AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562560 MtrunA17_Chr8g0375991 97.642 212 5 0 1 212 1 212 1.89e-152 421
Msa0562560 MtrunA17_Chr1g0161941 59.048 210 84 2 1 208 1 210 2.53e-85 251
Msa0562560 MtrunA17_Chr1g0161921 43.750 208 114 3 1 206 7 213 4.39e-57 180
Msa0562560 MtrunA17_Chr1g0203371 39.604 202 117 2 9 205 13 214 2.55e-53 171
Msa0562560 MtrunA17_Chr6g0456901 36.869 198 119 3 11 202 16 213 2.41e-40 137
Msa0562560 MtrunA17_Chr5g0422371 35.079 191 111 6 10 189 17 205 1.12e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562560 AT1G14730.1 63.107 206 73 3 6 209 17 221 2.98e-88 259
Msa0562560 AT4G25570.1 38.571 210 124 2 1 205 5 214 8.24e-50 162
Msa0562560 AT4G25570.2 38.571 210 124 2 1 205 46 255 1.01e-48 160
Msa0562560 AT5G38630.1 37.073 205 123 3 8 206 13 217 1.30e-43 146
Msa0562560 AT1G26100.1 34.104 173 102 5 8 177 13 176 9.04e-24 95.1

Find 112 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTATAGTACCTCAATTT+TGG 0.192371 4_1:+62505545 Msa0562560:CDS
GCCTTGAACACGTGCGCTTC+TGG 0.194316 4_1:+62507972 Msa0562560:three_prime_UTR
CATTTCATTGCCATAGTTCT+TGG 0.208807 4_1:+62506632 Msa0562560:CDS
ACCTCCTCCTTCTTCATTCC+TGG 0.231179 4_1:+62507942 Msa0562560:three_prime_UTR
TTCATAATTAAAATACAAAT+TGG 0.236533 4_1:+62507522 Msa0562560:three_prime_UTR
CCCTCATCCTTATGCTTGTT+TGG 0.262566 4_1:+62505589 Msa0562560:CDS
CACTTCAAGAATAAACAATA+TGG 0.263290 4_1:-62507305 None:intergenic
GGTCTAGCAATTCTCTTGTT+TGG 0.264168 4_1:+62507252 Msa0562560:CDS
TTCACTTTCCTCTTCCTTCC+CGG 0.285952 4_1:-62508039 None:intergenic
CGAGAACATTCTGACGCATT+TGG 0.304248 4_1:-62508343 None:intergenic
ATGTTTACAGTCTCCATTCA+TGG 0.305345 4_1:+62506708 Msa0562560:CDS
ATTGCCTGAGAATGCTAAAA+TGG 0.321169 4_1:-62507793 None:intergenic
AGATGTGATGAGGTATTTAA+AGG 0.326399 4_1:+62508259 Msa0562560:three_prime_UTR
GCAATTTGTGCTGCACTAAC+AGG 0.327192 4_1:+62507159 Msa0562560:CDS
GCCATAGTTCTTGGAATTGT+TGG 0.333746 4_1:+62506641 Msa0562560:CDS
TGATGAGACGGGATTCTCTT+TGG 0.342183 4_1:-62507221 None:intergenic
TCGCTGATTCCAAGTTTCTT+TGG 0.347529 4_1:-62508143 None:intergenic
AAGGAAGAGGAAAGTGAATT+GGG 0.348719 4_1:+62508044 Msa0562560:three_prime_UTR
GTGTTCAAGGCGGATCTTCC+AGG 0.349493 4_1:-62507960 None:intergenic
TAAATGCAAAAGCTAACTAT+AGG 0.353480 4_1:-62505479 None:intergenic
CTCCATTCATGGATTGGCCT+TGG 0.364585 4_1:+62506719 Msa0562560:CDS
ATCCATTCTCCCTCGACGTA+TGG 0.374390 4_1:-62508091 None:intergenic
AGGTAGAGATTGAAGATGAT+AGG 0.379460 4_1:-62507917 None:intergenic
TGCTGCACTAACAGGTTTGA+TGG 0.381294 4_1:+62507167 Msa0562560:CDS
TACAGTCTCCATTCATGGAT+TGG 0.391717 4_1:+62506713 Msa0562560:CDS
ACCAACAATTCCAAGAACTA+TGG 0.394185 4_1:-62506642 None:intergenic
GTAATAAAGAGAATGTAATT+TGG 0.407285 4_1:-62507593 None:intergenic
TTCTGTGTTTCATGTGTGCC+TGG 0.408328 4_1:-62506590 None:intergenic
TGTGGATATGACAGTTGGTT+TGG 0.414085 4_1:+62507281 Msa0562560:CDS
GAAGGAAGAGGAAAGTGAAT+TGG 0.416907 4_1:+62508043 Msa0562560:three_prime_UTR
GGCTTCTTAATCTCTTCCTT+AGG 0.419191 4_1:+62507723 Msa0562560:three_prime_UTR
GGACTGACATTCATGTTTCA+AGG 0.423824 4_1:+62507069 Msa0562560:CDS
CCTCTGCCACCAAAGAAACT+TGG 0.426213 4_1:+62508134 Msa0562560:three_prime_UTR
TGTTGTTCATTAAAATGAAA+TGG 0.426492 4_1:+62507433 Msa0562560:three_prime_UTR
GTTAAGACCATTGATATCTC+TGG 0.432223 4_1:-62507696 None:intergenic
ATATCAATGGTCTTAACTTC+AGG 0.435827 4_1:+62507702 Msa0562560:three_prime_UTR
AAATGCAAAAGCTAACTATA+GGG 0.438376 4_1:-62505478 None:intergenic
AACCCAAAGAACATCAGAAA+AGG 0.444479 4_1:-62505805 None:intergenic
AATGAAGAAGGAGGAGGTGA+AGG 0.451265 4_1:-62507937 None:intergenic
TAGAAAATTCTATAACTGTC+TGG 0.452298 4_1:+62508308 Msa0562560:three_prime_UTR
TCCCATACGTCGAGGGAGAA+TGG 0.455246 4_1:+62508089 Msa0562560:three_prime_UTR
TCCAGAAGCGCACGTGTTCA+AGG 0.455331 4_1:-62507973 None:intergenic
CCAAGTTTCTTTGGTGGCAG+AGG 0.460257 4_1:-62508134 None:intergenic
CGACGTATGGGATCCATTCA+AGG 0.470358 4_1:-62508078 None:intergenic
TCCATTCTCCCTCGACGTAT+GGG 0.473050 4_1:-62508090 None:intergenic
CTGATTCCAAGTTTCTTTGG+TGG 0.478672 4_1:-62508140 None:intergenic
ACTTCAAGAATAAACAATAT+GGG 0.488825 4_1:-62507304 None:intergenic
GGTAGAGATTGAAGATGATA+GGG 0.493419 4_1:-62507916 None:intergenic
TTGCGAGCACACGTGTGGAC+TGG 0.496964 4_1:-62508004 None:intergenic
GAGGTATTTAAAGGAAATAG+AGG 0.500432 4_1:+62508268 Msa0562560:three_prime_UTR
CCTCACTGTGACTGTGCCTA+AGG 0.507634 4_1:-62507739 None:intergenic
GTAGCAGTGGAAAGTTTATG+AGG 0.516165 4_1:-62507824 None:intergenic
CTGCAAGCCAAACAAGCAAA+AGG 0.518582 4_1:-62506744 None:intergenic
GCAGCAACCAAACAAGCATA+AGG 0.521345 4_1:-62505596 None:intergenic
CAACCAAGTTCACCATATCA+TGG 0.523417 4_1:-62506685 None:intergenic
AAGAAGACAAGAATGATCAA+TGG 0.523834 4_1:-62507860 None:intergenic
AAGACAATCAAGGGCTGCAA+TGG 0.529021 4_1:+62507095 Msa0562560:CDS
TGTTTAACCAGAGATATCAA+TGG 0.529039 4_1:+62507689 Msa0562560:three_prime_UTR
ACCAGTAAAATTGATGAGAC+GGG 0.529605 4_1:-62507232 None:intergenic
ATGTCTTACCACACAGTACC+AGG 0.530006 4_1:+62506572 Msa0562560:CDS
CATATCATGGAACTTGAAAA+CGG 0.532163 4_1:-62506672 None:intergenic
CACTAACAGGTTTGATGGAG+AGG 0.533891 4_1:+62507172 Msa0562560:CDS
GACCAGTAAAATTGATGAGA+CGG 0.537528 4_1:-62507233 None:intergenic
TTCAAGGAACAAGACAATCA+AGG 0.538249 4_1:+62507085 Msa0562560:CDS
TGTATAAGAAGATAGATTTG+AGG 0.540133 4_1:-62507337 None:intergenic
TGCCAAGGCCAATCCATGAA+TGG 0.541924 4_1:-62506721 None:intergenic
ATGGCTCCATGGCACATAGC+TGG 0.542007 4_1:+62507114 Msa0562560:CDS
GTTCCATGATATGGTGAACT+TGG 0.544949 4_1:+62506682 Msa0562560:CDS
ACCAAACAAGCATAAGGATG+AGG 0.552123 4_1:-62505590 None:intergenic
AGTTCTGTGTTATGTTAGAA+TGG 0.552597 4_1:-62507658 None:intergenic
CAAGGGCTGCAATGGCTCCA+TGG 0.553808 4_1:+62507103 Msa0562560:CDS
ACTATAAGAACGCATCCTGC+TGG 0.555133 4_1:-62505532 None:intergenic
ACCCAAAGAACATCAGAAAA+GGG 0.558431 4_1:-62505804 None:intergenic
GGGAATGGAAGATCCTTGAA+TGG 0.560868 4_1:+62508065 Msa0562560:three_prime_UTR
GGATCAAGTGAAAGCTGCAA+TGG 0.563638 4_1:-62507772 None:intergenic
CAAACAAGCAAAAGGTGCCA+AGG 0.564883 4_1:-62506736 None:intergenic
ACATTCTGACGCATTTGGCG+CGG 0.570484 4_1:-62508338 None:intergenic
CTACCACCAGCTATGTGCCA+TGG 0.572574 4_1:-62507120 None:intergenic
GAGGAAAGTGAATTGGGGAA+TGG 0.573543 4_1:+62508050 Msa0562560:three_prime_UTR
TCTTTGGTGGCAGAGGCAGA+AGG 0.574012 4_1:-62508127 None:intergenic
TCAAGGAACAAGACAATCAA+GGG 0.574140 4_1:+62507086 Msa0562560:CDS
GAGGGGATTGAATATGACTC+TGG 0.574307 4_1:+62505627 Msa0562560:CDS
TGGTTGCTGCATTACCGTGA+GGG 0.574869 4_1:+62505609 Msa0562560:CDS
GTGAAAGCTGCAATGGAGAA+TGG 0.576830 4_1:-62507765 None:intergenic
GGTTGCTGCATTACCGTGAG+GGG 0.582408 4_1:+62505610 Msa0562560:CDS
CGTATACTGTAGTAACCAGC+AGG 0.585010 4_1:+62505517 Msa0562560:five_prime_UTR
CATCGCGTGGAGCGTAGCAG+TGG 0.585473 4_1:-62507837 None:intergenic
AGACGGGATTCTCTTTGGTG+TGG 0.594078 4_1:-62507216 None:intergenic
GGATCTTCCAGGAATGAAGA+AGG 0.594776 4_1:-62507949 None:intergenic
CATACATTGAAAACACGAAG+TGG 0.594783 4_1:-62505654 None:intergenic
AGCATTTGCGAGCACACGTG+TGG 0.598239 4_1:-62508009 None:intergenic
AAACAAGTGAGCCAAAATTG+AGG 0.600449 4_1:-62505556 None:intergenic
TATAAGTTGATTGATTCATG+TGG 0.605545 4_1:+62507488 Msa0562560:three_prime_UTR
CCAAACAAGCATAAGGATGA+GGG 0.611153 4_1:-62505589 None:intergenic
TCCAGGAATGAAGAAGGAGG+AGG 0.612205 4_1:-62507943 None:intergenic
GAATGGATCCCATACGTCGA+GGG 0.618471 4_1:+62508082 Msa0562560:three_prime_UTR
GCAGTGGAAAGTTTATGAGG+AGG 0.629631 4_1:-62507821 None:intergenic
GAATGATCAATGGCATCGCG+TGG 0.629958 4_1:-62507850 None:intergenic
AGGAAGAGGAAAGTGAATTG+GGG 0.634584 4_1:+62508045 Msa0562560:three_prime_UTR
GAGCACACGTGTGGACTGGA+AGG 0.634770 4_1:-62508000 None:intergenic
AGTCATATTCAATCCCCTCA+CGG 0.654951 4_1:-62505623 None:intergenic
AATATATACCTTGACCAACA+AGG 0.656313 4_1:-62505834 None:intergenic
TGGTAAGAGGAGATGTGATG+AGG 0.666845 4_1:+62508249 Msa0562560:three_prime_UTR
GCTCCATGGCACATAGCTGG+TGG 0.673506 4_1:+62507117 Msa0562560:CDS
TCTTCCAGGAATGAAGAAGG+AGG 0.674300 4_1:-62507946 None:intergenic
TGAATGGATCCCATACGTCG+AGG 0.681788 4_1:+62508081 Msa0562560:three_prime_UTR
TTGGTTGCTGCATTACCGTG+AGG 0.689331 4_1:+62505608 Msa0562560:CDS
GATAGGGTTCTTCAGATCAG+CGG 0.712601 4_1:-62507900 None:intergenic
CATGTGTGCCTGGTACTGTG+TGG 0.717385 4_1:-62506580 None:intergenic
CCTTAGGCACAGTCACAGTG+AGG 0.723797 4_1:+62507739 Msa0562560:three_prime_UTR
AGAAGCGCACGTGTTCAAGG+CGG 0.739000 4_1:-62507970 None:intergenic
GCTGGTTACTACAGTATACG+AGG 0.741619 4_1:-62505514 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTAGCTTTAAAATATA+AGG - chr4_1:62506240-62506259 None:intergenic 10.0%
!! TTCATAATTAAAATACAAAT+TGG + chr4_1:62507522-62507541 Msa0562560:three_prime_UTR 10.0%
!!! AATTTTTTATACTTAACAAC+TGG + chr4_1:62505702-62505721 Msa0562560:intron 15.0%
!!! TTTATTTCAAGTTTTAAGAT+AGG + chr4_1:62505858-62505877 Msa0562560:intron 15.0%
!! TAAGAAACAAAGTAAAAATT+AGG + chr4_1:62506419-62506438 Msa0562560:intron 15.0%
!! AAGAAACAAAGTAAAAATTA+GGG + chr4_1:62506420-62506439 Msa0562560:intron 15.0%
!!! AATTTTTTATACCTAACAAC+TGG + chr4_1:62505737-62505756 Msa0562560:intron 20.0%
!! TAAGAAATATGCAAATTCTA+TGG - chr4_1:62506207-62506226 None:intergenic 20.0%
!! TTACAGTTAAAAATAGAAAC+TGG + chr4_1:62506449-62506468 Msa0562560:intron 20.0%
!! TATTTCAGTTTACTAGTATT+AGG - chr4_1:62506490-62506509 None:intergenic 20.0%
!!! AATATAAACTATTTTTGCAG+TGG + chr4_1:62507040-62507059 Msa0562560:intron 20.0%
!! ACTTCAAGAATAAACAATAT+GGG - chr4_1:62507307-62507326 None:intergenic 20.0%
!! ACATATAAAAAGAAGAATGT+TGG - chr4_1:62507394-62507413 None:intergenic 20.0%
!! TGTTGTTCATTAAAATGAAA+TGG + chr4_1:62507433-62507452 Msa0562560:three_prime_UTR 20.0%
!! GTAATAAAGAGAATGTAATT+TGG - chr4_1:62507596-62507615 None:intergenic 20.0%
!!! ATTTTTATTTTGTTGGTAAG+AGG + chr4_1:62508236-62508255 Msa0562560:three_prime_UTR 20.0%
!! TGTTATGACACAAAAGTTTT+TGG - chr4_1:62505454-62505473 None:intergenic 25.0%
! AAATGCAAAAGCTAACTATA+GGG - chr4_1:62505481-62505500 None:intergenic 25.0%
! TAAATGCAAAAGCTAACTAT+AGG - chr4_1:62505482-62505501 None:intergenic 25.0%
!! TTCTTATAGTACCTCAATTT+TGG + chr4_1:62505545-62505564 Msa0562560:CDS 25.0%
!!! ATTTCAAGTTTTAAGATAGG+TGG + chr4_1:62505861-62505880 Msa0562560:intron 25.0%
!!! TGTAAACTTCTACTTTTCAA+AGG - chr4_1:62505932-62505951 None:intergenic 25.0%
!!! TTCAAACGATTTTGAAGTAT+TGG + chr4_1:62506156-62506175 Msa0562560:intron 25.0%
!!! TTTTGAAGTATTGGTCTATT+TGG + chr4_1:62506165-62506184 Msa0562560:intron 25.0%
! AATTACTTGACTGATTGAAT+TGG + chr4_1:62506525-62506544 Msa0562560:intron 25.0%
! TTGAATTTAACCAAAAAGCA+TGG + chr4_1:62506774-62506793 Msa0562560:intron 25.0%
! TAAATTTAATAGAATGTCGC+TGG - chr4_1:62506999-62507018 None:intergenic 25.0%
! CACTTCAAGAATAAACAATA+TGG - chr4_1:62507308-62507327 None:intergenic 25.0%
! TGTATAAGAAGATAGATTTG+AGG - chr4_1:62507340-62507359 None:intergenic 25.0%
!!! TATAAGTTGATTGATTCATG+TGG + chr4_1:62507488-62507507 Msa0562560:three_prime_UTR 25.0%
!!! GACATTTTTCTTTAACTTCT+TGG + chr4_1:62508164-62508183 Msa0562560:three_prime_UTR 25.0%
! TAGAAAATTCTATAACTGTC+TGG + chr4_1:62508308-62508327 Msa0562560:three_prime_UTR 25.0%
! TGAATTTTTCTCCATTATGC+AGG + chr4_1:62505777-62505796 Msa0562560:intron 30.0%
AATATATACCTTGACCAACA+AGG - chr4_1:62505837-62505856 None:intergenic 30.0%
ATATTTACATCGAGACATTG+AGG + chr4_1:62505955-62505974 Msa0562560:intron 30.0%
! GTTCTTCTTGTTATAACATG+TGG - chr4_1:62506090-62506109 None:intergenic 30.0%
! ACTTTGGAAGTAGAAGTTTA+TGG - chr4_1:62506112-62506131 None:intergenic 30.0%
AAATCGTTTGAAAACCTGAA+TGG - chr4_1:62506148-62506167 None:intergenic 30.0%
GAAATATGCAAATTCTATGG+TGG - chr4_1:62506204-62506223 None:intergenic 30.0%
AGTCAAGTAATTGATATCTG+AGG - chr4_1:62506517-62506536 None:intergenic 30.0%
CATATCATGGAACTTGAAAA+CGG - chr4_1:62506675-62506694 None:intergenic 30.0%
AGTTCTGTGTTATGTTAGAA+TGG - chr4_1:62507661-62507680 None:intergenic 30.0%
TGTTTAACCAGAGATATCAA+TGG + chr4_1:62507689-62507708 Msa0562560:three_prime_UTR 30.0%
! ATATCAATGGTCTTAACTTC+AGG + chr4_1:62507702-62507721 Msa0562560:three_prime_UTR 30.0%
AAGAAGACAAGAATGATCAA+TGG - chr4_1:62507863-62507882 None:intergenic 30.0%
! AGATGTGATGAGGTATTTAA+AGG + chr4_1:62508259-62508278 Msa0562560:three_prime_UTR 30.0%
! GAGGTATTTAAAGGAAATAG+AGG + chr4_1:62508268-62508287 Msa0562560:three_prime_UTR 30.0%
!! TAGAATTTTCTAGCAGAAAC+TGG - chr4_1:62508300-62508319 None:intergenic 30.0%
AAACAAGTGAGCCAAAATTG+AGG - chr4_1:62505559-62505578 None:intergenic 35.0%
!!! TCAATTTTGGCTCACTTGTT+TGG + chr4_1:62505558-62505577 Msa0562560:CDS 35.0%
CATACATTGAAAACACGAAG+TGG - chr4_1:62505657-62505676 None:intergenic 35.0%
! ACCCTTTTCTGATGTTCTTT+GGG + chr4_1:62505803-62505822 Msa0562560:CDS 35.0%
ACCCAAAGAACATCAGAAAA+GGG - chr4_1:62505807-62505826 None:intergenic 35.0%
AACCCAAAGAACATCAGAAA+AGG - chr4_1:62505808-62505827 None:intergenic 35.0%
!! TTTGGGTTCATTTTCCTTGT+TGG + chr4_1:62505820-62505839 Msa0562560:CDS 35.0%
! TTCATTTTCCTTGTTGGTCA+AGG + chr4_1:62505826-62505845 Msa0562560:CDS 35.0%
! TTTCAAGTAATGATCCACGT+CGG - chr4_1:62505888-62505907 None:intergenic 35.0%
CATTTCATTGCCATAGTTCT+TGG + chr4_1:62506632-62506651 Msa0562560:CDS 35.0%
ACCAACAATTCCAAGAACTA+TGG - chr4_1:62506645-62506664 None:intergenic 35.0%
!!! CGTTTTCAAGTTCCATGATA+TGG + chr4_1:62506673-62506692 Msa0562560:CDS 35.0%
ATGTTTACAGTCTCCATTCA+TGG + chr4_1:62506708-62506727 Msa0562560:CDS 35.0%
! TTGTTCAAAGCCATGCTTTT+TGG - chr4_1:62506787-62506806 None:intergenic 35.0%
!!! TGTCGCTGGTTAGTTTTTTA+GGG - chr4_1:62506985-62507004 None:intergenic 35.0%
!!! ATGTCGCTGGTTAGTTTTTT+AGG - chr4_1:62506986-62507005 None:intergenic 35.0%
!!! CTATTTTTGCAGTGGCTTTT+TGG + chr4_1:62507048-62507067 Msa0562560:intron 35.0%
TTCAAGGAACAAGACAATCA+AGG + chr4_1:62507085-62507104 Msa0562560:CDS 35.0%
TCAAGGAACAAGACAATCAA+GGG + chr4_1:62507086-62507105 Msa0562560:CDS 35.0%
!! TGGTAGAGCACTATTTTTCA+TGG + chr4_1:62507137-62507156 Msa0562560:CDS 35.0%
ACCAGTAAAATTGATGAGAC+GGG - chr4_1:62507235-62507254 None:intergenic 35.0%
GACCAGTAAAATTGATGAGA+CGG - chr4_1:62507236-62507255 None:intergenic 35.0%
!! TCTCTTGTTTGGAGTTTTTG+TGG + chr4_1:62507263-62507282 Msa0562560:CDS 35.0%
! GTTTTTGTGGATATGACAGT+TGG + chr4_1:62507276-62507295 Msa0562560:CDS 35.0%
GTTAAGACCATTGATATCTC+TGG - chr4_1:62507699-62507718 None:intergenic 35.0%
!! TGATCCATTTTAGCATTCTC+AGG + chr4_1:62507789-62507808 Msa0562560:three_prime_UTR 35.0%
ATTGCCTGAGAATGCTAAAA+TGG - chr4_1:62507796-62507815 None:intergenic 35.0%
GGTAGAGATTGAAGATGATA+GGG - chr4_1:62507919-62507938 None:intergenic 35.0%
AGGTAGAGATTGAAGATGAT+AGG - chr4_1:62507920-62507939 None:intergenic 35.0%
AAGGAAGAGGAAAGTGAATT+GGG + chr4_1:62508044-62508063 Msa0562560:three_prime_UTR 35.0%
CCAAACAAGCATAAGGATGA+GGG - chr4_1:62505592-62505611 None:intergenic 40.0%
ACCAAACAAGCATAAGGATG+AGG - chr4_1:62505593-62505612 None:intergenic 40.0%
AGTCATATTCAATCCCCTCA+CGG - chr4_1:62505626-62505645 None:intergenic 40.0%
GTAGAATGTGACCAGTTGTT+AGG - chr4_1:62505751-62505770 None:intergenic 40.0%
AAAAGGGTGAACCTGCATAA+TGG - chr4_1:62505791-62505810 None:intergenic 40.0%
! CACCCTTTTCTGATGTTCTT+TGG + chr4_1:62505802-62505821 Msa0562560:CDS 40.0%
! GACATTGAGGTGTTTGATGT+CGG + chr4_1:62505968-62505987 Msa0562560:intron 40.0%
TGGTAAGTGATGTTGCACTT+TGG - chr4_1:62506128-62506147 None:intergenic 40.0%
TGCAACATCACTTACCATTC+AGG + chr4_1:62506131-62506150 Msa0562560:intron 40.0%
! ATTGGTCTATTTGGTCGCTA+TGG + chr4_1:62506174-62506193 Msa0562560:intron 40.0%
GCCATAGTTCTTGGAATTGT+TGG + chr4_1:62506641-62506660 Msa0562560:CDS 40.0%
GTTCCATGATATGGTGAACT+TGG + chr4_1:62506682-62506701 Msa0562560:CDS 40.0%
CAACCAAGTTCACCATATCA+TGG - chr4_1:62506688-62506707 None:intergenic 40.0%
TACAGTCTCCATTCATGGAT+TGG + chr4_1:62506713-62506732 Msa0562560:CDS 40.0%
TGTCCAAAAGATGTAGCGAT+GGG + chr4_1:62506895-62506914 Msa0562560:intron 40.0%
TACGCTGAAAGACTTTCAGA+AGG + chr4_1:62506922-62506941 Msa0562560:intron 40.0%
GGACTGACATTCATGTTTCA+AGG + chr4_1:62507069-62507088 Msa0562560:CDS 40.0%
! TCCCGTCTCATCAATTTTAC+TGG + chr4_1:62507231-62507250 Msa0562560:CDS 40.0%
GGTCTAGCAATTCTCTTGTT+TGG + chr4_1:62507252-62507271 Msa0562560:CDS 40.0%
TGTGGATATGACAGTTGGTT+TGG + chr4_1:62507281-62507300 Msa0562560:CDS 40.0%
GGCTTCTTAATCTCTTCCTT+AGG + chr4_1:62507723-62507742 Msa0562560:three_prime_UTR 40.0%
GTAGCAGTGGAAAGTTTATG+AGG - chr4_1:62507827-62507846 None:intergenic 40.0%
! CTCGCAAATGCTGAATTTTC+CGG + chr4_1:62508020-62508039 Msa0562560:three_prime_UTR 40.0%
! TCGCAAATGCTGAATTTTCC+GGG + chr4_1:62508021-62508040 Msa0562560:three_prime_UTR 40.0%
! AAATGCTGAATTTTCCGGGA+AGG + chr4_1:62508025-62508044 Msa0562560:three_prime_UTR 40.0%
GAAGGAAGAGGAAAGTGAAT+TGG + chr4_1:62508043-62508062 Msa0562560:three_prime_UTR 40.0%
AGGAAGAGGAAAGTGAATTG+GGG + chr4_1:62508045-62508064 Msa0562560:three_prime_UTR 40.0%
CTGATTCCAAGTTTCTTTGG+TGG - chr4_1:62508143-62508162 None:intergenic 40.0%
!! TCGCTGATTCCAAGTTTCTT+TGG - chr4_1:62508146-62508165 None:intergenic 40.0%
GCTGGTTACTACAGTATACG+AGG - chr4_1:62505517-62505536 None:intergenic 45.0%
CGTATACTGTAGTAACCAGC+AGG + chr4_1:62505517-62505536 Msa0562560:five_prime_UTR 45.0%
ACTATAAGAACGCATCCTGC+TGG - chr4_1:62505535-62505554 None:intergenic 45.0%
CCCTCATCCTTATGCTTGTT+TGG + chr4_1:62505589-62505608 Msa0562560:CDS 45.0%
GCAGCAACCAAACAAGCATA+AGG - chr4_1:62505599-62505618 None:intergenic 45.0%
GAGGGGATTGAATATGACTC+TGG + chr4_1:62505627-62505646 Msa0562560:CDS 45.0%
ATGTCTTACCACACAGTACC+AGG + chr4_1:62506572-62506591 Msa0562560:CDS 45.0%
TTCTGTGTTTCATGTGTGCC+TGG - chr4_1:62506593-62506612 None:intergenic 45.0%
!! CAAACAAGCAAAAGGTGCCA+AGG - chr4_1:62506739-62506758 None:intergenic 45.0%
! CTTGGCACCTTTTGCTTGTT+TGG + chr4_1:62506737-62506756 Msa0562560:CDS 45.0%
CTGCAAGCCAAACAAGCAAA+AGG - chr4_1:62506747-62506766 None:intergenic 45.0%
!!! CTTTTGCTTGTTTGGCTTGC+AGG + chr4_1:62506745-62506764 Msa0562560:CDS 45.0%
! TCGCTACATCTTTTGGACAG+TGG - chr4_1:62506894-62506913 None:intergenic 45.0%
CTGTCCAAAAGATGTAGCGA+TGG + chr4_1:62506894-62506913 Msa0562560:intron 45.0%
! CAACCCATCGCTACATCTTT+TGG - chr4_1:62506901-62506920 None:intergenic 45.0%
CAAAAGATGTAGCGATGGGT+TGG + chr4_1:62506899-62506918 Msa0562560:intron 45.0%
AAGACAATCAAGGGCTGCAA+TGG + chr4_1:62507095-62507114 Msa0562560:CDS 45.0%
GCAATTTGTGCTGCACTAAC+AGG + chr4_1:62507159-62507178 Msa0562560:CDS 45.0%
TGCTGCACTAACAGGTTTGA+TGG + chr4_1:62507167-62507186 Msa0562560:CDS 45.0%
CACTAACAGGTTTGATGGAG+AGG + chr4_1:62507172-62507191 Msa0562560:CDS 45.0%
TGATGAGACGGGATTCTCTT+TGG - chr4_1:62507224-62507243 None:intergenic 45.0%
GTGAAAGCTGCAATGGAGAA+TGG - chr4_1:62507768-62507787 None:intergenic 45.0%
GGATCAAGTGAAAGCTGCAA+TGG - chr4_1:62507775-62507794 None:intergenic 45.0%
GCAGTGGAAAGTTTATGAGG+AGG - chr4_1:62507824-62507843 None:intergenic 45.0%
GATAGGGTTCTTCAGATCAG+CGG - chr4_1:62507903-62507922 None:intergenic 45.0%
AATGAAGAAGGAGGAGGTGA+AGG - chr4_1:62507940-62507959 None:intergenic 45.0%
TCTTCCAGGAATGAAGAAGG+AGG - chr4_1:62507949-62507968 None:intergenic 45.0%
GGATCTTCCAGGAATGAAGA+AGG - chr4_1:62507952-62507971 None:intergenic 45.0%
! TGAATTTTCCGGGAAGGAAG+AGG + chr4_1:62508031-62508050 Msa0562560:three_prime_UTR 45.0%
TTCACTTTCCTCTTCCTTCC+CGG - chr4_1:62508042-62508061 None:intergenic 45.0%
GAGGAAAGTGAATTGGGGAA+TGG + chr4_1:62508050-62508069 Msa0562560:three_prime_UTR 45.0%
GGGAATGGAAGATCCTTGAA+TGG + chr4_1:62508065-62508084 Msa0562560:three_prime_UTR 45.0%
TGGTAAGAGGAGATGTGATG+AGG + chr4_1:62508249-62508268 Msa0562560:three_prime_UTR 45.0%
!!! TATTTTTATTTTTATTTTGT+TGG + chr4_1:62508229-62508248 Msa0562560:three_prime_UTR 5.0%
TTGGTTGCTGCATTACCGTG+AGG + chr4_1:62505608-62505627 Msa0562560:CDS 50.0%
TGGTTGCTGCATTACCGTGA+GGG + chr4_1:62505609-62505628 Msa0562560:CDS 50.0%
TTAAGATAGGTGGTCCGACG+TGG + chr4_1:62505871-62505890 Msa0562560:intron 50.0%
!! CTCCATTCATGGATTGGCCT+TGG + chr4_1:62506719-62506738 Msa0562560:CDS 50.0%
TGCCAAGGCCAATCCATGAA+TGG - chr4_1:62506724-62506743 None:intergenic 50.0%
AGACGGGATTCTCTTTGGTG+TGG - chr4_1:62507219-62507238 None:intergenic 50.0%
GAATGATCAATGGCATCGCG+TGG - chr4_1:62507853-62507872 None:intergenic 50.0%
ACCTCCTCCTTCTTCATTCC+TGG + chr4_1:62507942-62507961 Msa0562560:three_prime_UTR 50.0%
TCCAGGAATGAAGAAGGAGG+AGG - chr4_1:62507946-62507965 None:intergenic 50.0%
CGACGTATGGGATCCATTCA+AGG - chr4_1:62508081-62508100 None:intergenic 50.0%
TGAATGGATCCCATACGTCG+AGG + chr4_1:62508081-62508100 Msa0562560:three_prime_UTR 50.0%
GAATGGATCCCATACGTCGA+GGG + chr4_1:62508082-62508101 Msa0562560:three_prime_UTR 50.0%
TCCATTCTCCCTCGACGTAT+GGG - chr4_1:62508093-62508112 None:intergenic 50.0%
ATCCATTCTCCCTCGACGTA+TGG - chr4_1:62508094-62508113 None:intergenic 50.0%
! CCAAGTTTCTTTGGTGGCAG+AGG - chr4_1:62508137-62508156 None:intergenic 50.0%
CCTCTGCCACCAAAGAAACT+TGG + chr4_1:62508134-62508153 Msa0562560:three_prime_UTR 50.0%
GGTTGCTGCATTACCGTGAG+GGG + chr4_1:62505610-62505629 Msa0562560:CDS 55.0%
CATGTGTGCCTGGTACTGTG+TGG - chr4_1:62506583-62506602 None:intergenic 55.0%
ATGGCTCCATGGCACATAGC+TGG + chr4_1:62507114-62507133 Msa0562560:CDS 55.0%
CTACCACCAGCTATGTGCCA+TGG - chr4_1:62507123-62507142 None:intergenic 55.0%
CCTCACTGTGACTGTGCCTA+AGG - chr4_1:62507742-62507761 None:intergenic 55.0%
CCTTAGGCACAGTCACAGTG+AGG + chr4_1:62507739-62507758 Msa0562560:three_prime_UTR 55.0%
GTGTTCAAGGCGGATCTTCC+AGG - chr4_1:62507963-62507982 None:intergenic 55.0%
! AGAAGCGCACGTGTTCAAGG+CGG - chr4_1:62507973-62507992 None:intergenic 55.0%
! TCCAGAAGCGCACGTGTTCA+AGG - chr4_1:62507976-62507995 None:intergenic 55.0%
AGCATTTGCGAGCACACGTG+TGG - chr4_1:62508012-62508031 None:intergenic 55.0%
TCCCATACGTCGAGGGAGAA+TGG + chr4_1:62508089-62508108 Msa0562560:three_prime_UTR 55.0%
! TCTTTGGTGGCAGAGGCAGA+AGG - chr4_1:62508130-62508149 None:intergenic 55.0%
CAAGGGCTGCAATGGCTCCA+TGG + chr4_1:62507103-62507122 Msa0562560:CDS 60.0%
GCTCCATGGCACATAGCTGG+TGG + chr4_1:62507117-62507136 Msa0562560:CDS 60.0%
GCCTTGAACACGTGCGCTTC+TGG + chr4_1:62507972-62507991 Msa0562560:three_prime_UTR 60.0%
!! GAGCACACGTGTGGACTGGA+AGG - chr4_1:62508003-62508022 None:intergenic 60.0%
TTGCGAGCACACGTGTGGAC+TGG - chr4_1:62508007-62508026 None:intergenic 60.0%
CATCGCGTGGAGCGTAGCAG+TGG - chr4_1:62507840-62507859 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 62505444 62508352 62505444 ID=Msa0562560;Name=Msa0562560
chr4_1 mRNA 62505444 62508352 62505444 ID=Msa0562560-mRNA-1;Parent=Msa0562560;Name=Msa0562560-mRNA-1;_AED=0.05;_eAED=0.05;_QI=96|1|1|1|1|1|4|1029|214
chr4_1 exon 62505444 62505671 62505444 ID=Msa0562560-mRNA-1:exon:19764;Parent=Msa0562560-mRNA-1
chr4_1 exon 62505799 62505847 62505799 ID=Msa0562560-mRNA-1:exon:19765;Parent=Msa0562560-mRNA-1
chr4_1 exon 62506567 62506766 62506567 ID=Msa0562560-mRNA-1:exon:19766;Parent=Msa0562560-mRNA-1
chr4_1 exon 62507060 62508352 62507060 ID=Msa0562560-mRNA-1:exon:19767;Parent=Msa0562560-mRNA-1
chr4_1 five_prime_UTR 62505444 62505539 62505444 ID=Msa0562560-mRNA-1:five_prime_utr;Parent=Msa0562560-mRNA-1
chr4_1 CDS 62505540 62505671 62505540 ID=Msa0562560-mRNA-1:cds;Parent=Msa0562560-mRNA-1
chr4_1 CDS 62505799 62505847 62505799 ID=Msa0562560-mRNA-1:cds;Parent=Msa0562560-mRNA-1
chr4_1 CDS 62506567 62506766 62506567 ID=Msa0562560-mRNA-1:cds;Parent=Msa0562560-mRNA-1
chr4_1 CDS 62507060 62507323 62507060 ID=Msa0562560-mRNA-1:cds;Parent=Msa0562560-mRNA-1
chr4_1 three_prime_UTR 62507324 62508352 62507324 ID=Msa0562560-mRNA-1:three_prime_utr;Parent=Msa0562560-mRNA-1
Gene Sequence

>Msa0562560

ATGCGTTCTTATAGTACCTCAATTTTGGCTCACTTGTTTGGCATAATTGCCCTCATCCTTATGCTTGTTTGGTTGCTGCATTACCGTGAGGGGATTGAATATGACTCTGGAAATCCACTTCGTGTTTTCAATGTTCACCCTTTTCTGATGTTCTTTGGGTTCATTTTCCTTGTTGGTCAAGCAATAATGTCTTACCACACAGTACCAGGCACACATGAAACACAGAAGATTGTTCACATGACACTTCATTTCATTGCCATAGTTCTTGGAATTGTTGGTATATGTGCCGTTTTCAAGTTCCATGATATGGTGAACTTGGTTGATGTTTACAGTCTCCATTCATGGATTGGCCTTGGCACCTTTTGCTTGTTTGGCTTGCAGTGGCTTTTTGGACTGACATTCATGTTTCAAGGAACAAGACAATCAAGGGCTGCAATGGCTCCATGGCACATAGCTGGTGGTAGAGCACTATTTTTCATGGCAATTTGTGCTGCACTAACAGGTTTGATGGAGAGGTCTGCTATGTTAAAACTAATGCCACACCAAAGAGAATCCCGTCTCATCAATTTTACTGGTCTAGCAATTCTCTTGTTTGGAGTTTTTGTGGATATGACAGTTGGTTTGGCCCATATTGTTTATTCTTGA
Protein sequence

>Msa0562560

MRSYSTSILAHLFGIIALILMLVWLLHYREGIEYDSGNPLRVFNVHPFLMFFGFIFLVGQAIMSYHTVPGTHETQKIVHMTLHFIAIVLGIVGICAVFKFHDMVNLVDVYSLHSWIGLGTFCLFGLQWLFGLTFMFQGTRQSRAAMAPWHIAGGRALFFMAICAALTGLMERSAMLKLMPHQRESRLINFTGLAILLFGVFVDMTVGLAHIVYS*