AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0466280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466280 MtrunA17_Chr3g0119011 65.625 128 35 4 64 183 2 128 5.55e-46 147
Msa0466280 MtrunA17_Chr3g0118991 94.915 59 3 0 125 183 1 59 8.07e-38 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466280 AT5G20935.1 62.963 81 27 2 103 181 38 117 6.03e-26 96.7

Find 35 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATCAACTGGAATATT+TGG 0.198763 3_3:+66867965 None:intergenic
ACGTTTGTACAAGCCAAAAT+TGG 0.210526 3_3:+66867823 None:intergenic
CTGTTGTGGAACATTGATTT+TGG 0.228912 3_3:+66867991 None:intergenic
TGGAGCTTCAATGATAATAA+TGG 0.263065 3_3:+66867883 None:intergenic
GCTATACTCAGCCATATCTA+AGG 0.306717 3_3:+66867483 None:intergenic
AAACGTTCAAAGTTGGATAA+AGG 0.314200 3_3:-66867911 Msa0466280:CDS
TCGGTTTATTGCCCCAATTT+TGG 0.319384 3_3:-66867836 Msa0466280:CDS
ATTGGAACTTATATTATTGA+TGG 0.349289 3_3:-66867520 Msa0466280:CDS
GGTCAAAACTGCAGCTAATT+CGG 0.362866 3_3:-66867855 Msa0466280:CDS
CGTTTGTACAAGCCAAAATT+GGG 0.376043 3_3:+66867824 None:intergenic
TTGGTGCCGACCTTCAAATA+TGG 0.377926 3_3:+66868010 None:intergenic
GGAGCTTCAATGATAATAAT+GGG 0.378832 3_3:+66867884 None:intergenic
ATATTTCAAGCCCTTAGATA+TGG 0.400345 3_3:-66867494 Msa0466280:CDS
AACGTTCAAAGTTGGATAAA+GGG 0.431347 3_3:-66867910 Msa0466280:CDS
CGTGACCTCAGCAAATCAAC+TGG 0.460489 3_3:+66867956 None:intergenic
TACCACCAAGCCATATTTGA+AGG 0.463739 3_3:-66868020 Msa0466280:CDS
TGGGCAGTGCGGCGCAGCAT+TGG 0.466372 3_3:-66867538 Msa0466280:CDS
TATCATTGAAGCTCCAAAGA+TGG 0.502814 3_3:-66867876 Msa0466280:CDS
CTTGGTGGTAGAGCAAGAAA+TGG 0.514075 3_3:+66868033 None:intergenic
GGAATATTTGGTATCTGTTG+TGG 0.533879 3_3:+66867977 None:intergenic
TCAATCCTATCATATGAATG+AGG 0.535136 3_3:+66868211 None:intergenic
CAATCCTATCATATGAATGA+GGG 0.550534 3_3:+66868212 None:intergenic
GACCTTCAAATATGGCTTGG+TGG 0.555798 3_3:+66868018 None:intergenic
GATAAAGTTATAAACAGTGT+AGG 0.575222 3_3:-66868076 Msa0466280:CDS
CTATACTCAGCCATATCTAA+GGG 0.575354 3_3:+66867484 None:intergenic
GCCGACCTTCAAATATGGCT+TGG 0.578715 3_3:+66868015 None:intergenic
ATATTCCAGTTGATTTGCTG+AGG 0.581675 3_3:-66867961 Msa0466280:CDS
TGAAGAGAAATCGTCACTGA+AGG 0.602801 3_3:-66868110 Msa0466280:CDS
ACCAAGCCATATTTGAAGGT+CGG 0.616443 3_3:-66868016 Msa0466280:CDS
TTGTACAAACGTGGTGATGT+TGG 0.626789 3_3:-66867812 Msa0466280:CDS
ATCCTATCATATGAATGAGG+GGG 0.635069 3_3:+66868214 None:intergenic
AATCCTATCATATGAATGAG+GGG 0.646986 3_3:+66868213 None:intergenic
ACAAACGTGGTGATGTTGGA+AGG 0.684402 3_3:-66867808 Msa0466280:intron
AAGAAGATGTGTGGGCAGTG+CGG 0.684625 3_3:-66867549 Msa0466280:CDS
GTTTGTACAAGCCAAAATTG+GGG 0.698986 3_3:+66867825 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTCTAAAGATTAATTAAT+TGG + chr3_3:66868029-66868048 None:intergenic 15.0%
!! TTGTCTAAAGATTAATTAAT+TGG + chr3_3:66868029-66868048 None:intergenic 15.0%
!! ATTGGAACTTATATTATTGA+TGG - chr3_3:66868189-66868208 Msa0466280:CDS 20.0%
!! ATTGGAACTTATATTATTGA+TGG - chr3_3:66868189-66868208 Msa0466280:CDS 20.0%
! GATAAAGTTATAAACAGTGT+AGG - chr3_3:66867633-66867652 Msa0466280:intron 25.0%
! TGATGAAAAATTAAGAGATG+AGG + chr3_3:66867934-66867953 None:intergenic 25.0%
! GATAAAGTTATAAACAGTGT+AGG - chr3_3:66867633-66867652 Msa0466280:intron 25.0%
! TGATGAAAAATTAAGAGATG+AGG + chr3_3:66867934-66867953 None:intergenic 25.0%
AATCCTATCATATGAATGAG+GGG + chr3_3:66867499-66867518 None:intergenic 30.0%
CAATCCTATCATATGAATGA+GGG + chr3_3:66867500-66867519 None:intergenic 30.0%
TCAATCCTATCATATGAATG+AGG + chr3_3:66867501-66867520 None:intergenic 30.0%
AGCAAATCAACTGGAATATT+TGG + chr3_3:66867747-66867766 None:intergenic 30.0%
AAACGTTCAAAGTTGGATAA+AGG - chr3_3:66867798-66867817 Msa0466280:intron 30.0%
AACGTTCAAAGTTGGATAAA+GGG - chr3_3:66867799-66867818 Msa0466280:intron 30.0%
GGAGCTTCAATGATAATAAT+GGG + chr3_3:66867828-66867847 None:intergenic 30.0%
TGGAGCTTCAATGATAATAA+TGG + chr3_3:66867829-66867848 None:intergenic 30.0%
AAAAAAGCAAGAAGATGTGT+GGG - chr3_3:66868152-66868171 Msa0466280:CDS 30.0%
ATATTTCAAGCCCTTAGATA+TGG - chr3_3:66868215-66868234 Msa0466280:CDS 30.0%
AATCCTATCATATGAATGAG+GGG + chr3_3:66867499-66867518 None:intergenic 30.0%
CAATCCTATCATATGAATGA+GGG + chr3_3:66867500-66867519 None:intergenic 30.0%
TCAATCCTATCATATGAATG+AGG + chr3_3:66867501-66867520 None:intergenic 30.0%
AGCAAATCAACTGGAATATT+TGG + chr3_3:66867747-66867766 None:intergenic 30.0%
AAACGTTCAAAGTTGGATAA+AGG - chr3_3:66867798-66867817 Msa0466280:intron 30.0%
AACGTTCAAAGTTGGATAAA+GGG - chr3_3:66867799-66867818 Msa0466280:intron 30.0%
GGAGCTTCAATGATAATAAT+GGG + chr3_3:66867828-66867847 None:intergenic 30.0%
TGGAGCTTCAATGATAATAA+TGG + chr3_3:66867829-66867848 None:intergenic 30.0%
AAAAAAGCAAGAAGATGTGT+GGG - chr3_3:66868152-66868171 Msa0466280:CDS 30.0%
ATATTTCAAGCCCTTAGATA+TGG - chr3_3:66868215-66868234 Msa0466280:CDS 30.0%
ATCCTATCATATGAATGAGG+GGG + chr3_3:66867498-66867517 None:intergenic 35.0%
!!! CTGTTGTGGAACATTGATTT+TGG + chr3_3:66867721-66867740 None:intergenic 35.0%
GGAATATTTGGTATCTGTTG+TGG + chr3_3:66867735-66867754 None:intergenic 35.0%
!! ATATTCCAGTTGATTTGCTG+AGG - chr3_3:66867748-66867767 Msa0466280:intron 35.0%
! CGTTTTTCTTGCAACTCTTT+TGG + chr3_3:66867783-66867802 None:intergenic 35.0%
GCAAGAAAAACGTTCAAAGT+TGG - chr3_3:66867791-66867810 Msa0466280:intron 35.0%
TATCATTGAAGCTCCAAAGA+TGG - chr3_3:66867833-66867852 Msa0466280:CDS 35.0%
GTTTGTACAAGCCAAAATTG+GGG + chr3_3:66867887-66867906 None:intergenic 35.0%
CGTTTGTACAAGCCAAAATT+GGG + chr3_3:66867888-66867907 None:intergenic 35.0%
ACGTTTGTACAAGCCAAAAT+TGG + chr3_3:66867889-66867908 None:intergenic 35.0%
!!! AATTTTGGCTTGTACAAACG+TGG - chr3_3:66867888-66867907 Msa0466280:CDS 35.0%
GCAGTAGCAACTAATTGATT+TGG - chr3_3:66867985-66868004 Msa0466280:CDS 35.0%
CAAAAAAGCAAGAAGATGTG+TGG - chr3_3:66868151-66868170 Msa0466280:CDS 35.0%
CTATACTCAGCCATATCTAA+GGG + chr3_3:66868228-66868247 None:intergenic 35.0%
ATCCTATCATATGAATGAGG+GGG + chr3_3:66867498-66867517 None:intergenic 35.0%
!!! CTGTTGTGGAACATTGATTT+TGG + chr3_3:66867721-66867740 None:intergenic 35.0%
GGAATATTTGGTATCTGTTG+TGG + chr3_3:66867735-66867754 None:intergenic 35.0%
!! ATATTCCAGTTGATTTGCTG+AGG - chr3_3:66867748-66867767 Msa0466280:intron 35.0%
! CGTTTTTCTTGCAACTCTTT+TGG + chr3_3:66867783-66867802 None:intergenic 35.0%
GCAAGAAAAACGTTCAAAGT+TGG - chr3_3:66867791-66867810 Msa0466280:intron 35.0%
TATCATTGAAGCTCCAAAGA+TGG - chr3_3:66867833-66867852 Msa0466280:CDS 35.0%
GTTTGTACAAGCCAAAATTG+GGG + chr3_3:66867887-66867906 None:intergenic 35.0%
CGTTTGTACAAGCCAAAATT+GGG + chr3_3:66867888-66867907 None:intergenic 35.0%
ACGTTTGTACAAGCCAAAAT+TGG + chr3_3:66867889-66867908 None:intergenic 35.0%
!!! AATTTTGGCTTGTACAAACG+TGG - chr3_3:66867888-66867907 Msa0466280:CDS 35.0%
GCAGTAGCAACTAATTGATT+TGG - chr3_3:66867985-66868004 Msa0466280:CDS 35.0%
CAAAAAAGCAAGAAGATGTG+TGG - chr3_3:66868151-66868170 Msa0466280:CDS 35.0%
CTATACTCAGCCATATCTAA+GGG + chr3_3:66868228-66868247 None:intergenic 35.0%
ATATGAATGAGGGGGAAAGA+AGG + chr3_3:66867490-66867509 None:intergenic 40.0%
TTCCCCCTCATTCATATGAT+AGG - chr3_3:66867493-66867512 Msa0466280:CDS 40.0%
TGAAGAGAAATCGTCACTGA+AGG - chr3_3:66867599-66867618 Msa0466280:intron 40.0%
TACCACCAAGCCATATTTGA+AGG - chr3_3:66867689-66867708 Msa0466280:intron 40.0%
ACCAAGCCATATTTGAAGGT+CGG - chr3_3:66867693-66867712 Msa0466280:intron 40.0%
GGTCAAAACTGCAGCTAATT+CGG - chr3_3:66867854-66867873 Msa0466280:CDS 40.0%
! TCGGTTTATTGCCCCAATTT+TGG - chr3_3:66867873-66867892 Msa0466280:CDS 40.0%
! TTGTACAAACGTGGTGATGT+TGG - chr3_3:66867897-66867916 Msa0466280:CDS 40.0%
GCTATACTCAGCCATATCTA+AGG + chr3_3:66868229-66868248 None:intergenic 40.0%
ATATGAATGAGGGGGAAAGA+AGG + chr3_3:66867490-66867509 None:intergenic 40.0%
TTCCCCCTCATTCATATGAT+AGG - chr3_3:66867493-66867512 Msa0466280:CDS 40.0%
TGAAGAGAAATCGTCACTGA+AGG - chr3_3:66867599-66867618 Msa0466280:intron 40.0%
TACCACCAAGCCATATTTGA+AGG - chr3_3:66867689-66867708 Msa0466280:intron 40.0%
ACCAAGCCATATTTGAAGGT+CGG - chr3_3:66867693-66867712 Msa0466280:intron 40.0%
GGTCAAAACTGCAGCTAATT+CGG - chr3_3:66867854-66867873 Msa0466280:CDS 40.0%
! TCGGTTTATTGCCCCAATTT+TGG - chr3_3:66867873-66867892 Msa0466280:CDS 40.0%
! TTGTACAAACGTGGTGATGT+TGG - chr3_3:66867897-66867916 Msa0466280:CDS 40.0%
GCTATACTCAGCCATATCTA+AGG + chr3_3:66868229-66868248 None:intergenic 40.0%
! CTTGGTGGTAGAGCAAGAAA+TGG + chr3_3:66867679-66867698 None:intergenic 45.0%
! GACCTTCAAATATGGCTTGG+TGG + chr3_3:66867694-66867713 None:intergenic 45.0%
!! TTGGTGCCGACCTTCAAATA+TGG + chr3_3:66867702-66867721 None:intergenic 45.0%
!! GCTGCAGTTTTGACCATCTT+TGG + chr3_3:66867849-66867868 None:intergenic 45.0%
! ACAAACGTGGTGATGTTGGA+AGG - chr3_3:66867901-66867920 Msa0466280:CDS 45.0%
! CTTGGTGGTAGAGCAAGAAA+TGG + chr3_3:66867679-66867698 None:intergenic 45.0%
! GACCTTCAAATATGGCTTGG+TGG + chr3_3:66867694-66867713 None:intergenic 45.0%
!! TTGGTGCCGACCTTCAAATA+TGG + chr3_3:66867702-66867721 None:intergenic 45.0%
!! GCTGCAGTTTTGACCATCTT+TGG + chr3_3:66867849-66867868 None:intergenic 45.0%
! ACAAACGTGGTGATGTTGGA+AGG - chr3_3:66867901-66867920 Msa0466280:CDS 45.0%
GCCGACCTTCAAATATGGCT+TGG + chr3_3:66867697-66867716 None:intergenic 50.0%
CGTGACCTCAGCAAATCAAC+TGG + chr3_3:66867756-66867775 None:intergenic 50.0%
AAGAAGATGTGTGGGCAGTG+CGG - chr3_3:66868160-66868179 Msa0466280:CDS 50.0%
GCCGACCTTCAAATATGGCT+TGG + chr3_3:66867697-66867716 None:intergenic 50.0%
CGTGACCTCAGCAAATCAAC+TGG + chr3_3:66867756-66867775 None:intergenic 50.0%
AAGAAGATGTGTGGGCAGTG+CGG - chr3_3:66868160-66868179 Msa0466280:CDS 50.0%
TGGGCAGTGCGGCGCAGCAT+TGG - chr3_3:66868171-66868190 Msa0466280:CDS 70.0%
TGGGCAGTGCGGCGCAGCAT+TGG - chr3_3:66868171-66868190 Msa0466280:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 66867480 66868251 66867480 ID=Msa0466280;Name=Msa0466280
chr3_3 mRNA 66867480 66868251 66867480 ID=Msa0466280-mRNA-1;Parent=Msa0466280;Name=Msa0466280-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|1|0|1|1|1|2|0|183
chr3_3 exon 66867809 66868251 66867809 ID=Msa0466280-mRNA-1:exon:15271;Parent=Msa0466280-mRNA-1
chr3_3 exon 66867480 66867588 66867480 ID=Msa0466280-mRNA-1:exon:15270;Parent=Msa0466280-mRNA-1
chr3_3 CDS 66867809 66868251 66867809 ID=Msa0466280-mRNA-1:cds;Parent=Msa0466280-mRNA-1
chr3_3 CDS 66867480 66867588 66867480 ID=Msa0466280-mRNA-1:cds;Parent=Msa0466280-mRNA-1
Gene Sequence

>Msa0466280

ATGTTAACCTTCTTTCCCCCTCATTCATATGATAGGATTGAATCTCACTTCATTCAGCTCATCGTCAAAGATGTTTCTAAAAAAGTTAATACAAAACTTGTTGAAATTGAGAAAAAGTATGAAGAGAAATCGTCACTGAAGGTCACGCTAGCTGATAAAGTTATAAACAGTGTAGGAAAAGCTATGTCTAATTTGTCCATTTCTTGCTCTACCACCAAGCCATATTTGAAGGTCGGCACCAAAATCAATGTTCCACAACAGATACCAAATATTCCAGTTGATTTGCTGAGGTCACGTGAACCAAAAGAGTTGCAAGAAAAACGTTCAAAGTTGGATAAAGGGTCTCCCATTATTATCATTGAAGCTCCAAAGATGGTCAAAACTGCAGCTAATTCGGTTTATTGCCCCAATTTTGGCTTGTACAAACGTGGTGATGTTGGAAGAATTGTTTCAAAAAAGCAAGAAGATGTGTGGGCAGTGCGGCGCAGCATTGGAACTTATATTATTGATGGCAAATATTTCAAGCCCTTAGATATGGCTGAGTATAGCTAG

Protein sequence

>Msa0466280

MLTFFPPHSYDRIESHFIQLIVKDVSKKVNTKLVEIEKKYEEKSSLKVTLADKVINSVGKAMSNLSISCSTTKPYLKVGTKINVPQQIPNIPVDLLRSREPKELQEKRSKLDKGSPIIIIEAPKMVKTAANSVYCPNFGLYKRGDVGRIVSKKQEDVWAVRRSIGTYIIDGKYFKPLDMAEYS*