AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0655910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0655910 MtrunA17_Chr8g0375991 97.642 212 5 0 1 212 1 212 2.30e-152 421
Msa0655910 MtrunA17_Chr1g0161941 59.048 210 84 2 1 208 1 210 3.92e-85 251
Msa0655910 MtrunA17_Chr1g0161921 43.750 208 114 3 1 206 7 213 4.64e-57 180
Msa0655910 MtrunA17_Chr1g0203371 39.604 202 117 2 9 205 13 214 2.31e-53 171
Msa0655910 MtrunA17_Chr6g0456901 36.869 198 119 3 11 202 16 213 2.49e-40 137
Msa0655910 MtrunA17_Chr5g0422371 35.079 191 111 6 10 189 17 205 1.21e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0655910 AT1G14730.1 63.107 206 73 3 6 209 17 221 2.53e-88 259
Msa0655910 AT4G25570.1 38.571 210 124 2 1 205 5 214 8.06e-50 162
Msa0655910 AT4G25570.2 38.571 210 124 2 1 205 46 255 1.01e-48 160
Msa0655910 AT5G38630.1 37.073 205 123 3 8 206 13 217 1.28e-43 146
Msa0655910 AT1G26100.1 34.104 173 102 5 8 177 13 176 9.33e-24 95.1

Find 110 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTATAGTACCTCAATTT+TGG 0.192371 4_3:+64874333 Msa0655910:CDS
GCCTTGAACACGTGCGCTTC+TGG 0.194316 4_3:+64876781 Msa0655910:three_prime_UTR
CATTTCATTGCCATAGTTCT+TGG 0.208807 4_3:+64875418 Msa0655910:CDS
ATCCGTTCTCCCTCGATGTT+TGG 0.214221 4_3:-64876900 None:intergenic
TTCATAATTAAAATACAAAT+TGG 0.236533 4_3:+64876329 Msa0655910:three_prime_UTR
CCCTCATCCTTATGCTTGTT+TGG 0.262566 4_3:+64874377 Msa0655910:CDS
GGTCTAGCAATTCTCTTGTT+TGG 0.264168 4_3:+64876059 Msa0655910:CDS
CGAGAACATTCTGACGCATT+TGG 0.304248 4_3:-64877185 None:intergenic
ATGTTTACAGCCTCCATTCA+TGG 0.312973 4_3:+64875494 Msa0655910:CDS
GTGTTCAAGGCGGATCTTCC+AGG 0.314703 4_3:-64876769 None:intergenic
ATTGCCTGAGAATGCTAAAA+TGG 0.321169 4_3:-64876602 None:intergenic
GCAATTTGTGCTGCACTAAC+AGG 0.327192 4_3:+64875966 Msa0655910:CDS
TCCGTTCTCCCTCGATGTTT+GGG 0.332909 4_3:-64876899 None:intergenic
GCCATAGTTCTTGGAATTGT+TGG 0.333746 4_3:+64875427 Msa0655910:CDS
GAATGTGATGAGGTATTTAA+AGG 0.336142 4_3:+64877101 Msa0655910:three_prime_UTR
AAGGAAGAGGAAAGTGAATT+GGG 0.341493 4_3:+64876853 Msa0655910:three_prime_UTR
TGATGAGACGGGATTCTCTT+TGG 0.342183 4_3:-64876028 None:intergenic
TAAATGCAAAAGCTAACTAT+AGG 0.353480 4_3:-64874267 None:intergenic
CTCCATTCATGGATTGGCCT+TGG 0.364175 4_3:+64875505 Msa0655910:CDS
AGGTAGAGATTGAAGATGAT+AGG 0.379460 4_3:-64876726 None:intergenic
TGCTGCACTAACAGGTTTGA+TGG 0.381294 4_3:+64875974 Msa0655910:CDS
ACTTCCTCCTTCTTCAGTCC+TGG 0.388312 4_3:+64876751 Msa0655910:three_prime_UTR
ACCAACAATTCCAAGAACTA+TGG 0.394185 4_3:-64875428 None:intergenic
TACAGCCTCCATTCATGGAT+TGG 0.402679 4_3:+64875499 Msa0655910:CDS
CCTCTGCCACCGAAGAAACT+TGG 0.402818 4_3:+64876943 Msa0655910:three_prime_UTR
GTAATAAAGAGAATGTAATT+TGG 0.407285 4_3:-64876400 None:intergenic
TTCTGTGTTTCATGTGTGCC+TGG 0.408328 4_3:-64875376 None:intergenic
TGTGGATATGACAGTTGGTT+TGG 0.414085 4_3:+64876088 Msa0655910:CDS
GGCTTCTTAATCTCTTCCTT+AGG 0.419191 4_3:+64876532 Msa0655910:three_prime_UTR
GAAGGAAGAGGAAAGTGAAT+TGG 0.420609 4_3:+64876852 Msa0655910:three_prime_UTR
GGACTGACATTCATGTTTCA+AGG 0.423824 4_3:+64875876 Msa0655910:CDS
TGTTGTTCATTAAAATGAAA+TGG 0.426492 4_3:+64876240 Msa0655910:three_prime_UTR
TCGCTGATTCCAAGTTTCTT+CGG 0.431698 4_3:-64876952 None:intergenic
GTTAAGACCATTGATATCTC+TGG 0.432223 4_3:-64876505 None:intergenic
ATATCAATGGTCTTAACTTC+AGG 0.435827 4_3:+64876511 Msa0655910:three_prime_UTR
AAATGCAAAAGCTAACTATA+GGG 0.438376 4_3:-64874266 None:intergenic
AACCCAAAGAACATCAGAAA+AGG 0.444479 4_3:-64874593 None:intergenic
CGATGTTTGGGATCCATTCA+AGG 0.446277 4_3:-64876887 None:intergenic
TAGAAAATTCTATAACTGTC+TGG 0.452298 4_3:+64877150 Msa0655910:three_prime_UTR
TCCAGAAGCGCACGTGTTCA+AGG 0.460352 4_3:-64876782 None:intergenic
CCAAGTTTCTTCGGTGGCAG+AGG 0.488800 4_3:-64876943 None:intergenic
GGTAGAGATTGAAGATGATA+GGG 0.493419 4_3:-64876725 None:intergenic
TCGCAAGCACACGTGTGGAC+TGG 0.494481 4_3:-64876813 None:intergenic
TGTATAAGAAGATAGATTTG+AGG 0.499759 4_3:-64876144 None:intergenic
GAGGTATTTAAAGGAAATAG+AGG 0.500432 4_3:+64877110 Msa0655910:three_prime_UTR
TCGTCCGTCCAAGAAGTTGA+AGG 0.501844 4_3:-64876982 None:intergenic
CCTCACTGTGACTGTGCCTA+AGG 0.507634 4_3:-64876548 None:intergenic
GTAGCAGTGGAAAGTTTATG+AGG 0.516165 4_3:-64876633 None:intergenic
CTGCAAGCCAAACAAGCAAA+AGG 0.518582 4_3:-64875530 None:intergenic
GCAGCAACCAAACAAGCATA+AGG 0.521345 4_3:-64874384 None:intergenic
CAACCAAGTTCACCATATCA+TGG 0.523417 4_3:-64875471 None:intergenic
AAGAAGACAAGAATGATCAA+TGG 0.523834 4_3:-64876669 None:intergenic
AAGACAATCAAGGGCTGCAA+TGG 0.529021 4_3:+64875902 Msa0655910:CDS
TGTTTAACCAGAGATATCAA+TGG 0.529039 4_3:+64876498 Msa0655910:three_prime_UTR
ACCAGTAAAATTGATGAGAC+GGG 0.529605 4_3:-64876039 None:intergenic
ATGTCTTACCACACAGTACC+AGG 0.530006 4_3:+64875358 Msa0655910:CDS
CATATCATGGAACTTGAAAA+CGG 0.532163 4_3:-64875458 None:intergenic
CACTAACAGGTTTGATGGAG+AGG 0.533891 4_3:+64875979 Msa0655910:CDS
GACCAGTAAAATTGATGAGA+CGG 0.537528 4_3:-64876040 None:intergenic
TTCAAGGAACAAGACAATCA+AGG 0.538249 4_3:+64875892 Msa0655910:CDS
GGATCTTCCAGGACTGAAGA+AGG 0.540913 4_3:-64876758 None:intergenic
TGCCAAGGCCAATCCATGAA+TGG 0.541924 4_3:-64875507 None:intergenic
ATGGCTCCATGGCACATAGC+TGG 0.542007 4_3:+64875921 Msa0655910:CDS
GTTCCATGATATGGTGAACT+TGG 0.544949 4_3:+64875468 Msa0655910:CDS
TCTTCGGTGGCAGAGGCAGA+AGG 0.547448 4_3:-64876936 None:intergenic
ACCAAACAAGCATAAGGATG+AGG 0.552123 4_3:-64874378 None:intergenic
AGTTCTGTGTTATGTTAGAA+TGG 0.552597 4_3:-64876467 None:intergenic
CAAGGGCTGCAATGGCTCCA+TGG 0.553808 4_3:+64875910 Msa0655910:CDS
ACTATAAGAACGCATCCTGC+TGG 0.555133 4_3:-64874320 None:intergenic
ACCCAAAGAACATCAGAAAA+GGG 0.558431 4_3:-64874592 None:intergenic
GGGAATGGAAGATCCTTGAA+TGG 0.560868 4_3:+64876874 Msa0655910:three_prime_UTR
GGATCAAGTGAAAGCTGCAA+TGG 0.563638 4_3:-64876581 None:intergenic
CAAACAAGCAAAAGGTGCCA+AGG 0.564883 4_3:-64875522 None:intergenic
TCCCAAACATCGAGGGAGAA+CGG 0.569708 4_3:+64876898 Msa0655910:three_prime_UTR
ACATTCTGACGCATTTGGCG+CGG 0.570484 4_3:-64877180 None:intergenic
CTACCACCAGCTATGTGCCA+TGG 0.572574 4_3:-64875927 None:intergenic
GAGGAAAGTGAATTGGGGAA+TGG 0.573543 4_3:+64876859 Msa0655910:three_prime_UTR
TCAAGGAACAAGACAATCAA+GGG 0.574140 4_3:+64875893 Msa0655910:CDS
GAGGGGATTGAATATGACTC+TGG 0.574307 4_3:+64874415 Msa0655910:CDS
TGGTTGCTGCATTACCGTGA+GGG 0.574869 4_3:+64874397 Msa0655910:CDS
CTGATTCCAAGTTTCTTCGG+TGG 0.575427 4_3:-64876949 None:intergenic
ACTGAAGAAGGAGGAAGTGA+AGG 0.575523 4_3:-64876746 None:intergenic
GTGAAAGCTGCAATGGAGAA+TGG 0.576830 4_3:-64876574 None:intergenic
GGTTGCTGCATTACCGTGAG+GGG 0.582408 4_3:+64874398 Msa0655910:CDS
CGTATACTGTAGTAACCAGC+AGG 0.585010 4_3:+64874305 Msa0655910:five_prime_UTR
TGCACTCGCAAGCACACGTG+TGG 0.585011 4_3:-64876818 None:intergenic
CATCGCGTGGAGCGTAGCAG+TGG 0.585473 4_3:-64876646 None:intergenic
AGACGGGATTCTCTTTGGTG+TGG 0.594078 4_3:-64876023 None:intergenic
CATACATTGAAAACACGAAG+TGG 0.594783 4_3:-64874442 None:intergenic
AAACAAGTGAGCCAAAATTG+AGG 0.600449 4_3:-64874344 None:intergenic
TATAAGTTGATTGATTCATG+TGG 0.605545 4_3:+64876295 Msa0655910:three_prime_UTR
CCAAACAAGCATAAGGATGA+GGG 0.611153 4_3:-64874377 None:intergenic
AAGCACACGTGTGGACTGGA+AGG 0.615381 4_3:-64876809 None:intergenic
GAATGGATCCCAAACATCGA+GGG 0.616429 4_3:+64876891 Msa0655910:three_prime_UTR
GCAGTGGAAAGTTTATGAGG+AGG 0.629631 4_3:-64876630 None:intergenic
GAATGATCAATGGCATCGCG+TGG 0.629958 4_3:-64876659 None:intergenic
AGGAAGAGGAAAGTGAATTG+GGG 0.634584 4_3:+64876854 Msa0655910:three_prime_UTR
CAAGGCCAATCCATGAATGG+AGG 0.654155 4_3:-64875504 None:intergenic
AGTCATATTCAATCCCCTCA+CGG 0.654951 4_3:-64874411 None:intergenic
AATATATACCTTGACCAACA+AGG 0.656313 4_3:-64874622 None:intergenic
TCTTCCAGGACTGAAGAAGG+AGG 0.671818 4_3:-64876755 None:intergenic
GCTCCATGGCACATAGCTGG+TGG 0.673506 4_3:+64875924 Msa0655910:CDS
TTCAAGAGGAGAATGTGATG+AGG 0.680858 4_3:+64877091 Msa0655910:three_prime_UTR
TTGGTTGCTGCATTACCGTG+AGG 0.689331 4_3:+64874396 Msa0655910:CDS
TGAATGGATCCCAAACATCG+AGG 0.698989 4_3:+64876890 Msa0655910:three_prime_UTR
GATAGGGTTCTTCAGATCAG+CGG 0.712601 4_3:-64876709 None:intergenic
CATGTGTGCCTGGTACTGTG+TGG 0.717385 4_3:-64875366 None:intergenic
CCTTAGGCACAGTCACAGTG+AGG 0.723797 4_3:+64876548 Msa0655910:three_prime_UTR
AGAAGCGCACGTGTTCAAGG+CGG 0.739000 4_3:-64876779 None:intergenic
GCTGGTTACTACAGTATACG+AGG 0.741619 4_3:-64874302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTAGCTTTAAAATATA+AGG - chr4_3:64875028-64875047 None:intergenic 10.0%
!! TTCATAATTAAAATACAAAT+TGG + chr4_3:64876329-64876348 Msa0655910:three_prime_UTR 10.0%
!!! AATTTTTTATACTTAACAAC+TGG + chr4_3:64874490-64874509 Msa0655910:intron 15.0%
!!! AATTTTTTATACTTAACAAC+TGG + chr4_3:64874490-64874509 Msa0655910:intron 15.0%
!!! TTTATTTCAAGTTTTAAGAT+AGG + chr4_3:64874646-64874665 Msa0655910:intron 15.0%
!! TAAGAAACAAAGTAAAAATT+AGG + chr4_3:64875206-64875225 Msa0655910:intron 15.0%
!! AAGAAACAAAGTAAAAATTA+GGG + chr4_3:64875207-64875226 Msa0655910:intron 15.0%
!! TAAATTCAAATGTTATTGTA+GGG - chr4_3:64875568-64875587 None:intergenic 15.0%
!! TTAAATTCAAATGTTATTGT+AGG - chr4_3:64875569-64875588 None:intergenic 15.0%
!! TAAGAAATATGCAAATTCTA+TGG - chr4_3:64874995-64875014 None:intergenic 20.0%
!! TTTACAGTTAAAATAGAAAC+TGG + chr4_3:64875235-64875254 Msa0655910:intron 20.0%
!! TATTTCAGTTTACTAGTATT+AGG - chr4_3:64875276-64875295 None:intergenic 20.0%
!!! AATATAAACTATTTTTGCAG+TGG + chr4_3:64875847-64875866 Msa0655910:intron 20.0%
!! ACTTCAAGAAAAAACAATAT+GGG - chr4_3:64876114-64876133 None:intergenic 20.0%
!! ACATATAAAAAGAAGAATGT+TGG - chr4_3:64876201-64876220 None:intergenic 20.0%
!! TGTTGTTCATTAAAATGAAA+TGG + chr4_3:64876240-64876259 Msa0655910:three_prime_UTR 20.0%
!! GTAATAAAGAGAATGTAATT+TGG - chr4_3:64876403-64876422 None:intergenic 20.0%
!! TGTTATGACACAAAAGTTTT+TGG - chr4_3:64874242-64874261 None:intergenic 25.0%
! AAATGCAAAAGCTAACTATA+GGG - chr4_3:64874269-64874288 None:intergenic 25.0%
! TAAATGCAAAAGCTAACTAT+AGG - chr4_3:64874270-64874289 None:intergenic 25.0%
!! TTCTTATAGTACCTCAATTT+TGG + chr4_3:64874333-64874352 Msa0655910:CDS 25.0%
!!! ATTTCAAGTTTTAAGATAGG+TGG + chr4_3:64874649-64874668 Msa0655910:intron 25.0%
!!! TGTAAACTTCTACTTTTCAA+AGG - chr4_3:64874720-64874739 None:intergenic 25.0%
!!! TTCAAACGATTTTGAAGTAT+TGG + chr4_3:64874944-64874963 Msa0655910:intron 25.0%
!!! TTTTGAAGTATTGGTCTATT+TGG + chr4_3:64874953-64874972 Msa0655910:intron 25.0%
! AATTACTTGACTGATTGAAT+TGG + chr4_3:64875311-64875330 Msa0655910:intron 25.0%
! TTGAATTTAACCAAAAAGCA+TGG + chr4_3:64875579-64875598 Msa0655910:intron 25.0%
! TAAATTTAATAGAATGTCGC+TGG - chr4_3:64875806-64875825 None:intergenic 25.0%
! CACTTCAAGAAAAAACAATA+TGG - chr4_3:64876115-64876134 None:intergenic 25.0%
! TGTATAAGAAGATAGATTTG+AGG - chr4_3:64876147-64876166 None:intergenic 25.0%
!!! TATAAGTTGATTGATTCATG+TGG + chr4_3:64876295-64876314 Msa0655910:three_prime_UTR 25.0%
!!! TTATTTTGATGGTTTTCAAG+AGG + chr4_3:64877077-64877096 Msa0655910:three_prime_UTR 25.0%
! TAGAAAATTCTATAACTGTC+TGG + chr4_3:64877150-64877169 Msa0655910:three_prime_UTR 25.0%
! TGAATTTTTCTCCATTATGC+AGG + chr4_3:64874565-64874584 Msa0655910:intron 30.0%
AATATATACCTTGACCAACA+AGG - chr4_3:64874625-64874644 None:intergenic 30.0%
ATATTTACATCGAGACATTG+AGG + chr4_3:64874743-64874762 Msa0655910:intron 30.0%
! GTTCTTCTTGTTATAACATG+TGG - chr4_3:64874878-64874897 None:intergenic 30.0%
! ACTTTGGAAGTAGAAGTTTA+TGG - chr4_3:64874900-64874919 None:intergenic 30.0%
AAATCGTTTGAAAACCTGAA+TGG - chr4_3:64874936-64874955 None:intergenic 30.0%
GAAATATGCAAATTCTATGG+TGG - chr4_3:64874992-64875011 None:intergenic 30.0%
AGTCAAGTAATTGATATCTG+AGG - chr4_3:64875303-64875322 None:intergenic 30.0%
CATATCATGGAACTTGAAAA+CGG - chr4_3:64875461-64875480 None:intergenic 30.0%
AGTTCTGTGTTATGTTAGAA+TGG - chr4_3:64876470-64876489 None:intergenic 30.0%
TGTTTAACCAGAGATATCAA+TGG + chr4_3:64876498-64876517 Msa0655910:three_prime_UTR 30.0%
! ATATCAATGGTCTTAACTTC+AGG + chr4_3:64876511-64876530 Msa0655910:three_prime_UTR 30.0%
AAGAAGACAAGAATGATCAA+TGG - chr4_3:64876672-64876691 None:intergenic 30.0%
! ACATTTTTCCTTCAACTTCT+TGG + chr4_3:64876974-64876993 Msa0655910:three_prime_UTR 30.0%
! GAATGTGATGAGGTATTTAA+AGG + chr4_3:64877101-64877120 Msa0655910:three_prime_UTR 30.0%
! GAGGTATTTAAAGGAAATAG+AGG + chr4_3:64877110-64877129 Msa0655910:three_prime_UTR 30.0%
!! TAGAATTTTCTAGCAGAAAC+TGG - chr4_3:64877142-64877161 None:intergenic 30.0%
AAACAAGTGAGCCAAAATTG+AGG - chr4_3:64874347-64874366 None:intergenic 35.0%
!!! TCAATTTTGGCTCACTTGTT+TGG + chr4_3:64874346-64874365 Msa0655910:CDS 35.0%
CATACATTGAAAACACGAAG+TGG - chr4_3:64874445-64874464 None:intergenic 35.0%
! ACCCTTTTCTGATGTTCTTT+GGG + chr4_3:64874591-64874610 Msa0655910:CDS 35.0%
ACCCAAAGAACATCAGAAAA+GGG - chr4_3:64874595-64874614 None:intergenic 35.0%
AACCCAAAGAACATCAGAAA+AGG - chr4_3:64874596-64874615 None:intergenic 35.0%
!! TTTGGGTTCATTTTCCTTGT+TGG + chr4_3:64874608-64874627 Msa0655910:CDS 35.0%
! TTCATTTTCCTTGTTGGTCA+AGG + chr4_3:64874614-64874633 Msa0655910:CDS 35.0%
! TTTCAAGTAATGATCCACGT+CGG - chr4_3:64874676-64874695 None:intergenic 35.0%
! ATTGGTCTATTTGGTCACTA+TGG + chr4_3:64874962-64874981 Msa0655910:intron 35.0%
CATTTCATTGCCATAGTTCT+TGG + chr4_3:64875418-64875437 Msa0655910:CDS 35.0%
ACCAACAATTCCAAGAACTA+TGG - chr4_3:64875431-64875450 None:intergenic 35.0%
!!! CGTTTTCAAGTTCCATGATA+TGG + chr4_3:64875459-64875478 Msa0655910:CDS 35.0%
! TTGTTCAAAGCCATGCTTTT+TGG - chr4_3:64875592-64875611 None:intergenic 35.0%
!!! TGTCGCTGGTTAGTTTTTTA+GGG - chr4_3:64875792-64875811 None:intergenic 35.0%
!!! ATGTCGCTGGTTAGTTTTTT+AGG - chr4_3:64875793-64875812 None:intergenic 35.0%
!!! CTATTTTTGCAGTGGCTTTT+TGG + chr4_3:64875855-64875874 Msa0655910:intron 35.0%
TTCAAGGAACAAGACAATCA+AGG + chr4_3:64875892-64875911 Msa0655910:CDS 35.0%
TCAAGGAACAAGACAATCAA+GGG + chr4_3:64875893-64875912 Msa0655910:CDS 35.0%
!! TGGTAGAGCACTATTTTTCA+TGG + chr4_3:64875944-64875963 Msa0655910:CDS 35.0%
ACCAGTAAAATTGATGAGAC+GGG - chr4_3:64876042-64876061 None:intergenic 35.0%
GACCAGTAAAATTGATGAGA+CGG - chr4_3:64876043-64876062 None:intergenic 35.0%
!! TCTCTTGTTTGGAGTTTTTG+TGG + chr4_3:64876070-64876089 Msa0655910:CDS 35.0%
! GTTTTTGTGGATATGACAGT+TGG + chr4_3:64876083-64876102 Msa0655910:CDS 35.0%
GTTAAGACCATTGATATCTC+TGG - chr4_3:64876508-64876527 None:intergenic 35.0%
!! TGATCCATTTTAGCATTCTC+AGG + chr4_3:64876598-64876617 Msa0655910:three_prime_UTR 35.0%
ATTGCCTGAGAATGCTAAAA+TGG - chr4_3:64876605-64876624 None:intergenic 35.0%
GGTAGAGATTGAAGATGATA+GGG - chr4_3:64876728-64876747 None:intergenic 35.0%
AGGTAGAGATTGAAGATGAT+AGG - chr4_3:64876729-64876748 None:intergenic 35.0%
AAGGAAGAGGAAAGTGAATT+GGG + chr4_3:64876853-64876872 Msa0655910:three_prime_UTR 35.0%
! TTTTCCTTCAACTTCTTGGA+CGG + chr4_3:64876978-64876997 Msa0655910:three_prime_UTR 35.0%
CCAAACAAGCATAAGGATGA+GGG - chr4_3:64874380-64874399 None:intergenic 40.0%
ACCAAACAAGCATAAGGATG+AGG - chr4_3:64874381-64874400 None:intergenic 40.0%
AGTCATATTCAATCCCCTCA+CGG - chr4_3:64874414-64874433 None:intergenic 40.0%
AAAAGGGTGAACCTGCATAA+TGG - chr4_3:64874579-64874598 None:intergenic 40.0%
! CACCCTTTTCTGATGTTCTT+TGG + chr4_3:64874590-64874609 Msa0655910:CDS 40.0%
! GACATTGAGGTGTTTGATGT+CGG + chr4_3:64874756-64874775 Msa0655910:intron 40.0%
TGGTAAGTGATGTTGCACTT+TGG - chr4_3:64874916-64874935 None:intergenic 40.0%
TGCAACATCACTTACCATTC+AGG + chr4_3:64874919-64874938 Msa0655910:intron 40.0%
GCCATAGTTCTTGGAATTGT+TGG + chr4_3:64875427-64875446 Msa0655910:CDS 40.0%
GTTCCATGATATGGTGAACT+TGG + chr4_3:64875468-64875487 Msa0655910:CDS 40.0%
CAACCAAGTTCACCATATCA+TGG - chr4_3:64875474-64875493 None:intergenic 40.0%
ATGTTTACAGCCTCCATTCA+TGG + chr4_3:64875494-64875513 Msa0655910:CDS 40.0%
TGTCCAAAAGATGTAGCGAT+GGG + chr4_3:64875702-64875721 Msa0655910:intron 40.0%
TACGCTGAAAGACTTTCAGA+AGG + chr4_3:64875729-64875748 Msa0655910:intron 40.0%
GGACTGACATTCATGTTTCA+AGG + chr4_3:64875876-64875895 Msa0655910:CDS 40.0%
! TCCCGTCTCATCAATTTTAC+TGG + chr4_3:64876038-64876057 Msa0655910:CDS 40.0%
GGTCTAGCAATTCTCTTGTT+TGG + chr4_3:64876059-64876078 Msa0655910:CDS 40.0%
TGTGGATATGACAGTTGGTT+TGG + chr4_3:64876088-64876107 Msa0655910:CDS 40.0%
GGCTTCTTAATCTCTTCCTT+AGG + chr4_3:64876532-64876551 Msa0655910:three_prime_UTR 40.0%
GTAGCAGTGGAAAGTTTATG+AGG - chr4_3:64876636-64876655 None:intergenic 40.0%
! TTGCGAGTGCAGAATTTTCT+GGG + chr4_3:64876830-64876849 Msa0655910:three_prime_UTR 40.0%
!! AGAATTTTCTGGGAAGGAAG+AGG + chr4_3:64876840-64876859 Msa0655910:three_prime_UTR 40.0%
GAAGGAAGAGGAAAGTGAAT+TGG + chr4_3:64876852-64876871 Msa0655910:three_prime_UTR 40.0%
AGGAAGAGGAAAGTGAATTG+GGG + chr4_3:64876854-64876873 Msa0655910:three_prime_UTR 40.0%
!! TCGCTGATTCCAAGTTTCTT+CGG - chr4_3:64876955-64876974 None:intergenic 40.0%
TTCAAGAGGAGAATGTGATG+AGG + chr4_3:64877091-64877110 Msa0655910:three_prime_UTR 40.0%
GCTGGTTACTACAGTATACG+AGG - chr4_3:64874305-64874324 None:intergenic 45.0%
CGTATACTGTAGTAACCAGC+AGG + chr4_3:64874305-64874324 Msa0655910:five_prime_UTR 45.0%
ACTATAAGAACGCATCCTGC+TGG - chr4_3:64874323-64874342 None:intergenic 45.0%
CCCTCATCCTTATGCTTGTT+TGG + chr4_3:64874377-64874396 Msa0655910:CDS 45.0%
GCAGCAACCAAACAAGCATA+AGG - chr4_3:64874387-64874406 None:intergenic 45.0%
GAGGGGATTGAATATGACTC+TGG + chr4_3:64874415-64874434 Msa0655910:CDS 45.0%
ATGTCTTACCACACAGTACC+AGG + chr4_3:64875358-64875377 Msa0655910:CDS 45.0%
TTCTGTGTTTCATGTGTGCC+TGG - chr4_3:64875379-64875398 None:intergenic 45.0%
TACAGCCTCCATTCATGGAT+TGG + chr4_3:64875499-64875518 Msa0655910:CDS 45.0%
!! CAAACAAGCAAAAGGTGCCA+AGG - chr4_3:64875525-64875544 None:intergenic 45.0%
! CTTGGCACCTTTTGCTTGTT+TGG + chr4_3:64875523-64875542 Msa0655910:CDS 45.0%
CTGCAAGCCAAACAAGCAAA+AGG - chr4_3:64875533-64875552 None:intergenic 45.0%
!!! CTTTTGCTTGTTTGGCTTGC+AGG + chr4_3:64875531-64875550 Msa0655910:CDS 45.0%
! TCGCTACATCTTTTGGACAG+TGG - chr4_3:64875701-64875720 None:intergenic 45.0%
CTGTCCAAAAGATGTAGCGA+TGG + chr4_3:64875701-64875720 Msa0655910:intron 45.0%
! CAACCCATCGCTACATCTTT+TGG - chr4_3:64875708-64875727 None:intergenic 45.0%
CAAAAGATGTAGCGATGGGT+TGG + chr4_3:64875706-64875725 Msa0655910:intron 45.0%
AAGACAATCAAGGGCTGCAA+TGG + chr4_3:64875902-64875921 Msa0655910:CDS 45.0%
GCAATTTGTGCTGCACTAAC+AGG + chr4_3:64875966-64875985 Msa0655910:CDS 45.0%
TGCTGCACTAACAGGTTTGA+TGG + chr4_3:64875974-64875993 Msa0655910:CDS 45.0%
CACTAACAGGTTTGATGGAG+AGG + chr4_3:64875979-64875998 Msa0655910:CDS 45.0%
TGATGAGACGGGATTCTCTT+TGG - chr4_3:64876031-64876050 None:intergenic 45.0%
GTGAAAGCTGCAATGGAGAA+TGG - chr4_3:64876577-64876596 None:intergenic 45.0%
GGATCAAGTGAAAGCTGCAA+TGG - chr4_3:64876584-64876603 None:intergenic 45.0%
GCAGTGGAAAGTTTATGAGG+AGG - chr4_3:64876633-64876652 None:intergenic 45.0%
GATAGGGTTCTTCAGATCAG+CGG - chr4_3:64876712-64876731 None:intergenic 45.0%
ACTGAAGAAGGAGGAAGTGA+AGG - chr4_3:64876749-64876768 None:intergenic 45.0%
! CTTGCGAGTGCAGAATTTTC+TGG + chr4_3:64876829-64876848 Msa0655910:three_prime_UTR 45.0%
!! GAGTGCAGAATTTTCTGGGA+AGG + chr4_3:64876834-64876853 Msa0655910:three_prime_UTR 45.0%
GAGGAAAGTGAATTGGGGAA+TGG + chr4_3:64876859-64876878 Msa0655910:three_prime_UTR 45.0%
GGGAATGGAAGATCCTTGAA+TGG + chr4_3:64876874-64876893 Msa0655910:three_prime_UTR 45.0%
! CGATGTTTGGGATCCATTCA+AGG - chr4_3:64876890-64876909 None:intergenic 45.0%
TGAATGGATCCCAAACATCG+AGG + chr4_3:64876890-64876909 Msa0655910:three_prime_UTR 45.0%
GAATGGATCCCAAACATCGA+GGG + chr4_3:64876891-64876910 Msa0655910:three_prime_UTR 45.0%
CTGATTCCAAGTTTCTTCGG+TGG - chr4_3:64876952-64876971 None:intergenic 45.0%
!!! ATAATTATATTTTATTTTGA+TGG + chr4_3:64877066-64877085 Msa0655910:three_prime_UTR 5.0%
TTGGTTGCTGCATTACCGTG+AGG + chr4_3:64874396-64874415 Msa0655910:CDS 50.0%
TGGTTGCTGCATTACCGTGA+GGG + chr4_3:64874397-64874416 Msa0655910:CDS 50.0%
TTAAGATAGGTGGTCCGACG+TGG + chr4_3:64874659-64874678 Msa0655910:intron 50.0%
CAAGGCCAATCCATGAATGG+AGG - chr4_3:64875507-64875526 None:intergenic 50.0%
!! CTCCATTCATGGATTGGCCT+TGG + chr4_3:64875505-64875524 Msa0655910:CDS 50.0%
TGCCAAGGCCAATCCATGAA+TGG - chr4_3:64875510-64875529 None:intergenic 50.0%
AGACGGGATTCTCTTTGGTG+TGG - chr4_3:64876026-64876045 None:intergenic 50.0%
GAATGATCAATGGCATCGCG+TGG - chr4_3:64876662-64876681 None:intergenic 50.0%
ACTTCCTCCTTCTTCAGTCC+TGG + chr4_3:64876751-64876770 Msa0655910:three_prime_UTR 50.0%
TCTTCCAGGACTGAAGAAGG+AGG - chr4_3:64876758-64876777 None:intergenic 50.0%
GGATCTTCCAGGACTGAAGA+AGG - chr4_3:64876761-64876780 None:intergenic 50.0%
TCCCAAACATCGAGGGAGAA+CGG + chr4_3:64876898-64876917 Msa0655910:three_prime_UTR 50.0%
TCCGTTCTCCCTCGATGTTT+GGG - chr4_3:64876902-64876921 None:intergenic 50.0%
ATCCGTTCTCCCTCGATGTT+TGG - chr4_3:64876903-64876922 None:intergenic 50.0%
TCGTCCGTCCAAGAAGTTGA+AGG - chr4_3:64876985-64877004 None:intergenic 50.0%
GGTTGCTGCATTACCGTGAG+GGG + chr4_3:64874398-64874417 Msa0655910:CDS 55.0%
CATGTGTGCCTGGTACTGTG+TGG - chr4_3:64875369-64875388 None:intergenic 55.0%
ATGGCTCCATGGCACATAGC+TGG + chr4_3:64875921-64875940 Msa0655910:CDS 55.0%
CTACCACCAGCTATGTGCCA+TGG - chr4_3:64875930-64875949 None:intergenic 55.0%
CCTCACTGTGACTGTGCCTA+AGG - chr4_3:64876551-64876570 None:intergenic 55.0%
CCTTAGGCACAGTCACAGTG+AGG + chr4_3:64876548-64876567 Msa0655910:three_prime_UTR 55.0%
GTGTTCAAGGCGGATCTTCC+AGG - chr4_3:64876772-64876791 None:intergenic 55.0%
! AGAAGCGCACGTGTTCAAGG+CGG - chr4_3:64876782-64876801 None:intergenic 55.0%
! TCCAGAAGCGCACGTGTTCA+AGG - chr4_3:64876785-64876804 None:intergenic 55.0%
!! AAGCACACGTGTGGACTGGA+AGG - chr4_3:64876812-64876831 None:intergenic 55.0%
CCAAGTTTCTTCGGTGGCAG+AGG - chr4_3:64876946-64876965 None:intergenic 55.0%
CCTCTGCCACCGAAGAAACT+TGG + chr4_3:64876943-64876962 Msa0655910:three_prime_UTR 55.0%
CAAGGGCTGCAATGGCTCCA+TGG + chr4_3:64875910-64875929 Msa0655910:CDS 60.0%
GCTCCATGGCACATAGCTGG+TGG + chr4_3:64875924-64875943 Msa0655910:CDS 60.0%
GCCTTGAACACGTGCGCTTC+TGG + chr4_3:64876781-64876800 Msa0655910:three_prime_UTR 60.0%
TCGCAAGCACACGTGTGGAC+TGG - chr4_3:64876816-64876835 None:intergenic 60.0%
TGCACTCGCAAGCACACGTG+TGG - chr4_3:64876821-64876840 None:intergenic 60.0%
TCTTCGGTGGCAGAGGCAGA+AGG - chr4_3:64876939-64876958 None:intergenic 60.0%
CATCGCGTGGAGCGTAGCAG+TGG - chr4_3:64876649-64876668 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 64874232 64877194 64874232 ID=Msa0655910;Name=Msa0655910
chr4_3 mRNA 64874232 64877194 64874232 ID=Msa0655910-mRNA-1;Parent=Msa0655910;Name=Msa0655910-mRNA-1;_AED=0.05;_eAED=0.05;_QI=96|1|1|1|1|1|4|1064|214
chr4_3 exon 64874232 64874459 64874232 ID=Msa0655910-mRNA-1:exon:18343;Parent=Msa0655910-mRNA-1
chr4_3 exon 64874587 64874635 64874587 ID=Msa0655910-mRNA-1:exon:18344;Parent=Msa0655910-mRNA-1
chr4_3 exon 64875353 64875552 64875353 ID=Msa0655910-mRNA-1:exon:18345;Parent=Msa0655910-mRNA-1
chr4_3 exon 64875867 64877194 64875867 ID=Msa0655910-mRNA-1:exon:18346;Parent=Msa0655910-mRNA-1
chr4_3 five_prime_UTR 64874232 64874327 64874232 ID=Msa0655910-mRNA-1:five_prime_utr;Parent=Msa0655910-mRNA-1
chr4_3 CDS 64874328 64874459 64874328 ID=Msa0655910-mRNA-1:cds;Parent=Msa0655910-mRNA-1
chr4_3 CDS 64874587 64874635 64874587 ID=Msa0655910-mRNA-1:cds;Parent=Msa0655910-mRNA-1
chr4_3 CDS 64875353 64875552 64875353 ID=Msa0655910-mRNA-1:cds;Parent=Msa0655910-mRNA-1
chr4_3 CDS 64875867 64876130 64875867 ID=Msa0655910-mRNA-1:cds;Parent=Msa0655910-mRNA-1
chr4_3 three_prime_UTR 64876131 64877194 64876131 ID=Msa0655910-mRNA-1:three_prime_utr;Parent=Msa0655910-mRNA-1
Gene Sequence

>Msa0655910

ATGCGTTCTTATAGTACCTCAATTTTGGCTCACTTGTTTGGCATAATTGCCCTCATCCTTATGCTTGTTTGGTTGCTGCATTACCGTGAGGGGATTGAATATGACTCTGGAAATCCACTTCGTGTTTTCAATGTTCACCCTTTTCTGATGTTCTTTGGGTTCATTTTCCTTGTTGGTCAAGCAATAATGTCTTACCACACAGTACCAGGCACACATGAAACACAGAAGATTGTTCACATGACACTTCATTTCATTGCCATAGTTCTTGGAATTGTTGGTATATGTGCCGTTTTCAAGTTCCATGATATGGTGAACTTGGTTGATGTTTACAGCCTCCATTCATGGATTGGCCTTGGCACCTTTTGCTTGTTTGGCTTGCAGTGGCTTTTTGGACTGACATTCATGTTTCAAGGAACAAGACAATCAAGGGCTGCAATGGCTCCATGGCACATAGCTGGTGGTAGAGCACTATTTTTCATGGCAATTTGTGCTGCACTAACAGGTTTGATGGAGAGGTCTGCTATGTTAAAACTAATGCCACACCAAAGAGAATCCCGTCTCATCAATTTTACTGGTCTAGCAATTCTCTTGTTTGGAGTTTTTGTGGATATGACAGTTGGTTTGGCCCATATTGTTTTTTCTTGA
Protein sequence

>Msa0655910

MRSYSTSILAHLFGIIALILMLVWLLHYREGIEYDSGNPLRVFNVHPFLMFFGFIFLVGQAIMSYHTVPGTHETQKIVHMTLHFIAIVLGIVGICAVFKFHDMVNLVDVYSLHSWIGLGTFCLFGLQWLFGLTFMFQGTRQSRAAMAPWHIAGGRALFFMAICAALTGLMERSAMLKLMPHQRESRLINFTGLAILLFGVFVDMTVGLAHIVFS*