AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0756260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0756260 MtrunA17_Chr5g0401391 97.810 137 2 1 1 136 1 137 5.04e-97 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0756260 AT1G07830.1 71.528 144 33 3 1 136 1 144 4.58e-67 199

Find 25 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAATTGCGTCTGAAATCT+TGG 0.295639 5_2:-10332884 Msa0756260:CDS
CCCTCTTCCGGAGTTCTTTG+AGG 0.397007 5_2:-10333099 Msa0756260:CDS
GAGATGAAGAAAATGATAAA+TGG 0.401349 5_2:-10332427 Msa0756260:CDS
TTAGGTAAGCGCTCCGGGTT+AGG 0.401390 5_2:+10332768 None:intergenic
GTTGTGGTATGTGTTGTTAA+AGG 0.420053 5_2:-10332847 Msa0756260:CDS
AACGAGACACCAGTTGTCTA+TGG 0.421926 5_2:-10333065 Msa0756260:intron
TGAATTGCGTCTGAAATCTT+GGG 0.436291 5_2:-10332883 Msa0756260:CDS
TGCTGTTAGAAACCCTCTTC+CGG 0.440920 5_2:-10333111 Msa0756260:CDS
TCAGCAGACCTCCTTGCGTC+TGG 0.463615 5_2:+10332448 None:intergenic
ATACCTTAGGTAAGCGCTCC+GGG 0.470522 5_2:+10332763 None:intergenic
GAACTTGCGCTTTCCTAACC+CGG 0.481233 5_2:-10332781 Msa0756260:CDS
GCCTCAAAGAACTCCGGAAG+AGG 0.493349 5_2:+10333098 None:intergenic
TATTTCATACCATAGACAAC+TGG 0.503485 5_2:+10333056 None:intergenic
AATACCTTAGGTAAGCGCTC+CGG 0.514447 5_2:+10332762 None:intergenic
AAACAGTATTCCACTACAGC+TGG 0.543067 5_2:-10333134 Msa0756260:CDS
ACACATACCACAACTTATGA+AGG 0.545630 5_2:+10332856 None:intergenic
TTTGTTAGGTCGGAGTTGGA+AGG 0.551769 5_2:-10332910 Msa0756260:intron
GGGATGACCTTCATAAGTTG+TGG 0.556409 5_2:-10332863 Msa0756260:CDS
TCTAACAGCACCAGCTGTAG+TGG 0.557909 5_2:+10333124 None:intergenic
TAACCCGGAGCGCTTACCTA+AGG 0.570629 5_2:-10332766 Msa0756260:intron
CTGTCTGCCTCAAAGAACTC+CGG 0.578032 5_2:+10333092 None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG 0.599992 5_2:+10333099 None:intergenic
CGATTGAAGAACCAGACGCA+AGG 0.611981 5_2:-10332459 Msa0756260:CDS
CAGTAAGCACATGCTTGATG+CGG 0.642610 5_2:+10332488 None:intergenic
TTGAAGAACCAGACGCAAGG+AGG 0.691763 5_2:-10332456 Msa0756260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATGTTAAATTTCGTAT+GGG - chr5_2:10333019-10333038 Msa0756260:intron 15.0%
!! TAAAATGTTAAATTTCGTAT+GGG - chr5_2:10333019-10333038 Msa0756260:intron 15.0%
!!! ATAGTTTTACCATTTTTGTT+AGG - chr5_2:10332684-10332703 Msa0756260:intron 20.0%
!! GTAAAATGTTAAATTTCGTA+TGG - chr5_2:10333018-10333037 Msa0756260:intron 20.0%
!!! GATTTAATTTATGTGAAATC+AGG - chr5_2:10333081-10333100 Msa0756260:CDS 20.0%
!!! ATAGTTTTACCATTTTTGTT+AGG - chr5_2:10332684-10332703 Msa0756260:intron 20.0%
!! GTAAAATGTTAAATTTCGTA+TGG - chr5_2:10333018-10333037 Msa0756260:intron 20.0%
!!! GATTTAATTTATGTGAAATC+AGG - chr5_2:10333081-10333100 Msa0756260:CDS 20.0%
!!! ATTTTTTTGTACCTAATGTG+TGG + chr5_2:10332635-10332654 None:intergenic 25.0%
! TATAAGAAAACAAAGTGATG+AGG + chr5_2:10332667-10332686 None:intergenic 25.0%
!!! TTTTACCATTTTTGTTAGGT+CGG - chr5_2:10332688-10332707 Msa0756260:intron 25.0%
! AAAAAGTGAAGAAATACCTT+AGG + chr5_2:10332861-10332880 None:intergenic 25.0%
! GAGATGAAGAAAATGATAAA+TGG - chr5_2:10333181-10333200 Msa0756260:CDS 25.0%
!!! ATTTTTTTGTACCTAATGTG+TGG + chr5_2:10332635-10332654 None:intergenic 25.0%
! TATAAGAAAACAAAGTGATG+AGG + chr5_2:10332667-10332686 None:intergenic 25.0%
!!! TTTTACCATTTTTGTTAGGT+CGG - chr5_2:10332688-10332707 Msa0756260:intron 25.0%
! AAAAAGTGAAGAAATACCTT+AGG + chr5_2:10332861-10332880 None:intergenic 25.0%
! GAGATGAAGAAAATGATAAA+TGG - chr5_2:10333181-10333200 Msa0756260:CDS 25.0%
TATTTCATACCATAGACAAC+TGG + chr5_2:10332555-10332574 None:intergenic 30.0%
CCTATTCTATTGAAAAGAAG+AGG + chr5_2:10332582-10332601 None:intergenic 30.0%
! CCTCTTCTTTTCAATAGAAT+AGG - chr5_2:10332579-10332598 Msa0756260:intron 30.0%
TTTCATTATAGCCACACATT+AGG - chr5_2:10332621-10332640 Msa0756260:intron 30.0%
!!! ATTTCTTCACTTTTTACACC+TGG - chr5_2:10332866-10332885 Msa0756260:CDS 30.0%
! TTTTGTTACCCTTAGTTATG+TGG - chr5_2:10332908-10332927 Msa0756260:intron 30.0%
ATCAATGTCCACATAACTAA+GGG + chr5_2:10332919-10332938 None:intergenic 30.0%
TATCAATGTCCACATAACTA+AGG + chr5_2:10332920-10332939 None:intergenic 30.0%
AGTTATGTGGACATTGATAT+AGG - chr5_2:10332921-10332940 Msa0756260:intron 30.0%
ATGTTAAATTTCGTATGGGT+TGG - chr5_2:10333023-10333042 Msa0756260:intron 30.0%
TGTTAAATTTCGTATGGGTT+GGG - chr5_2:10333024-10333043 Msa0756260:intron 30.0%
TATTTCATACCATAGACAAC+TGG + chr5_2:10332555-10332574 None:intergenic 30.0%
CCTATTCTATTGAAAAGAAG+AGG + chr5_2:10332582-10332601 None:intergenic 30.0%
! CCTCTTCTTTTCAATAGAAT+AGG - chr5_2:10332579-10332598 Msa0756260:intron 30.0%
TTTCATTATAGCCACACATT+AGG - chr5_2:10332621-10332640 Msa0756260:intron 30.0%
!!! ATTTCTTCACTTTTTACACC+TGG - chr5_2:10332866-10332885 Msa0756260:CDS 30.0%
! TTTTGTTACCCTTAGTTATG+TGG - chr5_2:10332908-10332927 Msa0756260:intron 30.0%
ATCAATGTCCACATAACTAA+GGG + chr5_2:10332919-10332938 None:intergenic 30.0%
TATCAATGTCCACATAACTA+AGG + chr5_2:10332920-10332939 None:intergenic 30.0%
AGTTATGTGGACATTGATAT+AGG - chr5_2:10332921-10332940 Msa0756260:intron 30.0%
ATGTTAAATTTCGTATGGGT+TGG - chr5_2:10333023-10333042 Msa0756260:intron 30.0%
TGTTAAATTTCGTATGGGTT+GGG - chr5_2:10333024-10333043 Msa0756260:intron 30.0%
!!! AGTGGAATACTGTTTTGATC+TGG + chr5_2:10332469-10332488 None:intergenic 35.0%
! TGAATTGCGTCTGAAATCTT+GGG - chr5_2:10332725-10332744 Msa0756260:intron 35.0%
ACACATACCACAACTTATGA+AGG + chr5_2:10332755-10332774 None:intergenic 35.0%
! GTTGTGGTATGTGTTGTTAA+AGG - chr5_2:10332761-10332780 Msa0756260:intron 35.0%
ATTGATATAGGAAATCGCAG+TGG - chr5_2:10332933-10332952 Msa0756260:intron 35.0%
! GTTAAATTTCGTATGGGTTG+GGG - chr5_2:10333025-10333044 Msa0756260:intron 35.0%
!! TTAAATTTCGTATGGGTTGG+GGG - chr5_2:10333026-10333045 Msa0756260:intron 35.0%
!!! AGTGGAATACTGTTTTGATC+TGG + chr5_2:10332469-10332488 None:intergenic 35.0%
! TGAATTGCGTCTGAAATCTT+GGG - chr5_2:10332725-10332744 Msa0756260:intron 35.0%
ACACATACCACAACTTATGA+AGG + chr5_2:10332755-10332774 None:intergenic 35.0%
! GTTGTGGTATGTGTTGTTAA+AGG - chr5_2:10332761-10332780 Msa0756260:intron 35.0%
ATTGATATAGGAAATCGCAG+TGG - chr5_2:10332933-10332952 Msa0756260:intron 35.0%
! GTTAAATTTCGTATGGGTTG+GGG - chr5_2:10333025-10333044 Msa0756260:intron 35.0%
!! TTAAATTTCGTATGGGTTGG+GGG - chr5_2:10333026-10333045 Msa0756260:intron 35.0%
AAACAGTATTCCACTACAGC+TGG - chr5_2:10332474-10332493 Msa0756260:CDS 40.0%
CAACTCCGACCTAACAAAAA+TGG + chr5_2:10332696-10332715 None:intergenic 40.0%
! CATTTTTGTTAGGTCGGAGT+TGG - chr5_2:10332694-10332713 Msa0756260:intron 40.0%
! CTGAATTGCGTCTGAAATCT+TGG - chr5_2:10332724-10332743 Msa0756260:intron 40.0%
! GTGGTGCTTTTATCAACGTT+TGG - chr5_2:10332955-10332974 Msa0756260:intron 40.0%
!! TAAATTTCGTATGGGTTGGG+GGG - chr5_2:10333027-10333046 Msa0756260:intron 40.0%
AAACAGTATTCCACTACAGC+TGG - chr5_2:10332474-10332493 Msa0756260:CDS 40.0%
CAACTCCGACCTAACAAAAA+TGG + chr5_2:10332696-10332715 None:intergenic 40.0%
! CATTTTTGTTAGGTCGGAGT+TGG - chr5_2:10332694-10332713 Msa0756260:intron 40.0%
! CTGAATTGCGTCTGAAATCT+TGG - chr5_2:10332724-10332743 Msa0756260:intron 40.0%
! GTGGTGCTTTTATCAACGTT+TGG - chr5_2:10332955-10332974 Msa0756260:intron 40.0%
!! TAAATTTCGTATGGGTTGGG+GGG - chr5_2:10333027-10333046 Msa0756260:intron 40.0%
TGCTGTTAGAAACCCTCTTC+CGG - chr5_2:10332497-10332516 Msa0756260:CDS 45.0%
AACGAGACACCAGTTGTCTA+TGG - chr5_2:10332543-10332562 Msa0756260:intron 45.0%
TTTGTTAGGTCGGAGTTGGA+AGG - chr5_2:10332698-10332717 Msa0756260:intron 45.0%
GGGATGACCTTCATAAGTTG+TGG - chr5_2:10332745-10332764 Msa0756260:intron 45.0%
AATACCTTAGGTAAGCGCTC+CGG + chr5_2:10332849-10332868 None:intergenic 45.0%
GATATAGGAAATCGCAGTGG+TGG - chr5_2:10332936-10332955 Msa0756260:intron 45.0%
CAGTAAGCACATGCTTGATG+CGG + chr5_2:10333123-10333142 None:intergenic 45.0%
TGCTGTTAGAAACCCTCTTC+CGG - chr5_2:10332497-10332516 Msa0756260:CDS 45.0%
AACGAGACACCAGTTGTCTA+TGG - chr5_2:10332543-10332562 Msa0756260:intron 45.0%
TTTGTTAGGTCGGAGTTGGA+AGG - chr5_2:10332698-10332717 Msa0756260:intron 45.0%
GGGATGACCTTCATAAGTTG+TGG - chr5_2:10332745-10332764 Msa0756260:intron 45.0%
AATACCTTAGGTAAGCGCTC+CGG + chr5_2:10332849-10332868 None:intergenic 45.0%
GATATAGGAAATCGCAGTGG+TGG - chr5_2:10332936-10332955 Msa0756260:intron 45.0%
CAGTAAGCACATGCTTGATG+CGG + chr5_2:10333123-10333142 None:intergenic 45.0%
TCTAACAGCACCAGCTGTAG+TGG + chr5_2:10332487-10332506 None:intergenic 50.0%
CCTCAAAGAACTCCGGAAGA+GGG + chr5_2:10332512-10332531 None:intergenic 50.0%
CTGTCTGCCTCAAAGAACTC+CGG + chr5_2:10332519-10332538 None:intergenic 50.0%
GAACTTGCGCTTTCCTAACC+CGG - chr5_2:10332827-10332846 Msa0756260:CDS 50.0%
ATACCTTAGGTAAGCGCTCC+GGG + chr5_2:10332848-10332867 None:intergenic 50.0%
CAACACGCGTAAATCAGACC+AGG + chr5_2:10332887-10332906 None:intergenic 50.0%
CGATTGAAGAACCAGACGCA+AGG - chr5_2:10333149-10333168 Msa0756260:CDS 50.0%
TTGAAGAACCAGACGCAAGG+AGG - chr5_2:10333152-10333171 Msa0756260:CDS 50.0%
TCTAACAGCACCAGCTGTAG+TGG + chr5_2:10332487-10332506 None:intergenic 50.0%
CCTCAAAGAACTCCGGAAGA+GGG + chr5_2:10332512-10332531 None:intergenic 50.0%
CTGTCTGCCTCAAAGAACTC+CGG + chr5_2:10332519-10332538 None:intergenic 50.0%
GAACTTGCGCTTTCCTAACC+CGG - chr5_2:10332827-10332846 Msa0756260:CDS 50.0%
ATACCTTAGGTAAGCGCTCC+GGG + chr5_2:10332848-10332867 None:intergenic 50.0%
CAACACGCGTAAATCAGACC+AGG + chr5_2:10332887-10332906 None:intergenic 50.0%
CGATTGAAGAACCAGACGCA+AGG - chr5_2:10333149-10333168 Msa0756260:CDS 50.0%
TTGAAGAACCAGACGCAAGG+AGG - chr5_2:10333152-10333171 Msa0756260:CDS 50.0%
CCCTCTTCCGGAGTTCTTTG+AGG - chr5_2:10332509-10332528 Msa0756260:intron 55.0%
GCCTCAAAGAACTCCGGAAG+AGG + chr5_2:10332513-10332532 None:intergenic 55.0%
TTAGGTAAGCGCTCCGGGTT+AGG + chr5_2:10332843-10332862 None:intergenic 55.0%
! TAACCCGGAGCGCTTACCTA+AGG - chr5_2:10332842-10332861 Msa0756260:CDS 55.0%
GGTTGGGGGGAAACTGTTCA+CGG - chr5_2:10333040-10333059 Msa0756260:intron 55.0%
CCCTCTTCCGGAGTTCTTTG+AGG - chr5_2:10332509-10332528 Msa0756260:intron 55.0%
GCCTCAAAGAACTCCGGAAG+AGG + chr5_2:10332513-10332532 None:intergenic 55.0%
TTAGGTAAGCGCTCCGGGTT+AGG + chr5_2:10332843-10332862 None:intergenic 55.0%
! TAACCCGGAGCGCTTACCTA+AGG - chr5_2:10332842-10332861 Msa0756260:CDS 55.0%
GGTTGGGGGGAAACTGTTCA+CGG - chr5_2:10333040-10333059 Msa0756260:intron 55.0%
TCAGCAGACCTCCTTGCGTC+TGG + chr5_2:10333163-10333182 None:intergenic 60.0%
TCAGCAGACCTCCTTGCGTC+TGG + chr5_2:10333163-10333182 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 10332420 10333210 10332420 ID=Msa0756260;Name=Msa0756260
chr5_2 mRNA 10332420 10333210 10332420 ID=Msa0756260-mRNA-1;Parent=Msa0756260;Name=Msa0756260-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|136
chr5_2 exon 10333066 10333210 10333066 ID=Msa0756260-mRNA-1:exon:4739;Parent=Msa0756260-mRNA-1
chr5_2 exon 10332767 10332924 10332767 ID=Msa0756260-mRNA-1:exon:4738;Parent=Msa0756260-mRNA-1
chr5_2 exon 10332420 10332527 10332420 ID=Msa0756260-mRNA-1:exon:4737;Parent=Msa0756260-mRNA-1
chr5_2 CDS 10333066 10333210 10333066 ID=Msa0756260-mRNA-1:cds;Parent=Msa0756260-mRNA-1
chr5_2 CDS 10332767 10332924 10332767 ID=Msa0756260-mRNA-1:cds;Parent=Msa0756260-mRNA-1
chr5_2 CDS 10332420 10332527 10332420 ID=Msa0756260-mRNA-1:cds;Parent=Msa0756260-mRNA-1
Gene Sequence

>Msa0756260

ATGTTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCAAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA

Protein sequence

>Msa0756260

MFLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL*