AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0767810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGACAAATCGCTTGATTT+TGG 0.221118 5_2:+31720282 Msa0767810:five_prime_UTR
TTCTTTCTTAGCATCTTTCT+TGG 0.275859 5_2:-31720017 None:intergenic
GCTTGCTTCATTGAGTTAGA+AGG 0.322805 5_2:-31719935 None:intergenic
TGTGAATACAGTGTCTCTTC+TGG 0.330987 5_2:-31720079 None:intergenic
GAATGTGTTGAATAGGTTAA+TGG 0.333245 5_2:+31720159 Msa0767810:five_prime_UTR
TACAGTGTCTCTTCTGGCCT+CGG 0.375247 5_2:-31720073 None:intergenic
GGTAAGAAACAATTTCTTCA+TGG 0.376328 5_2:-31722608 None:intergenic
GGGATCGGAGAAGGAAGAAT+TGG 0.406847 5_2:+31720192 Msa0767810:five_prime_UTR
GGCTGACACGGATTGAGATT+CGG 0.451832 5_2:-31719890 None:intergenic
TAGATATGAATGTGTTGAAT+AGG 0.453928 5_2:+31720152 Msa0767810:five_prime_UTR
TGAGTCTGCAGCGGCGGCGG+CGG 0.455416 5_2:-31719966 None:intergenic
TGAACATACTTCCATTCTTG+TGG 0.480056 5_2:+31722635 Msa0767810:CDS
TGATGAGTCTGCAGCGGCGG+CGG 0.481622 5_2:-31719969 None:intergenic
GGGGAATGTAGTTATCAAAT+GGG 0.485794 5_2:+31720118 Msa0767810:five_prime_UTR
CAAGAATGGAAGTATGTTCA+AGG 0.487778 5_2:-31722632 None:intergenic
CATCAAACCGGCCGCGTAAG+AGG 0.488792 5_2:+31719987 Msa0767810:five_prime_UTR
AGAAGGAAGAATTGGAAAGA+AGG 0.489922 5_2:+31720200 Msa0767810:five_prime_UTR
TCAAGAGTTGAAACTGTTTG+TGG 0.491313 5_2:+31720240 Msa0767810:five_prime_UTR
AATGGAATCGATTGAGGGAT+CGG 0.493309 5_2:+31720177 Msa0767810:five_prime_UTR
GGCTTTATAGTCCACAAGAA+TGG 0.495039 5_2:-31722646 None:intergenic
CTTGCTTCATTGAGTTAGAA+GGG 0.508767 5_2:-31719934 None:intergenic
AGGAAGAATTGGAAAGAAGG+TGG 0.531844 5_2:+31720203 Msa0767810:five_prime_UTR
CCGCTGCAGACTCATCAAAC+CGG 0.534109 5_2:+31719975 Msa0767810:five_prime_UTR
ATCGATTGAGGGATCGGAGA+AGG 0.536386 5_2:+31720183 Msa0767810:five_prime_UTR
TAGGTTAATGGAATCGATTG+AGG 0.553633 5_2:+31720171 Msa0767810:five_prime_UTR
TAAAGCGCCTCTTACGCGGC+CGG 0.553884 5_2:-31719994 None:intergenic
AGGTTAATGGAATCGATTGA+GGG 0.560272 5_2:+31720172 Msa0767810:five_prime_UTR
AGAAGAGACACTGTATTCAC+AGG 0.572182 5_2:+31720081 Msa0767810:five_prime_UTR
CACAGGAAGAATTTGTGAAG+GGG 0.572656 5_2:+31720098 Msa0767810:five_prime_UTR
GTTTGATGAGTCTGCAGCGG+CGG 0.580694 5_2:-31719972 None:intergenic
TTCACAGGAAGAATTTGTGA+AGG 0.593335 5_2:+31720096 Msa0767810:five_prime_UTR
TCACAGGAAGAATTTGTGAA+GGG 0.599562 5_2:+31720097 Msa0767810:five_prime_UTR
AGAGTTGAAACTGTTTGTGG+AGG 0.602889 5_2:+31720243 Msa0767810:five_prime_UTR
TGCTTCATTGAGTTAGAAGG+GGG 0.603394 5_2:-31719932 None:intergenic
CGTCTTAGACACGAAGCATG+AGG 0.614770 5_2:+31720320 Msa0767810:exon
ACAGGAAGAATTTGTGAAGG+GGG 0.620371 5_2:+31720099 Msa0767810:five_prime_UTR
TTGCTTCATTGAGTTAGAAG+GGG 0.637305 5_2:-31719933 None:intergenic
TTGGTAAAGCGCCTCTTACG+CGG 0.641927 5_2:-31719998 None:intergenic
CCGGTTTGATGAGTCTGCAG+CGG 0.651993 5_2:-31719975 None:intergenic
AAAATGCTGCTAAGAAACCG+AGG 0.699686 5_2:+31720056 Msa0767810:five_prime_UTR
GAATGGAAGTATGTTCAAGG+AGG 0.714543 5_2:-31722629 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTGTTATGTATTAT+TGG + chr5_2:31722049-31722068 Msa0767810:intron 10.0%
!!! TATTTTTGTTATGTATTATT+GGG + chr5_2:31722050-31722069 Msa0767810:intron 10.0%
!!! TTAGATTTATACTTATTTTG+TGG + chr5_2:31720393-31720412 Msa0767810:intron 15.0%
!! AACAAGAAAAGTTAATAATA+AGG - chr5_2:31721295-31721314 None:intergenic 15.0%
!!! ATTAACTTTTCTTGTTTATA+TGG + chr5_2:31721299-31721318 Msa0767810:intron 15.0%
!!! AAAACATGTTGATTTATTAA+AGG + chr5_2:31721495-31721514 Msa0767810:intron 15.0%
!!! CATTTATATCTTTTTAAATC+TGG + chr5_2:31721795-31721814 Msa0767810:intron 15.0%
!! TAATATAAGCTGATACTTTA+TGG + chr5_2:31720370-31720389 Msa0767810:intron 20.0%
!!! ACATTATTGATTTATCTCTT+TGG + chr5_2:31720989-31721008 Msa0767810:intron 20.0%
!! GAAATAGTAAAGTAATGTAA+AGG + chr5_2:31721077-31721096 Msa0767810:intron 20.0%
!!! GTCTACTTTTTTTGTTTATA+TGG + chr5_2:31721334-31721353 Msa0767810:intron 20.0%
!!! TATATCAGTTTTATGTAATC+TGG + chr5_2:31721637-31721656 Msa0767810:intron 20.0%
!!! ATTTTAGAATGAATCCTTTA+CGG + chr5_2:31721705-31721724 Msa0767810:intron 20.0%
!! ATTCATAGCAAACTATATTA+CGG + chr5_2:31721887-31721906 Msa0767810:intron 20.0%
!!! TATACCAAAGAGTTTTTTTT+GGG + chr5_2:31722073-31722092 Msa0767810:intron 20.0%
!! TTCATATAATCATAGTTATG+AGG + chr5_2:31722436-31722455 Msa0767810:intron 20.0%
! TAGATATGAATGTGTTGAAT+AGG + chr5_2:31720152-31720171 Msa0767810:five_prime_UTR 25.0%
!!! TGAACATGTTTTGTTTGATA+TGG + chr5_2:31720677-31720696 Msa0767810:intron 25.0%
! TTGATATGAACATAAGAATG+TGG + chr5_2:31720726-31720745 Msa0767810:intron 25.0%
!! TTAGTGAAACTTTACCTTTT+AGG + chr5_2:31720761-31720780 Msa0767810:intron 25.0%
!! TAGTGAAACTTTACCTTTTA+GGG + chr5_2:31720762-31720781 Msa0767810:intron 25.0%
!! GATTTATCTCTTTGGAAATT+AGG + chr5_2:31720997-31721016 Msa0767810:intron 25.0%
! ATGTAAAGGATTAGACATAA+AGG + chr5_2:31721091-31721110 Msa0767810:intron 25.0%
! ATAATCAAGATAACTGATTG+TGG + chr5_2:31721251-31721270 Msa0767810:intron 25.0%
! GTTTATATGGTTGTATGAAT+TGG + chr5_2:31721312-31721331 Msa0767810:intron 25.0%
!! AAGTGAAATGTTTTGTCAAT+TGG - chr5_2:31721386-31721405 None:intergenic 25.0%
! ATATTGCGTAAAAACTATGT+TGG - chr5_2:31721449-31721468 None:intergenic 25.0%
!! ACATAGTTTTTACGCAATAT+TGG + chr5_2:31721449-31721468 Msa0767810:intron 25.0%
!!! TTTCTTTTCAATCTGTTTCT+GGG + chr5_2:31721518-31721537 Msa0767810:intron 25.0%
! GACTGCATTACTTAATTATT+TGG + chr5_2:31721540-31721559 Msa0767810:intron 25.0%
! TATAACAATCTACGACTATT+TGG - chr5_2:31721621-31721640 None:intergenic 25.0%
! AAAAGAACCTGTTATTAAGA+AGG - chr5_2:31721771-31721790 None:intergenic 25.0%
!! TCTTAATAACAGGTTCTTTT+AGG + chr5_2:31721771-31721790 Msa0767810:intron 25.0%
!! CTTAATAACAGGTTCTTTTA+GGG + chr5_2:31721772-31721791 Msa0767810:intron 25.0%
!! CTTTTTAAATCTGGATTGAT+AGG + chr5_2:31721804-31721823 Msa0767810:intron 25.0%
! TAAATGTATGCTAAGTTTAC+TGG + chr5_2:31721836-31721855 Msa0767810:intron 25.0%
! TTCATAGCAAACTATATTAC+GGG + chr5_2:31721888-31721907 Msa0767810:intron 25.0%
! AACTATATTACGGGTTTAAA+AGG + chr5_2:31721897-31721916 Msa0767810:intron 25.0%
!!! GTATACCAAAGAGTTTTTTT+TGG + chr5_2:31722072-31722091 Msa0767810:intron 25.0%
! TGTGTTAATTGTATTCTTGT+TGG + chr5_2:31722108-31722127 Msa0767810:intron 25.0%
!!! AATTGTATTCTTGTTGGTTT+TGG + chr5_2:31722114-31722133 Msa0767810:intron 25.0%
! ACAATTTGTAGAACAGATTA+AGG - chr5_2:31722149-31722168 None:intergenic 25.0%
!!! TGGTAATTTTTGGAAGATTT+TGG + chr5_2:31722269-31722288 Msa0767810:intron 25.0%
!! TGTGGTACAACTTAAATTTT+GGG + chr5_2:31722388-31722407 Msa0767810:intron 25.0%
!!! TACAACTTAAATTTTGGGTT+GGG + chr5_2:31722393-31722412 Msa0767810:intron 25.0%
! TATAATCATAGTTATGAGGT+TGG + chr5_2:31722440-31722459 Msa0767810:intron 25.0%
! AACCACACATAAAAAATGAT+AGG - chr5_2:31722670-31722689 None:intergenic 25.0%
TTCTTTCTTAGCATCTTTCT+TGG - chr5_2:31720020-31720039 None:intergenic 30.0%
GAATGTGTTGAATAGGTTAA+TGG + chr5_2:31720159-31720178 Msa0767810:five_prime_UTR 30.0%
!!! TGGTGTTTTGATGATTGTAA+TGG + chr5_2:31720513-31720532 Msa0767810:intron 30.0%
TGTTATAAGACCACAATCAT+TGG + chr5_2:31720560-31720579 Msa0767810:intron 30.0%
!! ATATTTTGAGCCAATGATTG+TGG - chr5_2:31720573-31720592 None:intergenic 30.0%
TTGGCTCAAAATATGCAATT+CGG + chr5_2:31720579-31720598 Msa0767810:intron 30.0%
! CAAAATATGCAATTCGGTTT+TGG + chr5_2:31720585-31720604 Msa0767810:intron 30.0%
TTAGAGAAACTGTATAGAAG+TGG - chr5_2:31720919-31720938 None:intergenic 30.0%
ATCATAAGTTGTTCAGTTGT+AGG + chr5_2:31720940-31720959 Msa0767810:intron 30.0%
TCATAAGTTGTTCAGTTGTA+GGG + chr5_2:31720941-31720960 Msa0767810:intron 30.0%
GTGAATGGGATGTATAAATA+GGG + chr5_2:31721042-31721061 Msa0767810:intron 30.0%
TGAATGGGATGTATAAATAG+GGG + chr5_2:31721043-31721062 Msa0767810:intron 30.0%
TAAAGGAAAGAGAGATTACA+AGG + chr5_2:31721108-31721127 Msa0767810:intron 30.0%
AAAGGAAAGAGAGATTACAA+GGG + chr5_2:31721109-31721128 Msa0767810:intron 30.0%
! TTTGTTCATGTTCCTTTTAG+AGG + chr5_2:31721179-31721198 Msa0767810:intron 30.0%
! GTTTCTTTTCAATCTGTTTC+TGG + chr5_2:31721517-31721536 Msa0767810:intron 30.0%
! TATTTGGTTCCTTGCATATT+AGG - chr5_2:31721605-31721624 None:intergenic 30.0%
CTTAAGAACGAAGATGAAAA+AGG - chr5_2:31721682-31721701 None:intergenic 30.0%
GTATCTTCCTTCTTAATAAC+AGG + chr5_2:31721761-31721780 Msa0767810:intron 30.0%
!!! TTTTGGTGCTTGGTAATTTT+TGG + chr5_2:31722259-31722278 Msa0767810:intron 30.0%
! CTGTGGTACAACTTAAATTT+TGG + chr5_2:31722387-31722406 Msa0767810:intron 30.0%
!! GTACAACTTAAATTTTGGGT+TGG + chr5_2:31722392-31722411 Msa0767810:intron 30.0%
!!! ACAACTTAAATTTTGGGTTG+GGG + chr5_2:31722394-31722413 Msa0767810:intron 30.0%
!! AAAACTTTGGCAGTTTTATC+AGG - chr5_2:31722523-31722542 None:intergenic 30.0%
!!! AAACTGCCAAAGTTTTGAAA+TGG + chr5_2:31722527-31722546 Msa0767810:intron 30.0%
GGTAAGAAACAATTTCTTCA+TGG - chr5_2:31722611-31722630 None:intergenic 30.0%
!!! AGCCTATCATTTTTTATGTG+TGG + chr5_2:31722665-31722684 Msa0767810:CDS 30.0%
!!! TCATTTTTTATGTGTGGTTC+AGG + chr5_2:31722671-31722690 Msa0767810:CDS 30.0%
! ATGAATACTGCAAGTTTTCA+AGG - chr5_2:31722713-31722732 None:intergenic 30.0%
!!! TCGATATTTTTTGGCTGACA+CGG - chr5_2:31719905-31719924 None:intergenic 35.0%
!!! GGAGTTGGTTCGATATTTTT+TGG - chr5_2:31719914-31719933 None:intergenic 35.0%
TTGCTTCATTGAGTTAGAAG+GGG - chr5_2:31719936-31719955 None:intergenic 35.0%
CTTGCTTCATTGAGTTAGAA+GGG - chr5_2:31719937-31719956 None:intergenic 35.0%
GAAAGAAAAATCACCCAACA+AGG + chr5_2:31720033-31720052 Msa0767810:five_prime_UTR 35.0%
! TTAGCAGCATTTTCCTTGTT+GGG - chr5_2:31720049-31720068 None:intergenic 35.0%
TTCACAGGAAGAATTTGTGA+AGG + chr5_2:31720096-31720115 Msa0767810:five_prime_UTR 35.0%
TCACAGGAAGAATTTGTGAA+GGG + chr5_2:31720097-31720116 Msa0767810:five_prime_UTR 35.0%
GGGGAATGTAGTTATCAAAT+GGG + chr5_2:31720118-31720137 Msa0767810:five_prime_UTR 35.0%
TAGGTTAATGGAATCGATTG+AGG + chr5_2:31720171-31720190 Msa0767810:five_prime_UTR 35.0%
AGGTTAATGGAATCGATTGA+GGG + chr5_2:31720172-31720191 Msa0767810:five_prime_UTR 35.0%
AGAAGGAAGAATTGGAAAGA+AGG + chr5_2:31720200-31720219 Msa0767810:five_prime_UTR 35.0%
TCAAGAGTTGAAACTGTTTG+TGG + chr5_2:31720240-31720259 Msa0767810:five_prime_UTR 35.0%
! AGAGACAAATCGCTTGATTT+TGG + chr5_2:31720282-31720301 Msa0767810:five_prime_UTR 35.0%
!!! GGTTTGGGAAGTTTTTTGTA+TGG + chr5_2:31720341-31720360 Msa0767810:intron 35.0%
CAAATGCAATGCATAGATTG+TGG + chr5_2:31720489-31720508 Msa0767810:intron 35.0%
!! ATTGTGATGCTTGTTGTGTT+AGG + chr5_2:31720639-31720658 Msa0767810:intron 35.0%
!! TTGTGATGCTTGTTGTGTTA+GGG + chr5_2:31720640-31720659 Msa0767810:intron 35.0%
! CTTGTTGTGTTAGGGATATT+GGG + chr5_2:31720648-31720667 Msa0767810:intron 35.0%
! GATATGGTTCGTTTGTTAGT+TGG + chr5_2:31720693-31720712 Msa0767810:intron 35.0%
CAAGTGACATTATCCCTAAA+AGG - chr5_2:31720778-31720797 None:intergenic 35.0%
TGCTGAAATTGAATAGCACT+AGG + chr5_2:31720883-31720902 Msa0767810:intron 35.0%
AAATCAATAATGTCTCCACC+TGG - chr5_2:31720982-31721001 None:intergenic 35.0%
! TTTTTGTGACAAGAAGAGAG+AGG + chr5_2:31721020-31721039 Msa0767810:intron 35.0%
GGTGAATGGGATGTATAAAT+AGG + chr5_2:31721041-31721060 Msa0767810:intron 35.0%
GAATGGGATGTATAAATAGG+GGG + chr5_2:31721044-31721063 Msa0767810:intron 35.0%
AAAGAGAGATTACAAGGGTT+AGG + chr5_2:31721114-31721133 Msa0767810:intron 35.0%
AAGAGAGATTACAAGGGTTA+GGG + chr5_2:31721115-31721134 Msa0767810:intron 35.0%
GGTAGTATGGTGAATAAGAT+GGG + chr5_2:31721136-31721155 Msa0767810:intron 35.0%
TACTTGAATGCTCCTCTAAA+AGG - chr5_2:31721194-31721213 None:intergenic 35.0%
!! TTTAGAGGAGCATTCAAGTA+TGG + chr5_2:31721194-31721213 Msa0767810:intron 35.0%
TTGCGTAAAAACTATGTTGG+AGG - chr5_2:31721446-31721465 None:intergenic 35.0%
TTTGTGTTGCCTAATATGCA+AGG + chr5_2:31721593-31721612 Msa0767810:intron 35.0%
! GAAAAAGGCACATTCATTTG+TGG - chr5_2:31721667-31721686 None:intergenic 35.0%
GTCATACAAAAGGTCTCATT+TGG - chr5_2:31722005-31722024 None:intergenic 35.0%
! ATGAGACCTTTTGTATGACA+CGG + chr5_2:31722006-31722025 Msa0767810:intron 35.0%
TTAAGACCGTGTCATACAAA+AGG - chr5_2:31722015-31722034 None:intergenic 35.0%
ACAGCCCAAAAAAAACTCTT+TGG - chr5_2:31722080-31722099 None:intergenic 35.0%
!!! TGTATTCTTGTTGGTTTTGG+AGG + chr5_2:31722117-31722136 Msa0767810:intron 35.0%
! TGATTTGTAGTGTGATAGCT+AGG + chr5_2:31722191-31722210 Msa0767810:intron 35.0%
!!! TTTTGGAAGATTTTGGCTTC+AGG + chr5_2:31722276-31722295 Msa0767810:intron 35.0%
!!! TTTGGAAGATTTTGGCTTCA+GGG + chr5_2:31722277-31722296 Msa0767810:intron 35.0%
! TTAAGTTGTACCACAGTTAC+AGG - chr5_2:31722383-31722402 None:intergenic 35.0%
AGAAATACAACCAACTGCAA+GGG - chr5_2:31722497-31722516 None:intergenic 35.0%
AAGAAATACAACCAACTGCA+AGG - chr5_2:31722498-31722517 None:intergenic 35.0%
CATTCGCCATTTCAAAACTT+TGG - chr5_2:31722536-31722555 None:intergenic 35.0%
!!! CAAAGTTTTGAAATGGCGAA+TGG + chr5_2:31722534-31722553 Msa0767810:intron 35.0%
! CAAGAATGGAAGTATGTTCA+AGG - chr5_2:31722635-31722654 None:intergenic 35.0%
TGAACATACTTCCATTCTTG+TGG + chr5_2:31722635-31722654 Msa0767810:CDS 35.0%
!! TACTGCAAGTTTTCAAGGAA+GGG - chr5_2:31722708-31722727 None:intergenic 35.0%
!! ATACTGCAAGTTTTCAAGGA+AGG - chr5_2:31722709-31722728 None:intergenic 35.0%
TGCTTCATTGAGTTAGAAGG+GGG - chr5_2:31719935-31719954 None:intergenic 40.0%
GCTTGCTTCATTGAGTTAGA+AGG - chr5_2:31719938-31719957 None:intergenic 40.0%
! CTTAGCAGCATTTTCCTTGT+TGG - chr5_2:31720050-31720069 None:intergenic 40.0%
AAAATGCTGCTAAGAAACCG+AGG + chr5_2:31720056-31720075 Msa0767810:five_prime_UTR 40.0%
TGTGAATACAGTGTCTCTTC+TGG - chr5_2:31720082-31720101 None:intergenic 40.0%
AGAAGAGACACTGTATTCAC+AGG + chr5_2:31720081-31720100 Msa0767810:five_prime_UTR 40.0%
CACAGGAAGAATTTGTGAAG+GGG + chr5_2:31720098-31720117 Msa0767810:five_prime_UTR 40.0%
ACAGGAAGAATTTGTGAAGG+GGG + chr5_2:31720099-31720118 Msa0767810:five_prime_UTR 40.0%
GGGGGAATGTAGTTATCAAA+TGG + chr5_2:31720117-31720136 Msa0767810:five_prime_UTR 40.0%
AATGGAATCGATTGAGGGAT+CGG + chr5_2:31720177-31720196 Msa0767810:five_prime_UTR 40.0%
AGGAAGAATTGGAAAGAAGG+TGG + chr5_2:31720203-31720222 Msa0767810:five_prime_UTR 40.0%
AGAGTTGAAACTGTTTGTGG+AGG + chr5_2:31720243-31720262 Msa0767810:five_prime_UTR 40.0%
! GACAAATCGCTTGATTTTGG+AGG + chr5_2:31720285-31720304 Msa0767810:five_prime_UTR 40.0%
TGCAATGCATAGATTGTGGA+TGG + chr5_2:31720493-31720512 Msa0767810:intron 40.0%
!! GCTTGTTGTGTTAGGGATAT+TGG + chr5_2:31720647-31720666 Msa0767810:intron 40.0%
ACAAGAAGAGAGAGGTGAAT+GGG + chr5_2:31721028-31721047 Msa0767810:intron 40.0%
TTACAAGGGTTAGGGTAGTA+TGG + chr5_2:31721123-31721142 Msa0767810:intron 40.0%
GGGTAGTATGGTGAATAAGA+TGG + chr5_2:31721135-31721154 Msa0767810:intron 40.0%
CGTAAAAACTATGTTGGAGG+AGG - chr5_2:31721443-31721462 None:intergenic 40.0%
CTGTCACAGATAAACCGTAA+AGG - chr5_2:31721722-31721741 None:intergenic 40.0%
CTTTACGGTTTATCTGTGAC+AGG + chr5_2:31721720-31721739 Msa0767810:intron 40.0%
! TCTGTGACAGGTTCTGTTTT+AGG + chr5_2:31721732-31721751 Msa0767810:intron 40.0%
! CTGTGACAGGTTCTGTTTTA+GGG + chr5_2:31721733-31721752 Msa0767810:intron 40.0%
GTATGCTAAGTTTACTGGAC+TGG + chr5_2:31721841-31721860 Msa0767810:intron 40.0%
!!! TTGGAAGATTTTGGCTTCAG+GGG + chr5_2:31722278-31722297 Msa0767810:intron 40.0%
!!! ATTTTGGCTTCAGGGGATAT+TGG + chr5_2:31722285-31722304 Msa0767810:intron 40.0%
TAAGCTGTAACCTGTAACTG+TGG + chr5_2:31722370-31722389 Msa0767810:intron 40.0%
AAACCAAAAACCCTTGCAGT+TGG + chr5_2:31722484-31722503 Msa0767810:intron 40.0%
! GAATGGAAGTATGTTCAAGG+AGG - chr5_2:31722632-31722651 None:intergenic 40.0%
! GGCTTTATAGTCCACAAGAA+TGG - chr5_2:31722649-31722668 None:intergenic 40.0%
ATTGAGTTAGAAGGGGGAGT+TGG - chr5_2:31719929-31719948 None:intergenic 45.0%
!! ATCGCTTGATTTTGGAGGCA+TGG + chr5_2:31720290-31720309 Msa0767810:five_prime_UTR 45.0%
TAGACACGAAGCATGAGGTT+TGG + chr5_2:31720325-31720344 Msa0767810:intron 45.0%
AGACACGAAGCATGAGGTTT+GGG + chr5_2:31720326-31720345 Msa0767810:intron 45.0%
!! CTCCATTTTGATGAACCAGG+TGG + chr5_2:31720964-31720983 Msa0767810:intron 45.0%
CTCCACCTGGTTCATCAAAA+TGG - chr5_2:31720969-31720988 None:intergenic 45.0%
GACAAGAAGAGAGAGGTGAA+TGG + chr5_2:31721027-31721046 Msa0767810:intron 45.0%
! AGGAGGATCTTTTCGCAATG+GGG - chr5_2:31721426-31721445 None:intergenic 45.0%
! GAGGAGGATCTTTTCGCAAT+GGG - chr5_2:31721427-31721446 None:intergenic 45.0%
! TGCTAAGTTTACTGGACTGG+TGG + chr5_2:31721844-31721863 Msa0767810:intron 45.0%
!! TTACTGGACTGGTGGTCTTA+TGG + chr5_2:31721852-31721871 Msa0767810:intron 45.0%
AATGATCTGTGCAATCGTGC+AGG + chr5_2:31722224-31722243 Msa0767810:intron 45.0%
GGGGATATTGGCATTAGTGT+CGG + chr5_2:31722297-31722316 Msa0767810:intron 45.0%
GGGATATTGGCATTAGTGTC+GGG + chr5_2:31722298-31722317 Msa0767810:intron 45.0%
! CAACCAACTGCAAGGGTTTT+TGG - chr5_2:31722490-31722509 None:intergenic 45.0%
! GGCTGACACGGATTGAGATT+CGG - chr5_2:31719893-31719912 None:intergenic 50.0%
! TTGGTAAAGCGCCTCTTACG+CGG - chr5_2:31720001-31720020 None:intergenic 50.0%
TACAGTGTCTCTTCTGGCCT+CGG - chr5_2:31720076-31720095 None:intergenic 50.0%
ATCGATTGAGGGATCGGAGA+AGG + chr5_2:31720183-31720202 Msa0767810:five_prime_UTR 50.0%
GGGATCGGAGAAGGAAGAAT+TGG + chr5_2:31720192-31720211 Msa0767810:five_prime_UTR 50.0%
!! TTGATTTTGGAGGCATGGCG+AGG + chr5_2:31720295-31720314 Msa0767810:five_prime_UTR 50.0%
CGTCTTAGACACGAAGCATG+AGG + chr5_2:31720320-31720339 Msa0767810:exon 50.0%
!! GGGCTCCATTTTGATGAACC+AGG + chr5_2:31720961-31720980 Msa0767810:intron 50.0%
! GGAGGAGGATCTTTTCGCAA+TGG - chr5_2:31721428-31721447 None:intergenic 50.0%
!!! GCAGGTTCTGTGCTGGTTTT+TGG + chr5_2:31722242-31722261 Msa0767810:intron 50.0%
!!! CTGTGCTGGTTTTTGGTGCT+TGG + chr5_2:31722249-31722268 Msa0767810:intron 50.0%
GTTTGATGAGTCTGCAGCGG+CGG - chr5_2:31719975-31719994 None:intergenic 55.0%
! CCGGTTTGATGAGTCTGCAG+CGG - chr5_2:31719978-31719997 None:intergenic 55.0%
CCGCTGCAGACTCATCAAAC+CGG + chr5_2:31719975-31719994 Msa0767810:five_prime_UTR 55.0%
CAATCGTGCAGGTTCTGTGC+TGG + chr5_2:31722235-31722254 Msa0767810:intron 55.0%
CATCAAACCGGCCGCGTAAG+AGG + chr5_2:31719987-31720006 Msa0767810:five_prime_UTR 60.0%
! TAAAGCGCCTCTTACGCGGC+CGG - chr5_2:31719997-31720016 None:intergenic 60.0%
TGATGAGTCTGCAGCGGCGG+CGG - chr5_2:31719972-31719991 None:intergenic 65.0%
TGAGTCTGCAGCGGCGGCGG+CGG - chr5_2:31719969-31719988 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 31719884 31722737 31719884 ID=Msa0767810;Name=Msa0767810
chr5_2 mRNA 31719884 31722737 31719884 ID=Msa0767810-mRNA-1;Parent=Msa0767810;Name=Msa0767810-mRNA-1;_AED=0.28;_eAED=0.43;_QI=453|0|0.25|0.75|1|1|4|0|87
chr5_2 exon 31719884 31720341 31719884 ID=Msa0767810-mRNA-1:exon:11629;Parent=Msa0767810-mRNA-1
chr5_2 exon 31721361 31721453 31721361 ID=Msa0767810-mRNA-1:exon:11630;Parent=Msa0767810-mRNA-1
chr5_2 exon 31722363 31722368 31722363 ID=Msa0767810-mRNA-1:exon:11631;Parent=Msa0767810-mRNA-1
chr5_2 exon 31722578 31722737 31722578 ID=Msa0767810-mRNA-1:exon:11632;Parent=Msa0767810-mRNA-1
chr5_2 five_prime_UTR 31719884 31720336 31719884 ID=Msa0767810-mRNA-1:five_prime_utr;Parent=Msa0767810-mRNA-1
chr5_2 CDS 31720337 31720341 31720337 ID=Msa0767810-mRNA-1:cds;Parent=Msa0767810-mRNA-1
chr5_2 CDS 31721361 31721453 31721361 ID=Msa0767810-mRNA-1:cds;Parent=Msa0767810-mRNA-1
chr5_2 CDS 31722363 31722368 31722363 ID=Msa0767810-mRNA-1:cds;Parent=Msa0767810-mRNA-1
chr5_2 CDS 31722578 31722737 31722578 ID=Msa0767810-mRNA-1:cds;Parent=Msa0767810-mRNA-1
Gene Sequence

>Msa0767810

ATGAGTTTGCTTGCTTTCACAATAAAACCAATTGACAAAACATTTCACTTGTCTACACAAAGTGATTCCCCATTGCGAAAAGATCCTCCTCCAACATATAAGTTATTTCGAGATGCTGCTTTATTTTCTTCTAACCATGAAGAAATTGTTTCTTACCTCCTTGAACATACTTCCATTCTTGTGGACTATAAAGCCTATCATTTTTTATGTGTGGTTCAGGAACAGTTTGAGCCCTTCCTTGAAAACTTGCAGTATTCATTGTGA
Protein sequence

>Msa0767810

MSLLAFTIKPIDKTFHLSTQSDSPLRKDPPPTYKLFRDAALFSSNHEEIVSYLLEHTSILVDYKAYHFLCVVQEQFEPFLENLQYSL*