Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0783490 | sp|P38385|SC61G_ORYSJ | 94.203 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 2.43e-41 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0783490 | A0A4D6MJ31 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 5.14e-41 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0633570 | Msa0783490 | 0.812203 | 4.771050e-51 | -8.615850e-47 |
| Msa0705000 | Msa0783490 | 0.810865 | 9.317685e-51 | -8.615850e-47 |
| Msa0705040 | Msa0783490 | 0.807387 | 5.182179e-50 | -8.615850e-47 |
| Msa0706500 | Msa0783490 | 0.815910 | 7.257617e-52 | -8.615850e-47 |
| Msa0744090 | Msa0783490 | 0.804064 | 2.584891e-49 | -8.615850e-47 |
| Msa0783490 | Msa0819540 | 0.854559 | 1.135186e-61 | -8.615850e-47 |
| Msa0783490 | Msa0825280 | 0.801582 | 8.410687e-49 | -8.615850e-47 |
| Msa0783490 | Msa0851470 | 0.805500 | 1.295761e-49 | -8.615850e-47 |
| Msa0783490 | Msa0963020 | 0.826137 | 3.200136e-54 | -8.615850e-47 |
| Msa0783490 | Msa0968440 | 0.812982 | 3.222689e-51 | -8.615850e-47 |
| Msa0783490 | Msa1205850 | 0.808438 | 3.096419e-50 | -8.615850e-47 |
| Msa0783490 | Msa1207730 | 0.801603 | 8.329966e-49 | -8.615850e-47 |
| Msa0783490 | Msa1435440 | 0.816942 | 4.262648e-52 | -8.615850e-47 |
| Msa0166750 | Msa0783490 | 0.802083 | 6.638271e-49 | -8.615850e-47 |
| Msa0245060 | Msa0783490 | 0.830538 | 2.776199e-55 | -8.615850e-47 |
| Msa0291520 | Msa0783490 | 0.800612 | 1.328421e-48 | -8.615850e-47 |
| Msa0512850 | Msa0783490 | 0.802203 | 6.272164e-49 | -8.615850e-47 |
| Msa0549790 | Msa0783490 | 0.800090 | 1.696725e-48 | -8.615850e-47 |
| Msa0593700 | Msa0783490 | 0.809927 | 1.485161e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0783490 | MtrunA17_Chr5g0438181 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.09e-44 | 139 |
| Msa0783490 | MtrunA17_Chr4g0071611 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.72e-44 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0783490 | AT4G24920.1 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 4.96e-41 | 128 |
| Msa0783490 | AT5G50460.1 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 4.96e-41 | 128 |
| Msa0783490 | AT3G48570.1 | 86.957 | 69 | 9 | 0 | 1 | 69 | 1 | 69 | 2.17e-39 | 124 |
Find 18 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGATTGGTTTCGTTGTGA+TGG | 0.380019 | 5_2:+67546466 | Msa0783490:CDS |
| TTCGTTGTGATGGGATTTGT+TGG | 0.388700 | 5_2:+67546476 | Msa0783490:CDS |
| TACCTTTGCGATCGGGTTTG+TGG | 0.417825 | 5_2:-67546327 | None:intergenic |
| CGGGTTATACCTTTGCGATC+GGG | 0.418283 | 5_2:-67546334 | None:intergenic |
| CCAACGATGATGTTGTTGAT+TGG | 0.436566 | 5_2:-67546524 | None:intergenic |
| CGCGGTACGAACAGCAACCT+TGG | 0.443094 | 5_2:-67546447 | None:intergenic |
| CATCATCGTTGGATCTGGTT+AGG | 0.451612 | 5_2:+67546535 | Msa0783490:CDS |
| AACAACATCATCGTTGGATC+TGG | 0.457089 | 5_2:+67546530 | Msa0783490:CDS |
| TGGATCGAAGACGGAATCAA+TGG | 0.467824 | 5_2:-67546260 | None:intergenic |
| GTTGCTGTTCGTACCGCGAT+TGG | 0.481738 | 5_2:+67546452 | Msa0783490:CDS |
| TCGTTGTGATGGGATTTGTT+GGG | 0.487876 | 5_2:+67546477 | Msa0783490:CDS |
| GCGATTGGTTTCGTTGTGAT+GGG | 0.488493 | 5_2:+67546467 | Msa0783490:CDS |
| AATTCGCAAAAGACAGCGTT+AGG | 0.495326 | 5_2:+67546290 | Msa0783490:CDS |
| ACGGGTTATACCTTTGCGAT+CGG | 0.500665 | 5_2:-67546335 | None:intergenic |
| TTCTCTGAGTGGATCGAAGA+CGG | 0.515282 | 5_2:-67546269 | None:intergenic |
| CCAATCAACAACATCATCGT+TGG | 0.538152 | 5_2:+67546524 | Msa0783490:CDS |
| TGCCACAAACCCGATCGCAA+AGG | 0.648616 | 5_2:+67546325 | Msa0783490:CDS |
| CATCACAACGAAACCAATCG+CGG | 0.678324 | 5_2:-67546465 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGATTGTTTTTTTTTTT+TGG | + | chr5_2:67546409-67546428 | Msa0783490:intron | 10.0% |
| !! | TGAAGAGAAAATTGATTTAC+GGG | - | chr5_2:67546356-67546375 | None:intergenic | 25.0% |
| !! | GTGAAGAGAAAATTGATTTA+CGG | - | chr5_2:67546357-67546376 | None:intergenic | 25.0% |
| ! | TGATTTCAATCTATTTATCG+TGG | + | chr5_2:67546378-67546397 | Msa0783490:intron | 25.0% |
| ! | GGCTTTTGTAGAATTTTCCA+AGG | + | chr5_2:67546430-67546449 | Msa0783490:intron | 35.0% |
| ! | TCTTTTGCGAATTCTCTGAG+TGG | - | chr5_2:67546283-67546302 | None:intergenic | 40.0% |
| AATTCGCAAAAGACAGCGTT+AGG | + | chr5_2:67546290-67546309 | Msa0783490:CDS | 40.0% | |
| ! | TTCGTTGTGATGGGATTTGT+TGG | + | chr5_2:67546476-67546495 | Msa0783490:CDS | 40.0% |
| ! | TCGTTGTGATGGGATTTGTT+GGG | + | chr5_2:67546477-67546496 | Msa0783490:CDS | 40.0% |
| CCAACGATGATGTTGTTGAT+TGG | - | chr5_2:67546527-67546546 | None:intergenic | 40.0% | |
| CCAATCAACAACATCATCGT+TGG | + | chr5_2:67546524-67546543 | Msa0783490:CDS | 40.0% | |
| AACAACATCATCGTTGGATC+TGG | + | chr5_2:67546530-67546549 | Msa0783490:CDS | 40.0% | |
| TGGATCGAAGACGGAATCAA+TGG | - | chr5_2:67546263-67546282 | None:intergenic | 45.0% | |
| TTCTCTGAGTGGATCGAAGA+CGG | - | chr5_2:67546272-67546291 | None:intergenic | 45.0% | |
| ! | ACGGGTTATACCTTTGCGAT+CGG | - | chr5_2:67546338-67546357 | None:intergenic | 45.0% |
| CATCACAACGAAACCAATCG+CGG | - | chr5_2:67546468-67546487 | None:intergenic | 45.0% | |
| !! | GCGATTGGTTTCGTTGTGAT+GGG | + | chr5_2:67546467-67546486 | Msa0783490:CDS | 45.0% |
| TACCTTTGCGATCGGGTTTG+TGG | - | chr5_2:67546330-67546349 | None:intergenic | 50.0% | |
| CGGGTTATACCTTTGCGATC+GGG | - | chr5_2:67546337-67546356 | None:intergenic | 50.0% | |
| !! | CGCGATTGGTTTCGTTGTGA+TGG | + | chr5_2:67546466-67546485 | Msa0783490:CDS | 50.0% |
| TGCCACAAACCCGATCGCAA+AGG | + | chr5_2:67546325-67546344 | Msa0783490:CDS | 55.0% | |
| ! | GTTGCTGTTCGTACCGCGAT+TGG | + | chr5_2:67546452-67546471 | Msa0783490:CDS | 55.0% |
| CGCGGTACGAACAGCAACCT+TGG | - | chr5_2:67546450-67546469 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 67546253 | 67546556 | 67546253 | ID=Msa0783490;Name=Msa0783490 |
| chr5_2 | mRNA | 67546253 | 67546556 | 67546253 | ID=Msa0783490-mRNA-1;Parent=Msa0783490;Name=Msa0783490-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|69 |
| chr5_2 | exon | 67546253 | 67546346 | 67546253 | ID=Msa0783490-mRNA-1:exon:20223;Parent=Msa0783490-mRNA-1 |
| chr5_2 | exon | 67546441 | 67546556 | 67546441 | ID=Msa0783490-mRNA-1:exon:20224;Parent=Msa0783490-mRNA-1 |
| chr5_2 | CDS | 67546253 | 67546346 | 67546253 | ID=Msa0783490-mRNA-1:cds;Parent=Msa0783490-mRNA-1 |
| chr5_2 | CDS | 67546441 | 67546556 | 67546441 | ID=Msa0783490-mRNA-1:cds;Parent=Msa0783490-mRNA-1 |
| Gene Sequence |
| Protein sequence |
