AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0767750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGACAAATCGCTTGATTT+TGG 0.221118 5_2:+31523288 Msa0767750:five_prime_UTR
TTCTTTCTTAGCATCTTTCT+TGG 0.275859 5_2:-31523023 None:intergenic
GCTTGCTTCATTGAGTTAGA+AGG 0.322805 5_2:-31522941 None:intergenic
TGTGAATACAGTGTCTCTTC+TGG 0.330987 5_2:-31523085 None:intergenic
GAATGTGTTGAATAGGTTAA+TGG 0.333245 5_2:+31523165 Msa0767750:five_prime_UTR
GGCAAGAAACAATTTCTTCA+TGG 0.365166 5_2:-31525614 None:intergenic
TACAGTGTCTCTTCTGGCCT+CGG 0.375247 5_2:-31523079 None:intergenic
GGGATCGGAGAAGGAAGAAT+TGG 0.406847 5_2:+31523198 Msa0767750:five_prime_UTR
GGCTGACACGGATTGAGATT+CGG 0.451832 5_2:-31522896 None:intergenic
TAGATATGAATGTGTTGAAT+AGG 0.453928 5_2:+31523158 Msa0767750:five_prime_UTR
TGAGTCTGCAGCGGCGGCGG+CGG 0.455416 5_2:-31522972 None:intergenic
TGAACATACTTCCATTCTTG+TGG 0.480056 5_2:+31525641 Msa0767750:CDS
TGATGAGTCTGCAGCGGCGG+CGG 0.481622 5_2:-31522975 None:intergenic
GGGGAATGTAGTTATCAAAT+GGG 0.485794 5_2:+31523124 Msa0767750:five_prime_UTR
CAAGAATGGAAGTATGTTCA+AGG 0.487778 5_2:-31525638 None:intergenic
CATCAAACCGGCCGCGTAAG+AGG 0.488792 5_2:+31522993 Msa0767750:five_prime_UTR
AGAAGGAAGAATTGGAAAGA+AGG 0.489922 5_2:+31523206 Msa0767750:five_prime_UTR
TCAAGAGTTGAAACTGTTTG+TGG 0.491313 5_2:+31523246 Msa0767750:five_prime_UTR
AATGGAATCGATTGAGGGAT+CGG 0.493309 5_2:+31523183 Msa0767750:five_prime_UTR
GGCTTTATAGTCCACAAGAA+TGG 0.495039 5_2:-31525652 None:intergenic
CTTGCTTCATTGAGTTAGAA+GGG 0.508767 5_2:-31522940 None:intergenic
AGGAAGAATTGGAAAGAAGG+TGG 0.531844 5_2:+31523209 Msa0767750:five_prime_UTR
CCGCTGCAGACTCATCAAAC+CGG 0.534109 5_2:+31522981 Msa0767750:five_prime_UTR
ATCGATTGAGGGATCGGAGA+AGG 0.536386 5_2:+31523189 Msa0767750:five_prime_UTR
TAGGTTAATGGAATCGATTG+AGG 0.553633 5_2:+31523177 Msa0767750:five_prime_UTR
TAAAGCGCCTCTTACGCGGC+CGG 0.553884 5_2:-31523000 None:intergenic
AGGTTAATGGAATCGATTGA+GGG 0.560272 5_2:+31523178 Msa0767750:five_prime_UTR
AGAAGAGACACTGTATTCAC+AGG 0.572182 5_2:+31523087 Msa0767750:five_prime_UTR
CACAGGAAGAATTTGTGAAG+GGG 0.572656 5_2:+31523104 Msa0767750:five_prime_UTR
GTTTGATGAGTCTGCAGCGG+CGG 0.580694 5_2:-31522978 None:intergenic
TTCACAGGAAGAATTTGTGA+AGG 0.593335 5_2:+31523102 Msa0767750:five_prime_UTR
TCACAGGAAGAATTTGTGAA+GGG 0.599562 5_2:+31523103 Msa0767750:five_prime_UTR
AGAGTTGAAACTGTTTGTGG+AGG 0.602889 5_2:+31523249 Msa0767750:five_prime_UTR
TGCTTCATTGAGTTAGAAGG+GGG 0.603394 5_2:-31522938 None:intergenic
CGTCTTAGACACGAAGCATG+AGG 0.614770 5_2:+31523326 Msa0767750:exon
ACAGGAAGAATTTGTGAAGG+GGG 0.620371 5_2:+31523105 Msa0767750:five_prime_UTR
TTGCTTCATTGAGTTAGAAG+GGG 0.637305 5_2:-31522939 None:intergenic
TTGGTAAAGCGCCTCTTACG+CGG 0.641927 5_2:-31523004 None:intergenic
CCGGTTTGATGAGTCTGCAG+CGG 0.651993 5_2:-31522981 None:intergenic
AAAATGCTGCTAAGAAACCG+AGG 0.699686 5_2:+31523062 Msa0767750:five_prime_UTR
GAATGGAAGTATGTTCAAGG+AGG 0.727529 5_2:-31525635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTGTTATGTATTAT+TGG + chr5_2:31525055-31525074 Msa0767750:intron 10.0%
!!! TATTTTTGTTATGTATTATT+GGG + chr5_2:31525056-31525075 Msa0767750:intron 10.0%
!!! TTAGATTTATACTTATTTTG+TGG + chr5_2:31523399-31523418 Msa0767750:intron 15.0%
!! AACAAGAAAAGTTAATAATA+AGG - chr5_2:31524301-31524320 None:intergenic 15.0%
!!! ATTAACTTTTCTTGTTTATA+TGG + chr5_2:31524305-31524324 Msa0767750:intron 15.0%
!!! AAAACATGTTGATTTATTAA+AGG + chr5_2:31524501-31524520 Msa0767750:intron 15.0%
!!! CATTTATATCTTTTTAAATC+TGG + chr5_2:31524801-31524820 Msa0767750:intron 15.0%
!! TAATATAAGCTGATACTTTA+TGG + chr5_2:31523376-31523395 Msa0767750:intron 20.0%
!!! ACATTATTGATTTATCTCTT+TGG + chr5_2:31523995-31524014 Msa0767750:intron 20.0%
!! GAAATAGTAAAGTAATGTAA+AGG + chr5_2:31524083-31524102 Msa0767750:intron 20.0%
!!! GTCTACTTTTTTTGTTTATA+TGG + chr5_2:31524340-31524359 Msa0767750:intron 20.0%
!!! TATATCAGTTTTATGTAATC+TGG + chr5_2:31524643-31524662 Msa0767750:intron 20.0%
!!! ATTTTAGAATGAATCCTTTA+CGG + chr5_2:31524711-31524730 Msa0767750:intron 20.0%
!! ATTCATAGCAAACTATATTA+CGG + chr5_2:31524893-31524912 Msa0767750:intron 20.0%
!! TTCATATAATCATAGTTATG+AGG + chr5_2:31525442-31525461 Msa0767750:intron 20.0%
! TAGATATGAATGTGTTGAAT+AGG + chr5_2:31523158-31523177 Msa0767750:five_prime_UTR 25.0%
!!! TGAACATGTTTTGTTTGATA+TGG + chr5_2:31523683-31523702 Msa0767750:intron 25.0%
! TTGATATGAACATAAGAATG+TGG + chr5_2:31523732-31523751 Msa0767750:intron 25.0%
!! TTAGTGAAACTTTACCTTTT+AGG + chr5_2:31523767-31523786 Msa0767750:intron 25.0%
!! TAGTGAAACTTTACCTTTTA+GGG + chr5_2:31523768-31523787 Msa0767750:intron 25.0%
!! GATTTATCTCTTTGGAAATT+AGG + chr5_2:31524003-31524022 Msa0767750:intron 25.0%
! ATGTAAAGGATTAGACATAA+AGG + chr5_2:31524097-31524116 Msa0767750:intron 25.0%
! ATAATCAAGATAACTGATTG+TGG + chr5_2:31524257-31524276 Msa0767750:intron 25.0%
! GTTTATATGGTTGTATGAAT+TGG + chr5_2:31524318-31524337 Msa0767750:intron 25.0%
!! AAGTGAAATGTTTTGTCAAT+TGG - chr5_2:31524392-31524411 None:intergenic 25.0%
! ATATTGCGTAAAAACTATGT+TGG - chr5_2:31524455-31524474 None:intergenic 25.0%
!! ACATAGTTTTTACGCAATAT+TGG + chr5_2:31524455-31524474 Msa0767750:intron 25.0%
!!! TTTCTTTTCAATCTGTTTCT+GGG + chr5_2:31524524-31524543 Msa0767750:intron 25.0%
! GACTGCATTACTTAATTATT+TGG + chr5_2:31524546-31524565 Msa0767750:intron 25.0%
! TATAACAATCTACGACTATT+TGG - chr5_2:31524627-31524646 None:intergenic 25.0%
! AAAAGAACCTGTTATTAAGA+AGG - chr5_2:31524777-31524796 None:intergenic 25.0%
!! TCTTAATAACAGGTTCTTTT+AGG + chr5_2:31524777-31524796 Msa0767750:intron 25.0%
!! CTTAATAACAGGTTCTTTTA+GGG + chr5_2:31524778-31524797 Msa0767750:intron 25.0%
!! CTTTTTAAATCTGGATTGAT+AGG + chr5_2:31524810-31524829 Msa0767750:intron 25.0%
! TAAATGTATGCTAAGTTTAC+TGG + chr5_2:31524842-31524861 Msa0767750:intron 25.0%
! TTCATAGCAAACTATATTAC+GGG + chr5_2:31524894-31524913 Msa0767750:intron 25.0%
! AACTATATTACGGGTTTAAA+AGG + chr5_2:31524903-31524922 Msa0767750:intron 25.0%
!!! GTATACCAAAGAGTTTTTTT+GGG + chr5_2:31525078-31525097 Msa0767750:intron 25.0%
! TGTGTTAATTGTATTCTTGT+TGG + chr5_2:31525113-31525132 Msa0767750:intron 25.0%
!!! AATTGTATTCTTGTTGGTTT+TGG + chr5_2:31525119-31525138 Msa0767750:intron 25.0%
! ACAATTTGTAGAACAGATTA+AGG - chr5_2:31525154-31525173 None:intergenic 25.0%
!!! TGGTAATTTTTGGAAGATTT+TGG + chr5_2:31525274-31525293 Msa0767750:intron 25.0%
!! TGTGGTACAACTTAAATTTT+GGG + chr5_2:31525394-31525413 Msa0767750:intron 25.0%
!!! TACAACTTAAATTTTGGGTT+GGG + chr5_2:31525399-31525418 Msa0767750:intron 25.0%
! TATAATCATAGTTATGAGGT+TGG + chr5_2:31525446-31525465 Msa0767750:intron 25.0%
! AACCACACATAAAAAATGAT+AGG - chr5_2:31525676-31525695 None:intergenic 25.0%
TTCTTTCTTAGCATCTTTCT+TGG - chr5_2:31523026-31523045 None:intergenic 30.0%
GAATGTGTTGAATAGGTTAA+TGG + chr5_2:31523165-31523184 Msa0767750:five_prime_UTR 30.0%
!!! TGGTGTTTTGATGATTGTAA+TGG + chr5_2:31523519-31523538 Msa0767750:intron 30.0%
TGTTATAAGACCACAATCAT+TGG + chr5_2:31523566-31523585 Msa0767750:intron 30.0%
!! ATATTTTGAGCCAATGATTG+TGG - chr5_2:31523579-31523598 None:intergenic 30.0%
TTGGCTCAAAATATGCAATT+CGG + chr5_2:31523585-31523604 Msa0767750:intron 30.0%
! CAAAATATGCAATTCGGTTT+TGG + chr5_2:31523591-31523610 Msa0767750:intron 30.0%
TTAGAGAAACTGTATAGAAG+TGG - chr5_2:31523925-31523944 None:intergenic 30.0%
ATCATAAGTTGTTCAGTTGT+AGG + chr5_2:31523946-31523965 Msa0767750:intron 30.0%
TCATAAGTTGTTCAGTTGTA+GGG + chr5_2:31523947-31523966 Msa0767750:intron 30.0%
GTGAATGGGATGTATAAATA+GGG + chr5_2:31524048-31524067 Msa0767750:intron 30.0%
TGAATGGGATGTATAAATAG+GGG + chr5_2:31524049-31524068 Msa0767750:intron 30.0%
TAAAGGAAAGAGAGATTACA+AGG + chr5_2:31524114-31524133 Msa0767750:intron 30.0%
AAAGGAAAGAGAGATTACAA+GGG + chr5_2:31524115-31524134 Msa0767750:intron 30.0%
! TTTGTTCATGTTCCTTTTAG+AGG + chr5_2:31524185-31524204 Msa0767750:intron 30.0%
! GTTTCTTTTCAATCTGTTTC+TGG + chr5_2:31524523-31524542 Msa0767750:intron 30.0%
! TATTTGGTTCCTTGCATATT+AGG - chr5_2:31524611-31524630 None:intergenic 30.0%
CTTAAGAACGAAGATGAAAA+AGG - chr5_2:31524688-31524707 None:intergenic 30.0%
GTATCTTCCTTCTTAATAAC+AGG + chr5_2:31524767-31524786 Msa0767750:intron 30.0%
!!! GGTATACCAAAGAGTTTTTT+TGG + chr5_2:31525077-31525096 Msa0767750:intron 30.0%
!!! TTTTGGTGCTTGGTAATTTT+TGG + chr5_2:31525264-31525283 Msa0767750:intron 30.0%
! CTGTGGTACAACTTAAATTT+TGG + chr5_2:31525393-31525412 Msa0767750:intron 30.0%
!! GTACAACTTAAATTTTGGGT+TGG + chr5_2:31525398-31525417 Msa0767750:intron 30.0%
!!! ACAACTTAAATTTTGGGTTG+GGG + chr5_2:31525400-31525419 Msa0767750:intron 30.0%
!! AAAACTTTGGCAGTTTTATC+AGG - chr5_2:31525529-31525548 None:intergenic 30.0%
!!! AAACTGCCAAAGTTTTGAAA+TGG + chr5_2:31525533-31525552 Msa0767750:intron 30.0%
CATTCACCATTTCAAAACTT+TGG - chr5_2:31525542-31525561 None:intergenic 30.0%
!!! CAAAGTTTTGAAATGGTGAA+TGG + chr5_2:31525540-31525559 Msa0767750:intron 30.0%
!!! AGCCTATCATTTTTTATGTG+TGG + chr5_2:31525671-31525690 Msa0767750:CDS 30.0%
!!! TCATTTTTTATGTGTGGTTC+AGG + chr5_2:31525677-31525696 Msa0767750:CDS 30.0%
! ATGAATACTGCAAGTTTTCA+AGG - chr5_2:31525719-31525738 None:intergenic 30.0%
!!! TCGATATTTTTTGGCTGACA+CGG - chr5_2:31522911-31522930 None:intergenic 35.0%
!!! GGAGTTGGTTCGATATTTTT+TGG - chr5_2:31522920-31522939 None:intergenic 35.0%
TTGCTTCATTGAGTTAGAAG+GGG - chr5_2:31522942-31522961 None:intergenic 35.0%
CTTGCTTCATTGAGTTAGAA+GGG - chr5_2:31522943-31522962 None:intergenic 35.0%
GAAAGAAAAATCACCCAACA+AGG + chr5_2:31523039-31523058 Msa0767750:five_prime_UTR 35.0%
! TTAGCAGCATTTTCCTTGTT+GGG - chr5_2:31523055-31523074 None:intergenic 35.0%
TTCACAGGAAGAATTTGTGA+AGG + chr5_2:31523102-31523121 Msa0767750:five_prime_UTR 35.0%
TCACAGGAAGAATTTGTGAA+GGG + chr5_2:31523103-31523122 Msa0767750:five_prime_UTR 35.0%
GGGGAATGTAGTTATCAAAT+GGG + chr5_2:31523124-31523143 Msa0767750:five_prime_UTR 35.0%
TAGGTTAATGGAATCGATTG+AGG + chr5_2:31523177-31523196 Msa0767750:five_prime_UTR 35.0%
AGGTTAATGGAATCGATTGA+GGG + chr5_2:31523178-31523197 Msa0767750:five_prime_UTR 35.0%
AGAAGGAAGAATTGGAAAGA+AGG + chr5_2:31523206-31523225 Msa0767750:five_prime_UTR 35.0%
TCAAGAGTTGAAACTGTTTG+TGG + chr5_2:31523246-31523265 Msa0767750:five_prime_UTR 35.0%
! AGAGACAAATCGCTTGATTT+TGG + chr5_2:31523288-31523307 Msa0767750:five_prime_UTR 35.0%
!!! GGTTTGGGAAGTTTTTTGTA+TGG + chr5_2:31523347-31523366 Msa0767750:intron 35.0%
CAAATGCAATGCATAGATTG+TGG + chr5_2:31523495-31523514 Msa0767750:intron 35.0%
!! ATTGTGATGCTTGTTGTGTT+AGG + chr5_2:31523645-31523664 Msa0767750:intron 35.0%
!! TTGTGATGCTTGTTGTGTTA+GGG + chr5_2:31523646-31523665 Msa0767750:intron 35.0%
! CTTGTTGTGTTAGGGATATT+GGG + chr5_2:31523654-31523673 Msa0767750:intron 35.0%
! GATATGGTTCGTTTGTTAGT+TGG + chr5_2:31523699-31523718 Msa0767750:intron 35.0%
CAAGTGACATTATCCCTAAA+AGG - chr5_2:31523784-31523803 None:intergenic 35.0%
TGCTGAAATTGAATAGCACT+AGG + chr5_2:31523889-31523908 Msa0767750:intron 35.0%
AAATCAATAATGTCTCCACC+TGG - chr5_2:31523988-31524007 None:intergenic 35.0%
! TTTTTGTGACAAGAAGAGAG+AGG + chr5_2:31524026-31524045 Msa0767750:intron 35.0%
GGTGAATGGGATGTATAAAT+AGG + chr5_2:31524047-31524066 Msa0767750:intron 35.0%
GAATGGGATGTATAAATAGG+GGG + chr5_2:31524050-31524069 Msa0767750:intron 35.0%
AAAGAGAGATTACAAGGGTT+AGG + chr5_2:31524120-31524139 Msa0767750:intron 35.0%
AAGAGAGATTACAAGGGTTA+GGG + chr5_2:31524121-31524140 Msa0767750:intron 35.0%
GGTAGTATGGTGAATAAGAT+GGG + chr5_2:31524142-31524161 Msa0767750:intron 35.0%
TACTTGAATGCTCCTCTAAA+AGG - chr5_2:31524200-31524219 None:intergenic 35.0%
!! TTTAGAGGAGCATTCAAGTA+TGG + chr5_2:31524200-31524219 Msa0767750:intron 35.0%
TTGCGTAAAAACTATGTTGG+AGG - chr5_2:31524452-31524471 None:intergenic 35.0%
TTTGTGTTGCCTAATATGCA+AGG + chr5_2:31524599-31524618 Msa0767750:intron 35.0%
! GAAAAAGGCACATTCATTTG+TGG - chr5_2:31524673-31524692 None:intergenic 35.0%
GTCATACAAAAGGTCTCATT+TGG - chr5_2:31525011-31525030 None:intergenic 35.0%
! ATGAGACCTTTTGTATGACA+CGG + chr5_2:31525012-31525031 Msa0767750:intron 35.0%
TTAAGACCGTGTCATACAAA+AGG - chr5_2:31525021-31525040 None:intergenic 35.0%
TACAGCCCAAAAAAACTCTT+TGG - chr5_2:31525086-31525105 None:intergenic 35.0%
!!! TGTATTCTTGTTGGTTTTGG+AGG + chr5_2:31525122-31525141 Msa0767750:intron 35.0%
! TGATTTGTAGTGTGATAGCT+AGG + chr5_2:31525196-31525215 Msa0767750:intron 35.0%
!!! TTTTGGAAGATTTTGGCTTC+AGG + chr5_2:31525281-31525300 Msa0767750:intron 35.0%
!!! TTTGGAAGATTTTGGCTTCA+GGG + chr5_2:31525282-31525301 Msa0767750:intron 35.0%
! TTAAGTTGTACCACAGTTAC+AGG - chr5_2:31525389-31525408 None:intergenic 35.0%
AGAAATACAACCAACTGCAA+GGG - chr5_2:31525503-31525522 None:intergenic 35.0%
AAGAAATACAACCAACTGCA+AGG - chr5_2:31525504-31525523 None:intergenic 35.0%
GGCAAGAAACAATTTCTTCA+TGG - chr5_2:31525617-31525636 None:intergenic 35.0%
! CAAGAATGGAAGTATGTTCA+AGG - chr5_2:31525641-31525660 None:intergenic 35.0%
TGAACATACTTCCATTCTTG+TGG + chr5_2:31525641-31525660 Msa0767750:CDS 35.0%
!! TACTGCAAGTTTTCAAGGAA+GGG - chr5_2:31525714-31525733 None:intergenic 35.0%
!! ATACTGCAAGTTTTCAAGGA+AGG - chr5_2:31525715-31525734 None:intergenic 35.0%
TGCTTCATTGAGTTAGAAGG+GGG - chr5_2:31522941-31522960 None:intergenic 40.0%
GCTTGCTTCATTGAGTTAGA+AGG - chr5_2:31522944-31522963 None:intergenic 40.0%
! CTTAGCAGCATTTTCCTTGT+TGG - chr5_2:31523056-31523075 None:intergenic 40.0%
AAAATGCTGCTAAGAAACCG+AGG + chr5_2:31523062-31523081 Msa0767750:five_prime_UTR 40.0%
TGTGAATACAGTGTCTCTTC+TGG - chr5_2:31523088-31523107 None:intergenic 40.0%
AGAAGAGACACTGTATTCAC+AGG + chr5_2:31523087-31523106 Msa0767750:five_prime_UTR 40.0%
CACAGGAAGAATTTGTGAAG+GGG + chr5_2:31523104-31523123 Msa0767750:five_prime_UTR 40.0%
ACAGGAAGAATTTGTGAAGG+GGG + chr5_2:31523105-31523124 Msa0767750:five_prime_UTR 40.0%
GGGGGAATGTAGTTATCAAA+TGG + chr5_2:31523123-31523142 Msa0767750:five_prime_UTR 40.0%
AATGGAATCGATTGAGGGAT+CGG + chr5_2:31523183-31523202 Msa0767750:five_prime_UTR 40.0%
AGGAAGAATTGGAAAGAAGG+TGG + chr5_2:31523209-31523228 Msa0767750:five_prime_UTR 40.0%
AGAGTTGAAACTGTTTGTGG+AGG + chr5_2:31523249-31523268 Msa0767750:five_prime_UTR 40.0%
! GACAAATCGCTTGATTTTGG+AGG + chr5_2:31523291-31523310 Msa0767750:five_prime_UTR 40.0%
TGCAATGCATAGATTGTGGA+TGG + chr5_2:31523499-31523518 Msa0767750:intron 40.0%
!! GCTTGTTGTGTTAGGGATAT+TGG + chr5_2:31523653-31523672 Msa0767750:intron 40.0%
ACAAGAAGAGAGAGGTGAAT+GGG + chr5_2:31524034-31524053 Msa0767750:intron 40.0%
TTACAAGGGTTAGGGTAGTA+TGG + chr5_2:31524129-31524148 Msa0767750:intron 40.0%
GGGTAGTATGGTGAATAAGA+TGG + chr5_2:31524141-31524160 Msa0767750:intron 40.0%
CGTAAAAACTATGTTGGAGG+AGG - chr5_2:31524449-31524468 None:intergenic 40.0%
CTGTCACAGATAAACCGTAA+AGG - chr5_2:31524728-31524747 None:intergenic 40.0%
CTTTACGGTTTATCTGTGAC+AGG + chr5_2:31524726-31524745 Msa0767750:intron 40.0%
! TCTGTGACAGGTTCTGTTTT+AGG + chr5_2:31524738-31524757 Msa0767750:intron 40.0%
! CTGTGACAGGTTCTGTTTTA+GGG + chr5_2:31524739-31524758 Msa0767750:intron 40.0%
GTATGCTAAGTTTACTGGAC+TGG + chr5_2:31524847-31524866 Msa0767750:intron 40.0%
!!! TTGGAAGATTTTGGCTTCAG+GGG + chr5_2:31525283-31525302 Msa0767750:intron 40.0%
!!! ATTTTGGCTTCAGGGGATAT+TGG + chr5_2:31525290-31525309 Msa0767750:intron 40.0%
TAAGCTGTAACCTGTAACTG+TGG + chr5_2:31525376-31525395 Msa0767750:intron 40.0%
AAACCAAAAACCCTTGCAGT+TGG + chr5_2:31525490-31525509 Msa0767750:intron 40.0%
! GAATGGAAGTATGTTCAAGG+AGG - chr5_2:31525638-31525657 None:intergenic 40.0%
! GGCTTTATAGTCCACAAGAA+TGG - chr5_2:31525655-31525674 None:intergenic 40.0%
ATTGAGTTAGAAGGGGGAGT+TGG - chr5_2:31522935-31522954 None:intergenic 45.0%
!! ATCGCTTGATTTTGGAGGCA+TGG + chr5_2:31523296-31523315 Msa0767750:five_prime_UTR 45.0%
TAGACACGAAGCATGAGGTT+TGG + chr5_2:31523331-31523350 Msa0767750:intron 45.0%
AGACACGAAGCATGAGGTTT+GGG + chr5_2:31523332-31523351 Msa0767750:intron 45.0%
!! CTCCATTTTGATGAACCAGG+TGG + chr5_2:31523970-31523989 Msa0767750:intron 45.0%
CTCCACCTGGTTCATCAAAA+TGG - chr5_2:31523975-31523994 None:intergenic 45.0%
GACAAGAAGAGAGAGGTGAA+TGG + chr5_2:31524033-31524052 Msa0767750:intron 45.0%
! AGGAGGATCTTTTCGCAATG+GGG - chr5_2:31524432-31524451 None:intergenic 45.0%
! GAGGAGGATCTTTTCGCAAT+GGG - chr5_2:31524433-31524452 None:intergenic 45.0%
! TGCTAAGTTTACTGGACTGG+TGG + chr5_2:31524850-31524869 Msa0767750:intron 45.0%
!! TTACTGGACTGGTGGTCTTA+TGG + chr5_2:31524858-31524877 Msa0767750:intron 45.0%
AATGATCTGTGCAATCGTGC+AGG + chr5_2:31525229-31525248 Msa0767750:intron 45.0%
CTTCAGGGGATATTGGCATT+AGG + chr5_2:31525297-31525316 Msa0767750:intron 45.0%
GGGATATTGGCATTAGGTGT+CGG + chr5_2:31525303-31525322 Msa0767750:intron 45.0%
GGATATTGGCATTAGGTGTC+GGG + chr5_2:31525304-31525323 Msa0767750:intron 45.0%
! CAACCAACTGCAAGGGTTTT+TGG - chr5_2:31525496-31525515 None:intergenic 45.0%
! GGCTGACACGGATTGAGATT+CGG - chr5_2:31522899-31522918 None:intergenic 50.0%
! TTGGTAAAGCGCCTCTTACG+CGG - chr5_2:31523007-31523026 None:intergenic 50.0%
TACAGTGTCTCTTCTGGCCT+CGG - chr5_2:31523082-31523101 None:intergenic 50.0%
ATCGATTGAGGGATCGGAGA+AGG + chr5_2:31523189-31523208 Msa0767750:five_prime_UTR 50.0%
GGGATCGGAGAAGGAAGAAT+TGG + chr5_2:31523198-31523217 Msa0767750:five_prime_UTR 50.0%
!! TTGATTTTGGAGGCATGGCG+AGG + chr5_2:31523301-31523320 Msa0767750:five_prime_UTR 50.0%
CGTCTTAGACACGAAGCATG+AGG + chr5_2:31523326-31523345 Msa0767750:exon 50.0%
!! GGGCTCCATTTTGATGAACC+AGG + chr5_2:31523967-31523986 Msa0767750:intron 50.0%
! GGAGGAGGATCTTTTCGCAA+TGG - chr5_2:31524434-31524453 None:intergenic 50.0%
!!! GCAGGTTCTGTGCTGGTTTT+TGG + chr5_2:31525247-31525266 Msa0767750:intron 50.0%
!!! CTGTGCTGGTTTTTGGTGCT+TGG + chr5_2:31525254-31525273 Msa0767750:intron 50.0%
GTTTGATGAGTCTGCAGCGG+CGG - chr5_2:31522981-31523000 None:intergenic 55.0%
! CCGGTTTGATGAGTCTGCAG+CGG - chr5_2:31522984-31523003 None:intergenic 55.0%
CCGCTGCAGACTCATCAAAC+CGG + chr5_2:31522981-31523000 Msa0767750:five_prime_UTR 55.0%
CAATCGTGCAGGTTCTGTGC+TGG + chr5_2:31525240-31525259 Msa0767750:intron 55.0%
CATCAAACCGGCCGCGTAAG+AGG + chr5_2:31522993-31523012 Msa0767750:five_prime_UTR 60.0%
! TAAAGCGCCTCTTACGCGGC+CGG - chr5_2:31523003-31523022 None:intergenic 60.0%
TGATGAGTCTGCAGCGGCGG+CGG - chr5_2:31522978-31522997 None:intergenic 65.0%
TGAGTCTGCAGCGGCGGCGG+CGG - chr5_2:31522975-31522994 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 31522890 31525743 31522890 ID=Msa0767750;Name=Msa0767750
chr5_2 mRNA 31522890 31525743 31522890 ID=Msa0767750-mRNA-1;Parent=Msa0767750;Name=Msa0767750-mRNA-1;_AED=0.28;_eAED=0.43;_QI=453|0|0.25|0.75|1|1|4|0|87
chr5_2 exon 31522890 31523347 31522890 ID=Msa0767750-mRNA-1:exon:11610;Parent=Msa0767750-mRNA-1
chr5_2 exon 31524367 31524459 31524367 ID=Msa0767750-mRNA-1:exon:11611;Parent=Msa0767750-mRNA-1
chr5_2 exon 31525369 31525374 31525369 ID=Msa0767750-mRNA-1:exon:11612;Parent=Msa0767750-mRNA-1
chr5_2 exon 31525584 31525743 31525584 ID=Msa0767750-mRNA-1:exon:11613;Parent=Msa0767750-mRNA-1
chr5_2 five_prime_UTR 31522890 31523342 31522890 ID=Msa0767750-mRNA-1:five_prime_utr;Parent=Msa0767750-mRNA-1
chr5_2 CDS 31523343 31523347 31523343 ID=Msa0767750-mRNA-1:cds;Parent=Msa0767750-mRNA-1
chr5_2 CDS 31524367 31524459 31524367 ID=Msa0767750-mRNA-1:cds;Parent=Msa0767750-mRNA-1
chr5_2 CDS 31525369 31525374 31525369 ID=Msa0767750-mRNA-1:cds;Parent=Msa0767750-mRNA-1
chr5_2 CDS 31525584 31525743 31525584 ID=Msa0767750-mRNA-1:cds;Parent=Msa0767750-mRNA-1
Gene Sequence

>Msa0767750

ATGAGTTTGCTTGCTTTCACAATAAAACCAATTGACAAAACATTTCACTTGTCTACACAAAGTGATTCCCCATTGCGAAAAGATCCTCCTCCAACATATAAGTTATTTCGAGATGCTGCTTTATTTTCTTCTAACCATGAAGAAATTGTTTCTTGCCTCCTTGAACATACTTCCATTCTTGTGGACTATAAAGCCTATCATTTTTTATGTGTGGTTCAGGAACAGTTTGAGCCCTTCCTTGAAAACTTGCAGTATTCATTGTGA
Protein sequence

>Msa0767750

MSLLAFTIKPIDKTFHLSTQSDSPLRKDPPPTYKLFRDAALFSSNHEEIVSCLLEHTSILVDYKAYHFLCVVQEQFEPFLENLQYSL*