AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0797880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797880 MtrunA17_Chr5g0401391 97.810 137 2 1 1 136 1 137 5.04e-97 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797880 AT1G07830.1 71.528 144 33 3 1 136 1 144 4.58e-67 199

Find 51 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATCATTTGGAGCTTAAA+TGG 0.098768 5_3:-10218441 Msa0797880:three_prime_UTR
ATGGTCTTTGATGACTTAAA+AGG 0.289344 5_3:-10218752 Msa0797880:exon
CTGAATTGCGTCTGAAATCT+TGG 0.295639 5_3:-10219228 Msa0797880:CDS
GGGGTTACTCACTCATCATT+TGG 0.370278 5_3:-10218452 Msa0797880:three_prime_UTR
TGAGACTGTTAAAGAAATAA+AGG 0.381017 5_3:-10218508 Msa0797880:three_prime_UTR
TTTGGGTATGCTGTAAGAAT+AGG 0.395902 5_3:-10218711 Msa0797880:three_prime_UTR
CCCTCTTCCGGAGTTCTTTG+AGG 0.397007 5_3:-10219443 Msa0797880:CDS
GAGATGAAGAAAATGATAAA+TGG 0.401349 5_3:-10218771 Msa0797880:CDS
TTAGGTAAGCGCTCCGGGTT+AGG 0.401390 5_3:+10219112 None:intergenic
GAATAGGACATCTACTTTCA+TGG 0.415235 5_3:-10218695 Msa0797880:three_prime_UTR
GTTGTGGTATGTGTTGTTAA+AGG 0.420053 5_3:-10219191 Msa0797880:CDS
AACGAGACACCAGTTGTCTA+TGG 0.421926 5_3:-10219409 Msa0797880:intron
GATAATAAGCTCACATCTCA+TGG 0.425888 5_3:-10218474 Msa0797880:three_prime_UTR
TTCACCCACAATTATCAGCT+TGG 0.435200 5_3:+10218411 None:intergenic
TGAATTGCGTCTGAAATCTT+GGG 0.436291 5_3:-10219227 Msa0797880:CDS
TGCTGTTAGAAACCCTCTTC+CGG 0.440920 5_3:-10219455 Msa0797880:CDS
ACAATGGTTAAGTGACTCTC+AGG 0.460628 5_3:+10218580 None:intergenic
TCAGCAGACCTCCTTGCGTC+TGG 0.463615 5_3:+10218792 None:intergenic
TCATCATTTGGAGCTTAAAT+GGG 0.468977 5_3:-10218440 Msa0797880:three_prime_UTR
ATACCTTAGGTAAGCGCTCC+GGG 0.470522 5_3:+10219107 None:intergenic
AAACTCCACAATTATAAGCT+TGG 0.478967 5_3:+10218383 None:intergenic
GAACTTGCGCTTTCCTAACC+CGG 0.481233 5_3:-10219125 Msa0797880:CDS
GCCTCAAAGAACTCCGGAAG+AGG 0.493349 5_3:+10219442 None:intergenic
AAAATCCAAGCTGATAATTG+TGG 0.494181 5_3:-10218416 Msa0797880:three_prime_UTR
TATTTCATACCATAGACAAC+TGG 0.503485 5_3:+10219400 None:intergenic
CTCGTGAACATTCTGCAGAT+GGG 0.512970 5_3:+10218545 None:intergenic
AATACCTTAGGTAAGCGCTC+CGG 0.514447 5_3:+10219106 None:intergenic
AATCATGTATTTGAACAGAC+TGG 0.525051 5_3:+10218649 None:intergenic
AAATCCAAGCTGATAATTGT+GGG 0.535749 5_3:-10218415 Msa0797880:three_prime_UTR
GCTCGTGAACATTCTGCAGA+TGG 0.538875 5_3:+10218544 None:intergenic
AAACAGTATTCCACTACAGC+TGG 0.543067 5_3:-10219478 Msa0797880:CDS
TATTTCTTTAACAGTCTCAC+AGG 0.543526 5_3:+10218512 None:intergenic
ACACATACCACAACTTATGA+AGG 0.545630 5_3:+10219200 None:intergenic
TAACACCAAGCTTATAATTG+TGG 0.546576 5_3:-10218388 Msa0797880:three_prime_UTR
TTTGTTAGGTCGGAGTTGGA+AGG 0.551769 5_3:-10219254 Msa0797880:intron
TGTATTTGAACAGACTGGCT+TGG 0.552486 5_3:+10218654 None:intergenic
ATAATAAGCTCACATCTCAT+GGG 0.553972 5_3:-10218473 Msa0797880:three_prime_UTR
GGGATGACCTTCATAAGTTG+TGG 0.556409 5_3:-10219207 Msa0797880:CDS
TCTAACAGCACCAGCTGTAG+TGG 0.557909 5_3:+10219468 None:intergenic
TAACCCGGAGCGCTTACCTA+AGG 0.570629 5_3:-10219110 Msa0797880:intron
CTGTCTGCCTCAAAGAACTC+CGG 0.578032 5_3:+10219436 None:intergenic
TGGGGTTCATTTGACGACAA+TGG 0.593531 5_3:+10218564 None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG 0.599992 5_3:+10219443 None:intergenic
CGATTGAAGAACCAGACGCA+AGG 0.611981 5_3:-10218803 Msa0797880:CDS
TAATAAGCTCACATCTCATG+GGG 0.625202 5_3:-10218472 Msa0797880:three_prime_UTR
GACTGTTAAAGAAATAAAGG+TGG 0.634963 5_3:-10218505 Msa0797880:three_prime_UTR
TCGTGAACATTCTGCAGATG+GGG 0.641508 5_3:+10218546 None:intergenic
CAGTAAGCACATGCTTGATG+CGG 0.642610 5_3:+10218832 None:intergenic
TTGAAGAACCAGACGCAAGG+AGG 0.691763 5_3:-10218800 Msa0797880:CDS
CAATGGTTAAGTGACTCTCA+GGG 0.702841 5_3:+10218581 None:intergenic
AATAAGCTCACATCTCATGG+GGG 0.768256 5_3:-10218471 Msa0797880:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATGTTAAATTTTGTAT+GGG - chr5_3:10218970-10218989 Msa0797880:intron 10.0%
!!! TAAAATGTTAAATTTTGTAT+GGG - chr5_3:10218970-10218989 Msa0797880:intron 10.0%
!!! GTAAAATGTTAAATTTTGTA+TGG - chr5_3:10218969-10218988 Msa0797880:intron 15.0%
!!! GTAAAATGTTAAATTTTGTA+TGG - chr5_3:10218969-10218988 Msa0797880:intron 15.0%
!!! ATAGTTTTACCATTTTTGTT+AGG - chr5_3:10218635-10218654 Msa0797880:three_prime_UTR 20.0%
!!! GATTTAATTTATGTGAAATC+AGG - chr5_3:10219032-10219051 Msa0797880:intron 20.0%
!!! ATAGTTTTACCATTTTTGTT+AGG - chr5_3:10218635-10218654 Msa0797880:three_prime_UTR 20.0%
!!! GATTTAATTTATGTGAAATC+AGG - chr5_3:10219032-10219051 Msa0797880:intron 20.0%
!!! ATTTTTTTGTACCTAATGTG+TGG + chr5_3:10218586-10218605 None:intergenic 25.0%
! TATAAGAAAACAAAGTGATG+AGG + chr5_3:10218618-10218637 None:intergenic 25.0%
!!! TTTTACCATTTTTGTTAGGT+CGG - chr5_3:10218639-10218658 Msa0797880:three_prime_UTR 25.0%
! AAAAAGTGAAGAAATACCTT+AGG + chr5_3:10218812-10218831 None:intergenic 25.0%
!! ATGTTAAATTTTGTATGGGT+TGG - chr5_3:10218974-10218993 Msa0797880:intron 25.0%
!! TGTTAAATTTTGTATGGGTT+GGG - chr5_3:10218975-10218994 Msa0797880:intron 25.0%
! GAGATGAAGAAAATGATAAA+TGG - chr5_3:10219132-10219151 Msa0797880:CDS 25.0%
!! ATATGTTTTCGTTGCATTTT+TGG - chr5_3:10219174-10219193 Msa0797880:CDS 25.0%
!! TATGTTTTCGTTGCATTTTT+GGG - chr5_3:10219175-10219194 Msa0797880:CDS 25.0%
! TGAGACTGTTAAAGAAATAA+AGG - chr5_3:10219395-10219414 Msa0797880:intron 25.0%
!!! ATTTTTTTGTACCTAATGTG+TGG + chr5_3:10218586-10218605 None:intergenic 25.0%
! TATAAGAAAACAAAGTGATG+AGG + chr5_3:10218618-10218637 None:intergenic 25.0%
!!! TTTTACCATTTTTGTTAGGT+CGG - chr5_3:10218639-10218658 Msa0797880:three_prime_UTR 25.0%
! AAAAAGTGAAGAAATACCTT+AGG + chr5_3:10218812-10218831 None:intergenic 25.0%
!! ATGTTAAATTTTGTATGGGT+TGG - chr5_3:10218974-10218993 Msa0797880:intron 25.0%
!! TGTTAAATTTTGTATGGGTT+GGG - chr5_3:10218975-10218994 Msa0797880:intron 25.0%
! GAGATGAAGAAAATGATAAA+TGG - chr5_3:10219132-10219151 Msa0797880:CDS 25.0%
!! ATATGTTTTCGTTGCATTTT+TGG - chr5_3:10219174-10219193 Msa0797880:CDS 25.0%
!! TATGTTTTCGTTGCATTTTT+GGG - chr5_3:10219175-10219194 Msa0797880:CDS 25.0%
! TGAGACTGTTAAAGAAATAA+AGG - chr5_3:10219395-10219414 Msa0797880:intron 25.0%
TATTTCATACCATAGACAAC+TGG + chr5_3:10218506-10218525 None:intergenic 30.0%
CCTATTCTATTGAAAAGAAG+AGG + chr5_3:10218533-10218552 None:intergenic 30.0%
! CCTCTTCTTTTCAATAGAAT+AGG - chr5_3:10218530-10218549 Msa0797880:three_prime_UTR 30.0%
TTTCATTATAGCCACACATT+AGG - chr5_3:10218572-10218591 Msa0797880:three_prime_UTR 30.0%
!!! ATTTCTTCACTTTTTACACC+TGG - chr5_3:10218817-10218836 Msa0797880:CDS 30.0%
! TTTTGTTACCCTTAGTTATG+TGG - chr5_3:10218859-10218878 Msa0797880:intron 30.0%
ATCAATGTCCACATAACTAA+GGG + chr5_3:10218870-10218889 None:intergenic 30.0%
TATCAATGTCCACATAACTA+AGG + chr5_3:10218871-10218890 None:intergenic 30.0%
AGTTATGTGGACATTGATAT+AGG - chr5_3:10218872-10218891 Msa0797880:intron 30.0%
!! GTTAAATTTTGTATGGGTTG+GGG - chr5_3:10218976-10218995 Msa0797880:intron 30.0%
!!! TTAAATTTTGTATGGGTTGG+GGG - chr5_3:10218977-10218996 Msa0797880:intron 30.0%
! ATGGTCTTTGATGACTTAAA+AGG - chr5_3:10219151-10219170 Msa0797880:CDS 30.0%
AATCATGTATTTGAACAGAC+TGG + chr5_3:10219257-10219276 None:intergenic 30.0%
TATTTCTTTAACAGTCTCAC+AGG + chr5_3:10219394-10219413 None:intergenic 30.0%
GACTGTTAAAGAAATAAAGG+TGG - chr5_3:10219398-10219417 Msa0797880:intron 30.0%
ATAATAAGCTCACATCTCAT+GGG - chr5_3:10219430-10219449 Msa0797880:CDS 30.0%
! TCATCATTTGGAGCTTAAAT+GGG - chr5_3:10219463-10219482 Msa0797880:CDS 30.0%
AAAATCCAAGCTGATAATTG+TGG - chr5_3:10219487-10219506 Msa0797880:CDS 30.0%
AAATCCAAGCTGATAATTGT+GGG - chr5_3:10219488-10219507 Msa0797880:CDS 30.0%
TAACACCAAGCTTATAATTG+TGG - chr5_3:10219515-10219534 Msa0797880:CDS 30.0%
AAACTCCACAATTATAAGCT+TGG + chr5_3:10219523-10219542 None:intergenic 30.0%
TATTTCATACCATAGACAAC+TGG + chr5_3:10218506-10218525 None:intergenic 30.0%
CCTATTCTATTGAAAAGAAG+AGG + chr5_3:10218533-10218552 None:intergenic 30.0%
! CCTCTTCTTTTCAATAGAAT+AGG - chr5_3:10218530-10218549 Msa0797880:three_prime_UTR 30.0%
TTTCATTATAGCCACACATT+AGG - chr5_3:10218572-10218591 Msa0797880:three_prime_UTR 30.0%
!!! ATTTCTTCACTTTTTACACC+TGG - chr5_3:10218817-10218836 Msa0797880:CDS 30.0%
! TTTTGTTACCCTTAGTTATG+TGG - chr5_3:10218859-10218878 Msa0797880:intron 30.0%
ATCAATGTCCACATAACTAA+GGG + chr5_3:10218870-10218889 None:intergenic 30.0%
TATCAATGTCCACATAACTA+AGG + chr5_3:10218871-10218890 None:intergenic 30.0%
AGTTATGTGGACATTGATAT+AGG - chr5_3:10218872-10218891 Msa0797880:intron 30.0%
!! GTTAAATTTTGTATGGGTTG+GGG - chr5_3:10218976-10218995 Msa0797880:intron 30.0%
!!! TTAAATTTTGTATGGGTTGG+GGG - chr5_3:10218977-10218996 Msa0797880:intron 30.0%
! ATGGTCTTTGATGACTTAAA+AGG - chr5_3:10219151-10219170 Msa0797880:CDS 30.0%
AATCATGTATTTGAACAGAC+TGG + chr5_3:10219257-10219276 None:intergenic 30.0%
TATTTCTTTAACAGTCTCAC+AGG + chr5_3:10219394-10219413 None:intergenic 30.0%
GACTGTTAAAGAAATAAAGG+TGG - chr5_3:10219398-10219417 Msa0797880:intron 30.0%
ATAATAAGCTCACATCTCAT+GGG - chr5_3:10219430-10219449 Msa0797880:CDS 30.0%
! TCATCATTTGGAGCTTAAAT+GGG - chr5_3:10219463-10219482 Msa0797880:CDS 30.0%
AAAATCCAAGCTGATAATTG+TGG - chr5_3:10219487-10219506 Msa0797880:CDS 30.0%
AAATCCAAGCTGATAATTGT+GGG - chr5_3:10219488-10219507 Msa0797880:CDS 30.0%
TAACACCAAGCTTATAATTG+TGG - chr5_3:10219515-10219534 Msa0797880:CDS 30.0%
AAACTCCACAATTATAAGCT+TGG + chr5_3:10219523-10219542 None:intergenic 30.0%
AATGCTCTTGCCAAAAACAT+TGG + chr5_3:10218371-10218390 None:intergenic 35.0%
!!! AGTGGAATACTGTTTTGATC+TGG + chr5_3:10218420-10218439 None:intergenic 35.0%
! TGAATTGCGTCTGAAATCTT+GGG - chr5_3:10218676-10218695 Msa0797880:three_prime_UTR 35.0%
ACACATACCACAACTTATGA+AGG + chr5_3:10218706-10218725 None:intergenic 35.0%
! GTTGTGGTATGTGTTGTTAA+AGG - chr5_3:10218712-10218731 Msa0797880:three_prime_UTR 35.0%
ATTGATATAGGAAATCGCAG+TGG - chr5_3:10218884-10218903 Msa0797880:intron 35.0%
!!! TAAATTTTGTATGGGTTGGG+GGG - chr5_3:10218978-10218997 Msa0797880:intron 35.0%
TTTGGGTATGCTGTAAGAAT+AGG - chr5_3:10219192-10219211 Msa0797880:CDS 35.0%
GAATAGGACATCTACTTTCA+TGG - chr5_3:10219208-10219227 Msa0797880:CDS 35.0%
GATAATAAGCTCACATCTCA+TGG - chr5_3:10219429-10219448 Msa0797880:CDS 35.0%
TAATAAGCTCACATCTCATG+GGG - chr5_3:10219431-10219450 Msa0797880:CDS 35.0%
! CTCATCATTTGGAGCTTAAA+TGG - chr5_3:10219462-10219481 Msa0797880:CDS 35.0%
AATGCTCTTGCCAAAAACAT+TGG + chr5_3:10218371-10218390 None:intergenic 35.0%
!!! AGTGGAATACTGTTTTGATC+TGG + chr5_3:10218420-10218439 None:intergenic 35.0%
! TGAATTGCGTCTGAAATCTT+GGG - chr5_3:10218676-10218695 Msa0797880:three_prime_UTR 35.0%
ACACATACCACAACTTATGA+AGG + chr5_3:10218706-10218725 None:intergenic 35.0%
! GTTGTGGTATGTGTTGTTAA+AGG - chr5_3:10218712-10218731 Msa0797880:three_prime_UTR 35.0%
ATTGATATAGGAAATCGCAG+TGG - chr5_3:10218884-10218903 Msa0797880:intron 35.0%
!!! TAAATTTTGTATGGGTTGGG+GGG - chr5_3:10218978-10218997 Msa0797880:intron 35.0%
TTTGGGTATGCTGTAAGAAT+AGG - chr5_3:10219192-10219211 Msa0797880:CDS 35.0%
GAATAGGACATCTACTTTCA+TGG - chr5_3:10219208-10219227 Msa0797880:CDS 35.0%
GATAATAAGCTCACATCTCA+TGG - chr5_3:10219429-10219448 Msa0797880:CDS 35.0%
TAATAAGCTCACATCTCATG+GGG - chr5_3:10219431-10219450 Msa0797880:CDS 35.0%
! CTCATCATTTGGAGCTTAAA+TGG - chr5_3:10219462-10219481 Msa0797880:CDS 35.0%
AAACAGTATTCCACTACAGC+TGG - chr5_3:10218425-10218444 Msa0797880:three_prime_UTR 40.0%
CAACTCCGACCTAACAAAAA+TGG + chr5_3:10218647-10218666 None:intergenic 40.0%
! CATTTTTGTTAGGTCGGAGT+TGG - chr5_3:10218645-10218664 Msa0797880:three_prime_UTR 40.0%
! CTGAATTGCGTCTGAAATCT+TGG - chr5_3:10218675-10218694 Msa0797880:three_prime_UTR 40.0%
! GTGGTGCTTTTATCAACGTT+TGG - chr5_3:10218906-10218925 Msa0797880:intron 40.0%
! TGTATTTGAACAGACTGGCT+TGG + chr5_3:10219252-10219271 None:intergenic 40.0%
CAATGGTTAAGTGACTCTCA+GGG + chr5_3:10219325-10219344 None:intergenic 40.0%
ACAATGGTTAAGTGACTCTC+AGG + chr5_3:10219326-10219345 None:intergenic 40.0%
AATAAGCTCACATCTCATGG+GGG - chr5_3:10219432-10219451 Msa0797880:CDS 40.0%
TTCACCCACAATTATCAGCT+TGG + chr5_3:10219495-10219514 None:intergenic 40.0%
AAACAGTATTCCACTACAGC+TGG - chr5_3:10218425-10218444 Msa0797880:three_prime_UTR 40.0%
CAACTCCGACCTAACAAAAA+TGG + chr5_3:10218647-10218666 None:intergenic 40.0%
! CATTTTTGTTAGGTCGGAGT+TGG - chr5_3:10218645-10218664 Msa0797880:three_prime_UTR 40.0%
! CTGAATTGCGTCTGAAATCT+TGG - chr5_3:10218675-10218694 Msa0797880:three_prime_UTR 40.0%
! GTGGTGCTTTTATCAACGTT+TGG - chr5_3:10218906-10218925 Msa0797880:intron 40.0%
! TGTATTTGAACAGACTGGCT+TGG + chr5_3:10219252-10219271 None:intergenic 40.0%
CAATGGTTAAGTGACTCTCA+GGG + chr5_3:10219325-10219344 None:intergenic 40.0%
ACAATGGTTAAGTGACTCTC+AGG + chr5_3:10219326-10219345 None:intergenic 40.0%
AATAAGCTCACATCTCATGG+GGG - chr5_3:10219432-10219451 Msa0797880:CDS 40.0%
TTCACCCACAATTATCAGCT+TGG + chr5_3:10219495-10219514 None:intergenic 40.0%
TGCTGTTAGAAACCCTCTTC+CGG - chr5_3:10218448-10218467 Msa0797880:three_prime_UTR 45.0%
AACGAGACACCAGTTGTCTA+TGG - chr5_3:10218494-10218513 Msa0797880:three_prime_UTR 45.0%
TTTGTTAGGTCGGAGTTGGA+AGG - chr5_3:10218649-10218668 Msa0797880:three_prime_UTR 45.0%
GGGATGACCTTCATAAGTTG+TGG - chr5_3:10218696-10218715 Msa0797880:three_prime_UTR 45.0%
AATACCTTAGGTAAGCGCTC+CGG + chr5_3:10218800-10218819 None:intergenic 45.0%
GATATAGGAAATCGCAGTGG+TGG - chr5_3:10218887-10218906 Msa0797880:intron 45.0%
CAGTAAGCACATGCTTGATG+CGG + chr5_3:10219074-10219093 None:intergenic 45.0%
TGGGGTTCATTTGACGACAA+TGG + chr5_3:10219342-10219361 None:intergenic 45.0%
TCGTGAACATTCTGCAGATG+GGG + chr5_3:10219360-10219379 None:intergenic 45.0%
CTCGTGAACATTCTGCAGAT+GGG + chr5_3:10219361-10219380 None:intergenic 45.0%
GGGGTTACTCACTCATCATT+TGG - chr5_3:10219451-10219470 Msa0797880:CDS 45.0%
TGCTGTTAGAAACCCTCTTC+CGG - chr5_3:10218448-10218467 Msa0797880:three_prime_UTR 45.0%
AACGAGACACCAGTTGTCTA+TGG - chr5_3:10218494-10218513 Msa0797880:three_prime_UTR 45.0%
TTTGTTAGGTCGGAGTTGGA+AGG - chr5_3:10218649-10218668 Msa0797880:three_prime_UTR 45.0%
GGGATGACCTTCATAAGTTG+TGG - chr5_3:10218696-10218715 Msa0797880:three_prime_UTR 45.0%
AATACCTTAGGTAAGCGCTC+CGG + chr5_3:10218800-10218819 None:intergenic 45.0%
GATATAGGAAATCGCAGTGG+TGG - chr5_3:10218887-10218906 Msa0797880:intron 45.0%
CAGTAAGCACATGCTTGATG+CGG + chr5_3:10219074-10219093 None:intergenic 45.0%
TGGGGTTCATTTGACGACAA+TGG + chr5_3:10219342-10219361 None:intergenic 45.0%
TCGTGAACATTCTGCAGATG+GGG + chr5_3:10219360-10219379 None:intergenic 45.0%
CTCGTGAACATTCTGCAGAT+GGG + chr5_3:10219361-10219380 None:intergenic 45.0%
GGGGTTACTCACTCATCATT+TGG - chr5_3:10219451-10219470 Msa0797880:CDS 45.0%
TCTAACAGCACCAGCTGTAG+TGG + chr5_3:10218438-10218457 None:intergenic 50.0%
CCTCAAAGAACTCCGGAAGA+GGG + chr5_3:10218463-10218482 None:intergenic 50.0%
CTGTCTGCCTCAAAGAACTC+CGG + chr5_3:10218470-10218489 None:intergenic 50.0%
GAACTTGCGCTTTCCTAACC+CGG - chr5_3:10218778-10218797 Msa0797880:CDS 50.0%
ATACCTTAGGTAAGCGCTCC+GGG + chr5_3:10218799-10218818 None:intergenic 50.0%
CAACACGCGTAAATCAGACC+AGG + chr5_3:10218838-10218857 None:intergenic 50.0%
CGATTGAAGAACCAGACGCA+AGG - chr5_3:10219100-10219119 Msa0797880:intron 50.0%
TTGAAGAACCAGACGCAAGG+AGG - chr5_3:10219103-10219122 Msa0797880:intron 50.0%
GCTCGTGAACATTCTGCAGA+TGG + chr5_3:10219362-10219381 None:intergenic 50.0%
TCTAACAGCACCAGCTGTAG+TGG + chr5_3:10218438-10218457 None:intergenic 50.0%
CCTCAAAGAACTCCGGAAGA+GGG + chr5_3:10218463-10218482 None:intergenic 50.0%
CTGTCTGCCTCAAAGAACTC+CGG + chr5_3:10218470-10218489 None:intergenic 50.0%
GAACTTGCGCTTTCCTAACC+CGG - chr5_3:10218778-10218797 Msa0797880:CDS 50.0%
ATACCTTAGGTAAGCGCTCC+GGG + chr5_3:10218799-10218818 None:intergenic 50.0%
CAACACGCGTAAATCAGACC+AGG + chr5_3:10218838-10218857 None:intergenic 50.0%
CGATTGAAGAACCAGACGCA+AGG - chr5_3:10219100-10219119 Msa0797880:intron 50.0%
TTGAAGAACCAGACGCAAGG+AGG - chr5_3:10219103-10219122 Msa0797880:intron 50.0%
GCTCGTGAACATTCTGCAGA+TGG + chr5_3:10219362-10219381 None:intergenic 50.0%
CCCTCTTCCGGAGTTCTTTG+AGG - chr5_3:10218460-10218479 Msa0797880:three_prime_UTR 55.0%
GCCTCAAAGAACTCCGGAAG+AGG + chr5_3:10218464-10218483 None:intergenic 55.0%
TTAGGTAAGCGCTCCGGGTT+AGG + chr5_3:10218794-10218813 None:intergenic 55.0%
! TAACCCGGAGCGCTTACCTA+AGG - chr5_3:10218793-10218812 Msa0797880:CDS 55.0%
GGTTGGGGGGAAACTGTTCA+CGG - chr5_3:10218991-10219010 Msa0797880:intron 55.0%
CCCTCTTCCGGAGTTCTTTG+AGG - chr5_3:10218460-10218479 Msa0797880:three_prime_UTR 55.0%
GCCTCAAAGAACTCCGGAAG+AGG + chr5_3:10218464-10218483 None:intergenic 55.0%
TTAGGTAAGCGCTCCGGGTT+AGG + chr5_3:10218794-10218813 None:intergenic 55.0%
! TAACCCGGAGCGCTTACCTA+AGG - chr5_3:10218793-10218812 Msa0797880:CDS 55.0%
GGTTGGGGGGAAACTGTTCA+CGG - chr5_3:10218991-10219010 Msa0797880:intron 55.0%
TCAGCAGACCTCCTTGCGTC+TGG + chr5_3:10219114-10219133 None:intergenic 60.0%
TCAGCAGACCTCCTTGCGTC+TGG + chr5_3:10219114-10219133 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 10218366 10219559 10218366 ID=Msa0797880;Name=Msa0797880
chr5_3 mRNA 10218366 10219559 10218366 ID=Msa0797880-mRNA-1;Parent=Msa0797880;Name=Msa0797880-mRNA-1;_AED=0.08;_eAED=0.08;_QI=5|1|1|1|1|1|3|398|136
chr5_3 exon 10218366 10218871 10218366 ID=Msa0797880-mRNA-1:exon:4345;Parent=Msa0797880-mRNA-1
chr5_3 exon 10219111 10219268 10219111 ID=Msa0797880-mRNA-1:exon:4344;Parent=Msa0797880-mRNA-1
chr5_3 exon 10219410 10219559 10219410 ID=Msa0797880-mRNA-1:exon:4343;Parent=Msa0797880-mRNA-1
chr5_3 five_prime_UTR 10219555 10219559 10219555 ID=Msa0797880-mRNA-1:five_prime_utr;Parent=Msa0797880-mRNA-1
chr5_3 CDS 10219410 10219554 10219410 ID=Msa0797880-mRNA-1:cds;Parent=Msa0797880-mRNA-1
chr5_3 CDS 10219111 10219268 10219111 ID=Msa0797880-mRNA-1:cds;Parent=Msa0797880-mRNA-1
chr5_3 CDS 10218764 10218871 10218764 ID=Msa0797880-mRNA-1:cds;Parent=Msa0797880-mRNA-1
chr5_3 three_prime_UTR 10218366 10218763 10218366 ID=Msa0797880-mRNA-1:three_prime_utr;Parent=Msa0797880-mRNA-1
Gene Sequence

>Msa0797880

ATGTTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCAAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA
Protein sequence

>Msa0797880

MFLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL*