDS |
| GGTCAAAACTGCAGCTAATT+CGG | 0.362866 | 3_1:-70053044 | Msa0371810:CDS |
| CGTTTGTACAAGCCAAAATT+GGG | 0.376043 | 3_1:+70053013 | None:intergenic |
| TTGGTGCCGACCTTCAAATA+TGG | 0.377926 | 3_1:+70053199 | None:intergenic |
| GGAGCTTCAATGATAATAAT+GGG | 0.378832 | 3_1:+70053073 | None:intergenic |
| ATATTTCAAGCCCTTAGATA+TGG | 0.418211 | 3_1:-70052687 | Msa0371810:CDS |
| AACGTTCAAAGTTGGATAAA+GGG | 0.431347 | 3_1:-70053099 | Msa0371810:CDS |
| CGTGACCTCAGCAAATCAAC+TGG | 0.460489 | 3_1:+70053145 | None:intergenic |
| TACCACCAAGCCATATTTGA+AGG | 0.463739 | 3_1:-70053209 | Msa0371810:CDS |
| TGGGCAGTGCGGCGCAGCAT+TGG | 0.466372 | 3_1:-70052731 | Msa0371810:CDS |
| TATCATTGAAGCTCCAAAGA+TGG | 0.502814 | 3_1:-70053065 | Msa0371810:CDS |
| CTTGGTGGTAGAGCAAGAAA+TGG | 0.514075 | 3_1:+70053222 | None:intergenic |
| CTTAATCAGCTAGCTATACT+CGG | 0.522059 | 3_1:+70052664 | None:intergenic |
| GGAATATTTGGTATCTGTTG+TGG | 0.533879 | 3_1:+70053166 | None:intergenic |
| TCAATCCTATCATATGAATG+AGG | 0.535136 | 3_1:+70053400 | None:intergenic |
| CAATCCTATCATATGAATGA+GGG | 0.550534 | 3_1:+70053401 | None:intergenic |
| GACCTTCAAATATGGCTTGG+TGG | 0.555798 | 3_1:+70053207 | None:intergenic |
| CTATACTCGGCCATATCTAA+GGG | 0.573177 | 3_1:+70052677 | None:intergenic |
| GATAAAGTTATAAACAGTGT+AGG | 0.575222 | 3_1:-70053265 | Msa0371810:CDS |
| GCCGACCTTCAAATATGGCT+TGG | 0.578715 | 3_1:+70053204 | None:intergenic |
| ATATTCCAGTTGATTTGCTG+AGG | 0.581675 | 3_1:-70053150 | Msa0371810:CDS |
| TGAAGAGAAATCGTCACTGA+AGG | 0.602801 | 3_1:-70053299 | Msa0371810:CDS |
| ACCAAGCCATATTTGAAGGT+CGG | 0.616443 | 3_1:-70053205 | Msa0371810:CDS |
| TTGTACAAACGTGGTGATGT+TGG | 0.626789 | 3_1:-70053001 | Msa0371810:CDS |
| ATCCTATCATATGAATGAGG+GGG | 0.635069 | 3_1:+70053403 | None:intergenic |
| AATCCTATCATATGAATGAG+GGG | 0.646986 | 3_1:+70053402 | None:intergenic |
| ACAAACGTGGTGATGTTGGA+AGG | 0.684402 | 3_1:-70052997 | Msa0371810:intron |
| AAGAAGATGTGTGGGCAGTG+CGG | 0.684625 | 3_1:-70052742 | Msa0371810:CDS |
| GTTTGTACAAGCCAAAATTG+GGG | 0.698986 | 3_1:+70053014 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTCTAAAGATTAATTAAT+TGG | + | chr3_1:70053222-70053241 | None:intergenic | 15.0% |
| !! | TTGTCTAAAGATTAATTAAT+TGG | + | chr3_1:70053222-70053241 | None:intergenic | 15.0% |
| !! | ATTGGAACTTATATTATTGA+TGG | - | chr3_1:70053378-70053397 | Msa0371810:CDS | 20.0% |
| !! | ATTGGAACTTATATTATTGA+TGG | - | chr3_1:70053378-70053397 | Msa0371810:CDS | 20.0% |
| ! | GATAAAGTTATAAACAGTGT+AGG | - | chr3_1:70052826-70052845 | Msa0371810:intron | 25.0% |
| ! | TGATGAAAAATTAAGAGATG+AGG | + | chr3_1:70053127-70053146 | None:intergenic | 25.0% |
| ! | GATAAAGTTATAAACAGTGT+AGG | - | chr3_1:70052826-70052845 | Msa0371810:intron | 25.0% |
| ! | TGATGAAAAATTAAGAGATG+AGG | + | chr3_1:70053127-70053146 | None:intergenic | 25.0% |
| AATCCTATCATATGAATGAG+GGG | + | chr3_1:70052692-70052711 | None:intergenic | 30.0% | |
| CAATCCTATCATATGAATGA+GGG | + | chr3_1:70052693-70052712 | None:intergenic | 30.0% | |
| TCAATCCTATCATATGAATG+AGG | + | chr3_1:70052694-70052713 | None:intergenic | 30.0% | |
| AGCAAATCAACTGGAATATT+TGG | + | chr3_1:70052940-70052959 | None:intergenic | 30.0% | |
| AAACGTTCAAAGTTGGATAA+AGG | - | chr3_1:70052991-70053010 | Msa0371810:intron | 30.0% | |
| AACGTTCAAAGTTGGATAAA+GGG | - | chr3_1:70052992-70053011 | Msa0371810:intron | 30.0% | |
| GGAGCTTCAATGATAATAAT+GGG | + | chr3_1:70053021-70053040 | None:intergenic | 30.0% | |
| TGGAGCTTCAATGATAATAA+TGG | + | chr3_1:70053022-70053041 | None:intergenic | 30.0% | |
| AAAAAAGCAAGAAGATGTGT+GGG | - | chr3_1:70053341-70053360 | Msa0371810:CDS | 30.0% | |
| ATATTTCAAGCCCTTAGATA+TGG | - | chr3_1:70053404-70053423 | Msa0371810:CDS | 30.0% | |
| AATCCTATCATATGAATGAG+GGG | + | chr3_1:70052692-70052711 | None:intergenic | 30.0% | |
| CAATCCTATCATATGAATGA+GGG | + | chr3_1:70052693-70052712 | None:intergenic | 30.0% | |
| TCAATCCTATCATATGAATG+AGG | + | chr3_1:70052694-70052713 | None:intergenic | 30.0% | |
| AGCAAATCAACTGGAATATT+TGG | + | chr3_1:70052940-70052959 | None:intergenic | 30.0% | |
| AAACGTTCAAAGTTGGATAA+AGG | - | chr3_1:70052991-70053010 | Msa0371810:intron | 30.0% | |
| AACGTTCAAAGTTGGATAAA+GGG | - | chr3_1:70052992-70053011 | Msa0371810:intron | 30.0% | |
| GGAGCTTCAATGATAATAAT+GGG | + | chr3_1:70053021-70053040 | None:intergenic | 30.0% | |
| TGGAGCTTCAATGATAATAA+TGG | + | chr3_1:70053022-70053041 | None:intergenic | 30.0% | |
| AAAAAAGCAAGAAGATGTGT+GGG | - | chr3_1:70053341-70053360 | Msa0371810:CDS | 30.0% | |
| ATATTTCAAGCCCTTAGATA+TGG | - | chr3_1:70053404-70053423 | Msa0371810:CDS | 30.0% | |
| ATCCTATCATATGAATGAGG+GGG | + | chr3_1:70052691-70052710 | None:intergenic | 35.0% | |
| !!! | CTGTTGTGGAACATTGATTT+TGG | + | chr3_1:70052914-70052933 | None:intergenic | 35.0% |
| GGAATATTTGGTATCTGTTG+TGG | + | chr3_1:70052928-70052947 | None:intergenic | 35.0% | |
| !! | ATATTCCAGTTGATTTGCTG+AGG | - | chr3_1:70052941-70052960 | Msa0371810:intron | 35.0% |
| ! | CGTTTTTCTTGCAACTCTTT+TGG | + | chr3_1:70052976-70052995 | None:intergenic | 35.0% |
| GCAAGAAAAACGTTCAAAGT+TGG | - | chr3_1:70052984-70053003 | Msa0371810:intron | 35.0% | |
| TATCATTGAAGCTCCAAAGA+TGG | - | chr3_1:70053026-70053045 | Msa0371810:CDS | 35.0% | |
| GTTTGTACAAGCCAAAATTG+GGG | + | chr3_1:70053080-70053099 | None:intergenic | 35.0% | |
| CGTTTGTACAAGCCAAAATT+GGG | + | chr3_1:70053081-70053100 | None:intergenic | 35.0% | |
| ACGTTTGTACAAGCCAAAAT+TGG | + | chr3_1:70053082-70053101 | None:intergenic | 35.0% | |
| !!! | AATTTTGGCTTGTACAAACG+TGG | - | chr3_1:70053081-70053100 | Msa0371810:CDS | 35.0% |
| GCAGTAGCAACTAATTGATT+TGG | - | chr3_1:70053178-70053197 | Msa0371810:CDS | 35.0% | |
| CAAAAAAGCAAGAAGATGTG+TGG | - | chr3_1:70053340-70053359 | Msa0371810:CDS | 35.0% | |
| ATCCTATCATATGAATGAGG+GGG | + | chr3_1:70052691-70052710 | None:intergenic | 35.0% | |
| !!! | CTGTTGTGGAACATTGATTT+TGG | + | chr3_1:70052914-70052933 | None:intergenic | 35.0% |
| GGAATATTTGGTATCTGTTG+TGG | + | chr3_1:70052928-70052947 | None:intergenic | 35.0% | |
| !! | ATATTCCAGTTGATTTGCTG+AGG | - | chr3_1:70052941-70052960 | Msa0371810:intron | 35.0% |
| ! | CGTTTTTCTTGCAACTCTTT+TGG | + | chr3_1:70052976-70052995 | None:intergenic | 35.0% |
| GCAAGAAAAACGTTCAAAGT+TGG | - | chr3_1:70052984-70053003 | Msa0371810:intron | 35.0% | |
| TATCATTGAAGCTCCAAAGA+TGG | - | chr3_1:70053026-70053045 | Msa0371810:CDS | 35.0% | |
| GTTTGTACAAGCCAAAATTG+GGG | + | chr3_1:70053080-70053099 | None:intergenic | 35.0% | |
| CGTTTGTACAAGCCAAAATT+GGG | + | chr3_1:70053081-70053100 | None:intergenic | 35.0% | |
| ACGTTTGTACAAGCCAAAAT+TGG | + | chr3_1:70053082-70053101 | None:intergenic | 35.0% | |
| !!! | AATTTTGGCTTGTACAAACG+TGG | - | chr3_1:70053081-70053100 | Msa0371810:CDS | 35.0% |
| GCAGTAGCAACTAATTGATT+TGG | - | chr3_1:70053178-70053197 | Msa0371810:CDS | 35.0% | |
| CAAAAAAGCAAGAAGATGTG+TGG | - | chr3_1:70053340-70053359 | Msa0371810:CDS | 35.0% | |
| ATATGAATGAGGGGGAAAGA+AGG | + | chr3_1:70052683-70052702 | None:intergenic | 40.0% | |
| TTCCCCCTCATTCATATGAT+AGG | - | chr3_1:70052686-70052705 | Msa0371810:CDS | 40.0% | |
| TGAAGAGAAATCGTCACTGA+AGG | - | chr3_1:70052792-70052811 | Msa0371810:intron | 40.0% | |
| TACCACCAAGCCATATTTGA+AGG | - | chr3_1:70052882-70052901 | Msa0371810:intron | 40.0% | |
| ACCAAGCCATATTTGAAGGT+CGG | - | chr3_1:70052886-70052905 | Msa0371810:intron | 40.0% | |
| GGTCAAAACTGCAGCTAATT+CGG | - | chr3_1:70053047-70053066 | Msa0371810:CDS | 40.0% | |
| ! | TCGGTTTATTGCCCCAATTT+TGG | - | chr3_1:70053066-70053085 | Msa0371810:CDS | 40.0% |
| ! | TTGTACAAACGTGGTGATGT+TGG | - | chr3_1:70053090-70053109 | Msa0371810:CDS | 40.0% |
| CTATACTCGGCCATATCTAA+GGG | + | chr3_1:70053417-70053436 | None:intergenic | 40.0% | |
| ATATGAATGAGGGGGAAAGA+AGG | + | chr3_1:70052683-70052702 | None:intergenic | 40.0% | |
| TTCCCCCTCATTCATATGAT+AGG | - | chr3_1:70052686-70052705 | Msa0371810:CDS | 40.0% | |
| TGAAGAGAAATCGTCACTGA+AGG | - | chr3_1:70052792-70052811 | Msa0371810:intron | 40.0% | |
| TACCACCAAGCCATATTTGA+AGG | - | chr3_1:70052882-70052901 | Msa0371810:intron | 40.0% | |
| ACCAAGCCATATTTGAAGGT+CGG | - | chr3_1:70052886-70052905 | Msa0371810:intron | 40.0% | |
| GGTCAAAACTGCAGCTAATT+CGG | - | chr3_1:70053047-70053066 | Msa0371810:CDS | 40.0% | |
| ! | TCGGTTTATTGCCCCAATTT+TGG | - | chr3_1:70053066-70053085 | Msa0371810:CDS | 40.0% |
| ! | TTGTACAAACGTGGTGATGT+TGG | - | chr3_1:70053090-70053109 | Msa0371810:CDS | 40.0% |
| CTATACTCGGCCATATCTAA+GGG | + | chr3_1:70053417-70053436 | None:intergenic | 40.0% | |
| ! | CTTGGTGGTAGAGCAAGAAA+TGG | + | chr3_1:70052872-70052891 | None:intergenic | 45.0% |
| ! | GACCTTCAAATATGGCTTGG+TGG | + | chr3_1:70052887-70052906 | None:intergenic | 45.0% |
| !! | TTGGTGCCGACCTTCAAATA+TGG | + | chr3_1:70052895-70052914 | None:intergenic | 45.0% |
| !! | GCTGCAGTTTTGACCATCTT+TGG | + | chr3_1:70053042-70053061 | None:intergenic | 45.0% |
| ! | ACAAACGTGGTGATGTTGGA+AGG | - | chr3_1:70053094-70053113 | Msa0371810:CDS | 45.0% |
| GCTATACTCGGCCATATCTA+AGG | + | chr3_1:70053418-70053437 | None:intergenic | 45.0% | |
| ! | CTTGGTGGTAGAGCAAGAAA+TGG | + | chr3_1:70052872-70052891 | None:intergenic | 45.0% |
| ! | GACCTTCAAATATGGCTTGG+TGG | + | chr3_1:70052887-70052906 | None:intergenic | 45.0% |
| !! | TTGGTGCCGACCTTCAAATA+TGG | + | chr3_1:70052895-70052914 | None:intergenic | 45.0% |
| !! | GCTGCAGTTTTGACCATCTT+TGG | + | chr3_1:70053042-70053061 | None:intergenic | 45.0% |
| ! | ACAAACGTGGTGATGTTGGA+AGG | - | chr3_1:70053094-70053113 | Msa0371810:CDS | 45.0% |
| GCTATACTCGGCCATATCTA+AGG | + | chr3_1:70053418-70053437 | None:intergenic | 45.0% | |
| GCCGACCTTCAAATATGGCT+TGG | + | chr3_1:70052890-70052909 | None:intergenic | 50.0% | |
| CGTGACCTCAGCAAATCAAC+TGG | + | chr3_1:70052949-70052968 | None:intergenic | 50.0% | |
| AAGAAGATGTGTGGGCAGTG+CGG | - | chr3_1:70053349-70053368 | Msa0371810:CDS | 50.0% | |
| GCCGACCTTCAAATATGGCT+TGG | + | chr3_1:70052890-70052909 | None:intergenic | 50.0% | |
| CGTGACCTCAGCAAATCAAC+TGG | + | chr3_1:70052949-70052968 | None:intergenic | 50.0% | |
| AAGAAGATGTGTGGGCAGTG+CGG | - | chr3_1:70053349-70053368 | Msa0371810:CDS | 50.0% | |
| TGGGCAGTGCGGCGCAGCAT+TGG | - | chr3_1:70053360-70053379 | Msa0371810:CDS | 70.0% | |
| TGGGCAGTGCGGCGCAGCAT+TGG | - | chr3_1:70053360-70053379 | Msa0371810:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 70052673 | 70053440 | 70052673 | ID=Msa0371810;Name=Msa0371810 |
| chr3_1 | mRNA | 70052673 | 70053440 | 70052673 | ID=Msa0371810-mRNA-1;Parent=Msa0371810;Name=Msa0371810-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|1|0|1|1|1|2|0|183 |
| chr3_1 | exon | 70052998 | 70053440 | 70052998 | ID=Msa0371810-mRNA-1:exon:15080;Parent=Msa0371810-mRNA-1 |
| chr3_1 | exon | 70052673 | 70052781 | 70052673 | ID=Msa0371810-mRNA-1:exon:15079;Parent=Msa0371810-mRNA-1 |
| chr3_1 | CDS | 70052998 | 70053440 | 70052998 | ID=Msa0371810-mRNA-1:cds;Parent=Msa0371810-mRNA-1 |
| chr3_1 | CDS | 70052673 | 70052781 | 70052673 | ID=Msa0371810-mRNA-1:cds;Parent=Msa0371810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
