AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0464150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464150 MtrunA17_Chr3g0114711 86.000 400 19 2 1 366 1 397 0.0 686
Msa0464150 MtrunA17_Chr3g0114731 63.818 351 121 4 15 365 16 360 1.52e-162 461
Msa0464150 MtrunA17_Chr3g0114691 49.584 361 173 3 5 363 8 361 6.21e-129 375
Msa0464150 MtrunA17_Chr1g0160171 51.253 359 161 4 13 366 12 361 6.95e-126 367
Msa0464150 MtrunA17_Chr1g0160181 52.890 346 159 3 19 362 20 363 3.68e-124 363
Msa0464150 MtrunA17_Chr3g0114701 51.913 366 157 5 10 363 2 360 1.14e-123 361
Msa0464150 MtrunA17_Chr1g0182291 47.801 341 173 3 11 350 9 345 3.31e-118 347
Msa0464150 MtrunA17_Chr7g0263101 46.491 342 174 3 18 355 14 350 3.45e-115 339
Msa0464150 MtrunA17_Chr7g0255561 45.245 347 169 4 1 337 1 336 1.68e-111 330
Msa0464150 MtrunA17_Chr7g0255581 45.245 347 169 4 1 337 1 336 1.68e-111 330
Msa0464150 MtrunA17_Chr8g0354121 43.605 344 184 4 11 346 7 348 4.70e-105 314
Msa0464150 MtrunA17_Chr3g0081421 43.429 350 189 4 13 356 13 359 1.30e-104 313
Msa0464150 MtrunA17_Chr3g0081511 45.000 340 178 4 14 346 14 351 3.78e-103 309
Msa0464150 MtrunA17_Chr1g0177221 51.220 246 118 2 19 264 20 263 6.33e-84 256
Msa0464150 MtrunA17_Chr1g0186601 39.394 330 193 3 14 341 13 337 3.14e-82 254
Msa0464150 MtrunA17_Chr1g0186641 38.906 329 198 2 13 341 9 334 4.72e-82 254
Msa0464150 MtrunA17_Chr1g0171301 38.110 328 202 1 14 341 13 339 1.65e-79 248
Msa0464150 MtrunA17_Chr1g0186611 37.463 339 209 2 18 356 17 352 1.46e-78 245
Msa0464150 MtrunA17_Chr3g0142131 40.361 332 175 4 23 337 26 351 5.47e-77 242
Msa0464150 MtrunA17_Chr1g0186571 37.764 331 199 3 13 341 9 334 1.15e-76 240
Msa0464150 MtrunA17_Chr1g0186561 38.838 327 192 3 18 341 17 338 4.10e-76 239
Msa0464150 MtrunA17_Chr1g0155761 37.941 340 185 4 19 337 23 357 2.79e-75 238
Msa0464150 MtrunA17_Chr1g0184221 34.946 372 210 5 14 365 13 372 3.64e-73 232
Msa0464150 MtrunA17_Chr3g0129571 34.593 344 209 5 13 346 3 340 2.44e-72 229
Msa0464150 MtrunA17_Chr6g0483401 35.072 345 218 3 26 366 19 361 1.67e-70 224
Msa0464150 MtrunA17_Chr3g0129601 34.821 336 210 3 13 343 25 356 3.75e-70 224
Msa0464150 MtrunA17_Chr3g0129591 35.650 331 205 4 13 337 1 329 1.06e-67 217
Msa0464150 MtrunA17_Chr4g0050881 36.111 360 216 6 10 363 2 353 1.17e-67 217
Msa0464150 MtrunA17_Chr6g0463971 32.249 338 223 3 3 337 2 336 1.21e-66 214
Msa0464150 MtrunA17_Chr2g0284261 35.652 345 212 3 18 359 9 346 1.96e-65 211
Msa0464150 MtrunA17_Chr8g0383731 34.770 348 213 4 13 354 1 340 3.56e-65 210
Msa0464150 MtrunA17_Chr1g0164831 33.642 324 204 4 18 337 4 320 4.82e-62 202
Msa0464150 MtrunA17_Chr2g0278561 32.143 364 232 5 7 358 5 365 4.17e-60 198
Msa0464150 MtrunA17_Chr3g0129581 35.241 332 205 6 13 337 1 329 1.98e-59 196
Msa0464150 MtrunA17_Chr7g0271561 31.118 331 219 2 18 341 8 336 3.71e-59 196
Msa0464150 MtrunA17_Chr8g0334761 34.085 355 215 5 18 363 11 355 3.68e-58 192
Msa0464150 MtrunA17_Chr2g0290611 34.030 335 215 3 13 344 8 339 1.47e-57 191
Msa0464150 MtrunA17_Chr3g0126101 34.421 337 203 5 34 366 23 345 1.54e-57 190
Msa0464150 MtrunA17_Chr4g0073241 32.295 353 219 9 18 361 9 350 1.43e-56 189
Msa0464150 MtrunA17_Chr2g0278551 33.523 352 224 5 18 366 13 357 3.77e-56 188
Msa0464150 MtrunA17_Chr2g0278541 32.000 350 216 6 11 346 6 347 2.48e-55 186
Msa0464150 MtrunA17_Chr5g0413921 33.841 328 210 6 23 347 13 336 5.45e-54 181
Msa0464150 MtrunA17_Chr8g0334771 34.012 344 218 3 18 357 11 349 9.90e-54 181
Msa0464150 MtrunA17_Chr5g0413941 33.945 327 200 6 23 342 13 330 1.94e-53 180
Msa0464150 MtrunA17_Chr2g0332001 31.288 326 204 3 21 337 42 356 3.22e-53 181
Msa0464150 MtrunA17_Chr4g0059311 32.770 296 195 3 43 337 38 330 4.84e-53 179
Msa0464150 MtrunA17_Chr5g0413961 34.545 330 204 4 11 337 8 328 2.13e-52 177
Msa0464150 MtrunA17_Chr2g0310441 33.230 322 210 3 18 337 11 329 2.32e-52 177
Msa0464150 MtrunA17_Chr4g0050891 32.110 327 215 3 11 332 7 331 2.29e-50 172
Msa0464150 MtrunA17_Chr1g0160111 28.775 351 225 6 26 358 16 359 3.06e-50 172
Msa0464150 MtrunA17_Chr5g0413951 33.030 330 211 6 13 337 1 325 3.60e-50 171
Msa0464150 MtrunA17_Chr1g0150151 30.678 339 221 6 18 346 12 346 5.28e-48 166
Msa0464150 MtrunA17_Chr5g0413931 30.857 350 232 6 13 357 2 346 4.88e-47 163
Msa0464150 MtrunA17_Chr8g0334751 31.389 360 237 6 10 365 4 357 3.74e-46 161
Msa0464150 MtrunA17_Chr5g0408001 31.790 324 201 5 30 337 23 342 5.86e-46 161
Msa0464150 MtrunA17_Chr7g0227921 34.551 301 182 5 43 337 37 328 1.07e-45 160
Msa0464150 MtrunA17_Chr5g0407971 31.231 333 209 5 30 346 24 352 1.14e-44 158
Msa0464150 MtrunA17_Chr4g0051021 28.746 327 229 2 13 337 10 334 1.39e-44 157
Msa0464150 MtrunA17_Chr5g0441761 29.360 344 222 6 25 365 22 347 1.19e-43 154
Msa0464150 MtrunA17_Chr4g0050941 31.304 345 217 7 11 342 6 343 5.28e-43 153
Msa0464150 MtrunA17_Chr5g0408031 31.627 332 207 6 23 337 16 344 2.52e-42 151
Msa0464150 MtrunA17_Chr5g0428881 27.988 343 230 5 31 366 32 364 1.07e-41 150
Msa0464150 MtrunA17_Chr5g0407991 30.086 349 212 6 10 337 4 341 1.76e-41 149
Msa0464150 MtrunA17_Chr5g0408041 30.606 330 209 5 25 337 19 345 3.15e-41 149
Msa0464150 MtrunA17_Chr8g0369841 29.254 335 216 6 29 346 20 350 4.60e-39 143
Msa0464150 MtrunA17_Chr6g0451201 32.278 316 206 5 34 349 30 337 6.70e-39 142
Msa0464150 MtrunA17_Chr6g0451211 31.546 317 205 6 38 349 33 342 2.20e-38 140
Msa0464150 MtrunA17_Chr1g0211801 31.429 245 151 4 11 247 5 240 1.35e-37 136
Msa0464150 MtrunA17_Chr3g0126091 26.611 357 243 5 18 366 12 357 4.14e-37 137
Msa0464150 MtrunA17_Chr3g0084091 29.845 258 163 4 97 349 10 254 1.07e-36 134
Msa0464150 MtrunA17_Chr4g0050931 29.042 334 189 5 11 337 6 298 1.58e-36 134
Msa0464150 MtrunA17_Chr6g0451261 29.043 303 209 5 18 318 12 310 1.59e-34 134
Msa0464150 MtrunA17_Chr4g0051001 28.155 309 201 4 18 315 32 330 6.67e-34 128
Msa0464150 MtrunA17_Chr6g0451171 29.377 337 223 7 18 349 13 339 8.51e-34 128
Msa0464150 MtrunA17_Chr6g0451251 32.573 307 196 6 34 337 30 328 6.55e-33 126
Msa0464150 MtrunA17_Chr5g0404811 31.718 227 142 4 130 348 6 227 1.26e-32 122
Msa0464150 MtrunA17_Chr1g0182261 41.549 142 80 3 204 343 76 216 1.12e-30 116
Msa0464150 MtrunA17_Chr4g0056081 28.571 329 222 8 23 343 20 343 3.02e-30 118
Msa0464150 MtrunA17_Chr5g0429001 25.874 286 191 4 97 365 31 312 2.14e-29 115
Msa0464150 MtrunA17_Chr2g0278021 31.250 224 145 4 131 347 1 222 1.04e-28 112
Msa0464150 MtrunA17_Chr6g0451191 30.357 224 141 3 131 349 1 214 5.56e-28 109
Msa0464150 MtrunA17_Chr5g0399281 27.829 327 220 7 23 341 14 332 8.70e-28 111
Msa0464150 MtrunA17_Chr1g0172881 40.146 137 69 3 130 264 23 148 6.89e-27 104
Msa0464150 MtrunA17_Chr1g0177231 47.748 111 42 3 257 361 17 117 1.27e-24 97.4
Msa0464150 MtrunA17_Chr8g0334711 31.122 196 129 2 18 210 11 203 5.77e-24 98.2
Msa0464150 MtrunA17_Chr5g0404801 41.026 117 69 0 14 130 4 120 1.79e-22 91.7
Msa0464150 MtrunA17_Chr1g0172891 45.631 103 41 3 264 361 3 95 1.65e-20 85.9
Msa0464150 MtrunA17_Chr8g0334721 31.852 135 90 1 227 361 1 133 1.21e-18 82.4
Msa0464150 MtrunA17_Chr8g0334731 35.897 117 73 1 96 210 71 187 1.24e-15 74.7
Msa0464150 MtrunA17_Chr2g0278031 34.545 110 72 0 18 127 15 124 2.21e-13 66.6
Msa0464150 MtrunA17_Chr6g0451241 43.939 66 37 0 284 349 26 91 5.43e-13 65.1
Msa0464150 MtrunA17_Chr4g0050911 35.714 84 54 0 102 185 16 99 8.53e-13 65.9
Msa0464150 MtrunA17_Chr4g0050921 34.615 104 66 1 215 318 3 104 9.79e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464150 AT4G08290.1 57.627 354 139 5 13 363 8 353 6.78e-147 421
Msa0464150 AT4G08300.1 50.000 362 162 4 10 365 3 351 5.75e-125 364
Msa0464150 AT1G44800.1 50.140 357 164 5 10 363 3 348 1.97e-124 363
Msa0464150 AT1G21890.1 49.724 362 165 4 13 359 6 365 4.61e-122 358
Msa0464150 AT2G37460.1 46.686 347 180 3 11 356 8 350 7.33e-116 342
Msa0464150 AT1G21890.2 50.000 312 145 2 13 315 6 315 9.07e-108 320
Msa0464150 AT2G39510.1 44.000 350 184 4 18 366 8 346 7.28e-107 318
Msa0464150 AT4G08290.2 59.766 256 97 4 13 265 8 260 1.15e-106 315
Msa0464150 AT5G07050.1 43.768 345 182 4 1 337 3 343 3.84e-102 307
Msa0464150 AT2G40900.1 43.478 345 187 3 10 353 2 339 8.63e-101 303
Msa0464150 AT2G37450.2 44.380 347 167 4 13 358 4 325 1.74e-93 283
Msa0464150 AT3G56620.1 43.252 326 178 3 10 334 2 321 5.20e-92 280
Msa0464150 AT5G13670.1 38.393 336 202 4 12 344 2 335 1.44e-82 256
Msa0464150 AT2G37450.3 47.471 257 130 3 103 358 1 253 4.38e-76 236
Msa0464150 AT1G75500.1 38.037 326 193 3 19 337 21 344 4.25e-74 235
Msa0464150 AT1G75500.2 38.037 326 193 3 19 337 21 344 4.25e-74 235
Msa0464150 AT3G18200.2 34.783 368 227 7 2 365 22 380 3.13e-71 227
Msa0464150 AT5G64700.1 34.535 333 209 2 12 337 2 332 6.27e-66 213
Msa0464150 AT3G53210.1 37.195 328 193 4 14 335 6 326 6.88e-65 211
Msa0464150 AT3G30340.1 35.161 310 196 4 40 347 33 339 9.79e-63 205
Msa0464150 AT2G37450.1 45.217 230 121 3 130 358 61 286 1.67e-61 199
Msa0464150 AT4G30420.2 34.043 329 212 2 12 337 3 329 2.62e-58 194
Msa0464150 AT1G09380.1 32.308 325 206 4 18 334 8 326 7.31e-58 192
Msa0464150 AT4G30420.1 34.810 316 201 2 25 337 6 319 2.25e-57 191
Msa0464150 AT1G43650.1 34.921 315 200 2 23 337 12 321 2.71e-56 187
Msa0464150 AT1G25270.1 34.104 346 212 7 23 361 8 344 3.17e-54 182
Msa0464150 AT1G68170.1 34.286 350 222 3 23 366 8 355 1.74e-52 178
Msa0464150 AT1G68170.2 34.286 350 222 3 23 366 18 365 2.56e-52 178
Msa0464150 AT4G28040.1 31.953 338 211 5 12 345 2 324 3.04e-51 175
Msa0464150 AT4G28040.5 31.953 338 211 5 12 345 2 324 3.04e-51 175
Msa0464150 AT4G28040.3 31.953 338 211 5 12 345 2 324 3.04e-51 175
Msa0464150 AT4G28040.4 31.953 338 211 5 12 345 2 324 3.04e-51 175
Msa0464150 AT4G28040.2 31.953 338 211 5 12 345 2 324 3.04e-51 175
Msa0464150 AT1G01070.1 32.335 334 218 4 13 342 11 340 1.70e-50 173
Msa0464150 AT3G53210.2 39.516 248 137 4 94 335 29 269 2.83e-48 166
Msa0464150 AT5G40230.1 32.081 346 212 6 18 348 21 358 4.21e-48 167
Msa0464150 AT4G01440.1 27.457 346 236 2 15 360 7 337 5.54e-48 166
Msa0464150 AT3G53210.3 37.209 258 149 4 14 265 6 256 8.84e-48 163
Msa0464150 AT1G11450.3 31.250 336 223 4 13 345 11 341 4.32e-47 164
Msa0464150 AT4G01430.1 33.115 305 194 7 39 337 30 330 9.20e-46 160
Msa0464150 AT5G40240.1 31.003 329 220 4 18 342 20 345 5.68e-45 159
Msa0464150 AT5G40240.3 31.003 329 220 4 18 342 20 345 5.68e-45 159
Msa0464150 AT5G40240.2 31.003 329 220 4 18 342 34 359 1.01e-44 158
Msa0464150 AT3G28130.2 31.250 304 201 6 38 337 33 332 2.74e-44 156
Msa0464150 AT1G11450.5 32.997 297 191 4 52 345 6 297 5.35e-44 154
Msa0464150 AT3G28130.5 33.333 240 156 3 100 337 9 246 2.40e-43 151
Msa0464150 AT3G28130.1 33.333 240 156 3 100 337 9 246 2.40e-43 151
Msa0464150 AT1G11460.1 31.818 330 212 6 14 337 12 334 2.58e-43 153
Msa0464150 AT3G18200.1 32.812 256 162 6 114 365 1 250 6.60e-43 150
Msa0464150 AT1G01070.2 33.099 284 182 4 63 342 14 293 7.08e-43 152
Msa0464150 AT1G11450.2 32.765 293 189 4 56 345 3 290 8.60e-43 151
Msa0464150 AT3G28080.5 33.473 239 155 3 101 337 8 244 1.11e-42 150
Msa0464150 AT3G28080.7 33.473 239 155 3 101 337 8 244 1.11e-42 150
Msa0464150 AT3G28080.8 33.473 239 155 3 101 337 8 244 1.11e-42 150
Msa0464150 AT3G28080.6 33.473 239 155 3 101 337 8 244 1.11e-42 150
Msa0464150 AT3G28080.1 32.836 268 171 5 74 337 71 333 1.33e-42 152
Msa0464150 AT4G01440.3 27.907 301 215 1 15 315 7 305 2.78e-42 150
Msa0464150 AT3G28070.2 31.111 270 168 5 100 359 7 268 3.58e-42 148
Msa0464150 AT5G64700.2 31.923 260 168 2 12 264 2 259 6.10e-42 147
Msa0464150 AT3G28080.2 33.473 239 155 3 101 337 89 325 7.47e-42 150
Msa0464150 AT3G28100.1 30.408 319 214 6 23 337 19 333 1.78e-41 149
Msa0464150 AT3G28070.1 31.061 264 176 4 100 359 99 360 3.06e-41 148
Msa0464150 AT3G45870.1 29.538 325 215 3 19 332 13 334 8.30e-41 148
Msa0464150 AT1G70260.1 28.402 338 224 4 12 337 6 337 1.22e-40 147
Msa0464150 AT3G28050.1 32.353 306 197 6 34 337 29 326 2.22e-40 146
Msa0464150 AT5G40210.1 28.869 336 214 10 10 341 4 318 5.92e-40 144
Msa0464150 AT5G45370.1 28.662 314 208 4 31 342 33 332 2.12e-39 143
Msa0464150 AT3G30340.2 32.365 241 157 3 40 279 33 268 2.40e-39 141
Msa0464150 AT4G15540.1 28.655 342 230 4 18 358 17 345 6.55e-39 142
Msa0464150 AT4G15540.2 28.655 342 230 4 18 358 17 345 6.55e-39 142
Msa0464150 AT4G01430.2 32.862 283 180 7 39 315 30 308 2.48e-38 139
Msa0464150 AT5G45370.2 28.012 332 211 5 31 342 33 356 2.49e-38 141
Msa0464150 AT5G45370.4 28.012 332 211 5 31 342 33 356 2.49e-38 141
Msa0464150 AT4G19185.1 28.324 346 222 4 30 356 33 371 5.85e-38 140
Msa0464150 AT1G11450.4 30.392 306 205 4 13 315 11 311 1.46e-37 137
Msa0464150 AT4G01450.2 30.872 298 200 3 40 337 32 323 5.53e-37 137
Msa0464150 AT4G01450.1 32.906 234 151 3 40 273 32 259 8.69e-37 136
Msa0464150 AT4G01450.4 35.377 212 131 3 40 251 32 237 1.06e-36 134
Msa0464150 AT1G11450.1 32.806 253 162 4 96 345 2 249 1.07e-36 134
Msa0464150 AT5G47470.1 28.483 323 218 4 23 341 35 348 1.12e-35 134
Msa0464150 AT5G47470.2 28.483 323 218 4 23 341 63 376 1.76e-35 134
Msa0464150 AT5G47470.3 28.483 323 218 4 23 341 63 376 2.08e-35 133
Msa0464150 AT4G01440.4 29.870 231 162 0 15 245 7 237 2.41e-35 130
Msa0464150 AT3G45870.8 27.922 308 208 3 19 315 13 317 1.22e-33 127
Msa0464150 AT1G60050.1 27.401 354 245 5 18 361 13 364 1.60e-33 128
Msa0464150 AT4G01450.3 35.176 199 125 2 40 238 32 226 1.86e-32 122
Msa0464150 AT3G28070.3 32.110 218 144 3 100 315 99 314 4.05e-32 123
Msa0464150 AT4G24980.1 29.052 327 160 7 39 364 8 263 4.73e-32 122
Msa0464150 AT1G43650.2 32.677 254 165 3 23 275 12 260 5.98e-31 119
Msa0464150 AT3G28130.3 30.493 223 145 4 131 345 1 221 2.24e-30 116
Msa0464150 AT3G45870.9 30.126 239 156 1 19 246 13 251 2.30e-30 117
Msa0464150 AT3G28080.9 30.622 209 141 3 131 337 1 207 8.59e-30 114
Msa0464150 AT3G28070.5 28.870 239 152 5 131 359 1 231 3.35e-29 113
Msa0464150 AT4G01440.5 25.620 242 165 2 119 360 3 229 3.81e-29 114
Msa0464150 AT4G01440.2 25.620 242 165 2 119 360 3 229 3.81e-29 114
Msa0464150 AT4G19185.2 27.046 281 179 4 95 356 20 293 1.76e-27 110
Msa0464150 AT3G28130.4 30.973 226 149 5 38 259 33 255 1.52e-26 107
Msa0464150 AT3G28080.3 29.851 268 125 7 74 337 71 279 4.13e-25 103
Msa0464150 AT3G28080.4 30.126 239 109 5 101 337 8 190 5.35e-25 101
Msa0464150 AT4G16620.1 25.150 334 240 4 26 358 20 344 2.92e-24 102
Msa0464150 AT3G28100.3 29.876 241 162 5 23 259 19 256 3.24e-24 100
Msa0464150 AT5G45370.6 26.848 257 160 5 101 337 1 249 9.53e-24 99.8
Msa0464150 AT5G45370.5 26.848 257 160 5 101 337 1 249 9.53e-24 99.8
Msa0464150 AT3G45870.5 29.461 241 153 5 103 332 25 259 8.89e-21 92.0
Msa0464150 AT4G01430.3 29.442 197 130 6 146 337 1 193 2.45e-18 83.6
Msa0464150 AT3G28130.6 35.036 137 85 3 38 172 33 167 3.66e-18 82.8
Msa0464150 AT4G16620.4 26.953 256 178 3 103 358 1 247 1.11e-17 82.4
Msa0464150 AT4G16620.2 26.953 256 178 3 103 358 1 247 1.11e-17 82.4
Msa0464150 AT3G28100.2 30.729 192 127 4 23 210 19 208 2.41e-17 81.3
Msa0464150 AT5G45370.3 25.108 231 145 5 132 342 74 296 2.41e-15 76.6
Msa0464150 AT4G16620.3 27.014 211 151 3 26 235 20 228 2.50e-15 75.9
Msa0464150 AT3G45870.6 28.431 204 129 5 103 295 25 222 3.21e-14 72.4
Msa0464150 AT3G45870.7 28.431 204 129 5 103 295 25 222 3.21e-14 72.4
Msa0464150 AT3G45870.2 26.882 186 122 3 158 332 1 183 2.77e-12 66.2
Msa0464150 AT3G45870.3 30.323 155 94 3 103 246 25 176 4.83e-11 62.0

Find 89 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATAGGTGTTTCTAATTT+TGG 0.097188 3_3:+63777827 Msa0464150:CDS
GGTCTTGAGGTTTGGGCTAT+TGG 0.175402 3_3:+63781066 Msa0464150:CDS
TCAACCTTAATATGCTTAAT+TGG 0.206118 3_3:+63780997 Msa0464150:CDS
GATCAAGGATTTACTTTCTT+GGG 0.226580 3_3:+63780359 Msa0464150:CDS
ATGATCATCACTTGTGCTTT+AGG 0.227026 3_3:+63782022 Msa0464150:CDS
GTCACTCTGGTCTTGAGGTT+TGG 0.255637 3_3:+63781058 Msa0464150:CDS
GCACTTCAATTTGGGTTTGC+AGG 0.256620 3_3:+63777890 Msa0464150:CDS
TACAACTTTGCAGGAAAATT+AGG 0.260312 3_3:+63778450 Msa0464150:intron
AGCACCAATTAAGCATATTA+AGG 0.276307 3_3:-63781001 None:intergenic
GGTTGGGACTTTAGACTATA+TGG 0.302017 3_3:+63781087 Msa0464150:CDS
TTAACGGTGGCACTTCAATT+TGG 0.325006 3_3:+63777881 Msa0464150:CDS
GGGACATTAGTAACCTTTGC+AGG 0.325264 3_3:+63780660 Msa0464150:CDS
AAGACACCCATTGTCATCTT+TGG 0.325604 3_3:-63778473 None:intergenic
TCACTCTGGTCTTGAGGTTT+GGG 0.326320 3_3:+63781059 Msa0464150:CDS
TAGACATCAGATGAAGTAAT+TGG 0.329648 3_3:-63782433 None:intergenic
TTTGTTGATTACAGTATCAT+TGG 0.334370 3_3:+63782289 Msa0464150:intron
TTGAGGTTTGGGCTATTGGT+TGG 0.342298 3_3:+63781070 Msa0464150:CDS
AGATCAAGGATTTACTTTCT+TGG 0.355086 3_3:+63780358 Msa0464150:CDS
TCAGTTTGCTTCATTGCAAC+TGG 0.357064 3_3:-63782400 None:intergenic
ATAGACCCACTGCAATAATC+AGG 0.361308 3_3:-63782315 None:intergenic
TAACGGTGGCACTTCAATTT+GGG 0.361637 3_3:+63777882 Msa0464150:CDS
TGAGGTTTGGGCTATTGGTT+GGG 0.366132 3_3:+63781071 Msa0464150:CDS
AGTGGAGTTGCTCTGCAAAA+AGG 0.371654 3_3:-63782051 None:intergenic
ATTTGGTTACCTGAAAATTA+CGG 0.378910 3_3:-63780453 None:intergenic
TTAAATGCTGTATAAAATAC+TGG 0.379245 3_3:-63781989 None:intergenic
AGGAACACTCTTTCTAATGA+TGG 0.410004 3_3:+63780794 Msa0464150:CDS
ACTCTCAAACATTGGGTCTC+AGG 0.410947 3_3:+63780774 Msa0464150:CDS
GGTAATAAAATGAATAAGGC+AGG 0.427576 3_3:+63777848 Msa0464150:CDS
GGGTCTATATTCTGTGGTGT+GGG 0.434106 3_3:+63782330 Msa0464150:CDS
CGAAGCATAGCAAAAGTGAT+TGG 0.452310 3_3:+63780639 Msa0464150:CDS
TTTAGGCCAGTGATAGATCA+AGG 0.452691 3_3:+63780344 Msa0464150:intron
ACACGCAAACTCTCAAACAT+TGG 0.463592 3_3:+63780766 Msa0464150:CDS
GGGTGTCTTTGTGCGGATAA+TGG 0.463680 3_3:+63778487 Msa0464150:CDS
GTTGATAGTGACATTTCTGC+TGG 0.464929 3_3:-63780979 None:intergenic
TAATGATGGGTTGTGTGGCT+TGG 0.467472 3_3:+63780808 Msa0464150:CDS
GGCAGGGCCTTATGTGTTAA+CGG 0.467713 3_3:+63777865 Msa0464150:CDS
TGGGTCTATATTCTGTGGTG+TGG 0.473719 3_3:+63782329 Msa0464150:CDS
TGCTTCATTGCAACTGGTGA+TGG 0.475348 3_3:-63782394 None:intergenic
CACGCAAACTCTCAAACATT+GGG 0.482922 3_3:+63780767 Msa0464150:CDS
ATTCATTCCCATGTTGAGAC+TGG 0.492222 3_3:-63777933 None:intergenic
ATTAGGCCAAAGATGACAAT+GGG 0.506829 3_3:+63778467 Msa0464150:CDS
CTCCATGGCCAGTCTCAACA+TGG 0.517219 3_3:+63777925 Msa0464150:CDS
TTTCAGGGAATAATTACCTC+AGG 0.519170 3_3:+63781923 Msa0464150:intron
GGAACACTCTTTCTAATGAT+GGG 0.519597 3_3:+63780795 Msa0464150:CDS
TGGAGCCCTGATTATTGCAG+TGG 0.520137 3_3:+63782309 Msa0464150:CDS
GAAGCATAGCAAAAGTGATT+GGG 0.521865 3_3:+63780640 Msa0464150:CDS
GTAAATCCTTGATCTATCAC+TGG 0.527191 3_3:-63780350 None:intergenic
ACAAGTGATGATCATACAAA+GGG 0.529299 3_3:-63782014 None:intergenic
TCCCATGTTGAGACTGGCCA+TGG 0.532428 3_3:-63777927 None:intergenic
TTCATTGCTGAGCGTCACTC+TGG 0.533655 3_3:+63781045 Msa0464150:CDS
CTTTCTAATGATGGGTTGTG+TGG 0.538049 3_3:+63780803 Msa0464150:CDS
AGTGCCACCGTTAACACATA+AGG 0.539701 3_3:-63777872 None:intergenic
GAATAGAAAAGGTGAATTTG+TGG 0.542285 3_3:-63780711 None:intergenic
ATCAAGGATTTACTTTCTTG+GGG 0.542739 3_3:+63780360 Msa0464150:CDS
AATAGAAAAGGTGAATTTGT+GGG 0.544421 3_3:-63780710 None:intergenic
CTTGGGGATGCAATATACAT+CGG 0.549174 3_3:+63780376 Msa0464150:CDS
AGCGTACTATGTGCAAGGAT+TGG 0.550924 3_3:+63781949 Msa0464150:CDS
TGAGCGTCACTCTGGTCTTG+AGG 0.557312 3_3:+63781053 Msa0464150:CDS
CAGAGCAACTCCACTTGGGA+AGG 0.561936 3_3:+63782059 Msa0464150:CDS
GGACATTAGTAACCTTTGCA+GGG 0.563644 3_3:+63780661 Msa0464150:CDS
TTCATTGCAACTGGTGATGG+TGG 0.573404 3_3:-63782391 None:intergenic
GGAGCCCTGATTATTGCAGT+GGG 0.579920 3_3:+63782310 Msa0464150:CDS
GGTCTATATTCTGTGGTGTG+GGG 0.589276 3_3:+63782331 Msa0464150:CDS
GCAACTGGTGATGGTGGCAT+AGG 0.594061 3_3:-63782385 None:intergenic
CACAAGTGATGATCATACAA+AGG 0.594526 3_3:-63782015 None:intergenic
TTTGCAGAGCAACTCCACTT+GGG 0.594536 3_3:+63782055 Msa0464150:CDS
AATTAGGCCAAAGATGACAA+TGG 0.598592 3_3:+63778466 Msa0464150:CDS
CATCATCAATGCCCCTGCAA+AGG 0.600667 3_3:-63780673 None:intergenic
GTAATAAAATGAATAAGGCA+GGG 0.617635 3_3:+63777849 Msa0464150:CDS
GAAAATTACGGCTAGCACAA+AGG 0.618070 3_3:-63780441 None:intergenic
TTAGAAGTTACCTTCCCAAG+TGG 0.619720 3_3:-63782069 None:intergenic
TAGACCCACTGCAATAATCA+GGG 0.620855 3_3:-63782314 None:intergenic
GGAATAGCGTACTATGTGCA+AGG 0.623032 3_3:+63781944 Msa0464150:CDS
TGCAGTGGGTCTATATTCTG+TGG 0.631521 3_3:+63782324 Msa0464150:CDS
GGATTGGTATTTCAAAGCAG+AGG 0.631848 3_3:+63781965 Msa0464150:CDS
AGGAGCATACATATTCTCCA+TGG 0.632777 3_3:+63777910 Msa0464150:CDS
GCTAGCACAAAGGTAACAGA+AGG 0.636570 3_3:-63780431 None:intergenic
GACATTAGTAACCTTTGCAG+GGG 0.641806 3_3:+63780662 Msa0464150:CDS
ACTATATGGTCCTCTCTACA+CGG 0.654238 3_3:+63781101 Msa0464150:CDS
CACATAGTACGCTATTCCTG+AGG 0.655653 3_3:-63781939 None:intergenic
ATAGAGGGTACCGTGTAGAG+AGG 0.656220 3_3:-63781111 None:intergenic
TCCATGGCCAGTCTCAACAT+GGG 0.656868 3_3:+63777926 Msa0464150:CDS
TTGATGATGATACTTTACAA+AGG 0.659362 3_3:+63780687 Msa0464150:CDS
TGCTATGCAAACTAGTGTGG+TGG 0.665301 3_3:+63781020 Msa0464150:CDS
TGGTGCTATGCAAACTAGTG+TGG 0.682257 3_3:+63781017 Msa0464150:CDS
TGACAATGGGTGTCTTTGTG+CGG 0.682941 3_3:+63778480 Msa0464150:CDS
AAGTGATGATCATACAAAGG+GGG 0.720906 3_3:-63782012 None:intergenic
CAAGTGATGATCATACAAAG+GGG 0.746307 3_3:-63782013 None:intergenic
AGGGCCTTATGTGTTAACGG+TGG 0.764572 3_3:+63777868 Msa0464150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAATAATTTTTTATT+AGG + chr3_3:63779673-63779692 Msa0464150:intron 0.0%
!!! TTTTCTTTAAAGATATTTAA+AGG + chr3_3:63778387-63778406 Msa0464150:intron 10.0%
!!! TCATTTTCTTTATAAAATTT+TGG + chr3_3:63779570-63779589 Msa0464150:intron 10.0%
!! TCCTAATATATTAAAATTAA+AGG + chr3_3:63780485-63780504 Msa0464150:intron 10.0%
!!! ACCTTTAATTTTAATATATT+AGG - chr3_3:63780489-63780508 None:intergenic 10.0%
!! TATATATATGAAAAAAGTTA+GGG - chr3_3:63781149-63781168 None:intergenic 10.0%
!! ATATATATATGAAAAAAGTT+AGG - chr3_3:63781150-63781169 None:intergenic 10.0%
!!! ATTAAATAATTGTTGATTTT+TGG + chr3_3:63781335-63781354 Msa0464150:intron 10.0%
!!! TTATGCTTTATTTTAATATA+TGG + chr3_3:63781672-63781691 Msa0464150:intron 10.0%
!!! TATGCTTTATTTTAATATAT+GGG + chr3_3:63781673-63781692 Msa0464150:intron 10.0%
!! TGTAATTAAGATTATAAATT+TGG - chr3_3:63781719-63781738 None:intergenic 10.0%
!!! TTTTGGTAATAAAATGAATA+AGG + chr3_3:63777844-63777863 Msa0464150:CDS 15.0%
!!! TACTATCCATATTTTTTTTA+GGG - chr3_3:63778735-63778754 None:intergenic 15.0%
!! TTGAATTACTAACAAAAATT+AGG + chr3_3:63778911-63778930 Msa0464150:intron 15.0%
!! TGAATTACTAACAAAAATTA+GGG + chr3_3:63778912-63778931 Msa0464150:intron 15.0%
!! ATGCAAAAATAGTAAATAAT+TGG + chr3_3:63779641-63779660 Msa0464150:intron 15.0%
!! TGCAAAAATAGTAAATAATT+GGG + chr3_3:63779642-63779661 Msa0464150:intron 15.0%
!! TTATACATTTAAATAAGTGT+AGG - chr3_3:63779872-63779891 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr3_3:63780072-63780091 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr3_3:63780071-63780090 Msa0464150:intron 15.0%
!! AATGATTAAAAGAAAGAAAA+TGG - chr3_3:63780105-63780124 None:intergenic 15.0%
!! TATATTAAAATTAAAGGTCT+TGG + chr3_3:63780491-63780510 Msa0464150:intron 15.0%
!! AAACACTTTCATAAAAAAAA+GGG - chr3_3:63780868-63780887 None:intergenic 15.0%
!! AAAACACTTTCATAAAAAAA+AGG - chr3_3:63780869-63780888 None:intergenic 15.0%
!!! AAATAAAGCATAATTTTGAT+TGG - chr3_3:63781665-63781684 None:intergenic 15.0%
!!! ATGCTTTATTTTAATATATG+GGG + chr3_3:63781674-63781693 Msa0464150:intron 15.0%
!! TCAATGAATTTAAAAATTTC+AGG + chr3_3:63781907-63781926 Msa0464150:intron 15.0%
!! CAATGAATTTAAAAATTTCA+GGG + chr3_3:63781908-63781927 Msa0464150:intron 15.0%
!! GTCATTTATCAAATTATTAT+TGG - chr3_3:63782236-63782255 None:intergenic 15.0%
!!! AAAATAGGTGTTTCTAATTT+TGG + chr3_3:63777827-63777846 Msa0464150:CDS 20.0%
!! TTGTAAAACAGAATAAGTAA+GGG - chr3_3:63778042-63778061 None:intergenic 20.0%
!! ATTGTAAAACAGAATAAGTA+AGG - chr3_3:63778043-63778062 None:intergenic 20.0%
!! GTATGGAATTAAAATATTGA+CGG + chr3_3:63778131-63778150 Msa0464150:intron 20.0%
!! ATCATTCGATTAAGATTTAA+TGG - chr3_3:63778284-63778303 None:intergenic 20.0%
!!! TCTTTAAAGATATTTAAAGG+TGG + chr3_3:63778390-63778409 Msa0464150:intron 20.0%
!! TTTCTAACACTTTATAAGTA+TGG + chr3_3:63778703-63778722 Msa0464150:intron 20.0%
!!! ACTATCCATATTTTTTTTAG+GGG - chr3_3:63778734-63778753 None:intergenic 20.0%
!!! CTACTATCCATATTTTTTTT+AGG - chr3_3:63778736-63778755 None:intergenic 20.0%
!! TAAGAGAAATAAGTATTTCA+AGG + chr3_3:63778780-63778799 Msa0464150:intron 20.0%
!! AAGAGAAATAAGTATTTCAA+GGG + chr3_3:63778781-63778800 Msa0464150:intron 20.0%
!! ATGTTAGAAATCACAAAATT+AGG + chr3_3:63779140-63779159 Msa0464150:intron 20.0%
!! TTCAAAAGTTGTCATATAAA+TGG + chr3_3:63779597-63779616 Msa0464150:intron 20.0%
!! TGTAGGTCTATTATTAAAAA+GGG - chr3_3:63779825-63779844 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr3_3:63779985-63780004 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr3_3:63780062-63780081 Msa0464150:intron 20.0%
!!! TTTTTTTGGCATTAATTCTT+CGG - chr3_3:63780550-63780569 None:intergenic 20.0%
!! TTGATTATAACACAAGTTAA+TGG - chr3_3:63780583-63780602 None:intergenic 20.0%
!! AACACTTTCATAAAAAAAAG+GGG - chr3_3:63780867-63780886 None:intergenic 20.0%
!! TTATATAACCCAAACAAAAT+AGG - chr3_3:63781425-63781444 None:intergenic 20.0%
!! AGTTTACAAAAATATGGTTT+GGG - chr3_3:63781590-63781609 None:intergenic 20.0%
!! TAGTTTACAAAAATATGGTT+TGG - chr3_3:63781591-63781610 None:intergenic 20.0%
!!! AAACCATATTTTTGTAAACT+AGG + chr3_3:63781590-63781609 Msa0464150:intron 20.0%
!! TTAAATGCTGTATAAAATAC+TGG - chr3_3:63781992-63782011 None:intergenic 20.0%
!! ACATAATTAAATGTAATCAG+AGG + chr3_3:63782254-63782273 Msa0464150:intron 20.0%
! GTAATAAAATGAATAAGGCA+GGG + chr3_3:63777849-63777868 Msa0464150:CDS 25.0%
! AATGATTATCAATGTTTCCA+AGG + chr3_3:63778298-63778317 Msa0464150:intron 25.0%
! ATAGTCCCCTAAAAAAAATA+TGG + chr3_3:63778726-63778745 Msa0464150:intron 25.0%
! AAAACACTTATCGATAGAAT+TGG - chr3_3:63778956-63778975 None:intergenic 25.0%
!!! CTATCGATAAGTGTTTTTTT+TGG + chr3_3:63778959-63778978 Msa0464150:intron 25.0%
! TTACATCTAAAGAGAATAAG+TGG - chr3_3:63779086-63779105 None:intergenic 25.0%
! TTAAACACACAAAATTGAGT+CGG + chr3_3:63779238-63779257 Msa0464150:intron 25.0%
!! TATCATTTCTCTTTTGCATA+TGG - chr3_3:63779445-63779464 None:intergenic 25.0%
! TTTCTCTTTCTCTATTACTT+TGG + chr3_3:63779531-63779550 Msa0464150:intron 25.0%
! AAAAGGGAAATGTTAAGAAA+AGG - chr3_3:63779809-63779828 None:intergenic 25.0%
! GTGTAGGTCTATTATTAAAA+AGG - chr3_3:63779826-63779845 None:intergenic 25.0%
!! TTAAGTTCGAAAAAAAGTGT+AGG - chr3_3:63779842-63779861 None:intergenic 25.0%
!!! TTTAAAATGAGTGTCGTTTT+AGG - chr3_3:63780153-63780172 None:intergenic 25.0%
!!! AAACGACACTCATTTTAAAA+CGG + chr3_3:63780154-63780173 Msa0464150:intron 25.0%
! CGTAAAAAATTTGTCTCAAT+TGG + chr3_3:63780275-63780294 Msa0464150:intron 25.0%
!! GTACCATTTGGTATTATTTT+AGG + chr3_3:63780327-63780346 Msa0464150:intron 25.0%
! ATTTGGTTACCTGAAAATTA+CGG - chr3_3:63780456-63780475 None:intergenic 25.0%
!!! TTTTCAGGTAACCAAATTAT+AGG + chr3_3:63780459-63780478 Msa0464150:intron 25.0%
! TATTAGGAGAACCTATAATT+TGG - chr3_3:63780473-63780492 None:intergenic 25.0%
!!! ATGGTGGTATAAGATTTTTT+TGG - chr3_3:63780564-63780583 None:intergenic 25.0%
! ATTATAACACAAGTTAATGG+TGG - chr3_3:63780580-63780599 None:intergenic 25.0%
! TGTGTTATAATCAATGTATC+AGG + chr3_3:63780589-63780608 Msa0464150:intron 25.0%
!!! TTGATGATGATACTTTACAA+AGG + chr3_3:63780687-63780706 Msa0464150:CDS 25.0%
! AATAGAAAAGGTGAATTTGT+GGG - chr3_3:63780713-63780732 None:intergenic 25.0%
! TCAACCTTAATATGCTTAAT+TGG + chr3_3:63780997-63781016 Msa0464150:CDS 25.0%
!! TTTTTCATTGCATTTGCAAT+AGG - chr3_3:63781307-63781326 None:intergenic 25.0%
! ATTTAAACAGCTACTTCATT+TGG - chr3_3:63781563-63781582 None:intergenic 25.0%
! ATGAAGTAGCTGTTTAAATT+AGG + chr3_3:63781564-63781583 Msa0464150:intron 25.0%
! AAACGTTTATAAGAAAGAAG+AGG - chr3_3:63782099-63782118 None:intergenic 25.0%
!! TTTGTTGATTACAGTATCAT+TGG + chr3_3:63782289-63782308 Msa0464150:intron 25.0%
GGTAATAAAATGAATAAGGC+AGG + chr3_3:63777848-63777867 Msa0464150:CDS 30.0%
TTTCAAAAATGAGTGCAAAG+GGG - chr3_3:63778009-63778028 None:intergenic 30.0%
CTTTCAAAAATGAGTGCAAA+GGG - chr3_3:63778010-63778029 None:intergenic 30.0%
ACATGTTTCATTCACTAACT+TGG + chr3_3:63778081-63778100 Msa0464150:intron 30.0%
AGAAAAGTGGTAATTAGCAT+AGG - chr3_3:63778430-63778449 None:intergenic 30.0%
TACAACTTTGCAGGAAAATT+AGG + chr3_3:63778450-63778469 Msa0464150:intron 30.0%
!! ATTGATAACTAGCTCTACAT+AGG - chr3_3:63778544-63778563 None:intergenic 30.0%
TCACGTTAATATAACTCAGT+TGG + chr3_3:63779013-63779032 Msa0464150:intron 30.0%
! TTTTGCATATGGTCATAAGA+GGG - chr3_3:63779434-63779453 None:intergenic 30.0%
TTATTAGGTTTAAACCCACT+TGG + chr3_3:63779688-63779707 Msa0464150:intron 30.0%
TATTAGGTTTAAACCCACTT+GGG + chr3_3:63779689-63779708 Msa0464150:intron 30.0%
!! TTACTGATTCACTTTGTGTT+TGG - chr3_3:63780225-63780244 None:intergenic 30.0%
ATGTATTGTAACGTACCATT+TGG + chr3_3:63780315-63780334 Msa0464150:intron 30.0%
TGGCCTAAAATAATACCAAA+TGG - chr3_3:63780333-63780352 None:intergenic 30.0%
AGATCAAGGATTTACTTTCT+TGG + chr3_3:63780358-63780377 Msa0464150:CDS 30.0%
GATCAAGGATTTACTTTCTT+GGG + chr3_3:63780359-63780378 Msa0464150:CDS 30.0%
ATCAAGGATTTACTTTCTTG+GGG + chr3_3:63780360-63780379 Msa0464150:CDS 30.0%
AAATGTTAACATGTAGCCTT+AGG - chr3_3:63780527-63780546 None:intergenic 30.0%
GAATAGAAAAGGTGAATTTG+TGG - chr3_3:63780714-63780733 None:intergenic 30.0%
!!! CTGTTTTTGGAGAATAGAAA+AGG - chr3_3:63780725-63780744 None:intergenic 30.0%
! GTCATCTTTCTTCATTTTAC+AGG + chr3_3:63780830-63780849 Msa0464150:CDS 30.0%
AGCACCAATTAAGCATATTA+AGG - chr3_3:63781004-63781023 None:intergenic 30.0%
! GGGTATTTGAGATTAATAGA+GGG - chr3_3:63781129-63781148 None:intergenic 30.0%
! AGGGTATTTGAGATTAATAG+AGG - chr3_3:63781130-63781149 None:intergenic 30.0%
AAAGATTAGAATGTCTACCA+AGG + chr3_3:63781174-63781193 Msa0464150:intron 30.0%
ATATCTGATACGTCTCAAAT+AGG + chr3_3:63781263-63781282 Msa0464150:intron 30.0%
! TTTGGGTTATATAATCCGAA+CGG + chr3_3:63781431-63781450 Msa0464150:intron 30.0%
TTATAGGTGTTTATACCGTT+CGG - chr3_3:63781449-63781468 None:intergenic 30.0%
CAAAACACGTTAGAATGTTT+CGG + chr3_3:63781476-63781495 Msa0464150:intron 30.0%
!!! TCGGATTTTAGAATCTGAAT+AGG + chr3_3:63781495-63781514 Msa0464150:intron 30.0%
!!! CGGATTTTAGAATCTGAATA+GGG + chr3_3:63781496-63781515 Msa0464150:intron 30.0%
GTGCCTAGTTTACAAAAATA+TGG - chr3_3:63781596-63781615 None:intergenic 30.0%
ACAAGTGATGATCATACAAA+GGG - chr3_3:63782017-63782036 None:intergenic 30.0%
! TGATTTTATCAAACTCGTGA+TGG - chr3_3:63782169-63782188 None:intergenic 30.0%
CGAGTTTGATAAAATCAAGA+TGG + chr3_3:63782172-63782191 Msa0464150:intron 30.0%
TAGACATCAGATGAAGTAAT+TGG - chr3_3:63782436-63782455 None:intergenic 30.0%
TTCAAAAATGAGTGCAAAGG+GGG - chr3_3:63778008-63778027 None:intergenic 35.0%
CCTTTCAAAAATGAGTGCAA+AGG - chr3_3:63778011-63778030 None:intergenic 35.0%
!! CCTTTGCACTCATTTTTGAA+AGG + chr3_3:63778008-63778027 Msa0464150:CDS 35.0%
TAACTTGGATTCCATGTAGT+TGG + chr3_3:63778096-63778115 Msa0464150:intron 35.0%
AACTTGGATTCCATGTAGTT+GGG + chr3_3:63778097-63778116 Msa0464150:intron 35.0%
AATAAGTATATCCCGTTGTC+GGG - chr3_3:63778340-63778359 None:intergenic 35.0%
CAATAAGTATATCCCGTTGT+CGG - chr3_3:63778341-63778360 None:intergenic 35.0%
CTGCAAAGTTGTAAGAAAAG+TGG - chr3_3:63778443-63778462 None:intergenic 35.0%
! CACTTTTCTTACAACTTTGC+AGG + chr3_3:63778441-63778460 Msa0464150:intron 35.0%
AATTAGGCCAAAGATGACAA+TGG + chr3_3:63778466-63778485 Msa0464150:CDS 35.0%
ATTAGGCCAAAGATGACAAT+GGG + chr3_3:63778467-63778486 Msa0464150:CDS 35.0%
! CTTTTGCATATGGTCATAAG+AGG - chr3_3:63779435-63779454 None:intergenic 35.0%
GAATCAAATATGAGACCATG+AGG - chr3_3:63779757-63779776 None:intergenic 35.0%
GTCTCATATTTGATTCTCTC+TGG + chr3_3:63779761-63779780 Msa0464150:intron 35.0%
AAACGGATGGAGTATAATGT+GGG - chr3_3:63779902-63779921 None:intergenic 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr3_3:63779919-63779938 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - chr3_3:63780019-63780038 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - chr3_3:63780023-63780042 None:intergenic 35.0%
GTAAATCCTTGATCTATCAC+TGG - chr3_3:63780353-63780372 None:intergenic 35.0%
GAAGCATAGCAAAAGTGATT+GGG + chr3_3:63780640-63780659 Msa0464150:CDS 35.0%
!!! CCAGAATTATGTGCTGTTTT+TGG - chr3_3:63780738-63780757 None:intergenic 35.0%
CCAAAAACAGCACATAATTC+TGG + chr3_3:63780735-63780754 Msa0464150:CDS 35.0%
AGGAACACTCTTTCTAATGA+TGG + chr3_3:63780794-63780813 Msa0464150:CDS 35.0%
GGAACACTCTTTCTAATGAT+GGG + chr3_3:63780795-63780814 Msa0464150:CDS 35.0%
AAGTCTAAACTCAATCTCCT+TGG - chr3_3:63781194-63781213 None:intergenic 35.0%
TTCACAACATGACAGACAAA+TGG + chr3_3:63781212-63781231 Msa0464150:intron 35.0%
GACGTATCAGATATTAACTG+TGG - chr3_3:63781257-63781276 None:intergenic 35.0%
GGACACGCAATAAATCAATA+GGG - chr3_3:63781404-63781423 None:intergenic 35.0%
AGGACACGCAATAAATCAAT+AGG - chr3_3:63781405-63781424 None:intergenic 35.0%
! ATTGCGTGTCCTATTTTGTT+TGG + chr3_3:63781413-63781432 Msa0464150:intron 35.0%
!! TTGCGTGTCCTATTTTGTTT+GGG + chr3_3:63781414-63781433 Msa0464150:intron 35.0%
!!! CGTGTTTTGGAGGTTTTTAT+AGG - chr3_3:63781465-63781484 None:intergenic 35.0%
!! AACATTCTAACGTGTTTTGG+AGG - chr3_3:63781475-63781494 None:intergenic 35.0%
! CGAAACATTCTAACGTGTTT+TGG - chr3_3:63781478-63781497 None:intergenic 35.0%
ACAGCTACTTCATTTGGAAA+AGG - chr3_3:63781557-63781576 None:intergenic 35.0%
TTTCAGGGAATAATTACCTC+AGG + chr3_3:63781923-63781942 Msa0464150:intron 35.0%
AAGTGATGATCATACAAAGG+GGG - chr3_3:63782015-63782034 None:intergenic 35.0%
CAAGTGATGATCATACAAAG+GGG - chr3_3:63782016-63782035 None:intergenic 35.0%
CACAAGTGATGATCATACAA+AGG - chr3_3:63782018-63782037 None:intergenic 35.0%
ATGATCATCACTTGTGCTTT+AGG + chr3_3:63782022-63782041 Msa0464150:CDS 35.0%
TAGTGATCATCACTTGTGTT+CGG + chr3_3:63782127-63782146 Msa0464150:intron 35.0%
TTAACGGTGGCACTTCAATT+TGG + chr3_3:63777881-63777900 Msa0464150:CDS 40.0%
TAACGGTGGCACTTCAATTT+GGG + chr3_3:63777882-63777901 Msa0464150:CDS 40.0%
! AGGAGCATACATATTCTCCA+TGG + chr3_3:63777910-63777929 Msa0464150:CDS 40.0%
ATTCATTCCCATGTTGAGAC+TGG - chr3_3:63777936-63777955 None:intergenic 40.0%
! TGTAGAGTATCCCAACTACA+TGG - chr3_3:63778110-63778129 None:intergenic 40.0%
GTTGGGATACTCTACATGTA+TGG + chr3_3:63778114-63778133 Msa0464150:intron 40.0%
CAATGTTTCCAAGGAGTGAA+TGG + chr3_3:63778307-63778326 Msa0464150:intron 40.0%
AAGACACCCATTGTCATCTT+TGG - chr3_3:63778476-63778495 None:intergenic 40.0%
!! GATAATGGCGCTTGCATTTT+TGG + chr3_3:63778502-63778521 Msa0464150:CDS 40.0%
!!! AGTTTTAAGTCAACACCACG+AGG - chr3_3:63779719-63779738 None:intergenic 40.0%
TTTGAGTGTGTTCATCCTCA+TGG + chr3_3:63779739-63779758 Msa0464150:intron 40.0%
GAAACGGATGGAGTATAATG+TGG - chr3_3:63779903-63779922 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+TGG - chr3_3:63779915-63779934 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - chr3_3:63780020-63780039 None:intergenic 40.0%
!!! CGACACTCATTTTAAAACGG+AGG + chr3_3:63780157-63780176 Msa0464150:intron 40.0%
TTTAGGCCAGTGATAGATCA+AGG + chr3_3:63780344-63780363 Msa0464150:intron 40.0%
CTTGGGGATGCAATATACAT+CGG + chr3_3:63780376-63780395 Msa0464150:CDS 40.0%
GAAAATTACGGCTAGCACAA+AGG - chr3_3:63780444-63780463 None:intergenic 40.0%
! TTGTGCTAGCCGTAATTTTC+AGG + chr3_3:63780444-63780463 Msa0464150:CDS 40.0%
CGAAGCATAGCAAAAGTGAT+TGG + chr3_3:63780639-63780658 Msa0464150:CDS 40.0%
GGACATTAGTAACCTTTGCA+GGG + chr3_3:63780661-63780680 Msa0464150:CDS 40.0%
GACATTAGTAACCTTTGCAG+GGG + chr3_3:63780662-63780681 Msa0464150:CDS 40.0%
ACACGCAAACTCTCAAACAT+TGG + chr3_3:63780766-63780785 Msa0464150:CDS 40.0%
CACGCAAACTCTCAAACATT+GGG + chr3_3:63780767-63780786 Msa0464150:CDS 40.0%
!! CTTTCTAATGATGGGTTGTG+TGG + chr3_3:63780803-63780822 Msa0464150:CDS 40.0%
!! GTTGATAGTGACATTTCTGC+TGG - chr3_3:63780982-63781001 None:intergenic 40.0%
GGTTGGGACTTTAGACTATA+TGG + chr3_3:63781087-63781106 Msa0464150:CDS 40.0%
ACTATATGGTCCTCTCTACA+CGG + chr3_3:63781101-63781120 Msa0464150:CDS 40.0%
TCAAATAGGATGACCTCCAA+CGG + chr3_3:63781277-63781296 Msa0464150:intron 40.0%
CACGCAATAAATCAATAGGG+TGG - chr3_3:63781401-63781420 None:intergenic 40.0%
TGCGCGAGAAACTTATAAAG+TGG + chr3_3:63781519-63781538 Msa0464150:intron 40.0%
! GGATTGGTATTTCAAAGCAG+AGG + chr3_3:63781965-63781984 Msa0464150:CDS 40.0%
TTAGAAGTTACCTTCCCAAG+TGG - chr3_3:63782072-63782091 None:intergenic 40.0%
GAACACAAGTGATGATCACT+AGG - chr3_3:63782128-63782147 None:intergenic 40.0%
TAGACCCACTGCAATAATCA+GGG - chr3_3:63782317-63782336 None:intergenic 40.0%
ATAGACCCACTGCAATAATC+AGG - chr3_3:63782318-63782337 None:intergenic 40.0%
! TCAGTTTGCTTCATTGCAAC+TGG - chr3_3:63782403-63782422 None:intergenic 40.0%
AGTGCCACCGTTAACACATA+AGG - chr3_3:63777875-63777894 None:intergenic 45.0%
! GCACTTCAATTTGGGTTTGC+AGG + chr3_3:63777890-63777909 Msa0464150:CDS 45.0%
GATTCTCACCATTCACTCCT+TGG - chr3_3:63778318-63778337 None:intergenic 45.0%
AATGGTGAGAATCCCGACAA+CGG + chr3_3:63778325-63778344 Msa0464150:intron 45.0%
TGACAATGGGTGTCTTTGTG+CGG + chr3_3:63778480-63778499 Msa0464150:CDS 45.0%
AGGTTTAAACCCACTTGGGT+TGG + chr3_3:63779693-63779712 Msa0464150:intron 45.0%
GCTAGCACAAAGGTAACAGA+AGG - chr3_3:63780434-63780453 None:intergenic 45.0%
!! TCTTGGTAACGTGTGTCCTA+AGG + chr3_3:63780508-63780527 Msa0464150:intron 45.0%
GGGACATTAGTAACCTTTGC+AGG + chr3_3:63780660-63780679 Msa0464150:CDS 45.0%
ACTCTCAAACATTGGGTCTC+AGG + chr3_3:63780774-63780793 Msa0464150:CDS 45.0%
!! TAATGATGGGTTGTGTGGCT+TGG + chr3_3:63780808-63780827 Msa0464150:CDS 45.0%
!! TGGTGCTATGCAAACTAGTG+TGG + chr3_3:63781017-63781036 Msa0464150:CDS 45.0%
TGCTATGCAAACTAGTGTGG+TGG + chr3_3:63781020-63781039 Msa0464150:CDS 45.0%
!! TCACTCTGGTCTTGAGGTTT+GGG + chr3_3:63781059-63781078 Msa0464150:CDS 45.0%
TTGAGGTTTGGGCTATTGGT+TGG + chr3_3:63781070-63781089 Msa0464150:CDS 45.0%
! TGAGGTTTGGGCTATTGGTT+GGG + chr3_3:63781071-63781090 Msa0464150:CDS 45.0%
CAAATAGGATGACCTCCAAC+GGG + chr3_3:63781278-63781297 Msa0464150:intron 45.0%
AAATAGGATGACCTCCAACG+GGG + chr3_3:63781279-63781298 Msa0464150:intron 45.0%
CACATAGTACGCTATTCCTG+AGG - chr3_3:63781942-63781961 None:intergenic 45.0%
GGAATAGCGTACTATGTGCA+AGG + chr3_3:63781944-63781963 Msa0464150:CDS 45.0%
AGCGTACTATGTGCAAGGAT+TGG + chr3_3:63781949-63781968 Msa0464150:CDS 45.0%
GAGTTGCTCTGCAAAAAGGA+AGG - chr3_3:63782050-63782069 None:intergenic 45.0%
AGTGGAGTTGCTCTGCAAAA+AGG - chr3_3:63782054-63782073 None:intergenic 45.0%
! TTTTGCAGAGCAACTCCACT+TGG + chr3_3:63782054-63782073 Msa0464150:CDS 45.0%
TTTGCAGAGCAACTCCACTT+GGG + chr3_3:63782055-63782074 Msa0464150:CDS 45.0%
TGCAGTGGGTCTATATTCTG+TGG + chr3_3:63782324-63782343 Msa0464150:CDS 45.0%
TGGGTCTATATTCTGTGGTG+TGG + chr3_3:63782329-63782348 Msa0464150:CDS 45.0%
GGGTCTATATTCTGTGGTGT+GGG + chr3_3:63782330-63782349 Msa0464150:CDS 45.0%
GGTCTATATTCTGTGGTGTG+GGG + chr3_3:63782331-63782350 Msa0464150:CDS 45.0%
!! TTCATTGCAACTGGTGATGG+TGG - chr3_3:63782394-63782413 None:intergenic 45.0%
TGCTTCATTGCAACTGGTGA+TGG - chr3_3:63782397-63782416 None:intergenic 45.0%
!! AATATTACATTATATATATA+TGG + chr3_3:63779040-63779059 Msa0464150:intron 5.0%
! GGCAGGGCCTTATGTGTTAA+CGG + chr3_3:63777865-63777884 Msa0464150:CDS 50.0%
! AGGGCCTTATGTGTTAACGG+TGG + chr3_3:63777868-63777887 Msa0464150:CDS 50.0%
TCCATGGCCAGTCTCAACAT+GGG + chr3_3:63777926-63777945 Msa0464150:CDS 50.0%
AATGAGTGCAAAGGGGGCAA+GGG - chr3_3:63778002-63778021 None:intergenic 50.0%
AAATGAGTGCAAAGGGGGCA+AGG - chr3_3:63778003-63778022 None:intergenic 50.0%
ATGGTGAGAATCCCGACAAC+GGG + chr3_3:63778326-63778345 Msa0464150:intron 50.0%
GGGTGTCTTTGTGCGGATAA+TGG + chr3_3:63778487-63778506 Msa0464150:CDS 50.0%
GAGGGACTCAATTAGACGCT+TGG - chr3_3:63779416-63779435 None:intergenic 50.0%
CATCATCAATGCCCCTGCAA+AGG - chr3_3:63780676-63780695 None:intergenic 50.0%
TTCATTGCTGAGCGTCACTC+TGG + chr3_3:63781045-63781064 Msa0464150:CDS 50.0%
!! GTCACTCTGGTCTTGAGGTT+TGG + chr3_3:63781058-63781077 Msa0464150:CDS 50.0%
GGTCTTGAGGTTTGGGCTAT+TGG + chr3_3:63781066-63781085 Msa0464150:CDS 50.0%
!! ATAGAGGGTACCGTGTAGAG+AGG - chr3_3:63781114-63781133 None:intergenic 50.0%
ATTTGCAATAGGCTCCCCGT+TGG - chr3_3:63781296-63781315 None:intergenic 50.0%
AGATGGTCCTGTCACAGACA+CGG + chr3_3:63782189-63782208 Msa0464150:intron 50.0%
TCAATGTCCGTGTCTGTGAC+AGG - chr3_3:63782199-63782218 None:intergenic 50.0%
TGGAGCCCTGATTATTGCAG+TGG + chr3_3:63782309-63782328 Msa0464150:CDS 50.0%
GGAGCCCTGATTATTGCAGT+GGG + chr3_3:63782310-63782329 Msa0464150:CDS 50.0%
CTCCATGGCCAGTCTCAACA+TGG + chr3_3:63777925-63777944 Msa0464150:CDS 55.0%
! TCCCATGTTGAGACTGGCCA+TGG - chr3_3:63777930-63777949 None:intergenic 55.0%
!! TGAGCGTCACTCTGGTCTTG+AGG + chr3_3:63781053-63781072 Msa0464150:CDS 55.0%
CAGAGCAACTCCACTTGGGA+AGG + chr3_3:63782059-63782078 Msa0464150:CDS 55.0%
!! GCAACTGGTGATGGTGGCAT+AGG - chr3_3:63782388-63782407 None:intergenic 55.0%
ACCACGAGGCCAACCCAAGT+GGG - chr3_3:63779705-63779724 None:intergenic 60.0%
TGCAATAGGCTCCCCGTTGG+AGG - chr3_3:63781293-63781312 None:intergenic 60.0%
!! ACCCACTTGGGTTGGCCTCG+TGG + chr3_3:63779701-63779720 Msa0464150:intron 65.0%
CACCACGAGGCCAACCCAAG+TGG - chr3_3:63779706-63779725 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 63777821 63782456 63777821 ID=Msa0464150;Name=Msa0464150
chr3_3 mRNA 63777821 63782456 63777821 ID=Msa0464150-mRNA-1;Parent=Msa0464150;Name=Msa0464150-mRNA-1;_AED=0.39;_eAED=0.40;_QI=0|0|0|1|1|1|7|0|366
chr3_3 exon 63777821 63778029 63777821 ID=Msa0464150-mRNA-1:exon:14016;Parent=Msa0464150-mRNA-1
chr3_3 exon 63778463 63778525 63778463 ID=Msa0464150-mRNA-1:exon:14017;Parent=Msa0464150-mRNA-1
chr3_3 exon 63780349 63780465 63780349 ID=Msa0464150-mRNA-1:exon:14018;Parent=Msa0464150-mRNA-1
chr3_3 exon 63780611 63780851 63780611 ID=Msa0464150-mRNA-1:exon:14019;Parent=Msa0464150-mRNA-1
chr3_3 exon 63780958 63781122 63780958 ID=Msa0464150-mRNA-1:exon:14020;Parent=Msa0464150-mRNA-1
chr3_3 exon 63781929 63782080 63781929 ID=Msa0464150-mRNA-1:exon:14021;Parent=Msa0464150-mRNA-1
chr3_3 exon 63782303 63782456 63782303 ID=Msa0464150-mRNA-1:exon:14022;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63777821 63778029 63777821 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63778463 63778525 63778463 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63780349 63780465 63780349 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63780611 63780851 63780611 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63780958 63781122 63780958 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63781929 63782080 63781929 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
chr3_3 CDS 63782303 63782456 63782303 ID=Msa0464150-mRNA-1:cds;Parent=Msa0464150-mRNA-1
Gene Sequence

>Msa0464150

ATGGAAAAAATAGGTGTTTCTAATTTTGGTAATAAAATGAATAAGGCAGGGCCTTATGTGTTAACGGTGGCACTTCAATTTGGGTTTGCAGGAGCATACATATTCTCCATGGCCAGTCTCAACATGGGAATGAATCGTTTTGTGTTCATTGTTTATCGTAATCTCATTGCTGCAATTGCCCTTGCCCCCTTTGCACTCATTTTTGAAAGGAAAATTAGGCCAAAGATGACAATGGGTGTCTTTGTGCGGATAATGGCGCTTGCATTTTTGGAGCCAGTGATAGATCAAGGATTTACTTTCTTGGGGATGCAATATACATCGGCTTCATTTGCTTCTGCTTTGATGAATGCTGTTCCTTCTGTTACCTTTGTGCTAGCCGTAATTTTCAGGATTGAACGTGTAAATATGAAAGAGATACGAAGCATAGCAAAAGTGATTGGGACATTAGTAACCTTTGCAGGGGCATTGATGATGATACTTTACAAAGGCCCACAAATTCACCTTTTCTATTCTCCAAAAACAGCACATAATTCTGGAAGTCACGACACGCAAACTCTCAAACATTGGGTCTCAGGAACACTCTTTCTAATGATGGGTTGTGTGGCTTGGTCATCTTTCTTCATTTTACAGTCAGTAACGTTGAAAAAGTATCCAGCAGAAATGTCACTATCAACCTTAATATGCTTAATTGGTGCTATGCAAACTAGTGTGGTGGCATTCATTGCTGAGCGTCACTCTGGTCTTGAGGTTTGGGCTATTGGTTGGGACTTTAGACTATATGGTCCTCTCTACACGGGAATAATTACCTCAGGAATAGCGTACTATGTGCAAGGATTGGTATTTCAAAGCAGAGGACCAGTATTTTATACAGCATTTAACCCCCTTTGTATGATCATCACTTGTGCTTTAGGTTCCTTCCTTTTTGCAGAGCAACTCCACTTGGGAAGTATCATTGGAGCCCTGATTATTGCAGTGGGTCTATATTCTGTGGTGTGGGGTAAAGCAAAAGACTATTCATCAGACACTACTCCTATGCCACCATCACCAGTTGCAATGAAGCAAACTGAGAAACAACTTCCAATTACTTCATCTGATGTCTAA
Protein sequence

>Msa0464150

MEKIGVSNFGNKMNKAGPYVLTVALQFGFAGAYIFSMASLNMGMNRFVFIVYRNLIAAIALAPFALIFERKIRPKMTMGVFVRIMALAFLEPVIDQGFTFLGMQYTSASFASALMNAVPSVTFVLAVIFRIERVNMKEIRSIAKVIGTLVTFAGALMMILYKGPQIHLFYSPKTAHNSGSHDTQTLKHWVSGTLFLMMGCVAWSSFFILQSVTLKKYPAEMSLSTLICLIGAMQTSVVAFIAERHSGLEVWAIGWDFRLYGPLYTGIITSGIAYYVQGLVFQSRGPVFYTAFNPLCMIITCALGSFLFAEQLHLGSIIGALIIAVGLYSVVWGKAKDYSSDTTPMPPSPVAMKQTEKQLPITSSDV*