AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1429860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429860 MtrunA17_Chr3g0137791 96.973 826 25 0 1 826 1 826 0.0 1680
Msa1429860 MtrunA17_Chr3g0137821 71.053 836 218 12 1 826 1 822 0.0 1213
Msa1429860 MtrunA17_Chr4g0003411 63.942 832 261 10 5 826 6 808 0.0 1080
Msa1429860 MtrunA17_Chr2g0333021 64.593 836 252 11 4 826 5 809 0.0 1076
Msa1429860 MtrunA17_Chr7g0246481 45.574 836 402 18 4 815 298 1104 0.0 715
Msa1429860 MtrunA17_Chr8g0350951 45.173 839 406 19 4 815 228 1039 0.0 711
Msa1429860 MtrunA17_Chr8g0380631 43.836 876 399 21 4 826 14 849 0.0 686
Msa1429860 MtrunA17_Chr3g0143471 43.068 880 417 21 5 826 13 866 0.0 677
Msa1429860 MtrunA17_Chr5g0398161 43.690 840 413 21 4 815 37 844 0.0 670
Msa1429860 MtrunA17_Chr5g0406161 40.365 877 448 17 5 826 16 872 0.0 660
Msa1429860 MtrunA17_Chr5g0398171 43.046 834 421 19 4 815 37 838 0.0 658
Msa1429860 MtrunA17_Chr2g0330431 44.156 770 380 17 62 826 32 756 0.0 621
Msa1429860 MtrunA17_Chr8g0341931 42.259 788 396 21 44 826 20 753 0.0 577
Msa1429860 MtrunA17_Chr1g0192401 31.727 249 133 11 511 749 733 954 1.33e-21 100
Msa1429860 MtrunA17_Chr1g0192401 31.064 235 119 9 217 438 355 559 5.35e-18 89.4
Msa1429860 MtrunA17_Chr2g0295411 32.836 201 103 6 511 706 783 956 2.27e-19 94.0
Msa1429860 MtrunA17_Chr2g0295411 28.627 255 127 10 217 449 381 602 1.06e-15 82.0
Msa1429860 MtrunA17_Chr5g0406151 50.000 68 30 1 335 402 16 79 2.15e-18 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429860 AT3G15730.1 62.530 838 269 10 3 826 4 810 0.0 1071
Msa1429860 AT1G52570.1 61.722 836 277 12 4 826 5 810 0.0 1043
Msa1429860 AT1G52570.3 61.722 836 277 12 4 826 5 810 0.0 1043
Msa1429860 AT1G52570.2 61.722 836 277 12 4 826 5 810 0.0 1043
Msa1429860 AT5G25370.1 61.594 828 300 12 4 826 6 820 0.0 1015
Msa1429860 AT5G25370.3 61.594 828 300 12 4 826 6 820 0.0 1015
Msa1429860 AT5G25370.2 61.594 828 300 12 4 826 6 820 0.0 1015
Msa1429860 AT5G25370.4 63.588 747 260 9 85 826 6 745 0.0 960
Msa1429860 AT4G11830.2 44.418 842 416 20 1 815 30 846 0.0 709
Msa1429860 AT4G11830.1 46.332 777 373 18 66 815 55 814 0.0 700
Msa1429860 AT2G42010.1 43.870 832 419 17 4 815 270 1073 0.0 698
Msa1429860 AT4G11840.1 43.839 844 416 19 2 815 41 856 0.0 693
Msa1429860 AT2G42010.2 43.174 857 414 19 4 815 270 1098 0.0 690
Msa1429860 AT4G11850.1 44.286 840 414 19 2 815 37 848 0.0 685
Msa1429860 AT4G00240.1 43.990 832 418 17 4 815 114 917 0.0 684
Msa1429860 AT4G11840.2 43.452 840 395 18 2 815 41 826 0.0 681
Msa1429860 AT4G00240.3 45.714 770 383 14 66 815 11 765 0.0 679
Msa1429860 AT4G00240.2 45.714 770 383 14 66 815 128 882 0.0 678
Msa1429860 AT4G35790.2 41.935 868 438 15 4 826 11 857 0.0 673
Msa1429860 AT4G35790.1 41.638 879 436 16 4 826 11 868 0.0 666
Msa1429860 AT1G55180.2 42.351 791 389 22 49 826 4 740 0.0 612
Msa1429860 AT1G55180.1 42.132 788 390 21 50 826 30 762 0.0 604
Msa1429860 AT4G11830.4 43.291 626 318 13 1 610 30 634 2.59e-169 504
Msa1429860 AT4G35790.3 40.294 680 352 10 4 640 11 679 1.11e-168 504
Msa1429860 AT4G11830.3 43.514 609 307 13 1 593 30 617 5.00e-165 493
Msa1429860 AT3G05630.1 28.099 726 352 31 143 771 304 956 5.15e-44 172
Msa1429860 AT3G16785.3 35.961 203 96 7 510 706 756 930 7.31e-22 102
Msa1429860 AT3G16785.3 31.250 224 102 8 232 438 389 577 4.80e-18 90.1
Msa1429860 AT3G16785.2 35.961 203 96 7 510 706 756 930 7.31e-22 102
Msa1429860 AT3G16785.2 31.250 224 102 8 232 438 389 577 4.80e-18 90.1
Msa1429860 AT3G16785.6 35.961 203 96 7 510 706 756 930 7.92e-22 102
Msa1429860 AT3G16785.6 31.250 224 102 8 232 438 389 577 4.33e-18 90.1
Msa1429860 AT3G16785.5 35.961 203 96 7 510 706 756 930 7.92e-22 102
Msa1429860 AT3G16785.5 31.250 224 102 8 232 438 389 577 4.33e-18 90.1
Msa1429860 AT3G16785.1 35.961 203 96 7 510 706 756 930 8.50e-22 102
Msa1429860 AT3G16785.1 31.250 224 102 8 232 438 389 577 4.58e-18 90.1
Msa1429860 AT3G16785.4 35.961 203 96 7 510 706 756 930 9.32e-22 102
Msa1429860 AT3G16785.4 31.250 224 102 8 232 438 389 577 4.29e-18 90.1

Find 173 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAATCACCTGGGAAATT+TGG 0.100944 tig0026572:-48189 None:intergenic
TTAATGCAATTCAAACTTTC+TGG 0.122895 tig0026572:-49661 None:intergenic
TCCCTTACCTATCATCATTT+TGG 0.178826 tig0026572:-49204 None:intergenic
TTGTATTTGAACATGGATTT+TGG 0.185371 tig0026572:-47446 None:intergenic
GCTTGAACTGAAGCACTTTC+AGG 0.203277 tig0026572:-48954 None:intergenic
GAAAGGTTTCATTCCATTTC+GGG 0.221154 tig0026572:-47246 None:intergenic
CCCATTGGAGCGACTCTAAT+TGG 0.224022 tig0026572:+47325 Msa1429860:CDS
AGCAGTTATGGTTCCATTAT+TGG 0.224317 tig0026572:-49788 None:intergenic
AATCCATATATTTGTCCTTT+TGG 0.234668 tig0026572:-49577 None:intergenic
ATCGATGCTGGTGCTGCTTC+TGG 0.242139 tig0026572:+48627 Msa1429860:CDS
GTCTATTACTGGTGGCTATA+TGG 0.261291 tig0026572:-49552 None:intergenic
CATCTTGGTAAAGAGTAATT+TGG 0.273836 tig0026572:-47567 None:intergenic
AAAACATTTCACAGATTATT+GGG 0.279095 tig0026572:+47158 Msa1429860:intron
TGAAAGGTTTCATTCCATTT+CGG 0.287963 tig0026572:-47247 None:intergenic
TTAATGTTCTTATGCTTGTT+TGG 0.298166 tig0026572:+47821 Msa1429860:CDS
CCAACAGAAGCCGCTGAAAA+AGG 0.298342 tig0026572:+48663 Msa1429860:CDS
CCATTATTGGTTACTTGTAT+TGG 0.306443 tig0026572:-49775 None:intergenic
CCAATACAAGTAACCAATAA+TGG 0.313741 tig0026572:+49775 Msa1429860:CDS
GATACTAAGGCTAAGATTCT+AGG 0.315033 tig0026572:+49835 Msa1429860:CDS
TGTCAACAATTATAGTCTTC+TGG 0.324805 tig0026572:-48020 None:intergenic
AAGAACATTAACACCTTCAT+TGG 0.328996 tig0026572:-47809 None:intergenic
ACATTCTTTAAACAAAATAA+TGG 0.329312 tig0026572:+47541 Msa1429860:CDS
GTTGTTCTTGTTGACTTGTT+TGG 0.332002 tig0026572:-47754 None:intergenic
TGGAGGAAGGTACTCAGATT+TGG 0.345102 tig0026572:-49860 None:intergenic
GGAACCATAACTGCTCTTCC+AGG 0.349450 tig0026572:+49796 Msa1429860:CDS
CCACCAAAAGGACAAATATA+TGG 0.350161 tig0026572:+49574 Msa1429860:CDS
CCCTTTATGACTTGTTCAAC+TGG 0.351702 tig0026572:-47361 None:intergenic
GTTGGAAGAACTAGAATGAT+AGG 0.353330 tig0026572:+47220 Msa1429860:CDS
TAATGTTCTTATGCTTGTTT+GGG 0.358547 tig0026572:+47822 Msa1429860:CDS
ACTCCATCCAATGGAATATA+CGG 0.365105 tig0026572:-47613 None:intergenic
GATATTAAAGTTGAGGATAT+TGG 0.365230 tig0026572:+48822 Msa1429860:CDS
TCCAGTTGAACAAGTCATAA+AGG 0.366172 tig0026572:+47360 Msa1429860:CDS
ATATAATCACTGTCAGGTTC+TGG 0.366661 tig0026572:-49140 None:intergenic
CTTTAAGAAAGATGGCTTGA+TGG 0.371228 tig0026572:+47867 Msa1429860:CDS
CAGTTCAAGCAATATTAGAT+TGG 0.371630 tig0026572:+48967 Msa1429860:CDS
TTTAGTCCTTGTGACCAGTT+TGG 0.378654 tig0026572:-47490 None:intergenic
GTTCCTGACTTTAAGAAAGA+TGG 0.382115 tig0026572:+47859 Msa1429860:CDS
CATGGCTAGGTGGTGAGAAT+TGG 0.390572 tig0026572:-49880 None:intergenic
TAAAATGACAAGTAATCTCT+TGG 0.395875 tig0026572:-49062 None:intergenic
AGAGTACAATTACATGGAAA+TGG 0.400127 tig0026572:-46486 None:intergenic
TTACTGGTGGCTATATGGTT+TGG 0.403321 tig0026572:-49547 None:intergenic
CAAGATGCACATGTTCTAGA+TGG 0.408428 tig0026572:+47583 Msa1429860:CDS
GATGATGAATACATAATCAT+TGG 0.412861 tig0026572:+49454 Msa1429860:CDS
TATGAGCACCTTGGAGATAT+TGG 0.417376 tig0026572:+49616 Msa1429860:CDS
GTTCATGCCAAAATGATGAT+AGG 0.420607 tig0026572:+49197 Msa1429860:CDS
AGAATCTTAGCCTTAGTATC+AGG 0.421693 tig0026572:-49832 None:intergenic
TTACATGGAAATGGAGGTTA+AGG 0.423456 tig0026572:-46477 None:intergenic
CAAGAACAACCATCACTCTT+GGG 0.423882 tig0026572:+47767 Msa1429860:CDS
CGGCTTCTGTTGGATCTTGA+GGG 0.427130 tig0026572:-48653 None:intergenic
AAATCCTGGAAGAGCAGTTA+TGG 0.432056 tig0026572:-49800 None:intergenic
GCACTTTCAGGTATACCTTC+TGG 0.435666 tig0026572:-48942 None:intergenic
ATCTTCAGTGACAACTCCTT+TGG 0.436891 tig0026572:-48861 None:intergenic
GCGGCTTCTGTTGGATCTTG+AGG 0.438003 tig0026572:-48654 None:intergenic
AAGCCATCTTTCTTAAAGTC+AGG 0.443766 tig0026572:-47862 None:intergenic
AGATTGGCAAAGAAGGACTA+TGG 0.445851 tig0026572:+48983 Msa1429860:CDS
CAATATTAGATTGGCAAAGA+AGG 0.446472 tig0026572:+48976 Msa1429860:CDS
GAATGATAGGTAGCCCGAAA+TGG 0.447072 tig0026572:+47233 Msa1429860:CDS
CCAATTAGAGTCGCTCCAAT+GGG 0.447711 tig0026572:-47325 None:intergenic
GATATTCATTGTAAACTAGA+AGG 0.448111 tig0026572:+48432 Msa1429860:CDS
GTTCCGTATATTCCATTGGA+TGG 0.448817 tig0026572:+47610 Msa1429860:CDS
TTCTAGGTAGTTCATATGGT+TGG 0.454363 tig0026572:+48790 Msa1429860:CDS
GATACACTACAAACTCTTGC+CGG 0.455373 tig0026572:+46669 Msa1429860:CDS
CTTGTTTGGATCTCTAATCA+AGG 0.457534 tig0026572:-47740 None:intergenic
GTGACAACTCCTTTGGTATG+AGG 0.458272 tig0026572:-48854 None:intergenic
CTTTGAGTATATGTCCCAAT+TGG 0.459345 tig0026572:-49707 None:intergenic
ACAAGAACAACCATCACTCT+TGG 0.459719 tig0026572:+47766 Msa1429860:CDS
CCAGTTGAACAAGTCATAAA+GGG 0.460625 tig0026572:+47361 Msa1429860:CDS
CACAACACATCCCATGCAAC+AGG 0.462297 tig0026572:-48456 None:intergenic
CGAAACATGCTTGAAGGTAG+TGG 0.463688 tig0026572:-48525 None:intergenic
AGAGACAGTGAGATTGCAAT+AGG 0.465168 tig0026572:+49514 Msa1429860:CDS
TGAGGAACTCCTTGAAATCC+TGG 0.466088 tig0026572:-47517 None:intergenic
ATATCATGCCATGGCTCTCT+AGG 0.466709 tig0026572:-48414 None:intergenic
TTCTGTTCCGTATATTCCAT+TGG 0.467036 tig0026572:+47606 Msa1429860:CDS
ATTGTTAGCAAGATTGAAGC+TGG 0.474341 tig0026572:+48882 Msa1429860:CDS
CTAAAGAGTCCAGGATTTCA+AGG 0.476603 tig0026572:+47508 Msa1429860:CDS
CATTGATCTTTGATTTATGT+TGG 0.476676 tig0026572:-49482 None:intergenic
TCAGGAAAACATTCAAATCC+TGG 0.477356 tig0026572:-49814 None:intergenic
GTTGAAGCCCTTCAAAGAAA+AGG 0.478206 tig0026572:+49026 Msa1429860:CDS
CAGTTTCTCTCCACATGGCT+AGG 0.479672 tig0026572:-49893 None:intergenic
TCAGGTTCTGGTGCCTCTGT+AGG 0.480853 tig0026572:-49128 None:intergenic
AACTGGTTGGTCTGTTTACA+CGG 0.480935 tig0026572:+47711 Msa1429860:CDS
CATCTAGAACATGTGCATCT+TGG 0.481308 tig0026572:-47582 None:intergenic
ATGGATTTAGACGTGCACTA+TGG 0.481715 tig0026572:+49593 Msa1429860:CDS
CATTTCACAGATTATTGGGA+TGG 0.486910 tig0026572:+47162 Msa1429860:intron
TCCAATTAGAGTCGCTCCAA+TGG 0.488605 tig0026572:-47326 None:intergenic
GATTTAGACAAAGCAAGAGT+TGG 0.492961 tig0026572:+47202 Msa1429860:CDS
GGCTAGGTGGTGAGAATTGG+AGG 0.496635 tig0026572:-49877 None:intergenic
AAAATGACAAGTAATCTCTT+GGG 0.497438 tig0026572:-49061 None:intergenic
TATTCACGAAACATGCTTGA+AGG 0.498456 tig0026572:-48531 None:intergenic
GTATGTGCCGGGAAAATGTT+GGG 0.500103 tig0026572:+47642 Msa1429860:CDS
TTGTTTAGATCGATCGATGC+TGG 0.502376 tig0026572:+48615 Msa1429860:CDS
GATGGAAGAAGTATAGTTCA+AGG 0.503461 tig0026572:+47967 Msa1429860:CDS
GTGGGATTGATCTTTGTGAT+GGG 0.506802 tig0026572:+48097 Msa1429860:CDS
CATGGAAATGGAGGTTAAGG+AGG 0.508674 tig0026572:-46474 None:intergenic
AGGGCAACATAGTGAATACA+TGG 0.511698 tig0026572:+47380 Msa1429860:CDS
GCTCTAATATAATCACTGTC+AGG 0.514466 tig0026572:-49146 None:intergenic
GCTTGATGTGACTATATATG+AGG 0.515858 tig0026572:+46644 Msa1429860:CDS
TTTGACAGTGAATATAATGT+TGG 0.517344 tig0026572:-47293 None:intergenic
TGGAGTAGAACGGTATGTGC+CGG 0.519752 tig0026572:+47630 Msa1429860:CDS
CAACATAAATCAAAGATCAA+TGG 0.522850 tig0026572:+49483 Msa1429860:CDS
AACTAGAAGGACCTGTTGCA+TGG 0.522952 tig0026572:+48445 Msa1429860:CDS
GGAGTAGAACGGTATGTGCC+GGG 0.525843 tig0026572:+47631 Msa1429860:CDS
GGTGGGATTGATCTTTGTGA+TGG 0.527896 tig0026572:+48096 Msa1429860:CDS
TTTCATCAACCAAATTTCCC+AGG 0.530381 tig0026572:+48180 Msa1429860:CDS
GGTATACCTTCTGGCCACAT+TGG 0.533593 tig0026572:-48933 None:intergenic
CGGCAAATCAAAACAACACA+TGG 0.534970 tig0026572:+48583 Msa1429860:CDS
CTTAAGAAGAAAGCCAATGA+AGG 0.539673 tig0026572:+47796 Msa1429860:CDS
TCTTACCCTCTCGGTTTCCA+AGG 0.540213 tig0026572:-49088 None:intergenic
CAAAGATGGGAAAAGCAAGT+TGG 0.540379 tig0026572:+48489 Msa1429860:CDS
GATATACCTCTGGCTTGCAT+TGG 0.543798 tig0026572:-47014 None:intergenic
ACTATACTTCTTCCATCACC+AGG 0.545798 tig0026572:-47961 None:intergenic
GCAGAGATCGAAATTGCATC+CGG 0.546902 tig0026572:-46688 None:intergenic
CTTCCATCACCAGGGTTACG+TGG 0.548889 tig0026572:-47952 None:intergenic
TTGATAGAATTAATCAGCAA+TGG 0.551114 tig0026572:-49940 None:intergenic
ATTTCACAGATTATTGGGAT+GGG 0.555183 tig0026572:+47163 Msa1429860:intron
ACTAGAAGGACCTGTTGCAT+GGG 0.560087 tig0026572:+48446 Msa1429860:CDS
GTCATGTTGCACTTACTACA+CGG 0.562058 tig0026572:+46618 Msa1429860:exon
GTGGATCATCGTCCAATACA+AGG 0.562412 tig0026572:+47418 Msa1429860:CDS
AAGGGAACATGCACAAGCGT+GGG 0.570770 tig0026572:+46946 Msa1429860:intron
TTCATATGGTTGGAAATCAT+CGG 0.572489 tig0026572:+48800 Msa1429860:CDS
ACCAGTAATAGACCACCAAA+AGG 0.573701 tig0026572:+49562 Msa1429860:CDS
ATGGGTTAATATATTAGATG+TGG 0.574499 tig0026572:+47399 Msa1429860:CDS
GTCATTCCAATGTGGCCAGA+AGG 0.574690 tig0026572:+48927 Msa1429860:CDS
TACCGTTCTACTCCATCCAA+TGG 0.576822 tig0026572:-47622 None:intergenic
ATGGAAATGGAGGTTAAGGA+GGG 0.580560 tig0026572:-46473 None:intergenic
TGCTTTGCACCTCATACCAA+AGG 0.582854 tig0026572:+48845 Msa1429860:CDS
TGACAAGTAATCTCTTGGGT+TGG 0.586202 tig0026572:-49057 None:intergenic
GTCAAACAAGATAACCCCAT+TGG 0.587028 tig0026572:+47310 Msa1429860:CDS
ATCATCGGATATTAAAGTTG+AGG 0.587336 tig0026572:+48815 Msa1429860:CDS
AAGAAGCTCCCCAAGAGTGA+TGG 0.594868 tig0026572:-47776 None:intergenic
TCACAAGGACTAAAGAGTCC+AGG 0.595640 tig0026572:+47499 Msa1429860:CDS
TCGTCGAATAGCGTTTATGT+AGG 0.595975 tig0026572:-48733 None:intergenic
CAAGGGAACATGCACAAGCG+TGG 0.596680 tig0026572:+46945 Msa1429860:intron
CAATTTCGATCTCTGCAACA+AGG 0.597917 tig0026572:+46695 Msa1429860:CDS
TGAAAACAAAGGATGTTCCA+TGG 0.602384 tig0026572:-48130 None:intergenic
TATTGCCTTGGAAACCGAGA+GGG 0.604275 tig0026572:+49083 Msa1429860:CDS
TGCCCACGTAACCCTGGTGA+TGG 0.604924 tig0026572:+47949 Msa1429860:CDS
GGTATGTGCCGGGAAAATGT+TGG 0.606197 tig0026572:+47641 Msa1429860:CDS
TTTCTCTCCACATGGCTAGG+TGG 0.613613 tig0026572:-49890 None:intergenic
GTGAGAATTGTATTTGAACA+TGG 0.616949 tig0026572:-47453 None:intergenic
TTACAATGAATATCATGCCA+TGG 0.618761 tig0026572:-48423 None:intergenic
ACATTCAGTTTCTCTCCACA+TGG 0.623036 tig0026572:-49898 None:intergenic
ATGTGCAGAGTAAATGTGAA+AGG 0.624116 tig0026572:-47263 None:intergenic
ATAAATCAAAGATCAATGGA+TGG 0.626562 tig0026572:+49487 Msa1429860:CDS
TTCCATCACCAGGGTTACGT+GGG 0.629779 tig0026572:-47951 None:intergenic
GTACAATTACATGGAAATGG+AGG 0.633738 tig0026572:-46483 None:intergenic
AAGAACAACCATCACTCTTG+GGG 0.634263 tig0026572:+47768 Msa1429860:CDS
GTATTCAAAGTTGAAAACAA+AGG 0.635550 tig0026572:-48141 None:intergenic
GCACTATGGTATGAGCACCT+TGG 0.638088 tig0026572:+49607 Msa1429860:CDS
AATGATCCAAACTGGTCACA+AGG 0.638447 tig0026572:+47484 Msa1429860:CDS
CAATTGGGACATATACTCAA+AGG 0.640794 tig0026572:+49708 Msa1429860:CDS
AGGTGGTGAGAATTGGAGGA+AGG 0.643329 tig0026572:-49873 None:intergenic
GAATGAATATACTCCTACAG+AGG 0.643986 tig0026572:+49115 Msa1429860:CDS
TCATGTTGCACTTACTACAC+GGG 0.647685 tig0026572:+46619 Msa1429860:exon
TTGTATCACCAATATCTCCA+AGG 0.650254 tig0026572:-49624 None:intergenic
CTTGTATTGGATAATGCATG+AGG 0.661800 tig0026572:-49762 None:intergenic
TCGTGTATCGAATGTTGCCA+CGG 0.661868 tig0026572:+48563 Msa1429860:CDS
AAAGATTCCTGCTTAAAACA+AGG 0.666302 tig0026572:-49969 None:intergenic
ATTCTCACCACCTAGCCATG+TGG 0.671398 tig0026572:+49883 Msa1429860:exon
TTCCATTGGATGGAGTAGAA+CGG 0.671871 tig0026572:+47620 Msa1429860:CDS
GGTGCTTCTATTAACAAAGG+TGG 0.672280 tig0026572:+48390 Msa1429860:intron
ACAGGTGCTTCTATTAACAA+AGG 0.672622 tig0026572:+48387 Msa1429860:intron
AAGTTGCTAGTTCTGCACAA+TGG 0.673752 tig0026572:+48046 Msa1429860:CDS
AGGGAACATGCACAAGCGTG+GGG 0.679420 tig0026572:+46947 Msa1429860:intron
TGATGGGAGATATGATACCA+TGG 0.695563 tig0026572:+48113 Msa1429860:CDS
CTATACTTCTTCCATCACCA+GGG 0.698859 tig0026572:-47960 None:intergenic
AAGGTGGTCCTAGAGAGCCA+TGG 0.705923 tig0026572:+48406 Msa1429860:CDS
GGGCAACATAGTGAATACAT+GGG 0.707757 tig0026572:+47381 Msa1429860:CDS
TCAATGCCAATGCAAGCCAG+AGG 0.708442 tig0026572:+47008 Msa1429860:CDS
CAATTAGAGTCGCTCCAATG+GGG 0.713373 tig0026572:-47324 None:intergenic
TTATTGCCTTGGAAACCGAG+AGG 0.718876 tig0026572:+49082 Msa1429860:CDS
TGTACATTGTCATTCCAATG+TGG 0.747438 tig0026572:+48919 Msa1429860:CDS
GATCATCGTCCAATACAAGG+CGG 0.779275 tig0026572:+47421 Msa1429860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTCTTTAAACAAAATAA+TGG + tig0026572:47541-47560 Msa1429860:CDS 15.0%
!! AAAATATAAAATATTCCAAC+AGG + tig0026572:48369-48388 Msa1429860:intron 15.0%
!! AATAAATGTAAAATAGAAAC+AGG - tig0026572:49246-49265 None:intergenic 15.0%
!!! TAATAAATTTTCTTTTGCAA+AGG - tig0026572:49387-49406 None:intergenic 15.0%
!! CAATCTACTTAAAATGAAAT+TGG - tig0026572:46758-46777 None:intergenic 20.0%
!! TGACAAATTCATTAGAAAAA+AGG - tig0026572:46825-46844 None:intergenic 20.0%
!! AAAAACATTTCACAGATTAT+TGG + tig0026572:47157-47176 Msa1429860:intron 20.0%
!! AAAACATTTCACAGATTATT+GGG + tig0026572:47158-47177 Msa1429860:intron 20.0%
!!! ATTGAAAATCAGTATTTTCT+AGG + tig0026572:48774-48793 Msa1429860:CDS 20.0%
!!! TTGAACTTCAAATTTTATAG+AGG - tig0026572:50017-50036 None:intergenic 20.0%
!! TAAGTAAGAGTACAATTACA+TGG - tig0026572:46495-46514 None:intergenic 25.0%
! CTTTCAAATTTCGTAAACAA+GGG + tig0026572:46928-46947 Msa1429860:intron 25.0%
! TACATGTTATATAGTCAACA+AGG - tig0026572:47059-47078 None:intergenic 25.0%
! TAGGTGTTAGAATGTATAAA+GGG - tig0026572:47129-47148 None:intergenic 25.0%
! TTAGGTGTTAGAATGTATAA+AGG - tig0026572:47130-47149 None:intergenic 25.0%
!!! GAAATGTTTTTACATGTGTT+AGG - tig0026572:47148-47167 None:intergenic 25.0%
! TTTGACAGTGAATATAATGT+TGG - tig0026572:47296-47315 None:intergenic 25.0%
!! ATGGGTTAATATATTAGATG+TGG + tig0026572:47399-47418 Msa1429860:CDS 25.0%
!! TTGTATTTGAACATGGATTT+TGG - tig0026572:47449-47468 None:intergenic 25.0%
! ATGTTAAAAATGATCCAAAC+TGG + tig0026572:47476-47495 Msa1429860:CDS 25.0%
!!! TTTTGTTTAAAGAATGTGTG+AGG - tig0026572:47538-47557 None:intergenic 25.0%
!!! AAGCATTTTATTTGCATAAC+TGG + tig0026572:47694-47713 Msa1429860:CDS 25.0%
!!! ATTTTATTTGCATAACTGGT+TGG + tig0026572:47698-47717 Msa1429860:CDS 25.0%
! TTAATGTTCTTATGCTTGTT+TGG + tig0026572:47821-47840 Msa1429860:CDS 25.0%
! TAATGTTCTTATGCTTGTTT+GGG + tig0026572:47822-47841 Msa1429860:CDS 25.0%
! GTATTCAAAGTTGAAAACAA+AGG - tig0026572:48144-48163 None:intergenic 25.0%
! AGGACTTAATTATGAAATGT+TGG + tig0026572:48326-48345 Msa1429860:intron 25.0%
! GATATTCATTGTAAACTAGA+AGG + tig0026572:48432-48451 Msa1429860:CDS 25.0%
!! TATTTTCTAGGTAGTTCATA+TGG + tig0026572:48786-48805 Msa1429860:CDS 25.0%
! GATATTAAAGTTGAGGATAT+TGG + tig0026572:48822-48841 Msa1429860:CDS 25.0%
! AAAATGACAAGTAATCTCTT+GGG - tig0026572:49064-49083 None:intergenic 25.0%
! TAAAATGACAAGTAATCTCT+TGG - tig0026572:49065-49084 None:intergenic 25.0%
! GAAACAGGTAATATATATGT+TGG - tig0026572:49231-49250 None:intergenic 25.0%
!!! ATTGGAAAGTGTCAATTTAA+TGG - tig0026572:49349-49368 None:intergenic 25.0%
! GATGATGAATACATAATCAT+TGG + tig0026572:49454-49473 Msa1429860:CDS 25.0%
!! CATTGATCTTTGATTTATGT+TGG - tig0026572:49485-49504 None:intergenic 25.0%
! CAACATAAATCAAAGATCAA+TGG + tig0026572:49483-49502 Msa1429860:CDS 25.0%
! ATAAATCAAAGATCAATGGA+TGG + tig0026572:49487-49506 Msa1429860:CDS 25.0%
!! AATCCATATATTTGTCCTTT+TGG - tig0026572:49580-49599 None:intergenic 25.0%
! TTAATGCAATTCAAACTTTC+TGG - tig0026572:49664-49683 None:intergenic 25.0%
! TTGATAGAATTAATCAGCAA+TGG - tig0026572:49943-49962 None:intergenic 25.0%
!! ACTTCAAATTTTATAGAGGT+AGG - tig0026572:50013-50032 None:intergenic 25.0%
AGAGTACAATTACATGGAAA+TGG - tig0026572:46489-46508 None:intergenic 30.0%
GCTTTCAAATTTCGTAAACA+AGG + tig0026572:46927-46946 Msa1429860:intron 30.0%
AATATGTGAAGATATACCTC+TGG - tig0026572:47027-47046 None:intergenic 30.0%
ATTTCACAGATTATTGGGAT+GGG + tig0026572:47163-47182 Msa1429860:intron 30.0%
TGAAAGGTTTCATTCCATTT+CGG - tig0026572:47250-47269 None:intergenic 30.0%
GTGAGAATTGTATTTGAACA+TGG - tig0026572:47456-47475 None:intergenic 30.0%
CATCTTGGTAAAGAGTAATT+TGG - tig0026572:47570-47589 None:intergenic 30.0%
AAGAACATTAACACCTTCAT+TGG - tig0026572:47812-47831 None:intergenic 30.0%
TGTCAACAATTATAGTCTTC+TGG - tig0026572:48023-48042 None:intergenic 30.0%
!! AGAACAATCACAAGTTTTGT+TGG + tig0026572:48075-48094 Msa1429860:CDS 30.0%
GCATGCATATTTACAATCTT+AGG + tig0026572:48306-48325 Msa1429860:intron 30.0%
TTACAATGAATATCATGCCA+TGG - tig0026572:48426-48445 None:intergenic 30.0%
TTCATATGGTTGGAAATCAT+CGG + tig0026572:48800-48819 Msa1429860:CDS 30.0%
ATCATCGGATATTAAAGTTG+AGG + tig0026572:48815-48834 Msa1429860:CDS 30.0%
CAGTTCAAGCAATATTAGAT+TGG + tig0026572:48967-48986 Msa1429860:CDS 30.0%
CAATATTAGATTGGCAAAGA+AGG + tig0026572:48976-48995 Msa1429860:CDS 30.0%
! TCTGATTCCTTTTCTTTGAA+GGG - tig0026572:49036-49055 None:intergenic 30.0%
!!! TACTTGTCATTTTATTGCCT+TGG + tig0026572:49071-49090 Msa1429860:CDS 30.0%
AGGTGTATGTGCTAATTAAT+TGG - tig0026572:49367-49386 None:intergenic 30.0%
! CCATTATTGGTTACTTGTAT+TGG - tig0026572:49778-49797 None:intergenic 30.0%
CCAATACAAGTAACCAATAA+TGG + tig0026572:49775-49794 Msa1429860:CDS 30.0%
! TGAATGTTTTCCTGATACTA+AGG + tig0026572:49822-49841 Msa1429860:CDS 30.0%
AAAGATTCCTGCTTAAAACA+AGG - tig0026572:49972-49991 None:intergenic 30.0%
TTACATGGAAATGGAGGTTA+AGG - tig0026572:46480-46499 None:intergenic 35.0%
GTACAATTACATGGAAATGG+AGG - tig0026572:46486-46505 None:intergenic 35.0%
!! TGTTTTTATTGCACACAGCA+AGG + tig0026572:46568-46587 Msa1429860:intron 35.0%
! GTTTTTATTGCACACAGCAA+GGG + tig0026572:46569-46588 Msa1429860:intron 35.0%
GCTTGATGTGACTATATATG+AGG + tig0026572:46644-46663 Msa1429860:CDS 35.0%
!! CTCTGTGTATTTGCTGATTT+CGG + tig0026572:46905-46924 Msa1429860:intron 35.0%
CATTTCACAGATTATTGGGA+TGG + tig0026572:47162-47181 Msa1429860:intron 35.0%
GATTTAGACAAAGCAAGAGT+TGG + tig0026572:47202-47221 Msa1429860:CDS 35.0%
GTTGGAAGAACTAGAATGAT+AGG + tig0026572:47220-47239 Msa1429860:CDS 35.0%
GAAAGGTTTCATTCCATTTC+GGG - tig0026572:47249-47268 None:intergenic 35.0%
ATGTGCAGAGTAAATGTGAA+AGG - tig0026572:47266-47285 None:intergenic 35.0%
TCCAGTTGAACAAGTCATAA+AGG + tig0026572:47360-47379 Msa1429860:CDS 35.0%
CCAGTTGAACAAGTCATAAA+GGG + tig0026572:47361-47380 Msa1429860:CDS 35.0%
TTCTGTTCCGTATATTCCAT+TGG + tig0026572:47606-47625 Msa1429860:CDS 35.0%
ACTCCATCCAATGGAATATA+CGG - tig0026572:47616-47635 None:intergenic 35.0%
CTTGTTTGGATCTCTAATCA+AGG - tig0026572:47743-47762 None:intergenic 35.0%
!! GTTGTTCTTGTTGACTTGTT+TGG - tig0026572:47757-47776 None:intergenic 35.0%
CTTAAGAAGAAAGCCAATGA+AGG + tig0026572:47796-47815 Msa1429860:CDS 35.0%
! GTTCCTGACTTTAAGAAAGA+TGG + tig0026572:47859-47878 Msa1429860:CDS 35.0%
AAGCCATCTTTCTTAAAGTC+AGG - tig0026572:47865-47884 None:intergenic 35.0%
! CTTTAAGAAAGATGGCTTGA+TGG + tig0026572:47867-47886 Msa1429860:CDS 35.0%
! GATGGAAGAAGTATAGTTCA+AGG + tig0026572:47967-47986 Msa1429860:CDS 35.0%
!!! ACAATCACAAGTTTTGTTGG+TGG + tig0026572:48078-48097 Msa1429860:CDS 35.0%
!!! CAATCACAAGTTTTGTTGGT+GGG + tig0026572:48079-48098 Msa1429860:CDS 35.0%
TGAAAACAAAGGATGTTCCA+TGG - tig0026572:48133-48152 None:intergenic 35.0%
TTTCATCAACCAAATTTCCC+AGG + tig0026572:48180-48199 Msa1429860:CDS 35.0%
AACTAGTTCCAAAATCACCT+GGG - tig0026572:48200-48219 None:intergenic 35.0%
AAACTAGTTCCAAAATCACC+TGG - tig0026572:48201-48220 None:intergenic 35.0%
!! TTGTTAATAGAAGCACCTGT+TGG - tig0026572:48387-48406 None:intergenic 35.0%
ACAGGTGCTTCTATTAACAA+AGG + tig0026572:48387-48406 Msa1429860:intron 35.0%
! TGTGCAATTTTGAGCAAAGA+TGG + tig0026572:48475-48494 Msa1429860:CDS 35.0%
! GTGCAATTTTGAGCAAAGAT+GGG + tig0026572:48476-48495 Msa1429860:CDS 35.0%
TATTCACGAAACATGCTTGA+AGG - tig0026572:48534-48553 None:intergenic 35.0%
!!! AGTAACAAGACCTTTTTCAG+CGG - tig0026572:48676-48695 None:intergenic 35.0%
TTCTAGGTAGTTCATATGGT+TGG + tig0026572:48790-48809 Msa1429860:CDS 35.0%
ATTGTTAGCAAGATTGAAGC+TGG + tig0026572:48882-48901 Msa1429860:CDS 35.0%
TGTACATTGTCATTCCAATG+TGG + tig0026572:48919-48938 Msa1429860:CDS 35.0%
! CTCTGATTCCTTTTCTTTGA+AGG - tig0026572:49037-49056 None:intergenic 35.0%
! CATTCATTTTCTTACCCTCT+CGG - tig0026572:49100-49119 None:intergenic 35.0%
GAATGAATATACTCCTACAG+AGG + tig0026572:49115-49134 Msa1429860:CDS 35.0%
ATATAATCACTGTCAGGTTC+TGG - tig0026572:49143-49162 None:intergenic 35.0%
GCTCTAATATAATCACTGTC+AGG - tig0026572:49149-49168 None:intergenic 35.0%
GTTCATGCCAAAATGATGAT+AGG + tig0026572:49197-49216 Msa1429860:CDS 35.0%
TGCCAAAATGATGATAGGTA+AGG + tig0026572:49202-49221 Msa1429860:intron 35.0%
GCCAAAATGATGATAGGTAA+GGG + tig0026572:49203-49222 Msa1429860:intron 35.0%
! TCCCTTACCTATCATCATTT+TGG - tig0026572:49207-49226 None:intergenic 35.0%
AACGCAGTTCAAATATCTTC+GGG - tig0026572:49299-49318 None:intergenic 35.0%
GAACGCAGTTCAAATATCTT+CGG - tig0026572:49300-49319 None:intergenic 35.0%
TATTTGAACTGCGTTCTTTG+AGG + tig0026572:49304-49323 Msa1429860:intron 35.0%
! CCATATATTTGTCCTTTTGG+TGG - tig0026572:49577-49596 None:intergenic 35.0%
CCACCAAAAGGACAAATATA+TGG + tig0026572:49574-49593 Msa1429860:CDS 35.0%
TTGTATCACCAATATCTCCA+AGG - tig0026572:49627-49646 None:intergenic 35.0%
CTTTGAGTATATGTCCCAAT+TGG - tig0026572:49710-49729 None:intergenic 35.0%
CAATTGGGACATATACTCAA+AGG + tig0026572:49708-49727 Msa1429860:CDS 35.0%
CTTGTATTGGATAATGCATG+AGG - tig0026572:49765-49784 None:intergenic 35.0%
AGCAGTTATGGTTCCATTAT+TGG - tig0026572:49791-49810 None:intergenic 35.0%
TCAGGAAAACATTCAAATCC+TGG - tig0026572:49817-49836 None:intergenic 35.0%
!! AGAATCTTAGCCTTAGTATC+AGG - tig0026572:49835-49854 None:intergenic 35.0%
GATACTAAGGCTAAGATTCT+AGG + tig0026572:49835-49854 Msa1429860:CDS 35.0%
!!! AGTAACACCTTGTTTTAAGC+AGG + tig0026572:49962-49981 Msa1429860:three_prime_UTR 35.0%
ATGGAAATGGAGGTTAAGGA+GGG - tig0026572:46476-46495 None:intergenic 40.0%
GTCATGTTGCACTTACTACA+CGG + tig0026572:46618-46637 Msa1429860:exon 40.0%
TCATGTTGCACTTACTACAC+GGG + tig0026572:46619-46638 Msa1429860:exon 40.0%
GATACACTACAAACTCTTGC+CGG + tig0026572:46669-46688 Msa1429860:CDS 40.0%
CAATTTCGATCTCTGCAACA+AGG + tig0026572:46695-46714 Msa1429860:CDS 40.0%
CGAAATCAGCAAATACACAG+AGG - tig0026572:46907-46926 None:intergenic 40.0%
GTCAAACAAGATAACCCCAT+TGG + tig0026572:47310-47329 Msa1429860:CDS 40.0%
!! CCCTTTATGACTTGTTCAAC+TGG - tig0026572:47364-47383 None:intergenic 40.0%
AGGGCAACATAGTGAATACA+TGG + tig0026572:47380-47399 Msa1429860:CDS 40.0%
GGGCAACATAGTGAATACAT+GGG + tig0026572:47381-47400 Msa1429860:CDS 40.0%
!!! ATTTTGGAACCGCCTTGTAT+TGG - tig0026572:47433-47452 None:intergenic 40.0%
AATGATCCAAACTGGTCACA+AGG + tig0026572:47484-47503 Msa1429860:CDS 40.0%
TTTAGTCCTTGTGACCAGTT+TGG - tig0026572:47493-47512 None:intergenic 40.0%
CTAAAGAGTCCAGGATTTCA+AGG + tig0026572:47508-47527 Msa1429860:CDS 40.0%
! CATCTAGAACATGTGCATCT+TGG - tig0026572:47585-47604 None:intergenic 40.0%
! CAAGATGCACATGTTCTAGA+TGG + tig0026572:47583-47602 Msa1429860:CDS 40.0%
GTTCCGTATATTCCATTGGA+TGG + tig0026572:47610-47629 Msa1429860:CDS 40.0%
TTCCATTGGATGGAGTAGAA+CGG + tig0026572:47620-47639 Msa1429860:CDS 40.0%
! GTATCTTCCCAACATTTTCC+CGG - tig0026572:47652-47671 None:intergenic 40.0%
!! AACTGGTTGGTCTGTTTACA+CGG + tig0026572:47711-47730 Msa1429860:CDS 40.0%
ACAAGAACAACCATCACTCT+TGG + tig0026572:47766-47785 Msa1429860:CDS 40.0%
CAAGAACAACCATCACTCTT+GGG + tig0026572:47767-47786 Msa1429860:CDS 40.0%
AAGAACAACCATCACTCTTG+GGG + tig0026572:47768-47787 Msa1429860:CDS 40.0%
CTATACTTCTTCCATCACCA+GGG - tig0026572:47963-47982 None:intergenic 40.0%
ACTATACTTCTTCCATCACC+AGG - tig0026572:47964-47983 None:intergenic 40.0%
!! AAGTTGCTAGTTCTGCACAA+TGG + tig0026572:48046-48065 Msa1429860:CDS 40.0%
GTGGGATTGATCTTTGTGAT+GGG + tig0026572:48097-48116 Msa1429860:CDS 40.0%
TGATGGGAGATATGATACCA+TGG + tig0026572:48113-48132 Msa1429860:CDS 40.0%
CCAAAATCACCTGGGAAATT+TGG - tig0026572:48192-48211 None:intergenic 40.0%
! CCAAATTTCCCAGGTGATTT+TGG + tig0026572:48189-48208 Msa1429860:intron 40.0%
GGTGCTTCTATTAACAAAGG+TGG + tig0026572:48390-48409 Msa1429860:intron 40.0%
CAAAGATGGGAAAAGCAAGT+TGG + tig0026572:48489-48508 Msa1429860:CDS 40.0%
!!! TGTGTTGTTTTGATTTGCCG+TGG - tig0026572:48583-48602 None:intergenic 40.0%
CGGCAAATCAAAACAACACA+TGG + tig0026572:48583-48602 Msa1429860:CDS 40.0%
TTGTTTAGATCGATCGATGC+TGG + tig0026572:48615-48634 Msa1429860:CDS 40.0%
!! GCTGAAAAAGGTCTTGTTAC+TGG + tig0026572:48675-48694 Msa1429860:CDS 40.0%
TCGTCGAATAGCGTTTATGT+AGG - tig0026572:48736-48755 None:intergenic 40.0%
ATCTTCAGTGACAACTCCTT+TGG - tig0026572:48864-48883 None:intergenic 40.0%
AGATTGGCAAAGAAGGACTA+TGG + tig0026572:48983-49002 Msa1429860:CDS 40.0%
GTTGAAGCCCTTCAAAGAAA+AGG + tig0026572:49026-49045 Msa1429860:CDS 40.0%
TGACAAGTAATCTCTTGGGT+TGG - tig0026572:49060-49079 None:intergenic 40.0%
AGAGACAGTGAGATTGCAAT+AGG + tig0026572:49514-49533 Msa1429860:CDS 40.0%
TTACTGGTGGCTATATGGTT+TGG - tig0026572:49550-49569 None:intergenic 40.0%
GTCTATTACTGGTGGCTATA+TGG - tig0026572:49555-49574 None:intergenic 40.0%
!! TTTTGGTGGTCTATTACTGG+TGG - tig0026572:49563-49582 None:intergenic 40.0%
ACCAGTAATAGACCACCAAA+AGG + tig0026572:49562-49581 Msa1429860:CDS 40.0%
!! TCCTTTTGGTGGTCTATTAC+TGG - tig0026572:49566-49585 None:intergenic 40.0%
ATGGATTTAGACGTGCACTA+TGG + tig0026572:49593-49612 Msa1429860:CDS 40.0%
TATGAGCACCTTGGAGATAT+TGG + tig0026572:49616-49635 Msa1429860:CDS 40.0%
! ATCGTTTTGCTGAGACCAAT+TGG + tig0026572:49692-49711 Msa1429860:CDS 40.0%
! TCGTTTTGCTGAGACCAATT+GGG + tig0026572:49693-49712 Msa1429860:CDS 40.0%
AAATCCTGGAAGAGCAGTTA+TGG - tig0026572:49803-49822 None:intergenic 40.0%
ACATTCAGTTTCTCTCCACA+TGG - tig0026572:49901-49920 None:intergenic 40.0%
CATGGAAATGGAGGTTAAGG+AGG - tig0026572:46477-46496 None:intergenic 45.0%
GCAGAGATCGAAATTGCATC+CGG - tig0026572:46691-46710 None:intergenic 45.0%
! GATATACCTCTGGCTTGCAT+TGG - tig0026572:47017-47036 None:intergenic 45.0%
GAATGATAGGTAGCCCGAAA+TGG + tig0026572:47233-47252 Msa1429860:CDS 45.0%
CAATTAGAGTCGCTCCAATG+GGG - tig0026572:47327-47346 None:intergenic 45.0%
CCAATTAGAGTCGCTCCAAT+GGG - tig0026572:47328-47347 None:intergenic 45.0%
TCCAATTAGAGTCGCTCCAA+TGG - tig0026572:47329-47348 None:intergenic 45.0%
GTGGATCATCGTCCAATACA+AGG + tig0026572:47418-47437 Msa1429860:CDS 45.0%
GATCATCGTCCAATACAAGG+CGG + tig0026572:47421-47440 Msa1429860:CDS 45.0%
TCACAAGGACTAAAGAGTCC+AGG + tig0026572:47499-47518 Msa1429860:CDS 45.0%
TGAGGAACTCCTTGAAATCC+TGG - tig0026572:47520-47539 None:intergenic 45.0%
TACCGTTCTACTCCATCCAA+TGG - tig0026572:47625-47644 None:intergenic 45.0%
GTATGTGCCGGGAAAATGTT+GGG + tig0026572:47642-47661 Msa1429860:CDS 45.0%
GGTGGGATTGATCTTTGTGA+TGG + tig0026572:48096-48115 Msa1429860:CDS 45.0%
ATATCATGCCATGGCTCTCT+AGG - tig0026572:48417-48436 None:intergenic 45.0%
AACTAGAAGGACCTGTTGCA+TGG + tig0026572:48445-48464 Msa1429860:CDS 45.0%
ACTAGAAGGACCTGTTGCAT+GGG + tig0026572:48446-48465 Msa1429860:CDS 45.0%
CGAAACATGCTTGAAGGTAG+TGG - tig0026572:48528-48547 None:intergenic 45.0%
TCGTGTATCGAATGTTGCCA+CGG + tig0026572:48563-48582 Msa1429860:CDS 45.0%
TGCTTTGCACCTCATACCAA+AGG + tig0026572:48845-48864 Msa1429860:CDS 45.0%
GTGACAACTCCTTTGGTATG+AGG - tig0026572:48857-48876 None:intergenic 45.0%
! GCACTTTCAGGTATACCTTC+TGG - tig0026572:48945-48964 None:intergenic 45.0%
! GCTTGAACTGAAGCACTTTC+AGG - tig0026572:48957-48976 None:intergenic 45.0%
TTATTGCCTTGGAAACCGAG+AGG + tig0026572:49082-49101 Msa1429860:CDS 45.0%
TATTGCCTTGGAAACCGAGA+GGG + tig0026572:49083-49102 Msa1429860:CDS 45.0%
!! TGGAGGAAGGTACTCAGATT+TGG - tig0026572:49863-49882 None:intergenic 45.0%
AAGGGAACATGCACAAGCGT+GGG + tig0026572:46946-46965 Msa1429860:intron 50.0%
TCAATGCCAATGCAAGCCAG+AGG + tig0026572:47008-47027 Msa1429860:CDS 50.0%
CCCATTGGAGCGACTCTAAT+TGG + tig0026572:47325-47344 Msa1429860:CDS 50.0%
TGGAGTAGAACGGTATGTGC+CGG + tig0026572:47630-47649 Msa1429860:CDS 50.0%
GGTATGTGCCGGGAAAATGT+TGG + tig0026572:47641-47660 Msa1429860:CDS 50.0%
AAGAAGCTCCCCAAGAGTGA+TGG - tig0026572:47779-47798 None:intergenic 50.0%
TTCCATCACCAGGGTTACGT+GGG - tig0026572:47954-47973 None:intergenic 50.0%
CACAACACATCCCATGCAAC+AGG - tig0026572:48459-48478 None:intergenic 50.0%
CGGCTTCTGTTGGATCTTGA+GGG - tig0026572:48656-48675 None:intergenic 50.0%
!!! CCTTTTTCAGCGGCTTCTGT+TGG - tig0026572:48666-48685 None:intergenic 50.0%
CCAACAGAAGCCGCTGAAAA+AGG + tig0026572:48663-48682 Msa1429860:CDS 50.0%
GTCATTCCAATGTGGCCAGA+AGG + tig0026572:48927-48946 Msa1429860:CDS 50.0%
GGTATACCTTCTGGCCACAT+TGG - tig0026572:48936-48955 None:intergenic 50.0%
TCTTACCCTCTCGGTTTCCA+AGG - tig0026572:49091-49110 None:intergenic 50.0%
GCACTATGGTATGAGCACCT+TGG + tig0026572:49607-49626 Msa1429860:CDS 50.0%
GGAACCATAACTGCTCTTCC+AGG + tig0026572:49796-49815 Msa1429860:CDS 50.0%
AGGTGGTGAGAATTGGAGGA+AGG - tig0026572:49876-49895 None:intergenic 50.0%
CATGGCTAGGTGGTGAGAAT+TGG - tig0026572:49883-49902 None:intergenic 50.0%
ATTCTCACCACCTAGCCATG+TGG + tig0026572:49883-49902 Msa1429860:exon 50.0%
TTTCTCTCCACATGGCTAGG+TGG - tig0026572:49893-49912 None:intergenic 50.0%
CAGTTTCTCTCCACATGGCT+AGG - tig0026572:49896-49915 None:intergenic 50.0%
CAAGGGAACATGCACAAGCG+TGG + tig0026572:46945-46964 Msa1429860:intron 55.0%
AGGGAACATGCACAAGCGTG+GGG + tig0026572:46947-46966 Msa1429860:intron 55.0%
GGAGTAGAACGGTATGTGCC+GGG + tig0026572:47631-47650 Msa1429860:CDS 55.0%
! GTTTTGTGCCCACGTAACCC+TGG + tig0026572:47943-47962 Msa1429860:CDS 55.0%
CTTCCATCACCAGGGTTACG+TGG - tig0026572:47955-47974 None:intergenic 55.0%
AAGGTGGTCCTAGAGAGCCA+TGG + tig0026572:48406-48425 Msa1429860:CDS 55.0%
!! ATCGATGCTGGTGCTGCTTC+TGG + tig0026572:48627-48646 Msa1429860:CDS 55.0%
GCGGCTTCTGTTGGATCTTG+AGG - tig0026572:48657-48676 None:intergenic 55.0%
! TCAGGTTCTGGTGCCTCTGT+AGG - tig0026572:49131-49150 None:intergenic 55.0%
GGCTAGGTGGTGAGAATTGG+AGG - tig0026572:49880-49899 None:intergenic 55.0%
TGCCCACGTAACCCTGGTGA+TGG + tig0026572:47949-47968 Msa1429860:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0026572 gene 46448 50062 46448 ID=Msa1429860;Name=Msa1429860
tig0026572 mRNA 46448 50062 46448 ID=Msa1429860-mRNA-1;Parent=Msa1429860;Name=Msa1429860-mRNA-1;_AED=0.04;_eAED=0.04;_QI=83|1|1|1|0.4|0.33|6|165|826
tig0026572 exon 46448 46495 46448 ID=Msa1429860-mRNA-1:exon:6698;Parent=Msa1429860-mRNA-1
tig0026572 exon 46586 46716 46586 ID=Msa1429860-mRNA-1:exon:6699;Parent=Msa1429860-mRNA-1
tig0026572 exon 46949 47029 46949 ID=Msa1429860-mRNA-1:exon:6700;Parent=Msa1429860-mRNA-1
tig0026572 exon 47172 48201 47172 ID=Msa1429860-mRNA-1:exon:6701;Parent=Msa1429860-mRNA-1
tig0026572 exon 48391 49218 48391 ID=Msa1429860-mRNA-1:exon:6702;Parent=Msa1429860-mRNA-1
tig0026572 exon 49452 50062 49452 ID=Msa1429860-mRNA-1:exon:6703;Parent=Msa1429860-mRNA-1
tig0026572 five_prime_UTR 46448 46495 46448 ID=Msa1429860-mRNA-1:five_prime_utr;Parent=Msa1429860-mRNA-1
tig0026572 five_prime_UTR 46586 46620 46586 ID=Msa1429860-mRNA-1:five_prime_utr;Parent=Msa1429860-mRNA-1
tig0026572 CDS 46621 46716 46621 ID=Msa1429860-mRNA-1:cds;Parent=Msa1429860-mRNA-1
tig0026572 CDS 46949 47029 46949 ID=Msa1429860-mRNA-1:cds;Parent=Msa1429860-mRNA-1
tig0026572 CDS 47172 48201 47172 ID=Msa1429860-mRNA-1:cds;Parent=Msa1429860-mRNA-1
tig0026572 CDS 48391 49218 48391 ID=Msa1429860-mRNA-1:cds;Parent=Msa1429860-mRNA-1
tig0026572 CDS 49452 49897 49452 ID=Msa1429860-mRNA-1:cds;Parent=Msa1429860-mRNA-1
tig0026572 three_prime_UTR 49898 50062 49898 ID=Msa1429860-mRNA-1:three_prime_utr;Parent=Msa1429860-mRNA-1
Gene Sequence

>Msa1429860

ATGTTGCACTTACTACACGGGAAGCTTGATGTGACTATATATGAGGTCGATACACTACAAACTCTTGCCGGATGCAATTTCGATCTCTGCAACAAGGGAACATGCACAAGCGTGGGGAAGAAAATTCTGTCACAAGTCAAGAGTTGTTTTCTATGTCAATGCCAATGCAAGCCAGAGATTATTGGGATGGGTCTATATGCAACAGTTGATTTAGACAAAGCAAGAGTTGGAAGAACTAGAATGATAGGTAGCCCGAAATGGAATGAAACCTTTCACATTTACTCTGCACATTCAATCTCCAACATTATATTCACTGTCAAACAAGATAACCCCATTGGAGCGACTCTAATTGGAAGAGCTTATGTTCCAGTTGAACAAGTCATAAAGGGCAACATAGTGAATACATGGGTTAATATATTAGATGTGGATCATCGTCCAATACAAGGCGGTTCCAAAATCCATGTTCAAATACAATTCTCACATGTTAAAAATGATCCAAACTGGTCACAAGGACTAAAGAGTCCAGGATTTCAAGGAGTTCCTCACACATTCTTTAAACAAAATAATGGTTGCCAAATTACTCTTTACCAAGATGCACATGTTCTAGATGGTTCTGTTCCGTATATTCCATTGGATGGAGTAGAACGGTATGTGCCGGGAAAATGTTGGGAAGATACTTACAATGCAATTAATGATGCTAAGCATTTTATTTGCATAACTGGTTGGTCTGTTTACACGGAAATAACCTTGATTAGAGATCCAAACAAGTCAACAAGAACAACCATCACTCTTGGGGAGCTTCTTAAGAAGAAAGCCAATGAAGGTGTTAATGTTCTTATGCTTGTTTGGGATGACAGAACTTCTGTTCCTGACTTTAAGAAAGATGGCTTGATGGCAACTCATGATCAAGAAATAGCAGAGTATTTTAGAAACACAAATGTGCATTGTGTTTTGTGCCCACGTAACCCTGGTGATGGAAGAAGTATAGTTCAAGGTTTTGAAATTTCAACTATGTTTACTCATCACCAGAAGACTATAATTGTTGACAGCAAAGTTGCTAGTTCTGCACAATGGAAGAAGAGAACAATCACAAGTTTTGTTGGTGGGATTGATCTTTGTGATGGGAGATATGATACCATGGAACATCCTTTGTTTTCAACTTTGAATACAATTCATCATGATGATTTTCATCAACCAAATTTCCCAGGTGCTTCTATTAACAAAGGTGGTCCTAGAGAGCCATGGCATGATATTCATTGTAAACTAGAAGGACCTGTTGCATGGGATGTGTTGTGCAATTTTGAGCAAAGATGGGAAAAGCAAGTTGGAAAGCAATTAATTCCACTACCTTCAAGCATGTTTCGTGAATATGATATTGCTCGTGTATCGAATGTTGCCACGGCAAATCAAAACAACACATGGAATGTTCAATTGTTTAGATCGATCGATGCTGGTGCTGCTTCTGGCTTCCCTCAAGATCCAACAGAAGCCGCTGAAAAAGGTCTTGTTACTGGAAAAGATAACATCATTGATAGAAGCATTCAAGATGCCTACATAAACGCTATTCGACGAGCTAAGAACTTCGTCTACATTGAAAATCAGTATTTTCTAGGTAGTTCATATGGTTGGAAATCATCGGATATTAAAGTTGAGGATATTGGTGCTTTGCACCTCATACCAAAGGAGTTGTCACTGAAGATTGTTAGCAAGATTGAAGCTGGAGAGAGATTCTCTGTGTACATTGTCATTCCAATGTGGCCAGAAGGTATACCTGAAAGTGCTTCAGTTCAAGCAATATTAGATTGGCAAAGAAGGACTATGGAGATGATGTATTCTGACATAGTTGAAGCCCTTCAAAGAAAAGGAATCAGAGCCAACCCAAGAGATTACTTGTCATTTTATTGCCTTGGAAACCGAGAGGGTAAGAAAATGAATGAATATACTCCTACAGAGGCACCAGAACCTGACAGTGATTATATTAGAGCACAAAATTCTAGAAGATTCATGATATATGTTCATGCCAAAATGATGATAGTTGATGATGAATACATAATCATTGGTTCTGCCAACATAAATCAAAGATCAATGGATGGTGCTAGAGACAGTGAGATTGCAATAGGTGCATTTCAACCAAACCATATAGCCACCAGTAATAGACCACCAAAAGGACAAATATATGGATTTAGACGTGCACTATGGTATGAGCACCTTGGAGATATTGGTGATACAAGCTTCTTTGAAAATCCAGAAAGTTTGAATTGCATTAAGCTTGTAAATCGTTTTGCTGAGACCAATTGGGACATATACTCAAAGGATGCATTTGATGAATACAGATCATTTCATCACCTCATGCATTATCCAATACAAGTAACCAATAATGGAACCATAACTGCTCTTCCAGGATTTGAATGTTTTCCTGATACTAAGGCTAAGATTCTAGGTTCCAAATCTGAGTACCTTCCTCCAATTCTCACCACCTAG
Protein sequence

>Msa1429860

MLHLLHGKLDVTIYEVDTLQTLAGCNFDLCNKGTCTSVGKKILSQVKSCFLCQCQCKPEIIGMGLYATVDLDKARVGRTRMIGSPKWNETFHIYSAHSISNIIFTVKQDNPIGATLIGRAYVPVEQVIKGNIVNTWVNILDVDHRPIQGGSKIHVQIQFSHVKNDPNWSQGLKSPGFQGVPHTFFKQNNGCQITLYQDAHVLDGSVPYIPLDGVERYVPGKCWEDTYNAINDAKHFICITGWSVYTEITLIRDPNKSTRTTITLGELLKKKANEGVNVLMLVWDDRTSVPDFKKDGLMATHDQEIAEYFRNTNVHCVLCPRNPGDGRSIVQGFEISTMFTHHQKTIIVDSKVASSAQWKKRTITSFVGGIDLCDGRYDTMEHPLFSTLNTIHHDDFHQPNFPGASINKGGPREPWHDIHCKLEGPVAWDVLCNFEQRWEKQVGKQLIPLPSSMFREYDIARVSNVATANQNNTWNVQLFRSIDAGAASGFPQDPTEAAEKGLVTGKDNIIDRSIQDAYINAIRRAKNFVYIENQYFLGSSYGWKSSDIKVEDIGALHLIPKELSLKIVSKIEAGERFSVYIVIPMWPEGIPESASVQAILDWQRRTMEMMYSDIVEALQRKGIRANPRDYLSFYCLGNREGKKMNEYTPTEAPEPDSDYIRAQNSRRFMIYVHAKMMIVDDEYIIIGSANINQRSMDGARDSEIAIGAFQPNHIATSNRPPKGQIYGFRRALWYEHLGDIGDTSFFENPESLNCIKLVNRFAETNWDIYSKDAFDEYRSFHHLMHYPIQVTNNGTITALPGFECFPDTKAKILGSKSEYLPPILTT*