AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0870690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0870690 MtrunA17_Chr6g0452181 87.619 105 5 1 1 105 1 97 2.09e-57 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCATGACTTATAAGATTT+TGG 0.148800 6_1:-5282444 None:intergenic
AATTAAAATTCGATTTATTT+CGG 0.300225 6_1:+5283381 Msa0870690:three_prime_UTR
TTCTTTCTTGTCATAGATAT+TGG 0.312976 6_1:+5282362 Msa0870690:five_prime_UTR
CAAGAAGAAGTGACAAAATA+AGG 0.315451 6_1:-5282239 None:intergenic
GGGAAGCTTAGCTGTTGATT+TGG 0.366330 6_1:+5282972 Msa0870690:CDS
TTATAAGATGAAAGATGATT+TGG 0.375265 6_1:-5282294 None:intergenic
CGATACTACCTAAATAGTTA+AGG 0.390032 6_1:-5283346 None:intergenic
GTTGGTACTTGGAATGTTGC+TGG 0.407117 6_1:+5282937 Msa0870690:CDS
TTAAGTTTGTATGAAATTGA+AGG 0.414336 6_1:+5282542 Msa0870690:CDS
GAAGGATATGATATATATCA+AGG 0.425478 6_1:-5282422 None:intergenic
ATCAAATTCCAGTGACTGAA+TGG 0.428675 6_1:-5282741 None:intergenic
AAATCTTTCCATTCAGTCAC+TGG 0.441692 6_1:+5282733 Msa0870690:CDS
CTATTTAGGTAGTATCGCTT+TGG 0.444667 6_1:+5283352 Msa0870690:three_prime_UTR
ATTTCATACAAACTTAATGA+TGG 0.452320 6_1:-5282536 None:intergenic
CAAAATAAGGTAGCTTAAGA+TGG 0.462011 6_1:-5282226 None:intergenic
GATACTACCTAAATAGTTAA+GGG 0.473423 6_1:-5283345 None:intergenic
GATATAAGAGGCTATCCAAT+TGG 0.478067 6_1:+5282486 Msa0870690:CDS
ACTGCAATTGAGACCGCATC+AGG 0.481834 6_1:-5283269 None:intergenic
TCTGCAGATATATATGTTCT+CGG 0.485834 6_1:+5283018 Msa0870690:CDS
TTCCAAAATCTTATAAGTCA+TGG 0.487370 6_1:+5282442 Msa0870690:exon
AGATGAGAGTGATGAATATG+AGG 0.493133 6_1:+5282709 Msa0870690:CDS
CAGTTGCGATCAAGTTGTAA+AGG 0.496139 6_1:+5283288 Msa0870690:three_prime_UTR
TTGGTTCACCACCAAACAAA+AGG 0.498272 6_1:+5282505 Msa0870690:CDS
AAGGTAGCTTAAGATGGAGT+TGG 0.506822 6_1:-5282220 None:intergenic
CTGCAGATATATATGTTCTC+GGG 0.531854 6_1:+5283019 Msa0870690:CDS
TTGTTTGGTGGTGAACCAAT+TGG 0.532362 6_1:-5282501 None:intergenic
GCATTTCAACAAATAAGCTA+AGG 0.576448 6_1:+5282768 Msa0870690:CDS
TAGTGTGTTTGTGACTTGCA+TGG 0.581411 6_1:+5282388 Msa0870690:five_prime_UTR
TAGCTGTTGATTTGGATGAG+TGG 0.611111 6_1:+5282980 Msa0870690:CDS
GATGAGAGTGATGAATATGA+GGG 0.613097 6_1:+5282710 Msa0870690:CDS
GTGAACAAATGCTCCTGATG+CGG 0.614909 6_1:+5283256 Msa0870690:three_prime_UTR
TGTTGCTGGAAGATCTCCTG+TGG 0.622172 6_1:+5282951 Msa0870690:CDS
TCAACAGCTAAGCTTCCCAC+AGG 0.622850 6_1:-5282967 None:intergenic
AAGATGAAAGATGATTTGGT+AGG 0.631408 6_1:-5282290 None:intergenic
GTTGCTGGAAGATCTCCTGT+GGG 0.696089 6_1:+5282952 Msa0870690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACAATTTTTTTACATATAA+TGG - chr6_1:5282322-5282341 None:intergenic 10.0%
!! TAGAAAAAAAATATGTATAT+AGG + chr6_1:5282629-5282648 Msa0870690:intron 10.0%
!!! TTAGAAATTTTATAAAATAC+AGG + chr6_1:5283182-5283201 Msa0870690:intron 10.0%
!!! ATAAATCGAATTTTAATTAA+AGG - chr6_1:5283379-5283398 None:intergenic 10.0%
!! AATTAAAATTCGATTTATTT+CGG + chr6_1:5283381-5283400 Msa0870690:three_prime_UTR 10.0%
!!! TACAATTTTTTTACATATAA+TGG - chr6_1:5282322-5282341 None:intergenic 10.0%
!! TAGAAAAAAAATATGTATAT+AGG + chr6_1:5282629-5282648 Msa0870690:intron 10.0%
!!! TTAGAAATTTTATAAAATAC+AGG + chr6_1:5283182-5283201 Msa0870690:intron 10.0%
!!! ATAAATCGAATTTTAATTAA+AGG - chr6_1:5283379-5283398 None:intergenic 10.0%
!! AATTAAAATTCGATTTATTT+CGG + chr6_1:5283381-5283400 Msa0870690:three_prime_UTR 10.0%
!!! AGATTTATTTCTTAATCTAA+CGG - chr6_1:5283111-5283130 None:intergenic 15.0%
!!! TTTTATAAAATACAGGTTAA+TGG + chr6_1:5283189-5283208 Msa0870690:intron 15.0%
!!! AGATTTATTTCTTAATCTAA+CGG - chr6_1:5283111-5283130 None:intergenic 15.0%
!!! TTTTATAAAATACAGGTTAA+TGG + chr6_1:5283189-5283208 Msa0870690:intron 15.0%
!! TTATAAGATGAAAGATGATT+TGG - chr6_1:5282297-5282316 None:intergenic 20.0%
!! ATTTCATACAAACTTAATGA+TGG - chr6_1:5282539-5282558 None:intergenic 20.0%
!! TTAAGTTTGTATGAAATTGA+AGG + chr6_1:5282542-5282561 Msa0870690:CDS 20.0%
!!! ATATTTTTCAGGATTTTTGT+TGG + chr6_1:5282919-5282938 Msa0870690:intron 20.0%
!! TTATAAGATGAAAGATGATT+TGG - chr6_1:5282297-5282316 None:intergenic 20.0%
!! ATTTCATACAAACTTAATGA+TGG - chr6_1:5282539-5282558 None:intergenic 20.0%
!! TTAAGTTTGTATGAAATTGA+AGG + chr6_1:5282542-5282561 Msa0870690:CDS 20.0%
!!! ATATTTTTCAGGATTTTTGT+TGG + chr6_1:5282919-5282938 Msa0870690:intron 20.0%
! TTCTTTCTTGTCATAGATAT+TGG + chr6_1:5282362-5282381 Msa0870690:five_prime_UTR 25.0%
! GAAGGATATGATATATATCA+AGG - chr6_1:5282425-5282444 None:intergenic 25.0%
!! ATGACTTATAAGATTTTGGA+AGG - chr6_1:5282443-5282462 None:intergenic 25.0%
! TTCCAAAATCTTATAAGTCA+TGG + chr6_1:5282442-5282461 Msa0870690:exon 25.0%
! ACCAAAAATCAAGATATAAG+AGG + chr6_1:5282474-5282493 Msa0870690:CDS 25.0%
!! TTTAGGCTAAATTGCATTTT+TGG + chr6_1:5283321-5283340 Msa0870690:three_prime_UTR 25.0%
! GATACTACCTAAATAGTTAA+GGG - chr6_1:5283348-5283367 None:intergenic 25.0%
! TTCTTTCTTGTCATAGATAT+TGG + chr6_1:5282362-5282381 Msa0870690:five_prime_UTR 25.0%
! GAAGGATATGATATATATCA+AGG - chr6_1:5282425-5282444 None:intergenic 25.0%
!! ATGACTTATAAGATTTTGGA+AGG - chr6_1:5282443-5282462 None:intergenic 25.0%
! TTCCAAAATCTTATAAGTCA+TGG + chr6_1:5282442-5282461 Msa0870690:exon 25.0%
! ACCAAAAATCAAGATATAAG+AGG + chr6_1:5282474-5282493 Msa0870690:CDS 25.0%
!! TTTAGGCTAAATTGCATTTT+TGG + chr6_1:5283321-5283340 Msa0870690:three_prime_UTR 25.0%
! GATACTACCTAAATAGTTAA+GGG - chr6_1:5283348-5283367 None:intergenic 25.0%
CAAAATAAGGTAGCTTAAGA+TGG - chr6_1:5282229-5282248 None:intergenic 30.0%
CAAGAAGAAGTGACAAAATA+AGG - chr6_1:5282242-5282261 None:intergenic 30.0%
!! AAGATGAAAGATGATTTGGT+AGG - chr6_1:5282293-5282312 None:intergenic 30.0%
! CTCCATGACTTATAAGATTT+TGG - chr6_1:5282447-5282466 None:intergenic 30.0%
! GCCTCTTATATCTTGATTTT+TGG - chr6_1:5282478-5282497 None:intergenic 30.0%
!! CTTATTTTCCTTTTGTTTGG+TGG - chr6_1:5282516-5282535 None:intergenic 30.0%
!! TGGCTTATTTTCCTTTTGTT+TGG - chr6_1:5282519-5282538 None:intergenic 30.0%
GCATTTCAACAAATAAGCTA+AGG + chr6_1:5282768-5282787 Msa0870690:CDS 30.0%
TTGTGTGATATGTGTTTATG+TGG + chr6_1:5282890-5282909 Msa0870690:intron 30.0%
TCTGCAGATATATATGTTCT+CGG + chr6_1:5283018-5283037 Msa0870690:CDS 30.0%
! GTAAAGGCTTCAAAGTTTTT+AGG + chr6_1:5283304-5283323 Msa0870690:three_prime_UTR 30.0%
! TTTTGGTCCCTTAACTATTT+AGG + chr6_1:5283338-5283357 Msa0870690:three_prime_UTR 30.0%
CGATACTACCTAAATAGTTA+AGG - chr6_1:5283349-5283368 None:intergenic 30.0%
CAAAATAAGGTAGCTTAAGA+TGG - chr6_1:5282229-5282248 None:intergenic 30.0%
CAAGAAGAAGTGACAAAATA+AGG - chr6_1:5282242-5282261 None:intergenic 30.0%
!! AAGATGAAAGATGATTTGGT+AGG - chr6_1:5282293-5282312 None:intergenic 30.0%
! CTCCATGACTTATAAGATTT+TGG - chr6_1:5282447-5282466 None:intergenic 30.0%
! GCCTCTTATATCTTGATTTT+TGG - chr6_1:5282478-5282497 None:intergenic 30.0%
!! CTTATTTTCCTTTTGTTTGG+TGG - chr6_1:5282516-5282535 None:intergenic 30.0%
!! TGGCTTATTTTCCTTTTGTT+TGG - chr6_1:5282519-5282538 None:intergenic 30.0%
GCATTTCAACAAATAAGCTA+AGG + chr6_1:5282768-5282787 Msa0870690:CDS 30.0%
TTGTGTGATATGTGTTTATG+TGG + chr6_1:5282890-5282909 Msa0870690:intron 30.0%
TCTGCAGATATATATGTTCT+CGG + chr6_1:5283018-5283037 Msa0870690:CDS 30.0%
! GTAAAGGCTTCAAAGTTTTT+AGG + chr6_1:5283304-5283323 Msa0870690:three_prime_UTR 30.0%
! TTTTGGTCCCTTAACTATTT+AGG + chr6_1:5283338-5283357 Msa0870690:three_prime_UTR 30.0%
CGATACTACCTAAATAGTTA+AGG - chr6_1:5283349-5283368 None:intergenic 30.0%
GATATAAGAGGCTATCCAAT+TGG + chr6_1:5282486-5282505 Msa0870690:CDS 35.0%
AGATGAGAGTGATGAATATG+AGG + chr6_1:5282709-5282728 Msa0870690:CDS 35.0%
GATGAGAGTGATGAATATGA+GGG + chr6_1:5282710-5282729 Msa0870690:CDS 35.0%
AAATCTTTCCATTCAGTCAC+TGG + chr6_1:5282733-5282752 Msa0870690:CDS 35.0%
ATCAAATTCCAGTGACTGAA+TGG - chr6_1:5282744-5282763 None:intergenic 35.0%
! TGTGGCTAAGCATATTTTTC+AGG + chr6_1:5282908-5282927 Msa0870690:intron 35.0%
! TCAGGATTTTTGTTGGTACT+TGG + chr6_1:5282926-5282945 Msa0870690:intron 35.0%
CTGCAGATATATATGTTCTC+GGG + chr6_1:5283019-5283038 Msa0870690:CDS 35.0%
TTAATCTAACGGCCTACAAT+CGG - chr6_1:5283100-5283119 None:intergenic 35.0%
!!! GGACTCGTAGTTTTAAATTG+TGG + chr6_1:5283210-5283229 Msa0870690:exon 35.0%
CTATTTAGGTAGTATCGCTT+TGG + chr6_1:5283352-5283371 Msa0870690:three_prime_UTR 35.0%
GATATAAGAGGCTATCCAAT+TGG + chr6_1:5282486-5282505 Msa0870690:CDS 35.0%
AGATGAGAGTGATGAATATG+AGG + chr6_1:5282709-5282728 Msa0870690:CDS 35.0%
GATGAGAGTGATGAATATGA+GGG + chr6_1:5282710-5282729 Msa0870690:CDS 35.0%
AAATCTTTCCATTCAGTCAC+TGG + chr6_1:5282733-5282752 Msa0870690:CDS 35.0%
ATCAAATTCCAGTGACTGAA+TGG - chr6_1:5282744-5282763 None:intergenic 35.0%
! TGTGGCTAAGCATATTTTTC+AGG + chr6_1:5282908-5282927 Msa0870690:intron 35.0%
! TCAGGATTTTTGTTGGTACT+TGG + chr6_1:5282926-5282945 Msa0870690:intron 35.0%
CTGCAGATATATATGTTCTC+GGG + chr6_1:5283019-5283038 Msa0870690:CDS 35.0%
TTAATCTAACGGCCTACAAT+CGG - chr6_1:5283100-5283119 None:intergenic 35.0%
!!! GGACTCGTAGTTTTAAATTG+TGG + chr6_1:5283210-5283229 Msa0870690:exon 35.0%
CTATTTAGGTAGTATCGCTT+TGG + chr6_1:5283352-5283371 Msa0870690:three_prime_UTR 35.0%
AAGGTAGCTTAAGATGGAGT+TGG - chr6_1:5282223-5282242 None:intergenic 40.0%
! TAGTGTGTTTGTGACTTGCA+TGG + chr6_1:5282388-5282407 Msa0870690:five_prime_UTR 40.0%
!! TTGTTTGGTGGTGAACCAAT+TGG - chr6_1:5282504-5282523 None:intergenic 40.0%
TTGGTTCACCACCAAACAAA+AGG + chr6_1:5282505-5282524 Msa0870690:CDS 40.0%
!! TAGCTGTTGATTTGGATGAG+TGG + chr6_1:5282980-5282999 Msa0870690:CDS 40.0%
ATACTTAGATTCTCCGCAGT+CGG + chr6_1:5283051-5283070 Msa0870690:intron 40.0%
! CAGTTGCGATCAAGTTGTAA+AGG + chr6_1:5283288-5283307 Msa0870690:three_prime_UTR 40.0%
!! TTCTTTGAGCGTTGAACACA+AGG - chr6_1:5282200-5282219 None:intergenic 40.0%
AAGGTAGCTTAAGATGGAGT+TGG - chr6_1:5282223-5282242 None:intergenic 40.0%
! TAGTGTGTTTGTGACTTGCA+TGG + chr6_1:5282388-5282407 Msa0870690:five_prime_UTR 40.0%
!! TTGTTTGGTGGTGAACCAAT+TGG - chr6_1:5282504-5282523 None:intergenic 40.0%
TTGGTTCACCACCAAACAAA+AGG + chr6_1:5282505-5282524 Msa0870690:CDS 40.0%
!! TAGCTGTTGATTTGGATGAG+TGG + chr6_1:5282980-5282999 Msa0870690:CDS 40.0%
ATACTTAGATTCTCCGCAGT+CGG + chr6_1:5283051-5283070 Msa0870690:intron 40.0%
! CAGTTGCGATCAAGTTGTAA+AGG + chr6_1:5283288-5283307 Msa0870690:three_prime_UTR 40.0%
GTTGGTACTTGGAATGTTGC+TGG + chr6_1:5282937-5282956 Msa0870690:CDS 45.0%
! GGGAAGCTTAGCTGTTGATT+TGG + chr6_1:5282972-5282991 Msa0870690:CDS 45.0%
GTGAACAAATGCTCCTGATG+CGG + chr6_1:5283256-5283275 Msa0870690:three_prime_UTR 45.0%
GTTGGTACTTGGAATGTTGC+TGG + chr6_1:5282937-5282956 Msa0870690:CDS 45.0%
! GGGAAGCTTAGCTGTTGATT+TGG + chr6_1:5282972-5282991 Msa0870690:CDS 45.0%
GTGAACAAATGCTCCTGATG+CGG + chr6_1:5283256-5283275 Msa0870690:three_prime_UTR 45.0%
TGTTGCTGGAAGATCTCCTG+TGG + chr6_1:5282951-5282970 Msa0870690:CDS 50.0%
GTTGCTGGAAGATCTCCTGT+GGG + chr6_1:5282952-5282971 Msa0870690:CDS 50.0%
TCAACAGCTAAGCTTCCCAC+AGG - chr6_1:5282970-5282989 None:intergenic 50.0%
AGCCGACTACAACCGATTGT+AGG + chr6_1:5283085-5283104 Msa0870690:intron 50.0%
GGCCTACAATCGGTTGTAGT+CGG - chr6_1:5283090-5283109 None:intergenic 50.0%
ACTGCAATTGAGACCGCATC+AGG - chr6_1:5283272-5283291 None:intergenic 50.0%
TGTTGCTGGAAGATCTCCTG+TGG + chr6_1:5282951-5282970 Msa0870690:CDS 50.0%
GTTGCTGGAAGATCTCCTGT+GGG + chr6_1:5282952-5282971 Msa0870690:CDS 50.0%
TCAACAGCTAAGCTTCCCAC+AGG - chr6_1:5282970-5282989 None:intergenic 50.0%
AGCCGACTACAACCGATTGT+AGG + chr6_1:5283085-5283104 Msa0870690:intron 50.0%
GGCCTACAATCGGTTGTAGT+CGG - chr6_1:5283090-5283109 None:intergenic 50.0%
ACTGCAATTGAGACCGCATC+AGG - chr6_1:5283272-5283291 None:intergenic 50.0%
CTGCTTCATGCAACCGACTG+CGG - chr6_1:5283067-5283086 None:intergenic 55.0%
CTGCTTCATGCAACCGACTG+CGG - chr6_1:5283067-5283086 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 5282198 5283405 5282198 ID=Msa0870690;Name=Msa0870690
chr6_1 mRNA 5282198 5283405 5282198 ID=Msa0870690-mRNA-1;Parent=Msa0870690;Name=Msa0870690-mRNA-1;_AED=0.05;_eAED=0.05;_QI=263|1|1|1|1|1|4|186|106
chr6_1 exon 5282198 5282563 5282198 ID=Msa0870690-mRNA-1:exon:1715;Parent=Msa0870690-mRNA-1
chr6_1 exon 5282699 5282789 5282699 ID=Msa0870690-mRNA-1:exon:1716;Parent=Msa0870690-mRNA-1
chr6_1 exon 5282930 5283040 5282930 ID=Msa0870690-mRNA-1:exon:1717;Parent=Msa0870690-mRNA-1
chr6_1 exon 5283204 5283405 5283204 ID=Msa0870690-mRNA-1:exon:1718;Parent=Msa0870690-mRNA-1
chr6_1 five_prime_UTR 5282198 5282460 5282198 ID=Msa0870690-mRNA-1:five_prime_utr;Parent=Msa0870690-mRNA-1
chr6_1 CDS 5282461 5282563 5282461 ID=Msa0870690-mRNA-1:cds;Parent=Msa0870690-mRNA-1
chr6_1 CDS 5282699 5282789 5282699 ID=Msa0870690-mRNA-1:cds;Parent=Msa0870690-mRNA-1
chr6_1 CDS 5282930 5283040 5282930 ID=Msa0870690-mRNA-1:cds;Parent=Msa0870690-mRNA-1
chr6_1 CDS 5283204 5283219 5283204 ID=Msa0870690-mRNA-1:cds;Parent=Msa0870690-mRNA-1
chr6_1 three_prime_UTR 5283220 5283405 5283220 ID=Msa0870690-mRNA-1:three_prime_utr;Parent=Msa0870690-mRNA-1
Gene Sequence

>Msa0870690

ATGGAGTTGAAGAACCAAAAATCAAGATATAAGAGGCTATCCAATTGGTTCACCACCAAACAAAAGGAAAATAAGCCATCATTAAGTTTGTATGAAATTGAAGATATAGTTGAAGATGAGAGTGATGAATATGAGGGAAATCTTTCCATTCAGTCACTGGAATTTGATTCTTGCATTTCAACAAATAAGCTAAGGATTTTTGTTGGTACTTGGAATGTTGCTGGAAGATCTCCTGTGGGAAGCTTAGCTGTTGATTTGGATGAGTGGTTAAATCTCAAGAATTCTGCAGATATATATGTTCTCGGGTTAATGGACTCGTAG

Protein sequence

>Msa0870690

MELKNQKSRYKRLSNWFTTKQKENKPSLSLYEIEDIVEDESDEYEGNLSIQSLEFDSCISTNKLRIFVGTWNVAGRSPVGSLAVDLDEWLNLKNSADIYVLGLMDS*