AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0404130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404130 MtrunA17_Chr3g0132461 93.333 345 22 1 1 345 1 344 0.0 547
Msa0404130 MtrunA17_Chr3g0115141 90.860 186 17 0 346 531 25 210 1.47e-120 353
Msa0404130 MtrunA17_Chr2g0286171 84.239 184 29 0 348 531 29 212 2.29e-107 319
Msa0404130 MtrunA17_Chr4g0003361 67.797 177 53 2 358 531 30 205 2.94e-77 241
Msa0404130 MtrunA17_Chr5g0426721 48.747 359 127 6 1 341 1 320 3.39e-76 243
Msa0404130 MtrunA17_Chr8g0343954 61.111 180 67 1 355 531 30 209 2.90e-73 231
Msa0404130 MtrunA17_Chr8g0344041 63.536 181 62 2 355 531 33 213 5.62e-72 228
Msa0404130 MtrunA17_Chr8g0343934 60.000 180 69 1 355 531 30 209 1.90e-71 227
Msa0404130 MtrunA17_Chr8g0343961 59.887 177 68 1 358 531 33 209 6.30e-70 223
Msa0404130 MtrunA17_Chr8g0344031 63.277 177 62 1 358 531 35 211 2.79e-67 216
Msa0404130 MtrunA17_Chr2g0301611 56.667 180 75 1 355 531 30 209 1.01e-65 212
Msa0404130 MtrunA17_Chr7g0217811 57.222 180 74 1 355 531 30 209 1.47e-64 209
Msa0404130 MtrunA17_Chr2g0302171 56.667 180 75 1 355 531 30 209 2.32e-64 208
Msa0404130 MtrunA17_Chr2g0302541 55.000 180 78 1 355 531 30 209 8.89e-63 204
Msa0404130 MtrunA17_Chr4g0012651 55.556 180 77 1 355 531 30 209 2.68e-62 203
Msa0404130 MtrunA17_Chr2g0313961 54.190 179 79 1 355 530 30 208 5.57e-62 202
Msa0404130 MtrunA17_Chr3g0084531 54.444 180 79 1 355 531 30 209 6.60e-62 202
Msa0404130 MtrunA17_Chr8g0344021 54.082 196 86 2 340 531 17 212 1.10e-61 201
Msa0404130 MtrunA17_Chr2g0297241 53.073 179 81 1 355 530 30 208 6.19e-61 199
Msa0404130 MtrunA17_Chr5g0411961 55.429 175 75 1 360 531 35 209 3.06e-60 197
Msa0404130 MtrunA17_Chr2g0297231 53.107 177 80 1 355 528 30 206 7.23e-60 196
Msa0404130 MtrunA17_Chr3g0084511 53.672 177 79 1 358 531 32 208 1.45e-59 196
Msa0404130 MtrunA17_Chr3g0084521 53.672 177 79 1 358 531 32 208 2.00e-59 195
Msa0404130 MtrunA17_Chr8g0344051 51.099 182 84 3 355 531 31 212 1.03e-57 191
Msa0404130 MtrunA17_Chr8g0343911 50.562 178 83 3 359 531 31 208 8.66e-56 186
Msa0404130 MtrunA17_Chr8g0343921 46.040 202 104 4 333 531 8 207 4.79e-51 173
Msa0404130 MtrunA17_Chr2g0300561 43.646 181 97 3 356 531 30 210 1.48e-49 169
Msa0404130 MtrunA17_Chr2g0300581 43.646 181 97 3 356 531 30 210 5.75e-49 168
Msa0404130 MtrunA17_Chr2g0300591 43.646 181 97 3 356 531 30 210 2.03e-48 166
Msa0404130 MtrunA17_Chr3g0084551 47.701 174 82 2 355 525 30 197 2.58e-44 156
Msa0404130 MtrunA17_Chr1g0189841 40.000 150 83 2 366 508 47 196 3.47e-26 106
Msa0404130 MtrunA17_Chr1g0193421 34.682 173 106 4 360 525 41 213 7.17e-24 99.8
Msa0404130 MtrunA17_Chr1g0178711 33.939 165 103 4 365 524 55 218 5.87e-23 97.4
Msa0404130 MtrunA17_Chr1g0210761 34.118 170 106 4 364 528 40 208 5.98e-22 94.4
Msa0404130 MtrunA17_Chr6g0449771 39.189 148 83 3 362 503 43 189 1.17e-21 93.6
Msa0404130 MtrunA17_Chr1g0193671 34.911 169 103 4 363 524 43 211 1.42e-21 93.2
Msa0404130 MtrunA17_Chr7g0271211 35.882 170 102 4 363 525 40 209 1.61e-21 93.2
Msa0404130 MtrunA17_Chr8g0335851 37.594 133 79 2 363 491 48 180 1.93e-21 93.2
Msa0404130 MtrunA17_Chr6g0449791 37.821 156 83 4 343 491 30 178 3.03e-21 92.0
Msa0404130 MtrunA17_Chr6g0449761 38.514 148 84 3 362 503 43 189 4.46e-21 92.4
Msa0404130 MtrunA17_Chr6g0449751 38.889 144 81 3 366 503 47 189 6.43e-21 91.7
Msa0404130 MtrunA17_Chr6g0449731 38.889 144 81 3 366 503 47 189 8.75e-21 91.3
Msa0404130 MtrunA17_Chr3g0084501 48.387 93 39 2 338 427 19 105 1.33e-20 87.0
Msa0404130 MtrunA17_Chr2g0292831 36.585 164 94 4 366 522 42 202 4.58e-20 89.0
Msa0404130 MtrunA17_Chr4g0007041 37.241 145 83 3 366 503 47 190 4.76e-20 89.0
Msa0404130 MtrunA17_Chr4g0026201 34.066 182 112 4 352 528 28 206 5.53e-20 88.6
Msa0404130 MtrunA17_Chr2g0285871 36.552 145 84 3 366 503 47 190 9.98e-20 88.2
Msa0404130 MtrunA17_Chr4g0007051 36.552 145 84 3 366 503 47 190 1.24e-19 87.8
Msa0404130 MtrunA17_Chr4g0007031 36.552 145 84 3 366 503 47 190 1.34e-19 87.8
Msa0404130 MtrunA17_Chr5g0419971 36.552 145 84 3 366 503 47 190 1.34e-19 87.8
Msa0404130 MtrunA17_Chr5g0419981 36.552 145 84 3 366 503 47 190 1.34e-19 87.8
Msa0404130 MtrunA17_Chr4g0026191 33.516 182 113 4 352 528 28 206 1.72e-19 87.0
Msa0404130 MtrunA17_Chr2g0321591 34.426 183 109 6 352 528 28 205 2.50e-19 86.7
Msa0404130 MtrunA17_Chr5g0414971 36.111 144 86 4 363 500 47 190 2.63e-19 87.0
Msa0404130 MtrunA17_Chr2g0292901 33.333 171 105 4 366 527 43 213 3.80e-19 86.3
Msa0404130 MtrunA17_Chr3g0130241 33.775 151 88 3 358 496 98 248 3.83e-19 87.8
Msa0404130 MtrunA17_Chr2g0321581 33.880 183 110 6 352 528 28 205 8.55e-19 85.1
Msa0404130 MtrunA17_Chr2g0327651 40.594 101 58 1 367 465 7 107 2.98e-18 80.9
Msa0404130 MtrunA17_Chr2g0321601 32.240 183 113 7 352 528 27 204 4.36e-18 82.8
Msa0404130 MtrunA17_Chr1g0156131 32.749 171 106 4 366 527 39 209 4.84e-18 82.8
Msa0404130 MtrunA17_Chr2g0292911 38.462 117 65 3 366 475 7 123 7.08e-17 77.4
Msa0404130 MtrunA17_Chr6g0449781 35.762 151 84 4 343 487 30 173 9.12e-16 76.6
Msa0404130 MtrunA17_Chr2g0292861 31.395 172 108 5 366 527 7 178 1.40e-15 75.1
Msa0404130 MtrunA17_Chr2g0292841 31.395 172 108 5 366 527 7 178 2.03e-14 71.6
Msa0404130 MtrunA17_Chr5g0426661 45.395 152 46 2 192 341 45 161 4.49e-14 70.5
Msa0404130 MtrunA17_Chr8g0337301 79.487 39 6 1 99 137 1 37 2.24e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404130 AT4G09060.1 41.071 336 157 6 15 348 45 341 3.36e-60 202
Msa0404130 AT4G09060.2 38.889 360 157 7 15 348 45 367 6.51e-57 194
Msa0404130 AT1G14680.1 42.336 274 133 6 15 285 29 280 2.06e-54 185
Msa0404130 AT1G72610.1 46.154 182 93 3 355 531 27 208 5.80e-52 176
Msa0404130 AT5G20630.1 44.693 179 94 3 357 530 32 210 2.63e-46 161
Msa0404130 AT5G39190.1 37.572 173 98 5 366 528 48 220 2.68e-26 107
Msa0404130 AT5G39160.3 36.667 180 104 5 359 528 37 216 3.27e-26 107
Msa0404130 AT5G39160.1 36.994 173 99 5 366 528 48 220 7.51e-26 105
Msa0404130 AT5G39130.1 37.209 172 98 5 366 527 48 219 1.83e-25 104
Msa0404130 AT5G38960.1 37.791 172 97 4 366 527 51 222 5.44e-25 103
Msa0404130 AT1G09560.1 37.209 172 101 4 363 527 44 215 6.27e-25 103
Msa0404130 AT1G02335.1 34.911 169 103 4 363 524 44 212 1.06e-24 102
Msa0404130 AT4G14630.1 36.047 172 100 4 366 527 51 222 1.51e-24 102
Msa0404130 AT5G39110.1 36.628 172 99 4 366 527 49 220 1.95e-24 102
Msa0404130 AT3G04200.1 35.028 177 105 4 362 528 47 223 1.03e-23 100
Msa0404130 AT5G39190.2 35.484 155 94 2 380 528 44 198 1.40e-23 99.0
Msa0404130 AT5G39180.1 36.628 172 99 4 366 527 48 219 1.41e-23 99.4
Msa0404130 AT5G61750.1 38.806 134 77 3 363 491 41 174 3.01e-23 98.2
Msa0404130 AT5G39150.1 36.628 172 99 4 366 527 48 219 3.29e-23 98.6
Msa0404130 AT5G39120.1 36.628 172 99 4 366 527 48 219 4.54e-23 98.2
Msa0404130 AT5G39160.2 34.839 155 95 2 380 528 44 198 4.61e-23 97.4
Msa0404130 AT5G38940.1 40.000 145 80 3 366 503 51 195 1.01e-22 97.1
Msa0404130 AT3G05950.1 36.047 172 100 4 366 527 50 221 1.30e-22 97.1
Msa0404130 AT3G04190.1 34.884 172 102 4 366 527 50 221 1.44e-22 96.7
Msa0404130 AT5G38940.2 40.000 145 80 3 366 503 75 219 1.66e-22 97.1
Msa0404130 AT5G26700.1 37.288 177 101 5 355 524 33 206 6.42e-22 94.7
Msa0404130 AT5G38910.1 40.816 147 76 4 366 503 49 193 1.12e-21 94.0
Msa0404130 AT3G05930.1 34.682 173 105 4 363 527 43 215 2.78e-21 92.8
Msa0404130 AT3G04150.2 29.545 176 114 4 366 531 63 238 1.27e-20 91.7
Msa0404130 AT5G38930.1 37.931 145 83 3 366 503 51 195 1.46e-20 90.9
Msa0404130 AT3G62020.2 32.948 173 108 5 363 527 14 186 1.60e-20 90.1
Msa0404130 AT3G04150.1 29.545 176 114 4 366 531 50 225 2.15e-20 90.5
Msa0404130 AT3G04170.1 32.948 173 103 5 366 528 50 219 2.47e-20 90.5
Msa0404130 AT3G62020.1 32.948 173 108 5 363 527 43 215 2.71e-20 90.1
Msa0404130 AT1G18980.1 34.118 170 107 4 363 527 49 218 5.36e-20 89.4
Msa0404130 AT3G04180.1 34.104 173 104 4 366 528 50 222 7.76e-20 89.0
Msa0404130 AT3G10080.1 33.728 169 106 4 365 528 53 220 2.42e-19 87.4
Msa0404130 AT3G10080.2 33.728 169 106 4 365 528 124 291 3.27e-19 88.6
Msa0404130 AT1G10460.1 36.054 147 87 5 366 505 45 191 4.90e-19 86.3
Msa0404130 AT1G18970.1 31.176 170 112 4 363 527 49 218 4.94e-17 80.5
Msa0404130 AT1G74820.1 36.752 117 71 3 362 475 55 171 3.05e-14 72.8

Find 114 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACTGGGCTACTTGTTT+TGG 0.124089 3_2:+34837653 None:intergenic
AGGGAAGCGTTGATGGAATT+TGG 0.124394 3_2:-34840082 Msa0404130:CDS
GTTGGAGGAGTTGATCTTTC+TGG 0.156099 3_2:+34840337 None:intergenic
ACACTAAAAGGGTTTGAATT+AGG 0.165118 3_2:+34840563 None:intergenic
TGATTCTACATATGCATTTC+TGG 0.168736 3_2:-34838668 Msa0404130:CDS
TTGAATTGGGTGACACTAAA+AGG 0.199068 3_2:+34840551 None:intergenic
TTTCTGAAGCGACTTGATTT+TGG 0.213795 3_2:+34838359 None:intergenic
GTTGGATGAAGGGCAGAATT+TGG 0.224805 3_2:-34840134 Msa0404130:CDS
TTCAAAAGAATTAGTTGAAT+TGG 0.231495 3_2:+34840537 None:intergenic
TCTTAAGCTTGGTGATATTA+TGG 0.244582 3_2:-34836208 Msa0404130:CDS
TTCCTTTCCTTCTGCTCCAC+TGG 0.254723 3_2:+34837639 None:intergenic
GAATTTATCTGACTCTCTTT+TGG 0.280362 3_2:-34838713 Msa0404130:CDS
ACAAAATGCATTAGTCCTTT+AGG 0.284163 3_2:+34836180 None:intergenic
AGAGTAGACATTGGTGTAAA+TGG 0.295168 3_2:-34836336 Msa0404130:CDS
AGCATTGAAGAAATTTGATT+TGG 0.298688 3_2:-34840191 Msa0404130:CDS
ACAGGCTCAGTCGCGTGTTT+TGG 0.300454 3_2:-34840035 Msa0404130:CDS
CACTAAAAGGGTTTGAATTA+GGG 0.305080 3_2:+34840564 None:intergenic
AAATCTTTGTTTATGTTTCC+AGG 0.307282 3_2:+34836429 None:intergenic
GTATGTTTCTCGCCGGAGTT+GGG 0.324190 3_2:+34837612 None:intergenic
TTGAAAACGAGTCATGTAAA+AGG 0.326625 3_2:-34838636 Msa0404130:CDS
GGTATGTTTCTCGCCGGAGT+TGG 0.339529 3_2:+34837611 None:intergenic
TCAAAAGAATTAGTTGAATT+GGG 0.344145 3_2:+34840538 None:intergenic
CTACAGTCTGTGGAGTTATA+TGG 0.352021 3_2:+34837284 None:intergenic
GTATGTTTCTCGCCTGAACT+TGG 0.360266 3_2:+34838113 None:intergenic
GTTGATCTTTCTGGTTCAAT+TGG 0.361089 3_2:+34840346 None:intergenic
TTATATGGTAAGGGTGATAA+TGG 0.368869 3_2:+34837299 None:intergenic
TTGCGTTGTATGAACATGAT+AGG 0.368898 3_2:-34840102 Msa0404130:CDS
TAATTCAATGACAGATGCTT+TGG 0.376573 3_2:+34836392 None:intergenic
CTTCTTCTACATGCACAGTT+GGG 0.379853 3_2:-34838741 Msa0404130:CDS
GGGAAGCGTTGATGGAATTT+GGG 0.384313 3_2:-34840081 Msa0404130:CDS
TCCTTTCCTTCTGCTCCACT+GGG 0.387253 3_2:+34837640 None:intergenic
TTCACAAATTAAGAAGCTTA+AGG 0.390554 3_2:-34836019 Msa0404130:CDS
AAGCTTAAGGCTCCGTTTAA+CGG 0.392227 3_2:-34836006 Msa0404130:CDS
GGGATGATGAATTTCAAATT+CGG 0.394019 3_2:+34840484 None:intergenic
GTGTTCTCTGGCTTTGTACC+TGG 0.400296 3_2:-34836447 Msa0404130:CDS
ACATGATAGGGAAGCGTTGA+TGG 0.413511 3_2:-34840089 Msa0404130:CDS
GAAAGGGATGACCCAAGTTC+AGG 0.419002 3_2:-34838125 Msa0404130:CDS
ATCTAACTCGCCTTGGAAGA+AGG 0.424488 3_2:+34838083 None:intergenic
TTTGTTGTTAACTCTGGTGC+TGG 0.433028 3_2:-34836162 Msa0404130:CDS
GGGAAATCCTCCTAGTGAAA+TGG 0.435106 3_2:-34840454 Msa0404130:CDS
TCCTAGCCAGTGCCGTTAAA+CGG 0.436803 3_2:+34835994 None:intergenic
GTCTGTGGAGTTATATGGTA+AGG 0.437588 3_2:+34837289 None:intergenic
GGATAGCCTGAAGGAGTGTC+TGG 0.445743 3_2:+34836498 None:intergenic
TCTTCTTCTACATGCACAGT+TGG 0.448220 3_2:-34838742 Msa0404130:CDS
GATCTTTCTGGTTCAATTGG+AGG 0.449109 3_2:+34840349 None:intergenic
GGCTGCTGAGTATGTGGAAC+TGG 0.451776 3_2:-34838615 Msa0404130:intron
CCGAAACCTTGTGGCGTTGG+AGG 0.452249 3_2:+34840322 None:intergenic
TCAATTGGAGGAGAAGAGAA+AGG 0.453678 3_2:+34840361 None:intergenic
TGCAACTGAATTACTAATCA+TGG 0.460173 3_2:-34836286 Msa0404130:CDS
TGAATTGGGTGACACTAAAA+GGG 0.462597 3_2:+34840552 None:intergenic
CTTTGATATAAACTTCTGTA+AGG 0.462757 3_2:+34836232 None:intergenic
AATTTGTAGCTTAGAGCCAT+TGG 0.470537 3_2:-34838406 Msa0404130:intron
TATATCAAAGATCTTAAGCT+TGG 0.472626 3_2:-34836219 Msa0404130:CDS
CGCCTTGGCAGATCGGCTGC+TGG 0.478298 3_2:+34837590 None:intergenic
CGCCTTGGAAGAAGGGCTGC+TGG 0.481985 3_2:+34838091 None:intergenic
ATTAGTAATTCAGTTGCATC+GGG 0.487113 3_2:+34836291 None:intergenic
TGTTGGACAAGGTGAACAAA+AGG 0.491432 3_2:-34838166 Msa0404130:CDS
TGTCAGAAATTGTTGGATGA+AGG 0.493834 3_2:-34840145 Msa0404130:CDS
AAACCGAAACCTTGTGGCGT+TGG 0.495159 3_2:+34840319 None:intergenic
ATTGAATTACCAAGTCTCAA+TGG 0.496291 3_2:-34836378 Msa0404130:CDS
TCCGTTTAACGGCACTGGCT+AGG 0.501196 3_2:-34835995 None:intergenic
AGCTGATGAACGTGAACGAC+AGG 0.501328 3_2:-34840053 Msa0404130:CDS
GTTCAAGGTAAAGTTACAGC+TGG 0.503847 3_2:-34836264 Msa0404130:CDS
GAATTACTAATCATGGTTCA+AGG 0.507381 3_2:-34836279 Msa0404130:CDS
GAAAGGAACCACCCCAACTC+CGG 0.512929 3_2:-34837624 Msa0404130:CDS
GATTAGTAATTCAGTTGCAT+CGG 0.513383 3_2:+34836290 None:intergenic
AAGTAGCCCAGTGGAGCAGA+AGG 0.514228 3_2:-34837646 Msa0404130:CDS
TAATCAAGATACTGAAAGCT+AGG 0.521850 3_2:+34836102 None:intergenic
TCTAACTCGCCTTGGAAGAA+GGG 0.523478 3_2:+34838084 None:intergenic
GTTTGATGAAAACAATATGT+TGG 0.525673 3_2:-34838183 Msa0404130:CDS
AAGGCTCCGTTTAACGGCAC+TGG 0.525904 3_2:-34836000 Msa0404130:CDS
TGCGTTGTATGAACATGATA+GGG 0.532202 3_2:-34840101 Msa0404130:CDS
AACAATCCAGACACTCCTTC+AGG 0.539363 3_2:-34836504 Msa0404130:CDS
TCTAACTCGCCTTGGCAGAT+CGG 0.540039 3_2:+34837583 None:intergenic
GCCCAGTGGAGCAGAAGGAA+AGG 0.542432 3_2:-34837641 Msa0404130:CDS
TATGTTTCTCGCCTGAACTT+GGG 0.543626 3_2:+34838114 None:intergenic
TTCTTCTACATGCACAGTTG+GGG 0.545979 3_2:-34838740 Msa0404130:CDS
CAAGCATCGAAACATTTCAA+TGG 0.548609 3_2:-34839966 Msa0404130:intron
CAAGTCGCTTCAGAAAGATA+AGG 0.550339 3_2:-34838352 Msa0404130:CDS
TGAAGGGCAGAATTTGGAAA+AGG 0.552431 3_2:-34840128 Msa0404130:CDS
AACACAAAATCATCAACTGT+TGG 0.553601 3_2:+34836465 None:intergenic
TCTTCACAAAGCTGAGGAGG+AGG 0.555115 3_2:-34838313 Msa0404130:intron
GTCAGAAATTGTTGGATGAA+GGG 0.555361 3_2:-34840144 Msa0404130:CDS
ACAAGGTGAACAAAAGGCTA+TGG 0.555485 3_2:-34838160 Msa0404130:CDS
GTAAAAGGCTGCTGAGTATG+TGG 0.556401 3_2:-34838621 Msa0404130:CDS
GACAAAACTCTATGAACCAA+TGG 0.560149 3_2:+34838390 None:intergenic
TCAACTGTTGGTGATTTGCA+TGG 0.567977 3_2:+34836477 None:intergenic
GTCTGTGCAATTATAGAAGT+AGG 0.569773 3_2:+34836057 None:intergenic
GTTTCTCGCCGGAGTTGGGG+TGG 0.571301 3_2:+34837616 None:intergenic
TACCAGCAGCCCTTCTTCCA+AGG 0.572211 3_2:-34838093 Msa0404130:intron
CTTACGTATTCTACAGTCTG+TGG 0.573268 3_2:+34837256 None:intergenic
ATGGAAGAAATGCAAGGAAA+GGG 0.574271 3_2:-34838141 Msa0404130:CDS
TACCAGCAGCCGATCTGCCA+AGG 0.583286 3_2:-34837592 Msa0404130:intron
TGTGGATATTCTACAGTCTG+TGG 0.588698 3_2:+34837274 None:intergenic
TATGGAAGAAATGCAAGGAA+AGG 0.589608 3_2:-34838142 Msa0404130:CDS
TAATCGTCCATTTCACTAGG+AGG 0.593363 3_2:+34840447 None:intergenic
TATGTTTCTCGCCGGAGTTG+GGG 0.594478 3_2:+34837613 None:intergenic
TAATCTTCACAAAGCTGAGG+AGG 0.595349 3_2:-34838316 Msa0404130:CDS
TCTGTGGAGTTATATGGTAA+GGG 0.601908 3_2:+34837290 None:intergenic
ATGTAATCGTCCATTTCACT+AGG 0.603612 3_2:+34840444 None:intergenic
TGAGCTACTATAAGTAGCAA+AGG 0.608217 3_2:+34836128 None:intergenic
TCAACTCCTCCAACGCCACA+AGG 0.620149 3_2:-34840328 Msa0404130:intron
ACATCGAGTCCATTGAGACT+TGG 0.620421 3_2:+34836369 None:intergenic
GCATCGGGATGAGTATGCAT+CGG 0.629161 3_2:+34836306 None:intergenic
GCTGCTGGTATGTTTCTCGC+CGG 0.638632 3_2:+34837605 None:intergenic
GGAGAATCAGAGACTTGCGT+CGG 0.645431 3_2:-34840170 Msa0404130:CDS
GTCGGCGTGTCAGAAATTGT+TGG 0.645734 3_2:-34840152 Msa0404130:CDS
AAGGCTATGGAAGAAATGCA+AGG 0.653172 3_2:-34838147 Msa0404130:CDS
AATTAATCTTCACAAAGCTG+AGG 0.656091 3_2:-34838319 Msa0404130:CDS
CAATGGACTCGATGTCTCTG+CGG 0.663400 3_2:-34836361 Msa0404130:CDS
TCTGCGGCAAGAGTAGACAT+TGG 0.691986 3_2:-34836345 Msa0404130:CDS
TCCCAAAACAAGTAGCCCAG+TGG 0.700178 3_2:-34837655 Msa0404130:CDS
GATTTGCATGGATAGCCTGA+AGG 0.717386 3_2:+34836489 None:intergenic
TGAAAACAATATGTTGGACA+AGG 0.733157 3_2:-34838177 Msa0404130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTATCATAAAAAAAG+AGG + chr3_2:34836336-34836355 None:intergenic 10.0%
!! TAATAATTTGAGTAATAATT+CGG - chr3_2:34836779-34836798 Msa0404130:intron 10.0%
!!! AAATTTTATCAAATCAATAA+AGG - chr3_2:34837090-34837109 Msa0404130:intron 10.0%
!! AAACAATATTTCAAAAATAG+GGG + chr3_2:34836699-34836718 None:intergenic 15.0%
!! CAAACAATATTTCAAAAATA+GGG + chr3_2:34836700-34836719 None:intergenic 15.0%
!! TCAAACAATATTTCAAAAAT+AGG + chr3_2:34836701-34836720 None:intergenic 15.0%
!! AAAAATAGATTATAATCACA+GGG + chr3_2:34837535-34837554 None:intergenic 15.0%
!! AAAAAATAGATTATAATCAC+AGG + chr3_2:34837536-34837555 None:intergenic 15.0%
!! TTACCATATTAATATGTAAA+GGG + chr3_2:34837788-34837807 None:intergenic 15.0%
!!! TTAAATTATTTTTGACAAGA+AGG - chr3_2:34838569-34838588 Msa0404130:intron 15.0%
!!! GTCATATTTTTTACTTTAAT+TGG - chr3_2:34839645-34839664 Msa0404130:intron 15.0%
!! TCAAAAGAATTAGTTGAATT+GGG + chr3_2:34836090-34836109 None:intergenic 20.0%
!! TTCAAAAGAATTAGTTGAAT+TGG + chr3_2:34836091-34836110 None:intergenic 20.0%
!!! TTGACTTAATTTTGTTGTTT+TGG - chr3_2:34836383-34836402 Msa0404130:CDS 20.0%
!! AACAATATTTCAAAAATAGG+GGG + chr3_2:34836698-34836717 None:intergenic 20.0%
!! TCTTAAATCATCAAAATAAG+TGG + chr3_2:34836732-34836751 None:intergenic 20.0%
!! TACTATATAGATTAGTAGTT+TGG - chr3_2:34836977-34836996 Msa0404130:intron 20.0%
!! ATTTCAAAATAGTAACCTAT+GGG + chr3_2:34837408-34837427 None:intergenic 20.0%
!! AATTTCAAAATAGTAACCTA+TGG + chr3_2:34837409-34837428 None:intergenic 20.0%
!! TTTCCCTTTACATATTAATA+TGG - chr3_2:34837782-34837801 Msa0404130:intron 20.0%
!! GTTACCATATTAATATGTAA+AGG + chr3_2:34837789-34837808 None:intergenic 20.0%
!!! TTGTAAATTATAGTTCCTTT+TGG - chr3_2:34837814-34837833 Msa0404130:intron 20.0%
!! GAATAATCACTAAAGATTAA+TGG + chr3_2:34838071-34838090 None:intergenic 20.0%
!! GAAATGGTTCAAAAAAATTA+GGG + chr3_2:34838354-34838373 None:intergenic 20.0%
!! TGAAATGGTTCAAAAAAATT+AGG + chr3_2:34838355-34838374 None:intergenic 20.0%
!!! TGATTTTCTCAAATTATTAG+TGG + chr3_2:34838759-34838778 None:intergenic 20.0%
!! TTCAGTATAATTATTTGTGT+CGG - chr3_2:34838784-34838803 Msa0404130:intron 20.0%
!! AAATATACATATAATCCATC+AGG + chr3_2:34839181-34839200 None:intergenic 20.0%
!! CCAATATAAATTGCATATTA+CGG - chr3_2:34839826-34839845 Msa0404130:intron 20.0%
!!! CATCTTTGAAAATTGAATTT+GGG + chr3_2:34840002-34840021 None:intergenic 20.0%
!!! TCATCTTTGAAAATTGAATT+TGG + chr3_2:34840003-34840022 None:intergenic 20.0%
!!! TTGAATTAGGGTTTTTGATT+GGG + chr3_2:34836052-34836071 None:intergenic 25.0%
!! TTTGAATTAGGGTTTTTGAT+TGG + chr3_2:34836053-34836072 None:intergenic 25.0%
!!! AAGGTATTTTTGTGATTCTT+GGG + chr3_2:34836233-34836252 None:intergenic 25.0%
!! AGCATTGAAGAAATTTGATT+TGG - chr3_2:34836434-34836453 Msa0404130:CDS 25.0%
! TAATTTGAGTAATAATTCGG+TGG - chr3_2:34836782-34836801 Msa0404130:intron 25.0%
! TTCCTTCAAAACAAAGATAA+CGG + chr3_2:34837051-34837070 None:intergenic 25.0%
! TAAAGGAATCAAAATCAGTT+TGG - chr3_2:34837107-34837126 Msa0404130:intron 25.0%
!!! GATTTTAGCAATTAAGAGAA+TGG - chr3_2:34837289-34837308 Msa0404130:CDS 25.0%
! TGTAAACTAGAAAATGTAAG+AGG - chr3_2:34837357-34837376 Msa0404130:CDS 25.0%
!!! AAAATGTAAGAGGAGTATTT+TGG - chr3_2:34837367-34837386 Msa0404130:CDS 25.0%
! TGAAATTTCATTATCTGATG+TGG - chr3_2:34837422-34837441 Msa0404130:intron 25.0%
!!! AATCTATTTTTTGTTTCCAG+TGG - chr3_2:34837544-34837563 Msa0404130:intron 25.0%
! ATTAATGGCAAGATGAAAAA+TGG + chr3_2:34838056-34838075 None:intergenic 25.0%
!! AACCATTTCATTTCTTTTGA+TGG - chr3_2:34838365-34838384 Msa0404130:CDS 25.0%
! AACCATCAAAAGAAATGAAA+TGG + chr3_2:34838370-34838389 None:intergenic 25.0%
! GTTTGATGAAAACAATATGT+TGG - chr3_2:34838442-34838461 Msa0404130:intron 25.0%
! AGATACACATGATAACTATT+AGG + chr3_2:34838888-34838907 None:intergenic 25.0%
!! CATTTTATCTCATTTCCTTT+CGG - chr3_2:34839129-34839148 Msa0404130:intron 25.0%
! AATACGTAAGCAATAATTTG+AGG - chr3_2:34839382-34839401 Msa0404130:intron 25.0%
!!! TATACTGATAGTGCTTTTAT+GGG - chr3_2:34839512-34839531 Msa0404130:intron 25.0%
! CCGTAATATGCAATTTATAT+TGG + chr3_2:34839829-34839848 None:intergenic 25.0%
!! GAAAATTGAATTTGGGTTAT+GGG + chr3_2:34839995-34840014 None:intergenic 25.0%
!! TGAAAATTGAATTTGGGTTA+TGG + chr3_2:34839996-34840015 None:intergenic 25.0%
!! AAATCTTTGTTTATGTTTCC+AGG + chr3_2:34840199-34840218 None:intergenic 25.0%
! CTTTGATATAAACTTCTGTA+AGG + chr3_2:34840396-34840415 None:intergenic 25.0%
! TATATCAAAGATCTTAAGCT+TGG - chr3_2:34840406-34840425 Msa0404130:CDS 25.0%
!! GATATTATGGTTTTTCCTAA+AGG - chr3_2:34840430-34840449 Msa0404130:CDS 25.0%
! TTCACAAATTAAGAAGCTTA+AGG - chr3_2:34840606-34840625 Msa0404130:five_prime_UTR 25.0%
CACTAAAAGGGTTTGAATTA+GGG + chr3_2:34836064-34836083 None:intergenic 30.0%
ACACTAAAAGGGTTTGAATT+AGG + chr3_2:34836065-34836084 None:intergenic 30.0%
GGGATGATGAATTTCAAATT+CGG + chr3_2:34836144-34836163 None:intergenic 30.0%
AAATTCATCATCCCCAAAAA+TGG - chr3_2:34836150-34836169 Msa0404130:CDS 30.0%
AATTCATCATCCCCAAAAAT+GGG - chr3_2:34836151-34836170 Msa0404130:CDS 30.0%
!!! GAAGGTATTTTTGTGATTCT+TGG + chr3_2:34836234-34836253 None:intergenic 30.0%
!! AAGGAATTTGCTTTTGTTGA+TGG - chr3_2:34836620-34836639 Msa0404130:intron 30.0%
!!! AGAGTTTTGCTAGCTTTAAA+TGG - chr3_2:34836817-34836836 Msa0404130:intron 30.0%
!!! GAGTTTTGCTAGCTTTAAAT+GGG - chr3_2:34836818-34836837 Msa0404130:intron 30.0%
CAAGGAAAGATTAATCTGAT+GGG + chr3_2:34836872-34836891 None:intergenic 30.0%
CATCAGATTAATCTTTCCTT+GGG - chr3_2:34836871-34836890 Msa0404130:intron 30.0%
ACATTAAACAATCTTACCCA+AGG + chr3_2:34836890-34836909 None:intergenic 30.0%
GGTAAGATTGTTTAATGTGT+TGG - chr3_2:34836892-34836911 Msa0404130:intron 30.0%
TATAGATTAGTAGTTTGGAG+CGG - chr3_2:34836982-34837001 Msa0404130:intron 30.0%
!!! ATCCGTTATCTTTGTTTTGA+AGG - chr3_2:34837046-34837065 Msa0404130:intron 30.0%
!!! ATCTTTGTTTTGAAGGAATC+AGG - chr3_2:34837053-34837072 Msa0404130:intron 30.0%
!!! TCTTTGTTTTGAAGGAATCA+GGG - chr3_2:34837054-34837073 Msa0404130:intron 30.0%
GAATCAAAATCAGTTTGGAA+GGG - chr3_2:34837112-34837131 Msa0404130:intron 30.0%
! TTTTTCCAATTCTCCAGTTT+GGG + chr3_2:34837148-34837167 None:intergenic 30.0%
!!! TTTTTTCCAATTCTCCAGTT+TGG + chr3_2:34837149-34837168 None:intergenic 30.0%
! GATTGGTTGAAACTTGAAAT+CGG - chr3_2:34837327-34837346 Msa0404130:CDS 30.0%
TTGTACTAACTCAAGAACTA+TGG - chr3_2:34837635-34837654 Msa0404130:CDS 30.0%
!! AACTATGGATTGACTTTTGT+CGG - chr3_2:34837650-34837669 Msa0404130:CDS 30.0%
GTGATAGAACTTCATTAACA+CGG + chr3_2:34837700-34837719 None:intergenic 30.0%
! GAATTTATCTGACTCTCTTT+TGG - chr3_2:34837912-34837931 Msa0404130:intron 30.0%
TGATTCTACATATGCATTTC+TGG - chr3_2:34837957-34837976 Msa0404130:intron 30.0%
TTGAAAACGAGTCATGTAAA+AGG - chr3_2:34837989-34838008 Msa0404130:intron 30.0%
AAGATGAAAAATGGAGAATG+AGG + chr3_2:34838047-34838066 None:intergenic 30.0%
GTGAAATATGACTAGAAGTA+TGG + chr3_2:34838145-34838164 None:intergenic 30.0%
AATTAATCTTCACAAAGCTG+AGG - chr3_2:34838306-34838325 Msa0404130:intron 30.0%
TACCATGCTAAGTTAGATAA+GGG + chr3_2:34838404-34838423 None:intergenic 30.0%
ATACCATGCTAAGTTAGATA+AGG + chr3_2:34838405-34838424 None:intergenic 30.0%
TGAAAACAATATGTTGGACA+AGG - chr3_2:34838448-34838467 Msa0404130:intron 30.0%
TAAAAAAATCTAACTCGCCT+TGG + chr3_2:34838552-34838571 None:intergenic 30.0%
TCTCAAATTATTAGTGGTGT+CGG + chr3_2:34838753-34838772 None:intergenic 30.0%
! ATAATTATTTGTGTCGGTGT+TGG - chr3_2:34838790-34838809 Msa0404130:intron 30.0%
TTATCATGTGTATCTTCTGT+TGG - chr3_2:34838893-34838912 Msa0404130:intron 30.0%
TTTAAAAATCTAACTCGCCT+TGG + chr3_2:34839053-34839072 None:intergenic 30.0%
AGTAAATATCATCATCCGAA+AGG + chr3_2:34839147-34839166 None:intergenic 30.0%
TACTTATATAAGTTGCCTGA+TGG - chr3_2:34839163-34839182 Msa0404130:intron 30.0%
AGTCAAATATATATGAGACG+TGG + chr3_2:34839215-34839234 None:intergenic 30.0%
TTATATGGTAAGGGTGATAA+TGG + chr3_2:34839329-34839348 None:intergenic 30.0%
TCATCAACACATGTGTTAAT+TGG + chr3_2:34839457-34839476 None:intergenic 30.0%
CACTATCAGTATAGATTCTT+AGG + chr3_2:34839505-34839524 None:intergenic 30.0%
!! CTATACTGATAGTGCTTTTA+TGG - chr3_2:34839511-34839530 Msa0404130:intron 30.0%
CAGAAATCGTAACTTAGTTA+TGG - chr3_2:34839574-34839593 Msa0404130:intron 30.0%
! GAGTTTTCACATTTCAATGA+TGG - chr3_2:34839617-34839636 Msa0404130:intron 30.0%
AGATTAATCTCAAACTATGC+TGG + chr3_2:34839680-34839699 None:intergenic 30.0%
AACACAAAATCATCAACTGT+TGG + chr3_2:34840163-34840182 None:intergenic 30.0%
TAATTCAATGACAGATGCTT+TGG + chr3_2:34840236-34840255 None:intergenic 30.0%
ATTGAATTACCAAGTCTCAA+TGG - chr3_2:34840247-34840266 Msa0404130:intron 30.0%
ATTAGTAATTCAGTTGCATC+GGG + chr3_2:34840337-34840356 None:intergenic 30.0%
GATTAGTAATTCAGTTGCAT+CGG + chr3_2:34840338-34840357 None:intergenic 30.0%
TGCAACTGAATTACTAATCA+TGG - chr3_2:34840339-34840358 Msa0404130:CDS 30.0%
GAATTACTAATCATGGTTCA+AGG - chr3_2:34840346-34840365 Msa0404130:CDS 30.0%
! TCTTAAGCTTGGTGATATTA+TGG - chr3_2:34840417-34840436 Msa0404130:CDS 30.0%
ACAAAATGCATTAGTCCTTT+AGG + chr3_2:34840448-34840467 None:intergenic 30.0%
!!! ATGCATTTTGTTGTTAACTC+TGG - chr3_2:34840457-34840476 Msa0404130:CDS 30.0%
TAATCAAGATACTGAAAGCT+AGG + chr3_2:34840526-34840545 None:intergenic 30.0%
TGAATTGGGTGACACTAAAA+GGG + chr3_2:34836076-34836095 None:intergenic 35.0%
TTGAATTGGGTGACACTAAA+AGG + chr3_2:34836077-34836096 None:intergenic 35.0%
!! TAGGAGGATTTCCCATTTTT+GGG + chr3_2:34836165-34836184 None:intergenic 35.0%
ATGTAATCGTCCATTTCACT+AGG + chr3_2:34836184-34836203 None:intergenic 35.0%
!! GTTGATCTTTCTGGTTCAAT+TGG + chr3_2:34836282-34836301 None:intergenic 35.0%
!!! TGTTTTGGATTAGTCTGAAG+CGG - chr3_2:34836398-34836417 Msa0404130:CDS 35.0%
TGTCAGAAATTGTTGGATGA+AGG - chr3_2:34836480-34836499 Msa0404130:CDS 35.0%
GTCAGAAATTGTTGGATGAA+GGG - chr3_2:34836481-34836500 Msa0404130:CDS 35.0%
TTGCGTTGTATGAACATGAT+AGG - chr3_2:34836523-34836542 Msa0404130:intron 35.0%
TGCGTTGTATGAACATGATA+GGG - chr3_2:34836524-34836543 Msa0404130:intron 35.0%
!! CAAGCATCGAAACATTTCAA+TGG - chr3_2:34836659-34836678 Msa0404130:intron 35.0%
CCAAGGAAAGATTAATCTGA+TGG + chr3_2:34836873-34836892 None:intergenic 35.0%
CCATCAGATTAATCTTTCCT+TGG - chr3_2:34836870-34836889 Msa0404130:intron 35.0%
! TTCAAATTGAAGGCGTAACA+CGG + chr3_2:34836943-34836962 None:intergenic 35.0%
GGAATCAAAATCAGTTTGGA+AGG - chr3_2:34837111-34837130 Msa0404130:intron 35.0%
! TTTTCCAATTCTCCAGTTTG+GGG + chr3_2:34837147-34837166 None:intergenic 35.0%
GGAGAATTGGAAAAAACGAA+TGG - chr3_2:34837153-34837172 Msa0404130:intron 35.0%
CAGATCATTAGCGATTGTTT+GGG + chr3_2:34837189-34837208 None:intergenic 35.0%
TCAGATCATTAGCGATTGTT+TGG + chr3_2:34837190-34837209 None:intergenic 35.0%
TTATACTGCAGTGCTTCTTA+AGG - chr3_2:34837223-34837242 Msa0404130:intron 35.0%
TATACTGCAGTGCTTCTTAA+GGG - chr3_2:34837224-34837243 Msa0404130:intron 35.0%
ATCGCAAAACTGAGTCTTTA+TGG + chr3_2:34837272-34837291 None:intergenic 35.0%
AGAATGGAAGTCATTTGATG+TGG - chr3_2:34837305-34837324 Msa0404130:CDS 35.0%
AACTTAAGTCACTTACCCAT+AGG - chr3_2:34837390-34837409 Msa0404130:intron 35.0%
GAACTTAATTCACTCACCAT+AGG - chr3_2:34837454-34837473 Msa0404130:intron 35.0%
! ATTTCTTGTTGTGTTACCAC+TGG + chr3_2:34837563-34837582 None:intergenic 35.0%
ATGGGAAAAGCAAATATCTG+GGG + chr3_2:34837853-34837872 None:intergenic 35.0%
CATGGGAAAAGCAAATATCT+GGG + chr3_2:34837854-34837873 None:intergenic 35.0%
TCATGGGAAAAGCAAATATC+TGG + chr3_2:34837855-34837874 None:intergenic 35.0%
!! GATATTTGCTTTTCCCATGA+AGG - chr3_2:34837855-34837874 Msa0404130:intron 35.0%
AATTTGTAGCTTAGAGCCAT+TGG - chr3_2:34838219-34838238 Msa0404130:intron 35.0%
GACAAAACTCTATGAACCAA+TGG + chr3_2:34838238-34838257 None:intergenic 35.0%
!!! TTTCTGAAGCGACTTGATTT+TGG + chr3_2:34838269-34838288 None:intergenic 35.0%
ATCCCTTATCTAACTTAGCA+TGG - chr3_2:34838399-34838418 Msa0404130:CDS 35.0%
TATGGAAGAAATGCAAGGAA+AGG - chr3_2:34838483-34838502 Msa0404130:intron 35.0%
ATGGAAGAAATGCAAGGAAA+GGG - chr3_2:34838484-34838503 Msa0404130:intron 35.0%
ATGCTATGTACTCGAATATG+TGG - chr3_2:34838621-34838640 Msa0404130:CDS 35.0%
TGCTATGTACTCGAATATGT+GGG - chr3_2:34838622-34838641 Msa0404130:CDS 35.0%
GATAAAATGAATTGCAACCC+TGG + chr3_2:34839118-34839137 None:intergenic 35.0%
TCTGTGGAGTTATATGGTAA+GGG + chr3_2:34839338-34839357 None:intergenic 35.0%
! CACATGTGTTGATGAATCAT+AGG - chr3_2:34839462-34839481 Msa0404130:intron 35.0%
! AGCGTAGACATGTTATTTGT+AGG + chr3_2:34839550-34839569 None:intergenic 35.0%
ATTGGCAGAAGAAGAAACAT+GGG + chr3_2:34840077-34840096 None:intergenic 35.0%
! GTTTGCTACACAGAAATCAT+TGG + chr3_2:34840095-34840114 None:intergenic 35.0%
!!! GTTGATGATTTTGTGTTCTC+TGG - chr3_2:34840166-34840185 Msa0404130:CDS 35.0%
AGAGTAGACATTGGTGTAAA+TGG - chr3_2:34840289-34840308 Msa0404130:intron 35.0%
TGAGCTACTATAAGTAGCAA+AGG + chr3_2:34840500-34840519 None:intergenic 35.0%
GTCTGTGCAATTATAGAAGT+AGG + chr3_2:34840571-34840590 None:intergenic 35.0%
!! AGGAGGATTTCCCATTTTTG+GGG + chr3_2:34836164-34836183 None:intergenic 40.0%
! CTAGGAGGATTTCCCATTTT+TGG + chr3_2:34836166-34836185 None:intergenic 40.0%
TAATCGTCCATTTCACTAGG+AGG + chr3_2:34836181-34836200 None:intergenic 40.0%
!!! GAGAAAGGTTTTGTTCACGA+AGG + chr3_2:34836252-34836271 None:intergenic 40.0%
TCAATTGGAGGAGAAGAGAA+AGG + chr3_2:34836267-34836286 None:intergenic 40.0%
!! GATCTTTCTGGTTCAATTGG+AGG + chr3_2:34836279-34836298 None:intergenic 40.0%
TGAAGGGCAGAATTTGGAAA+AGG - chr3_2:34836497-34836516 Msa0404130:CDS 40.0%
CCACTGACACTTCAAATTGA+AGG + chr3_2:34836953-34836972 None:intergenic 40.0%
! CCTTCAATTTGAAGTGTCAG+TGG - chr3_2:34836950-34836969 Msa0404130:intron 40.0%
TCAAAATCAGTTTGGAAGGG+AGG - chr3_2:34837115-34837134 Msa0404130:intron 40.0%
CAAAATCAGTTTGGAAGGGA+GGG - chr3_2:34837116-34837135 Msa0404130:intron 40.0%
AAATCCCCAAACTGGAGAAT+TGG - chr3_2:34837140-34837159 Msa0404130:intron 40.0%
ATCATTAGCGATTGTTTGGG+AGG + chr3_2:34837186-34837205 None:intergenic 40.0%
CGCTAATGATCTGAGTTAGT+CGG - chr3_2:34837196-34837215 Msa0404130:intron 40.0%
ATACTGCAGTGCTTCTTAAG+GGG - chr3_2:34837225-34837244 Msa0404130:intron 40.0%
TGCTTCTTAAGGGGATATCA+AGG - chr3_2:34837234-34837253 Msa0404130:intron 40.0%
GCTTCTTAAGGGGATATCAA+GGG - chr3_2:34837235-34837254 Msa0404130:intron 40.0%
GGAAGTCATTTGATGTGGAT+TGG - chr3_2:34837310-34837329 Msa0404130:CDS 40.0%
GGTCAACATTGACAACCAAA+AGG + chr3_2:34837832-34837851 None:intergenic 40.0%
!! TTTGCTTTTCCCATGAAGGT+TGG - chr3_2:34837859-34837878 Msa0404130:intron 40.0%
GAAGAAGAACCAACCTTCAT+GGG + chr3_2:34837871-34837890 None:intergenic 40.0%
AGAAGAAGAACCAACCTTCA+TGG + chr3_2:34837872-34837891 None:intergenic 40.0%
TCTTCTTCTACATGCACAGT+TGG - chr3_2:34837883-34837902 Msa0404130:intron 40.0%
CTTCTTCTACATGCACAGTT+GGG - chr3_2:34837884-34837903 Msa0404130:intron 40.0%
TTCTTCTACATGCACAGTTG+GGG - chr3_2:34837885-34837904 Msa0404130:intron 40.0%
AAAATGGAGAATGAGGTTGC+AGG + chr3_2:34838040-34838059 None:intergenic 40.0%
CAAGTCGCTTCAGAAAGATA+AGG - chr3_2:34838273-34838292 Msa0404130:intron 40.0%
TAATCTTCACAAAGCTGAGG+AGG - chr3_2:34838309-34838328 Msa0404130:intron 40.0%
TGTTGGACAAGGTGAACAAA+AGG - chr3_2:34838459-34838478 Msa0404130:intron 40.0%
ACAAGGTGAACAAAAGGCTA+TGG - chr3_2:34838465-34838484 Msa0404130:intron 40.0%
AAGGCTATGGAAGAAATGCA+AGG - chr3_2:34838478-34838497 Msa0404130:intron 40.0%
TATGTTTCTCGCCTGAACTT+GGG + chr3_2:34838514-34838533 None:intergenic 40.0%
GCTATGTACTCGAATATGTG+GGG - chr3_2:34838623-34838642 Msa0404130:CDS 40.0%
CTATGTACTCGAATATGTGG+GGG - chr3_2:34838624-34838643 Msa0404130:CDS 40.0%
TATGTACTCGAATATGTGGG+GGG - chr3_2:34838625-34838644 Msa0404130:CDS 40.0%
ACCCAGATGGGTAATATCAT+CGG + chr3_2:34838863-34838882 None:intergenic 40.0%
AACTATTAGGCAACCCAGAT+GGG + chr3_2:34838875-34838894 None:intergenic 40.0%
TAACTATTAGGCAACCCAGA+TGG + chr3_2:34838876-34838895 None:intergenic 40.0%
TCCAAGAAAAAGTAGCTCAG+TGG - chr3_2:34839262-34839281 Msa0404130:intron 40.0%
!!! TCCACTGAGCTACTTTTTCT+TGG + chr3_2:34839266-34839285 None:intergenic 40.0%
GTCTGTGGAGTTATATGGTA+AGG + chr3_2:34839339-34839358 None:intergenic 40.0%
CTACAGTCTGTGGAGTTATA+TGG + chr3_2:34839344-34839363 None:intergenic 40.0%
TGTGGATATTCTACAGTCTG+TGG + chr3_2:34839354-34839373 None:intergenic 40.0%
CTTACGTATTCTACAGTCTG+TGG + chr3_2:34839372-34839391 None:intergenic 40.0%
CATTGGCAGAAGAAGAAACA+TGG + chr3_2:34840078-34840097 None:intergenic 40.0%
!! TCAACTGTTGGTGATTTGCA+TGG + chr3_2:34840151-34840170 None:intergenic 40.0%
GTTCAAGGTAAAGTTACAGC+TGG - chr3_2:34840361-34840380 Msa0404130:CDS 40.0%
!! TTTGTTGTTAACTCTGGTGC+TGG - chr3_2:34840463-34840482 Msa0404130:exon 40.0%
AAGCTTAAGGCTCCGTTTAA+CGG - chr3_2:34840619-34840638 Msa0404130:five_prime_UTR 40.0%
GGGAAATCCTCCTAGTGAAA+TGG - chr3_2:34836171-34836190 Msa0404130:CDS 45.0%
! GTTGGAGGAGTTGATCTTTC+TGG + chr3_2:34836291-34836310 None:intergenic 45.0%
GTTGGATGAAGGGCAGAATT+TGG - chr3_2:34836491-34836510 Msa0404130:CDS 45.0%
!! ACATGATAGGGAAGCGTTGA+TGG - chr3_2:34836536-34836555 Msa0404130:intron 45.0%
!! AGGGAAGCGTTGATGGAATT+TGG - chr3_2:34836543-34836562 Msa0404130:intron 45.0%
!! GGGAAGCGTTGATGGAATTT+GGG - chr3_2:34836544-34836563 Msa0404130:intron 45.0%
!!! TCAGTCGCGTGTTTTGGAAT+TGG - chr3_2:34836596-34836615 Msa0404130:intron 45.0%
!!! CGCGTGTTTTGGAATTGGAA+AGG - chr3_2:34836601-34836620 Msa0404130:intron 45.0%
TAATCCCACTGTCCACTTGT+GGG + chr3_2:34836847-34836866 None:intergenic 45.0%
! AGATTAGTAGTTTGGAGCGG+AGG - chr3_2:34836985-34837004 Msa0404130:intron 45.0%
! GATTAGTAGTTTGGAGCGGA+GGG - chr3_2:34836986-34837005 Msa0404130:intron 45.0%
! AGGGAAGGGTTTTTGACGAA+AGG - chr3_2:34837005-34837024 Msa0404130:intron 45.0%
! GGGAAGGGTTTTTGACGAAA+GGG - chr3_2:34837006-34837025 Msa0404130:intron 45.0%
! AAGGGTTTTTGACGAAAGGG+AGG - chr3_2:34837009-34837028 Msa0404130:intron 45.0%
! AGGGTTTTTGACGAAAGGGA+GGG - chr3_2:34837010-34837029 Msa0404130:intron 45.0%
TCACTCACCATAGGTTAGAG+TGG - chr3_2:34837463-34837482 Msa0404130:intron 45.0%
GTTTCAGCCACTCTAACCTA+TGG + chr3_2:34837473-34837492 None:intergenic 45.0%
GTAAAAGGCTGCTGAGTATG+TGG - chr3_2:34838004-34838023 Msa0404130:intron 45.0%
TAACTTAGCATGGTATGCGC+AGG - chr3_2:34838409-34838428 Msa0404130:intron 45.0%
GTATGTTTCTCGCCTGAACT+TGG + chr3_2:34838515-34838534 None:intergenic 45.0%
TCTAACTCGCCTTGGAAGAA+GGG + chr3_2:34838544-34838563 None:intergenic 45.0%
ATCTAACTCGCCTTGGAAGA+AGG + chr3_2:34838545-34838564 None:intergenic 45.0%
TGCTTCATAGTCGGCAAGAA+AGG + chr3_2:34838686-34838705 None:intergenic 45.0%
! TTCTTGCCGACTATGAAGCA+CGG - chr3_2:34838686-34838705 Msa0404130:CDS 45.0%
ATCGGCAACACTATGAAGCA+CGG + chr3_2:34838845-34838864 None:intergenic 45.0%
TGCCGATGATATTACCCATC+TGG - chr3_2:34838858-34838877 Msa0404130:intron 45.0%
GCCGATGATATTACCCATCT+GGG - chr3_2:34838859-34838878 Msa0404130:intron 45.0%
!! TCCACTGGGCTACTTGTTTT+GGG + chr3_2:34838974-34838993 None:intergenic 45.0%
ATGCAGAATATGCGCATTCC+AGG - chr3_2:34839097-34839116 Msa0404130:intron 45.0%
TGCAGAATATGCGCATTCCA+GGG - chr3_2:34839098-34839117 Msa0404130:intron 45.0%
GAAAAAGTAGCTCAGTGGAG+AGG - chr3_2:34839267-34839286 Msa0404130:intron 45.0%
GGGTTATGGGAATGTGAGAA+TGG + chr3_2:34839982-34840001 None:intergenic 45.0%
AACAATCCAGACACTCCTTC+AGG - chr3_2:34840121-34840140 Msa0404130:CDS 45.0%
GATTTGCATGGATAGCCTGA+AGG + chr3_2:34840139-34840158 None:intergenic 45.0%
ACATCGAGTCCATTGAGACT+TGG + chr3_2:34840259-34840278 None:intergenic 45.0%
AAACCGAAACCTTGTGGCGT+TGG + chr3_2:34836309-34836328 None:intergenic 50.0%
GGATCGAAACCGAAACCTTG+TGG + chr3_2:34836315-34836334 None:intergenic 50.0%
GGAGAATCAGAGACTTGCGT+CGG - chr3_2:34836455-34836474 Msa0404130:CDS 50.0%
GTCGGCGTGTCAGAAATTGT+TGG - chr3_2:34836473-34836492 Msa0404130:CDS 50.0%
AGCTGATGAACGTGAACGAC+AGG - chr3_2:34836572-34836591 Msa0404130:intron 50.0%
AATCCCACTGTCCACTTGTG+GGG + chr3_2:34836846-34836865 None:intergenic 50.0%
CTAATCCCACTGTCCACTTG+TGG + chr3_2:34836848-34836867 None:intergenic 50.0%
! GGGTTTTTGACGAAAGGGAG+GGG - chr3_2:34837011-34837030 Msa0404130:intron 50.0%
! GGTTTTTGACGAAAGGGAGG+GGG - chr3_2:34837012-34837031 Msa0404130:intron 50.0%
TCTTCACAAAGCTGAGGAGG+AGG - chr3_2:34838312-34838331 Msa0404130:intron 50.0%
GAAAGGGATGACCCAAGTTC+AGG - chr3_2:34838500-34838519 Msa0404130:intron 50.0%
CCGTATCCGTGCTTCATAGT+CGG + chr3_2:34838695-34838714 None:intergenic 50.0%
CCGACTATGAAGCACGGATA+CGG - chr3_2:34838692-34838711 Msa0404130:CDS 50.0%
ATGAAGCACGGATACGGACA+TGG - chr3_2:34838698-34838717 Msa0404130:CDS 50.0%
TATGAAGCACGGACGCTCTT+CGG + chr3_2:34838834-34838853 None:intergenic 50.0%
TCCCAAAACAAGTAGCCCAG+TGG - chr3_2:34838970-34838989 Msa0404130:intron 50.0%
! CTCCACTGGGCTACTTGTTT+TGG + chr3_2:34838975-34838994 None:intergenic 50.0%
TCCTTTCCTTCTGCTCCACT+GGG + chr3_2:34838988-34839007 None:intergenic 50.0%
TTCCTTTCCTTCTGCTCCAC+TGG + chr3_2:34838989-34839008 None:intergenic 50.0%
TATGTTTCTCGCCGGAGTTG+GGG + chr3_2:34839015-34839034 None:intergenic 50.0%
GTATGTTTCTCGCCGGAGTT+GGG + chr3_2:34839016-34839035 None:intergenic 50.0%
TCTAACTCGCCTTGGCAGAT+CGG + chr3_2:34839045-34839064 None:intergenic 50.0%
GGCAGAAGAAGAAACATGGG+AGG + chr3_2:34840074-34840093 None:intergenic 50.0%
!! GTGTTCTCTGGCTTTGTACC+TGG - chr3_2:34840178-34840197 Msa0404130:CDS 50.0%
! CAATGGACTCGATGTCTCTG+CGG - chr3_2:34840264-34840283 Msa0404130:intron 50.0%
TCTGCGGCAAGAGTAGACAT+TGG - chr3_2:34840280-34840299 Msa0404130:intron 50.0%
GCATCGGGATGAGTATGCAT+CGG + chr3_2:34840322-34840341 None:intergenic 50.0%
TCAACTCCTCCAACGCCACA+AGG - chr3_2:34836297-34836316 Msa0404130:CDS 55.0%
CCTCCAACGCCACAAGGTTT+CGG - chr3_2:34836303-34836322 Msa0404130:CDS 55.0%
! ACAGGCTCAGTCGCGTGTTT+TGG - chr3_2:34836590-34836609 Msa0404130:intron 55.0%
TTAAATGGGCCCCCCACAAG+TGG - chr3_2:34836832-34836851 Msa0404130:intron 55.0%
ATCCCACTGTCCACTTGTGG+GGG + chr3_2:34836845-34836864 None:intergenic 55.0%
GCGTAACACGGTGTTCACAC+AGG + chr3_2:34836931-34836950 None:intergenic 55.0%
! AGTAGTTTGGAGCGGAGGGA+AGG - chr3_2:34836990-34837009 Msa0404130:intron 55.0%
! GTAGTTTGGAGCGGAGGGAA+GGG - chr3_2:34836991-34837010 Msa0404130:intron 55.0%
GGGAGGGTAAATCCCCAAAC+TGG - chr3_2:34837132-34837151 Msa0404130:intron 55.0%
GGCTGCTGAGTATGTGGAAC+TGG - chr3_2:34838010-34838029 Msa0404130:intron 55.0%
TACCAGCAGCCCTTCTTCCA+AGG - chr3_2:34838532-34838551 Msa0404130:intron 55.0%
TACTCGAATATGTGGGGGGC+AGG - chr3_2:34838629-34838648 Msa0404130:CDS 55.0%
ACTCGAATATGTGGGGGGCA+GGG - chr3_2:34838630-34838649 Msa0404130:CDS 55.0%
ACGGATACGGACATGGACAC+CGG - chr3_2:34838705-34838724 Msa0404130:CDS 55.0%
GTCAATGTCCGTGTCGTGTC+CGG + chr3_2:34838727-34838746 None:intergenic 55.0%
AAGTAGCCCAGTGGAGCAGA+AGG - chr3_2:34838979-34838998 Msa0404130:intron 55.0%
GAAAGGAACCACCCCAACTC+CGG - chr3_2:34839001-34839020 Msa0404130:intron 55.0%
GGTATGTTTCTCGCCGGAGT+TGG + chr3_2:34839017-34839036 None:intergenic 55.0%
GCTGCTGGTATGTTTCTCGC+CGG + chr3_2:34838522-34838541 None:intergenic 55.0%
!! GGATAGCCTGAAGGAGTGTC+TGG + chr3_2:34840130-34840149 None:intergenic 55.0%
AAGGCTCCGTTTAACGGCAC+TGG - chr3_2:34840625-34840644 Msa0404130:five_prime_UTR 55.0%
! CCGAAACCTTGTGGCGTTGG+AGG + chr3_2:34836306-34836325 None:intergenic 60.0%
TCCCACTGTCCACTTGTGGG+GGG + chr3_2:34836844-34836863 None:intergenic 60.0%
CATGGACACCGGACACGACA+CGG - chr3_2:34838716-34838735 Msa0404130:CDS 60.0%
GCCCAGTGGAGCAGAAGGAA+AGG - chr3_2:34838984-34839003 Msa0404130:intron 60.0%
TACCAGCAGCCGATCTGCCA+AGG - chr3_2:34839033-34839052 Msa0404130:intron 60.0%
GCCCCCCACAAGTGGACAGT+GGG - chr3_2:34836840-34836859 Msa0404130:intron 65.0%
CGCCTTGGAAGAAGGGCTGC+TGG + chr3_2:34838537-34838556 None:intergenic 65.0%
GTTTCTCGCCGGAGTTGGGG+TGG + chr3_2:34839012-34839031 None:intergenic 65.0%
GGCCCCCCACAAGTGGACAG+TGG - chr3_2:34836839-34836858 Msa0404130:intron 70.0%
CGCCTTGGCAGATCGGCTGC+TGG + chr3_2:34839038-34839057 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 34835996 34840651 34835996 ID=Msa0404130;Name=Msa0404130
chr3_2 mRNA 34835996 34840651 34835996 ID=Msa0404130-mRNA-1;Parent=Msa0404130;Name=Msa0404130-mRNA-1;_AED=0.05;_eAED=0.05;_QI=173|0.28|0.25|1|1|1|8|0|531
chr3_2 exon 34835996 34836537 34835996 ID=Msa0404130-mRNA-1:exon:7125;Parent=Msa0404130-mRNA-1
chr3_2 exon 34837261 34837405 34837261 ID=Msa0404130-mRNA-1:exon:7124;Parent=Msa0404130-mRNA-1
chr3_2 exon 34837593 34837697 34837593 ID=Msa0404130-mRNA-1:exon:7123;Parent=Msa0404130-mRNA-1
chr3_2 exon 34838094 34838216 34838094 ID=Msa0404130-mRNA-1:exon:7122;Parent=Msa0404130-mRNA-1
chr3_2 exon 34838314 34838419 34838314 ID=Msa0404130-mRNA-1:exon:7121;Parent=Msa0404130-mRNA-1
chr3_2 exon 34838616 34838770 34838616 ID=Msa0404130-mRNA-1:exon:7120;Parent=Msa0404130-mRNA-1
chr3_2 exon 34839967 34840236 34839967 ID=Msa0404130-mRNA-1:exon:7119;Parent=Msa0404130-mRNA-1
chr3_2 exon 34840329 34840651 34840329 ID=Msa0404130-mRNA-1:exon:7118;Parent=Msa0404130-mRNA-1
chr3_2 five_prime_UTR 34840479 34840651 34840479 ID=Msa0404130-mRNA-1:five_prime_utr;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34840329 34840478 34840329 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34839967 34840236 34839967 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34838616 34838770 34838616 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34838314 34838419 34838314 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34838094 34838216 34838094 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34837593 34837697 34837593 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34837261 34837405 34837261 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
chr3_2 CDS 34835996 34836537 34835996 ID=Msa0404130-mRNA-1:cds;Parent=Msa0404130-mRNA-1
Gene Sequence

>Msa0404130

ATGGGAAATCCTCCTAGTGAAATGGACGATTACATCTTCAAACAACGCAAGCTTCATGAATCCCAAGAATCACAAAAATACCTTCGTGAACAAAACCTTTCTCTTCTCCTCCAATTGAACCAGAAAGATCAACTCCTCCAACGCCACAAGTCTGAAGCGGATATGAATGCACGAGCATTGAAGAAATTTGATTTGGAGAATCAGAGACTTGCGTCGGCGTGTCAGAAATTGTTGGATGAAGGGCAGAATTTGGAAAAGGAAGTTGCGTTGTATGAACATGATAGGGAAGCGTTGATGGAATTTGGGAACGAAGCTGATGAACGTGAACGACAGGCTCAGTCGCGTGTTTTGGAATTGGAAAGGAATTTGCTTTTGTTGATGGATGAATTGAAAAAATACAAGCATCGAAACATTTCAATGGTTGGTTCTTCTTCTACATGCACAGTTGGGGAAAAGAATTTATCTGACTCTCTTTTGGCAACACTTACTAACAAAGATGATGATTCTACATATGCATTTCTGGAATCAAATATTGAAAACGAGTCATGTAAAAGGCTGCTGAGTATGTGGAACTGCTTAGAGCCATTGGTTCATAGAGTTTTGTCTCTAGTTGCCAAAATCAAGTCGCTTCAGAAAGATAAGGAGCATCTAAGAATTAATCTTCACAAAGCTGAGGAGGAGGTCAAATTACTGTTTGATGAAAACAATATGTTGGACAAGGTGAACAAAAGGCTATGGAAGAAATGCAAGGAAAGGGATGACCCAAGTTCAGGCGAGAAACATACCAGCAGCCCTTCTTCCAAGTCAAACAAGAGAAAGTCTAGTCCCAAAACAAGTAGCCCAGTGGAGCAGAAGGAAAGGAACCACCCCAACTCCGGCGAGAAACATACCAGCAGCCGATCTGCCAAGTCAAACAAGAGAACGTCTAGTCCAAGAAAAAGTAGCTCAGTGGAGAGGAAGATTGATTTTGACGATCAAGATTCAGCAAGACAACCATTATCACCCTTACCATATAACTCCACAGACTGTAGAATATCCACAGACTGTAGAATACCAAACTTGCTCAACAATCCAGACACTCCTTCAGGCTATCCATGCAAATCACCAACAGTTGATGATTTTGTGTTCTCTGGCTTTGTACCTGGAAACATAAACAAAGATTTTAATGTAAAATTAACCAAAGCATCTGTCATTGAATTACCAAGTCTCAATGGACTCGATGTCTCTGCGGCAAGAGTAGACATTGGTGTAAATGGATCTGTTCCGATGCATACTCATCCCGATGCAACTGAATTACTAATCATGGTTCAAGGTAAAGTTACAGCTGGTTTTATTAACCTTACAGAAGTTTATATCAAAGATCTTAAGCTTGGTGATATTATGGTTTTTCCTAAAGGACTAATGCATTTTGTTGTTAACTCTGGTGCTGGAGAAGCTATTGCCTTTGCTACTTATAGTAGCTCAAAGCCTAGCTTTCAGTATCTTGATTATGTATTATTTAGAAACAACTTACCTACTTCTATAATTGCACAGACTACTCTTCTTGATGTTTCACAAATTAAGAAGCTTAAGGCTCCGTTTAACGGCACTGGCTAG
Protein sequence

>Msa0404130

MGNPPSEMDDYIFKQRKLHESQESQKYLREQNLSLLLQLNQKDQLLQRHKSEADMNARALKKFDLENQRLASACQKLLDEGQNLEKEVALYEHDREALMEFGNEADERERQAQSRVLELERNLLLLMDELKKYKHRNISMVGSSSTCTVGEKNLSDSLLATLTNKDDDSTYAFLESNIENESCKRLLSMWNCLEPLVHRVLSLVAKIKSLQKDKEHLRINLHKAEEEVKLLFDENNMLDKVNKRLWKKCKERDDPSSGEKHTSSPSSKSNKRKSSPKTSSPVEQKERNHPNSGEKHTSSRSAKSNKRTSSPRKSSSVERKIDFDDQDSARQPLSPLPYNSTDCRISTDCRIPNLLNNPDTPSGYPCKSPTVDDFVFSGFVPGNINKDFNVKLTKASVIELPSLNGLDVSAARVDIGVNGSVPMHTHPDATELLIMVQGKVTAGFINLTEVYIKDLKLGDIMVFPKGLMHFVVNSGAGEAIAFATYSSSKPSFQYLDYVLFRNNLPTSIIAQTTLLDVSQIKKLKAPFNGTG*