AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1306760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 494 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAATGAAGAACATAAAA+TGG 0.254405 8_4:+1731747 Msa1306760:five_prime_UTR
GAATGGAAACGGATGGTTTC+AGG 0.257335 8_4:-1724101 None:intergenic
CGACCAGATCTACATCTTTC+CGG 0.264446 8_4:+1724169 Msa1306760:five_prime_UTR
CCGGCTTCAGAGGTCATTTC+CGG 0.310570 8_4:+1724414 Msa1306760:five_prime_UTR
CAGCACTTCAACTACTTGTT+GGG 0.337129 8_4:+1732846 Msa1306760:intron
TGATATTTCCGGCCACCATT+TGG 0.350933 8_4:+1724304 Msa1306760:five_prime_UTR
ATGTAGATCTGGTCGGAAAT+TGG 0.359349 8_4:-1724161 None:intergenic
AATATATGGTTTCAAAAGGT+TGG 0.371174 8_4:+1733151 Msa1306760:three_prime_UTR
GAATGGGGAGGTTAGTTTAG+TGG 0.390593 8_4:-1724338 None:intergenic
TTGGCAGAATAGAGGATTGT+TGG 0.410840 8_4:+1731692 Msa1306760:intron
GAAAGATCAATAGGATAAAT+CGG 0.416718 8_4:-1724385 None:intergenic
GCAGCACTTCAACTACTTGT+TGG 0.416835 8_4:+1732845 Msa1306760:intron
TTGGCGGTGACGGTGGTTCA+TGG 0.428592 8_4:-1724142 None:intergenic
CTTCAACTTTCAGTTGCAAA+AGG 0.428853 8_4:+1732288 Msa1306760:CDS
TTTGAATATATGGTTTCAAA+AGG 0.435804 8_4:+1733147 Msa1306760:three_prime_UTR
GGTTGTCCGCGGTGGTTGGC+CGG 0.442271 8_4:-1724433 None:intergenic
GGGAAATATGTGAGAGATTG+TGG 0.445710 8_4:-1724064 None:intergenic
GTTTCAGGCGAGAGAGAGAA+AGG 0.446764 8_4:-1724086 None:intergenic
AGATCATCAACCTCTCTTCT+TGG 0.447330 8_4:+1724490 Msa1306760:five_prime_UTR
CGGCGGTCTGAAAGATCAAT+AGG 0.452640 8_4:-1724394 None:intergenic
GGAGGTTAGTTTAGTGGTGG+TGG 0.456781 8_4:-1724332 None:intergenic
CTAATTTGAGAGAAAGATAG+AGG 0.462425 8_4:-1724238 None:intergenic
GAGAAAGATAGAGGAGAATG+AGG 0.462572 8_4:-1724229 None:intergenic
AAATAAAATTGAAGAGAAGA+TGG 0.475496 8_4:+1732898 Msa1306760:CDS
GAGAGATTGTGGAAACTGAT+AGG 0.481171 8_4:-1724053 None:intergenic
GAAAACCTAGCAAATAAAAG+TGG 0.503691 8_4:+1732369 Msa1306760:CDS
CGGTGGTTGTCCGCGGTGGT+TGG 0.504633 8_4:-1724437 None:intergenic
ATCTTGTGAAGATTTCTGCT+TGG 0.511495 8_4:+1733085 Msa1306760:three_prime_UTR
GAGGATTGTTGGCTGGAGGC+AGG 0.512272 8_4:+1731703 Msa1306760:five_prime_UTR
CAGAATAGAGGATTGTTGGC+TGG 0.514300 8_4:+1731696 Msa1306760:intron
TCGCCGGAAAGATGTAGATC+TGG 0.515851 8_4:-1724172 None:intergenic
TGGTCGGAAATTGGCGGTGA+CGG 0.521702 8_4:-1724152 None:intergenic
CTATTGATCTTTCAGACCGC+CGG 0.524979 8_4:+1724395 Msa1306760:five_prime_UTR
CCGGAAATGACCTCTGAAGC+CGG 0.528005 8_4:-1724414 None:intergenic
TTTCAGGCGAGAGAGAGAAA+GGG 0.536377 8_4:-1724085 None:intergenic
ACCGCGGACAACCACCGTAC+CGG 0.544500 8_4:+1724443 Msa1306760:five_prime_UTR
TTTGCAACTGAAAGTTGAAG+TGG 0.554271 8_4:-1732285 None:intergenic
ATAAATAAATTACTCCGGTA+CGG 0.556420 8_4:-1724457 None:intergenic
AGAAATGATGAGGAAGATCA+AGG 0.559760 8_4:-1733007 None:intergenic
GTGGTGGTGGTGCATCCAAA+TGG 0.564680 8_4:-1724319 None:intergenic
TTTCAGACCGCCGGCTTCAG+AGG 0.565037 8_4:+1724404 Msa1306760:five_prime_UTR
GTGAGGGAAGAGAAATGATG+AGG 0.581781 8_4:-1733017 None:intergenic
AATGAATTTACCAAGAAGAG+AGG 0.588492 8_4:-1724500 None:intergenic
TTCAGGCGAGAGAGAGAAAG+GGG 0.588717 8_4:-1724084 None:intergenic
TGGGGAGGTTAGTTTAGTGG+TGG 0.601967 8_4:-1724335 None:intergenic
TCCGGTACGGTGGTTGTCCG+CGG 0.602090 8_4:-1724444 None:intergenic
AAGATATGAATGGAAACGGA+TGG 0.606963 8_4:-1724108 None:intergenic
TGGTGCATCCAAATGGTGGC+CGG 0.613784 8_4:-1724312 None:intergenic
TACAAAATAGAAGTGAAGAG+TGG 0.617847 8_4:+1732942 Msa1306760:CDS
TAGATCTGGTCGGAAATTGG+CGG 0.619647 8_4:-1724158 None:intergenic
TCGGAAATTGGCGGTGACGG+TGG 0.623894 8_4:-1724149 None:intergenic
GGTACGGTGGTTGTCCGCGG+TGG 0.629805 8_4:-1724441 None:intergenic
GTGGTGGTGCATCCAAATGG+TGG 0.658624 8_4:-1724316 None:intergenic
AATAGAGGATTGTTGGCTGG+AGG 0.659424 8_4:+1731699 Msa1306760:five_prime_UTR
CGGAAAGATGTAGATCTGGT+CGG 0.669353 8_4:-1724168 None:intergenic
GCGGTGACGGTGGTTCATGG+TGG 0.671713 8_4:-1724139 None:intergenic
AATAAATTACTCCGGTACGG+TGG 0.683152 8_4:-1724454 None:intergenic
GAAATGACCTCTGAAGCCGG+CGG 0.757401 8_4:-1724411 None:intergenic
TCATTTCCGGCCAACCACCG+CGG 0.758942 8_4:+1724427 Msa1306760:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAAAAATGATATTTC+CGG + chr8_4:1724293-1724312 Msa1306760:five_prime_UTR 10.0%
!! AAAAAATAAATAAATTACTC+CGG - chr8_4:1724465-1724484 None:intergenic 10.0%
!!! ACTTTTAAAATAAAAATCTT+TGG + chr8_4:1724825-1724844 Msa1306760:intron 10.0%
!!! ATAAAATTAGTTATTTGTTA+CGG - chr8_4:1725057-1725076 None:intergenic 10.0%
!! TAATAAAATCAAAAATTGTA+AGG + chr8_4:1725188-1725207 Msa1306760:intron 10.0%
!!! ATGTTTGATTTTAATTTATA+AGG - chr8_4:1725231-1725250 None:intergenic 10.0%
!! TAATAAAATAATTTCAAACA+TGG - chr8_4:1725547-1725566 None:intergenic 10.0%
!! AAAAATTATAAATAAACCAA+TGG + chr8_4:1727480-1727499 Msa1306760:intron 10.0%
!! AAATAAATAAAATGATTCTA+AGG + chr8_4:1728933-1728952 Msa1306760:intron 10.0%
!!! TTTTCAATTATTTTTTTCAA+GGG - chr8_4:1730756-1730775 None:intergenic 10.0%
!!! ATTTTCAATTATTTTTTTCA+AGG - chr8_4:1730757-1730776 None:intergenic 10.0%
!!! TAAGTTAATTTTTTTTTTAC+AGG + chr8_4:1732261-1732280 Msa1306760:intron 10.0%
!!! ATGTATTTTTTTTGTAGTAA+AGG - chr8_4:1724198-1724217 None:intergenic 15.0%
!!! AAATTTGTTTCAGTTTTATT+GGG - chr8_4:1724988-1725007 None:intergenic 15.0%
!!! AAAATTTGTTTCAGTTTTAT+TGG - chr8_4:1724989-1725008 None:intergenic 15.0%
!!! TAAAATTAGTTATTTGTTAC+GGG - chr8_4:1725056-1725075 None:intergenic 15.0%
!! AATTTAATATATGCTGAATA+TGG - chr8_4:1725128-1725147 None:intergenic 15.0%
!! AAAAAAGTAGATTGAATAAT+AGG + chr8_4:1725877-1725896 Msa1306760:intron 15.0%
!! GAATAAAAACAAGAAAATAA+AGG - chr8_4:1726167-1726186 None:intergenic 15.0%
!! ATATCTTATATGCATAAAAA+TGG - chr8_4:1726444-1726463 None:intergenic 15.0%
!!! AGATTATGTCAAATTTATTT+TGG - chr8_4:1726652-1726671 None:intergenic 15.0%
!!! AAGAAAGTAAGAAATAATTT+TGG + chr8_4:1729348-1729367 Msa1306760:intron 15.0%
!! AATATAAACTGAAAATAACT+TGG + chr8_4:1729849-1729868 Msa1306760:intron 15.0%
!! AAAATCAAATCATAATGATT+TGG - chr8_4:1730880-1730899 None:intergenic 15.0%
!!! ACATATTTATTTTTTTGTGA+GGG - chr8_4:1730997-1731016 None:intergenic 15.0%
!!! AACATATTTATTTTTTTGTG+AGG - chr8_4:1730998-1731017 None:intergenic 15.0%
!! AAATAAATATGTTATCACTT+TGG + chr8_4:1731005-1731024 Msa1306760:intron 15.0%
!!! TTTTTTTTCTATACTTTCTT+TGG + chr8_4:1731183-1731202 Msa1306760:intron 15.0%
!! AATAAAAAACATAATTTGGT+AGG + chr8_4:1731239-1731258 Msa1306760:intron 15.0%
!! AGAATTACTAAATTACTAAT+TGG - chr8_4:1732598-1732617 None:intergenic 15.0%
!!! TCTCAAATTAGATTTTCAAA+AGG + chr8_4:1724250-1724269 Msa1306760:five_prime_UTR 20.0%
!!! AAAAGGATGTTTTATAGAAT+GGG - chr8_4:1724357-1724376 None:intergenic 20.0%
!!! AAAAAGGATGTTTTATAGAA+TGG - chr8_4:1724358-1724377 None:intergenic 20.0%
!! ATAAATCGGAGAAATAAAAA+AGG - chr8_4:1724374-1724393 None:intergenic 20.0%
!! GTCTTAAAGATTAATTTCAA+TGG - chr8_4:1724859-1724878 None:intergenic 20.0%
!!! CTTTTGTTTTTGTTGTATTA+TGG - chr8_4:1724942-1724961 None:intergenic 20.0%
!!! ACTATAATGTTTGTTTTGTT+TGG - chr8_4:1725026-1725045 None:intergenic 20.0%
!!! TTAAATTCCTTATACCTTTT+TGG + chr8_4:1725141-1725160 Msa1306760:intron 20.0%
!!! TTTGAACTTCAATTTGATTT+TGG - chr8_4:1725260-1725279 None:intergenic 20.0%
!! ATCTTCACAAAAGATTAATT+AGG + chr8_4:1725980-1725999 Msa1306760:intron 20.0%
!! TCTTCACAAAAGATTAATTA+GGG + chr8_4:1725981-1726000 Msa1306760:intron 20.0%
!! TGTCACAAATTTATCAATTT+CGG - chr8_4:1726230-1726249 None:intergenic 20.0%
!! TTTGTGACATATTGATAAAT+TGG + chr8_4:1726241-1726260 Msa1306760:intron 20.0%
!! TTGTGACATATTGATAAATT+GGG + chr8_4:1726242-1726261 Msa1306760:intron 20.0%
!! GAAAAAATAAATTCCAAATC+TGG - chr8_4:1726268-1726287 None:intergenic 20.0%
!!! TTTTATTTGATTTTCATGAG+TGG + chr8_4:1726303-1726322 Msa1306760:intron 20.0%
!! TGAATGAATCAGTAAAAATT+GGG - chr8_4:1726507-1726526 None:intergenic 20.0%
!! AAAAACTGAATTTGAGTATA+AGG - chr8_4:1726554-1726573 None:intergenic 20.0%
!! AATTCAATAAACATACTCTA+AGG - chr8_4:1726719-1726738 None:intergenic 20.0%
!! AACATTTATTAGAGAATGTT+TGG - chr8_4:1726797-1726816 None:intergenic 20.0%
!!! TGTATAAAGTACTCAAAATA+AGG - chr8_4:1726826-1726845 None:intergenic 20.0%
!!! ACATATTGTACTTCATTTTT+TGG + chr8_4:1727089-1727108 Msa1306760:intron 20.0%
!! TATTACATTCGAATCATTAT+TGG - chr8_4:1728026-1728045 None:intergenic 20.0%
!!! GAAATTGATATGTATTTTGT+AGG + chr8_4:1728383-1728402 Msa1306760:intron 20.0%
!! TTTATGGAAAGCAAAATTTA+TGG + chr8_4:1728615-1728634 Msa1306760:intron 20.0%
!!! TTTTAAAAGTTGAGAAGTTA+GGG - chr8_4:1728916-1728935 None:intergenic 20.0%
!!! ATTTTAAAAGTTGAGAAGTT+AGG - chr8_4:1728917-1728936 None:intergenic 20.0%
!!! AGGATCTTGTATTAATTTTT+TGG + chr8_4:1729551-1729570 Msa1306760:intron 20.0%
!! ACGATTATATTAGATAGAAA+AGG - chr8_4:1729646-1729665 None:intergenic 20.0%
!! ATGATACAAGCAAAATATAT+TGG - chr8_4:1730144-1730163 None:intergenic 20.0%
!! TTGATGTATTTATAGAGAAT+AGG + chr8_4:1730497-1730516 Msa1306760:intron 20.0%
!!! TAAATTGAAATGTGATTTGT+AGG + chr8_4:1730554-1730573 Msa1306760:intron 20.0%
!!! AAGGATGTTTTTGTTTTATA+CGG + chr8_4:1730728-1730747 Msa1306760:intron 20.0%
!! CTTCTTTCAACAAAAAAAAA+GGG - chr8_4:1731301-1731320 None:intergenic 20.0%
!! GATTCATAGATAAACTTAAA+TGG + chr8_4:1731352-1731371 Msa1306760:intron 20.0%
!!! TTTTATTTCCTCATTCTTAA+TGG + chr8_4:1731400-1731419 Msa1306760:intron 20.0%
!!! ATAAAAGTGGTTTAAAATCA+AGG + chr8_4:1732382-1732401 Msa1306760:intron 20.0%
!! AAATAAAATTGAAGAGAAGA+TGG + chr8_4:1732898-1732917 Msa1306760:CDS 20.0%
!! TTTGAATATATGGTTTCAAA+AGG + chr8_4:1733147-1733166 Msa1306760:three_prime_UTR 20.0%
! GAAAAAGATATGAATGGAAA+CGG - chr8_4:1724115-1724134 None:intergenic 25.0%
!! AAAGGATGTTTTATAGAATG+GGG - chr8_4:1724356-1724375 None:intergenic 25.0%
! GAAAGATCAATAGGATAAAT+CGG - chr8_4:1724388-1724407 None:intergenic 25.0%
! ATAAATAAATTACTCCGGTA+CGG - chr8_4:1724460-1724479 None:intergenic 25.0%
!! TGGTAAATTCATTTTAGACA+TGG + chr8_4:1724510-1724529 Msa1306760:intron 25.0%
!!! TTCAAAATGGTTAATTTGCT+GGG - chr8_4:1724701-1724720 None:intergenic 25.0%
!!! TTTCAAAATGGTTAATTTGC+TGG - chr8_4:1724702-1724721 None:intergenic 25.0%
!!! AATTAACCATTTTGAAATGC+AGG + chr8_4:1724705-1724724 Msa1306760:intron 25.0%
! TTAAATCCTGCATTTCAAAA+TGG - chr8_4:1724714-1724733 None:intergenic 25.0%
!!! GGATAATTGTTTTGAAGTTA+AGG - chr8_4:1724743-1724762 None:intergenic 25.0%
!! GAAAACTAATTGATAATCCA+TGG + chr8_4:1724796-1724815 Msa1306760:intron 25.0%
!!! ATTTTAAAAGTCGTAAACCA+TGG - chr8_4:1724816-1724835 None:intergenic 25.0%
!!! AATGTTTGTTTTGTTTGGTA+AGG - chr8_4:1725021-1725040 None:intergenic 25.0%
! ATGTAATTGAAGAGTGATTT+AGG - chr8_4:1725169-1725188 None:intergenic 25.0%
! TTTAAATAGAGAGATAGACA+AGG - chr8_4:1725847-1725866 None:intergenic 25.0%
! TATGAGATTGAAATTGAGAA+GGG - chr8_4:1726127-1726146 None:intergenic 25.0%
! ATATGAGATTGAAATTGAGA+AGG - chr8_4:1726128-1726147 None:intergenic 25.0%
! AATGCATTGTAAGCATAAAA+GGG - chr8_4:1726345-1726364 None:intergenic 25.0%
! TAATGCATTGTAAGCATAAA+AGG - chr8_4:1726346-1726365 None:intergenic 25.0%
! ATGCATATAAGATATTACCA+TGG + chr8_4:1726449-1726468 Msa1306760:intron 25.0%
! GTGAATGAATCAGTAAAAAT+TGG - chr8_4:1726508-1726527 None:intergenic 25.0%
!! GAATGTTTGGAATATATTAG+AGG - chr8_4:1726784-1726803 None:intergenic 25.0%
! TTTAATTAGTCACTGAACTA+TGG + chr8_4:1726871-1726890 Msa1306760:intron 25.0%
! ATCAGCAAAATTTGAGTATT+GGG - chr8_4:1726924-1726943 None:intergenic 25.0%
! TCTTAGAGTAATAGAATAGA+TGG + chr8_4:1727027-1727046 Msa1306760:intron 25.0%
! CTTAGAGTAATAGAATAGAT+GGG + chr8_4:1727028-1727047 Msa1306760:intron 25.0%
! GGAAGAAATTCAAAAAGATT+AGG + chr8_4:1727110-1727129 Msa1306760:intron 25.0%
! AAAAATATCGTTCCTCAATA+TGG - chr8_4:1727402-1727421 None:intergenic 25.0%
! ATATAACTCCTTCTAATAGT+TGG + chr8_4:1727960-1727979 Msa1306760:intron 25.0%
! AATTCAAACCAACTATTAGA+AGG - chr8_4:1727971-1727990 None:intergenic 25.0%
! TTTGTTTAAAGAATGCATAG+TGG + chr8_4:1727989-1728008 Msa1306760:intron 25.0%
!! AATTAGCAGAGTTTAAAATG+TGG - chr8_4:1728163-1728182 None:intergenic 25.0%
! TTAACATGCATTATTATCTG+AGG + chr8_4:1728197-1728216 Msa1306760:intron 25.0%
! TAACATGCATTATTATCTGA+GGG + chr8_4:1728198-1728217 Msa1306760:intron 25.0%
!!! CTACTTTTGTGATTAGTTTA+AGG - chr8_4:1728299-1728318 None:intergenic 25.0%
! ATTTCATTGTTGCTCTATAA+GGG - chr8_4:1728368-1728387 None:intergenic 25.0%
! AATTTCATTGTTGCTCTATA+AGG - chr8_4:1728369-1728388 None:intergenic 25.0%
!! TATGTATTTTGTAGGTTAGT+AGG + chr8_4:1728391-1728410 Msa1306760:intron 25.0%
!!! AGTAGGATTTTTGACTTTTT+TGG + chr8_4:1728408-1728427 Msa1306760:intron 25.0%
! AGTACGGTAAAGTTAAAAAA+TGG - chr8_4:1728537-1728556 None:intergenic 25.0%
!! TTCCATAAATTTTCATTTGG+TGG - chr8_4:1728604-1728623 None:intergenic 25.0%
!! GCTTTCCATAAATTTTCATT+TGG - chr8_4:1728607-1728626 None:intergenic 25.0%
! CAGATTCACAAATTTCATTT+GGG - chr8_4:1728743-1728762 None:intergenic 25.0%
! TCAGATTCACAAATTTCATT+TGG - chr8_4:1728744-1728763 None:intergenic 25.0%
!!! TGATTCTAAGGTAATAGAAA+TGG + chr8_4:1728945-1728964 Msa1306760:intron 25.0%
!!! GATTCTAAGGTAATAGAAAT+GGG + chr8_4:1728946-1728965 Msa1306760:intron 25.0%
! CAATAGAATACATCAATTAC+AGG - chr8_4:1729003-1729022 None:intergenic 25.0%
! CTGTAATTGATGTATTCTAT+TGG + chr8_4:1729001-1729020 Msa1306760:intron 25.0%
! ATAGCATGATAAGAAGAAAA+AGG + chr8_4:1729152-1729171 Msa1306760:intron 25.0%
!! TTTTCAAAGAGAATAGTGTT+GGG - chr8_4:1729504-1729523 None:intergenic 25.0%
!! ATTTTCAAAGAGAATAGTGT+TGG - chr8_4:1729505-1729524 None:intergenic 25.0%
! AACAGATAAATTTAGTGACA+TGG - chr8_4:1729953-1729972 None:intergenic 25.0%
! TGTAATGAAAATGCCTAAAA+GGG - chr8_4:1730013-1730032 None:intergenic 25.0%
! TTGTAATGAAAATGCCTAAA+AGG - chr8_4:1730014-1730033 None:intergenic 25.0%
! AATCTTATTAACATCTCTGA+CGG + chr8_4:1730032-1730051 Msa1306760:intron 25.0%
!!! ACAATTTATTTTCACTACAC+AGG - chr8_4:1730064-1730083 None:intergenic 25.0%
!! ATTTTGCTTGTATCATAAGA+AGG + chr8_4:1730148-1730167 Msa1306760:intron 25.0%
!! TTTTGCTTGTATCATAAGAA+GGG + chr8_4:1730149-1730168 Msa1306760:intron 25.0%
!!! TTTTTTAGAGAATTTCAGCA+TGG + chr8_4:1730291-1730310 Msa1306760:intron 25.0%
! TGTATTTATAGAGAATAGGA+TGG + chr8_4:1730501-1730520 Msa1306760:intron 25.0%
! AAAATGTGAGAAAAACAGTT+GGG + chr8_4:1730799-1730818 Msa1306760:intron 25.0%
! TGCATTATGTGTATTCATAT+TGG + chr8_4:1731107-1731126 Msa1306760:intron 25.0%
! GCTTCTTTCAACAAAAAAAA+AGG - chr8_4:1731302-1731321 None:intergenic 25.0%
! ACAAATAGTCTTTCTTGAAA+GGG - chr8_4:1731434-1731453 None:intergenic 25.0%
! AACAAATAGTCTTTCTTGAA+AGG - chr8_4:1731435-1731454 None:intergenic 25.0%
!!! ATTTTTATTTGGCAGAATAG+AGG + chr8_4:1731684-1731703 Msa1306760:intron 25.0%
!! TTTTATGTTCTTCATTTTGC+AGG - chr8_4:1731747-1731766 None:intergenic 25.0%
! GCAAAATGAAGAACATAAAA+TGG + chr8_4:1731747-1731766 Msa1306760:five_prime_UTR 25.0%
!! TTTTCCTCTTGAAATGTTTT+TGG + chr8_4:1731834-1731853 Msa1306760:intron 25.0%
! ATTAACTTATCAGATCTACA+AGG - chr8_4:1732250-1732269 None:intergenic 25.0%
!!! TTAAACCACTTTTATTTGCT+AGG - chr8_4:1732377-1732396 None:intergenic 25.0%
!! TTTCAGTATTATTTTCTTGC+AGG - chr8_4:1732540-1732559 None:intergenic 25.0%
!!! TCTCTTCAATTTTATTTTGC+AGG - chr8_4:1732895-1732914 None:intergenic 25.0%
!! ATGTGATTTTTAACAAGTGA+GGG - chr8_4:1733036-1733055 None:intergenic 25.0%
! AAGTGAGAATGAAAAATATG+AGG + chr8_4:1733116-1733135 Msa1306760:three_prime_UTR 25.0%
!! GGAAAGTGATTTTGAATATA+TGG + chr8_4:1733137-1733156 Msa1306760:three_prime_UTR 25.0%
! AATATATGGTTTCAAAAGGT+TGG + chr8_4:1733151-1733170 Msa1306760:three_prime_UTR 25.0%
GGAGAAGAAAAAGATATGAA+TGG - chr8_4:1724121-1724140 None:intergenic 30.0%
CTAATTTGAGAGAAAGATAG+AGG - chr8_4:1724241-1724260 None:intergenic 30.0%
AATGAATTTACCAAGAAGAG+AGG - chr8_4:1724503-1724522 None:intergenic 30.0%
TATGGCTTATTCGAAAATCA+TGG - chr8_4:1724677-1724696 None:intergenic 30.0%
CAAAACAATTATCCGATCAT+TGG + chr8_4:1724749-1724768 Msa1306760:intron 30.0%
CAAACTGAACTAAATAGTAG+AGG + chr8_4:1724878-1724897 Msa1306760:intron 30.0%
! TTTACCTCCACATTTTACTA+TGG - chr8_4:1724909-1724928 None:intergenic 30.0%
!! TATTGGGTATTTTTGCTCAT+GGG - chr8_4:1724972-1724991 None:intergenic 30.0%
!! TTATTGGGTATTTTTGCTCA+TGG - chr8_4:1724973-1724992 None:intergenic 30.0%
! TTAGGTACCAAAAAGGTATA+AGG - chr8_4:1725151-1725170 None:intergenic 30.0%
TCAGAATAGGAGAAATGATT+GGG - chr8_4:1725697-1725716 None:intergenic 30.0%
TTCAGAATAGGAGAAATGAT+TGG - chr8_4:1725698-1725717 None:intergenic 30.0%
ATGAGATTGAAATTGAGAAG+GGG - chr8_4:1726126-1726145 None:intergenic 30.0%
ATGCATTGTAAGCATAAAAG+GGG - chr8_4:1726344-1726363 None:intergenic 30.0%
GTCACAAAATGACATTTGAA+AGG - chr8_4:1726589-1726608 None:intergenic 30.0%
!!! TTCAAATGTCATTTTGTGAC+TGG + chr8_4:1726589-1726608 Msa1306760:intron 30.0%
CATCAGCAAAATTTGAGTAT+TGG - chr8_4:1726925-1726944 None:intergenic 30.0%
! CAATACTCAAATTTTGCTGA+TGG + chr8_4:1726923-1726942 Msa1306760:intron 30.0%
! TTCTCTGTTAGTTCTTCTTT+AGG - chr8_4:1727070-1727089 None:intergenic 30.0%
! AGTCAGATCAAATATTGACT+TGG - chr8_4:1727203-1727222 None:intergenic 30.0%
TCGATTATTAGTACTTTCCT+TGG + chr8_4:1727314-1727333 Msa1306760:intron 30.0%
! AATTTAGAATTGAAGCTCCA+AGG - chr8_4:1727334-1727353 None:intergenic 30.0%
TGAAATCATGTTCCATATTG+AGG + chr8_4:1727374-1727393 Msa1306760:intron 30.0%
CATATAAATAGCTCTTGAGA+TGG + chr8_4:1727598-1727617 Msa1306760:intron 30.0%
GACATAATAAGACATGATGA+GGG - chr8_4:1727652-1727671 None:intergenic 30.0%
! TCTCTACCTAGATTTGAAAT+GGG + chr8_4:1727711-1727730 Msa1306760:intron 30.0%
TAGTAACCCATTTCAAATCT+AGG - chr8_4:1727720-1727739 None:intergenic 30.0%
GTGATAAGTGTAGAAATGAT+GGG + chr8_4:1727873-1727892 Msa1306760:intron 30.0%
AAAGATGTGTTAGATAGGAA+AGG - chr8_4:1727921-1727940 None:intergenic 30.0%
CAGAAAAAGATGTGTTAGAT+AGG - chr8_4:1727926-1727945 None:intergenic 30.0%
ATTCGAATCATTATTGGAAG+TGG - chr8_4:1728020-1728039 None:intergenic 30.0%
GAAGAAATGAGTGTATACTA+AGG - chr8_4:1728250-1728269 None:intergenic 30.0%
! ATTTTGTACTCCACAATTCA+AGG + chr8_4:1728435-1728454 Msa1306760:intron 30.0%
!! GGGGATTTTAAAAGTACATT+CGG - chr8_4:1728493-1728512 None:intergenic 30.0%
!!! TTTTAAAATCCCCCCAAATT+TGG + chr8_4:1728500-1728519 Msa1306760:intron 30.0%
CGGTAAAGTTAAAAAATGGT+TGG - chr8_4:1728533-1728552 None:intergenic 30.0%
CCATAAATTTCATGCAAGTA+CGG - chr8_4:1728553-1728572 None:intergenic 30.0%
!! CATAAATTTTCATTTGGTGG+TGG - chr8_4:1728601-1728620 None:intergenic 30.0%
CACCACCAAATGAAAATTTA+TGG + chr8_4:1728599-1728618 Msa1306760:intron 30.0%
GTACGGTAAGAAATTTCATT+TGG - chr8_4:1728677-1728696 None:intergenic 30.0%
TCACTGAATGAAATAGAATG+TGG + chr8_4:1728811-1728830 Msa1306760:intron 30.0%
!! AGAATGTGGAAATTTTGAGT+AGG + chr8_4:1728825-1728844 Msa1306760:intron 30.0%
!!! AATGCTTTTTTCTCAACTCT+TGG + chr8_4:1728885-1728904 Msa1306760:intron 30.0%
AACATAAAATTTGGCCAATG+GGG - chr8_4:1729056-1729075 None:intergenic 30.0%
CAACATAAAATTTGGCCAAT+GGG - chr8_4:1729057-1729076 None:intergenic 30.0%
! CATTGGCCAAATTTTATGTT+GGG + chr8_4:1729056-1729075 Msa1306760:intron 30.0%
GGAAAACCCAACATAAAATT+TGG - chr8_4:1729065-1729084 None:intergenic 30.0%
GCAATGAAATTCAAGGTAAA+TGG - chr8_4:1729101-1729120 None:intergenic 30.0%
! TTTTCAGTTATGCACAAGAT+TGG + chr8_4:1729291-1729310 Msa1306760:intron 30.0%
TATTTGGCTAAATTTCTGTC+TGG + chr8_4:1729440-1729459 Msa1306760:intron 30.0%
ATCTTGCTTGTTTCATAAGA+AGG + chr8_4:1729874-1729893 Msa1306760:intron 30.0%
TCTTGCTTGTTTCATAAGAA+GGG + chr8_4:1729875-1729894 Msa1306760:intron 30.0%
TTTGCTTGTATCATAAGAAG+GGG + chr8_4:1730150-1730169 Msa1306760:intron 30.0%
! CATTTCTTTGTTGATGAAGA+TGG + chr8_4:1730226-1730245 Msa1306760:intron 30.0%
TTTCAGCAAGGATTAATTGT+GGG + chr8_4:1730258-1730277 Msa1306760:intron 30.0%
!! ACATCAAGCATTTTGATCAT+TGG - chr8_4:1730484-1730503 None:intergenic 30.0%
AAGAAAATCATTTGCTCACA+TGG - chr8_4:1730653-1730672 None:intergenic 30.0%
TGCAAAGAAAACATTTGCAT+GGG - chr8_4:1730681-1730700 None:intergenic 30.0%
TTGCAAAGAAAACATTTGCA+TGG - chr8_4:1730682-1730701 None:intergenic 30.0%
CAAAATGTGAGAAAAACAGT+TGG + chr8_4:1730798-1730817 Msa1306760:intron 30.0%
AGAAAAACAGTTGGGATTTA+GGG + chr8_4:1730807-1730826 Msa1306760:intron 30.0%
! CTTGATTTCTAGATTTGAAC+TGG - chr8_4:1730925-1730944 None:intergenic 30.0%
TCAACAAAAAAAAAGGGTCA+AGG - chr8_4:1731295-1731314 None:intergenic 30.0%
AAACATGTCCATTAAGAATG+AGG - chr8_4:1731411-1731430 None:intergenic 30.0%
GAAAAGAATATGACACTTTC+AGG - chr8_4:1731511-1731530 None:intergenic 30.0%
AGATCCAAAAACATTTCAAG+AGG - chr8_4:1731841-1731860 None:intergenic 30.0%
CAGCAGAAAGTTATACTTAA+CGG + chr8_4:1731905-1731924 Msa1306760:intron 30.0%
AGCAGAAAGTTATACTTAAC+GGG + chr8_4:1731906-1731925 Msa1306760:intron 30.0%
GATCAGAAAAAACAAAGAAG+AGG + chr8_4:1732104-1732123 Msa1306760:five_prime_UTR 30.0%
GATCAGAAAAAACAAAGAAG+AGG + chr8_4:1732104-1732123 Msa1306760:five_prime_UTR 30.0%
GAAAACCTAGCAAATAAAAG+TGG + chr8_4:1732369-1732388 Msa1306760:CDS 30.0%
TGATCTAGAAATGTTATTGC+AGG - chr8_4:1732692-1732711 None:intergenic 30.0%
! ACTCTTCACTTCTATTTTGT+AGG - chr8_4:1732943-1732962 None:intergenic 30.0%
TACAAAATAGAAGTGAAGAG+TGG + chr8_4:1732942-1732961 Msa1306760:CDS 30.0%
! GATGTGATTTTTAACAAGTG+AGG - chr8_4:1733037-1733056 None:intergenic 30.0%
AAGATATGAATGGAAACGGA+TGG - chr8_4:1724111-1724130 None:intergenic 35.0%
!!! TTTTTTGTAGTAAAGGTCGC+CGG - chr8_4:1724191-1724210 None:intergenic 35.0%
!! ATGGTTAATTTGCTGGGTTA+TGG - chr8_4:1724695-1724714 None:intergenic 35.0%
TTAACCAACGTACCAATGAT+CGG - chr8_4:1724764-1724783 None:intergenic 35.0%
!! ATTGGGTATTTTTGCTCATG+GGG - chr8_4:1724971-1724990 None:intergenic 35.0%
GACTTCTTCGTACATGAAAT+TGG + chr8_4:1725082-1725101 Msa1306760:intron 35.0%
GAGTGATTTAGGTACCAAAA+AGG - chr8_4:1725158-1725177 None:intergenic 35.0%
!! GCAATTTGATTTGGGAGATT+GGG - chr8_4:1725660-1725679 None:intergenic 35.0%
!! TGCAATTTGATTTGGGAGAT+TGG - chr8_4:1725661-1725680 None:intergenic 35.0%
! TAGAGGCTGCAATTTGATTT+GGG - chr8_4:1725668-1725687 None:intergenic 35.0%
ATAGAGGCTGCAATTTGATT+TGG - chr8_4:1725669-1725688 None:intergenic 35.0%
AACTGTTAGGGTTTCAGAAT+AGG - chr8_4:1725710-1725729 None:intergenic 35.0%
! AGATTCTGCGTTTTCTGTTT+GGG - chr8_4:1725964-1725983 None:intergenic 35.0%
! AAGATTCTGCGTTTTCTGTT+TGG - chr8_4:1725965-1725984 None:intergenic 35.0%
TTAATTAGGGTTACGAATCG+TGG + chr8_4:1725994-1726013 Msa1306760:intron 35.0%
TTATTGACCTGAGAGATGAA+AGG - chr8_4:1726103-1726122 None:intergenic 35.0%
TCACTGTTAGATGAACTCAT+AGG + chr8_4:1726194-1726213 Msa1306760:intron 35.0%
TTGATAAATTGGGCCAGATT+TGG + chr8_4:1726252-1726271 Msa1306760:intron 35.0%
TGACATTTGAAAGGAAGTCA+AGG - chr8_4:1726580-1726599 None:intergenic 35.0%
! TTACAGAAGCATGTGAACAA+AGG + chr8_4:1726679-1726698 Msa1306760:intron 35.0%
CTCAATATGGTTCCTCAATA+TGG - chr8_4:1727389-1727408 None:intergenic 35.0%
CATATTGAGGAACCATATTG+AGG + chr8_4:1727387-1727406 Msa1306760:intron 35.0%
! AGCTTTTGAACAAAAGCCAT+TGG - chr8_4:1727499-1727518 None:intergenic 35.0%
TAAGACATGATGAGGGTTAT+GGG - chr8_4:1727645-1727664 None:intergenic 35.0%
ATAAGACATGATGAGGGTTA+TGG - chr8_4:1727646-1727665 None:intergenic 35.0%
CGACATAATAAGACATGATG+AGG - chr8_4:1727653-1727672 None:intergenic 35.0%
CTCTCTACCTAGATTTGAAA+TGG + chr8_4:1727710-1727729 Msa1306760:intron 35.0%
TGAACACTCATGAAATGAGA+TGG + chr8_4:1727762-1727781 Msa1306760:intron 35.0%
ACTCATGAAATGAGATGGAA+GGG + chr8_4:1727767-1727786 Msa1306760:intron 35.0%
AAGGTTCTTCCTATTCTTCA+AGG + chr8_4:1727792-1727811 Msa1306760:intron 35.0%
AAAGCTACTCCTTGAAGAAT+AGG - chr8_4:1727804-1727823 None:intergenic 35.0%
GAAGAAGCAATAGTTATGCA+AGG + chr8_4:1727847-1727866 Msa1306760:intron 35.0%
AAGCAATAGTTATGCAAGGA+AGG + chr8_4:1727851-1727870 Msa1306760:intron 35.0%
GGTGATAAGTGTAGAAATGA+TGG + chr8_4:1727872-1727891 Msa1306760:intron 35.0%
TTTATTCCTCTGTCTGAATC+TGG + chr8_4:1728117-1728136 Msa1306760:intron 35.0%
TATTATCCAGATTCAGACAG+AGG - chr8_4:1728126-1728145 None:intergenic 35.0%
ATGGTAAAAGCCTTGAATTG+TGG - chr8_4:1728448-1728467 None:intergenic 35.0%
! TCAAGGCTTTTACCATGTAT+TGG + chr8_4:1728452-1728471 Msa1306760:intron 35.0%
AAAAATGCAGCTCCAATACA+TGG - chr8_4:1728467-1728486 None:intergenic 35.0%
TTGGACAAGATCCAAATTTG+GGG - chr8_4:1728514-1728533 None:intergenic 35.0%
GTTGGACAAGATCCAAATTT+GGG - chr8_4:1728515-1728534 None:intergenic 35.0%
CCGTACTTGCATGAAATTTA+TGG + chr8_4:1728550-1728569 Msa1306760:intron 35.0%
! TGTAGAAAAGAGTGCAAGTA+CGG - chr8_4:1728694-1728713 None:intergenic 35.0%
AAAGTTGAGAAGTTAGGGTT+TGG - chr8_4:1728911-1728930 None:intergenic 35.0%
CCAACATAAAATTTGGCCAA+TGG - chr8_4:1729058-1729077 None:intergenic 35.0%
! CCATTGGCCAAATTTTATGT+TGG + chr8_4:1729055-1729074 Msa1306760:intron 35.0%
TTTCTGTCTGGATTCATTGT+AGG + chr8_4:1729452-1729471 Msa1306760:intron 35.0%
! TATTTAACTTCTCACTGCAC+AGG - chr8_4:1729738-1729757 None:intergenic 35.0%
TGAACGTATTATACTTGTGC+AGG + chr8_4:1729773-1729792 Msa1306760:intron 35.0%
CTTGCTTGTTTCATAAGAAG+GGG + chr8_4:1729876-1729895 Msa1306760:intron 35.0%
AAGGGGAAAGACAGAAAAAA+AGG + chr8_4:1729893-1729912 Msa1306760:intron 35.0%
AGGGGAAAGACAGAAAAAAA+GGG + chr8_4:1729894-1729913 Msa1306760:intron 35.0%
CATTAGACATCATTGCTAGT+TGG + chr8_4:1730092-1730111 Msa1306760:intron 35.0%
GTATCATAAGAAGGGGAAAA+CGG + chr8_4:1730157-1730176 Msa1306760:intron 35.0%
! TTCTTTGTTGATGAAGATGG+TGG + chr8_4:1730229-1730248 Msa1306760:intron 35.0%
! TCTTTGTTGATGAAGATGGT+GGG + chr8_4:1730230-1730249 Msa1306760:intron 35.0%
! TTTCAGCATGGATTAAGTGT+AGG + chr8_4:1730303-1730322 Msa1306760:intron 35.0%
!! CAATAGCTTGTTGATGAAGA+TGG + chr8_4:1730334-1730353 Msa1306760:intron 35.0%
GTTTCAGCAAGGATTAATTG+TGG + chr8_4:1730257-1730276 Msa1306760:intron 35.0%
AGAAGGAAGTGAAGAAAGAA+AGG + chr8_4:1730429-1730448 Msa1306760:intron 35.0%
! AATGTGATTTGTAGGCACAT+AGG + chr8_4:1730562-1730581 Msa1306760:intron 35.0%
TGCAAATGTTTGCTTGGATA+AGG + chr8_4:1730709-1730728 Msa1306760:intron 35.0%
GAGAAAAACAGTTGGGATTT+AGG + chr8_4:1730806-1730825 Msa1306760:intron 35.0%
TCTAGATTTGAACTGGACAA+AGG - chr8_4:1730918-1730937 None:intergenic 35.0%
CGTTAAGTATAACTTTCTGC+TGG - chr8_4:1731907-1731926 None:intergenic 35.0%
CTTCAGCAAACTACAAAGAA+AGG - chr8_4:1731997-1732016 None:intergenic 35.0%
TTTGCAACTGAAAGTTGAAG+TGG - chr8_4:1732288-1732307 None:intergenic 35.0%
CTTCAACTTTCAGTTGCAAA+AGG + chr8_4:1732288-1732307 Msa1306760:CDS 35.0%
GTTATTGCAGGAAAGTTGAA+TGG - chr8_4:1732680-1732699 None:intergenic 35.0%
AGAAATGATGAGGAAGATCA+AGG - chr8_4:1733010-1733029 None:intergenic 35.0%
ATCTTGTGAAGATTTCTGCT+TGG + chr8_4:1733085-1733104 Msa1306760:three_prime_UTR 35.0%
GAGAGATTGTGGAAACTGAT+AGG - chr8_4:1724056-1724075 None:intergenic 40.0%
GGGAAATATGTGAGAGATTG+TGG - chr8_4:1724067-1724086 None:intergenic 40.0%
ATGTAGATCTGGTCGGAAAT+TGG - chr8_4:1724164-1724183 None:intergenic 40.0%
GAGAAAGATAGAGGAGAATG+AGG - chr8_4:1724232-1724251 None:intergenic 40.0%
! GGATGTTTTATAGAATGGGG+AGG - chr8_4:1724353-1724372 None:intergenic 40.0%
AATAAATTACTCCGGTACGG+TGG - chr8_4:1724457-1724476 None:intergenic 40.0%
AGATCATCAACCTCTCTTCT+TGG + chr8_4:1724490-1724509 Msa1306760:five_prime_UTR 40.0%
CGCACGGGTAAATTTATCTT+CGG + chr8_4:1724577-1724596 Msa1306760:intron 40.0%
TTATCCGATCATTGGTACGT+TGG + chr8_4:1724757-1724776 Msa1306760:intron 40.0%
GGTTCCACCATAGTAAAATG+TGG + chr8_4:1724899-1724918 Msa1306760:intron 40.0%
TCCACCATAGTAAAATGTGG+AGG + chr8_4:1724902-1724921 Msa1306760:intron 40.0%
! ACCTCCACATTTTACTATGG+TGG - chr8_4:1724906-1724925 None:intergenic 40.0%
!! TTGGGTATTTTTGCTCATGG+GGG - chr8_4:1724970-1724989 None:intergenic 40.0%
GTACATGAAATTGGCGATTG+AGG + chr8_4:1725091-1725110 Msa1306760:intron 40.0%
TTAGATTGAGTGCCTTCATC+TGG + chr8_4:1725364-1725383 Msa1306760:intron 40.0%
! GTTATTCACACCACCGTTTT+AGG + chr8_4:1725598-1725617 Msa1306760:intron 40.0%
TCCCTCTTACAATCCAGAAT+TGG + chr8_4:1725628-1725647 Msa1306760:intron 40.0%
CCCAATTCTGGATTGTAAGA+GGG - chr8_4:1725632-1725651 None:intergenic 40.0%
CCCTCTTACAATCCAGAATT+GGG + chr8_4:1725629-1725648 Msa1306760:intron 40.0%
ACCCAATTCTGGATTGTAAG+AGG - chr8_4:1725633-1725652 None:intergenic 40.0%
AAATGATTGGGAGTGGATAG+AGG - chr8_4:1725685-1725704 None:intergenic 40.0%
ATAGGAGAAATGATTGGGAG+TGG - chr8_4:1725692-1725711 None:intergenic 40.0%
AAGATGAAGCGCAACTGTTA+GGG - chr8_4:1725722-1725741 None:intergenic 40.0%
AAAGATGAAGCGCAACTGTT+AGG - chr8_4:1725723-1725742 None:intergenic 40.0%
TATACGGCCGAGTTAAATCA+GGG + chr8_4:1726026-1726045 Msa1306760:intron 40.0%
CAGAGAACCCTGATTTAACT+CGG - chr8_4:1726036-1726055 None:intergenic 40.0%
CTCTGCTTCTTTCTTCTCAA+AGG + chr8_4:1726051-1726070 Msa1306760:intron 40.0%
TTCGTTTACGTTGGAAGGAA+AGG + chr8_4:1726475-1726494 Msa1306760:intron 40.0%
!! TTTGAGTATTGGGTGTGTGT+AGG - chr8_4:1726914-1726933 None:intergenic 40.0%
TGTCCAAGAGAGAAGTAACA+CGG + chr8_4:1726982-1727001 Msa1306760:intron 40.0%
AGAATAGATGGGCATGCATT+TGG + chr8_4:1727039-1727058 Msa1306760:intron 40.0%
GAATAGATGGGCATGCATTT+GGG + chr8_4:1727040-1727059 Msa1306760:intron 40.0%
CATTTGTCAATGCTAGCTGT+TGG + chr8_4:1727425-1727444 Msa1306760:intron 40.0%
!!! AATGCTAGCTGTTGGCTTTT+GGG + chr8_4:1727433-1727452 Msa1306760:intron 40.0%
AAGACATGATGAGGGTTATG+GGG - chr8_4:1727644-1727663 None:intergenic 40.0%
AATCTAGGTAGAGAGTTGCA+TGG - chr8_4:1727705-1727724 None:intergenic 40.0%
CACTCATGAAATGAGATGGA+AGG + chr8_4:1727766-1727785 Msa1306760:intron 40.0%
GAAATGAGATGGAAGGGAAA+AGG + chr8_4:1727773-1727792 Msa1306760:intron 40.0%
GCTTTCAAGAGTCTGTTGAA+AGG + chr8_4:1727819-1727838 Msa1306760:intron 40.0%
! CTGCAACATTTTCCATGCTA+TGG + chr8_4:1728070-1728089 Msa1306760:intron 40.0%
ACATGTGAGAAACCATAGCA+TGG - chr8_4:1728085-1728104 None:intergenic 40.0%
TGGACAAGATCCAAATTTGG+GGG - chr8_4:1728513-1728532 None:intergenic 40.0%
GGTTGGACAAGATCCAAATT+TGG - chr8_4:1728516-1728535 None:intergenic 40.0%
! TATGTTGGGTTTTCCTGTCT+TGG + chr8_4:1729070-1729089 Msa1306760:intron 40.0%
GTGCAGTGCAATGAAATTCA+AGG - chr8_4:1729108-1729127 None:intergenic 40.0%
TATTATACCTGTGCAGTGAG+AGG + chr8_4:1729677-1729696 Msa1306760:intron 40.0%
GGGGAAAGACAGAAAAAAAG+GGG + chr8_4:1729895-1729914 Msa1306760:intron 40.0%
GGGAAAGACAGAAAAAAAGG+GGG + chr8_4:1729896-1729915 Msa1306760:intron 40.0%
TAAAAGGGATCTTGATGAGG+AGG - chr8_4:1729998-1730017 None:intergenic 40.0%
! TCCTCATCAAGATCCCTTTT+AGG + chr8_4:1729997-1730016 Msa1306760:intron 40.0%
! CTTTGTTGATGAAGATGGTG+GGG + chr8_4:1730231-1730250 Msa1306760:intron 40.0%
!! TAGCTTGTTGATGAAGATGG+TGG + chr8_4:1730337-1730356 Msa1306760:intron 40.0%
!! AGCTTGTTGATGAAGATGGT+GGG + chr8_4:1730338-1730357 Msa1306760:intron 40.0%
!! CAGAAGCTTGTTGATGAAGA+TGG + chr8_4:1730397-1730416 Msa1306760:intron 40.0%
!! AAGCTTGTTGATGAAGATGG+TGG + chr8_4:1730400-1730419 Msa1306760:intron 40.0%
!! AGCTTGTTGATGAAGATGGT+GGG + chr8_4:1730338-1730357 Msa1306760:intron 40.0%
GGAAGTGAAGAAAGAAAGGA+AGG + chr8_4:1730433-1730452 Msa1306760:intron 40.0%
GTGAAGAAAGAAAGGAAGGA+AGG + chr8_4:1730437-1730456 Msa1306760:intron 40.0%
AGAAAGAAAGGAAGGAAGGA+AGG + chr8_4:1730441-1730460 Msa1306760:intron 40.0%
!!! TCTGTCTCATCCCTTGTTTT+TGG + chr8_4:1730619-1730638 Msa1306760:intron 40.0%
!!! CTGTCTCATCCCTTGTTTTT+GGG + chr8_4:1730620-1730639 Msa1306760:intron 40.0%
GGCAAACATCCCAAAAACAA+GGG - chr8_4:1730632-1730651 None:intergenic 40.0%
TGGCAAACATCCCAAAAACA+AGG - chr8_4:1730633-1730652 None:intergenic 40.0%
CAAGCAAACATTTGCATGTG+GGG - chr8_4:1730705-1730724 None:intergenic 40.0%
CCAAGCAAACATTTGCATGT+GGG - chr8_4:1730706-1730725 None:intergenic 40.0%
TCCAAGCAAACATTTGCATG+TGG - chr8_4:1730707-1730726 None:intergenic 40.0%
TGTTGAAAGAAGCCTTCTAC+TGG + chr8_4:1731309-1731328 Msa1306760:intron 40.0%
GTTGAAAGAAGCCTTCTACT+GGG + chr8_4:1731310-1731329 Msa1306760:intron 40.0%
TATGACACTTTCAGGACTCA+TGG - chr8_4:1731503-1731522 None:intergenic 40.0%
TTGGCAGAATAGAGGATTGT+TGG + chr8_4:1731692-1731711 Msa1306760:intron 40.0%
ACTACAAAGAAAGGAGACAC+TGG - chr8_4:1731988-1732007 None:intergenic 40.0%
TCTTGCAGGAAACGTTGAAT+TGG - chr8_4:1732526-1732545 None:intergenic 40.0%
CAGCACTTCAACTACTTGTT+GGG + chr8_4:1732846-1732865 Msa1306760:intron 40.0%
TTTCAGGCGAGAGAGAGAAA+GGG - chr8_4:1724088-1724107 None:intergenic 45.0%
GAATGGAAACGGATGGTTTC+AGG - chr8_4:1724104-1724123 None:intergenic 45.0%
TAGATCTGGTCGGAAATTGG+CGG - chr8_4:1724161-1724180 None:intergenic 45.0%
CGGAAAGATGTAGATCTGGT+CGG - chr8_4:1724171-1724190 None:intergenic 45.0%
CGACCAGATCTACATCTTTC+CGG + chr8_4:1724169-1724188 Msa1306760:five_prime_UTR 45.0%
TGATATTTCCGGCCACCATT+TGG + chr8_4:1724304-1724323 Msa1306760:five_prime_UTR 45.0%
!! GAATGGGGAGGTTAGTTTAG+TGG - chr8_4:1724341-1724360 None:intergenic 45.0%
CTATTGATCTTTCAGACCGC+CGG + chr8_4:1724395-1724414 Msa1306760:five_prime_UTR 45.0%
ACACGCGTTGTCAATCATTG+TGG - chr8_4:1724634-1724653 None:intergenic 45.0%
!! TGGGTATTTTTGCTCATGGG+GGG - chr8_4:1724969-1724988 None:intergenic 45.0%
CCTTCATCTGGATTCGAACT+CGG + chr8_4:1725376-1725395 Msa1306760:intron 45.0%
CTTCATCTGGATTCGAACTC+GGG + chr8_4:1725377-1725396 Msa1306760:intron 45.0%
! GAGGGAGAGTATGCCTAAAA+CGG - chr8_4:1725614-1725633 None:intergenic 45.0%
! GTTTTCTGTTTGGGAGAGTG+GGG - chr8_4:1725955-1725974 None:intergenic 45.0%
! CGTTTTCTGTTTGGGAGAGT+GGG - chr8_4:1725956-1725975 None:intergenic 45.0%
!! ATCGTGGTGATGGCACTATA+CGG + chr8_4:1726010-1726029 Msa1306760:intron 45.0%
CTATACGGCCGAGTTAAATC+AGG + chr8_4:1726025-1726044 Msa1306760:intron 45.0%
CCAACGTAAACGAATGACCA+TGG - chr8_4:1726469-1726488 None:intergenic 45.0%
CCATGGTCATTCGTTTACGT+TGG + chr8_4:1726466-1726485 Msa1306760:intron 45.0%
GGTCATTCGTTTACGTTGGA+AGG + chr8_4:1726470-1726489 Msa1306760:intron 45.0%
TTCATGTTTGCAGCATCTGC+TGG + chr8_4:1726947-1726966 Msa1306760:intron 45.0%
!! TTGCCGTGTTACTTCTCTCT+TGG - chr8_4:1726988-1727007 None:intergenic 45.0%
!!! CAATGCTAGCTGTTGGCTTT+TGG + chr8_4:1727432-1727451 Msa1306760:intron 45.0%
GGACAAGATCCAAATTTGGG+GGG - chr8_4:1728512-1728531 None:intergenic 45.0%
TTATGGAAAGCCTCCCTAGT+TGG + chr8_4:1728567-1728586 Msa1306760:intron 45.0%
TTATGGAAAGCCTCCCTAGT+TGG + chr8_4:1728567-1728586 Msa1306760:intron 45.0%
GTAAATGGAGCTTCCAAGAC+AGG - chr8_4:1729086-1729105 None:intergenic 45.0%
TCTTCTGCTGCAGAGGTAAT+GGG - chr8_4:1729529-1729548 None:intergenic 45.0%
CATTTAACCTCTCACTGCAC+AGG - chr8_4:1729687-1729706 None:intergenic 45.0%
GGATCTTGATGAGGAGGTAA+TGG - chr8_4:1729992-1730011 None:intergenic 45.0%
GCCTAAAAGGGATCTTGATG+AGG - chr8_4:1730001-1730020 None:intergenic 45.0%
! GCTTGTTGATGAAGATGGTG+GGG + chr8_4:1730339-1730358 Msa1306760:intron 45.0%
! GCTTGTTGATGAAGATGGTG+GGG + chr8_4:1730339-1730358 Msa1306760:intron 45.0%
GAAAGGAAGGAAGGAAGGAA+CGG + chr8_4:1730446-1730465 Msa1306760:intron 45.0%
AAAGGAAGGAAGGAAGGAAC+GGG + chr8_4:1730447-1730466 Msa1306760:intron 45.0%
CCCACATGCAAATGTTTGCT+TGG + chr8_4:1730703-1730722 Msa1306760:intron 45.0%
CCATTTAAGCACCCAGTAGA+AGG - chr8_4:1731324-1731343 None:intergenic 45.0%
CCTTCTACTGGGTGCTTAAA+TGG + chr8_4:1731321-1731340 Msa1306760:intron 45.0%
CAGAATAGAGGATTGTTGGC+TGG + chr8_4:1731696-1731715 Msa1306760:intron 45.0%
! AATAGAGGATTGTTGGCTGG+AGG + chr8_4:1731699-1731718 Msa1306760:five_prime_UTR 45.0%
GCAGCACTTCAACTACTTGT+TGG + chr8_4:1732845-1732864 Msa1306760:intron 45.0%
GTGAGGGAAGAGAAATGATG+AGG - chr8_4:1733020-1733039 None:intergenic 45.0%
!!! AGTAATTTATTTATTTTTTT+CGG + chr8_4:1724466-1724485 Msa1306760:five_prime_UTR 5.0%
!!! AAACTATAATTTTTTTTTAA+GGG - chr8_4:1725305-1725324 None:intergenic 5.0%
!!! TAAACTATAATTTTTTTTTA+AGG - chr8_4:1725306-1725325 None:intergenic 5.0%
!! AAAATAGTAAAAATATTTAA+TGG - chr8_4:1725439-1725458 None:intergenic 5.0%
!! ATAAAAAAAATCAATAAAAA+AGG - chr8_4:1726530-1726549 None:intergenic 5.0%
!! AATATTAAATTATTTCAAAA+TGG + chr8_4:1727569-1727588 Msa1306760:intron 5.0%
!! ATATTAAATTATTTCAAAAT+GGG + chr8_4:1727570-1727589 Msa1306760:intron 5.0%
!!! TATTTTTTATTTTCATTATT+TGG + chr8_4:1729424-1729443 Msa1306760:intron 5.0%
!!! ATTATACATATTTTTTTATT+TGG - chr8_4:1730529-1730548 None:intergenic 5.0%
!!! TTTTAATAAAAAACATAATT+TGG + chr8_4:1731235-1731254 Msa1306760:intron 5.0%
!!! TAATTTATTTTAAATTTTGT+TGG + chr8_4:1731630-1731649 Msa1306760:intron 5.0%
!!! AAAGAAAATTTATTTTTATT+TGG + chr8_4:1731673-1731692 Msa1306760:intron 5.0%
!!! ATATTTTTTCAAAAAAAATT+AGG + chr8_4:1732490-1732509 Msa1306760:intron 5.0%
!! TATAAATATAAATAATGAAA+AGG + chr8_4:1732728-1732747 Msa1306760:intron 5.0%
!!! ATAAAATTTTAAAATTAAGT+TGG - chr8_4:1733067-1733086 None:intergenic 5.0%
TTCAGGCGAGAGAGAGAAAG+GGG - chr8_4:1724087-1724106 None:intergenic 50.0%
GTTTCAGGCGAGAGAGAGAA+AGG - chr8_4:1724089-1724108 None:intergenic 50.0%
TCGCCGGAAAGATGTAGATC+TGG - chr8_4:1724175-1724194 None:intergenic 50.0%
!! GGAGGTTAGTTTAGTGGTGG+TGG - chr8_4:1724335-1724354 None:intergenic 50.0%
!! TGGGGAGGTTAGTTTAGTGG+TGG - chr8_4:1724338-1724357 None:intergenic 50.0%
CGGCGGTCTGAAAGATCAAT+AGG - chr8_4:1724397-1724416 None:intergenic 50.0%
CCGAGTTCGAATCCAGATGA+AGG - chr8_4:1725379-1725398 None:intergenic 50.0%
! GGAGAGTATGCCTAAAACGG+TGG - chr8_4:1725611-1725630 None:intergenic 50.0%
GATTGGGCTTGACCCAATTC+TGG - chr8_4:1725644-1725663 None:intergenic 50.0%
TACCAGTTCGAGATCGACTG+TGG - chr8_4:1725796-1725815 None:intergenic 50.0%
! GTGGGGAGTGAAGTGTTTGA+AGG - chr8_4:1725938-1725957 None:intergenic 50.0%
! GCGTTTTCTGTTTGGGAGAG+TGG - chr8_4:1725957-1725976 None:intergenic 50.0%
AGGGTTACGAATCGTGGTGA+TGG + chr8_4:1726000-1726019 Msa1306760:intron 50.0%
CCTGAGAGATGAAAGGAGCA+AGG - chr8_4:1726096-1726115 None:intergenic 50.0%
CCTTGCTCCTTTCATCTCTC+AGG + chr8_4:1726093-1726112 Msa1306760:intron 50.0%
AGCATCTGCTGGCAATGAAC+AGG + chr8_4:1726958-1726977 Msa1306760:intron 50.0%
TTGCCTTATAGCAGCTGTGC+AGG + chr8_4:1727138-1727157 Msa1306760:intron 50.0%
GTACCTGCACAGCTGCTATA+AGG - chr8_4:1727144-1727163 None:intergenic 50.0%
CGTGTATGCCTTGCCAACTA+GGG - chr8_4:1728648-1728667 None:intergenic 50.0%
ACGTGTATGCCTTGCCAACT+AGG - chr8_4:1728649-1728668 None:intergenic 50.0%
TAGTTGGCAAGGCATACACG+TGG + chr8_4:1728648-1728667 Msa1306760:intron 50.0%
CTCTTCTGCTGCAGAGGTAA+TGG - chr8_4:1729530-1729549 None:intergenic 50.0%
TACCTCTGCAGCAGAAGAGA+AGG + chr8_4:1729531-1729550 Msa1306760:intron 50.0%
ATCCTTCTCTTCTGCTGCAG+AGG - chr8_4:1729536-1729555 None:intergenic 50.0%
TGGTGGGGATTGTTTCAGCA+AGG + chr8_4:1730246-1730265 Msa1306760:intron 50.0%
TGGTGGGGATTGTTTCAGCA+AGG + chr8_4:1730246-1730265 Msa1306760:intron 50.0%
!! GAAGATGGTGGGGATTGAGA+AGG + chr8_4:1730412-1730431 Msa1306760:intron 50.0%
AAGGAAGGAACGGGATGTGT+TGG + chr8_4:1730456-1730475 Msa1306760:intron 50.0%
TGGTCGGAAATTGGCGGTGA+CGG - chr8_4:1724155-1724174 None:intergenic 55.0%
TGGTGCATCCAAATGGTGGC+CGG - chr8_4:1724315-1724334 None:intergenic 55.0%
! GTGGTGGTGCATCCAAATGG+TGG - chr8_4:1724319-1724338 None:intergenic 55.0%
!! GTGGTGGTGGTGCATCCAAA+TGG - chr8_4:1724322-1724341 None:intergenic 55.0%
!! GAAATGACCTCTGAAGCCGG+CGG - chr8_4:1724414-1724433 None:intergenic 55.0%
!! CCGGAAATGACCTCTGAAGC+CGG - chr8_4:1724417-1724436 None:intergenic 55.0%
CCGGCTTCAGAGGTCATTTC+CGG + chr8_4:1724414-1724433 Msa1306760:five_prime_UTR 55.0%
! TGGTGGGCGACAATATGCTG+GGG - chr8_4:1725770-1725789 None:intergenic 55.0%
!! GTGGTGGGCGACAATATGCT+GGG - chr8_4:1725771-1725790 None:intergenic 55.0%
!! AGATCGACTGTGGTGGTGGT+GGG - chr8_4:1725786-1725805 None:intergenic 55.0%
TTCGAGATCGACTGTGGTGG+TGG - chr8_4:1725790-1725809 None:intergenic 55.0%
CAGTTCGAGATCGACTGTGG+TGG - chr8_4:1725793-1725812 None:intergenic 55.0%
CACCACAGTCGATCTCGAAC+TGG + chr8_4:1725791-1725810 Msa1306760:intron 55.0%
GAAAGCCTCCCTAGTTGGCA+AGG + chr8_4:1728572-1728591 Msa1306760:intron 55.0%
GGTGGATGCCTTGCCAACTA+GGG - chr8_4:1728583-1728602 None:intergenic 55.0%
! TGGTGGATGCCTTGCCAACT+AGG - chr8_4:1728584-1728603 None:intergenic 55.0%
GAAAGCCTCCCTAGTTGGCA+AGG + chr8_4:1728572-1728591 Msa1306760:intron 55.0%
GTATGCCTTGCCAACTAGGG+AGG - chr8_4:1728645-1728664 None:intergenic 55.0%
GTTGTGCGTCTGATCCCCAT+TGG + chr8_4:1729039-1729058 Msa1306760:intron 55.0%
! TTGGCGGTGACGGTGGTTCA+TGG - chr8_4:1724145-1724164 None:intergenic 60.0%
! TCGGAAATTGGCGGTGACGG+TGG - chr8_4:1724152-1724171 None:intergenic 60.0%
!! TTTCAGACCGCCGGCTTCAG+AGG + chr8_4:1724404-1724423 Msa1306760:five_prime_UTR 60.0%
TCATTTCCGGCCAACCACCG+CGG + chr8_4:1724427-1724446 Msa1306760:five_prime_UTR 60.0%
! CAATATGCTGGGGGTGGTGG+TGG - chr8_4:1725760-1725779 None:intergenic 60.0%
CGACAATATGCTGGGGGTGG+TGG - chr8_4:1725763-1725782 None:intergenic 60.0%
GGTGGGCGACAATATGCTGG+GGG - chr8_4:1725769-1725788 None:intergenic 60.0%
!! GGTGGTGGGCGACAATATGC+TGG - chr8_4:1725772-1725791 None:intergenic 60.0%
!! GAGATCGACTGTGGTGGTGG+TGG - chr8_4:1725787-1725806 None:intergenic 60.0%
GGATGCCTTGCCAACTAGGG+AGG - chr8_4:1728580-1728599 None:intergenic 60.0%
! GAGGATTGTTGGCTGGAGGC+AGG + chr8_4:1731703-1731722 Msa1306760:five_prime_UTR 60.0%
! GCGGTGACGGTGGTTCATGG+TGG - chr8_4:1724142-1724161 None:intergenic 65.0%
ACCGCGGACAACCACCGTAC+CGG + chr8_4:1724443-1724462 Msa1306760:five_prime_UTR 65.0%
TCCGGTACGGTGGTTGTCCG+CGG - chr8_4:1724447-1724466 None:intergenic 65.0%
ACTAGTGTGCGACCCGTGCG+CGG + chr8_4:1724553-1724572 Msa1306760:intron 65.0%
TTTACCCGTGCGTCCGCGCA+CGG - chr8_4:1724569-1724588 None:intergenic 65.0%
!! TATGCTGGGGGTGGTGGTGG+CGG - chr8_4:1725757-1725776 None:intergenic 65.0%
GGGCGACAATATGCTGGGGG+TGG - chr8_4:1725766-1725785 None:intergenic 65.0%
GGTTGTCCGCGGTGGTTGGC+CGG - chr8_4:1724436-1724455 None:intergenic 70.0%
CGGTGGTTGTCCGCGGTGGT+TGG - chr8_4:1724440-1724459 None:intergenic 70.0%
GGTACGGTGGTTGTCCGCGG+TGG - chr8_4:1724444-1724463 None:intergenic 70.0%
TTACCCGTGCGTCCGCGCAC+GGG - chr8_4:1724568-1724587 None:intergenic 70.0%
!! GGTGGTGGTGGCGGCTGTTG+AGG - chr8_4:1725748-1725767 None:intergenic 70.0%
! GCGACCCGTGCGCGGACGCA+CGG + chr8_4:1724561-1724580 Msa1306760:intron 80.0%
! CGACCCGTGCGCGGACGCAC+GGG + chr8_4:1724562-1724581 Msa1306760:intron 80.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 1724040 1733174 1724040 ID=Msa1306760;Name=Msa1306760
chr8_4 mRNA 1724040 1733174 1724040 ID=Msa1306760-mRNA-1;Parent=Msa1306760;Name=Msa1306760-mRNA-1;_AED=0.39;_eAED=0.39;_QI=750|0.75|0.8|1|0.5|0.4|5|162|105
chr8_4 exon 1724040 1724511 1724040 ID=Msa1306760-mRNA-1:exon:556;Parent=Msa1306760-mRNA-1
chr8_4 exon 1731699 1731827 1731699 ID=Msa1306760-mRNA-1:exon:557;Parent=Msa1306760-mRNA-1
chr8_4 exon 1732007 1732201 1732007 ID=Msa1306760-mRNA-1:exon:558;Parent=Msa1306760-mRNA-1
chr8_4 exon 1732283 1732390 1732283 ID=Msa1306760-mRNA-1:exon:559;Parent=Msa1306760-mRNA-1
chr8_4 exon 1732849 1733174 1732849 ID=Msa1306760-mRNA-1:exon:560;Parent=Msa1306760-mRNA-1
chr8_4 five_prime_UTR 1724040 1724511 1724040 ID=Msa1306760-mRNA-1:five_prime_utr;Parent=Msa1306760-mRNA-1
chr8_4 five_prime_UTR 1731699 1731827 1731699 ID=Msa1306760-mRNA-1:five_prime_utr;Parent=Msa1306760-mRNA-1
chr8_4 five_prime_UTR 1732007 1732155 1732007 ID=Msa1306760-mRNA-1:five_prime_utr;Parent=Msa1306760-mRNA-1
chr8_4 CDS 1732156 1732201 1732156 ID=Msa1306760-mRNA-1:cds;Parent=Msa1306760-mRNA-1
chr8_4 CDS 1732283 1732390 1732283 ID=Msa1306760-mRNA-1:cds;Parent=Msa1306760-mRNA-1
chr8_4 CDS 1732849 1733012 1732849 ID=Msa1306760-mRNA-1:cds;Parent=Msa1306760-mRNA-1
chr8_4 three_prime_UTR 1733013 1733174 1733013 ID=Msa1306760-mRNA-1:three_prime_utr;Parent=Msa1306760-mRNA-1
Gene Sequence

>Msa1306760

ATGAAGATAAATACAAAAAATGAAGATCAGAAAAAACAAAGAAGAGGACCACTTCAACTTTCAGTTGCAAAAGGAACAACTTTCAAGTTTCTAGAGAACATCTTTCAAGAGAAGATGAAGATTAGAAAGTTTGAAAACCTAGCAAATAAAAGTGCACTTCAACTACTTGTTGGGTTGAGTAAAGAACTTTCAAGTTTCCTGCAAAATAAAATTGAAGAGAAGATGGAGATTAGAAAGTGTGAAAACCTACAAAATAGAAGTGAAGAGTGGAAGATGAAGATTAGAAAGTTTGAAAAGAAGAAAAAATATAGACCTTGA
Protein sequence

>Msa1306760

MKINTKNEDQKKQRRGPLQLSVAKGTTFKFLENIFQEKMKIRKFENLANKSALQLLVGLSKELSSFLQNKIEEKMEIRKCENLQNRSEEWKMKIRKFEKKKKYRP*