Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366970 | XP_039683558.1 | 79.487 | 78 | 16 | 0 | 2 | 79 | 115 | 192 | 2.45e-31 | 118 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366970 | A0A396H1H4 | 79.487 | 78 | 16 | 0 | 2 | 79 | 115 | 192 | 1.17e-31 | 118 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002230 | Msa1366970 | 0.813281 | 2.771596e-51 | -8.615850e-47 |
| Msa0002240 | Msa1366970 | 0.833974 | 3.918090e-56 | -8.615850e-47 |
| Msa0003890 | Msa1366970 | 0.816504 | 5.344436e-52 | -8.615850e-47 |
| Msa0011660 | Msa1366970 | -0.814176 | 1.760272e-51 | -8.615850e-47 |
| Msa0014710 | Msa1366970 | 0.804153 | 2.476109e-49 | -8.615850e-47 |
| Msa0031520 | Msa1366970 | 0.851127 | 1.086164e-60 | -8.615850e-47 |
| Msa1345220 | Msa1366970 | 0.816998 | 4.141763e-52 | -8.615850e-47 |
| Msa1366810 | Msa1366970 | 0.880131 | 7.067553e-70 | -8.615850e-47 |
| Msa1366970 | Msa1384240 | 0.806659 | 7.386862e-50 | -8.615850e-47 |
| Msa1366970 | Msa1388160 | 0.901536 | 2.426166e-78 | -8.615850e-47 |
| Msa1366970 | Msa1412530 | 0.848451 | 6.077677e-60 | -8.615850e-47 |
| Msa1366970 | Msa1412540 | 0.804360 | 2.242420e-49 | -8.615850e-47 |
| Msa1366970 | Msa1437270 | 0.807395 | 5.162727e-50 | -8.615850e-47 |
| Msa1366970 | Msa1458750 | 0.849346 | 3.431026e-60 | -8.615850e-47 |
| Msa0602980 | Msa1366970 | 0.849660 | 2.803158e-60 | -8.615850e-47 |
| Msa0632740 | Msa1366970 | 0.801974 | 6.989927e-49 | -8.615850e-47 |
| Msa0645300 | Msa1366970 | 0.817137 | 3.853374e-52 | -8.615850e-47 |
| Msa0701010 | Msa1366970 | 0.832469 | 9.285571e-56 | -8.615850e-47 |
| Msa0739280 | Msa1366970 | 0.827888 | 1.219851e-54 | -8.615850e-47 |
| Msa0748970 | Msa1366970 | 0.842949 | 1.891309e-58 | -8.615850e-47 |
| Msa0750650 | Msa1366970 | 0.835976 | 1.226311e-56 | -8.615850e-47 |
| Msa0756460 | Msa1366970 | 0.806165 | 9.392094e-50 | -8.615850e-47 |
| Msa0767750 | Msa1366970 | 0.842823 | 2.043006e-58 | -8.615850e-47 |
| Msa0767810 | Msa1366970 | 0.839557 | 1.475222e-57 | -8.615850e-47 |
| Msa0768290 | Msa1366970 | 0.860188 | 2.458399e-63 | -8.615850e-47 |
| Msa0768370 | Msa1366970 | 0.871772 | 5.295642e-67 | -8.615850e-47 |
| Msa0773180 | Msa1366970 | 0.824718 | 6.933182e-54 | -8.615850e-47 |
| Msa0804810 | Msa1366970 | 0.803898 | 2.797600e-49 | -8.615850e-47 |
| Msa0820850 | Msa1366970 | 0.800840 | 1.193037e-48 | -8.615850e-47 |
| Msa0824590 | Msa1366970 | 0.800378 | 1.482369e-48 | -8.615850e-47 |
| Msa0826320 | Msa1366970 | 0.810957 | 8.902707e-51 | -8.615850e-47 |
| Msa0832040 | Msa1366970 | 0.813847 | 2.080270e-51 | -8.615850e-47 |
| Msa0131770 | Msa1366970 | 0.825478 | 4.587182e-54 | -8.615850e-47 |
| Msa0150840 | Msa1366970 | 0.816301 | 5.932989e-52 | -8.615850e-47 |
| Msa0171140 | Msa1366970 | 0.818055 | 2.393676e-52 | -8.615850e-47 |
| Msa0171540 | Msa1366970 | 0.804329 | 2.276054e-49 | -8.615850e-47 |
| Msa0180500 | Msa1366970 | 0.847394 | 1.189329e-59 | -8.615850e-47 |
| Msa0194410 | Msa1366970 | 0.801907 | 7.215787e-49 | -8.615850e-47 |
| Msa0198910 | Msa1366970 | 0.805340 | 1.399408e-49 | -8.615850e-47 |
| Msa0224330 | Msa1366970 | 0.815353 | 9.656329e-52 | -8.615850e-47 |
| Msa0234270 | Msa1366970 | 0.813838 | 2.089574e-51 | -8.615850e-47 |
| Msa1227730 | Msa1366970 | 0.839960 | 1.159205e-57 | -8.615850e-47 |
| Msa1232740 | Msa1366970 | 0.826341 | 2.861559e-54 | -8.615850e-47 |
| Msa1254730 | Msa1366970 | 0.809326 | 1.999863e-50 | -8.615850e-47 |
| Msa1276600 | Msa1366970 | 0.856671 | 2.748741e-62 | -8.615850e-47 |
| Msa1276890 | Msa1366970 | 0.820209 | 7.742115e-53 | -8.615850e-47 |
| Msa1286800 | Msa1366970 | 0.841069 | 5.941373e-58 | -8.615850e-47 |
| Msa1287190 | Msa1366970 | 0.817305 | 3.533750e-52 | -8.615850e-47 |
| Msa1302850 | Msa1366970 | 0.812413 | 4.292673e-51 | -8.615850e-47 |
| Msa1306760 | Msa1366970 | 0.837607 | 4.703817e-57 | -8.615850e-47 |
| Msa1306860 | Msa1366970 | 0.808943 | 2.416192e-50 | -8.615850e-47 |
| Msa1313320 | Msa1366970 | 0.829002 | 6.566608e-55 | -8.615850e-47 |
| Msa0402790 | Msa1366970 | 0.811289 | 7.542508e-51 | -8.615850e-47 |
| Msa0413410 | Msa1366970 | 0.807270 | 5.486055e-50 | -8.615850e-47 |
| Msa0416650 | Msa1366970 | 0.804804 | 1.811552e-49 | -8.615850e-47 |
| Msa0424320 | Msa1366970 | 0.832578 | 8.724740e-56 | -8.615850e-47 |
| Msa0425790 | Msa1366970 | 0.843591 | 1.275542e-58 | -8.615850e-47 |
| Msa0430920 | Msa1366970 | 0.808003 | 3.833726e-50 | -8.615850e-47 |
| Msa0465200 | Msa1366970 | 0.813546 | 2.423504e-51 | -8.615850e-47 |
| Msa1000860 | Msa1366970 | 0.840750 | 7.205515e-58 | -8.615850e-47 |
| Msa1000890 | Msa1366970 | 0.860792 | 1.612881e-63 | -8.615850e-47 |
| Msa1000900 | Msa1366970 | 0.847349 | 1.223024e-59 | -8.615850e-47 |
| Msa1009070 | Msa1366970 | 0.814294 | 1.657629e-51 | -8.615850e-47 |
| Msa1035660 | Msa1366970 | 0.814213 | 1.727436e-51 | -8.615850e-47 |
| Msa1037420 | Msa1366970 | 0.811440 | 6.992606e-51 | -8.615850e-47 |
| Msa1058710 | Msa1366970 | 0.812080 | 5.075382e-51 | -8.615850e-47 |
| Msa1075540 | Msa1366970 | 0.824329 | 8.563829e-54 | -8.615850e-47 |
| Msa0243460 | Msa1366970 | 0.806614 | 7.551328e-50 | -8.615850e-47 |
| Msa0251640 | Msa1366970 | 0.838107 | 3.499738e-57 | -8.615850e-47 |
| Msa0255080 | Msa1366970 | 0.807537 | 4.816364e-50 | -8.615850e-47 |
| Msa0259210 | Msa1366970 | 0.801307 | 9.577827e-49 | -8.615850e-47 |
| Msa0271770 | Msa1366970 | 0.833944 | 3.986271e-56 | -8.615850e-47 |
| Msa0278110 | Msa1366970 | 0.851475 | 8.665085e-61 | -8.615850e-47 |
| Msa0278190 | Msa1366970 | 0.804588 | 2.010298e-49 | -8.615850e-47 |
| Msa0298460 | Msa1366970 | 0.827865 | 1.235627e-54 | -8.615850e-47 |
| Msa0317520 | Msa1366970 | 0.801410 | 9.121517e-49 | -8.615850e-47 |
| Msa0336320 | Msa1366970 | 0.811288 | 7.544622e-51 | -8.615850e-47 |
| Msa0342750 | Msa1366970 | 0.859891 | 3.022049e-63 | -8.615850e-47 |
| Msa0343850 | Msa1366970 | 0.805170 | 1.518753e-49 | -8.615850e-47 |
| Msa0353540 | Msa1366970 | 0.807091 | 5.988428e-50 | -8.615850e-47 |
| Msa1108690 | Msa1366970 | 0.841276 | 5.242400e-58 | -8.615850e-47 |
| Msa1113170 | Msa1366970 | 0.814195 | 1.743069e-51 | -8.615850e-47 |
| Msa1115100 | Msa1366970 | 0.801731 | 7.839521e-49 | -8.615850e-47 |
| Msa1119930 | Msa1366970 | 0.805826 | 1.106450e-49 | -8.615850e-47 |
| Msa1121480 | Msa1366970 | 0.809236 | 2.090442e-50 | -8.615850e-47 |
| Msa1121490 | Msa1366970 | 0.820173 | 7.887907e-53 | -8.615850e-47 |
| Msa1137570 | Msa1366970 | 0.825890 | 3.661601e-54 | -8.615850e-47 |
| Msa1156020 | Msa1366970 | 0.805352 | 1.391597e-49 | -8.615850e-47 |
| Msa1178180 | Msa1366970 | 0.802902 | 4.499887e-49 | -8.615850e-47 |
| Msa0490140 | Msa1366970 | 0.833364 | 5.565945e-56 | -8.615850e-47 |
| Msa0514320 | Msa1366970 | 0.806644 | 7.442557e-50 | -8.615850e-47 |
| Msa0519000 | Msa1366970 | 0.821683 | 3.544850e-53 | -8.615850e-47 |
| Msa0523940 | Msa1366970 | 0.841536 | 4.476615e-58 | -8.615850e-47 |
| Msa0529040 | Msa1366970 | 0.806516 | 7.919854e-50 | -8.615850e-47 |
| Msa0530400 | Msa1366970 | 0.800840 | 1.193535e-48 | -8.615850e-47 |
| Msa0539530 | Msa1366970 | -0.801742 | 7.799926e-49 | -8.615850e-47 |
| Msa0545700 | Msa1366970 | 0.831637 | 1.490801e-55 | -8.615850e-47 |
| Msa0844410 | Msa1366970 | 0.831753 | 1.395910e-55 | -8.615850e-47 |
| Msa0844420 | Msa1366970 | 0.861035 | 1.360589e-63 | -8.615850e-47 |
| Msa0844440 | Msa1366970 | 0.819215 | 1.305633e-52 | -8.615850e-47 |
| Msa0844450 | Msa1366970 | 0.820425 | 6.904695e-53 | -8.615850e-47 |
| Msa0844470 | Msa1366970 | 0.842277 | 2.852269e-58 | -8.615850e-47 |
| Msa0844490 | Msa1366970 | 0.868259 | 7.456708e-66 | -8.615850e-47 |
| Msa0849030 | Msa1366970 | 0.837633 | 4.633729e-57 | -8.615850e-47 |
| Msa0857640 | Msa1366970 | 0.823382 | 1.427366e-53 | -8.615850e-47 |
| Msa0877250 | Msa1366970 | 0.831209 | 1.900246e-55 | -8.615850e-47 |
| Msa0888130 | Msa1366970 | 0.808286 | 3.336811e-50 | -8.615850e-47 |
| Msa0897330 | Msa1366970 | 0.817581 | 3.061829e-52 | -8.615850e-47 |
| Msa0899430 | Msa1366970 | 0.808265 | 3.372313e-50 | -8.615850e-47 |
| Msa0899450 | Msa1366970 | 0.800023 | 1.750311e-48 | -8.615850e-47 |
| Msa0900910 | Msa1366970 | 0.819402 | 1.183441e-52 | -8.615850e-47 |
| Msa0901070 | Msa1366970 | 0.852140 | 5.610030e-61 | -8.615850e-47 |
| Msa0903620 | Msa1366970 | 0.800750 | 1.244802e-48 | -8.615850e-47 |
| Msa0914270 | Msa1366970 | 0.813325 | 2.710234e-51 | -8.615850e-47 |
| Msa0914760 | Msa1366970 | 0.800061 | 1.719486e-48 | -8.615850e-47 |
| Msa0927380 | Msa1366970 | 0.839103 | 1.935689e-57 | -8.615850e-47 |
| Msa0936300 | Msa1366970 | 0.821203 | 4.573784e-53 | -8.615850e-47 |
| Msa0948320 | Msa1366970 | 0.854140 | 1.500582e-61 | -8.615850e-47 |
| Msa0958550 | Msa1366970 | 0.801099 | 1.056238e-48 | -8.615850e-47 |
| Msa0964900 | Msa1366970 | 0.803798 | 2.935811e-49 | -8.615850e-47 |
| Msa0966750 | Msa1366970 | 0.855184 | 7.478943e-62 | -8.615850e-47 |
| Msa0969520 | Msa1366970 | 0.810278 | 1.247745e-50 | -8.615850e-47 |
| Msa0969810 | Msa1366970 | 0.856871 | 2.399037e-62 | -8.615850e-47 |
| Msa0973100 | Msa1366970 | 0.818807 | 1.616361e-52 | -8.615850e-47 |
| Msa0974070 | Msa1366970 | 0.815665 | 8.229338e-52 | -8.615850e-47 |
| Msa0974080 | Msa1366970 | 0.852448 | 4.585534e-61 | -8.615850e-47 |
| Msa0976670 | Msa1366970 | 0.812871 | 3.408938e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1366970 | MtrunA17_Chr7g0225251 | 79.487 | 78 | 16 | 0 | 2 | 79 | 115 | 192 | 2.26e-35 | 118 |
| Msa1366970 | MtrunA17_Chr7g0225371 | 79.487 | 78 | 16 | 0 | 2 | 79 | 176 | 253 | 7.21e-35 | 119 |
| Msa1366970 | MtrunA17_Chr7g0225341 | 81.429 | 70 | 12 | 1 | 2 | 71 | 49 | 117 | 3.99e-33 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCATAAGGAGGATGCTTC+AGG | 0.377294 | tig0018509:-246378 | Msa1366970:CDS |
| ACTCCTTCGTCTATTAGCTT+TGG | 0.380513 | tig0018509:-246282 | Msa1366970:CDS |
| TGCTAAAACAAGGTCTTCTA+TGG | 0.383482 | tig0018509:+246332 | None:intergenic |
| ATGACATGCCTTTGGTGTTA+CGG | 0.399250 | tig0018509:+246256 | None:intergenic |
| AGGGACAAATGACATGCCTT+TGG | 0.478422 | tig0018509:+246248 | None:intergenic |
| CGAGCTGTTCAGTGTGGAAA+GGG | 0.479849 | tig0018509:-246225 | Msa1366970:CDS |
| GTGTGGAAAGGGATCAAACT+CGG | 0.545538 | tig0018509:-246214 | Msa1366970:CDS |
| TCGAGCTGTTCAGTGTGGAA+AGG | 0.580780 | tig0018509:-246226 | Msa1366970:CDS |
| AGTCTCTAGAGTGAATACTT+TGG | 0.586283 | tig0018509:-246310 | Msa1366970:CDS |
| TTTGGTTGCCGTAACACCAA+AGG | 0.586816 | tig0018509:-246264 | Msa1366970:CDS |
| CTCTAGAGACTGCTAAAACA+AGG | 0.590843 | tig0018509:+246322 | None:intergenic |
| TGCTTCAGGAATGAATGAAG+TGG | 0.597871 | tig0018509:-246364 | Msa1366970:CDS |
| TTTCCACACTGAACAGCTCG+AGG | 0.606690 | tig0018509:+246228 | None:intergenic |
| TGTGGAAAGGGATCAAACTC+GGG | 0.608253 | tig0018509:-246213 | Msa1366970:CDS |
| TTCCACACTGAACAGCTCGA+GGG | 0.645561 | tig0018509:+246229 | None:intergenic |
| GTCCCTCGAGCTGTTCAGTG+TGG | 0.654976 | tig0018509:-246231 | Msa1366970:CDS |
| CAACCAAAGCTAATAGACGA+AGG | 0.701927 | tig0018509:+246279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAAAACATTCTCATAAGG+AGG | - | tig0018509:246208-246227 | Msa1366970:CDS | 30.0% | |
| GCGCAAAAAACATTCTCATA+AGG | - | tig0018509:246205-246224 | Msa1366970:CDS | 35.0% | |
| TGCTAAAACAAGGTCTTCTA+TGG | + | tig0018509:246267-246286 | None:intergenic | 35.0% | |
| !! | AGTCTCTAGAGTGAATACTT+TGG | - | tig0018509:246286-246305 | Msa1366970:CDS | 35.0% |
| TGCTTCAGGAATGAATGAAG+TGG | - | tig0018509:246232-246251 | Msa1366970:CDS | 40.0% | |
| ! | CTCTAGAGACTGCTAAAACA+AGG | + | tig0018509:246277-246296 | None:intergenic | 40.0% |
| ! | ACTCCTTCGTCTATTAGCTT+TGG | - | tig0018509:246314-246333 | Msa1366970:CDS | 40.0% |
| CAACCAAAGCTAATAGACGA+AGG | + | tig0018509:246320-246339 | None:intergenic | 40.0% | |
| !! | ATGACATGCCTTTGGTGTTA+CGG | + | tig0018509:246343-246362 | None:intergenic | 40.0% |
| TCTCATAAGGAGGATGCTTC+AGG | - | tig0018509:246218-246237 | Msa1366970:CDS | 45.0% | |
| TTTGGTTGCCGTAACACCAA+AGG | - | tig0018509:246332-246351 | Msa1366970:CDS | 45.0% | |
| AGGGACAAATGACATGCCTT+TGG | + | tig0018509:246351-246370 | None:intergenic | 45.0% | |
| GTGTGGAAAGGGATCAAACT+CGG | - | tig0018509:246382-246401 | Msa1366970:CDS | 45.0% | |
| TGTGGAAAGGGATCAAACTC+GGG | - | tig0018509:246383-246402 | Msa1366970:CDS | 45.0% | |
| TTCCACACTGAACAGCTCGA+GGG | + | tig0018509:246370-246389 | None:intergenic | 50.0% | |
| TTTCCACACTGAACAGCTCG+AGG | + | tig0018509:246371-246390 | None:intergenic | 50.0% | |
| TCGAGCTGTTCAGTGTGGAA+AGG | - | tig0018509:246370-246389 | Msa1366970:CDS | 50.0% | |
| CGAGCTGTTCAGTGTGGAAA+GGG | - | tig0018509:246371-246390 | Msa1366970:CDS | 50.0% | |
| GTCCCTCGAGCTGTTCAGTG+TGG | - | tig0018509:246365-246384 | Msa1366970:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018509 | gene | 246188 | 246430 | 246188 | ID=Msa1366970;Name=Msa1366970 |
| tig0018509 | mRNA | 246188 | 246430 | 246188 | ID=Msa1366970-mRNA-1;Parent=Msa1366970;Name=Msa1366970-mRNA-1;_AED=0.31;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|80 |
| tig0018509 | exon | 246188 | 246430 | 246188 | ID=Msa1366970-mRNA-1:exon:15284;Parent=Msa1366970-mRNA-1 |
| tig0018509 | CDS | 246188 | 246430 | 246188 | ID=Msa1366970-mRNA-1:cds;Parent=Msa1366970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
